AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene029833

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029833.t1 MTR_1g064430 84.672 685 48 7 1 633 5 684 0.0 1117
MS.gene029833.t1 MTR_1g064430 84.467 676 48 7 10 633 1 671 0.0 1096
MS.gene029833.t1 MTR_2g094570 48.656 707 258 20 1 633 31 706 0.0 578
MS.gene029833.t1 MTR_2g094570 50.000 678 235 19 29 633 4 650 0.0 577
MS.gene029833.t1 MTR_2g094570 50.000 678 235 19 29 633 4 650 0.0 575
MS.gene029833.t1 MTR_4g058930 50.368 679 221 18 33 633 9 649 0.0 548
MS.gene029833.t1 MTR_4g058930 50.368 679 221 18 33 633 9 649 0.0 548
MS.gene029833.t1 MTR_7g101280 46.512 602 248 15 27 571 22 606 1.90e-158 470
MS.gene029833.t1 MTR_0041s0110 46.512 602 248 15 27 571 22 606 1.90e-158 470
MS.gene029833.t1 MTR_1g094960 42.040 647 241 15 10 564 1 605 1.33e-139 421
MS.gene029833.t1 MTR_7g101275 51.212 330 109 5 45 363 1 289 5.58e-101 313
MS.gene029833.t1 MTR_0041s0120 49.394 330 127 6 45 363 1 301 5.51e-93 293
MS.gene029833.t1 MTR_3g073420 44.986 349 151 9 33 360 16 344 9.93e-76 253
MS.gene029833.t1 MTR_5g061220 40.571 350 159 9 31 358 14 336 4.19e-70 236
MS.gene029833.t1 MTR_2g024430 38.395 349 136 10 33 358 17 309 6.56e-62 214
MS.gene029833.t1 MTR_1g058210 35.714 336 154 7 31 350 7 296 5.35e-60 207
MS.gene029833.t1 MTR_5g040880 35.652 345 156 8 30 358 5 299 3.14e-59 207
MS.gene029833.t1 MTR_5g040740 35.838 346 150 7 33 358 9 302 7.35e-57 197
MS.gene029833.t1 MTR_5g082140 35.260 346 153 8 33 358 9 303 6.94e-53 188
MS.gene029833.t1 MTR_8g100050 33.425 365 175 9 15 359 38 354 2.00e-51 189
MS.gene029833.t1 MTR_8g100050 33.425 365 175 9 15 359 38 354 4.10e-51 190
MS.gene029833.t1 MTR_2g093740 36.158 354 158 11 36 364 56 366 5.54e-51 188
MS.gene029833.t1 MTR_2g093740 36.158 354 158 11 36 364 56 366 1.26e-50 187
MS.gene029833.t1 MTR_2g093740 36.158 354 158 11 36 364 56 366 1.68e-50 188
MS.gene029833.t1 MTR_1g024025 32.958 355 173 8 29 364 36 344 2.50e-50 189
MS.gene029833.t1 MTR_2g093740 35.422 367 156 12 36 364 56 379 2.71e-49 183
MS.gene029833.t1 MTR_4g060460 34.659 352 166 7 36 364 54 364 3.90e-49 184
MS.gene029833.t1 MTR_4g060460 34.659 352 166 7 36 364 54 364 3.90e-49 184
MS.gene029833.t1 MTR_2g093740 35.422 367 156 12 36 364 56 379 5.93e-49 183
MS.gene029833.t1 MTR_2g093740 35.422 367 156 12 36 364 56 379 6.51e-49 183
MS.gene029833.t1 MTR_2g018690 42.922 219 106 3 27 228 14 230 6.50e-48 181
MS.gene029833.t1 MTR_4g021580 43.668 229 102 4 20 228 3 224 7.80e-48 177
MS.gene029833.t1 MTR_4g021580 43.668 229 102 4 20 228 3 224 1.10e-47 178
MS.gene029833.t1 MTR_4g124900 41.991 231 116 5 14 228 1 229 1.57e-47 180
MS.gene029833.t1 MTR_4g124900 41.991 231 116 5 14 228 1 229 1.86e-47 180
MS.gene029833.t1 MTR_2g006270 31.287 342 168 10 35 358 12 304 2.10e-47 173
MS.gene029833.t1 MTR_2g043250 42.986 221 108 4 24 228 11 229 2.29e-47 180
MS.gene029833.t1 MTR_4g088210 34.006 347 160 9 32 355 36 336 2.48e-47 177
MS.gene029833.t1 MTR_4g088210 34.006 347 160 9 32 355 36 336 2.81e-47 177
MS.gene029833.t1 MTR_2g014770 46.512 215 89 6 32 226 51 259 3.16e-47 176
MS.gene029833.t1 MTR_2g005240 35.362 345 152 8 36 359 23 317 5.71e-47 176
MS.gene029833.t1 MTR_2g014770 46.512 215 89 6 32 226 51 259 1.14e-46 175
MS.gene029833.t1 MTR_5g076270 40.741 216 109 3 30 228 18 231 2.29e-46 176
MS.gene029833.t1 MTR_3g064050 40.741 216 109 3 30 228 20 233 4.18e-46 175
MS.gene029833.t1 MTR_8g079492 41.743 218 109 3 27 228 14 229 7.24e-46 175
MS.gene029833.t1 MTR_8g079492 41.743 218 109 3 27 228 14 229 7.61e-46 175
MS.gene029833.t1 MTR_8g027440 35.632 348 144 11 36 358 29 321 1.84e-45 172
MS.gene029833.t1 MTR_8g101360 41.784 213 105 3 33 228 28 238 7.47e-45 172
MS.gene029833.t1 MTR_2g006380 30.029 343 170 10 33 358 17 306 1.43e-44 165
MS.gene029833.t1 MTR_7g062540 43.119 218 101 6 24 224 4 215 5.49e-43 164
MS.gene029833.t1 MTR_5g060780 33.333 345 158 12 33 358 9 300 8.22e-43 159
MS.gene029833.t1 MTR_5g460920 33.333 345 158 12 33 358 9 300 7.04e-42 156
MS.gene029833.t1 MTR_5g061890 33.333 345 158 12 33 358 9 300 3.05e-41 155
MS.gene029833.t1 MTR_5g062970 40.104 192 98 6 36 213 38 226 7.28e-41 149
MS.gene029833.t1 MTR_2g093740 38.017 242 94 6 136 364 38 236 6.88e-34 137
MS.gene029833.t1 MTR_2g093740 38.017 242 94 6 136 364 38 236 7.14e-34 137
MS.gene029833.t1 MTR_2g093740 36.863 255 92 7 136 364 38 249 2.52e-32 133
MS.gene029833.t1 MTR_2g093740 36.863 255 92 7 136 364 38 249 2.72e-32 132
MS.gene029833.t1 MTR_8g079492 44.218 147 69 2 93 228 3 147 3.26e-29 124
MS.gene029833.t1 MTR_5g060630 27.714 350 166 9 31 338 7 311 5.05e-29 122
MS.gene029833.t1 MTR_5g460740 27.714 350 166 9 31 338 7 311 5.05e-29 122
MS.gene029833.t1 MTR_8g446900 35.000 160 85 3 82 228 12 165 4.64e-22 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029833.t1 AT4G30080 48.421 665 254 18 29 633 12 647 4.68e-179 523
MS.gene029833.t1 AT2G28350 45.481 686 251 21 28 633 2 644 1.86e-159 473
