AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene029485

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029485.t1 MTR_1g109320 98.599 357 3 1 1 355 1 357 0.0 731
MS.gene029485.t1 MTR_5g090040 81.707 82 6 1 262 343 78 150 2.43e-43 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029485.t1 AT1G68200 48.684 304 111 8 62 350 29 302 3.24e-84 258
MS.gene029485.t1 AT1G68200 48.684 304 111 8 62 350 29 302 3.24e-84 258
MS.gene029485.t1 AT1G68200 48.805 293 126 6 62 350 29 301 1.09e-82 254
MS.gene029485.t1 AT1G68200 48.805 293 126 6 62 350 29 301 1.09e-82 254
MS.gene029485.t1 AT1G66810 61.111 144 49 1 200 343 168 304 5.21e-55 182
MS.gene029485.t1 AT1G66810 61.111 144 49 1 200 343 168 304 5.21e-55 182
MS.gene029485.t1 AT1G68200 41.026 156 72 5 62 217 29 164 4.70e-22 92.8
MS.gene029485.t1 AT1G68200 41.026 156 72 5 62 217 29 164 4.70e-22 92.8

Find 106 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTGCAGGATGGTCCTTGC+TGG 0.159266 1.2:-81080962 MS.gene029485:CDS
CTGCAGAGCTTCAACTTCTT+TGG 0.179049 1.2:+81083489 None:intergenic
TCTTTCCAGCTTCATTGATC+TGG 0.216499 1.2:+81080859 None:intergenic
GCCGCCGTTGCTGCTGTTTC+CGG 0.266911 1.2:+81083630 None:intergenic
CAACTTCTTTGGAACTTTCC+AGG 0.279329 1.2:+81083500 None:intergenic
TAGCGTGGGTGGCGGATCAC+TGG 0.295264 1.2:+81080995 None:intergenic
GATCTAACAGAAATGCTCTT+TGG 0.302932 1.2:+81083160 None:intergenic
GCCGATGCCGTAACCGCTTT+TGG 0.311876 1.2:+81083741 None:intergenic
GTCAATTCGCTCATGGCATT+GGG 0.349776 1.2:-81081027 MS.gene029485:CDS
GGTTTCCGTGGCTTGAATCT+TGG 0.355570 1.2:-81083325 MS.gene029485:CDS
CTTTCCAGCTTCATTGATCT+GGG 0.356760 1.2:+81080860 None:intergenic
CTTTATCCTTACTCTGACCT+TGG 0.377357 1.2:-81083598 MS.gene029485:CDS
GCGGGAGCGAGTAGCGCCTT+TGG 0.377546 1.2:+81083075 None:intergenic
CATGGCGGAAATGGCATCTA+TGG 0.379484 1.2:+81080921 None:intergenic
GAAACTGGCACATGCCCTTA+TGG 0.383739 1.2:-81081058 MS.gene029485:CDS
TAATAATAACAAGGAGTTGC+AGG 0.387088 1.2:-81083257 MS.gene029485:CDS
CCGTCATCCAAAAGCGGTTA+CGG 0.390482 1.2:-81083748 MS.gene029485:CDS
GATACAGAAGCATCAGGATA+TGG 0.396830 1.2:-81083554 MS.gene029485:CDS
CTGTTTCCGGCGAGCGATGA+TGG 0.396990 1.2:+81083643 None:intergenic
TGTAACAAATGGCAGGAAAC+TGG 0.399409 1.2:-81081073 MS.gene029485:CDS
TCTGCAGGATGGTCCTTGCT+GGG 0.402584 1.2:-81080961 MS.gene029485:CDS
CTTCTGCAACTCTTTGTTCA+CGG 0.410248 1.2:+81083444 None:intergenic
CCTTCTCGTTCAAGCTTCGC+TGG 0.421155 1.2:-81083413 MS.gene029485:CDS
CTGTAAGTGCATGGCGGAAA+TGG 0.422540 1.2:+81080912 None:intergenic
TATGTTTCTCAGTCGTCTCC+TGG 0.438714 1.2:-81083518 MS.gene029485:CDS
CAAACGGAGTTGATTGAGTC+TGG 0.444450 1.2:+81083359 None:intergenic
TGTCAATTCGCTCATGGCAT+TGG 0.444853 1.2:-81081028 MS.gene029485:CDS
CTTGAAGCTTGCTCCGCCTC+CGG 0.468503 1.2:-81083128 MS.gene029485:CDS
AATTGACAGTGGTCTCCATA+AGG 0.472418 1.2:+81081043 None:intergenic
CGTGGCTTGAATCTTGGAGA+TGG 0.475661 1.2:-81083319 MS.gene029485:CDS
GAAAGGAAAACTGTGCTGAT+TGG 0.476246 1.2:-81083297 MS.gene029485:CDS
TCCAAAAGCGGTTACGGCAT+CGG 0.480005 1.2:-81083742 MS.gene029485:CDS
AGCATTTCTGTTAGATCCAA+TGG 0.486635 1.2:-81083154 MS.gene029485:CDS
CGTCGGACTCTCATCAGATT+CGG 0.492348 1.2:+81083228 None:intergenic
GAAGCTCTGCAGCAGGAAAA+CGG 0.500754 1.2:-81083478 MS.gene029485:CDS
TCTCTTGTTCTGTAAGTGCA+TGG 0.501589 1.2:+81080903 None:intergenic
GGCGTTCGACTGCATGTGAC+CGG 0.502190 1.2:+81083708 None:intergenic
AAACCTTGCAACACATCAAA+CGG 0.507246 1.2:+81083343 None:intergenic
