AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene029403


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029403.t1 MTR_1g110840 95.238 231 11 0 1 231 1 231 1.48e-166 459
MS.gene029403.t1 MTR_6g453130 93.506 231 15 0 1 231 1 231 9.08e-164 451
MS.gene029403.t1 MTR_6g054080 90.043 231 23 0 1 231 1 231 8.37e-158 436
MS.gene029403.t1 MTR_6g054150 88.312 231 27 0 1 231 1 231 1.58e-155 431
MS.gene029403.t1 MTR_6g018270 55.172 174 57 7 1 168 1 159 6.81e-62 195
MS.gene029403.t1 MTR_7g007010 44.053 227 109 7 1 223 1 213 1.50e-55 179
MS.gene029403.t1 MTR_4g095600 56.303 119 48 2 3 119 1 117 2.19e-46 154
MS.gene029403.t1 MTR_5g015880 44.970 169 82 5 3 165 1 164 7.16e-45 150
MS.gene029403.t1 MTR_1g106420 44.882 127 63 2 3 124 1 125 9.37e-36 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029403.t1 AT1G68510 55.102 147 54 3 1 145 1 137 2.81e-53 171
MS.gene029403.t1 AT3G02550 40.529 227 122 5 1 225 1 216 3.82e-53 172
MS.gene029403.t1 AT1G67100 54.861 144 57 3 1 144 1 136 5.82e-50 163
MS.gene029403.t1 AT3G49940 53.659 123 55 1 3 125 1 121 1.54e-45 152
MS.gene029403.t1 AT5G67420 55.963 109 46 1 3 111 1 107 1.06e-43 147
MS.gene029403.t1 AT5G67420 55.963 109 46 1 3 111 1 107 1.06e-43 147
MS.gene029403.t1 AT4G37540 57.798 109 44 1 3 111 1 107 1.42e-43 147

Find 62 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCATGCCCTTGGGATTC+AGG 0.157225 1.4:-84516097 None:intergenic
ATGGGGCGCTTGGCTTATTT+TGG 0.165756 1.4:+84516565 MS.gene029403:CDS
AAGAATACCAACATGGAAAT+TGG 0.166066 1.4:+84516864 MS.gene029403:CDS
ACCGGTTCGGCCATAAAATT+TGG 0.172606 1.4:-84516131 None:intergenic
ATGGCGTATATCATAGGTTT+TGG 0.254539 1.4:-84516718 None:intergenic
TGCTGAGAATCAAGTGAATT+TGG 0.279066 1.4:+84516929 MS.gene029403:CDS
CCATGTTGGTATTCTTCAAA+AGG 0.367139 1.4:-84516857 None:intergenic
AGAATACCAACATGGAAATT+GGG 0.374565 1.4:+84516865 MS.gene029403:CDS
TGCCACATGGCGTATATCAT+AGG 0.386203 1.4:-84516724 None:intergenic
CTTTGATTGCCAATCAACTA+AGG 0.392207 1.4:+84516965 MS.gene029403:CDS
CATAAAATTTGGCAAGGAAG+AGG 0.404166 1.4:-84516120 None:intergenic
GACTGAACCAACTCGTGGCT+TGG 0.415502 1.4:-84516811 None:intergenic
ACTGAACCAACTCGTGGCTT+GGG 0.424285 1.4:-84516810 None:intergenic
GTGAATTTGGAACTCACTCT+TGG 0.424885 1.4:+84516942 MS.gene029403:CDS
GGAAGAGGGTGGCATGCCCT+TGG 0.443110 1.4:-84516105 None:intergenic
