AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02916

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02916.t1 MTR_3g450850 69.004 271 83 1 31 300 16 286 9.45e-131 389
MS.gene02916.t1 MTR_4g035820 37.421 318 175 6 60 370 74 374 1.63e-51 189
MS.gene02916.t1 MTR_4g035820 29.327 208 100 5 432 624 327 502 9.27e-13 72.0
MS.gene02916.t1 MTR_3g012160 83.696 92 15 0 210 301 2 93 1.00e-46 160
MS.gene02916.t1 MTR_4g071400 96.203 79 3 0 545 623 43 121 1.27e-43 152
MS.gene02916.t1 MTR_1g070355 32.143 252 151 7 61 306 72 309 3.41e-28 120
MS.gene02916.t1 MTR_6g051425 27.562 283 193 5 62 341 18 291 1.30e-27 114
MS.gene02916.t1 MTR_5g076070 27.500 240 158 7 64 300 56 282 7.47e-23 99.8
MS.gene02916.t1 MTR_3g095940 74.074 54 14 0 485 538 90 143 3.59e-20 87.8
MS.gene02916.t1 MTR_5g083630 57.143 70 24 1 204 267 7 76 5.97e-18 80.5
MS.gene02916.t1 MTR_6g014355 36.364 154 82 5 142 287 14 159 4.96e-16 78.6
MS.gene02916.t1 MTR_0558s0020 24.715 263 175 7 66 312 41 296 1.26e-13 72.4
MS.gene02916.t1 MTR_6g022080 30.303 165 98 6 72 232 96 247 1.69e-13 71.2
MS.gene02916.t1 MTR_8g446020 30.667 150 96 3 167 312 111 256 1.79e-12 68.6
MS.gene02916.t1 MTR_1g019850 30.000 140 80 3 67 205 76 198 6.53e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02916.t1 AT1G10240 45.636 550 256 9 30 543 11 553 1.41e-163 486
MS.gene02916.t1 AT1G10240 45.636 550 256 9 30 543 11 553 1.41e-163 486
MS.gene02916.t1 AT5G28530 41.058 548 277 5 31 541 27 565 4.25e-148 446
MS.gene02916.t1 AT5G28530 41.176 544 278 5 31 541 27 561 6.89e-148 445
MS.gene02916.t1 AT5G28530 41.176 544 278 5 31 541 27 561 6.89e-148 445
MS.gene02916.t1 AT5G28530 33.761 545 258 8 31 541 27 502 2.75e-93 301
MS.gene02916.t1 AT5G28530 33.826 541 259 8 31 541 27 498 4.04e-93 301
MS.gene02916.t1 AT4G38180 29.273 509 317 10 61 537 73 570 1.79e-58 211
MS.gene02916.t1 AT4G15090 28.571 490 299 13 61 515 51 524 1.38e-55 203
MS.gene02916.t1 AT4G15090 28.571 490 299 13 61 515 51 524 1.38e-55 203
MS.gene02916.t1 AT4G15090 28.571 490 299 13 61 515 51 524 1.38e-55 203
MS.gene02916.t1 AT4G15090 28.571 490 299 13 61 515 51 524 1.38e-55 203
MS.gene02916.t1 AT4G15090 28.571 490 299 13 61 515 51 524 1.38e-55 203
MS.gene02916.t1 AT3G22170 25.490 510 306 17 49 515 55 533 8.70e-44 169
MS.gene02916.t1 AT3G22170 25.490 510 306 17 49 515 55 533 8.70e-44 169
MS.gene02916.t1 AT1G76320 25.615 488 295 14 67 515 3 461 1.71e-39 155
MS.gene02916.t1 AT1G76320 25.615 488 295 14 67 515 3 461 1.73e-39 155
MS.gene02916.t1 AT1G76320 25.615 488 295 14 67 515 3 461 1.73e-39 155
MS.gene02916.t1 AT1G76320 25.615 488 295 14 67 515 3 461 1.83e-39 155
MS.gene02916.t1 AT3G06250 25.800 500 277 16 61 526 190 629 5.59e-39 154
MS.gene02916.t1 AT3G06250 25.800 500 277 16 61 526 190 629 5.59e-39 154
MS.gene02916.t1 AT3G06250 25.800 500 277 16 61 526 190 629 5.59e-39 154
MS.gene02916.t1 AT5G18960 24.565 517 293 14 47 526 197 653 9.53e-37 147
MS.gene02916.t1 AT5G18960 24.565 517 293 14 47 526 197 653 9.53e-37 147
MS.gene02916.t1 AT2G27110 24.431 483 299 14 61 515 49 493 5.05e-33 136
MS.gene02916.t1 AT2G27110 24.431 483 299 14 61 515 49 493 5.05e-33 136
MS.gene02916.t1 AT2G32250 25.862 290 184 5 256 515 180 468 9.96e-30 125
MS.gene02916.t1 AT2G32250 25.862 290 184 5 256 515 180 468 9.96e-30 125
MS.gene02916.t1 AT2G32250 25.862 290 184 5 256 515 180 468 1.03e-29 125
MS.gene02916.t1 AT2G32250 25.862 290 184 5 256 515 180 468 1.15e-29 125
MS.gene02916.t1 AT2G32250 25.862 290 184 5 256 515 180 468 1.15e-29 125
MS.gene02916.t1 AT2G32250 25.862 290 184 5 256 515 180 468 1.15e-29 125
MS.gene02916.t1 AT2G32250 25.862 290 184 5 256 515 139 427 1.20e-29 125
