Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029349.t1 | XP_013470240.1 | 93.4 | 182 | 7 | 2 | 1 | 177 | 1 | 182 | 2.00E-88 | 335.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029349.t1 | Q9FMC8 | 49.5 | 206 | 62 | 9 | 1 | 177 | 1 | 193 | 2.2e-32 | 140.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029349.t1 | A0A072VS70 | 93.4 | 182 | 7 | 2 | 1 | 177 | 1 | 182 | 1.4e-88 | 335.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene029349.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029349.t1 | MTR_1g111640 | 93.407 | 182 | 7 | 2 | 1 | 177 | 1 | 182 | 1.01e-122 | 346 |
| MS.gene029349.t1 | MTR_7g105780 | 49.741 | 193 | 70 | 8 | 1 | 178 | 1 | 181 | 3.64e-48 | 158 |
| MS.gene029349.t1 | MTR_1g058000 | 39.683 | 189 | 84 | 6 | 6 | 178 | 1 | 175 | 5.76e-31 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029349.t1 | AT5G04820 | 51.456 | 206 | 58 | 9 | 1 | 177 | 1 | 193 | 1.09e-49 | 161 |
| MS.gene029349.t1 | AT2G36050 | 46.809 | 94 | 34 | 5 | 95 | 177 | 66 | 154 | 6.30e-17 | 76.3 |
| MS.gene029349.t1 | AT3G52540 | 50.562 | 89 | 38 | 4 | 95 | 177 | 93 | 181 | 4.80e-15 | 71.2 |
Find 26 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTATAATGTTCTCTGTT+TGG | 0.328276 | 1.4:+85622298 | None:intergenic |
| AACACAACACTTTCTTTGAA+TGG | 0.349626 | 1.4:+85622090 | None:intergenic |
| CTACAACGACGGCGAATCAT+TGG | 0.389604 | 1.4:-85622205 | MS.gene029349:CDS |
| GATCTCTTAAAATCTTCATA+TGG | 0.409145 | 1.4:+85622048 | None:intergenic |
| AGTCTCATGGTGTGAAAGAT+TGG | 0.409364 | 1.4:-85622008 | MS.gene029349:CDS |
| GATGTTGTGAACCATGATTT+TGG | 0.433746 | 1.4:+85622273 | None:intergenic |
| TGTGAAAGATTGGGAAGGTT+TGG | 0.450919 | 1.4:-85621998 | MS.gene029349:CDS |
| AGCTGGTATTTGAAAGTGAA+TGG | 0.468308 | 1.4:-85621964 | MS.gene029349:intron |
| TGGCAATTCTTACCCTCTTG+TGG | 0.498707 | 1.4:-85622402 | MS.gene029349:CDS |
| TAAGAGATCAATGGAAGAGA+TGG | 0.508969 | 1.4:-85622034 | MS.gene029349:CDS |
| AGAAAGTGTTGTGTTAGCTA+TGG | 0.521557 | 1.4:-85622082 | MS.gene029349:CDS |
| CACGAGTTCGATCTTAGAAA+AGG | 0.522622 | 1.4:-85622130 | MS.gene029349:CDS |
| TGATGATGACATATTCAAAA+CGG | 0.538193 | 1.4:-85622340 | MS.gene029349:CDS |
| TTATAGAAACACCAAAATCA+TGG | 0.542649 | 1.4:-85622284 | MS.gene029349:CDS |
| GTCTCATGGTGTGAAAGATT+GGG | 0.558996 | 1.4:-85622007 | MS.gene029349:CDS |
| TTAGAAAAGGCGAAAGCGAA+TGG | 0.571257 | 1.4:-85622117 | MS.gene029349:CDS |
| CACAAAGAAAACAACATCCA+TGG | 0.572198 | 1.4:-85622422 | MS.gene029349:CDS |
| CGGCGAATCATTGGAGACAT+TGG | 0.582049 | 1.4:-85622196 | MS.gene029349:CDS |
| CAAGAGGGTAAGAATTGCCA+TGG | 0.585631 | 1.4:+85622405 | None:intergenic |