TGAGAATAACGGTGTTGAAA+CGG 0.507485 1.2:-81083197 MS.gene029485:CDS
AGCGTGGGTGGCGGATCACT+GGG 0.510898 1.2:+81080996 None:intergenic
GCTGGGGTTGTGTGCCCGTA+TGG 0.512898 1.2:-81080944 MS.gene029485:CDS
ATGCGTGATACAGAAGCATC+AGG 0.518868 1.2:-81083560 MS.gene029485:CDS
TCGCTGGAGAACCGATTCAA+TGG 0.519751 1.2:-81083397 MS.gene029485:CDS
AAATGGCATCTATGGCCATA+CGG 0.521463 1.2:+81080929 None:intergenic
CTCGCCGGAAACAGCAGCAA+CGG 0.524709 1.2:-81083634 MS.gene029485:CDS
GGAAACAGCAGCAACGGCGG+CGG 0.524773 1.2:-81083628 MS.gene029485:CDS
GCCGGAAACAGCAGCAACGG+CGG 0.525054 1.2:-81083631 MS.gene029485:CDS
GCTACAAAACTGAGGTCTGC+AGG 0.527724 1.2:-81080976 MS.gene029485:CDS
TTAGTTACGACGGCCGGAGG+CGG 0.529646 1.2:+81083115 None:intergenic
AGCGAGTAGCGCCTTTGGTG+CGG 0.529924 1.2:+81083080 None:intergenic
AGAAGTTGAAGCTCTGCAGC+AGG 0.529958 1.2:-81083485 MS.gene029485:CDS
ATTGACAGTGGTCTCCATAA+GGG 0.530052 1.2:+81081044 None:intergenic
TAAGCCCAGATCAATGAAGC+TGG 0.530814 1.2:-81080864 MS.gene029485:CDS
AGCAAGCTTCAAGTAGCCAT+TGG 0.531440 1.2:+81083138 None:intergenic
AGACTGAGCTCTGTAACAAA+TGG 0.531908 1.2:-81081084 MS.gene029485:CDS
AATGGCATCTATGGCCATAC+GGG 0.531942 1.2:+81080930 None:intergenic
AGGATCCGCCACCATTGAAT+CGG 0.534519 1.2:+81083386 None:intergenic
GCTCAGTCTTGAACATCCCC+TGG 0.541107 1.2:+81081098 None:intergenic
AACAATGCTTGCCGCACCAA+AGG 0.543012 1.2:-81083091 MS.gene029485:CDS
TCTGTTCCAAGGTCAGAGTA+AGG 0.546191 1.2:+81083592 None:intergenic
TGTATCAGTGGAGGAAGCGC+GGG 0.553790 1.2:+81083057 None:intergenic
GATCTTCCATCATCGCTCGC+CGG 0.559218 1.2:-81083649 MS.gene029485:CDS
ACTCCGTTTGATGTGTTGCA+AGG 0.560653 1.2:-81083346 MS.gene029485:CDS
TGAGCTCTGTAACAAATGGC+AGG 0.566245 1.2:-81081080 MS.gene029485:CDS
AGCATTGTTGTTAGTTACGA+CGG 0.567553 1.2:+81083105 None:intergenic
CATCTCCAAGATTCAAGCCA+CGG 0.567612 1.2:+81083320 None:intergenic
TGTGCGAGGAGGGCAGAAAG+AGG 0.570699 1.2:-81081149 MS.gene029485:CDS
GACCACTGTCAATTCGCTCA+TGG 0.571725 1.2:-81081034 MS.gene029485:CDS
CTGCAGGATGGTCCTTGCTG+GGG 0.572424 1.2:-81080960 MS.gene029485:CDS
CTGTATCAGTGGAGGAAGCG+CGG 0.573224 1.2:+81083056 None:intergenic
AGAGGAAGAGCCTCTTGAGA+TGG 0.573431 1.2:-81081131 MS.gene029485:CDS
AAAAGGTATTTGTGCGAGGA+GGG 0.574881 1.2:-81081159 MS.gene029485:CDS
GAGAACCGATTCAATGGTGG+CGG 0.578241 1.2:-81083391 MS.gene029485:CDS
CCAGCGAAGCTTGAACGAGA+AGG 0.578885 1.2:+81083413 None:intergenic
TGAGATGGTTGTGTACAACC+AGG 0.582022 1.2:-81081116 MS.gene029485:CDS
CTTGAATCTTGGAGATGGAA+AGG 0.582921 1.2:-81083314 MS.gene029485:CDS
CGGAGTTGATTGAGTCTGGA+CGG 0.589982 1.2:+81083363 None:intergenic
GCAACTCTTTGTTCACGGCA+CGG 0.595404 1.2:+81083449 None:intergenic
GGTCACATGCAGTCGAACGC+CGG 0.598115 1.2:-81083706 MS.gene029485:CDS
CAAAAGGTATTTGTGCGAGG+AGG 0.603500 1.2:-81081160 MS.gene029485:CDS
TTGTTGTTAGTTACGACGGC+CGG 0.605294 1.2:+81083109 None:intergenic
GATGTGTTGCAAGGTTTCCG+TGG 0.607851 1.2:-81083337 MS.gene029485:CDS
CAAAACTGAGGTCTGCAGGA+TGG 0.616724 1.2:-81080972 MS.gene029485:CDS
ACGAGTGTGATTGAGAATAA+CGG 0.622937 1.2:-81083208 MS.gene029485:CDS
ATGAGTCGTACTCTGTTCCA+AGG 0.623681 1.2:+81083581 None:intergenic
ACGGGCACACAACCCCAGCA+AGG 0.626804 1.2:+81080948 None:intergenic
CAGCAAAAGGTATTTGTGCG+AGG 0.628338 1.2:-81081163 MS.gene029485:CDS
CTGGAGAACCGATTCAATGG+TGG 0.634958 1.2:-81083394 MS.gene029485:CDS
TGTTCTCCGTCATCCAAAAG+CGG 0.637659 1.2:-81083754 MS.gene029485:CDS
