Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028895.t1 | RHN75739.1 | 84.3 | 159 | 22 | 2 | 1 | 156 | 1 | 159 | 1.40E-61 | 245.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028895.t1 | F4JIJ7 | 44.3 | 149 | 73 | 4 | 12 | 151 | 11 | 158 | 4.5e-21 | 102.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028895.t1 | A0A072VBG7 | 84.3 | 159 | 22 | 2 | 1 | 156 | 1 | 159 | 9.9e-62 | 245.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene028895.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028895.t1 | MTR_2g090440 | 84.713 | 157 | 22 | 2 | 1 | 156 | 1 | 156 | 5.30e-91 | 263 |
| MS.gene028895.t1 | MTR_2g090425 | 84.277 | 159 | 22 | 2 | 1 | 156 | 1 | 159 | 4.98e-90 | 265 |
| MS.gene028895.t1 | MTR_2g090480 | 85.000 | 160 | 18 | 4 | 1 | 156 | 1 | 158 | 8.86e-90 | 261 |
| MS.gene028895.t1 | MTR_4g078350 | 61.783 | 157 | 47 | 4 | 1 | 151 | 1 | 150 | 9.37e-59 | 181 |
| MS.gene028895.t1 | MTR_2g090500 | 83.784 | 111 | 17 | 1 | 46 | 156 | 60 | 169 | 9.97e-59 | 182 |
| MS.gene028895.t1 | MTR_2g013920 | 44.218 | 147 | 80 | 2 | 10 | 156 | 5 | 149 | 1.94e-35 | 121 |
| MS.gene028895.t1 | MTR_2g090515 | 72.727 | 55 | 15 | 0 | 1 | 55 | 1 | 55 | 2.30e-21 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028895.t1 | AT4G20970 | 40.909 | 154 | 81 | 3 | 12 | 156 | 11 | 163 | 1.81e-28 | 104 |
| MS.gene028895.t1 | AT1G10585 | 29.932 | 147 | 92 | 3 | 11 | 156 | 13 | 149 | 1.10e-12 | 62.8 |
Find 27 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTCTTCAAATTCTTCTT+TGG | 0.236554 | 2.3:-10025957 | MS.gene028895:CDS |
| GGAGAAGAAAGAAAGTTTAA+TGG | 0.269701 | 2.3:-10026158 | MS.gene028895:CDS |
| GGTGGCTGCTCGAGTTCTTC+TGG | 0.283108 | 2.3:-10026112 | MS.gene028895:CDS |
| GAGAGTTGAGTTTAGAAAAG+AGG | 0.353043 | 2.3:+10026990 | None:intergenic |
| GAGAAGAAAGAAAGTTTAAT+GGG | 0.362015 | 2.3:-10026157 | MS.gene028895:CDS |
| CTAAGTTTATATTTGTCTCT+AGG | 0.365336 | 2.3:+10026185 | None:intergenic |
| TAGAAAAGAGGATCTTCATT+TGG | 0.386590 | 2.3:+10027002 | None:intergenic |
| AACGATCTTTCTTTCGACTT+TGG | 0.395490 | 2.3:+10027042 | None:intergenic |
| CTTTCGACTTTGGTTGAAGA+AGG | 0.411324 | 2.3:+10027052 | None:intergenic |
| TCTTCAAATTCTTCTTTGGT+TGG | 0.413497 | 2.3:-10025953 | MS.gene028895:CDS |
| TAATTTCACAGAAAATAAAA+TGG | 0.425918 | 2.3:+10026011 | None:intergenic |
| ATAGCCTCATCTACTTGATC+AGG | 0.429867 | 2.3:+10026223 | None:intergenic |
| AAAATAGAGATTCATGAAAT+GGG | 0.456591 | 2.3:-10026073 | MS.gene028895:CDS |
| ATCACCTTTGGATTATAGCT+AGG | 0.496982 | 2.3:+10026962 | None:intergenic |
| AAGAAGAGATCGCGTGGTGG+TGG | 0.500238 | 2.3:-10026130 | MS.gene028895:CDS |
| TCACCTTTGGATTATAGCTA+GGG | 0.515824 | 2.3:+10026963 | None:intergenic |
| TCATCTACTTGATCAGGTAA+TGG | 0.522136 | 2.3:+10026229 | None:intergenic |
| GACAAATATAAACTTAGCAA+AGG | 0.562346 | 2.3:-10026179 | MS.gene028895:CDS |
| ACAAGTCATTGTAACATGTA+GGG | 0.567604 | 2.3:-10026041 | MS.gene028895:CDS |
