AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene029717

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029717.t1 MTR_5g021670 70.968 186 20 1 41 192 242 427 2.01e-73 243
MS.gene029717.t1 MTR_5g021670 94.253 87 5 0 191 277 1007 1093 1.16e-37 141
MS.gene029717.t1 MTR_5g021670 30.380 158 90 3 40 197 511 648 1.47e-12 68.2
MS.gene029717.t1 MTR_5g021670 28.205 156 92 3 40 195 559 694 1.49e-11 65.1
MS.gene029717.t1 MTR_3g113140 76.744 86 20 0 193 278 1027 1112 2.41e-37 140
MS.gene029717.t1 MTR_3g113140 32.870 216 105 4 42 217 249 464 1.18e-22 98.2
MS.gene029717.t1 MTR_3g113140 27.536 207 98 5 33 195 509 707 2.35e-12 67.4
MS.gene029717.t1 MTR_5g026740 92.857 42 2 1 1 41 3 44 2.13e-19 81.3
MS.gene029717.t1 MTR_7g079550 29.609 179 103 5 40 203 321 491 1.72e-16 79.7
MS.gene029717.t1 MTR_7g009940 33.548 155 99 1 40 190 193 347 4.29e-15 75.5
MS.gene029717.t1 MTR_8g068540 33.121 157 84 3 40 195 455 591 2.76e-14 73.2
MS.gene029717.t1 MTR_2g016200 31.481 162 90 6 28 188 428 569 3.78e-13 69.7
MS.gene029717.t1 MTR_5g011410 29.193 161 98 3 42 192 206 360 4.27e-13 69.7
MS.gene029717.t1 MTR_7g081780 30.000 200 119 5 33 216 305 499 5.01e-13 69.7
MS.gene029717.t1 MTR_1g096270 32.704 159 84 5 40 197 102 238 5.78e-13 69.3
MS.gene029717.t1 MTR_3g048470 31.788 151 85 3 41 188 311 446 5.98e-13 68.9
MS.gene029717.t1 MTR_5g024450 30.818 159 89 3 42 199 467 605 1.30e-12 68.2
MS.gene029717.t1 MTR_4g105520 30.818 159 91 3 41 199 276 415 1.52e-12 68.2
MS.gene029717.t1 MTR_3g452850 28.655 171 102 4 40 199 245 406 1.59e-12 67.8
MS.gene029717.t1 MTR_5g096530 30.872 149 83 2 42 190 402 530 1.61e-12 67.8
MS.gene029717.t1 MTR_5g014720 29.714 175 95 4 34 192 264 426 1.64e-12 67.8
MS.gene029717.t1 MTR_3g452750 29.762 168 101 5 40 197 455 615 2.18e-12 67.4
MS.gene029717.t1 MTR_8g077105 30.247 162 92 6 28 188 296 437 2.26e-12 67.4
MS.gene029717.t1 MTR_3g109820 29.814 161 99 4 41 192 326 481 2.28e-12 67.4
MS.gene029717.t1 MTR_7g073290 31.481 162 93 4 37 197 301 445 2.39e-12 67.4
MS.gene029717.t1 MTR_7g073290 29.348 184 101 4 40 223 380 534 9.17e-12 65.9
MS.gene029717.t1 MTR_3g009400 29.197 137 77 2 52 188 397 513 2.49e-12 67.4
MS.gene029717.t1 MTR_7g081410 31.098 164 93 5 41 192 198 353 2.98e-12 67.0
MS.gene029717.t1 MTR_7g081410 31.098 164 93 5 41 192 318 473 4.11e-12 66.6
MS.gene029717.t1 MTR_2g014560 28.926 242 139 10 40 254 144 379 4.31e-12 66.6
MS.gene029717.t1 MTR_2g070020 26.238 202 111 4 42 210 397 593 4.71e-12 66.6
MS.gene029717.t1 MTR_2g070020 31.293 147 81 2 42 188 157 283 4.73e-11 63.5
MS.gene029717.t1 MTR_7g066620 26.923 234 142 8 40 249 55 283 4.75e-12 66.6
MS.gene029717.t1 MTR_3g090480 30.921 152 85 2 42 193 394 525 5.43e-12 66.2
MS.gene029717.t1 MTR_2g010470 32.278 158 93 4 40 188 404 556 6.45e-12 66.2
MS.gene029717.t1 MTR_4g070950 29.936 157 93 3 42 188 417 566 6.69e-12 65.9
MS.gene029717.t1 MTR_1g015530 34.459 148 74 6 42 188 121 246 7.64e-12 65.9
MS.gene029717.t1 MTR_1g015530 29.268 164 102 4 41 195 239 397 4.18e-11 63.5
MS.gene029717.t1 MTR_1g079520 30.000 180 106 3 42 221 361 520 8.19e-12 65.9
MS.gene029717.t1 MTR_1g015530 34.459 148 74 6 42 188 150 275 9.24e-12 65.9
MS.gene029717.t1 MTR_1g015530 29.268 164 102 4 41 195 268 426 5.29e-11 63.5
MS.gene029717.t1 MTR_7g091680 27.273 176 111 4 42 208 192 359 9.35e-12 65.5
MS.gene029717.t1 MTR_7g007820 32.450 151 79 3 43 192 207 335 1.16e-11 65.5
MS.gene029717.t1 MTR_6g036790 28.571 154 91 2 42 195 437 571 1.27e-11 65.5
MS.gene029717.t1 MTR_4g070970 30.573 157 92 4 42 188 383 532 1.30e-11 65.1