GCAACTGATCCACTCAAGGC+AGG 0.443950 1.4:-84516074 None:intergenic
TGGCATGCCCTTGGGATTCA+GGG 0.444879 1.4:-84516096 None:intergenic
AAAACTCAATTCAAAAGAGC+TGG 0.446487 1.4:+84516774 MS.gene029403:CDS
ATTGATGGGCAGACATCATC+AGG 0.455052 1.4:+84516666 MS.gene029403:CDS
GCAGTTGAAGCTGTGCTGAC+TGG 0.456964 1.4:+84516615 MS.gene029403:CDS
AGATTGGTGAATCCCACATA+TGG 0.458590 1.4:+84516546 MS.gene029403:CDS
TAAGCCAAGCGCCCCATATG+TGG 0.471835 1.4:-84516559 None:intergenic
CATGTTGGTATTCTTCAAAA+GGG 0.477135 1.4:-84516856 None:intergenic
GAAGAGGGTGGCATGCCCTT+GGG 0.479104 1.4:-84516104 None:intergenic
AATCATCACTGCAACCTTTG+CGG 0.482809 1.4:-84516039 None:intergenic
GGAGATTGAGCAAACCGGTT+CGG 0.494518 1.4:-84516144 None:intergenic
GATTGGTGAATCCCACATAT+GGG 0.508471 1.4:+84516547 MS.gene029403:CDS
GATATACGCCATGTGGCAAA+AGG 0.516720 1.4:+84516729 MS.gene029403:CDS
AATCCCACATATGGGGCGCT+TGG 0.518707 1.4:+84516555 MS.gene029403:CDS
GGTGAGGAGATTGAGCAAAC+CGG 0.524508 1.4:-84516149 None:intergenic
TCATCAAGCCCTGCCTTGAG+TGG 0.538225 1.4:+84516065 MS.gene029403:CDS
GTACCAATGTGGACATCAAA+GGG 0.546635 1.4:+84516751 MS.gene029403:CDS
TTTCCCTTTGATGTCCACAT+TGG 0.551024 1.4:-84516754 None:intergenic
GGTACCAATGTGGACATCAA+AGG 0.551224 1.4:+84516750 MS.gene029403:CDS
GTACGAGGCAGCTGGTAGAT+TGG 0.560594 1.4:+84516530 MS.gene029403:CDS
GAATACCAACATGGAAATTG+GGG 0.561319 1.4:+84516866 MS.gene029403:CDS
CTGAAGCCCAAGCCACGAGT+TGG 0.576033 1.4:+84516804 MS.gene029403:CDS
AGTCTCCCCAATTTCCATGT+TGG 0.576332 1.4:-84516871 None:intergenic
ATAAAATTTGGCAAGGAAGA+GGG 0.578936 1.4:-84516119 None:intergenic
TTCGGCCATAAAATTTGGCA+AGG 0.579687 1.4:-84516126 None:intergenic
TAATGAAGTTGAGTTGCAAT+GGG 0.583194 1.4:+84516005 None:intergenic
TTTGATTGCCAATCAACTAA+GGG 0.586746 1.4:+84516966 MS.gene029403:CDS
AAGCCAAGCGCCCCATATGT+GGG 0.589682 1.4:-84516558 None:intergenic
AAACTCAATTCAAAAGAGCT+GGG 0.590181 1.4:+84516775 MS.gene029403:CDS
CAACTGATCCACTCAAGGCA+GGG 0.595554 1.4:-84516073 None:intergenic
ATCAGTTGCCCTGAATCCCA+AGG 0.599742 1.4:+84516088 MS.gene029403:CDS
ATAATGAAGTTGAGTTGCAA+TGG 0.608858 1.4:+84516004 None:intergenic
TCATTGTTGTACGAGGCAGC+TGG 0.614180 1.4:+84516522 MS.gene029403:CDS
ACGAGTTGGTTCAGTCGACT+CGG 0.622355 1.4:+84516818 MS.gene029403:CDS
CAGGGCAACTGATCCACTCA+AGG 0.625744 1.4:-84516078 None:intergenic
GGGTGTCGTGTGCTCCGCAA+AGG 0.627575 1.4:+84516025 MS.gene029403:CDS
