AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene029847

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029847.t1 MTR_4g059870 47.082 514 204 17 3 487 1 475 5.61e-129 384
MS.gene029847.t1 MTR_2g099990 47.427 447 158 11 38 468 46 431 4.58e-116 350
MS.gene029847.t1 MTR_2g090745 46.983 464 169 18 46 486 15 424 4.02e-112 338
MS.gene029847.t1 MTR_8g017210 88.623 167 18 1 38 204 62 227 7.66e-112 341
MS.gene029847.t1 MTR_2g093960 58.333 288 106 5 38 325 32 305 1.01e-111 337
MS.gene029847.t1 MTR_1g094115 87.952 166 18 2 36 200 44 208 2.11e-109 335
MS.gene029847.t1 MTR_1g016010 52.695 334 76 7 38 369 41 294 4.76e-108 329
MS.gene029847.t1 MTR_1g112270 83.333 168 27 1 41 208 47 213 2.67e-106 328
MS.gene029847.t1 MTR_1g112270 78.212 179 27 2 41 208 47 224 1.17e-102 319
MS.gene029847.t1 MTR_2g099990 45.154 423 155 11 62 468 1 362 3.02e-99 305
MS.gene029847.t1 MTR_1g094115 77.108 166 19 3 36 200 44 191 3.10e-91 288
MS.gene029847.t1 MTR_2g017720 76.724 116 21 2 48 163 323 432 1.01e-55 192
MS.gene029847.t1 MTR_3g102000 64.423 104 29 3 65 164 36 135 5.35e-38 139
MS.gene029847.t1 MTR_5g030560 84.444 45 7 0 48 92 16 60 6.81e-22 90.1
MS.gene029847.t1 MTR_1g093095 34.028 144 83 4 59 191 107 249 4.18e-20 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029847.t1 AT5G03150 50.485 515 213 10 1 488 1 500 1.42e-141 417
MS.gene029847.t1 AT5G66730 53.061 392 127 10 19 374 19 389 4.64e-115 348
MS.gene029847.t1 AT3G50700 86.364 176 21 2 26 201 24 196 6.34e-114 344
MS.gene029847.t1 AT3G45260 53.175 378 123 9 38 393 39 384 1.48e-113 343
MS.gene029847.t1 AT3G45260 53.175 378 123 9 38 393 39 384 1.48e-113 343
MS.gene029847.t1 AT5G44160 50.811 370 153 9 34 398 33 378 5.76e-113 342
MS.gene029847.t1 AT5G60470 73.077 208 49 2 3 205 2 207 1.10e-111 338
MS.gene029847.t1 AT5G60470 73.077 208 49 2 3 205 6 211 1.29e-111 338
MS.gene029847.t1 AT4G02670 83.908 174 28 0 37 210 52 225 3.30e-111 335
MS.gene029847.t1 AT4G02670 83.908 174 28 0 37 210 54 227 4.06e-111 335
MS.gene029847.t1 AT1G55110 88.485 165 19 0 38 202 63 227 4.69e-111 337
MS.gene029847.t1 AT1G55110 88.485 165 19 0 38 202 63 227 4.69e-111 337
MS.gene029847.t1 AT1G55110 88.485 165 19 0 38 202 63 227 4.69e-111 337
MS.gene029847.t1 AT1G03840 50.526 380 116 10 34 368 37 389 1.74e-110 337
MS.gene029847.t1 AT2G02080 50.689 363 146 9 29 372 46 394 1.76e-109 335
MS.gene029847.t1 AT2G02080 50.689 363 146 9 29 372 46 394 1.76e-109 335
MS.gene029847.t1 AT2G02080 50.689 363 146 9 29 372 46 394 1.76e-109 335
MS.gene029847.t1 AT2G02080 50.689 363 146 9 29 372 46 394 1.76e-109 335
MS.gene029847.t1 AT3G13810 85.119 168 25 0 38 205 51 218 3.79e-109 333
MS.gene029847.t1 AT3G13810 85.119 168 25 0 38 205 65 232 4.89e-109 333
MS.gene029847.t1 AT3G13810 85.119 168 25 0 38 205 70 237 5.79e-109 333
MS.gene029847.t1 AT3G13810 85.119 168 25 0 38 205 70 237 5.79e-109 333
MS.gene029847.t1 AT1G03840 50.000 380 116 11 34 368 37 387 7.00e-107 328
MS.gene029847.t1 AT1G14580 78.736 174 36 1 37 210 52 224 1.86e-106 325