MS.gene029833.t1 AT2G28350 45.481 686 251 21 28 633 2 644 5.01e-159 473
MS.gene029833.t1 AT1G77850 41.667 348 158 8 31 356 14 338 1.26e-77 258
MS.gene029833.t1 AT1G77850 41.954 348 157 9 31 356 14 338 1.65e-77 258
MS.gene029833.t1 AT2G46530 28.571 665 322 24 11 633 3 556 1.22e-53 194
MS.gene029833.t1 AT2G46530 29.042 637 312 23 36 633 42 577 3.53e-53 193
MS.gene029833.t1 AT1G59750 29.096 653 338 20 36 633 22 604 4.91e-52 190
MS.gene029833.t1 AT5G60450 36.236 356 153 12 36 364 41 349 6.26e-52 191
MS.gene029833.t1 AT5G60450 36.236 356 153 12 36 364 65 373 9.95e-52 191
MS.gene029833.t1 AT1G59750 29.008 655 338 20 36 633 22 606 1.03e-51 189
MS.gene029833.t1 AT2G33860 35.897 351 161 10 32 364 52 356 3.33e-51 187
MS.gene029833.t1 AT1G59750 28.875 658 338 20 36 633 22 609 3.35e-51 188
MS.gene029833.t1 AT1G59750 28.875 658 338 20 36 633 22 609 3.35e-51 188
MS.gene029833.t1 AT1G19850 32.857 350 173 7 33 364 40 345 1.48e-48 183
MS.gene029833.t1 AT1G19850 32.857 350 173 7 33 364 51 356 1.60e-48 182
MS.gene029833.t1 AT5G62000 31.989 372 185 9 11 359 30 356 7.99e-48 180
MS.gene029833.t1 AT5G62000 31.989 372 185 9 11 359 30 356 7.99e-48 180
MS.gene029833.t1 AT5G62000 31.989 372 185 9 11 359 30 356 7.99e-48 180
MS.gene029833.t1 AT5G62000 31.989 372 185 9 11 359 30 356 7.99e-48 180
MS.gene029833.t1 AT5G62000 31.989 372 185 9 11 359 30 356 8.52e-48 180
MS.gene029833.t1 AT5G37020 41.743 218 108 3 28 228 14 229 1.57e-47 179
MS.gene029833.t1 AT5G37020 41.743 218 108 3 28 228 14 229 2.74e-47 178
MS.gene029833.t1 AT1G19220 44.344 221 105 5 24 228 11 229 7.84e-47 178
MS.gene029833.t1 AT3G61830 33.705 359 170 13 18 357 7 316 2.04e-46 173
MS.gene029833.t1 AT4G23980 43.396 212 93 4 36 226 12 217 6.29e-46 172
MS.gene029833.t1 AT4G23980 43.396 212 93 4 36 226 12 217 6.44e-46 172
MS.gene029833.t1 AT1G30330 40.639 219 111 3 27 228 14 230 1.52e-45 174
MS.gene029833.t1 AT1G30330 40.741 216 109 3 30 228 19 232 1.85e-45 174
MS.gene029833.t1 AT1G30330 40.741 216 109 3 30 228 19 232 1.85e-45 174
MS.gene029833.t1 AT5G20730 42.534 221 109 5 24 228 12 230 3.82e-45 173
MS.gene029833.t1 AT5G20730 42.534 221 109 5 24 228 12 230 4.23e-45 173
MS.gene029833.t1 AT5G20730 42.534 221 109 5 24 228 12 230 5.18e-45 173
MS.gene029833.t1 AT1G35240 26.972 634 330 24 36 633 27 563 9.14e-44 166
MS.gene029833.t1 AT1G35520 28.457 622 297 24 36 608 27 549 3.70e-43 164
MS.gene029833.t1 AT1G34410 27.061 643 332 23 36 633 27 577 4.22e-43 164
MS.gene029833.t1 AT1G34310 27.815 604 324 21 36 608 27 549 1.91e-42 162
MS.gene029833.t1 AT1G35540 34.006 347 157 12 36 363 27 320 1.29e-40 157
MS.gene029833.t1 AT1G34390 27.705 610 315 25 36 608 27 547 2.12e-40 156
MS.gene029833.t1 AT1G34170 29.056 413 205 14 36 427 25 370 4.89e-37 145
MS.gene029833.t1 AT1G34170 36.406 217 108 7 36 231 25 232 8.36e-35 138
MS.gene029833.t1 AT1G34170 36.406 217 108 7 36 231 25 232 1.81e-34 138
MS.gene029833.t1 AT2G46530 28.008 532 252 20 135 633 36 469 3.20e-34 137
MS.gene029833.t1 AT3G61830 34.364 291 129 11 80 357 24 265 6.16e-34 137
MS.gene029833.t1 AT2G33860 36.929 241 97 7 136 364 2 199 1.41e-32 131
MS.gene029833.t1 AT1G43950 38.462 208 97 9 36 224 27 222 5.61e-31 121
MS.gene029833.t1 AT5G37020 49.038 104 42 1 136 228 31 134 3.28e-24 108

Find 161 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAAGATGAATATATTTA+TGG 0.118268 1.4:-55322855 MS.gene029833:CDS
GTCTCGAATAAGTTGGTTTA+TGG 0.152750 1.4:-55321809 MS.gene029833:CDS
AGATGGAAATACCAATAAAA+TGG 0.189014 1.4:-55320871 MS.gene029833:CDS
GCATAAACCTCATCTGTTTC+AGG 0.203134 1.4:+55322593 None:intergenic
CCCTCCTCTTCAGAACATTT+TGG 0.208126 1.4:-55322367 MS.gene029833:intron
TGAGAAAGGTGAAAGGTTTA+TGG 0.219953 1.4:+55321330 None:intergenic
CGAAGAAACGGAAGCAAGTT+TGG 0.233645 1.4:-55320754 MS.gene029833:CDS
TAAGCTTAAGCTTGTTCCTT+TGG 0.291537 1.4:-55322570 MS.gene029833:CDS
TGGTTTATGGGAATTGTATC+TGG 0.292586 1.4:-55321796 MS.gene029833:CDS
GAAACGATGGCACTGAAATT+TGG 0.305036 1.4:+55321254 None:intergenic
GGACAATTCATGAAGTTAAC+AGG 0.315804 1.4:+55322671 None:intergenic
TCGTTTCCTAGTAACCTTCT+AGG 0.317283 1.4:-55321238 MS.gene029833:CDS
AGTTCAGGCAGGTCTATTTC+CGG 0.319570 1.4:-55321111 MS.gene029833:CDS
TTCACCTTGGAGGCTTCTTC+AGG 0.328238 1.4:-55321741 MS.gene029833:intron
AAGCTTAAGCTTGTTCCTTT+GGG 0.328422 1.4:-55322569 MS.gene029833:CDS
AGAAGCCTCCAAGGTGAATT+AGG 0.335922 1.4:+55321746 None:intergenic
CTTGTTCCTTTGGGTGGAAA+TGG 0.343258 1.4:-55322560 MS.gene029833:CDS
ATGCAGATTCGATGGTGTTC+TGG 0.343933 1.4:-55321868 MS.gene029833:CDS
ACAATTGACCTTTCTTTACT+TGG 0.344982 1.4:-55320681 MS.gene029833:CDS
GACAATCTCAATTTCTTCCT+TGG 0.347743 1.4:+55321304 None:intergenic
ACCGCTTGAAGGACCGCTTC+TGG 0.350997 1.4:+55321988 None:intergenic
TCTCGAATAAGTTGGTTTAT+GGG 0.357035 1.4:-55321808 MS.gene029833:CDS
AATGTCTTTGCAAATGAAAC+AGG 0.359372 1.4:+55322479 None:intergenic
ATGGCGAGACGTGGAAGTTT+AGG 0.367612 1.4:-55322332 MS.gene029833:CDS