GAGATGGTTGTGTACAACCA+GGG 0.638365 1.2:-81081115 MS.gene029485:CDS
TTGTTAGTTACGACGGCCGG+AGG 0.641381 1.2:+81083112 None:intergenic
TCAATTCGCTCATGGCATTG+GGG 0.646224 1.2:-81081026 MS.gene029485:CDS
TTGTACACAACCATCTCAAG+AGG 0.647753 1.2:+81081121 None:intergenic
GGCATCGGCGCAAACAACGC+CGG 0.650373 1.2:-81083727 MS.gene029485:CDS
ACCGATCAAAATGCATAACA+AGG 0.654571 1.2:-81083782 None:intergenic
TGTGCTTACAACTGTATCAG+TGG 0.668219 1.2:+81083045 None:intergenic
TAACATCAATAATAATAACA+AGG 0.675567 1.2:-81083266 MS.gene029485:CDS
TGCCATGAGCGAATTGACAG+TGG 0.682669 1.2:+81081032 None:intergenic
CTTGTTCTGTAAGTGCATGG+CGG 0.684544 1.2:+81080906 None:intergenic
TTATTCTCAATCACACTCGT+CGG 0.688787 1.2:+81083211 None:intergenic
AGTTGATTGAGTCTGGACGG+AGG 0.689033 1.2:+81083366 None:intergenic
GTACAACGAAGCAAACAAGC+CGG 0.690951 1.2:+81083687 None:intergenic
GCTTACAACTGTATCAGTGG+AGG 0.693535 1.2:+81083048 None:intergenic
ATAACGGTGTTGAAACGGAG+AGG 0.717587 1.2:-81083192 MS.gene029485:CDS
CCACCCACGCTACAAAACTG+AGG 0.721664 1.2:-81080984 MS.gene029485:CDS
AGATGGTTGTGTACAACCAG+GGG 0.741581 1.2:-81081114 MS.gene029485:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTTATGTTGAAAATA+AGG - chr1.2:81083124-81083143 MS.gene029485:CDS 10.0%
!! AAAGCATATATTAATAATAG+AGG + chr1.2:81083103-81083122 None:intergenic 15.0%
!! ATTATAATGTAAAAACTGTA+TGG + chr1.2:81082582-81082601 None:intergenic 15.0%
!! TAACAAAACAAATAACTAAA+TGG + chr1.2:81083008-81083027 None:intergenic 15.0%
!! TAACATCAATAATAATAACA+AGG - chr1.2:81081362-81081381 MS.gene029485:intron 15.0%
!!! CAGTTTTTACATTATAATTA+AGG - chr1.2:81082584-81082603 MS.gene029485:intron 15.0%
!!! TTTTTACATTATAATTAAGG+TGG - chr1.2:81082587-81082606 MS.gene029485:intron 15.0%
!! TAGATTTGATTGTTGATATT+CGG - chr1.2:81083403-81083422 MS.gene029485:CDS 20.0%
!!! AATTGTGTTCAATTTTGAAA+TGG - chr1.2:81083182-81083201 MS.gene029485:CDS 20.0%
!!! CGATATTTTAAACTAATAGA+TGG - chr1.2:81082321-81082340 MS.gene029485:intron 20.0%
!!! GCATCAAAATTTAACTTAAT+TGG + chr1.2:81082557-81082576 None:intergenic 20.0%
!!! TGGTTTTTCTAAGTTTTAAA+GGG - chr1.2:81082841-81082860 MS.gene029485:intron 20.0%
!!! TTGGTTTTTCTAAGTTTTAA+AGG - chr1.2:81082840-81082859 MS.gene029485:intron 20.0%
!!! TTTAAAACTTAGAAAAACCA+AGG + chr1.2:81082841-81082860 None:intergenic 20.0%
! TCATACAATGTCTACATTAT+AGG - chr1.2:81082688-81082707 MS.gene029485:intron 25.0%
! TTACATTATTATGAACGTCA+AGG + chr1.2:81083333-81083352 None:intergenic 25.0%
!!! AAAAACCAAGGTATTGATTT+TGG + chr1.2:81082829-81082848 None:intergenic 25.0%
!!! ACATTATAGGAGTATTTAAG+AGG - chr1.2:81082701-81082720 MS.gene029485:intron 25.0%
!!! ATGAGATAAAGATTTTAGAG+AGG - chr1.2:81082349-81082368 MS.gene029485:intron 25.0%
!!! GGTTTTTCTAAGTTTTAAAG+GGG - chr1.2:81082842-81082861 MS.gene029485:intron 25.0%
!!! TAAGTTTTAAAGGGGATAAA+TGG - chr1.2:81082850-81082869 MS.gene029485:intron 25.0%
AAACATTAGTATGACTGTCA+AGG + chr1.2:81083237-81083256 None:intergenic 30.0%
AACCAACAACTAGTATAAAC+TGG + chr1.2:81081968-81081987 None:intergenic 30.0%
ACATTTATGATAGCCATGTA+TGG - chr1.2:81082763-81082782 MS.gene029485:intron 30.0%
ACTAACAAGCAAGTCAAATT+TGG + chr1.2:81081814-81081833 None:intergenic 30.0%
CAGGTTAAAATGTCACAAAA+AGG + chr1.2:81082059-81082078 None:intergenic 30.0%
CTGTATATGGTTTCAAATTG+CGG - chr1.2:81082098-81082117 MS.gene029485:intron 30.0%
GAAAAAACAACCAAACTACA+AGG + chr1.2:81081908-81081927 None:intergenic 30.0%