MS.gene029847.t1 AT1G14580 78.736 174 36 1 37 210 52 224 1.86e-106 325
MS.gene029847.t1 AT1G14580 78.736 174 36 1 37 210 66 238 2.77e-106 325
MS.gene029847.t1 AT5G44160 50.000 356 149 9 48 398 8 339 7.29e-105 320
MS.gene029847.t1 AT2G02070 83.626 171 26 2 38 207 52 221 8.65e-105 325
MS.gene029847.t1 AT2G02070 83.626 171 26 2 38 207 52 221 8.65e-105 325
MS.gene029847.t1 AT3G13810 85.987 157 22 0 49 205 68 224 3.55e-102 315
MS.gene029847.t1 AT3G13810 85.987 157 22 0 49 205 82 238 4.38e-102 316
MS.gene029847.t1 AT5G60470 82.166 157 28 0 49 205 46 202 4.68e-99 306
MS.gene029847.t1 AT2G02080 48.788 330 137 8 62 372 1 317 2.35e-92 288
MS.gene029847.t1 AT2G02080 48.788 330 137 8 62 372 1 317 2.35e-92 288
MS.gene029847.t1 AT2G01940 65.217 161 55 1 35 194 41 201 3.06e-76 246
MS.gene029847.t1 AT1G25250 63.580 162 57 2 33 192 28 189 2.22e-75 242
MS.gene029847.t1 AT1G68130 63.462 156 56 1 38 192 41 196 2.49e-74 241
MS.gene029847.t1 AT2G01940 64.198 162 56 2 35 194 41 202 1.42e-72 237
MS.gene029847.t1 AT1G25250 65.306 147 49 2 48 192 20 166 7.90e-71 230
MS.gene029847.t1 AT2G01940 63.393 112 40 1 84 194 1 112 7.01e-47 167
MS.gene029847.t1 AT1G25250 63.063 111 39 2 84 192 1 111 4.55e-46 163
MS.gene029847.t1 AT1G68130 61.818 110 41 1 84 192 1 110 5.22e-46 164
MS.gene029847.t1 AT5G22890 32.673 202 118 7 5 188 150 351 1.72e-24 105
MS.gene029847.t1 AT1G34370 34.810 158 90 4 47 191 14 171 1.17e-23 101
MS.gene029847.t1 AT1G34370 34.810 158 90 4 47 191 224 381 2.72e-23 103
MS.gene029847.t1 AT1G34370 34.810 158 90 4 47 191 224 381 2.72e-23 103
MS.gene029847.t1 AT1G13290 35.849 159 89 7 59 205 93 250 1.95e-21 95.1
MS.gene029847.t1 AT3G20880 34.247 146 87 4 54 191 243 387 4.34e-19 89.7
MS.gene029847.t1 AT1G34790 32.948 173 100 5 51 208 129 300 5.45e-19 88.2
MS.gene029847.t1 AT3G57670 31.548 168 98 5 54 205 204 370 2.02e-18 87.4
MS.gene029847.t1 AT1G51220 32.877 146 89 4 54 191 165 309 2.14e-18 86.7
MS.gene029847.t1 AT1G08290 37.037 135 75 4 66 191 179 312 3.51e-18 86.3

Find 92 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAGATTTCTCTTCTTCT+TGG 0.211257 1.4:+53248223 None:intergenic
CACCATGGTGGTTCTGGTTC+TGG 0.254794 1.4:-53246671 MS.gene029847:CDS
GGAATTCCACATCCACAATT+TGG 0.260215 1.4:-53247280 MS.gene029847:CDS
CAGAAGGAGCATTAGGTTTA+GGG 0.264157 1.4:+53248249 None:intergenic
AGTGAAGTGGATAGTGAAAA+AGG 0.270126 1.4:+53248311 None:intergenic
CAAGTAGATTTCAATGGAAT+TGG 0.274341 1.4:-53247241 MS.gene029847:CDS
GGAAGATTTCTCTTCTTCTT+GGG 0.283013 1.4:+53248224 None:intergenic
ACAGAAGGAGCATTAGGTTT+AGG 0.289450 1.4:+53248248 None:intergenic
AAACAACAACTCTATGATTA+TGG 0.293503 1.4:-53247218 MS.gene029847:CDS
GGAGCATTAGGTTTAGGGTT+AGG 0.301624 1.4:+53248254 None:intergenic
TTTGTTGCCATGAGTGTCTT+TGG 0.309658 1.4:+53248062 None:intergenic
ATTATGCACCATGGTGGTTC+TGG 0.314183 1.4:-53246677 MS.gene029847:CDS
ATAAACCAGAAGAACCAAAA+AGG 0.329036 1.4:+53247125 None:intergenic
TGGTTCTGGTTCAAACTCTA+TGG 0.329623 1.4:-53246657 MS.gene029847:CDS