MS.gene02916.t1 AT2G32250 25.862 290 184 5 256 515 139 427 1.25e-29 125
MS.gene02916.t1 AT2G27110 26.645 304 192 7 240 515 48 348 1.28e-29 125
MS.gene02916.t1 AT4G19990 26.243 503 268 18 63 515 37 486 1.68e-28 122
MS.gene02916.t1 AT4G19990 26.243 503 268 18 63 515 23 472 1.70e-28 122
MS.gene02916.t1 AT4G19990 26.243 503 268 18 63 515 23 472 1.70e-28 122
MS.gene02916.t1 AT4G19990 26.243 503 268 18 63 515 23 472 1.84e-28 122
MS.gene02916.t1 AT4G19990 26.243 503 268 18 63 515 23 472 2.17e-28 121
MS.gene02916.t1 AT4G19990 26.243 503 268 18 63 515 23 472 2.17e-28 121
MS.gene02916.t1 AT1G52520 24.746 493 303 16 61 516 85 546 9.93e-28 119
MS.gene02916.t1 AT1G80010 22.993 548 300 19 61 534 95 594 1.86e-25 112
MS.gene02916.t1 AT4G19990 25.612 449 247 15 112 515 9 415 4.13e-24 108
MS.gene02916.t1 AT4G38170 26.301 346 215 9 244 554 16 356 1.29e-23 105

Find 112 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTAAAACTTACTTATT+AGG 0.177601 2.4:-62099203 None:intergenic
ATGAGATGCCTCTTGGAATA+TGG 0.196486 2.4:+62097671 MS.gene02916:CDS
TTCTCACAACCACGAGTTAT+TGG 0.199264 2.4:+62097249 MS.gene02916:CDS
ATCACATGTTCTTGAGTTTC+AGG 0.210618 2.4:-62098960 None:intergenic
CTCATGTTGAAGCAACTTAA+AGG 0.248230 2.4:-62098526 None:intergenic
TGTTTATTTGCAAAACAAAA+TGG 0.260407 2.4:+62096983 MS.gene02916:CDS
ATTCGTGCATATGAAGCTTT+TGG 0.265394 2.4:+62097607 MS.gene02916:CDS
CAATATGAATTCACAATTGA+TGG 0.266214 2.4:+62097541 MS.gene02916:CDS
TTCCTTCGCATGGGCTATAA+AGG 0.299718 2.4:+62097762 MS.gene02916:CDS
AATATCATCATAAAGATAAA+AGG 0.310779 2.4:+62098933 MS.gene02916:CDS
TCAAGAACATGTGATTGATT+TGG 0.314506 2.4:+62098968 MS.gene02916:CDS
CATCAATTGTGAATTCATAT+TGG 0.326055 2.4:-62097540 None:intergenic
GCAGCTTCAACCCCTCCTTT+TGG 0.339248 2.4:-62099074 None:intergenic
ATTCAATCATATAGTGGTAT+TGG 0.341059 2.4:+62097451 MS.gene02916:CDS
ACTCTCGCTATAAGACTTAC+AGG 0.343612 2.4:-62097361 None:intergenic
CAAATTGGAGCATATTGTTT+GGG 0.347369 2.4:+62097570 MS.gene02916:CDS
GAGCATATTGTTTGGGCATT+TGG 0.348974 2.4:+62097577 MS.gene02916:CDS
ACAAATTGGAGCATATTGTT+TGG 0.352856 2.4:+62097569 MS.gene02916:CDS
TTAGTCTCTAATTCGATGTT+TGG 0.356770 2.4:+62098854 MS.gene02916:intron
AATTGATGGAAGTAACAAAT+TGG 0.358783 2.4:+62097555 MS.gene02916:CDS
ATGACAACGACGAGGCTTCT+TGG 0.366012 2.4:-62099096 None:intergenic
AACGTCACAGTCAGCATTGT+TGG 0.377007 2.4:-62099003 None:intergenic
ATGGTCTCACAGTTTGGTTT+GGG 0.387072 2.4:+62098126 MS.gene02916:CDS
GTAGTTACTAAGAGAAGAAT+TGG 0.389295 2.4:+62097190 MS.gene02916:CDS
AATGGTCTCACAGTTTGGTT+TGG 0.398201 2.4:+62098125 MS.gene02916:CDS
CCTCAAGTCACTAACATTGA+TGG 0.399508 2.4:+62096794 MS.gene02916:CDS
GCAATGGCTTCTTTAATTGA+AGG 0.406562 2.4:-62097934 None:intergenic
TCAAATATTTGCCCTTCAAA+AGG 0.411166 2.4:-62096824 None:intergenic
ATCCATCGGGTGTTTATAAG+AGG 0.417116 2.4:+62097062 MS.gene02916:CDS
TTTGCTGGAATGACAACAAC+AGG 0.418779 2.4:+62098222 MS.gene02916:CDS
AAAGTAATGGTCTCACAGTT+TGG 0.427267 2.4:+62098120 MS.gene02916:CDS
TGAGATGCCTCTTGGAATAT+GGG 0.433904 2.4:+62097672 MS.gene02916:CDS
TTGGGCCTTAGCTTATTTGA+AGG 0.435274 2.4:+62098191 MS.gene02916:intron
TATACGACCACCATCAACTT+TGG 0.437731 2.4:-62098616 None:intergenic
CTTTAAACATGAGTTTCATA+AGG 0.439926 2.4:+62098056 MS.gene02916:CDS
GACGAGGCATGTTCTTCAAT+AGG 0.443666 2.4:-62098486 None:intergenic
TGATTAGTTCCACCACAATC+TGG 0.446933 2.4:-62099122 None:intergenic
AACCGTTATGAGATGCCTCT+TGG 0.448749 2.4:+62097664 MS.gene02916:CDS
AAAGACAAGTGAACGAGAAC+AGG 0.453486 2.4:+62098890 MS.gene02916:CDS
GGAAAATGTGTTGTAATATG+AGG 0.458305 2.4:-62098465 None:intergenic
