Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029018.t1 | XP_003597068.1 | 63.5 | 233 | 12 | 1 | 1 | 160 | 1 | 233 | 6.40E-62 | 246.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029018.t1 | Q9LRM4 | 56.0 | 50 | 22 | 0 | 5 | 54 | 31 | 80 | 5.3e-09 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029018.t1 | G7IRL0 | 63.5 | 233 | 12 | 1 | 1 | 160 | 1 | 233 | 4.6e-62 | 246.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene029018.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029018.t1 | MTR_2g089310 | 92.208 | 77 | 6 | 0 | 84 | 160 | 157 | 233 | 1.87e-46 | 151 |
| MS.gene029018.t1 | MTR_2g089310 | 98.438 | 64 | 1 | 0 | 1 | 64 | 1 | 64 | 8.93e-42 | 139 |
| MS.gene029018.t1 | MTR_2g011450 | 67.188 | 64 | 21 | 0 | 1 | 64 | 1 | 64 | 7.21e-28 | 100 |
| MS.gene029018.t1 | MTR_4g046640 | 46.377 | 69 | 37 | 0 | 5 | 73 | 36 | 104 | 5.63e-14 | 65.1 |
| MS.gene029018.t1 | MTR_4g008050 | 50.820 | 61 | 30 | 0 | 3 | 63 | 6 | 66 | 2.23e-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029018.t1 | AT5G54470 | 74.603 | 63 | 16 | 0 | 2 | 64 | 3 | 65 | 2.79e-28 | 104 |
| MS.gene029018.t1 | AT4G27310 | 70.312 | 64 | 19 | 0 | 1 | 64 | 1 | 64 | 9.89e-28 | 103 |
| MS.gene029018.t1 | AT3G21890 | 41.860 | 86 | 49 | 1 | 5 | 90 | 31 | 115 | 1.11e-11 | 58.9 |
Find 41 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGCGGTATAGCGACGTAAA+TGG | 0.268815 | 2.3:-11672475 | MS.gene029018:CDS |
| ATGCTGGGACTGCGATTCTA+AGG | 0.346631 | 2.3:-11673156 | MS.gene029018:CDS |
| GTGGATGAGGTTGATTTGAT+AGG | 0.378300 | 2.3:-11672532 | MS.gene029018:CDS |
| CGTGGAAAGCCTCCGGCGCT+AGG | 0.440306 | 2.3:-11673061 | MS.gene029018:CDS |
| CGTCGTCAAAGGCGGCGATT+CGG | 0.463533 | 2.3:-11672495 | MS.gene029018:CDS |
| CGAATCGCCGCCTTTGACGA+CGG | 0.469995 | 2.3:+11672496 | None:intergenic |
| CCGACCAGGCTAGCTTATGC+TGG | 0.471766 | 2.3:-11673172 | MS.gene029018:CDS |
| CGCGTTACCGAGCCTAGCGC+CGG | 0.498391 | 2.3:+11673049 | None:intergenic |
| CCCACGCCGTGGAAAGCCTC+CGG | 0.526413 | 2.3:-11673068 | MS.gene029018:CDS |
| GACTCACAGAAAGTTCTAGC+TGG | 0.526494 | 2.3:+11673194 | None:intergenic |
| AGTCCCAGCATAAGCTAGCC+TGG | 0.526991 | 2.3:+11673168 | None:intergenic |
| AACTTTCTGTGAGTCCGACC+AGG | 0.531925 | 2.3:-11673186 | MS.gene029018:CDS |
| CCACCGGCTTCTAGTTCCTC+CGG | 0.538845 | 2.3:-11672813 | MS.gene029018:CDS |
| TGGATGAGGTTGATTTGATA+GGG | 0.541461 | 2.3:-11672531 | MS.gene029018:CDS |
| GCCGGAGGCTTTCCACGGCG+TGG | 0.543692 | 2.3:+11673067 | None:intergenic |
| CGACGAGGAACCGTCGTCAA+AGG | 0.547277 | 2.3:-11672506 | MS.gene029018:CDS |
| TTCCTCCGGTAGCGAAGAGT+CGG | 0.547879 | 2.3:-11672799 | MS.gene029018:CDS |
| AAAGCCTCCGGCGCTAGGCT+CGG | 0.551924 | 2.3:-11673056 | MS.gene029018:intron |
| AACAGCGGCGCAACCACCAC+CGG | 0.553693 | 2.3:-11672829 | MS.gene029018:intron |
| CGACCAGGCTAGCTTATGCT+GGG | 0.557426 | 2.3:-11673171 | MS.gene029018:CDS |
