Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029624.t1 | RHN55623.1 | 99.4 | 162 | 1 | 0 | 1 | 162 | 1 | 162 | 5.80E-71 | 276.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029624.t1 | Q76EJ0 | 58.9 | 146 | 53 | 5 | 9 | 151 | 1 | 142 | 1.2e-37 | 157.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029624.t1 | A0A396HT01 | 99.4 | 162 | 1 | 0 | 1 | 162 | 1 | 162 | 4.2e-71 | 276.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene029624.t1 | TF | C2C2-YABBY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029624.t1 | MTR_5g046230 | 96.815 | 157 | 5 | 0 | 1 | 157 | 1 | 157 | 9.42e-111 | 314 |
| MS.gene029624.t1 | MTR_4g114730 | 43.030 | 165 | 65 | 4 | 8 | 155 | 5 | 157 | 2.66e-38 | 129 |
| MS.gene029624.t1 | MTR_2g087740 | 46.784 | 171 | 73 | 6 | 7 | 161 | 1 | 169 | 5.20e-37 | 126 |
| MS.gene029624.t1 | MTR_4g050300 | 41.718 | 163 | 83 | 3 | 7 | 161 | 1 | 159 | 6.16e-36 | 123 |
| MS.gene029624.t1 | MTR_4g025900 | 44.025 | 159 | 62 | 4 | 19 | 155 | 25 | 178 | 1.05e-32 | 116 |
| MS.gene029624.t1 | MTR_4g101660 | 44.785 | 163 | 72 | 3 | 10 | 155 | 12 | 173 | 3.34e-32 | 114 |
| MS.gene029624.t1 | MTR_5g034030 | 40.000 | 155 | 64 | 3 | 17 | 151 | 12 | 157 | 6.60e-30 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029624.t1 | AT1G69180 | 64.596 | 161 | 50 | 4 | 1 | 157 | 1 | 158 | 2.66e-55 | 172 |
| MS.gene029624.t1 | AT1G08465 | 46.875 | 160 | 66 | 5 | 15 | 161 | 6 | 159 | 4.15e-37 | 126 |
| MS.gene029624.t1 | AT2G26580 | 46.939 | 147 | 65 | 3 | 15 | 155 | 7 | 146 | 5.73e-36 | 122 |
| MS.gene029624.t1 | AT2G26580 | 46.939 | 147 | 65 | 3 | 15 | 155 | 7 | 146 | 5.73e-36 | 122 |
| MS.gene029624.t1 | AT2G45190 | 46.429 | 168 | 66 | 7 | 10 | 155 | 17 | 182 | 1.25e-34 | 121 |
| MS.gene029624.t1 | AT4G00180 | 37.968 | 187 | 78 | 5 | 10 | 161 | 17 | 200 | 1.64e-28 | 105 |
| MS.gene029624.t1 | AT1G23420 | 36.478 | 159 | 82 | 3 | 18 | 157 | 20 | 178 | 2.05e-25 | 97.4 |
| MS.gene029624.t1 | AT1G23420 | 36.709 | 158 | 81 | 3 | 19 | 157 | 52 | 209 | 3.77e-25 | 97.4 |
| MS.gene029624.t1 | AT4G00180 | 33.918 | 171 | 76 | 4 | 26 | 161 | 1 | 169 | 1.29e-19 | 82.0 |
| MS.gene029624.t1 | AT2G45190 | 70.909 | 55 | 15 | 1 | 101 | 155 | 65 | 118 | 3.55e-19 | 79.7 |
Find 34 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATGGTAACTTTCTCTTCA+TGG | 0.253602 | 5.4:-36466532 | None:intergenic |
| ACAACAAATGGCGGTGGTCT+AGG | 0.360978 | 5.4:-36467270 | None:intergenic |
| TGCTTACGTTTGACAACAAA+TGG | 0.370125 | 5.4:-36467282 | None:intergenic |
| CAACAAATGGCGGTGGTCTA+GGG | 0.442200 | 5.4:-36467269 | None:intergenic |
| GGAAGACGGTGCTTCTTCTC+AGG | 0.456267 | 5.4:-36467453 | None:intergenic |
| ATTGCACTTACTTCATGAAA+CGG | 0.485516 | 5.4:-36467488 | None:intergenic |
