Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029669.t1 | XP_003614339.1 | 98.3 | 300 | 5 | 0 | 1 | 300 | 1 | 300 | 1.40E-160 | 575.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029669.t1 | Q9CAA4 | 53.9 | 306 | 122 | 3 | 1 | 305 | 1 | 288 | 1.8e-81 | 304.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029669.t1 | G7JZB3 | 98.3 | 300 | 5 | 0 | 1 | 300 | 1 | 300 | 9.7e-161 | 575.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene029669.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029669.t1 | MTR_5g048350 | 98.333 | 300 | 5 | 0 | 1 | 300 | 1 | 300 | 0.0 | 613 |
| MS.gene029669.t1 | MTR_5g048350 | 95.333 | 300 | 5 | 1 | 1 | 300 | 1 | 291 | 0.0 | 587 |
| MS.gene029669.t1 | MTR_4g110040 | 51.825 | 274 | 111 | 8 | 35 | 292 | 124 | 392 | 1.52e-78 | 248 |
| MS.gene029669.t1 | MTR_4g110040 | 51.825 | 274 | 111 | 8 | 35 | 292 | 256 | 524 | 5.38e-77 | 247 |
| MS.gene029669.t1 | MTR_7g017320 | 43.609 | 266 | 119 | 7 | 41 | 288 | 47 | 299 | 8.26e-54 | 181 |
| MS.gene029669.t1 | MTR_7g017320 | 46.821 | 173 | 79 | 3 | 41 | 213 | 47 | 206 | 2.53e-41 | 146 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029669.t1 | AT1G69010 | 56.014 | 291 | 109 | 3 | 1 | 290 | 1 | 273 | 4.64e-111 | 327 |
| MS.gene029669.t1 | AT5G08130 | 40.081 | 247 | 113 | 6 | 46 | 288 | 158 | 373 | 3.58e-48 | 168 |
| MS.gene029669.t1 | AT5G08130 | 39.676 | 247 | 115 | 6 | 46 | 288 | 158 | 374 | 2.49e-47 | 166 |
| MS.gene029669.t1 | AT5G08130 | 39.759 | 249 | 121 | 5 | 41 | 288 | 273 | 493 | 8.85e-47 | 167 |
| MS.gene029669.t1 | AT5G08130 | 39.759 | 249 | 121 | 5 | 41 | 288 | 277 | 497 | 9.52e-47 | 167 |
| MS.gene029669.t1 | AT5G08130 | 39.357 | 249 | 123 | 5 | 41 | 288 | 273 | 494 | 5.96e-46 | 164 |
| MS.gene029669.t1 | AT5G08130 | 39.357 | 249 | 123 | 5 | 41 | 288 | 273 | 494 | 5.96e-46 | 164 |
| MS.gene029669.t1 | AT5G08130 | 39.357 | 249 | 123 | 5 | 41 | 288 | 277 | 498 | 6.20e-46 | 164 |
| MS.gene029669.t1 | AT5G08130 | 39.357 | 249 | 123 | 5 | 41 | 288 | 273 | 494 | 6.21e-46 | 164 |
| MS.gene029669.t1 | AT5G38860 | 37.793 | 299 | 142 | 7 | 27 | 320 | 16 | 275 | 2.70e-45 | 157 |
| MS.gene029669.t1 | AT5G38860 | 45.963 | 161 | 75 | 2 | 27 | 185 | 16 | 166 | 2.05e-35 | 129 |
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCGTGATCTATCATATTA+TGG | 0.225408 | 5.4:+37672928 | None:intergenic |
| GGTTGAAATCTTTGCTTATA+AGG | 0.241879 | 5.4:-37666541 | MS.gene029669:CDS |
| GAGGTTGACCGACAAAATTT+TGG | 0.267640 | 5.4:+37673045 | None:intergenic |
| TGTACTTTCCCATCCTCTTC+AGG | 0.272008 | 5.4:-37672790 | MS.gene029669:CDS |
| GTATGAGATCCCTCAATATT+TGG | 0.276214 | 5.4:+37674050 | None:intergenic |