CAGTTGTTGATCCCCTTCTC+TGG 0.367705 1.4:-55321769 MS.gene029833:CDS
GGAGCCAGGCATGCTCATTA+TGG 0.370077 1.4:-55321164 MS.gene029833:CDS
TCATTATGGTCTTCCGATAT+CGG 0.371359 1.4:-55321150 MS.gene029833:CDS
CATGAGGATCCATTACCATT+AGG 0.376847 1.4:-55322144 MS.gene029833:CDS
CTCTCACTAGAGGAGCGTTT+TGG 0.379568 1.4:+55320801 None:intergenic
TTCTTCCTTGGTTGTGAGAA+AGG 0.380986 1.4:+55321316 None:intergenic
CGCGGTTATCCTTATACCAT+TGG 0.383301 1.4:+55320775 None:intergenic
TGAGAGTTTCCAATGGTATA+AGG 0.391430 1.4:-55320784 MS.gene029833:CDS
CCGCTAAAGTTACCGCTTGA+AGG 0.392870 1.4:+55321977 None:intergenic
AACTTGCTTCCGTTTCTTCG+CGG 0.399377 1.4:+55320757 None:intergenic
AATCACAAGAAGCTTCTTGC+CGG 0.403468 1.4:-55322249 MS.gene029833:CDS
CCTGCTGGCATGCAGGAGCC+AGG 0.430634 1.4:-55321178 MS.gene029833:intron
TTTCGGGTAGACAACGGAAT+AGG 0.433921 1.4:+55321206 None:intergenic
TGGTATTTCCATCTGATGAA+TGG 0.434165 1.4:+55320880 None:intergenic
GGCTAATAAGCAACCGTTTG+AGG 0.435944 1.4:-55321956 MS.gene029833:CDS
CCAACAGGATTCCACCCTAA+TGG 0.436228 1.4:+55322129 None:intergenic
AGAAAGCTGGCAGACATGTT+TGG 0.443426 1.4:-55320639 MS.gene029833:CDS
GTGCTCTACCGTGATATATC+TGG 0.445579 1.4:-55320588 MS.gene029833:CDS
GGAATCAAAGGTGAAACCTT+TGG 0.447087 1.4:+55322650 None:intergenic
TACACAGAATGAGAATCAAA+TGG 0.448127 1.4:-55322082 MS.gene029833:CDS
AACTCAGGCGCGAATATGAC+TGG 0.448876 1.4:-55322026 MS.gene029833:CDS
ATGAGGATCCATTACCATTA+GGG 0.450034 1.4:-55322143 MS.gene029833:CDS
TCTGGCCTAATTCACCTTGG+AGG 0.451766 1.4:-55321751 MS.gene029833:CDS
AATCGTCTCAGCACAATACT+AGG 0.452779 1.4:+55322416 None:intergenic
TCTCCACCTCAGTAAAGTTC+AGG 0.453129 1.4:-55321126 MS.gene029833:CDS
ACACAGAATGAGAATCAAAT+GGG 0.454771 1.4:-55322081 MS.gene029833:CDS
TAAGGATAACCGCGAAGAAA+CGG 0.457301 1.4:-55320766 MS.gene029833:CDS
ATGATCAAAAGGCACGAAAC+CGG 0.458029 1.4:+55321088 None:intergenic
GTCATTAGAGATTTGTTGCT+CGG 0.460103 1.4:+55320902 None:intergenic
GTTTGTGATTCTTACCTGAA+TGG 0.461365 1.4:+55320540 None:intergenic
GATGGAAATACCAATAAAAT+GGG 0.463679 1.4:-55320870 MS.gene029833:CDS
CTGAGGTGGAGATCCGATAT+CGG 0.464440 1.4:+55321137 None:intergenic
TGGGATTCGACGAGCGAAGA+AGG 0.469310 1.4:-55322181 MS.gene029833:CDS
CACTGAAATTTGGCCGTCGA+GGG 0.472008 1.4:+55321264 None:intergenic
ATAGGTTGCTCTGACCTAGA+AGG 0.479580 1.4:+55321224 None:intergenic
TGCATTGCCGCCTCTACTAA+AGG 0.482510 1.4:+55321886 None:intergenic
CAACTATGGCATGCTTGTGC+AGG 0.483052 1.4:-55322770 MS.gene029833:CDS
GGATTCCACCCTAATGGTAA+TGG 0.485472 1.4:+55322135 None:intergenic
ATCACAAGAAGCTTCTTGCC+GGG 0.486243 1.4:-55322248 MS.gene029833:CDS
AAAATGGTGATCTTTGTGTT+GGG 0.488407 1.4:-55322200 MS.gene029833:CDS
TTAATAGATAAAACTTTGCA+TGG 0.488914 1.4:+55322629 None:intergenic
AGACGAATCCTCGGTTTCAA+AGG 0.490672 1.4:+55321828 None:intergenic
CGACGAGCGAAGAAGGGTAT+TGG 0.491306 1.4:-55322174 MS.gene029833:CDS
TTCTCTGGCCTAATTCACCT+TGG 0.492130 1.4:-55321754 MS.gene029833:CDS
CCTTTGGGTGGAAATGGTAA+TGG 0.496376 1.4:-55322554 MS.gene029833:CDS
CGAATATGACTGGAGGGAAA+AGG 0.504257 1.4:-55322016 MS.gene029833:CDS
AAAACTTTGCATGGAATCAA+AGG 0.504526 1.4:+55322638 None:intergenic
ATGACTGGAGGGAAAAGGTT+AGG 0.507657 1.4:-55322011 MS.gene029833:CDS
CTTAAGCTTGTTCCTTTGGG+TGG 0.509650 1.4:-55322566 MS.gene029833:CDS
AGTTTAGCAGCATGATCAAA+AGG 0.510146 1.4:+55321077 None:intergenic
CTCTGACCTAGAAGGTTACT+AGG 0.513995 1.4:+55321232 None:intergenic
AAGTTTAGGCATATTTATAG+AGG 0.517507 1.4:-55322318 MS.gene029833:CDS
TGTCTCTTGCTTACTATCCA+TGG 0.518182 1.4:-55321003 MS.gene029833:CDS
CCATTAGGGTGGAATCCTGT+TGG 0.519653 1.4:-55322129 MS.gene029833:CDS
GGAAGCAAGTTTGGAGAGTA+CGG 0.521728 1.4:-55320745 MS.gene029833:CDS
TAAACTTTACTGTTCACTGT+TGG 0.522539 1.4:+55322731 None:intergenic
TGATGAACTGTACAGAAAGC+TGG 0.523736 1.4:-55320652 MS.gene029833:CDS
GGTGTTTAGATCCTCAACTA+TGG 0.524660 1.4:-55322784 MS.gene029833:CDS
GGGTAGTAAATAACCTCAAA+CGG 0.525195 1.4:+55321943 None:intergenic
CATAAGATCCAAGTAAAGAA+AGG 0.525657 1.4:+55320673 None:intergenic
ACGAGGAGTTCCCGAACCAT+CGG 0.527122 1.4:+55320839 None:intergenic
TTCTGTGTAAACCAAGGAAG+AGG 0.532226 1.4:+55322096 None:intergenic
AGGATTCGTCTCGAATAAGT+TGG 0.533362 1.4:-55321816 MS.gene029833:CDS
GAAAATGGTGATCTTTGTGT+TGG 0.533797 1.4:-55322201 MS.gene029833:CDS
ACATTGGAGAGGAACCATTC+AGG 0.535436 1.4:-55320554 None:intergenic
TCTAATGACCATTCATCAGA+TGG 0.537635 1.4:-55320888 MS.gene029833:CDS
AAGACCATAATGAGCATGCC+TGG 0.540953 1.4:+55321160 None:intergenic
GCATGCTTGTGCAGGTGGAA+TGG 0.542227 1.4:-55322762 MS.gene029833:CDS
CCTTCAAGCGGTAACTTTAG+CGG 0.549169 1.4:-55321977 MS.gene029833:CDS
GCGAAGAAGGGTATTGGTGG+TGG 0.550842 1.4:-55322168 MS.gene029833:CDS
GGGATTCGACGAGCGAAGAA+GGG 0.551807 1.4:-55322180 MS.gene029833:CDS
AAAGTTTATTACTTTCCTCA+AGG 0.553230 1.4:-55322716 MS.gene029833:CDS
CTATGGCATGCTTGTGCAGG+TGG 0.553241 1.4:-55322767 MS.gene029833:CDS
TGTGAAGGCGCCTTTAGTAG+AGG 0.555008 1.4:-55321896 MS.gene029833:CDS
