Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029282.t1 | XP_003601199.1 | 53.2 | 363 | 40 | 12 | 1 | 247 | 1 | 349 | 2.00E-78 | 302.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029282.t1 | Q8LPH6 | 49.6 | 133 | 14 | 1 | 1 | 80 | 1 | 133 | 1.8e-27 | 124.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029282.t1 | G7J4T2 | 53.2 | 363 | 40 | 12 | 1 | 247 | 1 | 349 | 1.4e-78 | 302.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene029282.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029282.t1 | MTR_3g077110 | 52.617 | 363 | 42 | 11 | 1 | 247 | 1 | 349 | 6.35e-104 | 305 |
| MS.gene029282.t1 | MTR_1g112760 | 54.762 | 252 | 25 | 7 | 3 | 190 | 4 | 230 | 5.73e-71 | 217 |
| MS.gene029282.t1 | MTR_4g105130 | 36.314 | 369 | 105 | 13 | 1 | 247 | 1 | 361 | 1.64e-51 | 171 |
| MS.gene029282.t1 | MTR_7g110830 | 45.139 | 144 | 26 | 1 | 1 | 91 | 1 | 144 | 2.60e-30 | 116 |
| MS.gene029282.t1 | MTR_1g085040 | 48.062 | 129 | 14 | 1 | 1 | 76 | 1 | 129 | 1.28e-29 | 114 |
| MS.gene029282.t1 | MTR_1g085640 | 42.384 | 151 | 30 | 2 | 1 | 94 | 1 | 151 | 1.74e-28 | 111 |
| MS.gene029282.t1 | MTR_7g111290 | 47.727 | 132 | 16 | 1 | 1 | 79 | 1 | 132 | 3.15e-24 | 99.8 |
| MS.gene029282.t1 | MTR_2g099740 | 40.000 | 125 | 22 | 1 | 1 | 72 | 1 | 125 | 2.33e-22 | 95.1 |
| MS.gene029282.t1 | MTR_8g017500 | 36.879 | 141 | 30 | 2 | 1 | 82 | 1 | 141 | 4.56e-22 | 93.6 |
| MS.gene029282.t1 | MTR_8g017440 | 39.516 | 124 | 22 | 1 | 1 | 71 | 1 | 124 | 6.10e-22 | 93.2 |
| MS.gene029282.t1 | MTR_8g017540 | 38.710 | 124 | 23 | 1 | 1 | 71 | 47 | 170 | 3.17e-21 | 91.7 |
| MS.gene029282.t1 | MTR_2g064160 | 38.889 | 126 | 24 | 1 | 4 | 76 | 3 | 128 | 1.13e-20 | 89.4 |
| MS.gene029282.t1 | MTR_6g009430 | 34.884 | 129 | 31 | 1 | 1 | 76 | 1 | 129 | 1.18e-20 | 89.4 |
| MS.gene029282.t1 | MTR_5g078140 | 37.984 | 129 | 27 | 1 | 1 | 76 | 1 | 129 | 8.02e-20 | 87.0 |
| MS.gene029282.t1 | MTR_4g073420 | 34.884 | 129 | 31 | 1 | 1 | 76 | 1 | 129 | 1.75e-19 | 85.5 |
| MS.gene029282.t1 | MTR_8g095390 | 37.209 | 129 | 28 | 1 | 1 | 76 | 1 | 129 | 2.45e-19 | 85.5 |
| MS.gene029282.t1 | MTR_8g017350 | 38.400 | 125 | 23 | 2 | 1 | 71 | 1 | 125 | 3.77e-19 | 86.7 |
| MS.gene029282.t1 | MTR_3g065440 | 38.168 | 131 | 28 | 1 | 1 | 78 | 1 | 131 | 4.68e-19 | 84.7 |
| MS.gene029282.t1 | MTR_8g017340 | 38.400 | 125 | 23 | 2 | 1 | 71 | 1 | 125 | 5.64e-19 | 85.9 |
| MS.gene029282.t1 | MTR_3g097450 | 29.630 | 162 | 53 | 2 | 1 | 109 | 1 | 154 | 7.89e-19 | 84.0 |
| MS.gene029282.t1 | MTR_4g478180 | 33.824 | 136 | 37 | 1 | 1 | 83 | 2 | 137 | 8.91e-19 | 84.3 |
| MS.gene029282.t1 | MTR_4g082230 | 35.938 | 128 | 29 | 1 | 1 | 75 | 1 | 128 | 1.02e-18 | 84.7 |
| MS.gene029282.t1 | MTR_8g017480 | 71.111 | 45 | 13 | 0 | 1 | 45 | 1 | 45 | 1.04e-18 | 77.4 |