| TGATCACACCCTCAGTCTCC+AGG | 0.486490 | 5.4:+36466961 | MS.gene029624:CDS |
| AGAGAAAGTTACCATGGACT+TGG | 0.516644 | 5.4:+36466538 | MS.gene029624:CDS |
| CACTGAAAGCTTCTCGATGT+GGG | 0.523718 | 5.4:-36467891 | None:intergenic |
| TTTGGAACTTGGTGGTCTTG+TGG | 0.524145 | 5.4:-36466927 | None:intergenic |
| GCTTCTCGATGTGGGATCTG+AGG | 0.524810 | 5.4:-36467883 | None:intergenic |
| CCCTAATAAGAAAATAAAAG+AGG | 0.527545 | 5.4:-36467943 | None:intergenic |
| CTTCTCGATGTGGGATCTGA+GGG | 0.529903 | 5.4:-36467882 | None:intergenic |
| ATGAAACGGTTGTATGCAGA+TGG | 0.547267 | 5.4:-36467474 | None:intergenic |
| GCACTGAAAGCTTCTCGATG+TGG | 0.561704 | 5.4:-36467892 | None:intergenic |
| TCGATGTGGGATCTGAGGGT+TGG | 0.564009 | 5.4:-36467878 | None:intergenic |
| ATTATTGAACCTGGAGACTG+AGG | 0.580682 | 5.4:-36466970 | None:intergenic |
| TAACAGAGGTGTTGATCAGA+TGG | 0.588546 | 5.4:-36466566 | None:intergenic |
| AGTGACTCAGTTGATGTAGT+TGG | 0.590905 | 5.4:-36467243 | None:intergenic |
| AACAAATGGCGGTGGTCTAG+GGG | 0.595027 | 5.4:-36467268 | None:intergenic |
| GTTGGTGAAGAAGAAGATGA+TGG | 0.609575 | 5.4:-36467225 | None:intergenic |
| CGTTTGACAACAAATGGCGG+TGG | 0.615312 | 5.4:-36467276 | None:intergenic |
| AGATGGTGGAACCAAGTCCA+TGG | 0.626957 | 5.4:-36466549 | None:intergenic |
| CCATGAAGAGAAAGTTACCA+TGG | 0.629385 | 5.4:+36466532 | MS.gene029624:CDS |
| GTATCTAGCAGCCTCTTGCA+TGG | 0.634316 | 5.4:-36466860 | None:intergenic |
| GGTTGTATGCAGATGGAAGA+CGG | 0.643999 | 5.4:-36467467 | None:intergenic |
| GTGAAGAAGAAGATGATGGT+TGG | 0.655028 | 5.4:-36467221 | None:intergenic |
| CAGAGGTGTTGATCAGATGG+TGG | 0.661444 | 5.4:-36466563 | None:intergenic |
| AGGTTGGCATACCATGCAAG+AGG | 0.666035 | 5.4:+36466849 | MS.gene029624:intron |
| TTATTGAACCTGGAGACTGA+GGG | 0.671255 | 5.4:-36466969 | None:intergenic |
| TTACGTTTGACAACAAATGG+CGG | 0.678472 | 5.4:-36467279 | None:intergenic |
| GATACTGTGACAGTGAAGTG+TGG | 0.709816 | 5.4:+36466878 | MS.gene029624:CDS |
| AGTTGCAACGAACGTAACAG+AGG | 0.710301 | 5.4:-36466580 | None:intergenic |
| GTCTTGTGGTGAGAAAAGAG+AGG | 0.744847 | 5.4:-36466913 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTAATTAATTAATAAAA+AGG | - | chr5.4:36467137-36467156 | None:intergenic | 0.0% |
| !! | TGATATATGAATAATATTAA+AGG | - | chr5.4:36466640-36466659 | None:intergenic | 10.0% |
| !!! | AAATTTTTCTAAAATATGTA+GGG | + | chr5.4:36466805-36466824 | MS.gene029624:intron | 10.0% |
| !!! | TAAATTTTTCTAAAATATGT+AGG | + | chr5.4:36466804-36466823 | MS.gene029624:intron | 10.0% |
| !!! | TATAGTATATATTTTAAACA+GGG | - | chr5.4:36467633-36467652 | None:intergenic | 10.0% |
| !! | CTAAAATTTCTCTTATAAAT+AGG | - | chr5.4:36467670-36467689 | None:intergenic | 15.0% |