MS.gene029717.t1 MTR_3g060880 30.323 155 88 2 42 196 407 541 1.55e-11 65.1
MS.gene029717.t1 MTR_3g060880 29.299 157 88 4 40 195 501 635 4.59e-11 63.5
MS.gene029717.t1 MTR_4g028090 27.174 184 105 4 40 223 297 451 1.72e-11 64.7
MS.gene029717.t1 MTR_1g090520 33.333 156 84 5 40 195 98 233 2.02e-11 64.7
MS.gene029717.t1 MTR_2g029010 23.077 182 108 3 41 192 328 507 2.38e-11 64.3
MS.gene029717.t1 MTR_2g040910 28.571 154 91 2 42 195 462 596 2.54e-11 64.3
MS.gene029717.t1 MTR_6g036840 29.878 164 100 2 42 195 438 596 2.66e-11 64.3
MS.gene029717.t1 MTR_5g086570 31.126 151 78 4 42 192 615 739 2.69e-11 64.3
MS.gene029717.t1 MTR_8g041190 27.711 166 104 2 41 200 568 723 2.70e-11 64.3
MS.gene029717.t1 MTR_8g040925 27.711 166 104 2 41 200 568 723 2.70e-11 64.3
MS.gene029717.t1 MTR_5g026000 28.743 167 106 4 38 195 422 584 2.75e-11 64.3
MS.gene029717.t1 MTR_5g083480 31.973 147 85 2 42 188 225 356 3.19e-11 63.9
MS.gene029717.t1 MTR_5g083480 31.973 147 85 2 42 188 222 353 3.37e-11 63.9
MS.gene029717.t1 MTR_1g069470 30.189 159 95 3 40 188 292 444 3.85e-11 63.9
MS.gene029717.t1 MTR_3g092360 31.724 145 83 3 58 188 45 187 3.89e-11 63.2
MS.gene029717.t1 MTR_7g078340 31.148 183 104 5 25 205 378 540 4.01e-11 63.9
MS.gene029717.t1 MTR_3g449540 27.368 190 104 4 42 212 334 508 4.10e-11 63.5
MS.gene029717.t1 MTR_3g449540 30.282 142 80 4 41 182 133 255 4.62e-11 63.5
MS.gene029717.t1 MTR_4g017710 32.515 163 84 7 47 193 536 688 4.47e-11 63.5
MS.gene029717.t1 MTR_6g036870 29.878 164 100 2 42 195 401 559 4.59e-11 63.5
MS.gene029717.t1 MTR_3g093930 29.213 178 95 6 33 192 182 346 4.87e-11 63.5
MS.gene029717.t1 MTR_3g093930 29.032 155 91 2 42 196 119 254 5.80e-11 63.2
MS.gene029717.t1 MTR_1g102500 29.878 164 88 5 40 188 141 292 5.15e-11 63.5
MS.gene029717.t1 MTR_4g014350 29.878 164 94 3 26 188 63 206 5.24e-11 63.2
MS.gene029717.t1 MTR_0087s0050 27.848 158 93 3 27 184 573 709 5.24e-11 63.5
MS.gene029717.t1 MTR_1g040545 30.675 163 97 5 40 192 273 429 5.41e-11 63.5
MS.gene029717.t1 MTR_4g094790 31.579 152 98 3 46 192 360 510 5.86e-11 63.2
MS.gene029717.t1 MTR_4g019010 32.727 165 95 5 38 191 589 748 6.07e-11 63.2
MS.gene029717.t1 MTR_4g040420 30.682 176 103 7 29 192 525 693 6.91e-11 63.2
MS.gene029717.t1 MTR_4g130390 30.464 151 78 3 40 186 386 513 7.09e-11 63.2
MS.gene029717.t1 MTR_4g016800 31.169 154 82 4 40 192 388 518 7.53e-11 62.8
MS.gene029717.t1 MTR_2g016530 30.464 151 86 4 42 192 433 564 7.73e-11 62.8
MS.gene029717.t1 MTR_4g035200 28.571 147 85 2 42 188 156 282 8.26e-11 62.4
MS.gene029717.t1 MTR_4g016850 29.936 157 89 2 40 196 536 671 9.56e-11 62.8
MS.gene029717.t1 MTR_2g017470 29.730 148 87 4 40 176 600 741 9.87e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029717.t1 AT4G36180 86.207 87 12 0 191 277 1012 1098 3.25e-35 134
MS.gene029717.t1 AT4G36180 43.617 188 66 4 40 192 242 424 1.47e-33 129
MS.gene029717.t1 AT4G36180 32.667 150 81 3 42 191 366 495 1.71e-13 70.9
MS.gene029717.t1 AT4G36180 28.105 153 86 3 42 192 438 568 5.91e-11 63.5
MS.gene029717.t1 AT1G75640 84.146 82 13 0 193 274 1013 1094 4.06e-31 122
MS.gene029717.t1 AT1G75640 35.638 188 86 3 40 192 243 430 5.64e-28 113
MS.gene029717.t1 AT1G75640 28.302 159 94 2 42 200 372 510 2.69e-11 64.3
MS.gene029717.t1 AT1G73066 32.642 193 113 5 28 209 310 496 3.94e-17 81.6
MS.gene029717.t1 AT1G73066 32.099 162 94 4 41 192 154 309 1.01e-11 65.5
MS.gene029717.t1 AT1G65380 31.875 160 95 3 40 190 323 477 2.71e-15 76.3
MS.gene029717.t1 AT5G46330 32.468 154 84 2 42 195 465 598 5.60e-15 75.5
MS.gene029717.t1 AT5G46330 32.468 154 84 2 42 195 465 598 5.60e-15 75.5