GTTTGTCATCGTGGTGGTAT+TGG 0.458689 2.4:+62097091 MS.gene02916:CDS
ATGCGATCTTGATCAACAAT+TGG 0.465554 2.4:-62097313 None:intergenic
ATAACGGTTTATTCTATATG+TGG 0.472493 2.4:-62097650 None:intergenic
TATAAGACTTACAGGGCAAC+CGG 0.483233 2.4:-62097353 None:intergenic
CTTGAGTTAGAGAAAAGAAC+AGG 0.494786 2.4:+62097385 MS.gene02916:CDS
AAAACAAAAGGTCGGCCAAA+AGG 0.495104 2.4:+62099044 MS.gene02916:CDS
TTGATAGGATCATGAAGGGT+TGG 0.498564 2.4:+62099290 MS.gene02916:intron
TGAAGCAACTTAAAGGCATA+AGG 0.499495 2.4:-62098519 None:intergenic
AGAAGATTTCTTCCTTCGCA+TGG 0.503824 2.4:+62097752 MS.gene02916:CDS
CGTTGTCATGTTCCAGATTG+TGG 0.504732 2.4:+62099110 MS.gene02916:CDS
AATTGTGAATTCATATTGGA+AGG 0.507660 2.4:-62097536 None:intergenic
CCAAAAGGATCAAGACCAAA+AGG 0.514192 2.4:+62099059 MS.gene02916:CDS
GTCAAAATTAGAAATCAAGC+TGG 0.517568 2.4:+62098408 MS.gene02916:CDS
TTTAATTGAAGGATCTTGAT+CGG 0.518039 2.4:-62097923 None:intergenic
ATTGGAATGTTACGATAAGC+AGG 0.522557 2.4:-62097295 None:intergenic
ATGTTATTGACTTGAGGCAA+GGG 0.525418 2.4:-62096896 None:intergenic
TAAGCAATGATGCTAGTGAT+TGG 0.525487 2.4:+62096765 MS.gene02916:CDS
ACCACGCAAAACTGTTGAAG+TGG 0.526679 2.4:+62097117 MS.gene02916:CDS
TTAGAGAAAAGAACAGGCAC+AGG 0.528687 2.4:+62097391 MS.gene02916:CDS
TGGGTTGGTGTTGATAATCA+TGG 0.529258 2.4:+62097691 MS.gene02916:CDS
ATACTATTATTGTCTAAAGC+CGG 0.531055 2.4:+62097334 MS.gene02916:CDS
TTCCAAGAGGCATCTCATAA+CGG 0.531646 2.4:-62097666 None:intergenic
CAACATCATACCAAAGTTGA+TGG 0.538288 2.4:+62098606 MS.gene02916:CDS
TGAACGAGAATGGAAAGTAA+TGG 0.544871 2.4:+62098107 MS.gene02916:CDS
TTGCACAACATAAGAGCCAT+TGG 0.551354 2.4:-62098586 None:intergenic
CTCAAGTCACTAACATTGAT+GGG 0.551567 2.4:+62096795 MS.gene02916:CDS
TAAGAGAAGAATTGGCATTG+AGG 0.553953 2.4:+62097198 MS.gene02916:CDS
GAAGATTTCTTCCTTCGCAT+GGG 0.556681 2.4:+62097753 MS.gene02916:CDS
GTGCTTTGATAGGATCATGA+AGG 0.556862 2.4:+62099285 MS.gene02916:intron
CAAGTCAATAACATTGATGA+GGG 0.558520 2.4:+62096905 MS.gene02916:CDS
ACATGTGATTGATTTGGAAG+AGG 0.565753 2.4:+62098974 MS.gene02916:CDS
GTTTGGCAATTCCATTGCAA+TGG 0.567761 2.4:-62097950 None:intergenic
ATGTGATTGTAGTGCAAAGA+TGG 0.570079 2.4:+62097168 MS.gene02916:CDS
ATGTTAGTGACTTGAGGCAA+GGG 0.572230 2.4:-62096788 None:intergenic
GTGTCAGAAGAGAATCCATC+GGG 0.572540 2.4:+62097049 MS.gene02916:CDS
TCATCAATGTTATTGACTTG+AGG 0.573525 2.4:-62096902 None:intergenic
AATTAAAGAAGCCATTGCAA+TGG 0.576797 2.4:+62097939 MS.gene02916:CDS
TAGAAATCAAGCTGGAGAAG+AGG 0.577533 2.4:+62098416 MS.gene02916:CDS
CGAGTTATTGGATGACAAAG+AGG 0.580002 2.4:+62097261 MS.gene02916:CDS
AATGTTATTGACTTGAGGCA+AGG 0.580693 2.4:-62096897 None:intergenic
AGTCACTAACATTGATGGGT+GGG 0.589348 2.4:+62096799 MS.gene02916:CDS
TTGTCGCAAAGCTGTCAAGT+TGG 0.589381 2.4:+62098001 MS.gene02916:CDS
TCATCATATCTCGAACCTAG+TGG 0.597376 2.4:-62098033 None:intergenic
AATGTTAGTGACTTGAGGCA+AGG 0.597462 2.4:-62096789 None:intergenic
AGGGAATTTGTTTGTCATCG+TGG 0.598966 2.4:+62097082 MS.gene02916:CDS
TCCCTCTTATAAACACCCGA+TGG 0.599604 2.4:-62097064 None:intergenic
GCAGCAATTGAAGCTGCCAA+TGG 0.600590 2.4:+62098570 MS.gene02916:CDS
TACCTTTATAGCCCATGCGA+AGG 0.601051 2.4:-62097764 None:intergenic
TCAAGTCAATAACATTGATG+AGG 0.603097 2.4:+62096904 MS.gene02916:CDS
CTGTTGAAGTGGAAAATCAA+AGG 0.606328 2.4:+62097128 MS.gene02916:CDS
CTTTGTCATCCAATAACTCG+TGG 0.606716 2.4:-62097258 None:intergenic
AAGGATCAAGACCAAAAGGA+GGG 0.611165 2.4:+62099063 MS.gene02916:CDS
CCATCAATGTTAGTGACTTG+AGG 0.613145 2.4:-62096794 None:intergenic
ATGCCTCTTGGAATATGGGT+TGG 0.616141 2.4:+62097676 MS.gene02916:CDS
GAATTTGTTTGTCATCGTGG+TGG 0.618777 2.4:+62097085 MS.gene02916:CDS