| AGGGTACCTTTGTTGGTGTT+AGG | 0.276576 | 5.4:+37675890 | None:intergenic |
| GGGTACCTTTGTTGGTGTTA+GGG | 0.323081 | 5.4:+37675891 | None:intergenic |
| GGCGGAGAGGCAACCTGATT+TGG | 0.323743 | 5.4:-37672880 | MS.gene029669:CDS |
| CACAGCTTCGTTCTTATTAG+AGG | 0.328656 | 5.4:-37674005 | MS.gene029669:intron |
| ATTTCAGTTCAAATTGATCT+TGG | 0.329165 | 5.4:-37670043 | MS.gene029669:CDS |
| CCTGAACCATTCTTTACAAT+TGG | 0.334279 | 5.4:+37673023 | None:intergenic |
| TTTCCGGGAAACGGTGATAC+TGG | 0.349733 | 5.4:+37672999 | None:intergenic |
| GTTGAAATCTTTGCTTATAA+GGG | 0.359473 | 5.4:-37666540 | MS.gene029669:CDS |
| ATTCCCTTGCTCGCCAAATC+AGG | 0.384265 | 5.4:+37672867 | None:intergenic |
| TTAAGGAAGGGTACCTTTGT+TGG | 0.391183 | 5.4:+37675883 | None:intergenic |
| TGAGTATGTTCAGTACTTAC+AGG | 0.412725 | 5.4:-37673881 | MS.gene029669:CDS |
| TGGGAGCCACTAAGCATTGT+TGG | 0.420920 | 5.4:+37672948 | None:intergenic |
| ATCTCTCCAACAATGCTTAG+TGG | 0.434167 | 5.4:-37672954 | MS.gene029669:CDS |
| ATCGTGATCTATCATATTAT+GGG | 0.435013 | 5.4:+37672929 | None:intergenic |
| GTACTTTCCCATCCTCTTCA+GGG | 0.437126 | 5.4:-37672789 | MS.gene029669:CDS |
| TTTCAGTTCAAATTGATCTT+GGG | 0.437219 | 5.4:-37670042 | MS.gene029669:CDS |
| CCCTGAGTTAAAATATGAAC+GGG | 0.439771 | 5.4:-37666562 | MS.gene029669:CDS |
| CCAATTGTAAAGAATGGTTC+AGG | 0.442919 | 5.4:-37673023 | MS.gene029669:CDS |
| CCTTCAGATTCCAAATATTG+AGG | 0.448909 | 5.4:-37674060 | MS.gene029669:intron |
| CTTCAGATTCCAAATATTGA+GGG | 0.451155 | 5.4:-37674059 | MS.gene029669:intron |
| CAACTGTAAGTTCGTCCTGC+TGG | 0.451238 | 5.4:+37672712 | None:intergenic |
| TCATCAAACTTTCCGGGAAA+CGG | 0.451349 | 5.4:+37672990 | None:intergenic |
| GAACCATTCTTTACAATTGG+AGG | 0.459728 | 5.4:+37673026 | None:intergenic |
| GGAGATGAGGCTGTGCCACT+TGG | 0.463787 | 5.4:+37670001 | None:intergenic |
| ACTCAAGCGCTACAGAGTGC+TGG | 0.473626 | 5.4:-37670088 | MS.gene029669:CDS |
| GGTCCAGTATCACCGTTTCC+CGG | 0.476824 | 5.4:-37673002 | MS.gene029669:CDS |
| CAACCTCCAATTGTAAAGAA+TGG | 0.480239 | 5.4:-37673029 | MS.gene029669:CDS |
| TTTGTTGGTGTTAGGGGCAG+AGG | 0.484917 | 5.4:+37675898 | None:intergenic |
| CAGGACGAACTTACAGTTGA+AGG | 0.485742 | 5.4:-37672708 | MS.gene029669:CDS |
| AAATATGAAGGCTCGTATCA+AGG | 0.490493 | 5.4:-37673847 | MS.gene029669:CDS |
| GCAACCTGATTTGGCGAGCA+AGG | 0.490959 | 5.4:-37672871 | MS.gene029669:CDS |
| TGATAGATCACGATCCGACT+CGG | 0.499986 | 5.4:-37672920 | MS.gene029669:CDS |
| GTCTTTGACAAAGTTACCAT+TGG | 0.503667 | 5.4:+37666584 | None:intergenic |
| CAAGGGAATTCCTCTGCCTA+TGG | 0.504233 | 5.4:-37672853 | MS.gene029669:CDS |
| TCTTGGGAAGCGTGCAAAGA+AGG | 0.509091 | 5.4:-37670026 | MS.gene029669:CDS |