MS.gene029717.t1 AT3G24240 30.120 166 102 4 41 197 403 563 6.06e-15 75.5
MS.gene029717.t1 AT4G26540 31.667 180 100 6 41 212 463 627 1.65e-14 73.9
MS.gene029717.t1 AT5G07280 30.435 161 86 4 41 192 529 672 5.88e-14 72.4
MS.gene029717.t1 AT5G63930 31.646 158 94 5 42 190 395 547 1.30e-13 71.2
MS.gene029717.t1 AT5G63930 28.814 177 105 3 29 205 431 586 1.42e-12 68.2
MS.gene029717.t1 AT5G63930 31.646 158 94 5 42 190 331 483 1.39e-13 71.2
MS.gene029717.t1 AT5G63930 28.814 177 105 3 29 205 367 522 1.48e-12 68.2
MS.gene029717.t1 AT1G08590 32.877 146 78 4 43 188 88 213 1.50e-13 71.2
MS.gene029717.t1 AT1G08590 30.612 147 82 2 42 188 327 453 4.41e-11 63.5
MS.gene029717.t1 AT2G34930 29.825 171 103 5 37 195 691 856 1.92e-13 70.9
MS.gene029717.t1 AT1G73080 31.579 190 113 6 28 206 312 495 2.38e-13 70.5
MS.gene029717.t1 AT4G04220 29.412 170 101 4 42 195 547 713 2.48e-13 70.5
MS.gene029717.t1 AT5G56040 30.539 167 95 4 41 195 466 623 2.59e-13 70.5
MS.gene029717.t1 AT5G56040 30.539 167 95 4 41 195 466 623 4.10e-13 69.7
MS.gene029717.t1 AT1G17240 32.919 161 91 4 41 190 283 437 4.58e-13 69.7
MS.gene029717.t1 AT1G17240 32.919 161 91 4 41 190 278 432 5.40e-13 69.3
MS.gene029717.t1 AT3G47570 29.630 189 112 4 42 229 395 563 1.01e-12 68.6
MS.gene029717.t1 AT5G44700 30.968 155 99 3 44 192 349 501 1.04e-12 68.6
MS.gene029717.t1 AT4G28650 31.973 147 80 2 42 188 318 444 1.09e-12 68.6
MS.gene029717.t1 AT2G25790 33.553 152 76 4 40 188 80 209 1.35e-12 68.2
MS.gene029717.t1 AT2G25790 29.448 163 98 4 42 195 419 573 3.48e-11 63.9
MS.gene029717.t1 AT5G27060 31.677 161 88 5 40 198 153 293 1.90e-12 67.8
MS.gene029717.t1 AT5G27060 31.677 161 88 5 40 198 153 293 1.90e-12 67.8
MS.gene029717.t1 AT5G27060 31.677 161 88 5 40 198 154 294 1.96e-12 67.8
MS.gene029717.t1 AT3G28040 29.775 178 104 6 28 192 218 387 1.98e-12 67.8
MS.gene029717.t1 AT5G48940 27.273 165 106 2 42 197 405 564 3.33e-12 67.0
MS.gene029717.t1 AT2G33170 32.484 157 85 5 40 195 549 685 3.42e-12 67.0
MS.gene029717.t1 AT2G33170 32.484 157 85 5 40 195 549 685 3.42e-12 67.0
MS.gene029717.t1 AT1G17750 28.342 187 119 4 28 205 289 469 4.12e-12 67.0
MS.gene029717.t1 AT1G07390 28.481 158 90 4 28 184 610 745 5.65e-12 66.2
MS.gene029717.t1 AT1G07390 28.481 158 90 4 28 184 639 774 6.00e-12 66.2
MS.gene029717.t1 AT1G07390 29.747 158 88 5 28 184 645 780 7.22e-12 66.2
MS.gene029717.t1 AT1G07390 28.481 158 90 4 28 184 596 731 8.29e-12 65.9
MS.gene029717.t1 AT1G07390 29.114 158 89 5 28 184 475 610 8.75e-12 65.9
MS.gene029717.t1 AT1G74190 29.282 181 111 5 40 211 543 715 9.81e-12 65.9
MS.gene029717.t1 AT1G71400 31.282 195 102 6 42 207 264 455 1.01e-11 65.5
MS.gene029717.t1 AT1G31812 69.048 42 12 1 1 41 3 44 1.62e-11 60.1
MS.gene029717.t1 AT2G15080 29.189 185 116 5 19 192 120 300 1.76e-11 65.1
MS.gene029717.t1 AT2G15080 29.189 185 116 5 19 192 120 300 1.76e-11 65.1
MS.gene029717.t1 AT5G66330 29.630 162 89 3 42 195 231 375 1.92e-11 64.3
MS.gene029717.t1 AT1G17230 32.653 147 79 2 42 188 149 275 1.97e-11 64.7
MS.gene029717.t1 AT1G17230 27.391 230 140 6 26 246 398 609 3.68e-11 63.9
MS.gene029717.t1 AT5G23400 28.750 160 106 4 42 195 142 299 2.16e-11 64.3
MS.gene029717.t1 AT1G74360 32.051 156 89 5 50 195 151 299 2.17e-11 64.7
MS.gene029717.t1 AT1G74360 27.273 187 97 4 40 192 260 441 2.21e-11 64.7
MS.gene029717.t1 AT2G42800 31.694 183 110 6 40 213 221 397 2.31e-11 64.3
MS.gene029717.t1 AT1G17230 32.653 147 79 2 42 188 149 275 2.45e-11 64.7
MS.gene029717.t1 AT1G17230 27.391 230 140 6 26 246 398 609 4.33e-11 63.9