| TACAAGTCATTGTAACATGT+AGG | 0.579023 | 2.3:-10026042 | MS.gene028895:CDS |
| TCTCCCTAGCTATAATCCAA+AGG | 0.625430 | 2.3:-10026966 | MS.gene028895:intron |
| TGTTACAATGACTTGTAGAG+TGG | 0.628096 | 2.3:+10026048 | None:intergenic |
| ATTACCTGATCAAGTAGATG+AGG | 0.636526 | 2.3:-10026227 | MS.gene028895:CDS |
| TCAATGGATCAAAATCAGCA+AGG | 0.641836 | 2.3:-10027082 | MS.gene028895:CDS |
| GGAAACAAGAAGAGATCGCG+TGG | 0.678742 | 2.3:-10026136 | MS.gene028895:CDS |
| AACAAGAAGAGATCGCGTGG+TGG | 0.717188 | 2.3:-10026133 | MS.gene028895:CDS |
| ACTGCATGAAGAGAACGTCG+AGG | 0.768331 | 2.3:-10025981 | MS.gene028895:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATATTTTTTTTGTTATGT+AGG | + | chr2.3:10026524-10026543 | None:intergenic | 10.0% |
| !! | TAATTTCACAGAAAATAAAA+TGG | + | chr2.3:10026999-10027018 | None:intergenic | 15.0% |
| !! | AAAAATAGAGATTCATGAAA+TGG | - | chr2.3:10026933-10026952 | MS.gene028895:intron | 20.0% |
| !! | AAAATAGAGATTCATGAAAT+GGG | - | chr2.3:10026934-10026953 | MS.gene028895:intron | 20.0% |
| !! | AGCACATATACTTATTAATT+TGG | - | chr2.3:10026721-10026740 | MS.gene028895:intron | 20.0% |
| !! | CATATATGTATATTCATTTG+AGG | - | chr2.3:10026095-10026114 | MS.gene028895:CDS | 20.0% |
| !! | CCTATATATTCATTTCATAT+AGG | - | chr2.3:10026676-10026695 | MS.gene028895:intron | 20.0% |
| !! | CCTATATGAAATGAATATAT+AGG | + | chr2.3:10026679-10026698 | None:intergenic | 20.0% |
| !! | TGACAAAAATAAAGAGAAAA+TGG | + | chr2.3:10026479-10026498 | None:intergenic | 20.0% |
| !!! | ATTTCATGAATCTCTATTTT+TGG | + | chr2.3:10026934-10026953 | None:intergenic | 20.0% |
| !!! | TGTTATTTTTCATTTAATGC+AGG | - | chr2.3:10026744-10026763 | MS.gene028895:intron | 20.0% |
| ! | AAAGAGAAAATGGATAAGTT+AGG | + | chr2.3:10026469-10026488 | None:intergenic | 25.0% |
| ! | AATTTACACTTAGAATGTAC+AGG | - | chr2.3:10026283-10026302 | MS.gene028895:intron | 25.0% |
| ! | ATATATGCATATGAATGTGA+TGG | + | chr2.3:10026082-10026101 | None:intergenic | 25.0% |
| ! | ATTTACACTTAGAATGTACA+GGG | - | chr2.3:10026284-10026303 | MS.gene028895:intron | 25.0% |
| ! | CTAAGTTTATATTTGTCTCT+AGG | + | chr2.3:10026825-10026844 | None:intergenic | 25.0% |
| ! | GACAAATATAAACTTAGCAA+AGG | - | chr2.3:10026828-10026847 | MS.gene028895:intron | 25.0% |
| ! | GAGAAGAAAGAAAGTTTAAT+GGG | - | chr2.3:10026850-10026869 | MS.gene028895:intron | 25.0% |
| ! | TATATAGTTACCAATCTCAA+AGG | + | chr2.3:10026173-10026192 | None:intergenic | 25.0% |
| ! | TATCTCTTCAAATTCTTCTT+TGG | - | chr2.3:10027050-10027069 | MS.gene028895:CDS | 25.0% |
| ! | TGTACATTCTAAGTGTAAAT+TGG | + | chr2.3:10026284-10026303 | None:intergenic | 25.0% |
| !! | CTCGGTTCTAATATTAAAAA+TGG | - | chr2.3:10026328-10026347 | MS.gene028895:intron | 25.0% |
| !!! | AATTGGTTTTACATCAACAA+CGG | + | chr2.3:10026267-10026286 | None:intergenic | 25.0% |
| !!! | TCAATGTCGTTTTTTTTTAC+AGG | + | chr2.3:10026146-10026165 | None:intergenic | 25.0% |