ATTGGTGAATCCCACATATG+GGG 0.640018 1.4:+84516548 MS.gene029403:CDS
TTTGATTGGTAGCATTGGTG+AGG 0.643249 1.4:-84516165 None:intergenic
AAATTGGGGAGACTTCATCA+AGG 0.644743 1.4:+84516880 MS.gene029403:CDS
AATTGGGGAGACTTCATCAA+GGG 0.644822 1.4:+84516881 MS.gene029403:CDS
AATCAAAATCATAATCCACC+AGG 0.664735 1.4:+84516181 MS.gene029403:CDS
TCAGTTGCCCTGAATCCCAA+GGG 0.669183 1.4:+84516089 MS.gene029403:CDS
GAGTCGACTGAACCAACTCG+TGG 0.679210 1.4:-84516816 None:intergenic
AACCTATGATATACGCCATG+TGG 0.679816 1.4:+84516722 MS.gene029403:CDS
AAATTTGGCAAGGAAGAGGG+TGG 0.680062 1.4:-84516116 None:intergenic
GTTTAAATCATTGTTGTACG+AGG 0.684324 1.4:+84516515 MS.gene029403:CDS
TGTGGCAAAAGGTACCAATG+TGG 0.731996 1.4:+84516740 MS.gene029403:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATCTAACACTTAAATGA+TGG + chr1.4:84516225-84516244 MS.gene029403:intron 20.0%
!!! GAATCTATGAAAAGATTTTA+TGG - chr1.4:84516423-84516442 None:intergenic 20.0%
!!! GTTTAATTTATGATCCATAA+CGG - chr1.4:84516382-84516401 None:intergenic 20.0%
!! ACAGTTTTACAATGATTGAT+GGG + chr1.4:84516652-84516671 MS.gene029403:CDS 25.0%
AAAACTCAATTCAAAAGAGC+TGG + chr1.4:84516774-84516793 MS.gene029403:CDS 30.0%
AAACTCAATTCAAAAGAGCT+GGG + chr1.4:84516775-84516794 MS.gene029403:CDS 30.0%
AAGAATACCAACATGGAAAT+TGG + chr1.4:84516864-84516883 MS.gene029403:CDS 30.0%
AATCAAAATCATAATCCACC+AGG + chr1.4:84516181-84516200 MS.gene029403:CDS 30.0%
AGAATACCAACATGGAAATT+GGG + chr1.4:84516865-84516884 MS.gene029403:CDS 30.0%
ATAAAATTTGGCAAGGAAGA+GGG - chr1.4:84516122-84516141 None:intergenic 30.0%
ATGGAACATAAATGAGACAT+AGG - chr1.4:84516404-84516423 None:intergenic 30.0%
CATGTTGGTATTCTTCAAAA+GGG - chr1.4:84516859-84516878 None:intergenic 30.0%
GTTTAAATCATTGTTGTACG+AGG + chr1.4:84516515-84516534 MS.gene029403:CDS 30.0%
TAAAAAAGTTTGTCGTGTAG+CGG - chr1.4:84516314-84516333 None:intergenic 30.0%
TTTGATTGCCAATCAACTAA+GGG + chr1.4:84516966-84516985 MS.gene029403:CDS 30.0%
! ACCACAATTTAAAACCATGA+TGG - chr1.4:84516277-84516296 None:intergenic 30.0%
! CAATTTAAAACCATGATGGA+TGG - chr1.4:84516273-84516292 None:intergenic 30.0%
! GACAGTTTTACAATGATTGA+TGG + chr1.4:84516651-84516670 MS.gene029403:CDS 30.0%
! TCTAACACTTAAATGATGGA+TGG + chr1.4:84516229-84516248 MS.gene029403:intron 30.0%
!!! ATGATTTTGATTGGTAGCAT+TGG - chr1.4:84516173-84516192 None:intergenic 30.0%