| TTGAGTTGTTCACTCGTTGT+GGG | 0.512133 | 5.4:+37672735 | None:intergenic |
| TTGCTTATGTTAACATGTGA+TGG | 0.515374 | 5.4:-37666446 | None:intergenic |
| ATGAAGGCTCGTATCAAGGC+TGG | 0.517535 | 5.4:-37673843 | MS.gene029669:CDS |
| CTCAAGCGCTACAGAGTGCT+GGG | 0.520453 | 5.4:-37670087 | MS.gene029669:CDS |
| TACCTCAATGTGATCAAAAG+AGG | 0.521201 | 5.4:-37674030 | MS.gene029669:CDS |
| GGTACCTTTGTTGGTGTTAG+GGG | 0.522122 | 5.4:+37675892 | None:intergenic |
| TTGCCTACAACAATGAAAGC+AGG | 0.539094 | 5.4:-37675989 | None:intergenic |
| GATAGATCACGATCCGACTC+GGG | 0.540435 | 5.4:-37672919 | MS.gene029669:CDS |
| AGATGGGAAAGCTACTGATA+AGG | 0.542435 | 5.4:-37674821 | MS.gene029669:CDS |
| AAACAGTTCCCTGAAGAGGA+TGG | 0.542486 | 5.4:+37672781 | None:intergenic |
| GTTGAGTTGTTCACTCGTTG+TGG | 0.544799 | 5.4:+37672734 | None:intergenic |
| AACCGTCGAAGTTGATGCCA+TGG | 0.544835 | 5.4:-37673813 | MS.gene029669:CDS |
| TCTGTTCCTGTCAGAAGTGA+TGG | 0.550958 | 5.4:-37672813 | MS.gene029669:CDS |
| ACCCATGGCATCAACTTCGA+CGG | 0.559522 | 5.4:+37673811 | None:intergenic |
| TCTGTATACCTTGGGAGATG+AGG | 0.560824 | 5.4:+37669988 | None:intergenic |
| AACAGTTCCCTGAAGAGGAT+GGG | 0.561389 | 5.4:+37672782 | None:intergenic |
| ATCTCGAGTGTATACTCCCA+AGG | 0.571871 | 5.4:-37672672 | MS.gene029669:CDS |
| ACCCTGAGTTAAAATATGAA+CGG | 0.577331 | 5.4:-37666563 | MS.gene029669:CDS |
| GACTATGATGTTGTTTACTG+CGG | 0.582288 | 5.4:-37666616 | MS.gene029669:CDS |
| ACTGCGGTGTTCAATACCAA+TGG | 0.583759 | 5.4:-37666600 | MS.gene029669:CDS |
| ACATTCAGTTGATTGTGCAT+CGG | 0.585244 | 5.4:+37672758 | None:intergenic |
| ATGTTTGCATGCATAGCCAT+AGG | 0.589737 | 5.4:+37672837 | None:intergenic |
| GATCCGACTCGGGATATTGT+CGG | 0.590573 | 5.4:-37672909 | MS.gene029669:CDS |
| CAACCTGATTTGGCGAGCAA+GGG | 0.591521 | 5.4:-37672870 | MS.gene029669:CDS |
| GAAGAAGCAAGATAAATGAG+AGG | 0.595996 | 5.4:-37674756 | MS.gene029669:intron |
| TCGGAAACAGTTCCCTGAAG+AGG | 0.597102 | 5.4:+37672777 | None:intergenic |
| TGCCTACAACAATGAAAGCA+GGG | 0.600473 | 5.4:-37675988 | None:intergenic |
| GAGTGAACAACTCAACCAGC+AGG | 0.605106 | 5.4:-37672727 | MS.gene029669:CDS |
| GCATGCATAGCCATAGGCAG+AGG | 0.609045 | 5.4:+37672843 | None:intergenic |
| ACCTCAATGTGATCAAAAGA+GGG | 0.612540 | 5.4:-37674029 | MS.gene029669:CDS |
| AGTACACCATCACTTCTGAC+AGG | 0.614723 | 5.4:+37672807 | None:intergenic |
| TCGGGATATTGTCGGCAAGA+CGG | 0.618174 | 5.4:-37672901 | MS.gene029669:CDS |
| AACATTCTGTAACGGAGCAG+CGG | 0.620536 | 5.4:-37674780 | MS.gene029669:CDS |
| TGGCACAGCCTCATCTCCCA+AGG | 0.623367 | 5.4:-37669996 | MS.gene029669:intron |
