AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02935

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02935.t1 MTR_2g026250 90.516 601 49 4 1 596 1 598 0.0 1115
MS.gene02935.t1 MTR_4g133660 55.132 604 202 8 1 595 1 544 0.0 620
MS.gene02935.t1 MTR_5g097480 47.769 605 244 13 1 594 1 544 0.0 529
MS.gene02935.t1 MTR_3g056110 54.588 425 189 3 173 594 119 542 4.50e-170 494
MS.gene02935.t1 MTR_5g094450 56.959 388 162 3 211 594 146 532 4.09e-160 469
MS.gene02935.t1 MTR_2g082090 54.712 382 166 2 215 593 201 578 4.21e-148 439
MS.gene02935.t1 MTR_1g029420 46.900 371 196 1 224 594 223 592 2.73e-126 384
MS.gene02935.t1 MTR_3g089055 46.900 371 196 1 224 594 201 570 9.49e-123 374
MS.gene02935.t1 MTR_3g089055 46.900 371 196 1 224 594 196 565 1.12e-122 374
MS.gene02935.t1 MTR_7g027190 34.596 396 222 6 221 595 295 674 2.90e-71 242
MS.gene02935.t1 MTR_4g064160 32.275 378 246 3 221 593 311 683 8.22e-64 222
MS.gene02935.t1 MTR_4g064200 32.804 378 242 6 221 593 279 649 1.24e-62 218
MS.gene02935.t1 MTR_4g064150 30.688 378 256 4 221 593 356 732 8.75e-62 218
MS.gene02935.t1 MTR_7g062120 30.400 375 259 2 221 594 367 740 1.07e-60 215
MS.gene02935.t1 MTR_7g057230 33.777 376 232 6 224 593 292 656 4.28e-59 209
MS.gene02935.t1 MTR_2g097310 32.095 377 246 6 221 593 267 637 5.54e-59 208
MS.gene02935.t1 MTR_2g097390 30.851 376 252 4 221 593 270 640 6.06e-59 208
MS.gene02935.t1 MTR_6g047750 33.333 393 217 9 224 594 293 662 1.05e-58 208
MS.gene02935.t1 MTR_2g097463 29.947 374 257 3 221 593 285 654 4.41e-56 201
MS.gene02935.t1 MTR_2g097467 29.708 377 260 4 218 593 281 653 6.69e-56 200
MS.gene02935.t1 MTR_2g097350 31.481 378 247 6 221 593 269 639 1.03e-55 199
MS.gene02935.t1 MTR_2g097473 30.933 375 252 6 221 593 284 653 1.21e-55 199
MS.gene02935.t1 MTR_2g097410 29.894 378 257 5 221 593 366 740 1.63e-55 200
MS.gene02935.t1 MTR_4g064120 29.211 380 255 5 221 591 249 623 1.83e-55 198
MS.gene02935.t1 MTR_4g064180 28.947 380 256 5 221 591 249 623 6.08e-54 194
MS.gene02935.t1 MTR_3g065980 31.218 394 229 11 223 595 169 541 3.11e-53 191
MS.gene02935.t1 MTR_4g104020 31.701 388 242 6 221 595 144 521 5.48e-53 189
MS.gene02935.t1 MTR_7g074650 32.425 367 232 7 229 594 441 792 2.10e-51 189
MS.gene02935.t1 MTR_8g442410 30.287 383 251 6 221 594 150 525 6.34e-49 178
MS.gene02935.t1 MTR_4g097080 29.379 354 237 5 249 594 155 503 8.76e-49 177
MS.gene02935.t1 MTR_3g022830 27.231 437 236 10 217 594 23 436 6.14e-48 174
MS.gene02935.t1 MTR_2g097380 28.818 347 215 4 248 593 245 560 5.02e-45 168
MS.gene02935.t1 MTR_4g102790 31.461 356 223 9 250 593 315 661 3.21e-44 167
MS.gene02935.t1 MTR_5g015490 29.282 362 233 8 249 594 136 490 7.18e-44 164
MS.gene02935.t1 MTR_4g077760 31.161 353 222 10 250 594 216 555 4.35e-40 154
MS.gene02935.t1 MTR_1g096030 30.688 378 208 14 250 594 103 459 2.26e-39 150
MS.gene02935.t1 MTR_4g076020 29.363 361 218 11 243 594 97 429 4.44e-37 143
MS.gene02935.t1 MTR_2g034250 26.357 387 266 7 221 594 205 585 1.52e-36 144
MS.gene02935.t1 MTR_3g072710 27.778 360 225 10 258 595 155 501 5.15e-36 142
MS.gene02935.t1 MTR_2g034280 25.714 385 268 9 221 594 198 575 1.59e-34 138
MS.gene02935.t1 MTR_2g034260 25.974 385 265 9 223 594 207 584 1.60e-34 138
MS.gene02935.t1 MTR_1g069725 28.994 338 218 9 262 595 145 464 6.00e-34 135
MS.gene02935.t1 MTR_5g058860 27.654 358 233 12 255 595 150 498 7.42e-33 132
MS.gene02935.t1 MTR_7g109580 27.089 347 237 8 255 594 217 554 1.25e-32 132