| TGAGAAACTTGCAGATTCAG+AGG | 0.589678 | 1.4:+85622251 | None:intergenic |
| TGTGTTAGCTATGGAGTCAG+AGG | 0.627955 | 1.4:-85622073 | MS.gene029349:CDS |
| GAAGAGATGGTTGAGTCTCA+TGG | 0.637125 | 1.4:-85622021 | MS.gene029349:CDS |
| CATGGTGTGAAAGATTGGGA+AGG | 0.649930 | 1.4:-85622003 | MS.gene029349:CDS |
| ATTGGTACGAGGAGTTAAGT+CGG | 0.655000 | 1.4:-85622178 | MS.gene029349:CDS |
| GATTACTATTGCTACAACGA+CGG | 0.655187 | 1.4:-85622216 | MS.gene029349:CDS |
| TCATTGGAGACATTGGTACG+AGG | 0.730029 | 1.4:-85622189 | MS.gene029349:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGTACAACAACAAAAAA+AGG | + | chr1.4:85622467-85622486 | None:intergenic | 20.0% |
| !! | TGAATGGTAAAAATAATCAT+GGG | - | chr1.4:85622220-85622239 | MS.gene029349:CDS | 20.0% |
| !!! | TTTTCTTTGTGAAATTTCTT+TGG | + | chr1.4:85621737-85621756 | None:intergenic | 20.0% |
| ! | AATAATCATGGGTTTATTGT+TGG | - | chr1.4:85622231-85622250 | MS.gene029349:CDS | 25.0% |
| ! | GATCTCTTAAAATCTTCATA+TGG | + | chr1.4:85622123-85622142 | None:intergenic | 25.0% |
| ! | GTGAATGGTAAAAATAATCA+TGG | - | chr1.4:85622219-85622238 | MS.gene029349:CDS | 25.0% |
| ! | TTATAGAAACACCAAAATCA+TGG | - | chr1.4:85621884-85621903 | MS.gene029349:intron | 25.0% |
| ! | TTTCTATAATGTTCTCTGTT+TGG | + | chr1.4:85621873-85621892 | None:intergenic | 25.0% |
| !! | TGATGATGACATATTCAAAA+CGG | - | chr1.4:85621828-85621847 | MS.gene029349:intron | 25.0% |
| !!! | TGAAGATTTTAAGAGATCAA+TGG | - | chr1.4:85622125-85622144 | MS.gene029349:CDS | 25.0% |
| AACACAACACTTTCTTTGAA+TGG | + | chr1.4:85622081-85622100 | None:intergenic | 30.0% | |
| ! | ACTCGAAAAGAAAGAGTTTT+TGG | + | chr1.4:85621796-85621815 | None:intergenic | 30.0% |
| !! | GAAAAGAAAGAGTTTTTGGA+TGG | + | chr1.4:85621792-85621811 | None:intergenic | 30.0% |
| !!! | AAAACTCTTTCTTTTCGAGT+TGG | - | chr1.4:85621796-85621815 | MS.gene029349:intron | 30.0% |
| !!! | TCTTTGGTTTTGAAAAGAGA+AGG | + | chr1.4:85621721-85621740 | None:intergenic | 30.0% |
| AGCTGGTATTTGAAAGTGAA+TGG | - | chr1.4:85622204-85622223 | MS.gene029349:CDS | 35.0% | |
| CACAAAGAAAACAACATCCA+TGG | - | chr1.4:85621746-85621765 | MS.gene029349:intron | 35.0% | |
| GATTACTATTGCTACAACGA+CGG | - | chr1.4:85621952-85621971 | MS.gene029349:intron | 35.0% | |
| TAAGAGATCAATGGAAGAGA+TGG | - | chr1.4:85622134-85622153 | MS.gene029349:CDS | 35.0% | |
| TAGATACAAAGGTGAAGAAG+AGG | + | chr1.4:85622355-85622374 | None:intergenic | 35.0% | |
| TCACCTTTGTATCTATGTGA+GGG | - | chr1.4:85622360-85622379 | MS.gene029349:CDS | 35.0% | |
| TTCACCTTTGTATCTATGTG+AGG | - | chr1.4:85622359-85622378 | MS.gene029349:CDS | 35.0% | |