AAGTCACTAACATTGATGGG+TGG 0.624878 2.4:+62096798 MS.gene02916:CDS
TCCATCGGGTGTTTATAAGA+GGG 0.625908 2.4:+62097063 MS.gene02916:CDS
ACGAGAACAGGTTGCCACAA+GGG 0.630576 2.4:+62098902 MS.gene02916:CDS
TGTGTCAGAAGAGAATCCAT+CGG 0.632507 2.4:+62097048 MS.gene02916:CDS
AACGAGAACAGGTTGCCACA+AGG 0.633734 2.4:+62098901 MS.gene02916:CDS
TACTTATTAGGAGATTGAGA+AGG 0.635742 2.4:-62099191 None:intergenic
TGTCATGTTCCAGATTGTGG+TGG 0.637809 2.4:+62099113 MS.gene02916:CDS
CTCTCGCTATAAGACTTACA+GGG 0.647869 2.4:-62097360 None:intergenic
AAAGGATCAAGACCAAAAGG+AGG 0.655085 2.4:+62099062 MS.gene02916:CDS
ACACCAACCCATATTCCAAG+AGG 0.676376 2.4:-62097679 None:intergenic
TGCTTTGATAGGATCATGAA+GGG 0.676443 2.4:+62099286 MS.gene02916:intron
AAGTTTGAAAGATCGAGATG+AGG 0.682349 2.4:+62097513 MS.gene02916:CDS
CATCATACCAAAGTTGATGG+TGG 0.683172 2.4:+62098609 MS.gene02916:CDS
AGGATCAAGACCAAAAGGAG+GGG 0.696841 2.4:+62099064 MS.gene02916:CDS
AAGTCAATAACATTGATGAG+GGG 0.714581 2.4:+62096906 MS.gene02916:CDS
ATAAGGTGTGAGTATAGACG+AGG 0.720548 2.4:-62098502 None:intergenic
TCTGGAACATGACAACGACG+AGG 0.797744 2.4:-62099104 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATTAACTGTATTTTTA+TGG + chr2.4:62099263-62099282 MS.gene02916:intron 10.0%
!! AATATCATCATAAAGATAAA+AGG + chr2.4:62098933-62098952 MS.gene02916:CDS 15.0%
!!! AATTTTATTCAATCATATAG+TGG + chr2.4:62097445-62097464 MS.gene02916:CDS 15.0%
!! CCAATTAAAACTTACTTATT+AGG - chr2.4:62099206-62099225 None:intergenic 20.0%
!! TGTTTATTTGCAAAACAAAA+TGG + chr2.4:62096983-62097002 MS.gene02916:CDS 20.0%
!!! AACAAAATGGATTTTCTATT+AGG + chr2.4:62096996-62097015 MS.gene02916:CDS 20.0%
!!! CATGGAAATTCAATTTTTTT+TGG + chr2.4:62097709-62097728 MS.gene02916:CDS 20.0%
!!! CCTAATAAGTAAGTTTTAAT+TGG + chr2.4:62099203-62099222 MS.gene02916:intron 20.0%
!!! CTAATATCTTTTTCTAAGAA+TGG - chr2.4:62097424-62097443 None:intergenic 20.0%
!!! TGATTGTTATATTTTTGTGT+AGG + chr2.4:62098374-62098393 MS.gene02916:intron 20.0%
!!! TTATTACATGACTAGATTTT+GGG - chr2.4:62098806-62098825 None:intergenic 20.0%
!!! TTTATTACATGACTAGATTT+TGG - chr2.4:62098807-62098826 None:intergenic 20.0%
! AATTGATGGAAGTAACAAAT+TGG + chr2.4:62097555-62097574 MS.gene02916:CDS 25.0%
! AATTGTGAATTCATATTGGA+AGG - chr2.4:62097539-62097558 None:intergenic 25.0%
! ATAACGGTTTATTCTATATG+TGG - chr2.4:62097653-62097672 None:intergenic 25.0%
! ATTCAATCATATAGTGGTAT+TGG + chr2.4:62097451-62097470 MS.gene02916:CDS 25.0%
! CAATATGAATTCACAATTGA+TGG + chr2.4:62097541-62097560 MS.gene02916:CDS 25.0%
! CATCAATTGTGAATTCATAT+TGG - chr2.4:62097543-62097562 None:intergenic 25.0%
! CTTTAAACATGAGTTTCATA+AGG + chr2.4:62098056-62098075 MS.gene02916:CDS 25.0%
! TCCAAACAAAAAGAGAAAAA+TGG + chr2.4:62099160-62099179 MS.gene02916:CDS 25.0%
! TTGTTTCAAAAACAAAAGGT+CGG + chr2.4:62099036-62099055 MS.gene02916:CDS 25.0%
! TTTAATTGAAGGATCTTGAT+CGG - chr2.4:62097926-62097945 None:intergenic 25.0%
!! ATACTATTATTGTCTAAAGC+CGG + chr2.4:62097334-62097353 MS.gene02916:CDS 25.0%
!! TCAAATATTTTCCCTTCAAA+AGG - chr2.4:62096935-62096954 None:intergenic 25.0%
!! TGTAAAGAGTTTCAATTTTC+AGG + chr2.4:62098678-62098697 MS.gene02916:intron 25.0%
!!! AAAATCTGTCAAACTTGTTT+TGG - chr2.4:62098292-62098311 None:intergenic 25.0%
!!! TATTTTTATGGTGCTTTGAT+AGG + chr2.4:62099275-62099294 MS.gene02916:intron 25.0%
!!! TCCATTTTTCTCTTTTTGTT+TGG - chr2.4:62099164-62099183 None:intergenic 25.0%
!!! TGTTATATTTTTGTGTAGGT+TGG + chr2.4:62098378-62098397 MS.gene02916:intron 25.0%
AAAGTCCTTCAAATAAGCTA+AGG - chr2.4:62098199-62098218 None:intergenic 30.0%
AAGTCAATAACATTGATGAG+GGG + chr2.4:62096906-62096925 MS.gene02916:CDS 30.0%