MS.gene02935.t1 MTR_2g099110 27.062 388 252 9 227 594 75 451 7.02e-32 129
MS.gene02935.t1 MTR_2g089100 26.055 403 257 11 223 593 49 442 1.42e-31 128
MS.gene02935.t1 MTR_4g026485 25.858 379 252 11 225 595 268 625 2.00e-28 120
MS.gene02935.t1 MTR_1g086970 25.143 350 239 8 258 594 139 478 6.95e-28 117
MS.gene02935.t1 MTR_5g015950 26.168 321 201 6 307 593 127 445 1.58e-27 116
MS.gene02935.t1 MTR_3g053270 26.168 321 201 6 307 593 127 445 1.58e-27 116
MS.gene02935.t1 MTR_4g095500 24.940 417 262 9 223 593 56 467 2.68e-27 115
MS.gene02935.t1 MTR_5g009080 31.050 219 138 5 227 440 57 267 1.64e-25 110
MS.gene02935.t1 MTR_5g009080 35.915 142 90 1 455 595 338 479 1.74e-22 101
MS.gene02935.t1 MTR_8g093070 27.042 355 217 16 254 593 168 495 3.65e-24 106
MS.gene02935.t1 MTR_4g076140 39.007 141 85 1 455 594 329 469 5.68e-24 105
MS.gene02935.t1 MTR_4g076140 29.952 207 137 3 227 432 49 248 5.40e-23 102
MS.gene02935.t1 MTR_0092s0100 25.330 379 255 11 225 595 374 732 8.36e-24 106
MS.gene02935.t1 MTR_0092s0100 25.330 379 255 11 225 595 371 729 9.83e-24 106
MS.gene02935.t1 MTR_8g020840 24.688 401 250 15 225 594 169 548 3.29e-22 100
MS.gene02935.t1 MTR_3g022580 30.000 110 77 0 485 594 74 183 6.02e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02935.t1 AT4G17230 47.167 600 236 10 1 594 1 525 6.14e-179 516
MS.gene02935.t1 AT4G17230 47.167 600 236 10 1 594 1 525 6.14e-179 516
MS.gene02935.t1 AT5G48150 55.581 430 186 4 167 594 64 490 3.21e-169 490
MS.gene02935.t1 AT5G48150 55.581 430 186 4 167 594 64 490 3.21e-169 490
MS.gene02935.t1 AT5G48150 55.581 430 186 4 167 594 64 490 3.21e-169 490
MS.gene02935.t1 AT5G48150 55.581 430 186 4 167 594 64 490 3.21e-169 490
MS.gene02935.t1 AT1G50600 52.349 447 202 5 150 594 89 526 3.02e-160 469
MS.gene02935.t1 AT2G04890 57.662 385 155 2 210 594 37 413 4.32e-160 464
MS.gene02935.t1 AT1G50600 54.028 422 189 3 174 594 180 597 1.12e-159 470
MS.gene02935.t1 AT1G50600 54.028 422 189 3 174 594 184 601 1.50e-159 470
MS.gene02935.t1 AT1G21450 45.526 380 205 2 216 594 215 593 1.44e-120 369
MS.gene02935.t1 AT1G07530 31.746 378 247 4 222 593 392 764 1.20e-61 218
MS.gene02935.t1 AT3G03450 33.245 376 237 9 223 594 180 545 1.30e-60 211
MS.gene02935.t1 AT5G52510 33.908 348 205 7 262 594 303 640 1.31e-59 210
MS.gene02935.t1 AT5G59450 30.423 378 258 4 221 594 222 598 3.46e-59 208
MS.gene02935.t1 AT2G37650 31.135 379 249 4 221 594 342 713 7.15e-59 209
MS.gene02935.t1 AT2G37650 31.135 379 249 4 221 594 330 701 8.17e-59 209
MS.gene02935.t1 AT5G17490 30.303 495 303 16 124 594 40 516 1.81e-58 204
MS.gene02935.t1 AT3G46600 30.563 373 256 3 223 594 1 371 8.85e-58 198
MS.gene02935.t1 AT3G46600 30.667 375 257 3 221 594 175 547 1.18e-57 203
MS.gene02935.t1 AT3G46600 30.667 375 257 3 221 594 207 579 1.39e-57 203
MS.gene02935.t1 AT2G29060 30.789 380 250 6 222 593 280 654 7.43e-57 203
MS.gene02935.t1 AT1G14920 31.593 383 229 10 223 594 169 529 7.48e-57 200
MS.gene02935.t1 AT2G29060 30.789 380 250 6 222 593 317 691 1.15e-56 203
MS.gene02935.t1 AT2G29065 30.628 382 255 7 221 593 247 627 3.07e-54 195
MS.gene02935.t1 AT1G66350 31.579 380 229 12 223 595 152 507 3.91e-54 192
MS.gene02935.t1 AT2G01570 31.414 382 231 11 223 594 221 581 4.67e-54 194
MS.gene02935.t1 AT1G50420 28.837 430 238 8 227 595 58 480 5.19e-54 191
MS.gene02935.t1 AT1G07520 30.366 382 255 8 221 593 149 528 2.56e-51 185
MS.gene02935.t1 AT1G07520 30.366 382 255 8 221 593 149 528 2.56e-51 185