GTAATGTCTTGAATCTTCAT+TGG - chr1.2:81083044-81083063 MS.gene029485:intron 30.0%
TAATAATAACAAGGAGTTGC+AGG - chr1.2:81081371-81081390 MS.gene029485:intron 30.0%
TACTTCGAGATAAAGATGAA+TGG - chr1.2:81083151-81083170 MS.gene029485:CDS 30.0%
TATATAGAACTAGCAAGACT+AGG - chr1.2:81082653-81082672 MS.gene029485:intron 30.0%
! AAGCAAGGTTTTACTTCATT+TGG + chr1.2:81081854-81081873 None:intergenic 30.0%
! AAGGTTTTACTTCATTTGGA+AGG + chr1.2:81081850-81081869 None:intergenic 30.0%
! GTCCACTCATTGTTATTAAT+CGG + chr1.2:81083377-81083396 None:intergenic 30.0%
! TGTCACTCAATTTCTTTTCA+TGG - chr1.2:81082919-81082938 MS.gene029485:intron 30.0%
!! TTTTAAAGGGGATAAATGGT+TGG - chr1.2:81082854-81082873 MS.gene029485:intron 30.0%
!!! ACAACCATGGTTTTAAAATG+CGG + chr1.2:81082515-81082534 None:intergenic 30.0%
AAACCATATACAGAGCACAT+TGG + chr1.2:81082091-81082110 None:intergenic 35.0%
AAACCTTGCAACACATCAAA+CGG + chr1.2:81081288-81081307 None:intergenic 35.0%
ACGAGTGTGATTGAGAATAA+CGG - chr1.2:81081420-81081439 MS.gene029485:intron 35.0%
AGCATTTCTGTTAGATCCAA+TGG - chr1.2:81081474-81081493 MS.gene029485:intron 35.0%
ATGGCAAACTAGAGCTAATT+TGG + chr1.2:81082006-81082025 None:intergenic 35.0%
CAATAGCTGCAATATTGCAA+AGG - chr1.2:81082471-81082490 MS.gene029485:intron 35.0%
CTCCAGTTTATACTAGTTGT+TGG - chr1.2:81081963-81081982 MS.gene029485:intron 35.0%
CTGTAACAAACCTTGTAGTT+TGG - chr1.2:81081895-81081914 MS.gene029485:intron 35.0%
GATCTAACAGAAATGCTCTT+TGG + chr1.2:81081471-81081490 None:intergenic 35.0%
TACAAGTAACAAGTGATGCA+GGG - chr1.2:81082885-81082904 MS.gene029485:intron 35.0%
TTACAAGTAACAAGTGATGC+AGG - chr1.2:81082884-81082903 MS.gene029485:intron 35.0%
TTAGTATGACTGTCAAGGAA+TGG + chr1.2:81083232-81083251 None:intergenic 35.0%
TTATGATCACACCACAATTG+CGG + chr1.2:81082170-81082189 None:intergenic 35.0%
TTATTATGAACGTCAAGGCA+TGG + chr1.2:81083328-81083347 None:intergenic 35.0%
TTATTCTCAATCACACTCGT+CGG + chr1.2:81081420-81081439 None:intergenic 35.0%
! AGCATTGTTGTTAGTTACGA+CGG + chr1.2:81081526-81081545 None:intergenic 35.0%
! CACCGATTAATAACAATGAG+TGG - chr1.2:81083372-81083391 MS.gene029485:CDS 35.0%
! TGAGAATAACGGTGTTGAAA+CGG - chr1.2:81081431-81081450 MS.gene029485:intron 35.0%
!! AAAATTAAGTTGACGCCACA+AGG - chr1.2:81082432-81082451 MS.gene029485:intron 35.0%
AAACCATGGTTGTTTGTGCT+TGG - chr1.2:81082522-81082541 MS.gene029485:intron 40.0%
AAATGGCATCTATGGCCATA+CGG + chr1.2:81083702-81083721 None:intergenic 40.0%
AATTGACAGTGGTCTCCATA+AGG + chr1.2:81083588-81083607 None:intergenic 40.0%
ACAAGTAACAAGTGATGCAG+GGG - chr1.2:81082886-81082905 MS.gene029485:intron 40.0%
AGACTGAGCTCTGTAACAAA+TGG - chr1.2:81083544-81083563 MS.gene029485:CDS 40.0%
AGTCGCCAAAATCAATACCT+TGG - chr1.2:81082821-81082840 MS.gene029485:intron 40.0%
ATGGAAATTTGCAGTGCTGT+TGG - chr1.2:81082782-81082801 MS.gene029485:intron 40.0%
ATTGACAGTGGTCTCCATAA+GGG + chr1.2:81083587-81083606 None:intergenic 40.0%
CTTCTGCAACTCTTTGTTCA+CGG + chr1.2:81081187-81081206 None:intergenic 40.0%
CTTGAATCTTGGAGATGGAA+AGG - chr1.2:81081314-81081333 MS.gene029485:intron 40.0%
CTTTATCCTTACTCTGACCT+TGG - chr1.2:81081030-81081049 MS.gene029485:CDS 40.0%
CTTTCCAGCTTCATTGATCT+GGG + chr1.2:81083771-81083790 None:intergenic 40.0%
GCACTGCAAATTTCCATACA+TGG + chr1.2:81082779-81082798 None:intergenic 40.0%
TCTCTTGTTCTGTAAGTGCA+TGG + chr1.2:81083728-81083747 None:intergenic 40.0%
TCTTTCCAGCTTCATTGATC+TGG + chr1.2:81083772-81083791 None:intergenic 40.0%