CTATCAAAGCCTGCTTCTTC+CGG 0.333625 1.4:-53246983 MS.gene029847:CDS
GACCTCTTCTATGTAGTTGA+AGG 0.344711 1.4:+53247991 None:intergenic
GAGCATTAGGTTTAGGGTTA+GGG 0.346709 1.4:+53248255 None:intergenic
TTTCTTAATAACATCTTTGT+TGG 0.351397 1.4:+53247938 None:intergenic
CATGGAATACTTCAAAATAT+TGG 0.352848 1.4:-53247304 MS.gene029847:CDS
TCCAATATGCAAATGATTCC+TGG 0.354400 1.4:-53248338 None:intergenic
TTGGGGACAGAAGGAGCATT+AGG 0.361121 1.4:+53248242 None:intergenic
GTCCCATTTCATGATTCCCT+TGG 0.362528 1.4:+53247161 None:intergenic
TTTCCTTGTTGATCACTTTG+TGG 0.363250 1.4:+53246707 None:intergenic
TCTTCTTCATCATCAATGTT+TGG 0.374372 1.4:-53247046 MS.gene029847:CDS
ATGAATATACCTGGTGTTCC+TGG 0.396720 1.4:+53248203 None:intergenic
AAACCAAGGGAATCATGAAA+TGG 0.400103 1.4:-53247164 MS.gene029847:CDS
TTTATCTGAGATAGTTCAAA+TGG 0.400334 1.4:-53247107 MS.gene029847:CDS
CACTATCCACTTCACTTGGT+GGG 0.416726 1.4:-53248304 MS.gene029847:CDS
TATGAGCTTTCCAATCAGAT+TGG 0.425071 1.4:+53247769 None:intergenic
CTCTTCTTCTTGGGGACAGA+AGG 0.425270 1.4:+53248233 None:intergenic
TTCTACTAGTTCAGCTATGT+TGG 0.428171 1.4:-53246807 MS.gene029847:CDS
TCTACTAGTTCAGCTATGTT+GGG 0.430741 1.4:-53246806 MS.gene029847:CDS
TGGTGGACACAAGTCTTCTC+TGG 0.431235 1.4:+53247900 None:intergenic
AGAGCAATAACCTCGGCATC+TGG 0.433782 1.4:+53248089 None:intergenic
TCACTATCCACTTCACTTGG+TGG 0.459561 1.4:-53248305 MS.gene029847:CDS
GATCTCTGTCTTAGCTTCCA+TGG 0.464655 1.4:+53247960 None:intergenic
CTGATTACCTGTGAATTGAA+TGG 0.467069 1.4:+53246526 None:intergenic
AATATGCAGTCCAATCTGAT+TGG 0.470247 1.4:-53247779 MS.gene029847:CDS
AAAGGCCAAGTTTATCACTT+TGG 0.471302 1.4:-53247189 MS.gene029847:CDS
AAGAAGAAGAGAAATCTTCC+AGG 0.472027 1.4:-53248221 MS.gene029847:CDS
GAAGATTTCTCTTCTTCTTG+GGG 0.478434 1.4:+53248225 None:intergenic
TTTCTTGGTGTGAGTGGTGG+AGG 0.481376 1.4:-53246602 MS.gene029847:CDS
GCTTCTTCCATTCAATTCAC+AGG 0.484056 1.4:-53246533 MS.gene029847:CDS
TATCATCAAGTAGATTTCAA+TGG 0.492025 1.4:-53247247 MS.gene029847:CDS
TCTGTCTTAGCTTCCATGGA+AGG 0.492579 1.4:+53247964 None:intergenic
GTGAGTGGTGGAGGAAGAAC+TGG 0.493538 1.4:-53246593 MS.gene029847:CDS
AACCAAGGGAATCATGAAAT+GGG 0.497808 1.4:-53247163 MS.gene029847:CDS
TCTGTGAAATATGCAACAAA+GGG 0.500256 1.4:-53248031 MS.gene029847:CDS
CATCCACAATTTGGTTCACA+TGG 0.504169 1.4:-53247271 MS.gene029847:CDS
AATATTATACCTTGAGAAGA+GGG 0.509866 1.4:+53247704 None:intergenic
ATCTGTGAAATATGCAACAA+AGG 0.510966 1.4:-53248032 MS.gene029847:CDS
AGCAGAAACAGTAACAGAAC+CGG 0.511338 1.4:+53246964 None:intergenic
GAAGAGGTCACAACCTTCCA+TGG 0.514490 1.4:-53247977 MS.gene029847:CDS
TTTAACCAAAGTGATAAACT+TGG 0.528252 1.4:+53247184 None:intergenic
AGATCACCAAGTGCTCTTGA+AGG 0.529586 1.4:+53247873 None:intergenic
GCAGATGTTGAATTTGAAGC+AGG 0.534808 1.4:+53247019 None:intergenic
GGAATACTTCAAAATATTGG+TGG 0.535088 1.4:-53247301 MS.gene029847:CDS
TGATGAAGAAGAACAAATCA+AGG 0.539973 1.4:+53247057 None:intergenic
CATGACCCTTCAAGAGCACT+TGG 0.544018 1.4:-53247879 MS.gene029847:CDS
