Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029651.t1 | XP_003614286.1 | 95.5 | 156 | 7 | 0 | 1 | 156 | 1 | 156 | 1.40E-74 | 288.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029651.t1 | Q9FJK3 | 47.4 | 156 | 81 | 1 | 1 | 155 | 1 | 156 | 3.7e-31 | 136.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029651.t1 | G7JY85 | 95.5 | 156 | 7 | 0 | 1 | 156 | 1 | 156 | 1.0e-74 | 288.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene029651.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029651.t1 | MTR_5g047580 | 95.513 | 156 | 7 | 0 | 1 | 156 | 1 | 156 | 2.64e-108 | 305 |
| MS.gene029651.t1 | MTR_1g084950 | 85.897 | 156 | 22 | 0 | 1 | 156 | 1 | 156 | 9.98e-88 | 253 |
| MS.gene029651.t1 | MTR_5g047560 | 81.410 | 156 | 13 | 1 | 1 | 156 | 1 | 140 | 8.27e-87 | 250 |
| MS.gene029651.t1 | MTR_7g011950 | 75.000 | 156 | 39 | 0 | 1 | 156 | 1 | 156 | 1.05e-75 | 223 |
| MS.gene029651.t1 | MTR_4g028720 | 75.000 | 156 | 39 | 0 | 1 | 156 | 1 | 156 | 4.86e-75 | 221 |
| MS.gene029651.t1 | MTR_1g077390 | 68.590 | 156 | 49 | 0 | 1 | 156 | 1 | 156 | 1.36e-74 | 220 |
| MS.gene029651.t1 | MTR_1g077320 | 72.436 | 156 | 43 | 0 | 1 | 156 | 1 | 156 | 3.02e-73 | 216 |
| MS.gene029651.t1 | MTR_1g077300 | 71.154 | 156 | 43 | 1 | 1 | 156 | 1 | 154 | 2.17e-67 | 202 |
| MS.gene029651.t1 | MTR_1g090783 | 64.052 | 153 | 55 | 0 | 3 | 155 | 2 | 154 | 1.37e-65 | 197 |
| MS.gene029651.t1 | MTR_1g090697 | 58.710 | 155 | 64 | 0 | 1 | 155 | 1 | 155 | 1.92e-61 | 186 |
| MS.gene029651.t1 | MTR_1g090710 | 56.410 | 156 | 67 | 1 | 1 | 155 | 1 | 156 | 7.06e-59 | 180 |
| MS.gene029651.t1 | MTR_3g065100 | 51.282 | 156 | 75 | 1 | 1 | 155 | 1 | 156 | 2.16e-42 | 141 |
| MS.gene029651.t1 | MTR_3g466980 | 44.805 | 154 | 84 | 1 | 1 | 153 | 1 | 154 | 9.47e-40 | 134 |
| MS.gene029651.t1 | MTR_4g032620 | 40.909 | 154 | 89 | 2 | 1 | 152 | 1 | 154 | 2.97e-39 | 132 |
| MS.gene029651.t1 | MTR_4g063790 | 42.857 | 154 | 86 | 2 | 1 | 152 | 1 | 154 | 4.55e-36 | 122 |
| MS.gene029651.t1 | MTR_3g466830 | 42.857 | 154 | 87 | 1 | 3 | 155 | 2 | 155 | 6.72e-34 | 117 |
| MS.gene029651.t1 | MTR_8g036130 | 42.767 | 159 | 87 | 2 | 1 | 155 | 1 | 159 | 2.02e-33 | 118 |
| MS.gene029651.t1 | MTR_5g075380 | 35.897 | 156 | 99 | 1 | 1 | 155 | 1 | 156 | 1.70e-32 | 113 |
| MS.gene029651.t1 | MTR_4g031910 | 43.590 | 156 | 86 | 2 | 1 | 154 | 1 | 156 | 2.81e-32 | 115 |
| MS.gene029651.t1 | MTR_3g031240 | 37.580 | 157 | 96 | 2 | 1 | 155 | 1 | 157 | 8.50e-31 | 109 |