TGTAACAAATGGCAGGAAAC+TGG - chr1.2:81083555-81083574 MS.gene029485:CDS 40.0%
TGTGCTTACAACTGTATCAG+TGG + chr1.2:81081586-81081605 None:intergenic 40.0%
TGTTACTATGCTGCAGCAAA+AGG - chr1.2:81083452-81083471 MS.gene029485:CDS 40.0%
TTGTACACAACCATCTCAAG+AGG + chr1.2:81083510-81083529 None:intergenic 40.0%
! GAAAGGAAAACTGTGCTGAT+TGG - chr1.2:81081331-81081350 MS.gene029485:intron 40.0%
! TTGTTACTCACTGCTGACTA+TGG - chr1.2:81083022-81083041 MS.gene029485:intron 40.0%
! TTGTTGGTTAGTAGTGTTGC+AGG - chr1.2:81081979-81081998 MS.gene029485:intron 40.0%
!! AAAAGGTATTTGTGCGAGGA+GGG - chr1.2:81083469-81083488 MS.gene029485:CDS 40.0%
!! ATTAGCTCTAGTTTGCCATG+AGG - chr1.2:81082007-81082026 MS.gene029485:intron 40.0%
!! CAACTTCTTTGGAACTTTCC+AGG + chr1.2:81081131-81081150 None:intergenic 40.0%
!! CAAGGTTTGTTACAGCTAAC+AGG + chr1.2:81081890-81081909 None:intergenic 40.0%
!! GAATCTTCATTGGTTAGCCT+TGG - chr1.2:81083054-81083073 MS.gene029485:CDS 40.0%
!! GATACAGAAGCATCAGGATA+TGG - chr1.2:81081074-81081093 MS.gene029485:CDS 40.0%
!!! ATGGTTTTATCCACTGCTGT+TGG - chr1.2:81082938-81082957 MS.gene029485:intron 40.0%
AAGGGGATAAATGGTTGGAC+TGG - chr1.2:81082859-81082878 MS.gene029485:intron 45.0%
AATGGCATCTATGGCCATAC+GGG + chr1.2:81083701-81083720 None:intergenic 45.0%
ACACCAAGCACAAACAACCA+TGG + chr1.2:81082528-81082547 None:intergenic 45.0%
ACTCCGTTTGATGTGTTGCA+AGG - chr1.2:81081282-81081301 MS.gene029485:intron 45.0%
AGATGGTTGTGTACAACCAG+GGG - chr1.2:81083514-81083533 MS.gene029485:CDS 45.0%
AGCAAGCTTCAAGTAGCCAT+TGG + chr1.2:81081493-81081512 None:intergenic 45.0%
AGTGCCGTAATGAGGGAATT+GGG - chr1.2:81082212-81082231 MS.gene029485:intron 45.0%
ATGACTGTCAAGGAATGGCA+AGG + chr1.2:81083227-81083246 None:intergenic 45.0%
ATGAGTCGTACTCTGTTCCA+AGG + chr1.2:81081050-81081069 None:intergenic 45.0%
CATCTCCAAGATTCAAGCCA+CGG + chr1.2:81081311-81081330 None:intergenic 45.0%
CTGCAGAGCTTCAACTTCTT+TGG + chr1.2:81081142-81081161 None:intergenic 45.0%
CTTGGACACATCTTGTGCTT+GGG - chr1.2:81083072-81083091 MS.gene029485:CDS 45.0%
CTTGTTCTGTAAGTGCATGG+CGG + chr1.2:81083725-81083744 None:intergenic 45.0%
GAGATGGTTGTGTACAACCA+GGG - chr1.2:81083513-81083532 MS.gene029485:CDS 45.0%
GATGTGTATTGTCGTCCTTG+TGG + chr1.2:81082450-81082469 None:intergenic 45.0%
GCTTACAACTGTATCAGTGG+AGG + chr1.2:81081583-81081602 None:intergenic 45.0%
GGTAATGACACAGACAAGCA+AGG + chr1.2:81081869-81081888 None:intergenic 45.0%
GTACAACGAAGCAAACAAGC+CGG + chr1.2:81080944-81080963 None:intergenic 45.0%
GTCAATTCGCTCATGGCATT+GGG - chr1.2:81083601-81083620 MS.gene029485:CDS 45.0%
GTGCCAATGTGCTCTGTATA+TGG - chr1.2:81082085-81082104 MS.gene029485:intron 45.0%
TAAGCCCAGATCAATGAAGC+TGG - chr1.2:81083764-81083783 MS.gene029485:CDS 45.0%
TATGTTTCTCAGTCGTCTCC+TGG - chr1.2:81081110-81081129 MS.gene029485:CDS 45.0%
TCAATTCGCTCATGGCATTG+GGG - chr1.2:81083602-81083621 MS.gene029485:CDS 45.0%
TCTGTTCCAAGGTCAGAGTA+AGG + chr1.2:81081039-81081058 None:intergenic 45.0%
TGAGATGGTTGTGTACAACC+AGG - chr1.2:81083512-81083531 MS.gene029485:CDS 45.0%
TGAGCTCTGTAACAAATGGC+AGG - chr1.2:81083548-81083567 MS.gene029485:CDS 45.0%
TGCACCCAATTCCCTCATTA+CGG + chr1.2:81082219-81082238 None:intergenic 45.0%
TGTCAATTCGCTCATGGCAT+TGG - chr1.2:81083600-81083619 MS.gene029485:CDS 45.0%
TGTTCTCCGTCATCCAAAAG+CGG - chr1.2:81080874-81080893 MS.gene029485:CDS 45.0%
! AGCTCATCTGTTTTGCCTCA+TGG + chr1.2:81082025-81082044 None:intergenic 45.0%