AAGGTGAATTAGGCCAGAGA+AGG 0.557096 1.4:+55321756 None:intergenic
TAACTTCATGAATTGTCCAA+AGG 0.561840 1.4:-55322666 MS.gene029833:CDS
AACTCTGGCGTACTTGCGCG+AGG 0.565767 1.4:+55321922 None:intergenic
GCACTGAAATTTGGCCGTCG+AGG 0.570322 1.4:+55321263 None:intergenic
CCATTACCATTTCCACCCAA+AGG 0.573446 1.4:+55322554 None:intergenic
CAAGATGGCCTTTGAAACCG+AGG 0.577088 1.4:-55321836 MS.gene029833:CDS
CGAGCGAAGAAGGGTATTGG+TGG 0.577713 1.4:-55322171 MS.gene029833:CDS
ATTCATCAGAAGAAGTTGAA+AGG 0.580416 1.4:-55322805 MS.gene029833:CDS
AGGTGAATTAGGCCAGAGAA+GGG 0.583510 1.4:+55321757 None:intergenic
GCAGATTATGACACTGTTGG+AGG 0.586921 1.4:-55322521 MS.gene029833:CDS
CACCTCAGTAAAGTTCAGGC+AGG 0.589149 1.4:-55321122 MS.gene029833:CDS
CTGCCTGAACTTTACTGAGG+TGG 0.589800 1.4:+55321123 None:intergenic
AAAGGTGAAAGGTTTATGGA+TGG 0.590026 1.4:+55321334 None:intergenic
CTGAAATTTGGCCGTCGAGG+GGG 0.590791 1.4:+55321266 None:intergenic
TCAAAAGGCACGAAACCGGC+CGG 0.591651 1.4:+55321092 None:intergenic
GGCGCCTTCACACAAAACTC+TGG 0.592521 1.4:+55321907 None:intergenic
CAGGCAGGTCTATTTCCGGC+CGG 0.592530 1.4:-55321107 MS.gene029833:CDS
GTAGTCAAAAGATGCCGCCT+CGG 0.596640 1.4:+55322291 None:intergenic
AAGATGTTCATGGCGAGACG+TGG 0.596686 1.4:-55322341 MS.gene029833:CDS
AGGCGGCAATGCAGATTCGA+TGG 0.597819 1.4:-55321876 MS.gene029833:CDS
TCTGATGCTAACAATGGTGG+AGG 0.600675 1.4:-55322449 MS.gene029833:CDS
GTTACCTGAAGAAGCCTCCA+AGG 0.601497 1.4:+55321737 None:intergenic
GCTGCAGATTATGACACTGT+TGG 0.602356 1.4:-55322524 MS.gene029833:CDS
TTCTGGAATAAGATTCAAGA+TGG 0.603114 1.4:-55321851 MS.gene029833:CDS
GGGTATTGGTGGTGGACATG+AGG 0.604425 1.4:-55322160 MS.gene029833:CDS
TTCTCATTCTGTGTAAACCA+AGG 0.607875 1.4:+55322090 None:intergenic
TGGCCGAAAAGAACAAGCTG+AGG 0.609518 1.4:+55320936 None:intergenic
TTGGTTGTGAGAAAGGTGAA+AGG 0.610407 1.4:+55321323 None:intergenic
CAATCTGATGCTAACAATGG+TGG 0.611120 1.4:-55322452 MS.gene029833:CDS
GCATGTTCAGCATGACCTTG+AGG 0.617891 1.4:+55322701 None:intergenic
TCACAAGAAGCTTCTTGCCG+GGG 0.620934 1.4:-55322247 MS.gene029833:CDS
TCTGGAGCAGTCAAACACAT+TGG 0.623032 1.4:-55320570 MS.gene029833:CDS
ACTTATTCGAGACGAATCCT+CGG 0.623357 1.4:+55321819 None:intergenic
AGACTGAGTAGAACTTGCCA+TGG 0.625027 1.4:+55320986 None:intergenic
GCACAAGCATGCCATAGTTG+AGG 0.626456 1.4:+55322773 None:intergenic
GGTAAGACTTGTTGATGACG+AGG 0.626525 1.4:+55320822 None:intergenic
ACTGAAATTTGGCCGTCGAG+GGG 0.629416 1.4:+55321265 None:intergenic
AGGATCCATTACCATTAGGG+TGG 0.632365 1.4:-55322140 MS.gene029833:CDS
CATTAACAACAACATAACAA+TGG 0.634782 1.4:-55322052 MS.gene029833:CDS
GCCAGAAGCGGTCCTTCAAG+CGG 0.635688 1.4:-55321989 MS.gene029833:CDS
ACATAACAATGGTTGAACTC+AGG 0.636109 1.4:-55322041 MS.gene029833:CDS
TTTCTCGAATCAGAAGATGT+CGG 0.638567 1.4:-55320708 MS.gene029833:CDS
GGCTGATCCTGAAACAGATG+AGG 0.642209 1.4:-55322600 MS.gene029833:CDS
TCGAATCAGAAGATGTCGGT+CGG 0.645828 1.4:-55320704 MS.gene029833:CDS
CAGGCGCGAATATGACTGGA+GGG 0.648578 1.4:-55322022 MS.gene029833:CDS
TTTCACCTTTCTCACAACCA+AGG 0.649147 1.4:-55321321 MS.gene029833:CDS
GGAAAAGGTTAGGCCAGAAG+CGG 0.650274 1.4:-55322001 MS.gene029833:CDS
TGACTGCTCCAGATATATCA+CGG 0.657225 1.4:+55320580 None:intergenic
CCATTGGAAACTCTCACTAG+AGG 0.665637 1.4:+55320791 None:intergenic
CCTCTAGTGAGAGTTTCCAA+TGG 0.667381 1.4:-55320791 MS.gene029833:CDS
TCAGGCGCGAATATGACTGG+AGG 0.672052 1.4:-55322023 MS.gene029833:CDS
ACTCAATCTGATGCTAACAA+TGG 0.675509 1.4:-55322455 MS.gene029833:CDS
ACTCTGGCGTACTTGCGCGA+GGG 0.681238 1.4:+55321923 None:intergenic
ATTAGGTTATTAGAGACACT+AGG 0.687638 1.4:+55321053 None:intergenic
GGTGAATTAGGCCAGAGAAG+GGG 0.693414 1.4:+55321758 None:intergenic
AGCAGTCAAACACATTGGAG+AGG 0.695616 1.4:-55320565 MS.gene029833:CDS
AGAAGGTTACTAGGAAACGA+TGG 0.704217 1.4:+55321241 None:intergenic
GAAGGCGCCTTTAGTAGAGG+CGG 0.708805 1.4:-55321893 MS.gene029833:CDS
CACAGAATGAGAATCAAATG+GGG 0.710437 1.4:-55322080 MS.gene029833:CDS
GGTCATGCTGAACATGCATG+TGG 0.714823 1.4:-55322695 MS.gene029833:CDS
TTTATAGAGGAACACCGAGG+CGG 0.715637 1.4:-55322305 MS.gene029833:CDS
GACCTGCCTGAACTTTACTG+AGG 0.725533 1.4:+55321120 None:intergenic
ATATTTATAGAGGAACACCG+AGG 0.862150 1.4:-55322308 MS.gene029833:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATCAAATTTAATAAAATA+TGG + chr1.4:55321720-55321739 None:intergenic 10.0%
!! AATTGCATAATACTTAAATA+TGG - chr1.4:55321756-55321775 MS.gene029833:CDS 15.0%
!! TTTGAAGATGAATATATTTA+TGG - chr1.4:55320575-55320594 MS.gene029833:CDS 15.0%
!!! AGTTTTATCTATTAAGTATA+TGG - chr1.4:55320809-55320828 MS.gene029833:CDS 15.0%
!! TCATTAATTTAGTAATCAAG+TGG + chr1.4:55321854-55321873 None:intergenic 20.0%
!! TTAATAGATAAAACTTTGCA+TGG + chr1.4:55320804-55320823 None:intergenic 20.0%
!! TTTGTATGATTTCATTTGAT+AGG - chr1.4:55321937-55321956 MS.gene029833:CDS 20.0%
!!! ATGATATTTTGTAGGTTAAA+TGG - chr1.4:55322005-55322024 MS.gene029833:CDS 20.0%
!!! GTAGTATTATGATATTTTGT+AGG - chr1.4:55321997-55322016 MS.gene029833:CDS 20.0%