| MS.gene029651.t1 | MTR_3g467080 | 38.217 | 157 | 95 | 2 | 1 | 155 | 1 | 157 | 1.84e-30 | 108 |
| MS.gene029651.t1 | MTR_3g466890 | 39.610 | 154 | 92 | 1 | 3 | 155 | 2 | 155 | 7.45e-30 | 106 |
| MS.gene029651.t1 | MTR_4g032260 | 42.949 | 156 | 87 | 2 | 1 | 154 | 1 | 156 | 8.74e-29 | 103 |
| MS.gene029651.t1 | MTR_3g031100 | 37.342 | 158 | 95 | 3 | 1 | 155 | 1 | 157 | 1.09e-28 | 106 |
| MS.gene029651.t1 | MTR_2g035610 | 45.763 | 118 | 63 | 1 | 1 | 117 | 1 | 118 | 1.10e-28 | 102 |
| MS.gene029651.t1 | MTR_4g032290 | 44.231 | 156 | 85 | 2 | 1 | 154 | 1 | 156 | 4.35e-28 | 104 |
| MS.gene029651.t1 | MTR_2g016210 | 37.086 | 151 | 93 | 2 | 1 | 149 | 1 | 151 | 2.49e-26 | 100 |
| MS.gene029651.t1 | MTR_4g028800 | 53.425 | 73 | 32 | 1 | 1 | 73 | 1 | 71 | 3.04e-23 | 87.4 |
| MS.gene029651.t1 | MTR_2g035580 | 34.194 | 155 | 101 | 1 | 1 | 154 | 1 | 155 | 4.62e-23 | 89.4 |
| MS.gene029651.t1 | MTR_3g465410 | 38.889 | 126 | 75 | 2 | 29 | 152 | 1 | 126 | 5.24e-23 | 88.6 |
| MS.gene029651.t1 | MTR_4g019670 | 34.899 | 149 | 85 | 4 | 1 | 145 | 1 | 141 | 1.23e-18 | 82.0 |
| MS.gene029651.t1 | MTR_7g106510 | 32.639 | 144 | 88 | 3 | 3 | 145 | 6 | 141 | 9.28e-18 | 79.3 |
| MS.gene029651.t1 | MTR_3g466900 | 34.400 | 125 | 81 | 1 | 32 | 155 | 2 | 126 | 2.47e-17 | 73.9 |
| MS.gene029651.t1 | MTR_3g466930 | 34.400 | 125 | 81 | 1 | 32 | 155 | 2 | 126 | 2.93e-17 | 73.6 |
| MS.gene029651.t1 | MTR_1g033930 | 45.161 | 93 | 50 | 1 | 64 | 155 | 22 | 114 | 6.81e-17 | 72.4 |
| MS.gene029651.t1 | MTR_7g055800 | 35.043 | 117 | 49 | 2 | 1 | 117 | 1 | 90 | 2.26e-16 | 70.1 |
| MS.gene029651.t1 | MTR_7g055790 | 39.640 | 111 | 48 | 2 | 1 | 110 | 59 | 151 | 6.48e-16 | 70.5 |
| MS.gene029651.t1 | MTR_1g033370 | 43.000 | 100 | 56 | 1 | 57 | 155 | 21 | 120 | 6.66e-15 | 67.4 |
| MS.gene029651.t1 | MTR_1g033790 | 42.222 | 90 | 51 | 1 | 64 | 152 | 22 | 111 | 2.44e-13 | 63.2 |
| MS.gene029651.t1 | MTR_6g005450 | 31.481 | 108 | 68 | 3 | 1 | 102 | 1 | 108 | 1.88e-12 | 63.9 |
| MS.gene029651.t1 | MTR_7g055940 | 34.314 | 102 | 40 | 2 | 1 | 102 | 1 | 75 | 2.75e-12 | 59.3 |
| MS.gene029651.t1 | MTR_6g018920 | 26.875 | 160 | 97 | 6 | 1 | 154 | 1 | 146 | 1.96e-11 | 60.8 |
| MS.gene029651.t1 | MTR_3g093900 | 28.829 | 111 | 78 | 1 | 3 | 112 | 6 | 116 | 2.29e-11 | 61.2 |
| MS.gene029651.t1 | MTR_1g114730 | 28.440 | 109 | 70 | 3 | 1 | 102 | 1 | 108 | 2.87e-11 | 60.5 |
| MS.gene029651.t1 | MTR_5g066180 | 34.513 | 113 | 57 | 3 | 1 | 113 | 1 | 96 | 7.11e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029651.t1 | AT5G26630 | 46.154 | 156 | 83 | 1 | 1 | 155 | 1 | 156 | 5.52e-39 | 132 |