| !!! | ATAAAAAATGCAAACATTTT+TGG | - | chr5.4:36467923-36467942 | None:intergenic | 15.0% |
| !!! | GTATAGTATATATTTTAAAC+AGG | - | chr5.4:36467634-36467653 | None:intergenic | 15.0% |
| !!! | TTTTTTATTAGCTACATATA+TGG | + | chr5.4:36466758-36466777 | MS.gene029624:intron | 15.0% |
| !! | TCAACAAAAATTGATGTATT+TGG | + | chr5.4:36467690-36467709 | MS.gene029624:intron | 20.0% |
| !! | TTTAATACTGTCTCTAAATT+TGG | + | chr5.4:36466719-36466738 | MS.gene029624:intron | 20.0% |
| !!! | ATATATTTTAAACAGGGTAA+GGG | - | chr5.4:36467627-36467646 | None:intergenic | 20.0% |
| !!! | ATGATGAGTTTTATATGTAT+TGG | + | chr5.4:36467110-36467129 | MS.gene029624:intron | 20.0% |
| !!! | CATCTATATTTTTTGTTTTG+AGG | + | chr5.4:36467032-36467051 | MS.gene029624:intron | 20.0% |
| !!! | TATATATTTTAAACAGGGTA+AGG | - | chr5.4:36467628-36467647 | None:intergenic | 20.0% |
| ! | ATTTCTCTTATAAATAGGAC+CGG | - | chr5.4:36467665-36467684 | None:intergenic | 25.0% |
| ! | CCTAACTTTCTCTTATAAAT+AGG | + | chr5.4:36467749-36467768 | MS.gene029624:intron | 25.0% |
| ! | CCTATTTATAAGAGAAAGTT+AGG | - | chr5.4:36467752-36467771 | None:intergenic | 25.0% |
| ! | CTAAACAAAATTATTGAACC+TGG | - | chr5.4:36466982-36467001 | None:intergenic | 25.0% |
| ATTGCACTTACTTCATGAAA+CGG | - | chr5.4:36467491-36467510 | None:intergenic | 30.0% | |
| CTTTCTCTTATAAATAGGAC+CGG | + | chr5.4:36467754-36467773 | MS.gene029624:intron | 30.0% | |
| GATATTTCCTTAGAGAGTAA+TGG | - | chr5.4:36467318-36467337 | None:intergenic | 30.0% | |
| TTAGAGAGTAATGGTGAAAA+GGG | - | chr5.4:36467309-36467328 | None:intergenic | 30.0% | |
| ! | AGTTAACAAGTTGAGAATGA+TGG | - | chr5.4:36466668-36466687 | None:intergenic | 30.0% |
| !! | AATACTTTTGTTTGTAGCAG+GGG | + | chr5.4:36467178-36467197 | MS.gene029624:intron | 30.0% |
| !! | AGAATACTTTTGTTTGTAGC+AGG | + | chr5.4:36467176-36467195 | MS.gene029624:intron | 30.0% |
| !! | ATACTTTTTATCTGTCAGAG+AGG | + | chr5.4:36467834-36467853 | MS.gene029624:intron | 30.0% |
| !! | GAATACTTTTGTTTGTAGCA+GGG | + | chr5.4:36467177-36467196 | MS.gene029624:intron | 30.0% |
| !! | GTGATCAACAGTTTGATTTT+TGG | - | chr5.4:36466948-36466967 | None:intergenic | 30.0% |
| !! | GTTTGTTATGTTATGTAGGT+TGG | + | chr5.4:36466833-36466852 | MS.gene029624:intron | 30.0% |
| !! | TGTGGTTTGTTATGTTATGT+AGG | + | chr5.4:36466829-36466848 | MS.gene029624:intron | 30.0% |
| !! | TTCTAAAATATGTAGGGTTG+TGG | + | chr5.4:36466811-36466830 | MS.gene029624:intron | 30.0% |
| !!! | ACAGTTTGATTTTTGGAACT+TGG | - | chr5.4:36466941-36466960 | None:intergenic | 30.0% |
| AACCAACCATAACTACAAGA+TGG | + | chr5.4:36467586-36467605 | MS.gene029624:intron | 35.0% | |
| AGTAAACTATATACTCCCTC+CGG | - | chr5.4:36467776-36467795 | None:intergenic | 35.0% | |
