AlfalfaGEDB | MS.gene02909

M. sativa cultivar XinJiangDaYe / MS.gene02909

KEGG: -
GO: -
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02909.t1	XP_013462948.1	86.8	379	49	1	1	379	1	378	8.70E-118	433.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02909.t1	Q39117	54.8	104	43	1	255	358	389	488	1.2e-27	125.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02909.t1	A0A072V6L9	86.8	379	49	1	1	379	1	378	6.3e-118	433.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene02909.t1	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02909.t1	MTR_2g026725	94.459	379	20	1	1	379	1	378	0.0	649
MS.gene02909.t1	MTR_1g098900	38.907	311	148	9	77	351	238	542	3.23e-53	186
MS.gene02909.t1	MTR_1g098900	41.379	87	50	1	263	349	57	142	2.46e-15	77.8
MS.gene02909.t1	MTR_1g098870	37.308	260	121	6	106	351	229	460	2.79e-43	158
MS.gene02909.t1	MTR_1g098870	42.529	87	49	1	263	349	55	140	4.34e-15	77.0
MS.gene02909.t1	MTR_1g081180	31.741	293	176	6	105	373	238	530	5.48e-35	135
MS.gene02909.t1	MTR_6g035370	60.870	92	36	0	260	351	461	552	3.80e-33	130
MS.gene02909.t1	MTR_6g035370	38.298	94	57	1	262	355	54	146	1.10e-15	79.0
MS.gene02909.t1	MTR_7g114860	31.395	258	135	8	115	350	240	477	3.88e-29	118
MS.gene02909.t1	MTR_1g492790	27.049	244	129	7	96	332	283	484	2.63e-16	80.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02909.t1	AT5G47660	41.365	249	121	3	106	354	169	392	3.11e-51	176
MS.gene02909.t1	AT1G76890	38.168	262	128	6	103	354	253	490	2.18e-44	161
MS.gene02909.t1	AT1G76890	41.176	85	49	1	263	347	41	124	3.30e-14	74.3
MS.gene02909.t1	AT5G28300	35.361	263	138	8	115	350	299	556	4.76e-37	142
MS.gene02909.t1	AT1G76880	53.398	103	44	1	256	358	401	499	6.24e-31	124
MS.gene02909.t1	AT1G76880	41.379	87	50	1	263	349	61	146	2.31e-14	75.1
MS.gene02909.t1	AT1G33240	54.839	93	42	0	263	355	435	527	6.09e-29	118
MS.gene02909.t1	AT1G33240	39.080	87	52	1	263	349	62	147	2.58e-13	71.6
MS.gene02909.t1	AT1G33240	54.839	93	42	0	263	355	435	527	7.91e-29	118
MS.gene02909.t1	AT1G33240	39.080	87	52	1	263	349	62	147	2.51e-13	72.0

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Find 139 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATCAATAAGTACTAT+AGG	0.424007	2.4:-62021978	MS.gene02909:CDS
AAATGGAGCAACATGGTCGG+AGG	0.635563	2.4:+62023816	None:intergenic
AAATTTGGTAAAGAGGATGA+CGG	0.659081	2.4:-62022568	MS.gene02909:CDS
AAGAAGCTAGAGCGGCTGAG+AGG	0.648990	2.4:-62022428	MS.gene02909:CDS
AAGAGAAGAGGATGAAGTTG+AGG	0.502881	2.4:-62022319	MS.gene02909:CDS
AAGATAAGGTCGCCGGAGTC+GGG	0.457626	2.4:+62023793	None:intergenic
AAGTACTATAGGAGGACAGT+TGG	0.558326	2.4:-62021967	MS.gene02909:CDS
AATGATTGAGAGGATGGAAA+AGG	0.532492	2.4:-62022511	MS.gene02909:CDS
AATGGGTCCTTGGAGAGAAA+TGG	0.436187	2.4:-62021874	MS.gene02909:CDS
AATTCAGCTCAAGAAAGAGA+AGG	0.601809	2.4:-62022626	MS.gene02909:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTTGGTAAAGAGGATGA+CGG	-	chr2.4:62023080-62023099	MS.gene02909:intron	30.0%
ACATCAATAAGTACTATAGG+AGG	-	chr2.4:62023673-62023692	MS.gene02909:CDS	30.0%
AGTCAAACAATTCAAACTGT+TGG	-	chr2.4:62022514-62022533	MS.gene02909:CDS	30.0%
ATACCAACAATGTTTCCATA+AGG	-	chr2.4:62023811-62023830	MS.gene02909:CDS	30.0%
TACCAACAATGTTTCCATAA+GGG	-	chr2.4:62023812-62023831	MS.gene02909:CDS	30.0%
TGGACAATCTGTATAGAAAT+GGG	-	chr2.4:62023757-62023776	MS.gene02909:CDS	30.0%
TGGATAAAGGATATATCGAA+CGG	-	chr2.4:62022861-62022880	MS.gene02909:intron	30.0%
TGTGTTACAAACATGCAAAT+TGG	-	chr2.4:62022365-62022384	MS.gene02909:CDS	30.0%
TGTTTGACTAACATAAATGC+CGG	+	chr2.4:62022503-62022522	None:intergenic	30.0%
TTGGACAATCTGTATAGAAA+TGG	-	chr2.4:62023756-62023775	MS.gene02909:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	62021810	62023860	62021810	ID=MS.gene02909
chr2.4	mRNA	62021810	62023860	62021810	ID=MS.gene02909.t1;Parent=MS.gene02909
chr2.4	exon	62023604	62023860	62023604	ID=MS.gene02909.t1.exon1;Parent=MS.gene02909.t1
chr2.4	CDS	62023604	62023860	62023604	ID=cds.MS.gene02909.t1;Parent=MS.gene02909.t1
chr2.4	exon	62021810	62022692	62021810	ID=MS.gene02909.t1.exon2;Parent=MS.gene02909.t1
chr2.4	CDS	62021810	62022692	62021810	ID=cds.MS.gene02909.t1;Parent=MS.gene02909.t1