| MS.gene029282.t1 | MTR_7g011170 | 33.908 | 174 | 54 | 3 | 1 | 121 | 1 | 166 | 1.06e-18 | 83.6 |
| MS.gene029282.t1 | MTR_4g102380 | 36.434 | 129 | 29 | 1 | 1 | 76 | 1 | 129 | 1.56e-18 | 82.8 |
| MS.gene029282.t1 | MTR_6g012690 | 36.719 | 128 | 28 | 1 | 1 | 75 | 1 | 128 | 2.13e-18 | 83.6 |
| MS.gene029282.t1 | MTR_8g098860 | 37.815 | 119 | 21 | 1 | 1 | 66 | 1 | 119 | 4.99e-18 | 82.0 |
| MS.gene029282.t1 | MTR_5g079670 | 33.094 | 139 | 35 | 2 | 3 | 83 | 2 | 140 | 5.32e-18 | 80.1 |
| MS.gene029282.t1 | MTR_4g082290 | 35.938 | 128 | 29 | 1 | 1 | 75 | 1 | 128 | 1.04e-17 | 82.0 |
| MS.gene029282.t1 | MTR_3g074520 | 34.375 | 128 | 31 | 1 | 1 | 75 | 1 | 128 | 1.72e-17 | 80.9 |
| MS.gene029282.t1 | MTR_8g017390 | 38.983 | 118 | 18 | 2 | 1 | 64 | 1 | 118 | 2.29e-17 | 81.3 |
| MS.gene029282.t1 | MTR_5g010020 | 42.353 | 85 | 47 | 1 | 1 | 83 | 1 | 85 | 1.16e-16 | 78.6 |
| MS.gene029282.t1 | MTR_2g089620 | 32.824 | 131 | 35 | 1 | 1 | 78 | 1 | 131 | 3.52e-16 | 77.0 |
| MS.gene029282.t1 | MTR_3g461490 | 28.079 | 203 | 84 | 4 | 1 | 150 | 1 | 194 | 4.65e-16 | 76.6 |
| MS.gene029282.t1 | MTR_5g029840 | 33.594 | 128 | 32 | 1 | 1 | 75 | 1 | 128 | 7.16e-16 | 76.3 |
| MS.gene029282.t1 | MTR_2g011660 | 33.333 | 129 | 32 | 2 | 1 | 75 | 1 | 129 | 8.79e-16 | 75.9 |
| MS.gene029282.t1 | MTR_5g007370 | 35.897 | 117 | 22 | 1 | 12 | 75 | 12 | 128 | 3.11e-15 | 73.9 |
| MS.gene029282.t1 | MTR_3g083540 | 35.484 | 124 | 27 | 1 | 6 | 76 | 35 | 158 | 3.86e-15 | 73.6 |
| MS.gene029282.t1 | MTR_4g091490 | 32.558 | 129 | 33 | 2 | 1 | 75 | 1 | 129 | 4.66e-15 | 73.9 |
| MS.gene029282.t1 | MTR_7g076740 | 34.615 | 130 | 31 | 2 | 1 | 76 | 1 | 130 | 1.42e-14 | 72.0 |
| MS.gene029282.t1 | MTR_4g485530 | 30.952 | 126 | 34 | 1 | 1 | 73 | 1 | 126 | 2.10e-13 | 67.0 |
| MS.gene029282.t1 | MTR_8g031360 | 34.351 | 131 | 31 | 3 | 1 | 76 | 1 | 131 | 9.34e-13 | 67.0 |
| MS.gene029282.t1 | MTR_1g017140 | 25.556 | 270 | 109 | 9 | 1 | 181 | 1 | 267 | 1.73e-12 | 66.6 |
| MS.gene029282.t1 | MTR_6g055910 | 29.615 | 260 | 98 | 9 | 9 | 183 | 11 | 270 | 2.04e-12 | 65.9 |
| MS.gene029282.t1 | MTR_4g065017 | 35.849 | 106 | 15 | 1 | 11 | 63 | 16 | 121 | 9.73e-12 | 64.3 |
| MS.gene029282.t1 | MTR_3g028740 | 35.185 | 108 | 17 | 1 | 9 | 63 | 19 | 126 | 1.09e-11 | 63.9 |
| MS.gene029282.t1 | MTR_5g042030 | 32.479 | 117 | 26 | 1 | 12 | 75 | 7 | 123 | 1.30e-11 | 63.5 |
| MS.gene029282.t1 | MTR_2g097910 | 35.238 | 105 | 15 | 1 | 12 | 63 | 20 | 124 | 2.30e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029282.t1 | AT5G26660 | 49.624 | 133 | 14 | 1 | 1 | 80 | 1 | 133 | 7.43e-34 | 125 |
| MS.gene029282.t1 | AT1G57560 | 48.872 | 133 | 15 | 1 | 1 | 80 | 1 | 133 | 1.49e-31 | 117 |