AATTAAAGAAGCCATTGCAA+TGG + chr2.4:62097939-62097958 MS.gene02916:CDS 30.0%
ACAAATTGGAGCATATTGTT+TGG + chr2.4:62097569-62097588 MS.gene02916:CDS 30.0%
CAAATTGGAGCATATTGTTT+GGG + chr2.4:62097570-62097589 MS.gene02916:CDS 30.0%
CAAGTCAATAACATTGATGA+GGG + chr2.4:62096905-62096924 MS.gene02916:CDS 30.0%
CATGTAATAAACTGCAATGA+TGG + chr2.4:62098816-62098835 MS.gene02916:intron 30.0%
GGAAAATGTGTTGTAATATG+AGG - chr2.4:62098468-62098487 None:intergenic 30.0%
GTAGTTACTAAGAGAAGAAT+TGG + chr2.4:62097190-62097209 MS.gene02916:CDS 30.0%
GTCAAAATTAGAAATCAAGC+TGG + chr2.4:62098408-62098427 MS.gene02916:CDS 30.0%
TACTTATTAGGAGATTGAGA+AGG - chr2.4:62099194-62099213 None:intergenic 30.0%
TAGAGACTAAAATTTAGCAG+AGG - chr2.4:62098844-62098863 None:intergenic 30.0%
TCAAATATTTGCCCTTCAAA+AGG - chr2.4:62096827-62096846 None:intergenic 30.0%
TCAAGAACATGTGATTGATT+TGG + chr2.4:62098968-62098987 MS.gene02916:CDS 30.0%
TCAAGTCAATAACATTGATG+AGG + chr2.4:62096904-62096923 MS.gene02916:CDS 30.0%
TTAGTCTCTAATTCGATGTT+TGG + chr2.4:62098854-62098873 MS.gene02916:intron 30.0%
! AATTGGCATTGAGGAAAAAT+GGG + chr2.4:62097207-62097226 MS.gene02916:CDS 30.0%
! ATTTTCCTTTCACAAAAGCT+AGG - chr2.4:62097885-62097904 None:intergenic 30.0%
! CAAAACATGCATTTTGCATA+TGG + chr2.4:62097974-62097993 MS.gene02916:CDS 30.0%
! CAAAATATCTTCCTTTTCGA+TGG + chr2.4:62098760-62098779 MS.gene02916:intron 30.0%
! CAAAATGCATGTTTTGTGTT+TGG - chr2.4:62097970-62097989 None:intergenic 30.0%
! CTTTACCCATCGTAAATTTT+GGG + chr2.4:62098173-62098192 MS.gene02916:intron 30.0%
! TCTTTACCCATCGTAAATTT+TGG + chr2.4:62098172-62098191 MS.gene02916:intron 30.0%
! TGATCGGTAAGAATTGTTTT+TGG - chr2.4:62097910-62097929 None:intergenic 30.0%
!! CCTGTTGTTTCAAAAACAAA+AGG + chr2.4:62099032-62099051 MS.gene02916:CDS 30.0%
!! CGAAAAGGAAGATATTTTGA+TGG - chr2.4:62098759-62098778 None:intergenic 30.0%
!! TCATCAATGTTATTGACTTG+AGG - chr2.4:62096905-62096924 None:intergenic 30.0%
!!! CCTTTTGTTTTTGAAACAAC+AGG - chr2.4:62099035-62099054 None:intergenic 30.0%
!!! TTGAAGGACTTTTTTTTTGC+TGG + chr2.4:62098207-62098226 MS.gene02916:CDS 30.0%
AAAGTAATGGTCTCACAGTT+TGG + chr2.4:62098120-62098139 MS.gene02916:CDS 35.0%
AAGTTTGAAAGATCGAGATG+AGG + chr2.4:62097513-62097532 MS.gene02916:CDS 35.0%
ATCACATGTTCTTGAGTTTC+AGG - chr2.4:62098963-62098982 None:intergenic 35.0%
ATGCGATCTTGATCAACAAT+TGG - chr2.4:62097316-62097335 None:intergenic 35.0%
ATGTGATTGTAGTGCAAAGA+TGG + chr2.4:62097168-62097187 MS.gene02916:CDS 35.0%
ATTGGAATGTTACGATAAGC+AGG - chr2.4:62097298-62097317 None:intergenic 35.0%
CAACATCATACCAAAGTTGA+TGG + chr2.4:62098606-62098625 MS.gene02916:CDS 35.0%
CTCAAGTCACTAACATTGAT+GGG + chr2.4:62096795-62096814 MS.gene02916:CDS 35.0%
CTCATGTTGAAGCAACTTAA+AGG - chr2.4:62098529-62098548 None:intergenic 35.0%
CTGTTGAAGTGGAAAATCAA+AGG + chr2.4:62097128-62097147 MS.gene02916:CDS 35.0%
CTTGAGTTAGAGAAAAGAAC+AGG + chr2.4:62097385-62097404 MS.gene02916:CDS 35.0%
GCAATGGCTTCTTTAATTGA+AGG - chr2.4:62097937-62097956 None:intergenic 35.0%
TAAGAGAAGAATTGGCATTG+AGG + chr2.4:62097198-62097217 MS.gene02916:CDS 35.0%
TAAGCAATGATGCTAGTGAT+TGG + chr2.4:62096765-62096784 MS.gene02916:CDS 35.0%
TAAGGCCCAAAATTTACGAT+GGG - chr2.4:62098181-62098200 None:intergenic 35.0%
TGAACGAGAATGGAAAGTAA+TGG + chr2.4:62098107-62098126 MS.gene02916:CDS 35.0%
TGCTTTGATAGGATCATGAA+GGG + chr2.4:62099286-62099305 MS.gene02916:intron 35.0%
! ATTCGTGCATATGAAGCTTT+TGG + chr2.4:62097607-62097626 MS.gene02916:CDS 35.0%
! CAGATGATTTTGAACGAGAA+TGG + chr2.4:62098097-62098116 MS.gene02916:CDS 35.0%
! GAATTGGCATTGAGGAAAAA+TGG + chr2.4:62097206-62097225 MS.gene02916:CDS 35.0%