MS.gene029717.t1 AT1G17230 32.653 147 79 2 42 188 149 275 2.80e-11 64.3
MS.gene029717.t1 AT1G17230 27.391 230 140 6 26 246 398 609 3.49e-11 63.9
MS.gene029717.t1 AT3G53590 32.051 156 91 4 42 188 192 341 3.00e-11 64.3
MS.gene029717.t1 AT5G62230 29.878 164 88 5 40 188 127 278 3.09e-11 64.3
MS.gene029717.t1 AT5G07180 29.143 175 104 3 42 216 250 404 3.53e-11 63.9
MS.gene029717.t1 AT1G28440 31.361 169 87 6 40 192 285 440 3.78e-11 63.9
MS.gene029717.t1 AT5G62230 29.878 164 88 5 40 188 127 278 3.79e-11 63.9
MS.gene029717.t1 AT3G56370 27.228 202 116 5 40 228 175 358 4.34e-11 63.5
MS.gene029717.t1 AT4G08850 28.571 154 90 3 40 193 342 475 4.55e-11 63.5
MS.gene029717.t1 AT1G09970 27.848 158 100 3 40 188 394 546 4.56e-11 63.5
MS.gene029717.t1 AT1G09970 27.848 158 100 3 40 188 394 546 4.60e-11 63.5
MS.gene029717.t1 AT5G49660 25.455 165 103 2 41 205 397 541 4.76e-11 63.5
MS.gene029717.t1 AT4G08850 28.571 154 90 3 40 193 342 475 5.21e-11 63.5
MS.gene029717.t1 AT5G07180 29.143 175 104 3 42 216 346 500 5.47e-11 63.5
MS.gene029717.t1 AT2G41820 30.921 152 85 3 41 192 215 346 6.04e-11 63.2
MS.gene029717.t1 AT4G20140 27.225 191 108 5 42 231 609 769 6.84e-11 63.2
MS.gene029717.t1 AT2G26330 30.460 174 93 7 33 190 116 277 7.02e-11 63.2
MS.gene029717.t1 AT2G01950 32.593 135 75 4 42 165 238 367 8.01e-11 62.8
MS.gene029717.t1 AT5G12940 30.201 149 84 3 44 192 122 250 8.03e-11 62.4
MS.gene029717.t1 AT2G33060 33.696 184 96 8 49 222 308 475 9.31e-11 62.8

Find 59 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCAGCAAATTCACTTTC+TGG 0.157114 1.2:+47460655 MS.gene029717:intron
TCTTCCCATTCTGATGATTC+TGG 0.198688 1.2:-47461878 None:intergenic
CTTTCTCACAATAATCTTAC+TGG 0.269620 1.2:+47460727 MS.gene029717:CDS
TCATCTTGTGTGAACATTAC+TGG 0.281555 1.2:-47461773 None:intergenic
CACGTGTTTCAGTGTTGTTC+AGG 0.284719 1.2:+47460858 MS.gene029717:CDS
AGTGATTCCGTTGGCGATTT+CGG 0.289985 1.2:+47460678 MS.gene029717:CDS
CTTGGTGGAAATCGATTCAT+TGG 0.336277 1.2:+47461148 MS.gene029717:CDS
GGTTCAGTTTCGGCGAGTTT+TGG 0.340741 1.2:+47461169 MS.gene029717:CDS
TACTGGATCAATTCCTGCTT+CGG 0.351663 1.2:+47460744 MS.gene029717:CDS
CTTCCCATTCTGATGATTCT+GGG 0.360548 1.2:-47461877 None:intergenic
CGTTCCACGCGCCGTCTTTG+AGG 0.388539 1.2:+47460782 MS.gene029717:CDS
TCGATTCATTGGTTCAGTTT+CGG 0.390597 1.2:+47461159 MS.gene029717:CDS
TTCGGCGCTTCCGATGCTTC+AGG 0.395526 1.2:+47460696 MS.gene029717:CDS
CAACTTTGGCATTGTGTTGT+TGG 0.397816 1.2:+47461730 MS.gene029717:CDS
GTGTTGTTGGAACTTCTAAC+AGG 0.399817 1.2:+47461743 MS.gene029717:CDS
GGCCGGGTTGACGGAGTTGA+AGG 0.415138 1.2:+47460982 MS.gene029717:CDS
GAGTCTGATGTTTACAACTT+TGG 0.421414 1.2:+47461716 MS.gene029717:CDS
ATAAGAAAATCCTCATTTGA+TGG 0.422989 1.2:-47460530 None:intergenic
ATAGTTCAGCTTGGATTCAA+TGG 0.438842 1.2:+47460805 MS.gene029717:CDS
CTTCAAAATCAACAACACTA+AGG 0.454976 1.2:-47461060 None:intergenic
CTTGCTTGTACAATCCATAA+AGG 0.455824 1.2:-47460553 None:intergenic
GCTCTCTGTCAGCTTGACTT+TGG 0.473613 1.2:-47460507 None:intergenic
GGTGAAGAAACAACTTCAAA+GGG 0.485087 1.2:+47461811 MS.gene029717:CDS
GCTTGGATTCAATGGTTTCA+CGG 0.485189 1.2:+47460813 MS.gene029717:CDS
CTGTGGAAATAATGAAATGT+AGG 0.494608 1.2:+47461035 MS.gene029717:CDS
AATGGTTTCACGGATTTCGT+TGG 0.496182 1.2:+47460823 MS.gene029717:CDS
AGACTCTTTCGTGATTTCCC+TGG 0.498893 1.2:-47461699 None:intergenic
CTCTGTCAGCTTGACTTTGG+CGG 0.500996 1.2:-47460504 None:intergenic
CGACAATGTGAAAGGGCTCA+AGG 0.501698 1.2:+47461117 MS.gene029717:CDS
CACCAGAAGCAATCTTAACC+AGG 0.505162 1.2:+47461681 MS.gene029717:intron
AGACCCAGAATCATCAGAAT+GGG 0.506179 1.2:+47461874 MS.gene029717:CDS