| MS.gene029651.t1 | AT5G48670 | 47.436 | 156 | 81 | 1 | 1 | 155 | 1 | 156 | 1.41e-36 | 128 |
| MS.gene029651.t1 | AT1G65330 | 42.857 | 154 | 87 | 1 | 3 | 155 | 2 | 155 | 2.78e-36 | 126 |
| MS.gene029651.t1 | AT1G65300 | 40.909 | 154 | 90 | 1 | 3 | 155 | 2 | 155 | 1.04e-34 | 122 |
| MS.gene029651.t1 | AT3G05860 | 41.611 | 149 | 86 | 1 | 1 | 148 | 1 | 149 | 1.79e-34 | 120 |
| MS.gene029651.t1 | AT3G05860 | 41.611 | 149 | 86 | 1 | 1 | 148 | 1 | 149 | 6.53e-34 | 119 |
| MS.gene029651.t1 | AT3G05860 | 41.611 | 149 | 86 | 1 | 1 | 148 | 1 | 149 | 1.14e-33 | 119 |
| MS.gene029651.t1 | AT5G26650 | 37.908 | 153 | 94 | 1 | 4 | 155 | 2 | 154 | 3.88e-30 | 112 |
| MS.gene029651.t1 | AT5G27960 | 36.601 | 153 | 96 | 1 | 4 | 155 | 2 | 154 | 4.42e-28 | 106 |
| MS.gene029651.t1 | AT1G31630 | 31.126 | 151 | 102 | 2 | 3 | 152 | 2 | 151 | 3.62e-25 | 99.0 |
| MS.gene029651.t1 | AT1G22590 | 33.333 | 156 | 100 | 3 | 1 | 155 | 1 | 153 | 8.89e-23 | 89.0 |
| MS.gene029651.t1 | AT1G31640 | 34.000 | 150 | 97 | 2 | 3 | 151 | 2 | 150 | 1.19e-22 | 93.2 |
| MS.gene029651.t1 | AT2G28700 | 38.017 | 121 | 68 | 2 | 1 | 117 | 1 | 118 | 3.04e-21 | 88.2 |
| MS.gene029651.t1 | AT5G27810 | 43.333 | 90 | 50 | 1 | 29 | 117 | 1 | 90 | 6.05e-21 | 82.8 |
| MS.gene029651.t1 | AT5G26580 | 34.194 | 155 | 89 | 1 | 1 | 155 | 1 | 142 | 1.75e-20 | 86.3 |
| MS.gene029651.t1 | AT2G40210 | 36.154 | 130 | 78 | 2 | 1 | 127 | 1 | 128 | 4.05e-16 | 74.3 |
| MS.gene029651.t1 | AT5G06500 | 34.247 | 146 | 83 | 5 | 1 | 141 | 1 | 138 | 2.69e-15 | 70.9 |
| MS.gene029651.t1 | AT5G58890 | 35.417 | 96 | 55 | 2 | 1 | 89 | 1 | 96 | 1.66e-11 | 60.8 |
| MS.gene029651.t1 | AT1G47760 | 33.333 | 111 | 67 | 3 | 1 | 110 | 1 | 105 | 2.38e-11 | 59.3 |
| MS.gene029651.t1 | AT5G55690 | 29.167 | 120 | 78 | 2 | 1 | 113 | 1 | 120 | 4.45e-11 | 59.7 |
| MS.gene029651.t1 | AT5G55690 | 29.167 | 120 | 78 | 2 | 1 | 113 | 1 | 120 | 4.45e-11 | 59.7 |
Find 25 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCTTTCATTGATGAGTTC+TGG | 0.252705 | 5.4:+37378037 | None:intergenic |
| CTCTAATGATTCGGCAAGAA+AGG | 0.394804 | 5.4:-37378217 | MS.gene029651:CDS |
| GAGCTGAACAATCTTATGAT+TGG | 0.404054 | 5.4:-37377919 | MS.gene029651:CDS |
| CTTTAGTAATCCTCTGCAAT+AGG | 0.413033 | 5.4:+37377986 | None:intergenic |
| GTACCTCTCAATTACCTGTC+TGG | 0.443679 | 5.4:+37378059 | None:intergenic |
| TGAGAAACAGATAATTCATC+TGG | 0.457651 | 5.4:+37377874 | None:intergenic |