ATGTTGGGAAATAGTAGTGC+AGG 0.545999 1.4:-53246791 MS.gene029847:CDS
TGTGAACCAAATTGTGGATG+TGG 0.555768 1.4:+53247274 None:intergenic
ACTAAACCCACCAAGTGAAG+TGG 0.557454 1.4:+53248298 None:intergenic
TATCACTTTGGTTAAACCAA+GGG 0.558927 1.4:-53247177 MS.gene029847:CDS
AGAAATCTTCCAGGAACACC+AGG 0.564051 1.4:-53248212 MS.gene029847:intron
CAAGTGCTCTTGAAGGGTCA+TGG 0.570425 1.4:+53247880 None:intergenic
GAAAGCACGGTGAAAAGAAG+TGG 0.572977 1.4:-53247824 MS.gene029847:CDS
GTAACAGAACCGGAAGAAGC+AGG 0.576220 1.4:+53246974 None:intergenic
AAATATTATACCTTGAGAAG+AGG 0.578176 1.4:+53247703 None:intergenic
AACCTTCAACTACATAGAAG+AGG 0.579451 1.4:-53247993 MS.gene029847:CDS
GCTGAACTAGTAGAAGAAGA+AGG 0.581667 1.4:+53246815 None:intergenic
TTATCTGAGATAGTTCAAAT+GGG 0.582881 1.4:-53247106 MS.gene029847:CDS
ACTGTGGCACCCTCTTCTCA+AGG 0.585281 1.4:-53247713 MS.gene029847:intron
GATCACCAAGTGCTCTTGAA+GGG 0.591900 1.4:+53247874 None:intergenic
AGAGCACTTGGTGATCTCAC+AGG 0.592028 1.4:-53247867 MS.gene029847:CDS
TCACAACATGGTTTATCACA+TGG 0.594739 1.4:-53247322 MS.gene029847:CDS
CTATGATTATGGAGCAACAA+AGG 0.599161 1.4:-53247207 MS.gene029847:CDS
TTATCACTTTGGTTAAACCA+AGG 0.601736 1.4:-53247178 MS.gene029847:CDS
GATAAACCATGTTGTGAATG+TGG 0.612705 1.4:+53247328 None:intergenic
TCTTGAGGTAGAAACTGATG+TGG 0.615192 1.4:+53246569 None:intergenic
TATCCATGTGAACCAAATTG+TGG 0.627461 1.4:+53247268 None:intergenic
AAGCACTTTAGTAGAAAGCA+CGG 0.627488 1.4:-53247837 MS.gene029847:CDS
TTGAATATTATGCACCATGG+TGG 0.642049 1.4:-53246683 MS.gene029847:CDS
TCTATCACCAAAGACACTCA+TGG 0.646655 1.4:-53248069 MS.gene029847:CDS
AGAAGAGCAGTAGCTGACAT+TGG 0.651547 1.4:+53246938 None:intergenic
ATGAATCCACATTCACAACA+TGG 0.651933 1.4:-53247334 MS.gene029847:CDS
CTATACAGATCCAGATGCCG+AGG 0.652908 1.4:-53248099 MS.gene029847:intron
GTGCTCTTGAAGGGTCATGG+TGG 0.656702 1.4:+53247883 None:intergenic
AGAGAATACAAATGTGACTG+TGG 0.678918 1.4:-53247729 MS.gene029847:CDS
AACCAGAACCAGAACCACCA+TGG 0.689052 1.4:+53246669 None:intergenic
AAATTGAATATTATGCACCA+TGG 0.694171 1.4:-53246686 MS.gene029847:CDS
TGGTGATAGAGCAATAACCT+CGG 0.716517 1.4:+53248082 None:intergenic
GGACCACAAAGTGATCAACA+AGG 0.742344 1.4:-53246710 MS.gene029847:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTTCATATATTTCAAAT+TGG - chr1.4:53246715-53246734 MS.gene029847:CDS 15.0%
!! CATATATTTCAAATTGGTAA+TGG - chr1.4:53246721-53246740 MS.gene029847:CDS 20.0%
!! GAATTTAATGCTTTCTTTAA+TGG - chr1.4:53247305-53247324 MS.gene029847:CDS 20.0%
!! TTTCTTAATAACATCTTTGT+TGG + chr1.4:53246920-53246939 None:intergenic 20.0%
!!! CTAAATTTTAAGAATGAAGA+AGG - chr1.4:53247488-53247507 MS.gene029847:intron 20.0%
! AAACAACAACTCTATGATTA+TGG - chr1.4:53247637-53247656 MS.gene029847:intron 25.0%
! AAACTAGAACTAAAAATGGA+AGG + chr1.4:53248004-53248023 None:intergenic 25.0%
! AAATATTATACCTTGAGAAG+AGG + chr1.4:53247155-53247174 None:intergenic 25.0%
! AAATTGAATATTATGCACCA+TGG - chr1.4:53248169-53248188 MS.gene029847:intron 25.0%
! AACTAGAACTAAAAATGGAA+GGG + chr1.4:53248003-53248022 None:intergenic 25.0%