MS.gene02935.t1 AT1G07520 29.659 381 259 6 221 593 314 693 1.18e-50 186
MS.gene02935.t1 AT1G07520 29.659 381 259 6 221 593 313 692 1.27e-50 186
MS.gene02935.t1 AT3G54220 31.165 369 235 8 229 593 296 649 1.61e-46 174
MS.gene02935.t1 AT5G66770 33.811 349 213 7 254 594 246 584 7.48e-45 168
MS.gene02935.t1 AT3G50650 32.286 350 219 7 254 594 202 542 1.66e-43 164
MS.gene02935.t1 AT4G37650 29.820 389 246 10 227 594 147 529 8.93e-42 158
MS.gene02935.t1 AT1G63100 28.535 389 249 7 226 593 273 653 1.58e-41 159
MS.gene02935.t1 AT1G63100 28.535 389 249 7 226 593 248 628 1.87e-41 159
MS.gene02935.t1 AT5G41920 27.926 376 244 10 227 594 44 400 1.21e-40 152
MS.gene02935.t1 AT1G55580 26.129 310 197 7 304 594 149 445 6.81e-30 123
MS.gene02935.t1 AT4G08250 25.926 351 247 7 250 593 132 476 3.28e-29 121
MS.gene02935.t1 AT3G49950 23.967 363 251 10 249 593 52 407 6.56e-24 104
MS.gene02935.t1 AT3G60630 24.716 352 230 10 260 595 291 623 9.91e-23 103
MS.gene02935.t1 AT2G45160 25.978 358 215 13 259 595 312 640 2.10e-21 99.0
MS.gene02935.t1 AT4G00150 23.873 377 257 11 223 595 205 555 9.77e-20 93.6
MS.gene02935.t1 AT3G13840 23.464 358 245 10 249 594 170 510 6.86e-19 90.5
MS.gene02935.t1 AT4G36710 23.969 388 259 12 221 594 120 485 3.69e-18 88.2
MS.gene02935.t1 AT5G67411 29.101 189 121 5 352 531 14 198 1.85e-12 67.0

Find 110 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTAAGCAAACTGAAAATA+TGG 0.186262 2.4:-62326789 None:intergenic
AAGATTTCAATAAAGATTAT+AGG 0.216624 2.4:+62327552 MS.gene02935:CDS
TCAAAGAACCAATGTTTCTT+TGG 0.242227 2.4:+62325999 MS.gene02935:CDS
CCTCCATTCGTTCGTGTAAC+AGG 0.262317 2.4:+62326939 MS.gene02935:CDS
TGAGAAAATTGTGGTGAAAA+AGG 0.281191 2.4:-62326138 None:intergenic
AAAGCTCAATTTCTTTCATT+TGG 0.293718 2.4:-62326242 None:intergenic
ACTGTGATCCTTGTGCAATT+TGG 0.334053 2.4:-62326875 None:intergenic
GATTTCAGAGCTAGGCTATA+AGG 0.338912 2.4:+62326374 MS.gene02935:CDS
CTCATCAACTCATTGCTTGT+TGG 0.342988 2.4:-62326738 None:intergenic
TTGCAAATCAATTCAAATAA+TGG 0.345919 2.4:+62325967 MS.gene02935:CDS
GCTGTCGATGATATCTGAAT+TGG 0.360875 2.4:-62326421 None:intergenic
TAAGCACCTAACCTTTGAAT+TGG 0.365266 2.4:-62326642 None:intergenic
TCTTCTTTCAAGTCTAGCTT+TGG 0.368681 2.4:-62326519 None:intergenic
GTTGAAAGACATGAACTGTT+TGG 0.373187 2.4:+62327443 MS.gene02935:CDS
TTAACACTATTAAACTCAAA+TGG 0.374352 2.4:-62327053 None:intergenic
TTTAATAGTGTTAAAATGTA+TGG 0.383878 2.4:+62327062 MS.gene02935:CDS
AATTTGAGACCAATCATATT+CGG 0.388708 2.4:-62326484 None:intergenic
TGTGCACAGTTTGTATTTGA+TGG 0.403494 2.4:+62326552 MS.gene02935:CDS
TTTCTTTGGAAACAAGCAAC+TGG 0.406710 2.4:+62326013 MS.gene02935:CDS
GAAAGAAATTGAGCTTTCAT+TGG 0.409492 2.4:+62326248 MS.gene02935:CDS
TCTGTCTCAAAGACTGTTGC+TGG 0.413091 2.4:-62325915 None:intergenic
GAATCGGAGATTTCATTGTT+AGG 0.417405 2.4:+62326396 MS.gene02935:CDS
AGAGCAAGGGTGGAATCATC+AGG 0.423843 2.4:+62326681 MS.gene02935:CDS
GTATGGATGTGAGGTTCAAC+TGG 0.437985 2.4:+62327079 MS.gene02935:CDS
ATCAAAAGCTATGAATTCAT+TGG 0.443080 2.4:-62326073 None:intergenic
AGCTGCCAGTCCAACTTAAA+CGG 0.448434 2.4:+62326441 MS.gene02935:CDS
GGTTCTCCAATTCAAAGGTT+AGG 0.459338 2.4:+62326636 MS.gene02935:CDS
GAAATTGAGCTTTCATTGGT+AGG 0.460607 2.4:+62326252 MS.gene02935:CDS
GCCTAAGGTTGTGTTATTTG+TGG 0.466645 2.4:+62327232 MS.gene02935:CDS
GGTGGAAGACTTGATATTGT+AGG 0.469286 2.4:+62326993 MS.gene02935:CDS
GGTACCGATATAGAAGTATT+CGG 0.469425 2.4:+62326273 MS.gene02935:CDS
TGTAAGCAAACTGAAAATAT+GGG 0.469553 2.4:-62326788 None:intergenic