| ACAAGTCATTGTAACATGTA+GGG | - | chr2.3:10026966-10026985 | MS.gene028895:intron | 30.0% | |
| ATATATAGGTACTCTACTTG+TGG | + | chr2.3:10026665-10026684 | None:intergenic | 30.0% | |
| GAAAGATCGTTGAGAAAAAT+AGG | - | chr2.3:10025977-10025996 | MS.gene028895:CDS | 30.0% | |
| GAAATTAAACAGATCACCTT+TGG | + | chr2.3:10026060-10026079 | None:intergenic | 30.0% | |
| GGAGAAGAAAGAAAGTTTAA+TGG | - | chr2.3:10026849-10026868 | MS.gene028895:intron | 30.0% | |
| TACAAGTCATTGTAACATGT+AGG | - | chr2.3:10026965-10026984 | MS.gene028895:intron | 30.0% | |
| TAGAAAAGAGGATCTTCATT+TGG | + | chr2.3:10026008-10026027 | None:intergenic | 30.0% | |
| TCTTCAAATTCTTCTTTGGT+TGG | - | chr2.3:10027054-10027073 | MS.gene028895:CDS | 30.0% | |
| TTCCAATAAAATTGGAGACT+CGG | - | chr2.3:10026310-10026329 | MS.gene028895:intron | 30.0% | |
| ! | AGTCTCCAATTTTATTGGAA+CGG | + | chr2.3:10026310-10026329 | None:intergenic | 30.0% |
| ! | TACATGACTCTTTGTTGTAT+TGG | - | chr2.3:10026231-10026250 | MS.gene028895:CDS | 30.0% |
| !! | TTTGTTGTTGATAGATGTGT+GGG | - | chr2.3:10026438-10026457 | MS.gene028895:intron | 30.0% |
| !!! | TTTTGTTGTTGATAGATGTG+TGG | - | chr2.3:10026437-10026456 | MS.gene028895:intron | 30.0% |
| AACGATCTTTCTTTCGACTT+TGG | + | chr2.3:10025968-10025987 | None:intergenic | 35.0% | |
| ATCACCTTTGGATTATAGCT+AGG | + | chr2.3:10026048-10026067 | None:intergenic | 35.0% | |
| ATTACCTGATCAAGTAGATG+AGG | - | chr2.3:10026780-10026799 | MS.gene028895:intron | 35.0% | |
| CATATAGGTTCACTTCTTCT+TGG | - | chr2.3:10026691-10026710 | MS.gene028895:intron | 35.0% | |
| GAGAGTTGAGTTTAGAAAAG+AGG | + | chr2.3:10026020-10026039 | None:intergenic | 35.0% | |
| GAGTATAATTGTTGGGATCT+AGG | + | chr2.3:10026548-10026567 | None:intergenic | 35.0% | |
| TAATTGAGGACCCCATAAAT+TGG | - | chr2.3:10026581-10026600 | MS.gene028895:intron | 35.0% | |
| TCACCTTTGGATTATAGCTA+GGG | + | chr2.3:10026047-10026066 | None:intergenic | 35.0% | |
| TCATCTACTTGATCAGGTAA+TGG | + | chr2.3:10026781-10026800 | None:intergenic | 35.0% | |
| ! | AACCGAGTCTCCAATTTTAT+TGG | + | chr2.3:10026315-10026334 | None:intergenic | 35.0% |
| ! | TGTTACAATGACTTGTAGAG+TGG | + | chr2.3:10026962-10026981 | None:intergenic | 35.0% |
| !! | CATTGAGTTTCCTTTGAGAT+TGG | - | chr2.3:10026160-10026179 | MS.gene028895:CDS | 35.0% |
| ATAGCCTCATCTACTTGATC+AGG | + | chr2.3:10026787-10026806 | None:intergenic | 40.0% | |
| CGTACCCCTACAAATAATTG+AGG | - | chr2.3:10026567-10026586 | MS.gene028895:intron | 40.0% | |
| CTTGTGGTATAGTACACTCA+AGG | + | chr2.3:10026649-10026668 | None:intergenic | 40.0% | |
| CTTTCGACTTTGGTTGAAGA+AGG | + | chr2.3:10025958-10025977 | None:intergenic | 40.0% | |
| GGGGTACGAGTATAATTGTT+GGG | + | chr2.3:10026555-10026574 | None:intergenic | 40.0% | |
| GGGGTCCTCAATTATTTGTA+GGG | + | chr2.3:10026575-10026594 | None:intergenic | 40.0% | |
| GGGTCCTCAATTATTTGTAG+GGG | + | chr2.3:10026574-10026593 | None:intergenic | 40.0% | |