GTGGCTAATAATTCATTCAC+TGG 0.513780 1.2:+47461004 MS.gene029717:CDS
CTACATTTCATTATTTCCAC+AGG 0.519654 1.2:-47461034 None:intergenic
TAGACCCAGAATCATCAGAA+TGG 0.520121 1.2:+47461873 MS.gene029717:CDS
GGGTGAAGAAACAACTTCAA+AGG 0.534035 1.2:+47461810 MS.gene029717:CDS
TTCAAAATCAACAACACTAA+GGG 0.538910 1.2:-47461059 None:intergenic
TAAGAAAATCCTCATTTGAT+GGG 0.539214 1.2:-47460529 None:intergenic
ATTCACTGGTGTTATTCCTG+TGG 0.540433 1.2:+47461018 MS.gene029717:CDS
CACCTTCAACTCCGTCAACC+CGG 0.540616 1.2:-47460984 None:intergenic
CTATCCTCAAAGACGGCGCG+TGG 0.542884 1.2:-47460786 None:intergenic
AGTGACATCACCTGAAGCAT+CGG 0.557850 1.2:-47460706 None:intergenic
GACAGAGAGCCCATCAAATG+AGG 0.569831 1.2:+47460520 MS.gene029717:CDS
TTGTACAAGCAAGCCACTGT+TGG 0.573384 1.2:+47460563 MS.gene029717:CDS
TTCAACTCCGTCAACCCGGC+CGG 0.573761 1.2:-47460980 None:intergenic
TCTTTGAGGATAGTTCAGCT+TGG 0.575408 1.2:+47460796 MS.gene029717:CDS
ATAACAGAGTTACTAGAACC+TGG 0.575865 1.2:+47461839 MS.gene029717:CDS
ACTTTCTGGAGTGATTCCGT+TGG 0.577234 1.2:+47460669 MS.gene029717:CDS
GGGTCTAATTCAAGTAAACC+AGG 0.597683 1.2:-47461857 None:intergenic
AACAACACTGAAACACGTGT+TGG 0.604323 1.2:-47460854 None:intergenic
CGGGTTGACGGAGTTGAAGG+TGG 0.610765 1.2:+47460985 MS.gene029717:CDS
AGCTGAACTATCCTCAAAGA+CGG 0.619300 1.2:-47460793 None:intergenic
ACCAGAAGCAATCTTAACCA+GGG 0.629809 1.2:+47461682 MS.gene029717:intron
GTTGGTTAATGTGATGCAGG+AGG 0.633029 1.2:+47460466 None:intergenic
AGATGAAGACATTGTGAAGT+GGG 0.645221 1.2:+47461790 MS.gene029717:CDS
AAGATGAAGACATTGTGAAG+TGG 0.660204 1.2:+47461789 MS.gene029717:CDS
GGAAGCGCCGAAATCGCCAA+CGG 0.667704 1.2:-47460685 None:intergenic
GGTGTCAACATTTCCAACAG+TGG 0.675818 1.2:-47460576 None:intergenic
ACTGTTGGAAATGTTGACAC+CGG 0.698825 1.2:+47460578 MS.gene029717:CDS
GTGAAGAAACAACTTCAAAG+GGG 0.745434 1.2:+47461812 MS.gene029717:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATTTATGATAACAACTAT+TGG + chr1.2:47461364-47461383 MS.gene029717:intron 20.0%
!! TTGAGAAGTAATAGTTTAAA+TGG + chr1.2:47461220-47461239 MS.gene029717:intron 20.0%
! ATAAGAAAATCCTCATTTGA+TGG - chr1.2:47460533-47460552 None:intergenic 25.0%
! ATTCAACATAATAGCATAAG+AGG + chr1.2:47460889-47460908 MS.gene029717:intron 25.0%
! GTGACATTTATGATAGTAAT+GGG + chr1.2:47461314-47461333 MS.gene029717:intron 25.0%
! TAAGAAAATCCTCATTTGAT+GGG - chr1.2:47460532-47460551 None:intergenic 25.0%
! TTCAAAATCAACAACACTAA+GGG - chr1.2:47461062-47461081 None:intergenic 25.0%
! TTTGAGAAATTTGACAACTT+TGG + chr1.2:47461267-47461286 MS.gene029717:intron 25.0%
!! AAGTGCATTTATCAGATTTT+GGG + chr1.2:47461582-47461601 MS.gene029717:intron 25.0%
!! TCAATGTTTCAAGTAAAGAA+AGG - chr1.2:47461198-47461217 None:intergenic 25.0%
CTACATTTCATTATTTCCAC+AGG - chr1.2:47461037-47461056 None:intergenic 30.0%
CTGTGGAAATAATGAAATGT+AGG + chr1.2:47461035-47461054 MS.gene029717:CDS 30.0%
CTTAGTGATAACAAGTTTAG+TGG + chr1.2:47461292-47461311 MS.gene029717:intron 30.0%
CTTCAAAATCAACAACACTA+AGG - chr1.2:47461063-47461082 None:intergenic 30.0%
CTTTCTCACAATAATCTTAC+TGG + chr1.2:47460727-47460746 MS.gene029717:CDS 30.0%
GGTGACATTTATGATAGTAA+TGG + chr1.2:47461313-47461332 MS.gene029717:intron 30.0%
TTCACATTGTCGAAAAATGT+CGG - chr1.2:47461109-47461128 None:intergenic 30.0%
! AAGATGGTCATGTTCTAAAT+TGG + chr1.2:47461444-47461463 MS.gene029717:intron 30.0%
! AATTTAGAACATGACCATCT+TGG - chr1.2:47461445-47461464 None:intergenic 30.0%
! GAAGTGCATTTATCAGATTT+TGG + chr1.2:47461581-47461600 MS.gene029717:intron 30.0%
! GAGGATTTTCTTATCCTTTA+TGG + chr1.2:47460539-47460558 MS.gene029717:CDS 30.0%