| AGCTGAACAATCTTATGATT+GGG | 0.458403 | 5.4:-37377918 | MS.gene029651:CDS |
| AGAGAACTCACCATTCTTTG+TGG | 0.496533 | 5.4:-37378153 | MS.gene029651:CDS |
| GCTCCTTCTCACGATTATCA+TGG | 0.497017 | 5.4:+37377938 | None:intergenic |
| TGCTGGAATACCACAAAGAA+TGG | 0.514499 | 5.4:+37378143 | None:intergenic |
| TGAGAAAATTCTGAAGGACA+TGG | 0.544670 | 5.4:-37377827 | MS.gene029651:CDS |
| TTGGAAATTATAGCACATGC+TGG | 0.559487 | 5.4:+37378126 | None:intergenic |
| TCTGCAATAGGAAACTCTCT+TGG | 0.562811 | 5.4:+37377998 | None:intergenic |
| AGAGGTGTGGCCAGATCTAG+AGG | 0.572723 | 5.4:-37378085 | MS.gene029651:CDS |
| CAACGCCATGACTAGGAAGA+AGG | 0.575303 | 5.4:-37378256 | None:intergenic |
| GCTTATTGAGAAAATTCTGA+AGG | 0.584834 | 5.4:-37377833 | MS.gene029651:CDS |
| AAGAGAGTTTCCTATTGCAG+AGG | 0.589804 | 5.4:-37377996 | MS.gene029651:CDS |
| TTAATTCTAAAACAGAGGTG+TGG | 0.602716 | 5.4:-37378098 | MS.gene029651:CDS |
| TGCATCACAACGCCATGACT+AGG | 0.604632 | 5.4:-37378263 | None:intergenic |
| GATAACAAGATCGATGCACT+TGG | 0.609178 | 5.4:-37377805 | MS.gene029651:CDS |
| GAGGTGTGGCCAGATCTAGA+GGG | 0.615970 | 5.4:-37378084 | MS.gene029651:CDS |
| AAGCCATGATAATCGTGAGA+AGG | 0.628086 | 5.4:-37377941 | MS.gene029651:CDS |
| AGGTGTGGCCAGATCTAGAG+GGG | 0.656006 | 5.4:-37378083 | MS.gene029651:CDS |
| GGGCCAGACAGGTAATTGAG+AGG | 0.686598 | 5.4:-37378062 | MS.gene029651:CDS |
| GGTGTGGCCAGATCTAGAGG+GGG | 0.723497 | 5.4:-37378082 | MS.gene029651:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACCTACAATAAAAGAAAAA+AGG | - | chr5.4:37377857-37377876 | MS.gene029651:CDS | 20.0% |
| !! | ACCTACAATAAAAGAAAAAA+GGG | - | chr5.4:37377858-37377877 | MS.gene029651:CDS | 20.0% |
| !!! | TGTTTTAGAATTAAAAGGAT+TGG | + | chr5.4:37377946-37377965 | None:intergenic | 20.0% |
| !! | TGCTTTTATCTCTAATGATT+CGG | - | chr5.4:37377824-37377843 | MS.gene029651:CDS | 25.0% |
| !!! | ACCCTTTTTTCTTTTATTGT+AGG | + | chr5.4:37377862-37377881 | None:intergenic | 25.0% |
| !!! | ACCTCTGTTTTAGAATTAAA+AGG | + | chr5.4:37377951-37377970 | None:intergenic | 25.0% |
| !!! | TCCTTTTAATTCTAAAACAG+AGG | - | chr5.4:37377947-37377966 | MS.gene029651:CDS | 25.0% |
| AGCTGAACAATCTTATGATT+GGG | - | chr5.4:37378132-37378151 | MS.gene029651:CDS | 30.0% | |
| GCTTATTGAGAAAATTCTGA+AGG | - | chr5.4:37378217-37378236 | MS.gene029651:CDS | 30.0% | |
| TGAGAAACAGATAATTCATC+TGG | + | chr5.4:37378179-37378198 | None:intergenic | 30.0% | |
| !! | AAAAAAGGGTATCATCAAGA+AGG | - | chr5.4:37377872-37377891 | MS.gene029651:CDS | 30.0% |