ACCTCTAGCATGAAACGATT+GGG 0.473069 2.4:-62326973 None:intergenic
TGCTTCACAAATGACAGCAT+TGG 0.474342 2.4:-62326817 None:intergenic
CACCTCTAGCATGAAACGAT+TGG 0.475376 2.4:-62326974 None:intergenic
GAATAACGTGTTGGGAAAGA+TGG 0.476145 2.4:+62326602 MS.gene02935:CDS
GCTGCCGAATACTTCTATAT+CGG 0.477399 2.4:-62326277 None:intergenic
TCCATGCTCACGCTCTCGTC+CGG 0.480063 2.4:-62327167 None:intergenic
AAGGTACATCCGAATATGAT+TGG 0.489420 2.4:+62326475 MS.gene02935:CDS
GTGAAGGCTACCATTTGAGT+TGG 0.490964 2.4:-62326307 None:intergenic
TTATGTGCACATCTTCTGCT+TGG 0.493993 2.4:+62327621 MS.gene02935:CDS
CTGTCGATGATATCTGAATT+GGG 0.497389 2.4:-62326420 None:intergenic
AGCTGTCTGTCCAACTCAAA+TGG 0.502650 2.4:+62326297 MS.gene02935:CDS
GGAAAGATGGTATCGGTTGG+CGG 0.502999 2.4:+62326615 MS.gene02935:CDS
GGATTCATGAATAACGTGTT+GGG 0.509988 2.4:+62326594 MS.gene02935:CDS
AGGATTCATGAATAACGTGT+TGG 0.512384 2.4:+62326593 MS.gene02935:CDS
AGAGCTAGGCTATAAGGAAT+CGG 0.514017 2.4:+62326380 MS.gene02935:CDS
GGCTACCGTTTAAGTTGGAC+TGG 0.514240 2.4:-62326446 None:intergenic
GTGGAGGCTACCGTTTAAGT+TGG 0.523399 2.4:-62326451 None:intergenic
ATTCTCTTCTTATCATCTCT+AGG 0.523465 2.4:-62327353 None:intergenic
GATTCTCCTTCATCTTCTAG+TGG 0.524436 2.4:+62326093 MS.gene02935:CDS
CATTATCTTGAGATGTACCT+TGG 0.526204 2.4:-62326329 None:intergenic
TCAGTCACCGAGGGACTCAA+TGG 0.526464 2.4:-62327509 None:intergenic
GACATGAACTGTTTGGAAAG+TGG 0.527553 2.4:+62327450 MS.gene02935:CDS
CGAACGAATGGAGGTCCGCC+AGG 0.528325 2.4:-62326930 None:intergenic
CAAAAGAAACGTAGTTCAGC+CGG 0.533280 2.4:+62325877 MS.gene02935:CDS
GCTTGTTTCCAAAGAAACAT+TGG 0.537666 2.4:-62326007 None:intergenic
GTTAGGTGCTTACATGTTGG+AGG 0.539755 2.4:+62326653 MS.gene02935:CDS
CTTAAAGTCTCAGCAAACCT+TGG 0.540575 2.4:-62327290 None:intergenic
GGTATGTGATGCAAAGCAAA+AGG 0.541821 2.4:-62327146 None:intergenic
GACTGTTGCTGGTAGTATAA+CGG 0.543849 2.4:-62325904 None:intergenic
CATGCTCTCAAGCATAAACC+TGG 0.546708 2.4:+62326912 MS.gene02935:CDS
ACCGTTACCATTGAGTCCCT+CGG 0.546824 2.4:+62327502 MS.gene02935:CDS
AAGGTTAGGTGCTTACATGT+TGG 0.546985 2.4:+62326650 MS.gene02935:CDS
TTGGCGGTTCTCCAATTCAA+AGG 0.547368 2.4:+62326631 MS.gene02935:CDS
GCATGGAGAATCATAGAGAC+AGG 0.548705 2.4:+62327183 MS.gene02935:CDS
TTCTTTGGAAACAAGCAACT+GGG 0.548975 2.4:+62326014 MS.gene02935:CDS
AACACAAATGTATCGCCGTT+AGG 0.550761 2.4:+62326177 MS.gene02935:CDS
CTGGTAGTATAACGGTATAC+CGG 0.553841 2.4:-62325896 None:intergenic
ACCGGACGAGAGCGTGAGCA+TGG 0.554808 2.4:+62327166 MS.gene02935:CDS
TCCACAAATAACACAACCTT+AGG 0.558882 2.4:-62327233 None:intergenic
CTTGAGATGTACCTTGGTGA+AGG 0.559050 2.4:-62326323 None:intergenic
TCCCAATCGTTTCATGCTAG+AGG 0.561436 2.4:+62326972 MS.gene02935:CDS
GGAGGGATTAAGAGCAAGGG+TGG 0.565223 2.4:+62326671 MS.gene02935:CDS
GAGTCATAGTGAGAAAATTG+TGG 0.570992 2.4:-62326147 None:intergenic
CATATTCGGATGTACCTTGG+TGG 0.571085 2.4:-62326470 None:intergenic
CAATCGTTTCATGCTAGAGG+TGG 0.577074 2.4:+62326975 MS.gene02935:CDS
AATCATATTCGGATGTACCT+TGG 0.577241 2.4:-62326473 None:intergenic
TGTTGGAGGGATTAAGAGCA+AGG 0.578699 2.4:+62326667 MS.gene02935:CDS
GAATTAATGATTTCAGAGCT+AGG 0.580583 2.4:+62326366 MS.gene02935:CDS
TTAGGTGCTTACATGTTGGA+GGG 0.580819 2.4:+62326654 MS.gene02935:CDS
GCATTACCACTAGAAGATGA+AGG 0.589681 2.4:-62326099 None:intergenic
GTGGTAATGCATCCAACACC+AGG 0.589942 2.4:+62326112 MS.gene02935:CDS
CGTTAACATCATAGCTTGTG+AGG 0.593625 2.4:+62327409 MS.gene02935:CDS
ATTCGGATGTACCTTGGTGG+AGG 0.599110 2.4:-62326467 None:intergenic