!!! TCCATCATGGTTTTAAATTG+TGG + chr1.4:84516273-84516292 MS.gene029403:intron 30.0%
!!! TGGTGGATTATGATTTTGAT+TGG - chr1.4:84516182-84516201 None:intergenic 30.0%
ATCAATGATTCCATCCATCA+TGG + chr1.4:84516260-84516279 MS.gene029403:intron 35.0%
CATAAAATTTGGCAAGGAAG+AGG - chr1.4:84516123-84516142 None:intergenic 35.0%
CCATGTTGGTATTCTTCAAA+AGG - chr1.4:84516860-84516879 None:intergenic 35.0%
CTTTGATTGCCAATCAACTA+AGG + chr1.4:84516965-84516984 MS.gene029403:CDS 35.0%
GAATACCAACATGGAAATTG+GGG + chr1.4:84516866-84516885 MS.gene029403:CDS 35.0%
TATGATCCATAACGGTATTG+CGG - chr1.4:84516374-84516393 None:intergenic 35.0%
TGCTGAGAATCAAGTGAATT+TGG + chr1.4:84516929-84516948 MS.gene029403:CDS 35.0%
! ATGGCGTATATCATAGGTTT+TGG - chr1.4:84516721-84516740 None:intergenic 35.0%
! CTCTTCCTTGCCAAATTTTA+TGG + chr1.4:84516121-84516140 MS.gene029403:CDS 35.0%
! TGGCGTATATCATAGGTTTT+GGG - chr1.4:84516720-84516739 None:intergenic 35.0%
! TTTTCTTGCCCTTAGTTGAT+TGG - chr1.4:84516977-84516996 None:intergenic 35.0%
!! CCTTTTGAAGAATACCAACA+TGG + chr1.4:84516857-84516876 MS.gene029403:CDS 35.0%
AAATTGGGGAGACTTCATCA+AGG + chr1.4:84516880-84516899 MS.gene029403:CDS 40.0%
AACCTATGATATACGCCATG+TGG + chr1.4:84516722-84516741 MS.gene029403:CDS 40.0%
AATCATCACTGCAACCTTTG+CGG - chr1.4:84516042-84516061 None:intergenic 40.0%
AATTGGGGAGACTTCATCAA+GGG + chr1.4:84516881-84516900 MS.gene029403:CDS 40.0%
AGATTGGTGAATCCCACATA+TGG + chr1.4:84516546-84516565 MS.gene029403:CDS 40.0%
ATTGGTGAATCCCACATATG+GGG + chr1.4:84516548-84516567 MS.gene029403:CDS 40.0%
GATTGGTGAATCCCACATAT+GGG + chr1.4:84516547-84516566 MS.gene029403:CDS 40.0%
GTACCAATGTGGACATCAAA+GGG + chr1.4:84516751-84516770 MS.gene029403:CDS 40.0%
TTCGGCCATAAAATTTGGCA+AGG - chr1.4:84516129-84516148 None:intergenic 40.0%
TTTCCCTTTGATGTCCACAT+TGG - chr1.4:84516757-84516776 None:intergenic 40.0%
! GGCGTATATCATAGGTTTTG+GGG - chr1.4:84516719-84516738 None:intergenic 40.0%
! GTGAATTTGGAACTCACTCT+TGG + chr1.4:84516942-84516961 MS.gene029403:CDS 40.0%
!! GTGACCAACTTAAGTTGATG+CGG + chr1.4:84516348-84516367 MS.gene029403:intron 40.0%
!! TTTGATTGGTAGCATTGGTG+AGG - chr1.4:84516168-84516187 None:intergenic 40.0%
!!! GCTTATTTTGGACAGCGAAT+TGG + chr1.4:84516577-84516596 MS.gene029403:CDS 40.0%
AAATTTGGCAAGGAAGAGGG+TGG - chr1.4:84516119-84516138 None:intergenic 45.0%
AGTCTCCCCAATTTCCATGT+TGG - chr1.4:84516874-84516893 None:intergenic 45.0%