! AATATTATACCTTGAGAAGA+GGG + chr1.4:53247154-53247173 None:intergenic 25.0%
! ACAAAAGATTGGTGAATAAA+AGG + chr1.4:53247188-53247207 None:intergenic 25.0%
! ACCAAAACTAGAACTAAAAA+TGG + chr1.4:53248008-53248027 None:intergenic 25.0%
! CATGGAATACTTCAAAATAT+TGG - chr1.4:53247551-53247570 MS.gene029847:intron 25.0%
! TAATCATGAATGAATATACC+TGG + chr1.4:53246664-53246683 None:intergenic 25.0%
! TATCATCAAGTAGATTTCAA+TGG - chr1.4:53247608-53247627 MS.gene029847:intron 25.0%
! TTATCACAGAAACAAAAGAT+TGG + chr1.4:53247199-53247218 None:intergenic 25.0%
! TTTAACCAAAGTGATAAACT+TGG + chr1.4:53247674-53247693 None:intergenic 25.0%
!! AATTTGACAAGAGATTTTCT+TGG - chr1.4:53248238-53248257 MS.gene029847:CDS 25.0%
!! TTATCTGAGATAGTTCAAAT+GGG - chr1.4:53247749-53247768 MS.gene029847:CDS 25.0%
!! TTTATCTGAGATAGTTCAAA+TGG - chr1.4:53247748-53247767 MS.gene029847:CDS 25.0%
!!! GGATTATATTTTTATGTGTC+TGG - chr1.4:53247326-53247345 MS.gene029847:CDS 25.0%
!!! TCCATTTTTAGTTCTAGTTT+TGG - chr1.4:53248004-53248023 MS.gene029847:CDS 25.0%
!!! TGTTGATATTTTGAACAGAA+AGG - chr1.4:53247385-53247404 MS.gene029847:CDS 25.0%
AAAGCTCATACAAAAACATG+TGG - chr1.4:53247099-53247118 MS.gene029847:CDS 30.0%
ATAAACCAGAAGAACCAAAA+AGG + chr1.4:53247733-53247752 None:intergenic 30.0%
ATCTGTGAAATATGCAACAA+AGG - chr1.4:53246823-53246842 MS.gene029847:CDS 30.0%
CAAGTAGATTTCAATGGAAT+TGG - chr1.4:53247614-53247633 MS.gene029847:intron 30.0%
GGAATACTTCAAAATATTGG+TGG - chr1.4:53247554-53247573 MS.gene029847:intron 30.0%
TAAACCAGAAGAACCAAAAA+GGG + chr1.4:53247732-53247751 None:intergenic 30.0%
TATCACTTTGGTTAAACCAA+GGG - chr1.4:53247678-53247697 MS.gene029847:intron 30.0%
TCTGTGAAATATGCAACAAA+GGG - chr1.4:53246824-53246843 MS.gene029847:CDS 30.0%
TCTTCTTCATCATCAATGTT+TGG - chr1.4:53247809-53247828 MS.gene029847:CDS 30.0%
TGATGAAGAAGAACAAATCA+AGG + chr1.4:53247801-53247820 None:intergenic 30.0%
TTATCACTTTGGTTAAACCA+AGG - chr1.4:53247677-53247696 MS.gene029847:intron 30.0%
! TTTGATCAGTTTTTCATGCA+TGG - chr1.4:53248124-53248143 MS.gene029847:intron 30.0%
!! CAAAGTTCTAATTCCCTTTT+TGG - chr1.4:53247716-53247735 MS.gene029847:CDS 30.0%
!!! AGATTGGACTGCATATTTTT+TGG + chr1.4:53247073-53247092 None:intergenic 30.0%
AAACCAAGGGAATCATGAAA+TGG - chr1.4:53247691-53247710 MS.gene029847:intron 35.0%
AAAGGCCAAGTTTATCACTT+TGG - chr1.4:53247666-53247685 MS.gene029847:intron 35.0%
AACCAAGGGAATCATGAAAT+GGG - chr1.4:53247692-53247711 MS.gene029847:intron 35.0%
AACCTTCAACTACATAGAAG+AGG - chr1.4:53246862-53246881 MS.gene029847:CDS 35.0%
AAGAAGAAGAGAAATCTTCC+AGG - chr1.4:53246634-53246653 MS.gene029847:CDS 35.0%
AAGCACTTTAGTAGAAAGCA+CGG - chr1.4:53247018-53247037 MS.gene029847:CDS 35.0%
AATATGCAGTCCAATCTGAT+TGG - chr1.4:53247076-53247095 MS.gene029847:CDS 35.0%
AGAGAATACAAATGTGACTG+TGG - chr1.4:53247126-53247145 MS.gene029847:CDS 35.0%
AGTGAAGTGGATAGTGAAAA+AGG + chr1.4:53246547-53246566 None:intergenic 35.0%
ATGAATCCACATTCACAACA+TGG - chr1.4:53247521-53247540 MS.gene029847:intron 35.0%