CGTGTTGGGAAAGATGGTAT+CGG 0.599692 2.4:+62326608 MS.gene02935:CDS
TTGGGAAAGATGGTATCGGT+TGG 0.601702 2.4:+62326612 MS.gene02935:CDS
CGATACATTTGTGTTATCAG+AGG 0.611074 2.4:-62326169 None:intergenic
GGCTACTTGAGAAGATTCCA+AGG 0.613501 2.4:-62326052 None:intergenic
ATACCTGTTACACGAACGAA+TGG 0.616579 2.4:-62326942 None:intergenic
GTTAACATCATAGCTTGTGA+GGG 0.618537 2.4:+62327410 MS.gene02935:CDS
ACCGAGGGACTCAATGGTAA+CGG 0.634336 2.4:-62327503 None:intergenic
CCTGTTACACGAACGAATGG+AGG 0.644699 2.4:-62326939 None:intergenic
GCACTAGAGTAAACACCTAA+CGG 0.650576 2.4:-62326192 None:intergenic
ATTGACTTCCAAATTGCACA+AGG 0.655189 2.4:+62326867 MS.gene02935:CDS
GTTAGGTGTTTACTCTAGTG+CGG 0.655711 2.4:+62326194 MS.gene02935:CDS
ATGAACTGTTTGGAAAGTGG+AGG 0.656082 2.4:+62327453 MS.gene02935:CDS
TGTTAAAATGTATGGATGTG+AGG 0.656156 2.4:+62327070 MS.gene02935:CDS
GTTGGAGGGATTAAGAGCAA+GGG 0.658974 2.4:+62326668 MS.gene02935:CDS
GAGCAAGGGTGGAATCATCA+GGG 0.659667 2.4:+62326682 MS.gene02935:CDS
ATGTTGCTATCAATTTAGCG+TGG 0.662028 2.4:+62327588 MS.gene02935:CDS
AATGTTTGAATCAATTGATG+TGG 0.662206 2.4:+62327325 MS.gene02935:CDS
CTAACTGAATCAGTCACCGA+GGG 0.680621 2.4:-62327518 None:intergenic
GTTTACTCTAGTGCGGACGA+TGG 0.696726 2.4:+62326201 MS.gene02935:CDS
TAATGCAGAACAACACTGTG+TGG 0.712966 2.4:+62327376 MS.gene02935:CDS
GCTCTCAAGCATAAACCTGG+CGG 0.733186 2.4:+62326915 MS.gene02935:CDS
TCAAATGGTAGCCTTCACCA+AGG 0.752690 2.4:+62326312 MS.gene02935:CDS
TCTAACTGAATCAGTCACCG+AGG 0.753321 2.4:-62327519 None:intergenic
TTAAACGGTAGCCTCCACCA+AGG 0.778392 2.4:+62326456 MS.gene02935:CDS
AAATTGCACAAGGATCACAG+TGG 0.820097 2.4:+62326877 MS.gene02935:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTTCAATAAAGATTAT+AGG + chr2.4:62327552-62327571 MS.gene02935:CDS 15.0%
!! TTTAATAGTGTTAAAATGTA+TGG + chr2.4:62327062-62327081 MS.gene02935:CDS 15.0%
!! TTAACACTATTAAACTCAAA+TGG - chr2.4:62327056-62327075 None:intergenic 20.0%
!! TTGCAAATCAATTCAAATAA+TGG + chr2.4:62325967-62325986 MS.gene02935:CDS 20.0%
! AAAGCTCAATTTCTTTCATT+TGG - chr2.4:62326245-62326264 None:intergenic 25.0%
! AATGTTTGAATCAATTGATG+TGG + chr2.4:62327325-62327344 MS.gene02935:CDS 25.0%
! ATCAAAAGCTATGAATTCAT+TGG - chr2.4:62326076-62326095 None:intergenic 25.0%
! ATGTAAGCAAACTGAAAATA+TGG - chr2.4:62326792-62326811 None:intergenic 25.0%
! TGTAAGCAAACTGAAAATAT+GGG - chr2.4:62326791-62326810 None:intergenic 25.0%
!! AATTTGAGACCAATCATATT+CGG - chr2.4:62326487-62326506 None:intergenic 25.0%
ATTCTCTTCTTATCATCTCT+AGG - chr2.4:62327356-62327375 None:intergenic 30.0%
GAAAGAAATTGAGCTTTCAT+TGG + chr2.4:62326248-62326267 MS.gene02935:CDS 30.0%
GAATTAATGATTTCAGAGCT+AGG + chr2.4:62326366-62326385 MS.gene02935:CDS 30.0%
TCAAAGAACCAATGTTTCTT+TGG + chr2.4:62325999-62326018 MS.gene02935:CDS 30.0%
TGAGAAAATTGTGGTGAAAA+AGG - chr2.4:62326141-62326160 None:intergenic 30.0%
TGTTAAAATGTATGGATGTG+AGG + chr2.4:62327070-62327089 MS.gene02935:CDS 30.0%
! GGTGATTTTCAAAAAGCAAT+AGG + chr2.4:62326573-62326592 MS.gene02935:CDS 30.0%
AAGGTACATCCGAATATGAT+TGG + chr2.4:62326475-62326494 MS.gene02935:CDS 35.0%
AATCATATTCGGATGTACCT+TGG - chr2.4:62326476-62326495 None:intergenic 35.0%
AGGATTCATGAATAACGTGT+TGG + chr2.4:62326593-62326612 MS.gene02935:CDS 35.0%
ATGTTGCTATCAATTTAGCG+TGG + chr2.4:62327588-62327607 MS.gene02935:CDS 35.0%
CATTATCTTGAGATGTACCT+TGG - chr2.4:62326332-62326351 None:intergenic 35.0%
CGATACATTTGTGTTATCAG+AGG - chr2.4:62326172-62326191 None:intergenic 35.0%