! TTTGAAGGAAACAATTTTGC+CGG + chr1.2:47461076-47461095 MS.gene029717:CDS 30.0%
! TTTTTACTAGGTGTGAAAGT+TGG + chr1.2:47461902-47461921 MS.gene029717:CDS 30.0%
!! ACATTTTTCGACAATGTGAA+AGG + chr1.2:47461109-47461128 MS.gene029717:CDS 30.0%
!! CATTTTTCGACAATGTGAAA+GGG + chr1.2:47461110-47461129 MS.gene029717:CDS 30.0%
!!! CTTAGTGTTGTTGATTTTGA+AGG + chr1.2:47461061-47461080 MS.gene029717:CDS 30.0%
!!! TTTTGGGTTAGAAAGACTAA+CGG + chr1.2:47461598-47461617 MS.gene029717:intron 30.0%
AAGATGAAGACATTGTGAAG+TGG + chr1.2:47461789-47461808 MS.gene029717:CDS 35.0%
AGATGAAGACATTGTGAAGT+GGG + chr1.2:47461790-47461809 MS.gene029717:CDS 35.0%
AGCTCGAAATTATCACTTAC+CGG - chr1.2:47460600-47460619 None:intergenic 35.0%
ATAACAGAGTTACTAGAACC+TGG + chr1.2:47461839-47461858 MS.gene029717:CDS 35.0%
ATAGTTCAGCTTGGATTCAA+TGG + chr1.2:47460805-47460824 MS.gene029717:CDS 35.0%
CTTGCTTGTACAATCCATAA+AGG - chr1.2:47460556-47460575 None:intergenic 35.0%
GAGTCTGATGTTTACAACTT+TGG + chr1.2:47461716-47461735 MS.gene029717:CDS 35.0%
GGTGAAGAAACAACTTCAAA+GGG + chr1.2:47461811-47461830 MS.gene029717:CDS 35.0%
GTGAAGAAACAACTTCAAAG+GGG + chr1.2:47461812-47461831 MS.gene029717:CDS 35.0%
GTGGCTAATAATTCATTCAC+TGG + chr1.2:47461004-47461023 MS.gene029717:CDS 35.0%
TCATCTTGTGTGAACATTAC+TGG - chr1.2:47461776-47461795 None:intergenic 35.0%
! AGTTACATTGGTTAACCACA+AGG - chr1.2:47460923-47460942 None:intergenic 35.0%
! GAATGGGAAGAGTTTTTACT+AGG + chr1.2:47461890-47461909 MS.gene029717:CDS 35.0%
! GTTACATTGGTTAACCACAA+GGG - chr1.2:47460922-47460941 None:intergenic 35.0%
!! CTTTCGAGTAATGCATTTTC+CGG + chr1.2:47460961-47460980 MS.gene029717:CDS 35.0%
!! TCGATTCATTGGTTCAGTTT+CGG + chr1.2:47461159-47461178 MS.gene029717:CDS 35.0%
AACAACACTGAAACACGTGT+TGG - chr1.2:47460857-47460876 None:intergenic 40.0%
AATGGTTTCACGGATTTCGT+TGG + chr1.2:47460823-47460842 MS.gene029717:CDS 40.0%
ACATTGCAAAAAACCGAAGC+AGG - chr1.2:47460760-47460779 None:intergenic 40.0%
ACCAGAAGCAATCTTAACCA+GGG + chr1.2:47461682-47461701 MS.gene029717:intron 40.0%
ACTCCAGAAAGTGAATTTGC+TGG - chr1.2:47460661-47460680 None:intergenic 40.0%
ACTGTTGGAAATGTTGACAC+CGG + chr1.2:47460578-47460597 MS.gene029717:CDS 40.0%
AGACCCAGAATCATCAGAAT+GGG + chr1.2:47461874-47461893 MS.gene029717:CDS 40.0%
AGCTGAACTATCCTCAAAGA+CGG - chr1.2:47460796-47460815 None:intergenic 40.0%
AGTGTTGCAAGGTTTCCATT+GGG - chr1.2:47461404-47461423 None:intergenic 40.0%
CAACTTTGGCATTGTGTTGT+TGG + chr1.2:47461730-47461749 MS.gene029717:CDS 40.0%
CACTGACAGAGTAGTTACAT+TGG - chr1.2:47460935-47460954 None:intergenic 40.0%
CTTCATACACCAATCCACAA+TGG + chr1.2:47461511-47461530 MS.gene029717:intron 40.0%
CTTCCCATTCTGATGATTCT+GGG - chr1.2:47461880-47461899 None:intergenic 40.0%
GAAAAATGTCGGAACTTCAC+CGG - chr1.2:47461098-47461117 None:intergenic 40.0%
GCTTGGATTCAATGGTTTCA+CGG + chr1.2:47460813-47460832 MS.gene029717:CDS 40.0%
GGGTCTAATTCAAGTAAACC+AGG - chr1.2:47461860-47461879 None:intergenic 40.0%
GGGTGAAGAAACAACTTCAA+AGG + chr1.2:47461810-47461829 MS.gene029717:CDS 40.0%
TACTGGATCAATTCCTGCTT+CGG + chr1.2:47460744-47460763 MS.gene029717:CDS 40.0%
TAGACCCAGAATCATCAGAA+TGG + chr1.2:47461873-47461892 MS.gene029717:CDS 40.0%
TCCATTGGGCATGTAATCAT+AGG - chr1.2:47461390-47461409 None:intergenic 40.0%
TCTCCAGCAAATTCACTTTC+TGG + chr1.2:47460655-47460674 MS.gene029717:intron 40.0%
TCTTCCCATTCTGATGATTC+TGG - chr1.2:47461881-47461900 None:intergenic 40.0%
TGTGCTAACTGATGTTGAAG+TGG - chr1.2:47461648-47461667 None:intergenic 40.0%
! CTTGGTGGAAATCGATTCAT+TGG + chr1.2:47461148-47461167 MS.gene029717:CDS 40.0%