| ATATACTATACTACTCCCTC+CGG | + | chr5.4:36467643-36467662 | MS.gene029624:intron | 35.0% | |
| CTTAGAGAGTAATGGTGAAA+AGG | - | chr5.4:36467310-36467329 | None:intergenic | 35.0% | |
| GGGATTTACAGTAGCAAAAA+AGG | + | chr5.4:36467198-36467217 | MS.gene029624:CDS | 35.0% | |
| GTGTTACCATCTTGTAGTTA+TGG | - | chr5.4:36467595-36467614 | None:intergenic | 35.0% | |
| TACCATCTTGTAGTTATGGT+TGG | - | chr5.4:36467591-36467610 | None:intergenic | 35.0% | |
| TGCTTACGTTTGACAACAAA+TGG | - | chr5.4:36467285-36467304 | None:intergenic | 35.0% | |
| TTACGTTTGACAACAAATGG+CGG | - | chr5.4:36467282-36467301 | None:intergenic | 35.0% | |
| ! | AACAAGTTGAGAATGATGGA+TGG | - | chr5.4:36466664-36466683 | None:intergenic | 35.0% |
| ! | CTTTTCACCATTACTCTCTA+AGG | + | chr5.4:36467308-36467327 | MS.gene029624:intron | 35.0% |
| !!! | GTTTGATTTTTGGAACTTGG+TGG | - | chr5.4:36466938-36466957 | None:intergenic | 35.0% |
| AGAGAAAGTTACCATGGACT+TGG | + | chr5.4:36466538-36466557 | MS.gene029624:CDS | 40.0% | |
| ATGAAACGGTTGTATGCAGA+TGG | - | chr5.4:36467477-36467496 | None:intergenic | 40.0% | |
| ATTATTGAACCTGGAGACTG+AGG | - | chr5.4:36466973-36466992 | None:intergenic | 40.0% | |
| CCATGAAGAGAAAGTTACCA+TGG | + | chr5.4:36466532-36466551 | MS.gene029624:CDS | 40.0% | |
| CCATGGTAACTTTCTCTTCA+TGG | - | chr5.4:36466535-36466554 | None:intergenic | 40.0% | |
| CTCTTATAAATAGGACCGGA+GGG | + | chr5.4:36467758-36467777 | MS.gene029624:intron | 40.0% | |
| CTCTTATAAATAGGACCGGA+GGG | - | chr5.4:36467777-36467758 | None:intergenic | 40.0% | |
| GGTGAAGTTTACATACAGCT+AGG | - | chr5.4:36466619-36466638 | None:intergenic | 40.0% | |
| GTTAGGTCAACAAAAGTCGA+TGG | - | chr5.4:36467735-36467754 | None:intergenic | 40.0% | |
| TCTCTTATAAATAGGACCGG+AGG | + | chr5.4:36467757-36467776 | MS.gene029624:intron | 40.0% | |
| TCTCTTATAAATAGGACCGG+AGG | - | chr5.4:36467776-36467757 | None:intergenic | 40.0% | |
| TTATTGAACCTGGAGACTGA+GGG | - | chr5.4:36466972-36466991 | None:intergenic | 40.0% | |
| ! | AGTGACTCAGTTGATGTAGT+TGG | - | chr5.4:36467246-36467265 | None:intergenic | 40.0% |
| ! | GTGAAGAAGAAGATGATGGT+TGG | - | chr5.4:36467224-36467243 | None:intergenic | 40.0% |
| ! | GTTGGTGAAGAAGAAGATGA+TGG | - | chr5.4:36467228-36467247 | None:intergenic | 40.0% |
| !! | TAACAGAGGTGTTGATCAGA+TGG | - | chr5.4:36466569-36466588 | None:intergenic | 40.0% |
| AGTTGCAACGAACGTAACAG+AGG | - | chr5.4:36466583-36466602 | None:intergenic | 45.0% | |
| CACTGAAAGCTTCTCGATGT+GGG | - | chr5.4:36467894-36467913 | None:intergenic | 45.0% | |
| GATACTGTGACAGTGAAGTG+TGG | + | chr5.4:36466878-36466897 | MS.gene029624:CDS | 45.0% | |
| GGTTGTATGCAGATGGAAGA+CGG | - | chr5.4:36467470-36467489 | None:intergenic | 45.0% | |