CTGTCGATGATATCTGAATT+GGG - chr2.4:62326423-62326442 None:intergenic 35.0%
GAAATTGAGCTTTCATTGGT+AGG + chr2.4:62326252-62326271 MS.gene02935:CDS 35.0%
GAATCGGAGATTTCATTGTT+AGG + chr2.4:62326396-62326415 MS.gene02935:CDS 35.0%
GAGTCATAGTGAGAAAATTG+TGG - chr2.4:62326150-62326169 None:intergenic 35.0%
GCTTGTTTCCAAAGAAACAT+TGG - chr2.4:62326010-62326029 None:intergenic 35.0%
GGATTCATGAATAACGTGTT+GGG + chr2.4:62326594-62326613 MS.gene02935:CDS 35.0%
GTTGAAAGACATGAACTGTT+TGG + chr2.4:62327443-62327462 MS.gene02935:CDS 35.0%
TAAGCACCTAACCTTTGAAT+TGG - chr2.4:62326645-62326664 None:intergenic 35.0%
TCAGCAAACCTTGGTAAAAA+TGG - chr2.4:62327284-62327303 None:intergenic 35.0%
TCCACAAATAACACAACCTT+AGG - chr2.4:62327236-62327255 None:intergenic 35.0%
TGTGCACAGTTTGTATTTGA+TGG + chr2.4:62326552-62326571 MS.gene02935:CDS 35.0%
! AGGAAAAGATTTTCAATGGC+GGG + chr2.4:62327473-62327492 MS.gene02935:CDS 35.0%
! ATTGACTTCCAAATTGCACA+AGG + chr2.4:62326867-62326886 MS.gene02935:CDS 35.0%
! GAGGAAAAGATTTTCAATGG+CGG + chr2.4:62327472-62327491 MS.gene02935:CDS 35.0%
! GTGGAGGAAAAGATTTTCAA+TGG + chr2.4:62327469-62327488 MS.gene02935:CDS 35.0%
! GTTAACATCATAGCTTGTGA+GGG + chr2.4:62327410-62327429 MS.gene02935:CDS 35.0%
! TCTTCTTTCAAGTCTAGCTT+TGG - chr2.4:62326522-62326541 None:intergenic 35.0%
! TTCTTTGGAAACAAGCAACT+GGG + chr2.4:62326014-62326033 MS.gene02935:CDS 35.0%
! TTTCTTTGGAAACAAGCAAC+TGG + chr2.4:62326013-62326032 MS.gene02935:CDS 35.0%
! TTTTGCTTTGCATCACATAC+CGG + chr2.4:62327148-62327167 MS.gene02935:CDS 35.0%
!! ACACTTCTCCATTTTTACCA+AGG + chr2.4:62327273-62327292 MS.gene02935:CDS 35.0%
!! GGTACCGATATAGAAGTATT+CGG + chr2.4:62326273-62326292 MS.gene02935:CDS 35.0%
AAATTGCACAAGGATCACAG+TGG + chr2.4:62326877-62326896 MS.gene02935:CDS 40.0%
AACACAAATGTATCGCCGTT+AGG + chr2.4:62326177-62326196 MS.gene02935:CDS 40.0%
AAGGTTAGGTGCTTACATGT+TGG + chr2.4:62326650-62326669 MS.gene02935:CDS 40.0%
ACCTCTAGCATGAAACGATT+GGG - chr2.4:62326976-62326995 None:intergenic 40.0%
ACTGTGATCCTTGTGCAATT+TGG - chr2.4:62326878-62326897 None:intergenic 40.0%
AGAGCTAGGCTATAAGGAAT+CGG + chr2.4:62326380-62326399 MS.gene02935:CDS 40.0%
ATACCTGTTACACGAACGAA+TGG - chr2.4:62326945-62326964 None:intergenic 40.0%
ATGAACTGTTTGGAAAGTGG+AGG + chr2.4:62327453-62327472 MS.gene02935:CDS 40.0%
CAAAAGAAACGTAGTTCAGC+CGG + chr2.4:62325877-62325896 MS.gene02935:CDS 40.0%
CTCATCAACTCATTGCTTGT+TGG - chr2.4:62326741-62326760 None:intergenic 40.0%
CTGGTAGTATAACGGTATAC+CGG - chr2.4:62325899-62325918 None:intergenic 40.0%
CTTAAAGTCTCAGCAAACCT+TGG - chr2.4:62327293-62327312 None:intergenic 40.0%
GAATAACGTGTTGGGAAAGA+TGG + chr2.4:62326602-62326621 MS.gene02935:CDS 40.0%
GACATGAACTGTTTGGAAAG+TGG + chr2.4:62327450-62327469 MS.gene02935:CDS 40.0%
GACTGTTGCTGGTAGTATAA+CGG - chr2.4:62325907-62325926 None:intergenic 40.0%
GATTCTCCTTCATCTTCTAG+TGG + chr2.4:62326093-62326112 MS.gene02935:CDS 40.0%
GATTTCAGAGCTAGGCTATA+AGG + chr2.4:62326374-62326393 MS.gene02935:CDS 40.0%
GCACTAGAGTAAACACCTAA+CGG - chr2.4:62326195-62326214 None:intergenic 40.0%
GCATTACCACTAGAAGATGA+AGG - chr2.4:62326102-62326121 None:intergenic 40.0%
GCTGCCGAATACTTCTATAT+CGG - chr2.4:62326280-62326299 None:intergenic 40.0%
GCTGTCGATGATATCTGAAT+TGG - chr2.4:62326424-62326443 None:intergenic 40.0%
GGTATGTGATGCAAAGCAAA+AGG - chr2.4:62327149-62327168 None:intergenic 40.0%
GGTGGAAGACTTGATATTGT+AGG + chr2.4:62326993-62327012 MS.gene02935:CDS 40.0%