AGTGAACATAGCTAGCTACC+TGG - chr1.4:84516202-84516221 None:intergenic 45.0%
GATATACGCCATGTGGCAAA+AGG + chr1.4:84516729-84516748 MS.gene029403:CDS 45.0%
GGTACCAATGTGGACATCAA+AGG + chr1.4:84516750-84516769 MS.gene029403:CDS 45.0%
TGCCACATGGCGTATATCAT+AGG - chr1.4:84516727-84516746 None:intergenic 45.0%
! ATTGATGGGCAGACATCATC+AGG + chr1.4:84516666-84516685 MS.gene029403:CDS 45.0%
! CATTGGTACCTTTTGCCACA+TGG - chr1.4:84516740-84516759 None:intergenic 45.0%
! GCCAAATTTTATGGCCGAAC+CGG + chr1.4:84516130-84516149 MS.gene029403:CDS 45.0%
!! ACCGGTTCGGCCATAAAATT+TGG - chr1.4:84516134-84516153 None:intergenic 45.0%
!! TGTGGCAAAAGGTACCAATG+TGG + chr1.4:84516740-84516759 MS.gene029403:CDS 45.0%
ACTGAACCAACTCGTGGCTT+GGG - chr1.4:84516813-84516832 None:intergenic 50.0%
ATCAGTTGCCCTGAATCCCA+AGG + chr1.4:84516088-84516107 MS.gene029403:CDS 50.0%
CAACTGATCCACTCAAGGCA+GGG - chr1.4:84516076-84516095 None:intergenic 50.0%
GAACATAGCTAGCTACCTGG+TGG - chr1.4:84516199-84516218 None:intergenic 50.0%
GGAGATTGAGCAAACCGGTT+CGG - chr1.4:84516147-84516166 None:intergenic 50.0%
GGTGAGGAGATTGAGCAAAC+CGG - chr1.4:84516152-84516171 None:intergenic 50.0%
TCAGTTGCCCTGAATCCCAA+GGG + chr1.4:84516089-84516108 MS.gene029403:CDS 50.0%
TCATTGTTGTACGAGGCAGC+TGG + chr1.4:84516522-84516541 MS.gene029403:CDS 50.0%
!! ACGAGTTGGTTCAGTCGACT+CGG + chr1.4:84516818-84516837 MS.gene029403:CDS 50.0%
!! ATGGGGCGCTTGGCTTATTT+TGG + chr1.4:84516565-84516584 MS.gene029403:CDS 50.0%
AAGCCAAGCGCCCCATATGT+GGG - chr1.4:84516561-84516580 None:intergenic 55.0%
ATGCGGCCGCAATACCGTTA+TGG + chr1.4:84516365-84516384 MS.gene029403:intron 55.0%
CAGGGCAACTGATCCACTCA+AGG - chr1.4:84516081-84516100 None:intergenic 55.0%
GACTGAACCAACTCGTGGCT+TGG - chr1.4:84516814-84516833 None:intergenic 55.0%
GAGTCGACTGAACCAACTCG+TGG - chr1.4:84516819-84516838 None:intergenic 55.0%
GCAACTGATCCACTCAAGGC+AGG - chr1.4:84516077-84516096 None:intergenic 55.0%
GCGGCCGCATCAACTTAAGT+TGG - chr1.4:84516355-84516374 None:intergenic 55.0%
GTACGAGGCAGCTGGTAGAT+TGG + chr1.4:84516530-84516549 MS.gene029403:CDS 55.0%
TAAGCCAAGCGCCCCATATG+TGG - chr1.4:84516562-84516581 None:intergenic 55.0%
TCATCAAGCCCTGCCTTGAG+TGG + chr1.4:84516065-84516084 MS.gene029403:CDS 55.0%
TGGCATGCCCTTGGGATTCA+GGG - chr1.4:84516099-84516118 None:intergenic 55.0%