! GATATTTTCTAACTCCCTTG+TGG - chr2.4:62098919-62098938 None:intergenic 35.0%
! TGAAGCAACTTAAAGGCATA+AGG - chr2.4:62098522-62098541 None:intergenic 35.0%
!! AATGTTATTGACTTGAGGCA+AGG - chr2.4:62096900-62096919 None:intergenic 35.0%
!! ACATGTGATTGATTTGGAAG+AGG + chr2.4:62098974-62098993 MS.gene02916:CDS 35.0%
!! AGTTTCCTAGCTTTTGTGAA+AGG + chr2.4:62097877-62097896 MS.gene02916:CDS 35.0%
!! ATGTTATTGACTTGAGGCAA+GGG - chr2.4:62096899-62096918 None:intergenic 35.0%
!!! AGTTGGTTTTCTTTTCCACT+AGG + chr2.4:62098018-62098037 MS.gene02916:CDS 35.0%
AAAACAAAAGGTCGGCCAAA+AGG + chr2.4:62099044-62099063 MS.gene02916:CDS 40.0%
AAAGACAAGTGAACGAGAAC+AGG + chr2.4:62098890-62098909 MS.gene02916:CDS 40.0%
AAAGGATCAAGACCAAAAGG+AGG + chr2.4:62099062-62099081 MS.gene02916:CDS 40.0%
AAGGATCAAGACCAAAAGGA+GGG + chr2.4:62099063-62099082 MS.gene02916:CDS 40.0%
AAGTCACTAACATTGATGGG+TGG + chr2.4:62096798-62096817 MS.gene02916:CDS 40.0%
AATGGTCTCACAGTTTGGTT+TGG + chr2.4:62098125-62098144 MS.gene02916:CDS 40.0%
AATGTTAGTGACTTGAGGCA+AGG - chr2.4:62096792-62096811 None:intergenic 40.0%
ACTCTCGCTATAAGACTTAC+AGG - chr2.4:62097364-62097383 None:intergenic 40.0%
AGAAGATTTCTTCCTTCGCA+TGG + chr2.4:62097752-62097771 MS.gene02916:CDS 40.0%
ATAAGGTGTGAGTATAGACG+AGG - chr2.4:62098505-62098524 None:intergenic 40.0%
ATCCATCGGGTGTTTATAAG+AGG + chr2.4:62097062-62097081 MS.gene02916:CDS 40.0%
ATGAGATGCCTCTTGGAATA+TGG + chr2.4:62097671-62097690 MS.gene02916:CDS 40.0%
ATGTTAGTGACTTGAGGCAA+GGG - chr2.4:62096791-62096810 None:intergenic 40.0%
CCAAAAGGATCAAGACCAAA+AGG + chr2.4:62099059-62099078 MS.gene02916:CDS 40.0%
CCATCAATGTTAGTGACTTG+AGG - chr2.4:62096797-62096816 None:intergenic 40.0%
CCTCAAGTCACTAACATTGA+TGG + chr2.4:62096794-62096813 MS.gene02916:CDS 40.0%
CGAGTTATTGGATGACAAAG+AGG + chr2.4:62097261-62097280 MS.gene02916:CDS 40.0%
CTAAGGCCCAAAATTTACGA+TGG - chr2.4:62098182-62098201 None:intergenic 40.0%
CTCTCGCTATAAGACTTACA+GGG - chr2.4:62097363-62097382 None:intergenic 40.0%
CTTTGTCATCCAATAACTCG+TGG - chr2.4:62097261-62097280 None:intergenic 40.0%
GAAGATTTCTTCCTTCGCAT+GGG + chr2.4:62097753-62097772 MS.gene02916:CDS 40.0%
GAGCATATTGTTTGGGCATT+TGG + chr2.4:62097577-62097596 MS.gene02916:CDS 40.0%
GTGCTTTGATAGGATCATGA+AGG + chr2.4:62099285-62099304 MS.gene02916:intron 40.0%
GTTTGGCAATTCCATTGCAA+TGG - chr2.4:62097953-62097972 None:intergenic 40.0%
TAGAAATCAAGCTGGAGAAG+AGG + chr2.4:62098416-62098435 MS.gene02916:CDS 40.0%
TATAAGACTTACAGGGCAAC+CGG - chr2.4:62097356-62097375 None:intergenic 40.0%
TATACGACCACCATCAACTT+TGG - chr2.4:62098619-62098638 None:intergenic 40.0%
TCATCATATCTCGAACCTAG+TGG - chr2.4:62098036-62098055 None:intergenic 40.0%
TCCATCGGGTGTTTATAAGA+GGG + chr2.4:62097063-62097082 MS.gene02916:CDS 40.0%
TGAGATGCCTCTTGGAATAT+GGG + chr2.4:62097672-62097691 MS.gene02916:CDS 40.0%
TGTGTCAGAAGAGAATCCAT+CGG + chr2.4:62097048-62097067 MS.gene02916:CDS 40.0%
TTAGAGAAAAGAACAGGCAC+AGG + chr2.4:62097391-62097410 MS.gene02916:CDS 40.0%
TTCTCACAACCACGAGTTAT+TGG + chr2.4:62097249-62097268 MS.gene02916:CDS 40.0%
TTGATAGGATCATGAAGGGT+TGG + chr2.4:62099290-62099309 MS.gene02916:intron 40.0%
TTGCACAACATAAGAGCCAT+TGG - chr2.4:62098589-62098608 None:intergenic 40.0%
TTGGGCCTTAGCTTATTTGA+AGG + chr2.4:62098191-62098210 MS.gene02916:intron 40.0%
TTTGCTGGAATGACAACAAC+AGG + chr2.4:62098222-62098241 MS.gene02916:CDS 40.0%
! AGGGAATTTGTTTGTCATCG+TGG + chr2.4:62097082-62097101 MS.gene02916:CDS 40.0%
! AGTCACTAACATTGATGGGT+GGG + chr2.4:62096799-62096818 MS.gene02916:CDS 40.0%
! ATGAGGGGATTCCTTTTGAA+GGG + chr2.4:62096921-62096940 MS.gene02916:CDS 40.0%