GGTTCTCCAATTCAAAGGTT+AGG + chr2.4:62326636-62326655 MS.gene02935:CDS 40.0%
GTTAGGTGTTTACTCTAGTG+CGG + chr2.4:62326194-62326213 MS.gene02935:CDS 40.0%
TAATGCAGAACAACACTGTG+TGG + chr2.4:62327376-62327395 MS.gene02935:CDS 40.0%
TGAAAAAGGATTCCTGGTGT+TGG - chr2.4:62326127-62326146 None:intergenic 40.0%
TGCTTCACAAATGACAGCAT+TGG - chr2.4:62326820-62326839 None:intergenic 40.0%
TTAGGTGCTTACATGTTGGA+GGG + chr2.4:62326654-62326673 MS.gene02935:CDS 40.0%
TTATGTGCACATCTTCTGCT+TGG + chr2.4:62327621-62327640 MS.gene02935:CDS 40.0%
TTGTGGTGAAAAAGGATTCC+TGG - chr2.4:62326133-62326152 None:intergenic 40.0%
! CGTTAACATCATAGCTTGTG+AGG + chr2.4:62327409-62327428 MS.gene02935:CDS 40.0%
! GGAGAAGCAATTTTGTACCT+TGG + chr2.4:62326035-62326054 MS.gene02935:CDS 40.0%
!! GCCTAAGGTTGTGTTATTTG+TGG + chr2.4:62327232-62327251 MS.gene02935:CDS 40.0%
AGCTGCCAGTCCAACTTAAA+CGG + chr2.4:62326441-62326460 MS.gene02935:CDS 45.0%
AGCTGTCTGTCCAACTCAAA+TGG + chr2.4:62326297-62326316 MS.gene02935:CDS 45.0%
CAATCGTTTCATGCTAGAGG+TGG + chr2.4:62326975-62326994 MS.gene02935:CDS 45.0%
CACCTCTAGCATGAAACGAT+TGG - chr2.4:62326977-62326996 None:intergenic 45.0%
CATATTCGGATGTACCTTGG+TGG - chr2.4:62326473-62326492 None:intergenic 45.0%
CATGCTCTCAAGCATAAACC+TGG + chr2.4:62326912-62326931 MS.gene02935:CDS 45.0%
CGTGTTGGGAAAGATGGTAT+CGG + chr2.4:62326608-62326627 MS.gene02935:CDS 45.0%
CTAACTGAATCAGTCACCGA+GGG - chr2.4:62327521-62327540 None:intergenic 45.0%
CTTGAGATGTACCTTGGTGA+AGG - chr2.4:62326326-62326345 None:intergenic 45.0%
GCATGGAGAATCATAGAGAC+AGG + chr2.4:62327183-62327202 MS.gene02935:CDS 45.0%
GGCTACTTGAGAAGATTCCA+AGG - chr2.4:62326055-62326074 None:intergenic 45.0%
GTATGGATGTGAGGTTCAAC+TGG + chr2.4:62327079-62327098 MS.gene02935:CDS 45.0%
GTGAAGGCTACCATTTGAGT+TGG - chr2.4:62326310-62326329 None:intergenic 45.0%
GTTAGGTGCTTACATGTTGG+AGG + chr2.4:62326653-62326672 MS.gene02935:CDS 45.0%
GTTGGAGGGATTAAGAGCAA+GGG + chr2.4:62326668-62326687 MS.gene02935:CDS 45.0%
TCAAATGGTAGCCTTCACCA+AGG + chr2.4:62326312-62326331 MS.gene02935:CDS 45.0%
TCCCAATCGTTTCATGCTAG+AGG + chr2.4:62326972-62326991 MS.gene02935:CDS 45.0%
TCTAACTGAATCAGTCACCG+AGG - chr2.4:62327522-62327541 None:intergenic 45.0%
TCTGTCTCAAAGACTGTTGC+TGG - chr2.4:62325918-62325937 None:intergenic 45.0%
TGTTGGAGGGATTAAGAGCA+AGG + chr2.4:62326667-62326686 MS.gene02935:CDS 45.0%
TTGGCGGTTCTCCAATTCAA+AGG + chr2.4:62326631-62326650 MS.gene02935:CDS 45.0%
! GGTGAAGATTTTGTCGCCTA+AGG + chr2.4:62327217-62327236 MS.gene02935:CDS 45.0%
!! TTGGGAAAGATGGTATCGGT+TGG + chr2.4:62326612-62326631 MS.gene02935:CDS 45.0%
!!! TAGAGACAGGCTTTTGAGAC+TGG + chr2.4:62327196-62327215 MS.gene02935:CDS 45.0%
ACCGAGGGACTCAATGGTAA+CGG - chr2.4:62327506-62327525 None:intergenic 50.0%
ACCGTTACCATTGAGTCCCT+CGG + chr2.4:62327502-62327521 MS.gene02935:CDS 50.0%
AGAGCAAGGGTGGAATCATC+AGG + chr2.4:62326681-62326700 MS.gene02935:CDS 50.0%
ATTCGGATGTACCTTGGTGG+AGG - chr2.4:62326470-62326489 None:intergenic 50.0%
CCTCCATTCGTTCGTGTAAC+AGG + chr2.4:62326939-62326958 MS.gene02935:CDS 50.0%
CCTGTTACACGAACGAATGG+AGG - chr2.4:62326942-62326961 None:intergenic 50.0%
GAGCAAGGGTGGAATCATCA+GGG + chr2.4:62326682-62326701 MS.gene02935:CDS 50.0%
GCTCTCAAGCATAAACCTGG+CGG + chr2.4:62326915-62326934 MS.gene02935:CDS 50.0%
GTGGAGGCTACCGTTTAAGT+TGG - chr2.4:62326454-62326473 None:intergenic 50.0%
GTTTACTCTAGTGCGGACGA+TGG + chr2.4:62326201-62326220 MS.gene02935:CDS 50.0%
TTAAACGGTAGCCTCCACCA+AGG + chr2.4:62326456-62326475 MS.gene02935:CDS 50.0%