CCAATCATGTATGTTGTGTT+AGG - chr1.4:53247278-53247297 MS.gene029847:CDS 35.0%
CCTAACACAACATACATGAT+TGG + chr1.4:53247281-53247300 None:intergenic 35.0%
CTATGATTATGGAGCAACAA+AGG - chr1.4:53247648-53247667 MS.gene029847:intron 35.0%
CTGATTACCTGTGAATTGAA+TGG + chr1.4:53248332-53248351 None:intergenic 35.0%
GAAGATTTCTCTTCTTCTTG+GGG + chr1.4:53246633-53246652 None:intergenic 35.0%
GATAAACCATGTTGTGAATG+TGG + chr1.4:53247530-53247549 None:intergenic 35.0%
GGAAGATTTCTCTTCTTCTT+GGG + chr1.4:53246634-53246653 None:intergenic 35.0%
GTGAAGTGGATAGTGAAAAA+GGG + chr1.4:53246546-53246565 None:intergenic 35.0%
TATCCATGTGAACCAAATTG+TGG + chr1.4:53247590-53247609 None:intergenic 35.0%
TATGAGCTTTCCAATCAGAT+TGG + chr1.4:53247089-53247108 None:intergenic 35.0%
TCACAACATGGTTTATCACA+TGG - chr1.4:53247533-53247552 MS.gene029847:intron 35.0%
TCATATTTGTGACATGTACG+AGG - chr1.4:53247250-53247269 MS.gene029847:CDS 35.0%
TCTACTAGTTCAGCTATGTT+GGG - chr1.4:53248049-53248068 MS.gene029847:CDS 35.0%
TGGAAGATTTCTCTTCTTCT+TGG + chr1.4:53246635-53246654 None:intergenic 35.0%
TTCTACTAGTTCAGCTATGT+TGG - chr1.4:53248048-53248067 MS.gene029847:CDS 35.0%
TTGAATATTATGCACCATGG+TGG - chr1.4:53248172-53248191 MS.gene029847:intron 35.0%
TTTCCTTGTTGATCACTTTG+TGG + chr1.4:53248151-53248170 None:intergenic 35.0%
! TTTTCACTATCCACTTCACT+TGG - chr1.4:53246547-53246566 MS.gene029847:CDS 35.0%
!! GCAAATTTTGCTATCTCTTG+AGG + chr1.4:53248304-53248323 None:intergenic 35.0%
!! GTTGAAGGTTTTGATCTCTT+TGG + chr1.4:53246852-53246871 None:intergenic 35.0%
!!! AATTCCCTTTTTGGTTCTTC+TGG - chr1.4:53247725-53247744 MS.gene029847:CDS 35.0%
AGCAGAAACAGTAACAGAAC+CGG + chr1.4:53247894-53247913 None:intergenic 40.0%
ATGAATATACCTGGTGTTCC+TGG + chr1.4:53246655-53246674 None:intergenic 40.0%
ATGTTGGGAAATAGTAGTGC+AGG - chr1.4:53248064-53248083 MS.gene029847:CDS 40.0%
CTGCAAAAAGCAGCTCAAAT+GGG - chr1.4:53247938-53247957 MS.gene029847:CDS 40.0%
GACCTCTTCTATGTAGTTGA+AGG + chr1.4:53246867-53246886 None:intergenic 40.0%
GCTGAACTAGTAGAAGAAGA+AGG + chr1.4:53248043-53248062 None:intergenic 40.0%
GCTTCTTCCATTCAATTCAC+AGG - chr1.4:53248322-53248341 MS.gene029847:CDS 40.0%
GGAATTCCACATCCACAATT+TGG - chr1.4:53247575-53247594 MS.gene029847:intron 40.0%
TCTATCACCAAAGACACTCA+TGG - chr1.4:53246786-53246805 MS.gene029847:CDS 40.0%
TCTGCAAAAAGCAGCTCAAA+TGG - chr1.4:53247937-53247956 MS.gene029847:CDS 40.0%
TCTTGAGGTAGAAACTGATG+TGG + chr1.4:53248289-53248308 None:intergenic 40.0%
TGGTGATAGAGCAATAACCT+CGG + chr1.4:53246776-53246795 None:intergenic 40.0%
TGTGAACCAAATTGTGGATG+TGG + chr1.4:53247584-53247603 None:intergenic 40.0%
TTTGTTGCCATGAGTGTCTT+TGG + chr1.4:53246796-53246815 None:intergenic 40.0%
! CAGAGCTTTTTGTGATGCAT+TGG - chr1.4:53247424-53247443 MS.gene029847:CDS 40.0%
! CATCCACAATTTGGTTCACA+TGG - chr1.4:53247584-53247603 MS.gene029847:intron 40.0%
! GAGCATTAGGTTTAGGGTTA+GGG + chr1.4:53246603-53246622 None:intergenic 40.0%
! GCAGATGTTGAATTTGAAGC+AGG + chr1.4:53247839-53247858 None:intergenic 40.0%