! ATGGTCTCACAGTTTGGTTT+GGG + chr2.4:62098126-62098145 MS.gene02916:CDS 40.0%
! GAATTTGTTTGTCATCGTGG+TGG + chr2.4:62097085-62097104 MS.gene02916:CDS 40.0%
! TGATTAGTTCCACCACAATC+TGG - chr2.4:62099125-62099144 None:intergenic 40.0%
!! CATCATACCAAAGTTGATGG+TGG + chr2.4:62098609-62098628 MS.gene02916:CDS 40.0%
!! TGGGTTGGTGTTGATAATCA+TGG + chr2.4:62097691-62097710 MS.gene02916:CDS 40.0%
!! TTCCAAGAGGCATCTCATAA+CGG - chr2.4:62097669-62097688 None:intergenic 40.0%
!!! CCTTTTGGTCTTGATCCTTT+TGG - chr2.4:62099062-62099081 None:intergenic 40.0%
AACCGTTATGAGATGCCTCT+TGG + chr2.4:62097664-62097683 MS.gene02916:CDS 45.0%
AACGTCACAGTCAGCATTGT+TGG - chr2.4:62099006-62099025 None:intergenic 45.0%
ACACCAACCCATATTCCAAG+AGG - chr2.4:62097682-62097701 None:intergenic 45.0%
ACCACGCAAAACTGTTGAAG+TGG + chr2.4:62097117-62097136 MS.gene02916:CDS 45.0%
AGGATCAAGACCAAAAGGAG+GGG + chr2.4:62099064-62099083 MS.gene02916:CDS 45.0%
ATGCCTCTTGGAATATGGGT+TGG + chr2.4:62097676-62097695 MS.gene02916:CDS 45.0%
CGTTGTCATGTTCCAGATTG+TGG + chr2.4:62099110-62099129 MS.gene02916:CDS 45.0%
CTTTCTCGTCTCCATCGAAA+AGG - chr2.4:62098774-62098793 None:intergenic 45.0%
GACGAGGCATGTTCTTCAAT+AGG - chr2.4:62098489-62098508 None:intergenic 45.0%
GTGTCAGAAGAGAATCCATC+GGG + chr2.4:62097049-62097068 MS.gene02916:CDS 45.0%
GTTTGTCATCGTGGTGGTAT+TGG + chr2.4:62097091-62097110 MS.gene02916:CDS 45.0%
TACCTTTATAGCCCATGCGA+AGG - chr2.4:62097767-62097786 None:intergenic 45.0%
TCCCTCTTATAAACACCCGA+TGG - chr2.4:62097067-62097086 None:intergenic 45.0%
TGTCATGTTCCAGATTGTGG+TGG + chr2.4:62099113-62099132 MS.gene02916:CDS 45.0%
TTGTCGCAAAGCTGTCAAGT+TGG + chr2.4:62098001-62098020 MS.gene02916:CDS 45.0%
! GATGAGGGGATTCCTTTTGA+AGG + chr2.4:62096920-62096939 MS.gene02916:CDS 45.0%
! TCCACTTCAACAGTTTTGCG+TGG - chr2.4:62097121-62097140 None:intergenic 45.0%
! TGGCATTGAGGAAAAATGGG+TGG + chr2.4:62097210-62097229 MS.gene02916:CDS 45.0%
! TTCCTTCGCATGGGCTATAA+AGG + chr2.4:62097762-62097781 MS.gene02916:CDS 45.0%
!! ATGGGTGGGTTCCTTTTGAA+GGG + chr2.4:62096813-62096832 MS.gene02916:CDS 45.0%
AACGAGAACAGGTTGCCACA+AGG + chr2.4:62098901-62098920 MS.gene02916:CDS 50.0%
ACGAGAACAGGTTGCCACAA+GGG + chr2.4:62098902-62098921 MS.gene02916:CDS 50.0%
ATGACAACGACGAGGCTTCT+TGG - chr2.4:62099099-62099118 None:intergenic 50.0%
GCAGCAATTGAAGCTGCCAA+TGG + chr2.4:62098570-62098589 MS.gene02916:CDS 50.0%
! TCTGGAACATGACAACGACG+AGG - chr2.4:62099107-62099126 None:intergenic 50.0%
!! CTCACAGTTTGGTTTGGGTG+TGG + chr2.4:62098131-62098150 MS.gene02916:intron 50.0%
!! GATGGGTGGGTTCCTTTTGA+AGG + chr2.4:62096812-62096831 MS.gene02916:CDS 50.0%
! GCAGCTTCAACCCCTCCTTT+TGG - chr2.4:62099077-62099096 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 62096752 62099318 62096752 ID=MS.gene02916
chr2.4 mRNA 62096752 62099318 62096752 ID=MS.gene02916.t1;Parent=MS.gene02916
chr2.4 exon 62096752 62097783 62096752 ID=MS.gene02916.t1.exon1;Parent=MS.gene02916.t1
chr2.4 CDS 62096752 62097783 62096752 ID=cds.MS.gene02916.t1;Parent=MS.gene02916.t1
chr2.4 exon 62097877 62098147 62097877 ID=MS.gene02916.t1.exon2;Parent=MS.gene02916.t1
chr2.4 CDS 62097877 62098147 62097877 ID=cds.MS.gene02916.t1;Parent=MS.gene02916.t1
chr2.4 exon 62098202 62098287 62098202 ID=MS.gene02916.t1.exon3;Parent=MS.gene02916.t1
chr2.4 CDS 62098202 62098287 62098202 ID=cds.MS.gene02916.t1;Parent=MS.gene02916.t1
chr2.4 exon 62098396 62098638 62098396 ID=MS.gene02916.t1.exon4;Parent=MS.gene02916.t1
chr2.4 CDS 62098396 62098638 62098396 ID=cds.MS.gene02916.t1;Parent=MS.gene02916.t1
chr2.4 exon 62098858 62099210 62098858 ID=MS.gene02916.t1.exon5;Parent=MS.gene02916.t1