| MS.gene029282.t1 | AT1G57560 | 49.612 | 129 | 12 | 1 | 1 | 76 | 1 | 129 | 1.62e-31 | 118 |
| MS.gene029282.t1 | AT1G09540 | 45.139 | 144 | 24 | 2 | 1 | 91 | 1 | 142 | 2.05e-29 | 114 |
| MS.gene029282.t1 | AT4G01680 | 44.755 | 143 | 25 | 2 | 1 | 90 | 1 | 142 | 7.16e-29 | 109 |
| MS.gene029282.t1 | AT4G01680 | 44.755 | 143 | 25 | 2 | 1 | 90 | 1 | 142 | 3.20e-28 | 110 |
| MS.gene029282.t1 | AT5G61420 | 37.984 | 129 | 27 | 1 | 1 | 76 | 1 | 129 | 3.95e-21 | 91.3 |
| MS.gene029282.t1 | AT3G12720 | 39.024 | 123 | 22 | 1 | 4 | 73 | 14 | 136 | 5.11e-21 | 90.1 |
| MS.gene029282.t1 | AT1G66230 | 37.063 | 143 | 33 | 2 | 1 | 90 | 1 | 139 | 8.29e-21 | 89.0 |
| MS.gene029282.t1 | AT5G15310 | 32.308 | 195 | 66 | 4 | 1 | 137 | 1 | 187 | 1.33e-20 | 89.4 |
| MS.gene029282.t1 | AT3G13890 | 41.026 | 117 | 16 | 1 | 1 | 64 | 1 | 117 | 1.34e-20 | 89.7 |
| MS.gene029282.t1 | AT5G15310 | 32.308 | 195 | 66 | 4 | 1 | 137 | 1 | 187 | 1.42e-20 | 87.8 |
| MS.gene029282.t1 | AT5G15310 | 32.308 | 195 | 66 | 4 | 1 | 137 | 1 | 187 | 1.66e-20 | 89.0 |
| MS.gene029282.t1 | AT5G16600 | 34.932 | 146 | 42 | 1 | 1 | 93 | 1 | 146 | 7.16e-20 | 87.4 |
| MS.gene029282.t1 | AT4G22680 | 35.417 | 144 | 40 | 1 | 1 | 91 | 1 | 144 | 1.70e-19 | 85.1 |
| MS.gene029282.t1 | AT5G07690 | 36.434 | 129 | 29 | 1 | 1 | 76 | 1 | 129 | 3.01e-19 | 85.5 |
| MS.gene029282.t1 | AT4G34990 | 32.886 | 149 | 45 | 2 | 1 | 96 | 1 | 147 | 3.53e-19 | 84.7 |
| MS.gene029282.t1 | AT1G34670 | 35.938 | 128 | 29 | 1 | 1 | 75 | 1 | 128 | 3.92e-19 | 85.5 |
| MS.gene029282.t1 | AT4G01680 | 80.000 | 45 | 9 | 0 | 1 | 45 | 1 | 45 | 4.51e-19 | 85.5 |
| MS.gene029282.t1 | AT4G01680 | 45.238 | 84 | 43 | 3 | 8 | 90 | 73 | 154 | 5.40e-12 | 65.1 |
| MS.gene029282.t1 | AT2G16720 | 31.973 | 147 | 45 | 2 | 1 | 94 | 1 | 145 | 5.23e-19 | 84.0 |
| MS.gene029282.t1 | AT1G63910 | 35.556 | 135 | 34 | 1 | 1 | 82 | 1 | 135 | 6.60e-19 | 85.1 |
| MS.gene029282.t1 | AT3G01140 | 32.787 | 183 | 59 | 4 | 1 | 126 | 1 | 176 | 1.29e-18 | 84.0 |
| MS.gene029282.t1 | AT3G02940 | 34.375 | 128 | 31 | 1 | 1 | 75 | 1 | 128 | 1.33e-18 | 83.6 |
| MS.gene029282.t1 | AT3G02940 | 34.375 | 128 | 31 | 1 | 1 | 75 | 1 | 128 | 1.89e-18 | 83.2 |
| MS.gene029282.t1 | AT3G02940 | 34.375 | 128 | 31 | 1 | 1 | 75 | 1 | 128 | 1.89e-18 | 83.2 |
| MS.gene029282.t1 | AT5G60890 | 33.333 | 129 | 33 | 1 | 1 | 76 | 1 | 129 | 2.80e-18 | 82.4 |
| MS.gene029282.t1 | AT4G05100 | 34.884 | 129 | 30 | 2 | 1 | 75 | 1 | 129 | 7.48e-18 | 81.6 |
| MS.gene029282.t1 | AT4G12350 | 35.659 | 129 | 30 | 1 | 1 | 76 | 1 | 129 | 1.01e-17 | 80.9 |
| MS.gene029282.t1 | AT4G21440 | 29.310 | 174 | 56 | 2 | 1 | 121 | 1 | 160 | 1.11e-17 | 81.3 |
| MS.gene029282.t1 | AT4G38620 | 30.405 | 148 | 47 | 2 | 1 | 95 | 1 | 145 | 1.57e-17 | 80.1 |