!! ACAGAAGGAGCATTAGGTTT+AGG + chr1.4:53246610-53246629 None:intergenic 40.0%
!! AGAGATTTTCTTGGTGTGAG+TGG - chr1.4:53248247-53248266 MS.gene029847:CDS 40.0%
!! CAGAAGGAGCATTAGGTTTA+GGG + chr1.4:53246609-53246628 None:intergenic 40.0%
!! TGGTTCTGGTTCAAACTCTA+TGG - chr1.4:53248198-53248217 MS.gene029847:intron 40.0%
ACTAAACCCACCAAGTGAAG+TGG + chr1.4:53246560-53246579 None:intergenic 45.0%
AGAAATCTTCCAGGAACACC+AGG - chr1.4:53246643-53246662 MS.gene029847:CDS 45.0%
AGAAGAGCAGTAGCTGACAT+TGG + chr1.4:53247920-53247939 None:intergenic 45.0%
AGATCACCAAGTGCTCTTGA+AGG + chr1.4:53246985-53247004 None:intergenic 45.0%
ATTATGCACCATGGTGGTTC+TGG - chr1.4:53248178-53248197 MS.gene029847:intron 45.0%
CACTATCCACTTCACTTGGT+GGG - chr1.4:53246551-53246570 MS.gene029847:CDS 45.0%
CTATCAAAGCCTGCTTCTTC+CGG - chr1.4:53247872-53247891 MS.gene029847:CDS 45.0%
GATAGTGAAAAAGGGTCACC+AGG + chr1.4:53246538-53246557 None:intergenic 45.0%
GATCACCAAGTGCTCTTGAA+GGG + chr1.4:53246984-53247003 None:intergenic 45.0%
GATCTCTGTCTTAGCTTCCA+TGG + chr1.4:53246898-53246917 None:intergenic 45.0%
GGACCACAAAGTGATCAACA+AGG - chr1.4:53248145-53248164 MS.gene029847:intron 45.0%
GTCCCATTTCATGATTCCCT+TGG + chr1.4:53247697-53247716 None:intergenic 45.0%
TCACTATCCACTTCACTTGG+TGG - chr1.4:53246550-53246569 MS.gene029847:CDS 45.0%
TCTGTCTTAGCTTCCATGGA+AGG + chr1.4:53246894-53246913 None:intergenic 45.0%
! GAAAGCACGGTGAAAAGAAG+TGG - chr1.4:53247031-53247050 MS.gene029847:CDS 45.0%
! GGAGCATTAGGTTTAGGGTT+AGG + chr1.4:53246604-53246623 None:intergenic 45.0%
!! GATTTTCTTGGTGTGAGTGG+TGG - chr1.4:53248250-53248269 MS.gene029847:CDS 45.0%
AACCAGAACCAGAACCACCA+TGG + chr1.4:53248189-53248208 None:intergenic 50.0%
AGAGCAATAACCTCGGCATC+TGG + chr1.4:53246769-53246788 None:intergenic 50.0%
CAAGTGCTCTTGAAGGGTCA+TGG + chr1.4:53246978-53246997 None:intergenic 50.0%
CTATACAGATCCAGATGCCG+AGG - chr1.4:53246756-53246775 MS.gene029847:CDS 50.0%
CTCTTCTTCTTGGGGACAGA+AGG + chr1.4:53246625-53246644 None:intergenic 50.0%
GAAGAGGTCACAACCTTCCA+TGG - chr1.4:53246878-53246897 MS.gene029847:CDS 50.0%
GTAACAGAACCGGAAGAAGC+AGG + chr1.4:53247884-53247903 None:intergenic 50.0%
! AGAGCACTTGGTGATCTCAC+AGG - chr1.4:53246988-53247007 MS.gene029847:CDS 50.0%
! TGGTGGACACAAGTCTTCTC+TGG + chr1.4:53246958-53246977 None:intergenic 50.0%
! TTTCTTGGTGTGAGTGGTGG+AGG - chr1.4:53248253-53248272 MS.gene029847:CDS 50.0%
!! CATGACCCTTCAAGAGCACT+TGG - chr1.4:53246976-53246995 MS.gene029847:CDS 50.0%
!! TTGGGGACAGAAGGAGCATT+AGG + chr1.4:53246616-53246635 None:intergenic 50.0%
ACTGTGGCACCCTCTTCTCA+AGG - chr1.4:53247142-53247161 MS.gene029847:CDS 55.0%
GTGCTCTTGAAGGGTCATGG+TGG + chr1.4:53246975-53246994 None:intergenic 55.0%
! GTGAGTGGTGGAGGAAGAAC+TGG - chr1.4:53248262-53248281 MS.gene029847:CDS 55.0%
!! CACCATGGTGGTTCTGGTTC+TGG - chr1.4:53248184-53248203 MS.gene029847:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 53246523 53248354 53246523 ID=MS.gene029847
chr1.4 mRNA 53246523 53248354 53246523 ID=MS.gene029847.t1;Parent=MS.gene029847