| ! | GATGTTGTGAACCATGATTT+TGG | + | chr1.4:85621898-85621917 | None:intergenic | 35.0% |
| !! | AGAAAGTGTTGTGTTAGCTA+TGG | - | chr1.4:85622086-85622105 | MS.gene029349:CDS | 35.0% |
| AGTCTCATGGTGTGAAAGAT+TGG | - | chr1.4:85622160-85622179 | MS.gene029349:CDS | 40.0% | |
| ATTGGTACGAGGAGTTAAGT+CGG | - | chr1.4:85621990-85622009 | MS.gene029349:CDS | 40.0% | |
| CACGAGTTCGATCTTAGAAA+AGG | - | chr1.4:85622038-85622057 | MS.gene029349:CDS | 40.0% | |
| GTCTCATGGTGTGAAAGATT+GGG | - | chr1.4:85622161-85622180 | MS.gene029349:CDS | 40.0% | |
| TGAGAAACTTGCAGATTCAG+AGG | + | chr1.4:85621920-85621939 | None:intergenic | 40.0% | |
| TGTCCCTCACATAGATACAA+AGG | + | chr1.4:85622366-85622385 | None:intergenic | 40.0% | |
| TGTGAAAGATTGGGAAGGTT+TGG | - | chr1.4:85622170-85622189 | MS.gene029349:CDS | 40.0% | |
| TTAGAAAAGGCGAAAGCGAA+TGG | - | chr1.4:85622051-85622070 | MS.gene029349:CDS | 40.0% | |
| !!! | ACTCTTTCTTTTCGAGTTGG+TGG | - | chr1.4:85621799-85621818 | MS.gene029349:intron | 40.0% |
| CAAGAGGGTAAGAATTGCCA+TGG | + | chr1.4:85621766-85621785 | None:intergenic | 45.0% | |
| CATGGTGTGAAAGATTGGGA+AGG | - | chr1.4:85622165-85622184 | MS.gene029349:CDS | 45.0% | |
| GAAGAGATGGTTGAGTCTCA+TGG | - | chr1.4:85622147-85622166 | MS.gene029349:CDS | 45.0% | |
| TCATTGGAGACATTGGTACG+AGG | - | chr1.4:85621979-85621998 | MS.gene029349:CDS | 45.0% | |
| TGGCAATTCTTACCCTCTTG+TGG | - | chr1.4:85621766-85621785 | MS.gene029349:intron | 45.0% | |
| TGTGTTAGCTATGGAGTCAG+AGG | - | chr1.4:85622095-85622114 | MS.gene029349:CDS | 45.0% | |
| ! | GTTTGGAAGAGCTTTTGAGC+TGG | - | chr1.4:85622187-85622206 | MS.gene029349:CDS | 45.0% |
| !! | AGTTTTTGGATGGCCACAAG+AGG | + | chr1.4:85621782-85621801 | None:intergenic | 45.0% |
| !! | GTTTTTGGATGGCCACAAGA+GGG | + | chr1.4:85621781-85621800 | None:intergenic | 45.0% |
| CGGCGAATCATTGGAGACAT+TGG | - | chr1.4:85621972-85621991 | MS.gene029349:CDS | 50.0% | |
| CTACAACGACGGCGAATCAT+TGG | - | chr1.4:85621963-85621982 | MS.gene029349:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 85621694 | 85622496 | 85621694 | ID=MS.gene029349 |
| chr1.4 | mRNA | 85621694 | 85622496 | 85621694 | ID=MS.gene029349.t1;Parent=MS.gene029349 |
| chr1.4 | exon | 85621965 | 85622496 | 85621965 | ID=MS.gene029349.t1.exon1;Parent=MS.gene029349.t1 |
| chr1.4 | CDS | 85621965 | 85622496 | 85621965 | ID=cds.MS.gene029349.t1;Parent=MS.gene029349.t1 |
| chr1.4 | exon | 85621694 | 85621698 | 85621694 | ID=MS.gene029349.t1.exon2;Parent=MS.gene029349.t1 |
| chr1.4 | CDS | 85621694 | 85621698 | 85621694 | ID=cds.MS.gene029349.t1;Parent=MS.gene029349.t1 |
| Gene Sequence |
| Protein sequence |