chr2.4 CDS 62098858 62099210 62098858 ID=cds.MS.gene02916.t1;Parent=MS.gene02916.t1
chr2.4 exon 62099297 62099318 62099297 ID=MS.gene02916.t1.exon6;Parent=MS.gene02916.t1
chr2.4 CDS 62099297 62099318 62099297 ID=cds.MS.gene02916.t1;Parent=MS.gene02916.t1
Gene Sequence

>MS.gene02916

ATGATATCTGAAGTAAGCAATGATGCTAGTGATTGGCCCTTGCCTCAAGTCACTAACATTGATGGGTGGGTTCCTTTTGAAGGGCAAATATTTGAAGATGATGAATATGTAAATTCTTTAACATTTCAAGAGCTACAACATGAACCCTTGCCTCAAGTCAATAACATTGATGAGGGGATTCCTTTTGAAGGGAAAATATTTGAAAGTGATAATGCTGCTTTTGAATTTTATTGTTTATTTGCAAAACAAAATGGATTTTCTATTAGGCGTGATCACATTTATAAATCTTGTAAATTTGTGTCAGAAGAGAATCCATCGGGTGTTTATAAGAGGGAATTTGTTTGTCATCGTGGTGGTATTGGTAAACCACGCAAAACTGTTGAAGTGGAAAATCAAAGGAAACGAAAATCTTCAAGATGTGATTGTAGTGCAAAGATGGTAGTTACTAAGAGAAGAATTGGCATTGAGGAAAAATGGGTGGTTAAACATCTCAACAATTCTCACAACCACGAGTTATTGGATGACAAAGAGGTTCAATTTCTTCCTGCTTATCGTAACATTCCAATTGTTGATCAAGATCGCATACTATTATTGTCTAAAGCCGGTTGCCCTGTAAGTCTTATAGCGAGAGTGCTTGAGTTAGAGAAAAGAACAGGCACAGGAAATTTACCATTCTTAGAAAAAGATATTAGAAATTTTATTCAATCATATAGTGGTATTGGTAAAGAAAGTGATGCTTCAGATGTTCTCAGATTGTGCAAAAGTTTGAAAGATCGAGATGAGGCCTTCCAATATGAATTCACAATTGATGGAAGTAACAAATTGGAGCATATTGTTTGGGCATTTGGTGATTCAATTCGTGCATATGAAGCTTTTGGAGATGTTGTAGTTTTTGATACCACATATAGAATAAACCGTTATGAGATGCCTCTTGGAATATGGGTTGGTGTTGATAATCATGGAAATTCAATTTTTTTTGGTTGCGTTCTATTGCGAAATGAGAAGATTTCTTCCTTCGCATGGGCTATAAAGGTAAATTTCTATTTACTCAAAGTTGCTATCACTGTTAAATTTTTTAATTTGAAACTTTATTAAATTATTTTATATTTATGTTACTATTTTCAGAGTTTCCTAGCTTTTGTGAAAGGAAAATATCCAAAAACAATTCTTACCGATCAAGATCCTTCAATTAAAGAAGCCATTGCAATGGAATTGCCAAACACAAAACATGCATTTTGCATATGGCATATTGTCGCAAAGCTGTCAAGTTGGTTTTCTTTTCCACTAGGTTCGAGATATGATGACTTTAAACATGAGTTTCATAAGGTGTATCATTTAGAATGTGCAGATGATTTTGAACGAGAATGGAAAGTAATGGTCTCACAGTTTGGTTTGGGTGTGGATAAGCATATTGACTTGCTCTTTACCCATCGTAAATTTTGGGCCTTAGCTTATTTGAAGGACTTTTTTTTTGCTGGAATGACAACAACAGGACGCTCAGAATCGATAAATTCATATATAAAAAGATTCTTAGATGCCAAAACAAGTTTGACAGATTTTGTAAATCAAGTAAGAACTTACTTGATACATTTTTTTTAATCAATTAGCTTATAATTTAGTCATGATTGTTATATTTTTGTGTAGGTTGGTGTTGCTGTCAAAATTAGAAATCAAGCTGGAGAAGAGGCACGAATGCGTCAAAAGTATCATAATCCTCATATTACAACACATTTTCCTATTGAAGAACATGCCTCGTCTATACTCACACCTTATGCCTTTAAGTTGCTTCAACATGAGATCGAATTATCTGCAAAGTATGCAGCAATTGAAGCTGCCAATGGCTCTTATGTTGTGCAACATCATACCAAAGTTGATGGTGGTCGTATAGTAAGTTTGATAAAAGAAGAAGAGTCAATTCATTGTTCTTGTAAAGAGTTTCAATTTTCAGGAATAATATGTAGACATGCAATTCGAGTGCTAATGTTGAAAAATTATTTTGTTCTTCCATCAAAATATCTTCCTTTTCGATGGAGACGAGAAAGTTCATTGATCCCAAAATCTAGTCATGTAATAAACTGCAATGATGGATCCTCTGCTAAATTTTAGTCTCTAATTCGATGTTTGGAAGTCGAATCATTAAAGACAAGTGAACGAGAACAGGTTGCCACAAGGGAGTTAGAAAATATCATCATAAAGATAAAAGGCATGCCTGAAACTCAAGAACATGTGATTGATTTGGAAGAGGATGTTTCCAACAATGCTGACTGTGACGTTGAAAATCCTGTTGTTTCAAAAACAAAAGGTCGGCCAAAAGGATCAAGACCAAAAGGAGGGGTTGAAGCTGCCAAGAAGCCTCGTCGTTGTCATGTTCCAGATTGTGGTGGAACTAATCACGACTCACGAAACTGTCCAAACAAAAAGAGAAAAATGGAAGTGTTGCCTTCTCAATCTCCTAATAAGTAAGTTTTAATTGGTGTGTCTTGCTCTACTCATGAGTAATATATTGAATTGTAATATTAACTGTATTTTTATGGTGCTTTGATAGGATCATGAAGGGTTGGAAGTAG
Protein sequence

>MS.gene02916.t1

MISEVSNDASDWPLPQVTNIDGWVPFEGQIFEDDEYVNSLTFQELQHEPLPQVNNIDEGIPFEGKIFESDNAAFEFYCLFAKQNGFSIRRDHIYKSCKFVSEENPSGVYKREFVCHRGGIGKPRKTVEVENQRKRKSSRCDCSAKMVVTKRRIGIEEKWVVKHLNNSHNHELLDDKEVQFLPAYRNIPIVDQDRILLLSKAGCPVSLIARVLELEKRTGTGNLPFLEKDIRNFIQSYSGIGKESDASDVLRLCKSLKDRDEAFQYEFTIDGSNKLEHIVWAFGDSIRAYEAFGDVVVFDTTYRINRYEMPLGIWVGVDNHGNSIFFGCVLLRNEKISSFAWAIKSFLAFVKGKYPKTILTDQDPSIKEAIAMELPNTKHAFCIWHIVAKLSSWFSFPLGSRYDDFKHEFHKVYHLECADDFEREWKVMVSQFGLAYLKDFFFAGMTTTGRSESINSYIKRFLDVGVAVKIRNQAGEEARMRQKYHNPHITTHFPIEEHASSILTPYAFKLLQHEIELSAKYAAIEAANGSYVVQHHTKVDGGRISLIRCLEVESLKTSEREQVATRELENIIIKIKGMPETQEHVIDLEEDVSNNADCDVENPVVSKTKGRPKGSRPKGGVEAAKKPRRCHVPDCGGTNHDSRNCPNKKRKMEVLPSQSPNKIMKGWK