| GTTACCGAGCCTAGCGCCGG+AGG | 0.559866 | 2.3:+11673052 | None:intergenic |
| CGTCAAAGGCGGCGATTCGG+CGG | 0.566085 | 2.3:-11672492 | MS.gene029018:CDS |
| CCGGAGGCTTTCCACGGCGT+GGG | 0.569147 | 2.3:+11673068 | None:intergenic |
| CATTCTCAGCCACACGATTG+AGG | 0.570452 | 2.3:-11672445 | None:intergenic |
| CTAGCGCCGGAGGCTTTCCA+CGG | 0.572274 | 2.3:+11673062 | None:intergenic |
| CGTAACCGACTCTTCGCTAC+CGG | 0.573854 | 2.3:+11672794 | None:intergenic |
| TCAACGAAGAGAGGTGGATG+AGG | 0.575020 | 2.3:-11672545 | MS.gene029018:CDS |
| CCAGCATAAGCTAGCCTGGT+CGG | 0.605872 | 2.3:+11673172 | None:intergenic |
| CGGTAGCGAAGAGTCGGTTA+CGG | 0.606860 | 2.3:-11672793 | MS.gene029018:CDS |
| CGAGGAACCGTCGTCAAAGG+CGG | 0.613549 | 2.3:-11672503 | MS.gene029018:CDS |
| CTACCGGAGGAACTAGAAGC+CGG | 0.616312 | 2.3:+11672810 | None:intergenic |
| GAGGAACTAGAAGCCGGTGG+TGG | 0.618905 | 2.3:+11672816 | None:intergenic |
| GAGATTGACATGCGTGACAG+AGG | 0.627483 | 2.3:+11673090 | None:intergenic |
| GTATAGCGACGTAAATGGAA+CGG | 0.632422 | 2.3:-11672470 | MS.gene029018:CDS |
| AACCGACTCTTCGCTACCGG+AGG | 0.640395 | 2.3:+11672797 | None:intergenic |
| CATGTCAATCTCCCACGCCG+TGG | 0.644127 | 2.3:-11673079 | MS.gene029018:CDS |
| ATAGTACGGCCTCAATCGTG+TGG | 0.647407 | 2.3:+11672436 | None:intergenic |
| CCGGAGGAACTAGAAGCCGG+TGG | 0.696282 | 2.3:+11672813 | None:intergenic |
| TGATTTGATAGGGTGCGACG+AGG | 0.704245 | 2.3:-11672521 | MS.gene029018:CDS |
| GTGCAACGATAACCACGATG+AGG | 0.723607 | 2.3:-11672763 | MS.gene029018:CDS |
| ACTTAGAAATAACCTCATCG+TGG | 0.742114 | 2.3:+11672751 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTGTTGATTTTGTTTTC+AGG | - | chr2.3:11673078-11673097 | MS.gene029018:CDS | 20.0% |
| !!! | TTGATTTTGTTTTCAGGTTA+CGG | - | chr2.3:11673084-11673103 | MS.gene029018:CDS | 25.0% |
| AGGTTACGGATTCAAAAAAT+TGG | - | chr2.3:11673098-11673117 | MS.gene029018:CDS | 30.0% | |
| ! | TCGTTTAGATCATGATTTTC+AGG | - | chr2.3:11672971-11672990 | MS.gene029018:intron | 30.0% |
| ACTTAGAAATAACCTCATCG+TGG | + | chr2.3:11672923-11672942 | None:intergenic | 35.0% | |
| TGAAGAAGATGGTGATAATC+AGG | - | chr2.3:11672803-11672822 | MS.gene029018:CDS | 35.0% | |
| TTCACTTTCTTCTTGTTGCT+CGG | + | chr2.3:11672721-11672740 | None:intergenic | 35.0% | |
| TTGGAAAAATCAACGAAGAG+AGG | - | chr2.3:11673117-11673136 | MS.gene029018:CDS | 35.0% | |
| !! | TGGATGAGGTTGATTTGATA+GGG | - | chr2.3:11673140-11673159 | MS.gene029018:CDS | 35.0% |
| GAAAAATCAACGAAGAGAGG+TGG | - | chr2.3:11673120-11673139 | MS.gene029018:CDS | 40.0% | |
| GAGCAACAAGAAGAAAGTGA+AGG | - | chr2.3:11672720-11672739 | MS.gene029018:CDS | 40.0% | |
| GTATAGCGACGTAAATGGAA+CGG | - | chr2.3:11673201-11673220 | MS.gene029018:CDS | 40.0% | |
| TTCCTCATCGGAATCGTAAT+CGG | + | chr2.3:11672769-11672788 | None:intergenic | 40.0% | |