! ATAACGGTGTTGAAACGGAG+AGG - chr1.2:81081436-81081455 MS.gene029485:intron 45.0%
! ATTTGCAGTGCTGTTGGTAG+TGG - chr1.2:81082788-81082807 MS.gene029485:intron 45.0%
! CAAACGGAGTTGATTGAGTC+TGG + chr1.2:81081272-81081291 None:intergenic 45.0%
! TTGTTGTTAGTTACGACGGC+CGG + chr1.2:81081522-81081541 None:intergenic 45.0%
!! AGACCTCAGTTTTGTAGCGT+GGG + chr1.2:81083650-81083669 None:intergenic 45.0%
!! ATGCGTGATACAGAAGCATC+AGG - chr1.2:81081068-81081087 MS.gene029485:CDS 45.0%
!! CAAAAGGTATTTGTGCGAGG+AGG - chr1.2:81083468-81083487 MS.gene029485:CDS 45.0%
!! CAGCAAAAGGTATTTGTGCG+AGG - chr1.2:81083465-81083484 MS.gene029485:CDS 45.0%
!!! ATTTACTATTATTTATTTTA+TGG + chr1.2:81082393-81082412 None:intergenic 5.0%
AACAATGCTTGCCGCACCAA+AGG - chr1.2:81081537-81081556 MS.gene029485:intron 50.0%
AGAAGTTGAAGCTCTGCAGC+AGG - chr1.2:81081143-81081162 MS.gene029485:CDS 50.0%
AGAGGAAGAGCCTCTTGAGA+TGG - chr1.2:81083497-81083516 MS.gene029485:CDS 50.0%
AGCACATTGGCACAGAACAC+AGG + chr1.2:81082078-81082097 None:intergenic 50.0%
AGGATCCGCCACCATTGAAT+CGG + chr1.2:81081245-81081264 None:intergenic 50.0%
AGTTGATTGAGTCTGGACGG+AGG + chr1.2:81081265-81081284 None:intergenic 50.0%
ATCGCATCAAGCCGCAATTG+TGG - chr1.2:81082156-81082175 MS.gene029485:intron 50.0%
CAAAACTGAGGTCTGCAGGA+TGG - chr1.2:81083656-81083675 MS.gene029485:CDS 50.0%
CAGTGCCGTAATGAGGGAAT+TGG - chr1.2:81082211-81082230 MS.gene029485:intron 50.0%
CCAAGCACAAGATGTGTCCA+AGG + chr1.2:81083074-81083093 None:intergenic 50.0%
CCGTCATCCAAAAGCGGTTA+CGG - chr1.2:81080880-81080899 MS.gene029485:CDS 50.0%
CCTTGGACACATCTTGTGCT+TGG - chr1.2:81083071-81083090 MS.gene029485:CDS 50.0%
CGTCGGACTCTCATCAGATT+CGG + chr1.2:81081403-81081422 None:intergenic 50.0%
CGTGGCTTGAATCTTGGAGA+TGG - chr1.2:81081309-81081328 MS.gene029485:intron 50.0%
GAAACTGGCACATGCCCTTA+TGG - chr1.2:81083570-81083589 MS.gene029485:CDS 50.0%
GAAAGCTACACCAACAGCAG+TGG + chr1.2:81082951-81082970 None:intergenic 50.0%
GAAGCTCTGCAGCAGGAAAA+CGG - chr1.2:81081150-81081169 MS.gene029485:CDS 50.0%
GACCACTGTCAATTCGCTCA+TGG - chr1.2:81083594-81083613 MS.gene029485:CDS 50.0%
GATGTGTTGCAAGGTTTCCG+TGG - chr1.2:81081291-81081310 MS.gene029485:intron 50.0%
GCAACTCTTTGTTCACGGCA+CGG + chr1.2:81081182-81081201 None:intergenic 50.0%
GCGATTGCAGACAATACCGT+GGG + chr1.2:81082141-81082160 None:intergenic 50.0%
GCTACAAAACTGAGGTCTGC+AGG - chr1.2:81083652-81083671 MS.gene029485:CDS 50.0%
GGTTTCCGTGGCTTGAATCT+TGG - chr1.2:81081303-81081322 MS.gene029485:intron 50.0%
TCCAAAAGCGGTTACGGCAT+CGG - chr1.2:81080886-81080905 MS.gene029485:CDS 50.0%
TGCCATGAGCGAATTGACAG+TGG + chr1.2:81083599-81083618 None:intergenic 50.0%
TGCGATTGCAGACAATACCG+TGG + chr1.2:81082142-81082161 None:intergenic 50.0%
! CATGGCGGAAATGGCATCTA+TGG + chr1.2:81083710-81083729 None:intergenic 50.0%
! CGGAGTTGATTGAGTCTGGA+CGG + chr1.2:81081268-81081287 None:intergenic 50.0%
! CTGGAGAACCGATTCAATGG+TGG - chr1.2:81081234-81081253 MS.gene029485:intron 50.0%
! CTGTAAGTGCATGGCGGAAA+TGG + chr1.2:81083719-81083738 None:intergenic 50.0%
! GAGAACCGATTCAATGGTGG+CGG - chr1.2:81081237-81081256 MS.gene029485:intron 50.0%
! TCGCTGGAGAACCGATTCAA+TGG - chr1.2:81081231-81081250 MS.gene029485:intron 50.0%
!! CAGACCTCAGTTTTGTAGCG+TGG + chr1.2:81083651-81083670 None:intergenic 50.0%
!! CCGTAACCGCTTTTGGATGA+CGG + chr1.2:81080883-81080902 None:intergenic 50.0%
!!! GCGGCCGCATTTTAAAACCA+TGG - chr1.2:81082508-81082527 MS.gene029485:intron 50.0%