!!! TGATATTTTGTAGGTTAAAT+GGG - chr1.4:55322006-55322025 MS.gene029833:CDS 20.0%
! AAAGTTTATTACTTTCCTCA+AGG - chr1.4:55320714-55320733 MS.gene029833:CDS 25.0%
! AAGTTTAGGCATATTTATAG+AGG - chr1.4:55321112-55321131 MS.gene029833:CDS 25.0%
! AGATGGAAATACCAATAAAA+TGG - chr1.4:55322559-55322578 MS.gene029833:CDS 25.0%
! ATTTACTGCAAAATGTAAGA+AGG - chr1.4:55322038-55322057 MS.gene029833:CDS 25.0%
! ATTTCATTTGATAGGATCAT+AGG - chr1.4:55321945-55321964 MS.gene029833:CDS 25.0%
! CATTAACAACAACATAACAA+TGG - chr1.4:55321378-55321397 MS.gene029833:intron 25.0%
! GATGGAAATACCAATAAAAT+GGG - chr1.4:55322560-55322579 MS.gene029833:CDS 25.0%
! TTTACTGCAAAATGTAAGAA+GGG - chr1.4:55322039-55322058 MS.gene029833:CDS 25.0%
!! GGAAAAATTACCCATTTTAT+TGG + chr1.4:55322573-55322592 None:intergenic 25.0%
AAAACTTTGCATGGAATCAA+AGG + chr1.4:55320795-55320814 None:intergenic 30.0%
AAAATGGTGATCTTTGTGTT+GGG - chr1.4:55321230-55321249 MS.gene029833:CDS 30.0%
AATGTCTTTGCAAATGAAAC+AGG + chr1.4:55320954-55320973 None:intergenic 30.0%
ACAATTGACCTTTCTTTACT+TGG - chr1.4:55322749-55322768 MS.gene029833:CDS 30.0%
ACACAGAATGAGAATCAAAT+GGG - chr1.4:55321349-55321368 MS.gene029833:intron 30.0%
ATTAGGTTATTAGAGACACT+AGG + chr1.4:55322380-55322399 None:intergenic 30.0%
ATTCATCAGAAGAAGTTGAA+AGG - chr1.4:55320625-55320644 MS.gene029833:CDS 30.0%
CATAAGATCCAAGTAAAGAA+AGG + chr1.4:55322760-55322779 None:intergenic 30.0%
GAAATCATACAAAACTCAAC+AGG + chr1.4:55321930-55321949 None:intergenic 30.0%
TAAACTTTACTGTTCACTGT+TGG + chr1.4:55320702-55320721 None:intergenic 30.0%
TAACTTCATGAATTGTCCAA+AGG - chr1.4:55320764-55320783 MS.gene029833:CDS 30.0%
TACACAGAATGAGAATCAAA+TGG - chr1.4:55321348-55321367 MS.gene029833:intron 30.0%
TGGCATAGAAAAATATGAGA+AGG - chr1.4:55322811-55322830 MS.gene029833:CDS 30.0%
! AAAATGGGTAATTTTTCCGA+TGG - chr1.4:55322575-55322594 MS.gene029833:CDS 30.0%
! AACAGAAGCACATTCTTAAA+TGG + chr1.4:55321898-55321917 None:intergenic 30.0%
! CTTACATTTTGCAGTAAATC+TGG + chr1.4:55322037-55322056 None:intergenic 30.0%
! TGCTAGATTTTTGCATCATT+AGG + chr1.4:55322397-55322416 None:intergenic 30.0%
!! TCTCGAATAAGTTGGTTTAT+GGG - chr1.4:55321622-55321641 MS.gene029833:intron 30.0%
!! TTCTGGAATAAGATTCAAGA+TGG - chr1.4:55321579-55321598 MS.gene029833:intron 30.0%
!!! ATTTTGGCTAAAGATGTTCA+TGG - chr1.4:55321079-55321098 MS.gene029833:CDS 30.0%
AAAGGTGAAAGGTTTATGGA+TGG + chr1.4:55322099-55322118 None:intergenic 35.0%
ACATAACAATGGTTGAACTC+AGG - chr1.4:55321389-55321408 MS.gene029833:intron 35.0%
ACTCAATCTGATGCTAACAA+TGG - chr1.4:55320975-55320994 MS.gene029833:CDS 35.0%
AGTTTAGCAGCATGATCAAA+AGG + chr1.4:55322356-55322375 None:intergenic 35.0%
ATATTTATAGAGGAACACCG+AGG - chr1.4:55321122-55321141 MS.gene029833:CDS 35.0%
ATGAGGATCCATTACCATTA+GGG - chr1.4:55321287-55321306 MS.gene029833:CDS 35.0%
CACAGAATGAGAATCAAATG+GGG - chr1.4:55321350-55321369 MS.gene029833:intron 35.0%
GAAAATGGTGATCTTTGTGT+TGG - chr1.4:55321229-55321248 MS.gene029833:CDS 35.0%
GACAATCTCAATTTCTTCCT+TGG + chr1.4:55322129-55322148 None:intergenic 35.0%
GGACAATTCATGAAGTTAAC+AGG + chr1.4:55320762-55320781 None:intergenic 35.0%
GGGTAGTAAATAACCTCAAA+CGG + chr1.4:55321490-55321509 None:intergenic 35.0%
TCATTATGGTCTTCCGATAT+CGG - chr1.4:55322280-55322299 MS.gene029833:CDS 35.0%
TCTAATGACCATTCATCAGA+TGG - chr1.4:55322542-55322561 MS.gene029833:CDS 35.0%
TGAGAAAGGTGAAAGGTTTA+TGG + chr1.4:55322103-55322122 None:intergenic 35.0%
TGAGAGTTTCCAATGGTATA+AGG - chr1.4:55322646-55322665 MS.gene029833:CDS 35.0%
TGGTATTTCCATCTGATGAA+TGG + chr1.4:55322553-55322572 None:intergenic 35.0%
TGGTTTATGGGAATTGTATC+TGG - chr1.4:55321634-55321653 MS.gene029833:intron 35.0%
TTCTCATTCTGTGTAAACCA+AGG + chr1.4:55321343-55321362 None:intergenic 35.0%
TTTCTCGAATCAGAAGATGT+CGG - chr1.4:55322722-55322741 MS.gene029833:CDS 35.0%
! AAGCTTAAGCTTGTTCCTTT+GGG - chr1.4:55320861-55320880 MS.gene029833:CDS 35.0%
! AGAGGAAAATTTTCTCCAAC+AGG + chr1.4:55321319-55321338 None:intergenic 35.0%
! GAGAAAATTTTCCTCTTCCT+TGG - chr1.4:55321323-55321342 MS.gene029833:CDS 35.0%
! TAAGCTTAAGCTTGTTCCTT+TGG - chr1.4:55320860-55320879 MS.gene029833:CDS 35.0%
!! AAACCTCAGCTTGTTCTTTT+CGG - chr1.4:55322491-55322510 MS.gene029833:CDS 35.0%
!! GTCATTAGAGATTTGTTGCT+CGG + chr1.4:55322531-55322550 None:intergenic 35.0%
!! GTCTCGAATAAGTTGGTTTA+TGG - chr1.4:55321621-55321640 MS.gene029833:intron 35.0%
!! TTAGCCAAAATGTTCTGAAG+AGG + chr1.4:55321070-55321089 None:intergenic 35.0%
!! TTCGATTGTGTTTTTGAGAG+CGG - chr1.4:55321207-55321226 MS.gene029833:intron 35.0%
AATCACAAGAAGCTTCTTGC+CGG - chr1.4:55321181-55321200 MS.gene029833:intron 40.0%
AATCGTCTCAGCACAATACT+AGG + chr1.4:55321017-55321036 None:intergenic 40.0%
ACTTATTCGAGACGAATCCT+CGG + chr1.4:55321614-55321633 None:intergenic 40.0%
AGAAGGTTACTAGGAAACGA+TGG + chr1.4:55322192-55322211 None:intergenic 40.0%
AGGATTCGTCTCGAATAAGT+TGG - chr1.4:55321614-55321633 MS.gene029833:intron 40.0%