| !! | GTGGATGAGGTTGATTTGAT+AGG | - | chr2.3:11673139-11673158 | MS.gene029018:CDS | 40.0% |
| ! | GACTCACAGAAAGTTCTAGC+TGG | + | chr2.3:11672480-11672499 | None:intergenic | 45.0% |
| ! | GGTTCACGCTGCTAACTTTT+TGG | - | chr2.3:11672536-11672555 | MS.gene029018:CDS | 45.0% |
| ! | GTGATAATCAGGTTGTTCCG+TGG | - | chr2.3:11672814-11672833 | MS.gene029018:CDS | 45.0% |
| AACTTTCTGTGAGTCCGACC+AGG | - | chr2.3:11672485-11672504 | MS.gene029018:CDS | 50.0% | |
| ATGCTGGGACTGCGATTCTA+AGG | - | chr2.3:11672515-11672534 | MS.gene029018:CDS | 50.0% | |
| CGATTCCGATGAGGAAGATG+AGG | - | chr2.3:11672773-11672792 | MS.gene029018:CDS | 50.0% | |
| CTCGTCCTCATCTTCCTCAT+CGG | + | chr2.3:11672781-11672800 | None:intergenic | 50.0% | |
| GAGATTGACATGCGTGACAG+AGG | + | chr2.3:11672584-11672603 | None:intergenic | 50.0% | |
| GAGGACGAGGATGAAGAAGA+TGG | - | chr2.3:11672792-11672811 | MS.gene029018:CDS | 50.0% | |
| GTGCAACGATAACCACGATG+AGG | - | chr2.3:11672908-11672927 | MS.gene029018:intron | 50.0% | |
| TCAACGAAGAGAGGTGGATG+AGG | - | chr2.3:11673126-11673145 | MS.gene029018:CDS | 50.0% | |
| TCGTTGTGTGATAGATGCGC+CGG | - | chr2.3:11672648-11672667 | MS.gene029018:intron | 50.0% | |
| TGATTTGATAGGGTGCGACG+AGG | - | chr2.3:11673150-11673169 | MS.gene029018:CDS | 50.0% | |
| TGTTCCGTGGTCATCAACAG+CGG | - | chr2.3:11672827-11672846 | MS.gene029018:CDS | 50.0% | |
| ! | CACCGATTACGATTCCGATG+AGG | - | chr2.3:11672764-11672783 | MS.gene029018:CDS | 50.0% |
| AGTCCCAGCATAAGCTAGCC+TGG | + | chr2.3:11672506-11672525 | None:intergenic | 55.0% | |
| CGATGAGGAAGATGAGGACG+AGG | - | chr2.3:11672779-11672798 | MS.gene029018:CDS | 55.0% | |
| CGGCGGTATAGCGACGTAAA+TGG | - | chr2.3:11673196-11673215 | MS.gene029018:CDS | 55.0% | |
| CGGTAGCGAAGAGTCGGTTA+CGG | - | chr2.3:11672878-11672897 | MS.gene029018:intron | 55.0% | |
| CTACCGGAGGAACTAGAAGC+CGG | + | chr2.3:11672864-11672883 | None:intergenic | 55.0% | |
| TTCCTCCGGTAGCGAAGAGT+CGG | - | chr2.3:11672872-11672891 | MS.gene029018:intron | 55.0% | |
| TTCTTCTTGTTGCTCGGCGC+TGG | + | chr2.3:11672715-11672734 | None:intergenic | 55.0% | |
| TTGTGTGATAGATGCGCCGG+CGG | - | chr2.3:11672651-11672670 | MS.gene029018:intron | 55.0% | |
| ! | CCAGCATAAGCTAGCCTGGT+CGG | + | chr2.3:11672502-11672521 | None:intergenic | 55.0% |
| ! | CGTAACCGACTCTTCGCTAC+CGG | + | chr2.3:11672880-11672899 | None:intergenic | 55.0% |
| !! | CGACCAGGCTAGCTTATGCT+GGG | - | chr2.3:11672500-11672519 | MS.gene029018:CDS | 55.0% |
| CATGTCAATCTCCCACGCCG+TGG | - | chr2.3:11672592-11672611 | MS.gene029018:intron | 60.0% | |
| CGAATCGCCGCCTTTGACGA+CGG | + | chr2.3:11673178-11673197 | None:intergenic | 60.0% | |
| CGACGAGGAACCGTCGTCAA+AGG | - | chr2.3:11673165-11673184 | MS.gene029018:CDS | 60.0% | |
| CGAGGAACCGTCGTCAAAGG+CGG | - | chr2.3:11673168-11673187 | MS.gene029018:CDS | 60.0% | |
| CGTCGTCAAAGGCGGCGATT+CGG | - | chr2.3:11673176-11673195 | MS.gene029018:CDS | 60.0% | |