| !! | TTAATTCTAAAACAGAGGTG+TGG | - | chr5.4:37377952-37377971 | MS.gene029651:CDS | 30.0% |
| CATCTTTCATTGATGAGTTC+TGG | + | chr5.4:37378016-37378035 | None:intergenic | 35.0% | |
| CTTTAGTAATCCTCTGCAAT+AGG | + | chr5.4:37378067-37378086 | None:intergenic | 35.0% | |
| GAGCTGAACAATCTTATGAT+TGG | - | chr5.4:37378131-37378150 | MS.gene029651:CDS | 35.0% | |
| TTGGAAATTATAGCACATGC+TGG | + | chr5.4:37377927-37377946 | None:intergenic | 35.0% | |
| ! | TGAGAAAATTCTGAAGGACA+TGG | - | chr5.4:37378223-37378242 | MS.gene029651:CDS | 35.0% |
| AAGAGAGTTTCCTATTGCAG+AGG | - | chr5.4:37378054-37378073 | MS.gene029651:CDS | 40.0% | |
| AAGCCATGATAATCGTGAGA+AGG | - | chr5.4:37378109-37378128 | MS.gene029651:CDS | 40.0% | |
| AGAGAACTCACCATTCTTTG+TGG | - | chr5.4:37377897-37377916 | MS.gene029651:CDS | 40.0% | |
| GATAACAAGATCGATGCACT+TGG | - | chr5.4:37378245-37378264 | MS.gene029651:CDS | 40.0% | |
| TCTGCAATAGGAAACTCTCT+TGG | + | chr5.4:37378055-37378074 | None:intergenic | 40.0% | |
| TGCTGGAATACCACAAAGAA+TGG | + | chr5.4:37377910-37377929 | None:intergenic | 40.0% | |
| ! | CTCTAATGATTCGGCAAGAA+AGG | - | chr5.4:37377833-37377852 | MS.gene029651:CDS | 40.0% |
| !!! | TTGCTTTTGAAGTTGCTCTC+TGG | + | chr5.4:37378090-37378109 | None:intergenic | 40.0% |
| GCTCCTTCTCACGATTATCA+TGG | + | chr5.4:37378115-37378134 | None:intergenic | 45.0% | |
| GTACCTCTCAATTACCTGTC+TGG | + | chr5.4:37377994-37378013 | None:intergenic | 45.0% | |
| AGGTGTGGCCAGATCTAGAG+GGG | - | chr5.4:37377967-37377986 | MS.gene029651:CDS | 55.0% | |
| GAGGTGTGGCCAGATCTAGA+GGG | - | chr5.4:37377966-37377985 | MS.gene029651:CDS | 55.0% | |
| GGGCCAGACAGGTAATTGAG+AGG | - | chr5.4:37377988-37378007 | MS.gene029651:CDS | 55.0% | |
| ! | AGAGGTGTGGCCAGATCTAG+AGG | - | chr5.4:37377965-37377984 | MS.gene029651:CDS | 55.0% |
| AGATCTAGAGGGGGCCAGAC+AGG | - | chr5.4:37377977-37377996 | MS.gene029651:CDS | 60.0% | |
| GGTGTGGCCAGATCTAGAGG+GGG | - | chr5.4:37377968-37377987 | MS.gene029651:CDS | 60.0% | |
| TGTCTGGCCCCCTCTAGATC+TGG | + | chr5.4:37377978-37377997 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 37377801 | 37378271 | 37377801 | ID=MS.gene029651 |
| chr5.4 | mRNA | 37377801 | 37378271 | 37377801 | ID=MS.gene029651.t1;Parent=MS.gene029651 |
| chr5.4 | exon | 37377801 | 37378271 | 37377801 | ID=MS.gene029651.t1.exon1;Parent=MS.gene029651.t1 |
| chr5.4 | CDS | 37377801 | 37378271 | 37377801 | ID=cds.MS.gene029651.t1;Parent=MS.gene029651.t1 |
| Gene Sequence |
| Protein sequence |