CAAAAACACAATCGAATCCC+CGG + chr1.4:55321203-55321222 None:intergenic 40.0%
CAATCTGATGCTAACAATGG+TGG - chr1.4:55320978-55320997 MS.gene029833:CDS 40.0%
CATGAGGATCCATTACCATT+AGG - chr1.4:55321286-55321305 MS.gene029833:CDS 40.0%
GCATAAACCTCATCTGTTTC+AGG + chr1.4:55320840-55320859 None:intergenic 40.0%
GCTACTAATTCAACCAACCA+TGG + chr1.4:55322070-55322089 None:intergenic 40.0%
GGAATCAAAGGTGAAACCTT+TGG + chr1.4:55320783-55320802 None:intergenic 40.0%
GGTGTTTAGATCCTCAACTA+TGG - chr1.4:55320646-55320665 MS.gene029833:CDS 40.0%
TAAGGATAACCGCGAAGAAA+CGG - chr1.4:55322664-55322683 MS.gene029833:CDS 40.0%
TCGTTTCCTAGTAACCTTCT+AGG - chr1.4:55322192-55322211 MS.gene029833:CDS 40.0%
TGACTGCTCCAGATATATCA+CGG + chr1.4:55322853-55322872 None:intergenic 40.0%
TGATGAACTGTACAGAAAGC+TGG - chr1.4:55322778-55322797 MS.gene029833:CDS 40.0%
TGTCTCTTGCTTACTATCCA+TGG - chr1.4:55322427-55322446 MS.gene029833:CDS 40.0%
TTCTGTGTAAACCAAGGAAG+AGG + chr1.4:55321337-55321356 None:intergenic 40.0%
TTCTTCCTTGGTTGTGAGAA+AGG + chr1.4:55322117-55322136 None:intergenic 40.0%
TTGGTTGTGAGAAAGGTGAA+AGG + chr1.4:55322110-55322129 None:intergenic 40.0%
TTTCACCTTTCTCACAACCA+AGG - chr1.4:55322109-55322128 MS.gene029833:CDS 40.0%
! ATGATCAAAAGGCACGAAAC+CGG + chr1.4:55322345-55322364 None:intergenic 40.0%
! GAAACGATGGCACTGAAATT+TGG + chr1.4:55322179-55322198 None:intergenic 40.0%
! GGGTAATTTTTCCGATGGTT+CGG - chr1.4:55322580-55322599 MS.gene029833:CDS 40.0%
! GGTAATTTTTCCGATGGTTC+GGG - chr1.4:55322581-55322600 MS.gene029833:CDS 40.0%
!! CCAAAATGTTCTGAAGAGGA+GGG + chr1.4:55321066-55321085 None:intergenic 40.0%
!! GTGTTTTTGAGAGCGGAAAA+TGG - chr1.4:55321214-55321233 MS.gene029833:intron 40.0%
!!! GTTGCTCGGTGAGAATTTTT+TGG + chr1.4:55322517-55322536 None:intergenic 40.0%
AAGACCATAATGAGCATGCC+TGG + chr1.4:55322273-55322292 None:intergenic 45.0%
AAGGTGAATTAGGCCAGAGA+AGG + chr1.4:55321677-55321696 None:intergenic 45.0%
AGAAAGCTGGCAGACATGTT+TGG - chr1.4:55322791-55322810 MS.gene029833:CDS 45.0%
AGAAGCCTCCAAGGTGAATT+AGG + chr1.4:55321687-55321706 None:intergenic 45.0%
AGACGAATCCTCGGTTTCAA+AGG + chr1.4:55321605-55321624 None:intergenic 45.0%
AGACTGAGTAGAACTTGCCA+TGG + chr1.4:55322447-55322466 None:intergenic 45.0%
AGCAGTCAAACACATTGGAG+AGG - chr1.4:55322865-55322884 MS.gene029833:CDS 45.0%
AGGATCCATTACCATTAGGG+TGG - chr1.4:55321290-55321309 MS.gene029833:CDS 45.0%
AGGTGAATTAGGCCAGAGAA+GGG + chr1.4:55321676-55321695 None:intergenic 45.0%
AGTTCAGGCAGGTCTATTTC+CGG - chr1.4:55322319-55322338 MS.gene029833:CDS 45.0%
ATAGGTTGCTCTGACCTAGA+AGG + chr1.4:55322209-55322228 None:intergenic 45.0%
ATCACAAGAAGCTTCTTGCC+GGG - chr1.4:55321182-55321201 MS.gene029833:intron 45.0%
ATGACTGGAGGGAAAAGGTT+AGG - chr1.4:55321419-55321438 MS.gene029833:intron 45.0%
ATGCAGATTCGATGGTGTTC+TGG - chr1.4:55321562-55321581 MS.gene029833:intron 45.0%
ATGTAAGAAGGGTGAGTCCA+TGG - chr1.4:55322050-55322069 MS.gene029833:CDS 45.0%
CCATTACCATTTCCACCCAA+AGG + chr1.4:55320879-55320898 None:intergenic 45.0%
CCATTGGAAACTCTCACTAG+AGG + chr1.4:55322642-55322661 None:intergenic 45.0%
CCTCTAGTGAGAGTTTCCAA+TGG - chr1.4:55322639-55322658 MS.gene029833:CDS 45.0%
CCTTCAAGCGGTAACTTTAG+CGG - chr1.4:55321453-55321472 MS.gene029833:intron 45.0%
CCTTTGGGTGGAAATGGTAA+TGG - chr1.4:55320876-55320895 MS.gene029833:CDS 45.0%
CGAAGAAACGGAAGCAAGTT+TGG - chr1.4:55322676-55322695 MS.gene029833:CDS 45.0%
CGAATATGACTGGAGGGAAA+AGG - chr1.4:55321414-55321433 MS.gene029833:intron 45.0%
CGCGGTTATCCTTATACCAT+TGG + chr1.4:55322658-55322677 None:intergenic 45.0%
CTCTGACCTAGAAGGTTACT+AGG + chr1.4:55322201-55322220 None:intergenic 45.0%
CTTGTTCCTTTGGGTGGAAA+TGG - chr1.4:55320870-55320889 MS.gene029833:CDS 45.0%
GCAGATTATGACACTGTTGG+AGG - chr1.4:55320909-55320928 MS.gene029833:CDS 45.0%
GCTGCAGATTATGACACTGT+TGG - chr1.4:55320906-55320925 MS.gene029833:CDS 45.0%
GGAAGCAAGTTTGGAGAGTA+CGG - chr1.4:55322685-55322704 MS.gene029833:CDS 45.0%
GGATTCCACCCTAATGGTAA+TGG + chr1.4:55321298-55321317 None:intergenic 45.0%
GGCTAATAAGCAACCGTTTG+AGG - chr1.4:55321474-55321493 MS.gene029833:intron 45.0%
GTGCTCTACCGTGATATATC+TGG - chr1.4:55322842-55322861 MS.gene029833:CDS 45.0%
TCGAATCAGAAGATGTCGGT+CGG - chr1.4:55322726-55322745 MS.gene029833:CDS 45.0%
TCTCCACCTCAGTAAAGTTC+AGG - chr1.4:55322304-55322323 MS.gene029833:CDS 45.0%
TCTGATGCTAACAATGGTGG+AGG - chr1.4:55320981-55321000 MS.gene029833:CDS 45.0%
TTCTCTGGCCTAATTCACCT+TGG - chr1.4:55321676-55321695 MS.gene029833:intron 45.0%
TTTATAGAGGAACACCGAGG+CGG - chr1.4:55321125-55321144 MS.gene029833:CDS 45.0%
TTTCCCTCGTTTGGATTACG+CGG - chr1.4:55321036-55321055 MS.gene029833:CDS 45.0%
! AACTTGCTTCCGTTTCTTCG+CGG + chr1.4:55322676-55322695 None:intergenic 45.0%
! CCCTCCTCTTCAGAACATTT+TGG - chr1.4:55321063-55321082 MS.gene029833:CDS 45.0%
! CTTAAGCTTGTTCCTTTGGG+TGG - chr1.4:55320864-55320883 MS.gene029833:CDS 45.0%