! GCGTCAAATAGAACGTTTTG+AGG - chr1.2:47461554-47461573 None:intergenic 40.0%
! GTGTTGTTGGAACTTCTAAC+AGG + chr1.2:47461743-47461762 MS.gene029717:CDS 40.0%
! TCTTTGAGGATAGTTCAGCT+TGG + chr1.2:47460796-47460815 MS.gene029717:CDS 40.0%
! TTTCAGTGTTGTTCAGGTGT+TGG + chr1.2:47460864-47460883 MS.gene029717:intron 40.0%
!! ATTCACTGGTGTTATTCCTG+TGG + chr1.2:47461018-47461037 MS.gene029717:CDS 40.0%
!! CATTGTGGATTGGTGTATGA+AGG - chr1.2:47461513-47461532 None:intergenic 40.0%
!! CTCAAGGTTTTATCACTTGG+TGG + chr1.2:47461133-47461152 MS.gene029717:CDS 40.0%
AGACTCTTTCGTGATTTCCC+TGG - chr1.2:47461702-47461721 None:intergenic 45.0%
AGTGACATCACCTGAAGCAT+CGG - chr1.2:47460709-47460728 None:intergenic 45.0%
AGTGATTCCGTTGGCGATTT+CGG + chr1.2:47460678-47460697 MS.gene029717:CDS 45.0%
ATGAGACACCTCATCACACT+AGG + chr1.2:47461470-47461489 MS.gene029717:intron 45.0%
CAAGAAGCTTCTCACCAAGA+TGG + chr1.2:47461428-47461447 MS.gene029717:intron 45.0%
CACCAGAAGCAATCTTAACC+AGG + chr1.2:47461681-47461700 MS.gene029717:intron 45.0%
CATCTTGGTGAGAAGCTTCT+TGG - chr1.2:47461430-47461449 None:intergenic 45.0%
GCCTATGATTACATGCCCAA+TGG + chr1.2:47461386-47461405 MS.gene029717:intron 45.0%
GCTAACTGATGTTGAAGTGG+AGG - chr1.2:47461645-47461664 None:intergenic 45.0%
GGTGTCAACATTTCCAACAG+TGG - chr1.2:47460579-47460598 None:intergenic 45.0%
TCCCTGGTTAAGATTGCTTC+TGG - chr1.2:47461686-47461705 None:intergenic 45.0%
TTGTACAAGCAAGCCACTGT+TGG + chr1.2:47460563-47460582 MS.gene029717:CDS 45.0%
! AGTGTGATGAGGTGTCTCAT+TGG - chr1.2:47461470-47461489 None:intergenic 45.0%
! CACGTGTTTCAGTGTTGTTC+AGG + chr1.2:47460858-47460877 MS.gene029717:CDS 45.0%
! GAGTGTTGCAAGGTTTCCAT+TGG - chr1.2:47461405-47461424 None:intergenic 45.0%
! TTTGAGGTTTAACGTCACCG+TGG - chr1.2:47461538-47461557 None:intergenic 45.0%
!! ACTTTCTGGAGTGATTCCGT+TGG + chr1.2:47460669-47460688 MS.gene029717:CDS 45.0%
!! GGGCTCAAGGTTTTATCACT+TGG + chr1.2:47461130-47461149 MS.gene029717:CDS 45.0%
ACTCTGTCACCTTCCTACGT+CGG + chr1.2:47460627-47460646 MS.gene029717:intron 50.0%
ATCACACTAGGAATTGCCCG+CGG + chr1.2:47461482-47461501 MS.gene029717:intron 50.0%
CACCAATCCACAATGGTCCA+CGG + chr1.2:47461518-47461537 MS.gene029717:intron 50.0%
CGACAATGTGAAAGGGCTCA+AGG + chr1.2:47461117-47461136 MS.gene029717:CDS 50.0%
CTCTGTCAGCTTGACTTTGG+CGG - chr1.2:47460507-47460526 None:intergenic 50.0%
CTTGGTGAGAAGCTTCTTGG+AGG - chr1.2:47461427-47461446 None:intergenic 50.0%
GACAGAGAGCCCATCAAATG+AGG + chr1.2:47460520-47460539 MS.gene029717:CDS 50.0%
GAGGCTGCTTCTCATGATGT+GGG - chr1.2:47461626-47461645 None:intergenic 50.0%
GCTCTCTGTCAGCTTGACTT+TGG - chr1.2:47460510-47460529 None:intergenic 50.0%
GGGCAATTCCTAGTGTGATG+AGG - chr1.2:47461481-47461500 None:intergenic 50.0%
GGTGTATGAAGGCTAATCCG+CGG - chr1.2:47461502-47461521 None:intergenic 50.0%
GTGTATGAAGGCTAATCCGC+GGG - chr1.2:47461501-47461520 None:intergenic 50.0%
! CGAGTAATGCATTTTCCGGC+CGG + chr1.2:47460965-47460984 MS.gene029717:CDS 50.0%
! GAGTAATGCATTTTCCGGCC+GGG + chr1.2:47460966-47460985 MS.gene029717:CDS 50.0%
! GGTTCAGTTTCGGCGAGTTT+TGG + chr1.2:47461169-47461188 MS.gene029717:CDS 50.0%
! TAAGAGGCACGTTTCCCTTG+TGG + chr1.2:47460905-47460924 MS.gene029717:intron 50.0%
!! CTTCTTGGAGGAGTGTTGCA+AGG - chr1.2:47461415-47461434 None:intergenic 50.0%
AACGTCACCGTGGACCATTG+TGG - chr1.2:47461528-47461547 None:intergenic 55.0%
CACCGTGGACCATTGTGGAT+TGG - chr1.2:47461523-47461542 None:intergenic 55.0%
CACCTTCAACTCCGTCAACC+CGG - chr1.2:47460987-47461006 None:intergenic 55.0%