| MS.gene029282.t1 | AT4G09460 | 31.544 | 149 | 42 | 2 | 1 | 96 | 1 | 142 | 2.60e-17 | 79.0 |
| MS.gene029282.t1 | AT5G07700 | 34.109 | 129 | 32 | 1 | 1 | 76 | 1 | 129 | 2.65e-17 | 80.1 |
| MS.gene029282.t1 | AT5G07700 | 34.109 | 129 | 32 | 1 | 1 | 76 | 1 | 129 | 2.65e-17 | 80.1 |
| MS.gene029282.t1 | AT3G01140 | 32.584 | 178 | 56 | 4 | 6 | 126 | 49 | 219 | 3.37e-17 | 80.5 |
| MS.gene029282.t1 | AT5G16770 | 33.594 | 128 | 32 | 1 | 1 | 75 | 1 | 128 | 5.26e-17 | 79.3 |
| MS.gene029282.t1 | AT5G16770 | 33.594 | 128 | 32 | 1 | 1 | 75 | 1 | 128 | 5.26e-17 | 79.3 |
| MS.gene029282.t1 | AT4G28110 | 32.031 | 128 | 34 | 1 | 1 | 75 | 1 | 128 | 5.57e-17 | 78.6 |
| MS.gene029282.t1 | AT1G22640 | 33.088 | 136 | 36 | 2 | 1 | 83 | 1 | 134 | 5.79e-17 | 78.2 |
| MS.gene029282.t1 | AT5G16770 | 33.594 | 128 | 32 | 1 | 1 | 75 | 1 | 128 | 7.40e-17 | 79.0 |
| MS.gene029282.t1 | AT5G16770 | 33.594 | 128 | 32 | 1 | 1 | 75 | 1 | 128 | 7.40e-17 | 79.0 |
| MS.gene029282.t1 | AT5G14340 | 34.109 | 129 | 32 | 1 | 1 | 76 | 13 | 141 | 7.70e-17 | 78.2 |
| MS.gene029282.t1 | AT3G13540 | 32.237 | 152 | 50 | 1 | 6 | 104 | 17 | 168 | 9.45e-17 | 77.4 |
| MS.gene029282.t1 | AT3G13890 | 37.736 | 106 | 55 | 1 | 1 | 95 | 1 | 106 | 1.14e-16 | 78.6 |
| MS.gene029282.t1 | AT4G17785 | 35.036 | 137 | 35 | 2 | 1 | 83 | 1 | 137 | 1.65e-16 | 78.2 |
| MS.gene029282.t1 | AT4G17785 | 35.036 | 137 | 35 | 2 | 1 | 83 | 1 | 137 | 1.70e-16 | 78.2 |
| MS.gene029282.t1 | AT1G35515 | 33.077 | 130 | 34 | 1 | 1 | 77 | 1 | 130 | 2.01e-16 | 75.9 |
| MS.gene029282.t1 | AT5G26660 | 51.351 | 74 | 34 | 2 | 8 | 80 | 30 | 102 | 2.14e-16 | 77.4 |
| MS.gene029282.t1 | AT3G61250 | 31.319 | 182 | 62 | 5 | 1 | 129 | 1 | 172 | 4.48e-16 | 76.3 |
| MS.gene029282.t1 | AT1G18570 | 33.077 | 130 | 33 | 2 | 1 | 76 | 1 | 130 | 4.74e-16 | 76.6 |
| MS.gene029282.t1 | AT5G56110 | 33.333 | 129 | 33 | 1 | 1 | 76 | 1 | 129 | 6.51e-16 | 76.3 |
| MS.gene029282.t1 | AT1G74080 | 30.070 | 143 | 47 | 1 | 1 | 90 | 1 | 143 | 1.18e-15 | 75.5 |
| MS.gene029282.t1 | AT5G54230 | 33.588 | 131 | 34 | 1 | 1 | 78 | 1 | 131 | 3.52e-15 | 73.9 |
| MS.gene029282.t1 | AT5G65230 | 33.333 | 132 | 35 | 1 | 1 | 79 | 1 | 132 | 6.42e-15 | 73.2 |
| MS.gene029282.t1 | AT5G10280 | 44.595 | 74 | 39 | 2 | 3 | 75 | 56 | 128 | 6.72e-15 | 73.6 |
| MS.gene029282.t1 | AT3G28470 | 35.659 | 129 | 30 | 1 | 1 | 76 | 1 | 129 | 1.03e-14 | 72.8 |
| MS.gene029282.t1 | AT1G74430 | 34.375 | 128 | 31 | 1 | 1 | 75 | 1 | 128 | 2.30e-14 | 71.2 |
| MS.gene029282.t1 | AT1G18710 | 42.857 | 70 | 40 | 0 | 8 | 77 | 61 | 130 | 1.65e-13 | 68.9 |
| MS.gene029282.t1 | AT1G22640 | 44.156 | 77 | 39 | 3 | 8 | 83 | 4 | 77 | 2.99e-13 | 67.0 |
| MS.gene029282.t1 | AT5G62320 | 29.412 | 153 | 44 | 3 | 2 | 90 | 3 | 155 | 8.23e-13 | 66.6 |