chr1.4 exon 53248213 53248354 53248213 ID=MS.gene029847.t1.exon1;Parent=MS.gene029847.t1
chr1.4 CDS 53248213 53248354 53248213 ID=cds.MS.gene029847.t1;Parent=MS.gene029847.t1
chr1.4 exon 53247714 53248113 53247714 ID=MS.gene029847.t1.exon2;Parent=MS.gene029847.t1
chr1.4 CDS 53247714 53248113 53247714 ID=cds.MS.gene029847.t1;Parent=MS.gene029847.t1
chr1.4 exon 53246523 53247474 53246523 ID=MS.gene029847.t1.exon3;Parent=MS.gene029847.t1
chr1.4 CDS 53246523 53247474 53246523 ID=cds.MS.gene029847.t1;Parent=MS.gene029847.t1
Gene Sequence

>MS.gene029847

ATGCAAATGATTCCTGGTGACCCTTTTTCACTATCCACTTCACTTGGTGGGTTTAGTACTCAAGAGCAAAACACAAACCCTAACCCTAAACCTAATGCTCCTTCTGTCCCCAAGAAGAAGAGAAATCTTCCAGGAACACCAGGTATATTCATTCATGATTACAGAAATTTTTGTATGATTTTCAATTTGTTACTTTTTCATATATTTCAAATTGGTAATGGAAATTAATTTGACTATACAGATCCAGATGCCGAGGTTATTGCTCTATCACCAAAGACACTCATGGCAACAAACAGATTCATCTGTGAAATATGCAACAAAGGGTTCCAAAGAGATCAAAACCTTCAACTACATAGAAGAGGTCACAACCTTCCATGGAAGCTAAGACAGAGATCCAACAAAGATGTTATTAAGAAAAAAGTTTACATCTGTCCAGAGAAGACTTGTGTCCACCATGACCCTTCAAGAGCACTTGGTGATCTCACAGGTATAAAAAAGCACTTTAGTAGAAAGCACGGTGAAAAGAAGTGGAAATGTGAAAAATGTTCCAAAAAATATGCAGTCCAATCTGATTGGAAAGCTCATACAAAAACATGTGGTACAAGAGAATACAAATGTGACTGTGGCACCCTCTTCTCAAGGTATAATATTTAATATACTCTCCTTTTATTCACCAATCTTTTGTTTCTGTGATAAATCAATAAATGCTTCTTTAATTTATTCATATTCATATTTGTGACATGTACGAGGTTTTTCCAATCATGTATGTTGTGTTAGGTTTTGAATTTAATGCTTTCTTTAATGGATTATATTTTTATGTGTCTGGTTTTAAGTTTTAATTAAGTTTTAATGTCTGTTTTTTTGTTGATATTTTGAACAGAAAGGATAGTTTCATTACACACAGAGCTTTTTGTGATGCATTGGCAGAAGAGAGTGCAAGAGTAACTTCAGTTACAACAACAAATCTAAATTTTAAGAATGAAGAAGGTAGTGCTATGATGAATCCACATTCACAACATGGTTTATCACATGGAATACTTCAAAATATTGGTGGAATTCCACATCCACAATTTGGTTCACATGGATATCATCAAGTAGATTTCAATGGAATTGGAAACAACAACTCTATGATTATGGAGCAACAAAGGCCAAGTTTATCACTTTGGTTAAACCAAGGGAATCATGAAATGGGACAAAGTTCTAATTCCCTTTTTGGTTCTTCTGGTTTATCTGAGATAGTTCAAATGGGAAATGCTACTACTAATAATAATAATGCCTTGATTTGTTCTTCTTCATCATCAATGTTTGGTGTACCTGCTTCAAATTCAACATCTGCTGCAAATCTTTCTCTATCAAAGCCTGCTTCTTCCGGTTCTGTTACTGTTTCTGCTACACCAATGTCAGCTACTGCTCTTCTGCAAAAAGCAGCTCAAATGGGTTCAACTAGAAGCAATACTAGTAATGATAATAATAACAACCCTTCCATTTTTAGTTCTAGTTTTGGTGTAATGACATCACCTTCTTCTTCTACTAGTTCAGCTATGTTGGGAAATAGTAGTGCAGGTGCAGCTGCTAATGCTAATTATAGCTCAAATAGTAGTTTTGATCAGTTTTTCATGCATGGACCACAAAGTGATCAACAAGGAAAATTGAATATTATGCACCATGGTGGTTCTGGTTCTGGTTCAAACTCTATGGATCAAGTACAACATAGTAATTTGACAAGAGATTTTCTTGGTGTGAGTGGTGGAGGAAGAACTGGTCCACATCAGTTTCTACCTCAAGAGATAGCAAAATTTGCTTCTTCCATTCAATTCACAGGTAATCAGTGA
Protein sequence

>MS.gene029847.t1

MQMIPGDPFSLSTSLGGFSTQEQNTNPNPKPNAPSVPKKKRNLPGTPDPDAEVIALSPKTLMATNRFICEICNKGFQRDQNLQLHRRGHNLPWKLRQRSNKDVIKKKVYICPEKTCVHHDPSRALGDLTGIKKHFSRKHGEKKWKCEKCSKKYAVQSDWKAHTKTCGTREYKCDCGTLFSRKDSFITHRAFCDALAEESARVTSVTTTNLNFKNEEGSAMMNPHSQHGLSHGILQNIGGIPHPQFGSHGYHQVDFNGIGNNNSMIMEQQRPSLSLWLNQGNHEMGQSSNSLFGSSGLSEIVQMGNATTNNNNALICSSSSSMFGVPASNSTSAANLSLSKPASSGSVTVSATPMSATALLQKAAQMGSTRSNTSNDNNNNPSIFSSSFGVMTSPSSSTSSAMLGNSSAGAAANANYSSNSSFDQFFMHGPQSDQQGKLNIMHHGGSGSGSNSMDQVQHSNLTRDFLGVSGGGRTGPHQFLPQEIAKFASSIQFTGNQ