| TCTCCCTAGCTATAATCCAA+AGG | - | chr2.3:10026041-10026060 | MS.gene028895:CDS | 40.0% | |
| TGGGGTCCTCAATTATTTGT+AGG | + | chr2.3:10026576-10026595 | None:intergenic | 40.0% | |
| ! | AGGGGTACGAGTATAATTGT+TGG | + | chr2.3:10026556-10026575 | None:intergenic | 40.0% |
| ! | TGTTGATAGATGTGTGGGTT+TGG | - | chr2.3:10026443-10026462 | MS.gene028895:intron | 40.0% |
| !! | CTTTGTTGTATTGGCTAGCT+GGG | - | chr2.3:10026240-10026259 | MS.gene028895:CDS | 40.0% |
| !! | TCTTTGTTGTATTGGCTAGC+TGG | - | chr2.3:10026239-10026258 | MS.gene028895:CDS | 40.0% |
| CAGGGCCGTTCCAATAAAAT+TGG | - | chr2.3:10026302-10026321 | MS.gene028895:intron | 45.0% | |
| TTGAGGACCCCATAAATTGG+AGG | - | chr2.3:10026584-10026603 | MS.gene028895:intron | 45.0% | |
| !!! | ATCATTAATTATTTTTATTA+GGG | + | chr2.3:10026355-10026374 | None:intergenic | 5.0% |
| !!! | TATCATTAATTATTTTTATT+AGG | + | chr2.3:10026356-10026375 | None:intergenic | 5.0% |
| AACAAGAAGAGATCGCGTGG+TGG | - | chr2.3:10026874-10026893 | MS.gene028895:intron | 50.0% | |
| ACTGCATGAAGAGAACGTCG+AGG | - | chr2.3:10027026-10027045 | MS.gene028895:CDS | 50.0% | |
| AGCCGGACCTCCAATTTATG+GGG | + | chr2.3:10026594-10026613 | None:intergenic | 50.0% | |
| GACCCCATAAATTGGAGGTC+CGG | - | chr2.3:10026589-10026608 | MS.gene028895:intron | 50.0% | |
| GAGCCGGACCTCCAATTTAT+GGG | + | chr2.3:10026595-10026614 | None:intergenic | 50.0% | |
| GGAAACAAGAAGAGATCGCG+TGG | - | chr2.3:10026871-10026890 | MS.gene028895:intron | 50.0% | |
| AAGAAGAGATCGCGTGGTGG+TGG | - | chr2.3:10026877-10026896 | MS.gene028895:intron | 55.0% | |
| GGAGCCGGACCTCCAATTTA+TGG | + | chr2.3:10026596-10026615 | None:intergenic | 55.0% | |
| GTACACTCAAGGCCGTTCCT+TGG | + | chr2.3:10026638-10026657 | None:intergenic | 55.0% | |
| TCCTTGCGCTTGCCAAGGAA+CGG | - | chr2.3:10026623-10026642 | MS.gene028895:intron | 55.0% | |
| AGAGCTCCTTGCGCTTGCCA+AGG | - | chr2.3:10026618-10026637 | MS.gene028895:intron | 60.0% | |
| GGCAAGCGCAAGGAGCTCTA+CGG | + | chr2.3:10026617-10026636 | None:intergenic | 60.0% | |
| GGTGGCTGCTCGAGTTCTTC+TGG | - | chr2.3:10026895-10026914 | MS.gene028895:intron | 60.0% | |
| CGCAAGGAGCTCTACGGAGC+CGG | + | chr2.3:10026611-10026630 | None:intergenic | 65.0% | |
| GCCGTTCCTTGGCAAGCGCA+AGG | + | chr2.3:10026627-10026646 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 10025928 | 10027101 | 10025928 | ID=MS.gene028895 |
| chr2.3 | mRNA | 10025928 | 10027101 | 10025928 | ID=MS.gene028895.t1;Parent=MS.gene028895 |
| chr2.3 | exon | 10026967 | 10027101 | 10026967 | ID=MS.gene028895.t1.exon1;Parent=MS.gene028895.t1 |
| chr2.3 | CDS | 10026967 | 10027101 | 10026967 | ID=cds.MS.gene028895.t1;Parent=MS.gene028895.t1 |
| chr2.3 | exon | 10025928 | 10026263 | 10025928 | ID=MS.gene028895.t1.exon2;Parent=MS.gene028895.t1 |
| chr2.3 | CDS | 10025928 | 10026263 | 10025928 | ID=cds.MS.gene028895.t1;Parent=MS.gene028895.t1 |
| Gene Sequence |
| Protein sequence |