| MS.gene029282.t1 | AT5G61420 | 70.732 | 41 | 11 | 1 | 37 | 76 | 10 | 50 | 1.97e-12 | 65.9 |
| MS.gene029282.t1 | AT5G15310 | 39.252 | 107 | 51 | 4 | 37 | 137 | 10 | 108 | 2.51e-12 | 65.1 |
| MS.gene029282.t1 | AT3G48920 | 34.127 | 126 | 28 | 3 | 9 | 79 | 15 | 140 | 1.61e-11 | 63.2 |
| MS.gene029282.t1 | AT3G08500 | 24.111 | 253 | 113 | 7 | 4 | 198 | 19 | 250 | 2.57e-11 | 63.2 |
| MS.gene029282.t1 | AT4G28110 | 57.500 | 40 | 16 | 1 | 37 | 75 | 5 | 44 | 4.77e-11 | 60.8 |
| MS.gene029282.t1 | AT5G14340 | 52.000 | 50 | 23 | 1 | 28 | 76 | 2 | 51 | 6.06e-11 | 60.1 |
Find 39 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATACAGTTCATAATAAT+TGG | 0.135565 | 1.4:-86652440 | None:intergenic |
| TCTTCTGACAGCGATGTTAA+AGG | 0.277255 | 1.4:-86652378 | None:intergenic |
| AAGAGCTTCTTATCTTCTTC+GGG | 0.293638 | 1.4:-86651082 | None:intergenic |
| TTCAATTACATAACCATGTT+TGG | 0.311883 | 1.4:+86651103 | MS.gene029282:CDS |
| TTAAGATTACCAGCTAGTTT+AGG | 0.344561 | 1.4:-86651148 | None:intergenic |
| AAATACAGTTCATAATAATT+GGG | 0.349576 | 1.4:-86652439 | None:intergenic |
| TGAGGTGGCATTATCATTGA+TGG | 0.354750 | 1.4:-86651973 | None:intergenic |
| GAAGAGCTTCTTATCTTCTT+CGG | 0.373625 | 1.4:-86651083 | None:intergenic |
| AAGAGATTCATTATTGATCA+AGG | 0.401722 | 1.4:-86651929 | None:intergenic |
| CATCAACAAGACAGATTCAA+TGG | 0.439637 | 1.4:+86652341 | MS.gene029282:CDS |
| ATCAAGGGTTTATGTGTAGC+TGG | 0.461421 | 1.4:-86651913 | None:intergenic |
| TTCACTTCAAACTGCAACAA+AGG | 0.467959 | 1.4:-86652294 | None:intergenic |
| TACATAACCATGTTTGGTGT+TGG | 0.474556 | 1.4:+86651109 | MS.gene029282:CDS |
| AAGGCATTGAAGAGAAGAAC+TGG | 0.481895 | 1.4:-86652173 | None:intergenic |
| AGAGATTCATTATTGATCAA+GGG | 0.507195 | 1.4:-86651928 | None:intergenic |
| CCATGTTTGGTGTTGGATGT+TGG | 0.512330 | 1.4:+86651116 | MS.gene029282:CDS |
| TGAAAATTACAATAAGCTGT+TGG | 0.514835 | 1.4:+86652269 | MS.gene029282:CDS |
| AGTTCAGTTCCTAAACTAGC+TGG | 0.517760 | 1.4:+86651139 | MS.gene029282:CDS |
| TGTTCAAATTGTAATTGACT+TGG | 0.522549 | 1.4:-86652147 | None:intergenic |
| GATTAATGAAAATGCCTCAT+GGG | 0.535807 | 1.4:+86652242 | MS.gene029282:CDS |
| CAATAATGAATCTCTTGTAA+AGG | 0.536511 | 1.4:+86651936 | MS.gene029282:CDS |
| ATCTCTTGTAAAGGAAGAGA+AGG | 0.539524 | 1.4:+86651945 | MS.gene029282:CDS |
| ATGCTTAAATTGTAGTGCAA+TGG | 0.540486 | 1.4:-86652102 | None:intergenic |
| CCAACATCCAACACCAAACA+TGG | 0.541756 | 1.4:-86651116 | None:intergenic |
| AATGGATCAAAATCAAATGT+TGG | 0.545963 | 1.4:-86652084 | None:intergenic |
| AATTGTAATTGACTTGGCTG+AGG | 0.553273 | 1.4:-86652141 | None:intergenic |
| TGATTAATGAAAATGCCTCA+TGG | 0.558714 | 1.4:+86652241 | MS.gene029282:CDS |
| TGACAGCGATGTTAAAGGAT+AGG | 0.564100 | 1.4:-86652373 | None:intergenic |