| GAGGAACTAGAAGCCGGTGG+TGG | + | chr2.3:11672858-11672877 | None:intergenic | 60.0% | |
| GCCAACGCTAACTCCAACGC+CGG | - | chr2.3:11672687-11672706 | MS.gene029018:intron | 60.0% | |
| ! | AACCGACTCTTCGCTACCGG+AGG | + | chr2.3:11672877-11672896 | None:intergenic | 60.0% |
| ! | ACCGGCGTTGGAGTTAGCGT+TGG | + | chr2.3:11672691-11672710 | None:intergenic | 60.0% |
| ! | GGCGTGGAGTTTTCTTCCGC+CGG | + | chr2.3:11672670-11672689 | None:intergenic | 60.0% |
| !! | CCACCGGCTTCTAGTTCCTC+CGG | - | chr2.3:11672858-11672877 | MS.gene029018:intron | 60.0% |
| !! | CCGACCAGGCTAGCTTATGC+TGG | - | chr2.3:11672499-11672518 | MS.gene029018:CDS | 60.0% |
| !! | CGTTGGAGTTAGCGTTGGCG+TGG | + | chr2.3:11672686-11672705 | None:intergenic | 60.0% |
| !! | TGCGCCGCTGTTGATGACCA+CGG | + | chr2.3:11672834-11672853 | None:intergenic | 60.0% |
| AAAGCCTCCGGCGCTAGGCT+CGG | - | chr2.3:11672615-11672634 | MS.gene029018:intron | 65.0% | |
| AACAGCGGCGCAACCACCAC+CGG | - | chr2.3:11672842-11672861 | MS.gene029018:intron | 65.0% | |
| CCGGAGGAACTAGAAGCCGG+TGG | + | chr2.3:11672861-11672880 | None:intergenic | 65.0% | |
| ! | CGTCAAAGGCGGCGATTCGG+CGG | - | chr2.3:11673179-11673198 | MS.gene029018:CDS | 65.0% |
| ! | CTAGCGCCGGAGGCTTTCCA+CGG | + | chr2.3:11672612-11672631 | None:intergenic | 65.0% |
| !! | TTGTTGCTCGGCGCTGGCAC+CGG | + | chr2.3:11672709-11672728 | None:intergenic | 65.0% |
| CCCACGCCGTGGAAAGCCTC+CGG | - | chr2.3:11672603-11672622 | MS.gene029018:intron | 70.0% | |
| CCGGAGGCTTTCCACGGCGT+GGG | + | chr2.3:11672606-11672625 | None:intergenic | 70.0% | |
| CGCGTTACCGAGCCTAGCGC+CGG | + | chr2.3:11672625-11672644 | None:intergenic | 70.0% | |
| CGTGGAAAGCCTCCGGCGCT+AGG | - | chr2.3:11672610-11672629 | MS.gene029018:intron | 70.0% | |
| ! | GTTACCGAGCCTAGCGCCGG+AGG | + | chr2.3:11672622-11672641 | None:intergenic | 70.0% |
| GCCGGAGGCTTTCCACGGCG+TGG | + | chr2.3:11672607-11672626 | None:intergenic | 75.0% | |
| !!! | CTCGGCGCTGGCACCGGCGT+TGG | + | chr2.3:11672703-11672722 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 11672447 | 11673246 | 11672447 | ID=MS.gene029018 |
| chr2.3 | mRNA | 11672447 | 11673246 | 11672447 | ID=MS.gene029018.t1;Parent=MS.gene029018 |
| chr2.3 | exon | 11673057 | 11673246 | 11673057 | ID=MS.gene029018.t1.exon1;Parent=MS.gene029018.t1 |
| chr2.3 | CDS | 11673057 | 11673246 | 11673057 | ID=cds.MS.gene029018.t1;Parent=MS.gene029018.t1 |
| chr2.3 | exon | 11672701 | 11672846 | 11672701 | ID=MS.gene029018.t1.exon2;Parent=MS.gene029018.t1 |
| chr2.3 | CDS | 11672701 | 11672846 | 11672701 | ID=cds.MS.gene029018.t1;Parent=MS.gene029018.t1 |
| chr2.3 | exon | 11672447 | 11672593 | 11672447 | ID=MS.gene029018.t1.exon3;Parent=MS.gene029018.t1 |
| chr2.3 | CDS | 11672447 | 11672593 | 11672447 | ID=cds.MS.gene029018.t1;Parent=MS.gene029018.t1 |
| Gene Sequence |
| Protein sequence |