Gene Sequence

>MS.gene02909

ATGGACAGCATGAACCTCCTCGCCTCCGACCATGTTGCTCCATTTCCCGACTCCGGCGACCTTATCTTCGCCGCCGGCCTCCTCTCCAACCACCACAATCCCCAAAAACTCATCCCCATCAGGTCCATCCCTCCTCCTCCTCCTCCTCCTCCTGGTTTCCCTTCTCACCACACCCCTCTACCACCTTCCATAAAGGTCGAGGACCCGTCTCGATCCGGGTCGGGTCATTCTTCTTTGCCCCTTTGTAAAGAACCCGGGTAAGTTCTTCTTTTATCCCTTAGCTTGTAAAGTTTTTACTTTTAAATTCATAAAGTTTTTATCTTTTTATCTTTTTTATTTTGGTTGAAAATTCAATGGTTAAACAATTAGGTTTAGTTTTAGTTCTCTCTGTTATTTTTTCAATTTTTTTTTTTTAATTCAATTTGAATTTGTTATGTTTGTTCAATTAAGCACGTTTCAAAAAAGCTAGAAAAAAGTGATTTTTAGCAGATAATTGTGTTTATTTTACTGTATCTTGATGAGATAAGAGTGGATAAATTTTGTCAATTTTCATGTTGTGTTACAAACATGCAAATTGGTTACAGTACTTTGTATGATGGTAAATGTTGTTTTATGGTGGTGTGTTAGTGAAGAAAAAAGAATCATACAAGGTATTGTAGTTTGGATTTGCATTTTCGATCGTTGTGGAATCCGGCATTTATGTTAGTCAAACAATTCAAACTGTTGGATCAATATTAAATGGTTTGAGGGTTTAGATTGATTTGACTGCAGTAAATGTATAATTTGTAGACTTTCCTGATGGCGGGGGTTTCAGATCTAACTAGCTATGTTTTATTTGCAGGATCCAAGTTTTTATGTAGCATATATAATAGTTCATTAGGTAATAAATCCAGTGTTGTCGAATAGCAGCGTTGAATTTGAATAAATCACTATTCTTCTGCTATTTAGTAAAGGGTCGTCAAATAGTCGCTATTGCATGGTAGTGTAGTTGAATTTGACTAAACTGTTCTTTTCTGCTATGTGCTATTGACAACATTGGATAAATCTACTTTGGATAAAGGATATATCGAACGGAACTATTATCCATTTATTTTGGGGTTAAACATAAGGTTTGATGGAATATTTCCTTTTACGATAATGATTCGAATTTGTGTTTTCATCATTTCAGCTCATCTTCGGATGACGTGGGCAATTCATCTGCTAGAACTAAAGAATTCAGCTCAAGAAAGAGAAGGAGAAAGAGTTCGAGAAAGTTGGAAGATTTTGCAGCAAATTTGGTAAAGAGGATGACGGAAAAGCAAGAGCAGATGCATAAAGAATTGGTGGAAATGATTGAGAGGATGGAAAAGGAAAGGATAAAGAGAGAAGAAGCATGGAAGCGCGAGGAGATGGAGAGAATTAAGCAGGACGAAGAAGCTAGAGCGGCTGAGAGGTCTCGTAGTTTAGCCCTCATATCGCTGATTCAGAAACTATTGGGTTATGAAATTCAGATTCCTCCACCAGCAGAAGGAAGCGGAATAAGAGAAGAGGATGAAGTTGAGGTGAACAACAACAACAACAGCCCACCCTTGACCCACAACGTGGGGTCGGCTGAGGTGAACAACAACACCAACAGCCTAGCCTTAATCCAGTACATGGGGCCGGCTGAGGTGAGCAATAACAAAAAACATTTCAACAGCGGCGATCCAAGTAACAGTAGATGGCCAGATGTTGAAGTACAAGCGCTCATAGCAGTAAGAACCGCATTGGAACACAAGTTCGGTCATATGGGATCGAAAGGATCAATATGGGAGGAGATAGCTGGAGCATTGCAGGGCATGGGATACACTCGTTCTGCGAAGAAGTGCAAGGAGAAATGGGAAAACATCAATAAGTACTATAGGAGGACAGTTGGGAGCGGCAAGAAACGGCCTGTTAATAGCAAAACTTGCCCTTACTTTGACGAATTGGACAATCTGTATAGAAATGGGTCCTTGGAGAGAAATGGAAATGCATTGAGCAATACCAACAATGTTTCCATAAGGGAAGAAAAGGAGCAGGGTGAAATTTGA

Protein sequence

>MS.gene02909.t1

MDSMNLLASDHVAPFPDSGDLIFAAGLLSNHHNPQKLIPIRSIPPPPPPPPGFPSHHTPLPPSIKVEDPSRSGSGHSSLPLCKEPGSSSDDVGNSSARTKEFSSRKRRRKSSRKLEDFAANLVKRMTEKQEQMHKELVEMIERMEKERIKREEAWKREEMERIKQDEEARAAERSRSLALISLIQKLLGYEIQIPPPAEGSGIREEDEVEVNNNNNSPPLTHNVGSAEVNNNTNSLALIQYMGPAEVSNNKKHFNSGDPSNSRWPDVEVQALIAVRTALEHKFGHMGSKGSIWEEIAGALQGMGYTRSAKKCKEKWENINKYYRRTVGSGKKRPVNSKTCPYFDELDNLYRNGSLERNGNALSNTNNVSIREEKEQGEI