| ACCGTCGAAGTTGATGCCAT+GGG | 0.624180 | 5.4:-37673812 | MS.gene029669:intron |
| AAGATCGAAACATTCTGTAA+CGG | 0.625212 | 5.4:-37674788 | MS.gene029669:CDS |
| GACGAACTTACAGTTGAAGG+AGG | 0.634609 | 5.4:-37672705 | MS.gene029669:CDS |
| ATGTTCAGTACTTACAGGAG+AGG | 0.638295 | 5.4:-37673876 | MS.gene029669:CDS |
| TGCTGGGTTAGATCTGTCAA+AGG | 0.641347 | 5.4:-37670071 | MS.gene029669:CDS |
| TGTTCAGTACTTACAGGAGA+GGG | 0.653015 | 5.4:-37673875 | MS.gene029669:CDS |
| TCTCGAGTGTATACTCCCAA+GGG | 0.653032 | 5.4:-37672671 | MS.gene029669:intron |
| GGATATTGTCGGCAAGACGG+CGG | 0.664149 | 5.4:-37672898 | MS.gene029669:CDS |
| TTGCCGACAATATCCCGAGT+CGG | 0.672673 | 5.4:+37672906 | None:intergenic |
| ACGAACTTACAGTTGAAGGA+GGG | 0.673949 | 5.4:-37672704 | MS.gene029669:CDS |
| TTGTCGGCAAGACGGCGGAG+AGG | 0.684251 | 5.4:-37672893 | MS.gene029669:CDS |
| TCTGCCCCTAACACCAACAA+AGG | 0.684939 | 5.4:-37675896 | MS.gene029669:intron |
| CGTGCAAAGAAGGATCCAAG+TGG | 0.732676 | 5.4:-37670016 | MS.gene029669:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 37666449 | 37675999 | 37666449 | ID=MS.gene029669 |
| chr5.4 | mRNA | 37666449 | 37675999 | 37666449 | ID=MS.gene029669.t1;Parent=MS.gene029669 |
| chr5.4 | exon | 37675897 | 37675999 | 37675897 | ID=MS.gene029669.t1.exon1;Parent=MS.gene029669.t1 |
| chr5.4 | CDS | 37675897 | 37675999 | 37675897 | ID=cds.MS.gene029669.t1;Parent=MS.gene029669.t1 |
| chr5.4 | exon | 37674757 | 37674841 | 37674757 | ID=MS.gene029669.t1.exon2;Parent=MS.gene029669.t1 |
| chr5.4 | CDS | 37674757 | 37674841 | 37674757 | ID=cds.MS.gene029669.t1;Parent=MS.gene029669.t1 |
| chr5.4 | exon | 37674006 | 37674075 | 37674006 | ID=MS.gene029669.t1.exon3;Parent=MS.gene029669.t1 |
| chr5.4 | CDS | 37674006 | 37674075 | 37674006 | ID=cds.MS.gene029669.t1;Parent=MS.gene029669.t1 |
| chr5.4 | exon | 37673813 | 37673908 | 37673813 | ID=MS.gene029669.t1.exon4;Parent=MS.gene029669.t1 |
| chr5.4 | CDS | 37673813 | 37673908 | 37673813 | ID=cds.MS.gene029669.t1;Parent=MS.gene029669.t1 |
| chr5.4 | exon | 37672672 | 37673087 | 37672672 | ID=MS.gene029669.t1.exon5;Parent=MS.gene029669.t1 |
| chr5.4 | CDS | 37672672 | 37673087 | 37672672 | ID=cds.MS.gene029669.t1;Parent=MS.gene029669.t1 |
| chr5.4 | exon | 37669997 | 37670126 | 37669997 | ID=MS.gene029669.t1.exon6;Parent=MS.gene029669.t1 |
| chr5.4 | CDS | 37669997 | 37670126 | 37669997 | ID=cds.MS.gene029669.t1;Parent=MS.gene029669.t1 |
| chr5.4 | exon | 37666449 | 37666637 | 37666449 | ID=MS.gene029669.t1.exon7;Parent=MS.gene029669.t1 |
| chr5.4 | CDS | 37666449 | 37666637 | 37666449 | ID=cds.MS.gene029669.t1;Parent=MS.gene029669.t1 |
| Gene Sequence |
| Protein sequence |