! GAGTACTTTCGGGTAGACAA+CGG + chr1.4:55322233-55322252 None:intergenic 45.0%
! TCTGGAGCAGTCAAACACAT+TGG - chr1.4:55322860-55322879 MS.gene029833:CDS 45.0%
! TGAGACGATTTTCCCTCGTT+TGG - chr1.4:55321027-55321046 MS.gene029833:CDS 45.0%
! TTTCGGGTAGACAACGGAAT+AGG + chr1.4:55322227-55322246 None:intergenic 45.0%
!! AATGTTCTGAAGAGGAGGGT+CGG + chr1.4:55321062-55321081 None:intergenic 45.0%
!! GCCAAAATGTTCTGAAGAGG+AGG + chr1.4:55321067-55321086 None:intergenic 45.0%
!! GGCATCTTTTGACTACTGGT+TGG - chr1.4:55321146-55321165 MS.gene029833:CDS 45.0%
!! GGTAAGACTTGTTGATGACG+AGG + chr1.4:55322611-55322630 None:intergenic 45.0%
!! TACGCCAGAGTTTTGTGTGA+AGG - chr1.4:55321519-55321538 MS.gene029833:intron 45.0%
AACTCAGGCGCGAATATGAC+TGG - chr1.4:55321404-55321423 MS.gene029833:intron 50.0%
AAGAAGGGTGAGTCCATGGT+TGG - chr1.4:55322054-55322073 MS.gene029833:CDS 50.0%
AAGATGTTCATGGCGAGACG+TGG - chr1.4:55321089-55321108 MS.gene029833:CDS 50.0%
ACTGAAATTTGGCCGTCGAG+GGG + chr1.4:55322168-55322187 None:intergenic 50.0%
ATGGCGAGACGTGGAAGTTT+AGG - chr1.4:55321098-55321117 MS.gene029833:CDS 50.0%
CAACTATGGCATGCTTGTGC+AGG - chr1.4:55320660-55320679 MS.gene029833:CDS 50.0%
CACCTCAGTAAAGTTCAGGC+AGG - chr1.4:55322308-55322327 MS.gene029833:CDS 50.0%
CACTGAAATTTGGCCGTCGA+GGG + chr1.4:55322169-55322188 None:intergenic 50.0%
CAGTTGTTGATCCCCTTCTC+TGG - chr1.4:55321661-55321680 MS.gene029833:intron 50.0%
CCAACAGGATTCCACCCTAA+TGG + chr1.4:55321304-55321323 None:intergenic 50.0%
CCATTAGGGTGGAATCCTGT+TGG - chr1.4:55321301-55321320 MS.gene029833:CDS 50.0%
CCGCTAAAGTTACCGCTTGA+AGG + chr1.4:55321456-55321475 None:intergenic 50.0%
CTGAGGTGGAGATCCGATAT+CGG + chr1.4:55322296-55322315 None:intergenic 50.0%
CTGCCTGAACTTTACTGAGG+TGG + chr1.4:55322310-55322329 None:intergenic 50.0%
GACCTGCCTGAACTTTACTG+AGG + chr1.4:55322313-55322332 None:intergenic 50.0%
GCACAAGCATGCCATAGTTG+AGG + chr1.4:55320660-55320679 None:intergenic 50.0%
GCATGTTCAGCATGACCTTG+AGG + chr1.4:55320732-55320751 None:intergenic 50.0%
GGAAAAGGTTAGGCCAGAAG+CGG - chr1.4:55321429-55321448 MS.gene029833:intron 50.0%
GGCTGATCCTGAAACAGATG+AGG - chr1.4:55320830-55320849 MS.gene029833:CDS 50.0%
GGTCATGCTGAACATGCATG+TGG - chr1.4:55320735-55320754 MS.gene029833:CDS 50.0%
GGTGAATTAGGCCAGAGAAG+GGG + chr1.4:55321675-55321694 None:intergenic 50.0%
GTAGTCAAAAGATGCCGCCT+CGG + chr1.4:55321142-55321161 None:intergenic 50.0%
GTTACCTGAAGAAGCCTCCA+AGG + chr1.4:55321696-55321715 None:intergenic 50.0%
TCACAAGAAGCTTCTTGCCG+GGG - chr1.4:55321183-55321202 MS.gene029833:intron 50.0%
TCTGGCCTAATTCACCTTGG+AGG - chr1.4:55321679-55321698 MS.gene029833:intron 50.0%
TGCATTGCCGCCTCTACTAA+AGG + chr1.4:55321547-55321566 None:intergenic 50.0%
TGGCCGAAAAGAACAAGCTG+AGG + chr1.4:55322497-55322516 None:intergenic 50.0%
TTCACCTTGGAGGCTTCTTC+AGG - chr1.4:55321689-55321708 MS.gene029833:intron 50.0%
!! AGGCGGCATCTTTTGACTAC+TGG - chr1.4:55321142-55321161 MS.gene029833:CDS 50.0%
!! ATGCCAGCAGGAGTACTTTC+GGG + chr1.4:55322243-55322262 None:intergenic 50.0%
!! CAAGATGGCCTTTGAAACCG+AGG - chr1.4:55321594-55321613 MS.gene029833:intron 50.0%
!! CATGCCAGCAGGAGTACTTT+CGG + chr1.4:55322244-55322263 None:intergenic 50.0%
!! TGTGAAGGCGCCTTTAGTAG+AGG - chr1.4:55321534-55321553 MS.gene029833:intron 50.0%
!!! CTCTCACTAGAGGAGCGTTT+TGG + chr1.4:55322632-55322651 None:intergenic 50.0%
AAGTACTCCTGCTGGCATGC+AGG - chr1.4:55322245-55322264 MS.gene029833:CDS 55.0%
ACGAGGAGTTCCCGAACCAT+CGG + chr1.4:55322594-55322613 None:intergenic 55.0%
ACGCGTAATGCACAGTGCGA+AGG + chr1.4:55321818-55321837 None:intergenic 55.0%
AGGCGGCAATGCAGATTCGA+TGG - chr1.4:55321554-55321573 MS.gene029833:intron 55.0%
CAGGCGCGAATATGACTGGA+GGG - chr1.4:55321408-55321427 MS.gene029833:intron 55.0%
CTATGGCATGCTTGTGCAGG+TGG - chr1.4:55320663-55320682 MS.gene029833:CDS 55.0%
CTGAAATTTGGCCGTCGAGG+GGG + chr1.4:55322167-55322186 None:intergenic 55.0%
GCACTGAAATTTGGCCGTCG+AGG + chr1.4:55322170-55322189 None:intergenic 55.0%
GCATGCTTGTGCAGGTGGAA+TGG - chr1.4:55320668-55320687 MS.gene029833:CDS 55.0%
GGAGCCAGGCATGCTCATTA+TGG - chr1.4:55322266-55322285 MS.gene029833:CDS 55.0%
GGCGCCTTCACACAAAACTC+TGG + chr1.4:55321526-55321545 None:intergenic 55.0%
GGGATTCGACGAGCGAAGAA+GGG - chr1.4:55321250-55321269 MS.gene029833:CDS 55.0%
TCAGGCGCGAATATGACTGG+AGG - chr1.4:55321407-55321426 MS.gene029833:intron 55.0%
TGGGATTCGACGAGCGAAGA+AGG - chr1.4:55321249-55321268 MS.gene029833:CDS 55.0%
! CGACGAGCGAAGAAGGGTAT+TGG - chr1.4:55321256-55321275 MS.gene029833:CDS 55.0%
! TCAAAAGGCACGAAACCGGC+CGG + chr1.4:55322341-55322360 None:intergenic 55.0%
!! CGAGCGAAGAAGGGTATTGG+TGG - chr1.4:55321259-55321278 MS.gene029833:CDS 55.0%
!! CTACCCGAAAGTACTCCTGC+TGG - chr1.4:55322237-55322256 MS.gene029833:CDS 55.0%
!! GAAGGCGCCTTTAGTAGAGG+CGG - chr1.4:55321537-55321556 MS.gene029833:intron 55.0%
!! GCGAAGAAGGGTATTGGTGG+TGG - chr1.4:55321262-55321281 MS.gene029833:CDS 55.0%
!! GGGTATTGGTGGTGGACATG+AGG - chr1.4:55321270-55321289 MS.gene029833:CDS 55.0%