| AATGAAAATGCCTCATGGGA+AGG | 0.570813 | 1.4:+86652246 | MS.gene029282:CDS |
| TGTGTAGCTAAAGTCCTTTG+AGG | 0.574260 | 1.4:-86651991 | None:intergenic |
| CAATGATAATGCCACCTCAA+AGG | 0.580674 | 1.4:+86651977 | MS.gene029282:CDS |
| AATACAGTTCATAATAATTG+GGG | 0.584426 | 1.4:-86652438 | None:intergenic |
| TGCTTCTGAATATTCATGTG+AGG | 0.611193 | 1.4:-86652028 | None:intergenic |
| AGAGCTTCTTATCTTCTTCG+GGG | 0.620275 | 1.4:-86651081 | None:intergenic |
| AAGAAGAAACTAATGAAACA+AGG | 0.624080 | 1.4:+86651883 | MS.gene029282:CDS |
| GAGCTTCTTATCTTCTTCGG+GGG | 0.637567 | 1.4:-86651080 | None:intergenic |
| AGAAGAAACTAATGAAACAA+GGG | 0.641105 | 1.4:+86651884 | MS.gene029282:CDS |
| TCACTTTCAATATTTAGACA+AGG | 0.664139 | 1.4:-86652192 | None:intergenic |
| GTAGCTAAAGTCCTTTGAGG+TGG | 0.669433 | 1.4:-86651988 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATGAGATAAAAAACTAT+TGG | + | chr1.4:86651848-86651867 | MS.gene029282:CDS | 15.0% |
| !! | AAAAACTAAGAAAAGGTTTA+TGG | + | chr1.4:86651056-86651075 | MS.gene029282:CDS | 20.0% |
| !! | TCATAAAAATTATATACGCT+TGG | - | chr1.4:86651538-86651557 | None:intergenic | 20.0% |
| !! | TTAAAGCAAAAACTAAGAAA+AGG | + | chr1.4:86651049-86651068 | MS.gene029282:CDS | 20.0% |
| !! | TTGGTAAAGGAAAAAATATA+AGG | - | chr1.4:86651519-86651538 | None:intergenic | 20.0% |
| !!! | ATATAATTTTTATGAGTTGC+AGG | + | chr1.4:86651543-86651562 | MS.gene029282:intron | 20.0% |
| !!! | TATAATTTTTATGAGTTGCA+GGG | + | chr1.4:86651544-86651563 | MS.gene029282:intron | 20.0% |
| ! | AAATTATATACGCTTGGTAA+AGG | - | chr1.4:86651532-86651551 | None:intergenic | 25.0% |
| ! | AAGAAGAAACTAATGAAACA+AGG | + | chr1.4:86651883-86651902 | MS.gene029282:CDS | 25.0% |
| ! | AAGAGATTCATTATTGATCA+AGG | - | chr1.4:86651932-86651951 | None:intergenic | 25.0% |
| ! | AATGGATCAAAATCAAATGT+TGG | - | chr1.4:86652087-86652106 | None:intergenic | 25.0% |
| ! | AGAAGAAACTAATGAAACAA+GGG | + | chr1.4:86651884-86651903 | MS.gene029282:CDS | 25.0% |
| ! | AGAGATTCATTATTGATCAA+GGG | - | chr1.4:86651931-86651950 | None:intergenic | 25.0% |
| ! | CAATAATGAATCTCTTGTAA+AGG | + | chr1.4:86651936-86651955 | MS.gene029282:CDS | 25.0% |
| ! | TCACTTTCAATATTTAGACA+AGG | - | chr1.4:86652195-86652214 | None:intergenic | 25.0% |
| ! | TGAAAATTACAATAAGCTGT+TGG | + | chr1.4:86652269-86652288 | MS.gene029282:CDS | 25.0% |
| ! | TGAGATGGATAAACTATTTA+AGG | + | chr1.4:86651595-86651614 | MS.gene029282:intron | 25.0% |
| ! | TGTTCAAATTGTAATTGACT+TGG | - | chr1.4:86652150-86652169 | None:intergenic | 25.0% |
| ! | TTCAATTACATAACCATGTT+TGG | + | chr1.4:86651103-86651122 | MS.gene029282:CDS | 25.0% |
| !!! | TAGTTTTTGCTTTAAACAAC+AGG | - | chr1.4:86651044-86651063 | None:intergenic | 25.0% |