| GTCTTGTGGTGAGAAAAGAG+AGG | - | chr5.4:36466916-36466935 | None:intergenic | 45.0% | |
| ! | TTTGGAACTTGGTGGTCTTG+TGG | - | chr5.4:36466930-36466949 | None:intergenic | 45.0% |
| AACAAATGGCGGTGGTCTAG+GGG | - | chr5.4:36467271-36467290 | None:intergenic | 50.0% | |
| ACAACAAATGGCGGTGGTCT+AGG | - | chr5.4:36467273-36467292 | None:intergenic | 50.0% | |
| AGGTTGGCATACCATGCAAG+AGG | + | chr5.4:36466849-36466868 | MS.gene029624:intron | 50.0% | |
| CAACAAATGGCGGTGGTCTA+GGG | - | chr5.4:36467272-36467291 | None:intergenic | 50.0% | |
| CGTTTGACAACAAATGGCGG+TGG | - | chr5.4:36467279-36467298 | None:intergenic | 50.0% | |
| CTTCTCGATGTGGGATCTGA+GGG | - | chr5.4:36467885-36467904 | None:intergenic | 50.0% | |
| GCACTGAAAGCTTCTCGATG+TGG | - | chr5.4:36467895-36467914 | None:intergenic | 50.0% | |
| GTATCTAGCAGCCTCTTGCA+TGG | - | chr5.4:36466863-36466882 | None:intergenic | 50.0% | |
| !! | AGATGGTGGAACCAAGTCCA+TGG | - | chr5.4:36466552-36466571 | None:intergenic | 50.0% |
| !! | CAGAGGTGTTGATCAGATGG+TGG | - | chr5.4:36466566-36466585 | None:intergenic | 50.0% |
| GCTTCTCGATGTGGGATCTG+AGG | - | chr5.4:36467886-36467905 | None:intergenic | 55.0% | |
| GGAAGACGGTGCTTCTTCTC+AGG | - | chr5.4:36467456-36467475 | None:intergenic | 55.0% | |
| TCGATGTGGGATCTGAGGGT+TGG | - | chr5.4:36467881-36467900 | None:intergenic | 55.0% | |
| TGATCACACCCTCAGTCTCC+AGG | + | chr5.4:36466961-36466980 | MS.gene029624:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 36466527 | 36467963 | 36466527 | ID=MS.gene029624 |
| chr5.4 | mRNA | 36466527 | 36467963 | 36466527 | ID=MS.gene029624.t1;Parent=MS.gene029624 |
| chr5.4 | exon | 36466527 | 36466622 | 36466527 | ID=MS.gene029624.t1.exon1;Parent=MS.gene029624.t1 |
| chr5.4 | CDS | 36466527 | 36466622 | 36466527 | ID=cds.MS.gene029624.t1;Parent=MS.gene029624.t1 |
| chr5.4 | exon | 36466851 | 36466982 | 36466851 | ID=MS.gene029624.t1.exon2;Parent=MS.gene029624.t1 |
| chr5.4 | CDS | 36466851 | 36466982 | 36466851 | ID=cds.MS.gene029624.t1;Parent=MS.gene029624.t1 |
| chr5.4 | exon | 36467198 | 36467297 | 36467198 | ID=MS.gene029624.t1.exon3;Parent=MS.gene029624.t1 |
| chr5.4 | CDS | 36467198 | 36467297 | 36467198 | ID=cds.MS.gene029624.t1;Parent=MS.gene029624.t1 |
| chr5.4 | exon | 36467451 | 36467499 | 36467451 | ID=MS.gene029624.t1.exon4;Parent=MS.gene029624.t1 |
| chr5.4 | CDS | 36467451 | 36467499 | 36467451 | ID=cds.MS.gene029624.t1;Parent=MS.gene029624.t1 |
| chr5.4 | exon | 36467852 | 36467963 | 36467852 | ID=MS.gene029624.t1.exon5;Parent=MS.gene029624.t1 |
| chr5.4 | CDS | 36467852 | 36467963 | 36467852 | ID=cds.MS.gene029624.t1;Parent=MS.gene029624.t1 |
| Gene Sequence |
| Protein sequence |