AACTCTGGCGTACTTGCGCG+AGG + chr1.4:55321511-55321530 None:intergenic 60.0%
ACCGCTTGAAGGACCGCTTC+TGG + chr1.4:55321445-55321464 None:intergenic 60.0%
ACTCTGGCGTACTTGCGCGA+GGG + chr1.4:55321510-55321529 None:intergenic 60.0%
CACCACGATTTCCCCCTCGA+CGG - chr1.4:55322153-55322172 MS.gene029833:CDS 60.0%
CAGGCAGGTCTATTTCCGGC+CGG - chr1.4:55322323-55322342 MS.gene029833:CDS 60.0%
CGGCCGCGTAATCCAAACGA+GGG + chr1.4:55321042-55321061 None:intergenic 60.0%
GCCAGAAGCGGTCCTTCAAG+CGG - chr1.4:55321441-55321460 MS.gene029833:intron 60.0%
TCGAGGGGGAAATCGTGGTG+TGG + chr1.4:55322153-55322172 None:intergenic 60.0%
TCGGCCGCGTAATCCAAACG+AGG + chr1.4:55321043-55321062 None:intergenic 60.0%
CCTGCTGGCATGCAGGAGCC+AGG - chr1.4:55322252-55322271 MS.gene029833:CDS 70.0%
CCTGGCTCCTGCATGCCAGC+AGG + chr1.4:55322255-55322274 None:intergenic 70.0%
GGCCGTCGAGGGGGAAATCG+TGG + chr1.4:55322158-55322177 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 55320555 55322897 55320555 ID=MS.gene029833
chr1.4 mRNA 55320555 55322897 55320555 ID=MS.gene029833.t1;Parent=MS.gene029833
chr1.4 exon 55322409 55322897 55322409 ID=MS.gene029833.t1.exon1;Parent=MS.gene029833.t1
chr1.4 CDS 55322409 55322897 55322409 ID=cds.MS.gene029833.t1;Parent=MS.gene029833.t1
chr1.4 exon 55321742 55322376 55321742 ID=MS.gene029833.t1.exon2;Parent=MS.gene029833.t1
chr1.4 CDS 55321742 55322376 55321742 ID=cds.MS.gene029833.t1;Parent=MS.gene029833.t1
chr1.4 exon 55321221 55321366 55321221 ID=MS.gene029833.t1.exon3;Parent=MS.gene029833.t1
chr1.4 CDS 55321221 55321366 55321221 ID=cds.MS.gene029833.t1;Parent=MS.gene029833.t1
chr1.4 exon 55320555 55321185 55320555 ID=MS.gene029833.t1.exon4;Parent=MS.gene029833.t1
chr1.4 CDS 55320555 55321185 55320555 ID=cds.MS.gene029833.t1;Parent=MS.gene029833.t1
Gene Sequence

>MS.gene029833

AAATTTTGCAGTGAAAGTTTTTTGAAGATGAATATATTTATGGATTCAAGAGATAATAACAATAATAACAATTCATCAGAAGAAGTTGAAAGGTGTTTAGATCCTCAACTATGGCATGCTTGTGCAGGTGGAATGGTTCAGATGCCAACAGTGAACAGTAAAGTTTATTACTTTCCTCAAGGTCATGCTGAACATGCATGTGGACCTGTTAACTTCATGAATTGTCCAAAGGTTTCACCTTTGATTCCATGCAAAGTTTTATCTATTAAGTATATGGCTGATCCTGAAACAGATGAGGTTTATGCTAAGCTTAAGCTTGTTCCTTTGGGTGGAAATGGTAATGGAAATGATGCTGCAGATTATGACACTGTTGGAGGATATGAAAATCAAGATAAGCCTGTTTCATTTGCAAAGACATTGACTCAATCTGATGCTAACAATGGTGGAGGTTTTTCTGTTCCTAGTATTGTGCTGAGACGATTTTCCCTCGTTTGGATTACGCGGCCGACCCTCCTCTTCAGAACATTTTGGCTAAAGATGTTCATGGCGAGACGTGGAAGTTTAGGCATATTTATAGAGGAACACCGAGGCGGCATCTTTTGACTACTGGTTGGAGTACTTTTGTTAATCACAAGAAGCTTCTTGCCGGGGATTCGATTGTGTTTTTGAGAGCGGAAAATGGTGATCTTTGTGTTGGGATTCGACGAGCGAAGAAGGGTATTGGTGGTGGACATGAGGATCCATTACCATTAGGGTGGAATCCTGTTGGAGAAAATTTTCCTCTTCCTTGGTTTACACAGAATGAGAATCAAATGGGGAGAAACATTAACAACAACATAACAATGGTTGAACTCAGGCGCGAATATGACTGGAGGGAAAAGGTTAGGCCAGAAGCGGTCCTTCAAGCGGTAACTTTAGCGGCTAATAAGCAACCGTTTGAGGTTATTTACTACCCTCGCGCAAGTACGCCAGAGTTTTGTGTGAAGGCGCCTTTAGTAGAGGCGGCAATGCAGATTCGATGGTGTTCTGGAATAAGATTCAAGATGGCCTTTGAAACCGAGGATTCGTCTCGAATAAGTTGGTTTATGGGAATTGTATCTGGAGTTCAGTTGTTGATCCCCTTCTCTGGCCTAATTCACCTTGGAGGCTTCTTCAGGTAACACCATATTTTATTAAATTTGATGTCAATTAACAGAACATAAATTGCATAATACTTAAATATGGTAATCTTCTCTCTTGCTAATCTTTAATTTATGTTATCCTTCGCACTGTGCATTACGCGTACACGTTGAGTTACCACTTGATTACTAAATTAATGAACTCTGATGTTGTTTGTTAATCCATTTAAGAATGTGCTTCTGTTATTTTGTTACCTGTTGAGTTTTGTATGATTTCATTTGATAGGATCATAGGAATATTTGCATTGTGTCTACTCATAATAAGTAGTATTATGATATTTTGTAGGTTAAATGGGATGAACCAGATTTACTGCAAAATGTAAGAAGGGTGAGTCCATGGTTGGTTGAATTAGTAGCAACATTCCATCCATAAACCTTTCACCTTTCTCACAACCAAGGAAGAAATTGAGATTGTCTCCACACCACGATTTCCCCCTCGACGGCCAAATTTCAGTGCCATCGTTTCCTAGTAACCTTCTAGGTCAGAGCAACCTATTCCGTTGTCTACCCGAAAGTACTCCTGCTGGCATGCAGGAGCCAGGCATGCTCATTATGGTCTTCCGATATCGGATCTCCACCTCAGTAAAGTTCAGGCAGGTCTATTTCCGGCCGGTTTCGTGCCTTTTGATCATGCTGCTAAACTACCTAGTGTCTCTAATAACCTAATGATGCAAAAATCTAGCACGAGTGAGAATGTCTCTTGCTTACTATCCATGGCAAGTTCTACTCAGTCTTCAAAGAAATTCAACGATGAGAAGAAACCTCAGCTTGTTCTTTTCGGCCAAAAAATTCTCACCGAGCAACAAATCTCTAATGACCATTCATCAGATGGAAATACCAATAAAATGGGTAATTTTTCCGATGGTTCGGGAACTCCTCGTCATCAACAAGTCTTACCAAAACGCTCCTCTAGTGAGAGTTTCCAATGGTATAAGGATAACCGCGAAGAAACGGAAGCAAGTTTGGAGAGTACGGATCACTGTAAAGTTTTTCTCGAATCAGAAGATGTCGGTCGGACAATTGACCTTTCTTTACTTGGATCTTATGATGAACTGTACAGAAAGCTGGCAGACATGTTTGGCATAGAAAAATATGAGAAGGTAAGTCGCGTGCTCTACCGTGATATATCTGGAGCAGTCAAACACATTGGAGAGGAACCATTCAG
Protein sequence

>MS.gene029833.t1

KFCSESFLKMNIFMDSRDNNNNNNSSEEVERCLDPQLWHACAGGMVQMPTVNSKVYYFPQGHAEHACGPVNFMNCPKVSPLIPCKVLSIKYMADPETDEVYAKLKLVPLGGNGNGNDAADYDTVGGYENQDKPVSFAKTLTQSDANNGGGFSVPSIVLRRFSLNILAKDVHGETWKFRHIYRGTPRRHLLTTGWSTFVNHKKLLAGDSIVFLRAENGDLCVGIRRAKKGIGGGHEDPLPLGWNPVGENFPLPWFTQNENQMGRNINNNITMVELRREYDWREKVRPEAVLQAVTLAANKQPFEVIYYPRASTPEFCVKAPLVEAAMQIRWCSGIRFKMAFETEDSSRISWFMGIVSGVQLLIPFSGLIHLGGFFSSNIPSINLSPFSQPRKKLRLSPHHDFPLDGQISVPSFPSNLLGQSNLFRARHAHYGLPISDLHLSKVQAGLFPAGFVPFDHAAKLPSVSNNLMMQKSSTSENVSCLLSMASSTQSSKKFNDEKKPQLVLFGQKILTEQQISNDHSSDGNTNKMGNFSDGSGTPRHQQVLPKRSSSESFQWYKDNREETEASLESTDHCKVFLESEDVGRTIDLSLLGSYDELYRKLADMFGIEKYEKVSRVLYRDISGAVKHIGEEPF