| AAATTGCATCACAATTACCA+GGG | + | chr1.4:86651818-86651837 | MS.gene029282:intron | 30.0% | |
| ATAGTTGCTGTATATCTTGT+TGG | - | chr1.4:86651224-86651243 | None:intergenic | 30.0% | |
| ATGCTTAAATTGTAGTGCAA+TGG | - | chr1.4:86652105-86652124 | None:intergenic | 30.0% | |
| GATTAATGAAAATGCCTCAT+GGG | + | chr1.4:86652242-86652261 | MS.gene029282:CDS | 30.0% | |
| TAGTTGCTGTATATCTTGTT+GGG | - | chr1.4:86651223-86651242 | None:intergenic | 30.0% | |
| TGATTAATGAAAATGCCTCA+TGG | + | chr1.4:86652241-86652260 | MS.gene029282:CDS | 30.0% | |
| ! | TGAGAATATGATGAAAGTGT+GGG | - | chr1.4:86651310-86651329 | None:intergenic | 30.0% |
| ! | TTAAGATTACCAGCTAGTTT+AGG | - | chr1.4:86651151-86651170 | None:intergenic | 30.0% |
| ! | TTGGGTTGTACAAGTATATA+TGG | - | chr1.4:86651205-86651224 | None:intergenic | 30.0% |
| AAGAGCTTCTTATCTTCTTC+GGG | - | chr1.4:86651085-86651104 | None:intergenic | 35.0% | |
| AGGAATGTTCTCAAAACAAG+AGG | + | chr1.4:86651632-86651651 | MS.gene029282:intron | 35.0% | |
| ATCTCTTGTAAAGGAAGAGA+AGG | + | chr1.4:86651945-86651964 | MS.gene029282:CDS | 35.0% | |
| ATGGCATTCATGCATGTTTA+TGG | - | chr1.4:86651186-86651205 | None:intergenic | 35.0% | |
| ATTTCAAATGATGCCTCAGA+TGG | + | chr1.4:86651791-86651810 | MS.gene029282:intron | 35.0% | |
| CAAATTGCATCACAATTACC+AGG | + | chr1.4:86651817-86651836 | MS.gene029282:intron | 35.0% | |
| CATCAACAAGACAGATTCAA+TGG | + | chr1.4:86652341-86652360 | MS.gene029282:CDS | 35.0% | |
| GAAGAGCTTCTTATCTTCTT+CGG | - | chr1.4:86651086-86651105 | None:intergenic | 35.0% | |
| GTGAGAATATGATGAAAGTG+TGG | - | chr1.4:86651311-86651330 | None:intergenic | 35.0% | |
| TGCTTCTGAATATTCATGTG+AGG | - | chr1.4:86652031-86652050 | None:intergenic | 35.0% | |
| TTCACTTCAAACTGCAACAA+AGG | - | chr1.4:86652297-86652316 | None:intergenic | 35.0% | |
| TTTCAAATGATGCCTCAGAT+GGG | + | chr1.4:86651792-86651811 | MS.gene029282:intron | 35.0% | |
| ! | TGGATAAACTATTTAAGGCC+TGG | + | chr1.4:86651600-86651619 | MS.gene029282:intron | 35.0% |
| ! | TTCATGAAGCACTTGGAAAT+AGG | + | chr1.4:86651673-86651692 | MS.gene029282:intron | 35.0% |
| !! | AATTGTAATTGACTTGGCTG+AGG | - | chr1.4:86652144-86652163 | None:intergenic | 35.0% |
| !! | TACATAACCATGTTTGGTGT+TGG | + | chr1.4:86651109-86651128 | MS.gene029282:CDS | 35.0% |
| AACATTCCTCTCTTCAAACC+AGG | - | chr1.4:86651621-86651640 | None:intergenic | 40.0% | |
| AATGAAAATGCCTCATGGGA+AGG | + | chr1.4:86652246-86652265 | MS.gene029282:CDS | 40.0% | |
| AGAGCTTCTTATCTTCTTCG+GGG | - | chr1.4:86651084-86651103 | None:intergenic | 40.0% | |
| AGTTCAGTTCCTAAACTAGC+TGG | + | chr1.4:86651139-86651158 | MS.gene029282:CDS | 40.0% | |
| ATCAAGGGTTTATGTGTAGC+TGG | - | chr1.4:86651916-86651935 | None:intergenic | 40.0% | |