AAAGGCAGCGACTGCAACAG+CGG - chr1.2:81082489-81082508 MS.gene029485:intron 55.0%
ACGTCGCAGTGCCGTAATGA+GGG - chr1.2:81082205-81082224 MS.gene029485:intron 55.0%
CCACCCACGCTACAAAACTG+AGG - chr1.2:81083644-81083663 MS.gene029485:CDS 55.0%
CCAGCGAAGCTTGAACGAGA+AGG + chr1.2:81081218-81081237 None:intergenic 55.0%
CCTTCTCGTTCAAGCTTCGC+TGG - chr1.2:81081215-81081234 MS.gene029485:intron 55.0%
CTGTATCAGTGGAGGAAGCG+CGG + chr1.2:81081575-81081594 None:intergenic 55.0%
CTGTCAAGGAATGGCAAGGC+AGG + chr1.2:81083223-81083242 None:intergenic 55.0%
GATCTTCCATCATCGCTCGC+CGG - chr1.2:81080979-81080998 MS.gene029485:CDS 55.0%
GCTCAGTCTTGAACATCCCC+TGG + chr1.2:81083533-81083552 None:intergenic 55.0%
TCTGCAGGATGGTCCTTGCT+GGG - chr1.2:81083667-81083686 MS.gene029485:CDS 55.0%
TTGTTAGTTACGACGGCCGG+AGG + chr1.2:81081519-81081538 None:intergenic 55.0%
! TGTATCAGTGGAGGAAGCGC+GGG + chr1.2:81081574-81081593 None:intergenic 55.0%
!! CAGTTTTGTAGCGTGGGTGG+CGG + chr1.2:81083644-81083663 None:intergenic 55.0%
!! CCTCAGTTTTGTAGCGTGGG+TGG + chr1.2:81083647-81083666 None:intergenic 55.0%
AGCGAGTAGCGCCTTTGGTG+CGG + chr1.2:81081551-81081570 None:intergenic 60.0%
CACGTCGCAGTGCCGTAATG+AGG - chr1.2:81082204-81082223 MS.gene029485:intron 60.0%
CTCATTACGGCACTGCGACG+TGG + chr1.2:81082206-81082225 None:intergenic 60.0%
CTCGCCGGAAACAGCAGCAA+CGG - chr1.2:81080994-81081013 MS.gene029485:CDS 60.0%
CTGCAGGATGGTCCTTGCTG+GGG - chr1.2:81083668-81083687 MS.gene029485:CDS 60.0%
CTGTTTCCGGCGAGCGATGA+TGG + chr1.2:81080988-81081007 None:intergenic 60.0%
GACAATACCGTGGGCGCATC+AGG + chr1.2:81082132-81082151 None:intergenic 60.0%
GGCGTTCGACTGCATGTGAC+CGG + chr1.2:81080923-81080942 None:intergenic 60.0%
GGTCACATGCAGTCGAACGC+CGG - chr1.2:81080922-81080941 MS.gene029485:CDS 60.0%
GTCTGCAGGATGGTCCTTGC+TGG - chr1.2:81083666-81083685 MS.gene029485:CDS 60.0%
TGCATCACCTGATGCGCCCA+CGG - chr1.2:81082122-81082141 MS.gene029485:intron 60.0%
TGTGCGAGGAGGGCAGAAAG+AGG - chr1.2:81083479-81083498 MS.gene029485:CDS 60.0%
TTAGTTACGACGGCCGGAGG+CGG + chr1.2:81081516-81081535 None:intergenic 60.0%
! CTTGAAGCTTGCTCCGCCTC+CGG - chr1.2:81081500-81081519 MS.gene029485:intron 60.0%
! GCCGATGCCGTAACCGCTTT+TGG + chr1.2:81080890-81080909 None:intergenic 60.0%
ACGGGCACACAACCCCAGCA+AGG + chr1.2:81083683-81083702 None:intergenic 65.0%
GCCGGAAACAGCAGCAACGG+CGG - chr1.2:81080997-81081016 MS.gene029485:CDS 65.0%
GCTGGGGTTGTGTGCCCGTA+TGG - chr1.2:81083684-81083703 MS.gene029485:CDS 65.0%
GGAAACAGCAGCAACGGCGG+CGG - chr1.2:81081000-81081019 MS.gene029485:CDS 65.0%
!! AGCGTGGGTGGCGGATCACT+GGG + chr1.2:81083635-81083654 None:intergenic 65.0%
!! GCCGCCGTTGCTGCTGTTTC+CGG + chr1.2:81081001-81081020 None:intergenic 65.0%
!! GGCATCGGCGCAAACAACGC+CGG - chr1.2:81080901-81080920 MS.gene029485:CDS 65.0%
!! TAGCGTGGGTGGCGGATCAC+TGG + chr1.2:81083636-81083655 None:intergenic 65.0%
GCGGGAGCGAGTAGCGCCTT+TGG + chr1.2:81081556-81081575 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 81080856 81083794 81080856 ID=MS.gene029485
chr1.2 mRNA 81080856 81083794 81080856 ID=MS.gene029485.t1;Parent=MS.gene029485
chr1.2 exon 81083054 81083794 81083054 ID=MS.gene029485.t1.exon1;Parent=MS.gene029485.t1
chr1.2 CDS 81083054 81083794 81083054 ID=cds.MS.gene029485.t1;Parent=MS.gene029485.t1
chr1.2 exon 81080856 81081182 81080856 ID=MS.gene029485.t1.exon2;Parent=MS.gene029485.t1
chr1.2 CDS 81080856 81081182 81080856 ID=cds.MS.gene029485.t1;Parent=MS.gene029485.t1