GAGATCTGTCCGACGTAGGA+AGG - chr1.2:47460639-47460658 None:intergenic 55.0%
GGAGGCTGCTTCTCATGATG+TGG - chr1.2:47461627-47461646 None:intergenic 55.0%
CTATCCTCAAAGACGGCGCG+TGG - chr1.2:47460789-47460808 None:intergenic 60.0%
GCTGGAGATCTGTCCGACGT+AGG - chr1.2:47460643-47460662 None:intergenic 60.0%
TTCAACTCCGTCAACCCGGC+CGG - chr1.2:47460983-47461002 None:intergenic 60.0%
TTCGGCGCTTCCGATGCTTC+AGG + chr1.2:47460696-47460715 MS.gene029717:CDS 60.0%
! CGGGTTGACGGAGTTGAAGG+TGG + chr1.2:47460985-47461004 MS.gene029717:CDS 60.0%
!! GCATTTTCCGGCCGGGTTGA+CGG + chr1.2:47460973-47460992 MS.gene029717:CDS 60.0%
!! GGAAGCGCCGAAATCGCCAA+CGG - chr1.2:47460688-47460707 None:intergenic 60.0%
CGTTCCACGCGCCGTCTTTG+AGG + chr1.2:47460782-47460801 MS.gene029717:CDS 65.0%
! GGCCGGGTTGACGGAGTTGA+AGG + chr1.2:47460982-47461001 MS.gene029717:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 47460479 47461955 47460479 ID=MS.gene029717
chr1.2 mRNA 47460479 47461955 47460479 ID=MS.gene029717.t1;Parent=MS.gene029717
chr1.2 exon 47460479 47460599 47460479 ID=MS.gene029717.t1.exon1;Parent=MS.gene029717.t1
chr1.2 CDS 47460479 47460599 47460479 ID=cds.MS.gene029717.t1;Parent=MS.gene029717.t1
chr1.2 exon 47460662 47460879 47460662 ID=MS.gene029717.t1.exon2;Parent=MS.gene029717.t1
chr1.2 CDS 47460662 47460879 47460662 ID=cds.MS.gene029717.t1;Parent=MS.gene029717.t1
chr1.2 exon 47460953 47461190 47460953 ID=MS.gene029717.t1.exon3;Parent=MS.gene029717.t1
chr1.2 CDS 47460953 47461190 47460953 ID=cds.MS.gene029717.t1;Parent=MS.gene029717.t1
chr1.2 exon 47461687 47461955 47461687 ID=MS.gene029717.t1.exon4;Parent=MS.gene029717.t1
chr1.2 CDS 47461687 47461955 47461687 ID=cds.MS.gene029717.t1;Parent=MS.gene029717.t1
Gene Sequence

>MS.gene029717

ATGCAGGAGGATTTTGAGCAGCATGCCGCCAAAGTCAAGCTGACAGAGAGCCCATCAAATGAGGATTTTCTTATCCTTTATGGATTGTACAAGCAAGCCACTGTTGGAAATGTTGACACCGGTAAGTGATAATTTCGAGCTTGAGTTCACTCTGTCACCTTCCTACGTCGGACAGATCTCCAGCAAATTCACTTTCTGGAGTGATTCCGTTGGCGATTTCGGCGCTTCCGATGCTTCAGGTGATGTCACTTTCTCACAATAATCTTACTGGATCAATTCCTGCTTCGGTTTTTTGCAATGTTTCGTTCCACGCGCCGTCTTTGAGGATAGTTCAGCTTGGATTCAATGGTTTCACGGATTTCGTTGGATTTGAAACCAACACGTGTTTCAGTGTTGTTCAGGTGTTGGATATTCAACATAATAGCATAAGAGGCACGTTTCCCTTGTGGTTAACCAATGTAACTACTCTGTCAGTGCTTGATCTTTCGAGTAATGCATTTTCCGGCCGGGTTGACGGAGTTGAAGGTGGCTAATAATTCATTCACTGGTGTTATTCCTGTGGAAATAATGAAATGTAGGTCCCTTAGTGTTGTTGATTTTGAAGGAAACAATTTTGCCGGTGAAGTTCCGACATTTTTCGACAATGTGAAAGGGCTCAAGGTTTTATCACTTGGTGGAAATCGATTCATTGGTTCAGTTTCGGCGAGTTTTGGTAACCTTTCTTTACTTGAAACATTGAGTTTGAGAAGTAATAGTTTAAATGGAACTATGTTTGAGATGATATTGAGTTTGAGAAATTTGACAACTTTGGATCTTAGTGATAACAAGTTTAGTGGTGACATTTATGATAGTAATGGGAATTTGAATAGATTAATGCTTCTCGTGACATTTATGATAACAACTATTGGCCTATGATTACATGCCCAATGGAAACCTTGCAACACTCCTCCAAGAAGCTTCTCACCAAGATGGTCATGTTCTAAATTGGCCAATGAGACACCTCATCACACTAGGAATTGCCCGCGGATTAGCCTTCATACACCAATCCACAATGGTCCACGGTGACGTTAAACCTCAAAACGTTCTATTTGACGCTGATTTCGAAGTGCATTTATCAGATTTTGGGTTAGAAAGACTAACGGTACCCACATCATGAGAAGCAGCCTCCACTTCAACATCAGTTAGCACATTGAGTTATGTTTCACCAGAAGCAATCTTAACCAGGGAAATCACGAAAGAGTCTGATGTTTACAACTTTGGCATTGTGTTGTTGGAACTTCTAACAGGAAAGAGACCAGTAATGTTCACACAAGATGAAGACATTGTGAAGTGGGTGAAGAAACAACTTCAAAGGGGTCAAATAACAGAGTTACTAGAACCTGGTTTACTTGAATTAGACCCAGAATCATCAGAATGGGAAGAGTTTTTACTAGGTGTGAAAGTTGGTTTGCTAGAGAAAGCTAGAGTGAATCTTTAA
Protein sequence

>MS.gene029717.t1

MQEDFEQHAAKVKLTESPSNEDFLILYGLYKQATVGNVDTANSLSGVIPLAISALPMLQVMSLSHNNLTGSIPASVFCNVSFHAPSLRIVQLGFNGFTDFVGFETNTCFSVVQCLIFRVMHFPAGLTELKVANNSFTGVIPVEIMKCRSLSVVDFEGNNFAGEVPTFFDNVKGLKVLSLGGNRFIGSVSASFEAILTREITKESDVYNFGIVLLELLTGKRPVMFTQDEDIVKWVKKQLQRGQITELLEPGLLELDPESSEWEEFLLGVKVGLLEKARVNL