! GTGGTAATGCATCCAACACC+AGG + chr2.4:62326112-62326131 MS.gene02935:CDS 50.0%
!! GGAAAGATGGTATCGGTTGG+CGG + chr2.4:62326615-62326634 MS.gene02935:CDS 50.0%
!! GGCTACCGTTTAAGTTGGAC+TGG - chr2.4:62326449-62326468 None:intergenic 50.0%
GGAGGGATTAAGAGCAAGGG+TGG + chr2.4:62326671-62326690 MS.gene02935:CDS 55.0%
TCAGTCACCGAGGGACTCAA+TGG - chr2.4:62327512-62327531 None:intergenic 55.0%
TCCATGCTCACGCTCTCGTC+CGG - chr2.4:62327170-62327189 None:intergenic 60.0%
CGAACGAATGGAGGTCCGCC+AGG - chr2.4:62326933-62326952 None:intergenic 65.0%
! ACCGGACGAGAGCGTGAGCA+TGG + chr2.4:62327166-62327185 MS.gene02935:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 62325865 62327655 62325865 ID=MS.gene02935
chr2.4 mRNA 62325865 62327655 62325865 ID=MS.gene02935.t1;Parent=MS.gene02935
chr2.4 exon 62325865 62327655 62325865 ID=MS.gene02935.t1.exon1;Parent=MS.gene02935.t1
chr2.4 CDS 62325865 62327655 62325865 ID=cds.MS.gene02935.t1;Parent=MS.gene02935.t1
Gene Sequence

>MS.gene02935

ATGTATACTTCTCAAAAGAAACGTAGTTCAGCCGGTATACCGTTATACTACCAGCAACAGTCTTTGAGACAGATTGATTCAAACAACAATCGTTTCAAAACGTTGCAAATCAATTCAAATAATGGTAGTATCAGTCAAAGAACCAATGTTTCTTTGGAAACAAGCAACTGGGAGAAGCAATTTTGTACCTTGGAATCTTCTCAAGTAGCCAATGAATTCATAGCTTTTGATTCTCCTTCATCTTCTAGTGGTAATGCATCCAACACCAGGAATCCTTTTTCACCACAATTTTCTCACTATGACTCCTCTGATAACACAAATGTATCGCCGTTAGGTGTTTACTCTAGTGCGGACGATGGTAGCTTTGAATTGAAGCACCAAATGAAAGAAATTGAGCTTTCATTGGTAGGTACCGATATAGAAGTATTCGGCAGCTGTCTGTCCAACTCAAATGGTAGCCTTCACCAAGGTACATCTCAAGATAATGAGTTTCAAATTGATGAATTAATGATTTCAGAGCTAGGCTATAAGGAATCGGAGATTTCATTGTTAGGTCCCAATTCAGATATCATCGACAGCTGCCAGTCCAACTTAAACGGTAGCCTCCACCAAGGTACATCCGAATATGATTGGTCTCAAATTGTAGAAATGATTCCAAAGCTAGACTTGAAAGAAGAACTTATTAGATGTGCACAGTTTGTATTTGATGGTGATTTTCAAAAAGCAATAGGATTCATGAATAACGTGTTGGGAAAGATGGTATCGGTTGGCGGTTCTCCAATTCAAAGGTTAGGTGCTTACATGTTGGAGGGATTAAGAGCAAGGGTGGAATCATCAGGGAGTGCAATTTATAATGCGTTGAAATGTGAAGAACCAACAAGCAATGAGTTGATGAGTGCTATGCACACTTTGTATCAGATTTGCCCATATTTTCAGTTTGCTTACATATCATCCAATGCTGTCATTTGTGAAGCAATGCAAAATGAATCAAGAATTCATATAATTGACTTCCAAATTGCACAAGGATCACAGTGGATGTTACTTTTGCATGCTCTCAAGCATAAACCTGGCGGACCTCCATTCGTTCGTGTAACAGGTATCGATGATTCCCAATCGTTTCATGCTAGAGGTGGAAGACTTGATATTGTAGGAAAAAAGCTAGAAGATTGTGCAAAAACTTGTAGAGTTCCATTTGAGTTTAATAGTGTTAAAATGTATGGATGTGAGGTTCAACTGGAAGATTTTGAAGTTCAACATGATGAAGTTCTTGCTGTGAATTTTCCTTTTGCTTTGCATCACATACCGGACGAGAGCGTGAGCATGGAGAATCATAGAGACAGGCTTTTGAGACTGGTGAAGATTTTGTCGCCTAAGGTTGTGTTATTTGTGGAGCAAGAGTCAAACACAAACACTTCTCCATTTTTACCAAGGTTTGCTGAGACTTTAAGTTATTACACTGCAATGTTTGAATCAATTGATGTGGCTCTTCCTAGAGATGATAAGAAGAGAATTAATGCAGAACAACACTGTGTGGCTCGCGATATCGTTAACATCATAGCTTGTGAGGGAGATGAGAGAGTTGAAAGACATGAACTGTTTGGAAAGTGGAGGAAAAGATTTTCAATGGCGGGTTTTGTACCGTTACCATTGAGTCCCTCGGTGACTGATTCAGTTAGAACTCTCTTGAAAGATTTCAATAAAGATTATAGGATTGAAGAAACAGATGTTGCTATCAATTTAGCGTGGAAGAGTAAAGTTATGTGCACATCTTCTGCTTGGAGATGTTATTGA
Protein sequence

>MS.gene02935.t1

MYTSQKKRSSAGIPLYYQQQSLRQIDSNNNRFKTLQINSNNGSISQRTNVSLETSNWEKQFCTLESSQVANEFIAFDSPSSSSGNASNTRNPFSPQFSHYDSSDNTNVSPLGVYSSADDGSFELKHQMKEIELSLVGTDIEVFGSCLSNSNGSLHQGTSQDNEFQIDELMISELGYKESEISLLGPNSDIIDSCQSNLNGSLHQGTSEYDWSQIVEMIPKLDLKEELIRCAQFVFDGDFQKAIGFMNNVLGKMVSVGGSPIQRLGAYMLEGLRARVESSGSAIYNALKCEEPTSNELMSAMHTLYQICPYFQFAYISSNAVICEAMQNESRIHIIDFQIAQGSQWMLLLHALKHKPGGPPFVRVTGIDDSQSFHARGGRLDIVGKKLEDCAKTCRVPFEFNSVKMYGCEVQLEDFEVQHDEVLAVNFPFALHHIPDESVSMENHRDRLLRLVKILSPKVVLFVEQESNTNTSPFLPRFAETLSYYTAMFESIDVALPRDDKKRINAEQHCVARDIVNIIACEGDERVERHELFGKWRKRFSMAGFVPLPLSPSVTDSVRTLLKDFNKDYRIEETDVAINLAWKSKVMCTSSAWRCY