| ATCTCATTATCTGTTCTCCC+TGG | - | chr1.4:86651838-86651857 | None:intergenic | 40.0% | |
| GAAAGAGCTGTAGATTGAGA+TGG | + | chr1.4:86651580-86651599 | MS.gene029282:intron | 40.0% | |
| GTGGGTATTTGAATTGTGAG+AGG | - | chr1.4:86651292-86651311 | None:intergenic | 40.0% | |
| TCTTCTGACAGCGATGTTAA+AGG | - | chr1.4:86652381-86652400 | None:intergenic | 40.0% | |
| TGACAGCGATGTTAAAGGAT+AGG | - | chr1.4:86652376-86652395 | None:intergenic | 40.0% | |
| TGAGGTGGCATTATCATTGA+TGG | - | chr1.4:86651976-86651995 | None:intergenic | 40.0% | |
| TGTGTAGCTAAAGTCCTTTG+AGG | - | chr1.4:86651994-86652013 | None:intergenic | 40.0% | |
| ! | CAATGATAATGCCACCTCAA+AGG | + | chr1.4:86651977-86651996 | MS.gene029282:CDS | 40.0% |
| ! | GTAATTTTCACCTTCCCATG+AGG | - | chr1.4:86652259-86652278 | None:intergenic | 40.0% |
| ! | GTCAATCTTCATGAAGCACT+TGG | + | chr1.4:86651666-86651685 | MS.gene029282:intron | 40.0% |
| !! | AAGGCATTGAAGAGAAGAAC+TGG | - | chr1.4:86652176-86652195 | None:intergenic | 40.0% |
| ATGCAATTTGAGCCCATCTG+AGG | - | chr1.4:86651807-86651826 | None:intergenic | 45.0% | |
| CCAACATCCAACACCAAACA+TGG | - | chr1.4:86651119-86651138 | None:intergenic | 45.0% | |
| GAGCTTCTTATCTTCTTCGG+GGG | - | chr1.4:86651083-86651102 | None:intergenic | 45.0% | |
| ! | GTAGCTAAAGTCCTTTGAGG+TGG | - | chr1.4:86651991-86652010 | None:intergenic | 45.0% |
| ! | TTAAGGCCTGGTTTGAAGAG+AGG | + | chr1.4:86651612-86651631 | MS.gene029282:intron | 45.0% |
| !! | CCATGTTTGGTGTTGGATGT+TGG | + | chr1.4:86651116-86651135 | MS.gene029282:CDS | 45.0% |
| TATGAGTTGCAGGGTTGCGA+AGG | + | chr1.4:86651553-86651572 | MS.gene029282:intron | 50.0% | |
| TTCTCCCGTGCGTTGCAACA+CGG | + | chr1.4:86651359-86651378 | MS.gene029282:intron | 55.0% | |
| TTGACCCGTGTTGCAACGCA+CGG | - | chr1.4:86651367-86651386 | None:intergenic | 55.0% | |
| GTTGCAGGGTTGCGAAGGTG+TGG | + | chr1.4:86651558-86651577 | MS.gene029282:intron | 60.0% | |
| TCTCCCGTGCGTTGCAACAC+GGG | + | chr1.4:86651360-86651379 | MS.gene029282:intron | 60.0% | |
| TGACCCGTGTTGCAACGCAC+GGG | - | chr1.4:86651366-86651385 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 86651028 | 86652452 | 86651028 | ID=MS.gene029282 |
| chr1.4 | mRNA | 86651028 | 86652452 | 86651028 | ID=MS.gene029282.t1;Parent=MS.gene029282 |
| chr1.4 | exon | 86651028 | 86651160 | 86651028 | ID=MS.gene029282.t1.exon1;Parent=MS.gene029282.t1 |
| chr1.4 | CDS | 86651028 | 86651160 | 86651028 | ID=cds.MS.gene029282.t1;Parent=MS.gene029282.t1 |
| chr1.4 | exon | 86651839 | 86652452 | 86651839 | ID=MS.gene029282.t1.exon2;Parent=MS.gene029282.t1 |
| chr1.4 | CDS | 86651839 | 86652452 | 86651839 | ID=cds.MS.gene029282.t1;Parent=MS.gene029282.t1 |
| Gene Sequence |
| Protein sequence |