Gene Sequence

>MS.gene029485

ATGCATAACAAGGACGTTTGTTCTCCGTCATCCAAAAGCGGTTACGGCATCGGCGCAAACAACGCCGGTCACATGCAGTCGAACGCCGGCTTGTTTGCTTCGTTGTACTCTGCTCTGAGTAATGATCTTCCATCATCGCTCGCCGGAAACAGCAGCAACGGCGGCGGATTTTCGCTTTATCCTTACTCTGACCTTGGAACAGAGTACGACTCATGCGTGATACAGAAGCATCAGGATATGGTGAATCGTCACAGTATGTTTCTCAGTCGTCTCCTGGAAAGTTCCAAAGAAGTTGAAGCTCTGCAGCAGGAAAACGGACAGCTCCGTGCCGTGAACAAAGAGTTGCAGAAGAATCTGAACCTTCTCGTTCAAGCTTCGCTGGAGAACCGATTCAATGGTGGCGGATCCTCCGTCCAGACTCAATCAACTCCGTTTGATGTGTTGCAAGGTTTCCGTGGCTTGAATCTTGGAGATGGAAAGGAAAACTGTGCTGATTGGAATAGCAATAACATCAATAATAATAACAAGGAGTTGCAGGAAGCTTCCGAATCTGATGAGAGTCCGACGAGTGTGATTGAGAATAACGGTGTTGAAACGGAGAGGTTCTCGCTTCCAAAGAGCATTTCTGTTAGATCCAATGGCTACTTGAAGCTTGCTCCGCCTCCGGCCGTCGTAACTAACAACAATGCTTGCCGCACCAAAGGCGCTACTCGCTCCCGCGCTTCCTCCACTGATACAGTTGTAAGCACACGCACGTTATGATTGCTTTTATTTTATTTAATTAATTAATCAATTAATTATAATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTTATTTTATTTTATTATTATTGCTGCTATTGTTATTGTGATCTTAGCTTAATTTAATGTGTAATTATTGCTTTTATAGTTATAAACCAAATTTGACTTGCTTGTTAGTAGCTAATTATTTTCCTTCCAAATGAAGTAAAACCTTGCTTGTCTGTGTCATTACCTGTTAGCTGTAACAAACCTTGTAGTTTGGTTGTTTTTTCATTTGCGTGTGCTTTTTGCAAAGATGCAAGTTCGACTCCAGTTTATACTAGTTGTTGGTTAGTAGTGTTGCAGGACCAAATTAGCTCTAGTTTGCCATGAGGCAAAACAGATGAGCTAAACTTTGAAGCCTTTTTGTGACATTTTAACCTGTGTTCTGTGCCAATGTGCTCTGTATATGGTTTCAAATTGCGGATGCATCACCTGATGCGCCCACGGTATTGTCTGCAATCGCATCAAGCCGCAATTGTGGTGTGATCATAAATCACATTTGAAACCACGTCGCAGTGCCGTAATGAGGGAATTGGGTGCAGAAAACTAGTGATCACACAATTTATTTTTATACTTTACTTTATCTTTATATCATAGATTTAGAGAGTATTTTAAAGAACTAGCGATATTTTAAACTAATAGATGGCTGATATGAGATAAAGATTTTAGAGAGGAAACAGAAGATACATGAGCCATAAAATAAATAATAGTAAATTATAGTATTTATGCTAATAAAAATTAAGTTGACGCCACAAGGACGACAATACACATCGCAATAGCTGCAATATTGCAAAGGCAGCGACTGCAACAGCGGCCGCATTTTAAAACCATGGTTGTTTGTGCTTGGTGTCATGTTCCAATTAAGTTAAATTTTGATGCAGCCATACAGTTTTTACATTATAATTAAGGTGGTTAGATAATCTTTCTCACACTTCTCAATAAACTCTTGAAATAATATATAGAACTAGCAAGACTAGGAAGATTGATGAGTCATACAATGTCTACATTATAGGAGTATTTAAGAGGTCAAATATATACTATTTTTCTACATTTCTGAATTTTAAGACATTTATGATAGCCATGTATGGAAATTTGCAGTGCTGTTGGTAGTGGTTTGTATTTTAGTCGCCAAAATCAATACCTTGGTTTTTCTAAGTTTTAAAGGGGATAAATGGTTGGACTGGTTTTACAAGTAACAAGTGATGCAGGGGATGCAGAATATGTCACTCAATTTCTTTTCATGGTTTTATCCACTGCTGTTGGTGTAGCTTTCAATAGACGTATAGTGTGCTGCTTATAGTTTGTCTCCATTTAGTTATTTGTTTTGTTACTCACTGCTGACTATGGTAATGTCTTGAATCTTCATTGGTTAGCCTTGGACACATCTTGTGCTTGGGATTAGCCTCTATTATTAATATATGCTTTGATTTATTTATGTTGAAAATAAGGACAATACTTCGAGATAAAGATGAATGGATGTAAATAATTGTGTTCAATTTTGAAATGGTGTTTTCTTGAATGTCCTGCCTTGCCATTCCTTGACAGTCATACTAATGTTTCTTTCATCTTGACTGATTGTATTTTTCATCTTATTGTTTTTTTTATTTGATTCATATTCAATGCTCTGCCATGCCTTGACGTTCATAATAATGTAAAAGTAGATTGTAGATGCATCACCGATTAATAACAATGAGTGGACATTATATAGATTTGATTGTTGATATTCGGAAGAATTGATCATTGTTGAAATTTTGTGTTACTATGCTGCAGCAAAAGGTATTTGTGCGAGGAGGGCAGAAAGAGGAAGAGCCTCTTGAGATGGTTGTGTACAACCAGGGGATGTTCAAGACTGAGCTCTGTAACAAATGGCAGGAAACTGGCACATGCCCTTATGGAGACCACTGTCAATTCGCTCATGGCATTGGGGAGCTTCGCCCAGTGATCCGCCACCCACGCTACAAAACTGAGGTCTGCAGGATGGTCCTTGCTGGGGTTGTGTGCCCGTATGGCCATAGATGCCATTTCCGCCATGCACTTACAGAACAAGAGAAAGCTATGTCGCAATCTAAGCCCAGATCAATGAAGCTGGAAAGATGA
Protein sequence

>MS.gene029485.t1

MHNKDVCSPSSKSGYGIGANNAGHMQSNAGLFASLYSALSNDLPSSLAGNSSNGGGFSLYPYSDLGTEYDSCVIQKHQDMVNRHSMFLSRLLESSKEVEALQQENGQLRAVNKELQKNLNLLVQASLENRFNGGGSSVQTQSTPFDVLQGFRGLNLGDGKENCADWNSNNINNNNKELQEASESDESPTSVIENNGVETERFSLPKSISVRSNGYLKLAPPPAVVTNNNACRTKGATRSRASSTDTVQKVFVRGGQKEEEPLEMVVYNQGMFKTELCNKWQETGTCPYGDHCQFAHGIGELRPVIRHPRYKTEVCRMVLAGVVCPYGHRCHFRHALTEQEKAMSQSKPRSMKLER