! AATCCCACATATGGGGCGCT+TGG + chr1.4:84516555-84516574 MS.gene029403:CDS 55.0%
! GCAGTTGAAGCTGTGCTGAC+TGG + chr1.4:84516615-84516634 MS.gene029403:CDS 55.0%
CTGAAGCCCAAGCCACGAGT+TGG + chr1.4:84516804-84516823 MS.gene029403:CDS 60.0%
GAAGAGGGTGGCATGCCCTT+GGG - chr1.4:84516107-84516126 None:intergenic 60.0%
GTGGCATGCCCTTGGGATTC+AGG - chr1.4:84516100-84516119 None:intergenic 60.0%
GGAAGAGGGTGGCATGCCCT+TGG - chr1.4:84516108-84516127 None:intergenic 65.0%
! GGGTGTCGTGTGCTCCGCAA+AGG + chr1.4:84516025-84516044 MS.gene029403:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 84516007 84517010 84516007 ID=MS.gene029403
chr1.4 mRNA 84516007 84517010 84516007 ID=MS.gene029403.t1;Parent=MS.gene029403
chr1.4 exon 84516007 84516202 84516007 ID=MS.gene029403.t1.exon1;Parent=MS.gene029403.t1
chr1.4 CDS 84516007 84516202 84516007 ID=cds.MS.gene029403.t1;Parent=MS.gene029403.t1
chr1.4 exon 84516511 84517010 84516511 ID=MS.gene029403.t1.exon2;Parent=MS.gene029403.t1
chr1.4 CDS 84516511 84517010 84516511 ID=cds.MS.gene029403.t1;Parent=MS.gene029403.t1
Gene Sequence

>MS.gene029403

ATGAAGTTGAGTTGCAATGGGTGTCGTGTGCTCCGCAAAGGTTGCAGTGATGATTGTATCATCAAGCCCTGCCTTGAGTGGATCAGTTGCCCTGAATCCCAAGGGCATGCCACCCTCTTCCTTGCCAAATTTTATGGCCGAACCGGTTTGCTCAATCTCCTCACCAATGCTACCAATCAAAATCATAATCCACCAGGTAGCTAGCTATGTTCACTATCATAATCTAACACTTAAATGATGGATGGAATGATTGATCAATGATTCCATCCATCATGGTTTTAAATTGTGGTTGTTGTTGCAGTTGCCGCTACACGACAAACTTTTTTAATGTAATAATAAATGTGACCAACTTAAGTTGATGCGGCCGCAATACCGTTATGGATCATAAATTAAACCTATGTCTCATTTATGTTCCATAAAATCTTTTCATAGATTCTACATCAAATTTGATTTTATTTTTTAATATATGATGATTAATGTCTTTAATTTTGTCACAATTTTCAGCTGTGTTTAAATCATTGTTGTACGAGGCAGCTGGTAGATTGGTGAATCCCACATATGGGGCGCTTGGCTTATTTTGGACAGCGAATTGGAGTCGTTGTGAAGCTGCAGTTGAAGCTGTGCTGACTGGATCGAATATTAATGACAGTTTTACAATGATTGATGGGCAGACATCATCAGGTACACTCAATGCTGAAAATCATGTTCTCCCCAAAACCTATGATATACGCCATGTGGCAAAAGGTACCAATGTGGACATCAAAGGGAAAACTCAATTCAAAAGAGCTGGGCAAGTTCTGAAGCCCAAGCCACGAGTTGGTTCAGTCGACTCGGCTACATTGTTGAAATCCCTTTTGAAGAATACCAACATGGAAATTGGGGAGACTTCATCAAGGGTTCAAACAGAGAAAATTAATGAAGCTGCTGAGAATCAAGTGAATTTGGAACTCACTCTTGGCTTTGATTGCCAATCAACTAAGGGCAAGAAAATATTAGATAAATGA

Protein sequence

>MS.gene029403.t1

MKLSCNGCRVLRKGCSDDCIIKPCLEWISCPESQGHATLFLAKFYGRTGLLNLLTNATNQNHNPPAVFKSLLYEAAGRLVNPTYGALGLFWTANWSRCEAAVEAVLTGSNINDSFTMIDGQTSSGTLNAENHVLPKTYDIRHVAKGTNVDIKGKTQFKRAGQVLKPKPRVGSVDSATLLKSLLKNTNMEIGETSSRVQTEKINEAAENQVNLELTLGFDCQSTKGKKILDK