AlfalfaGEDB | MS.gene03032

M. sativa cultivar XinJiangDaYe / MS.gene03032

KEGG: K08065
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046982 GO:0046983
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03032.t1	XP_013469195.1	76.8	194	36	2	1	185	1	194	1.60E-69	272.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03032.t1	O82248	78.8	99	21	0	34	132	51	149	1.6e-41	170.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03032.t1	I3TAW4	76.8	194	36	2	1	185	1	194	1.2e-69	272.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene03032.t1	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03032.t1	MTR_1g088860	88.660	194	13	2	1	185	1	194	3.97e-118	333
MS.gene03032.t1	MTR_4g119500	78.095	105	23	0	24	128	12	116	2.55e-61	186
MS.gene03032.t1	MTR_0392s0020	68.992	129	37	1	24	152	8	133	1.24e-60	185
MS.gene03032.t1	MTR_1g083070	74.000	100	26	0	34	133	4	103	5.87e-53	165
MS.gene03032.t1	MTR_4g133938	64.407	118	39	1	35	152	20	134	1.21e-52	165
MS.gene03032.t1	MTR_2g026710	64.407	118	36	1	33	150	16	127	8.52e-52	164
MS.gene03032.t1	MTR_8g093920	72.277	101	28	0	33	133	39	139	1.73e-51	164
MS.gene03032.t1	MTR_7g061270	62.162	111	42	0	32	142	24	134	9.57e-51	161
MS.gene03032.t1	MTR_7g100650	62.162	111	42	0	32	142	24	134	1.54e-50	160
MS.gene03032.t1	MTR_3g058980	60.504	119	47	0	33	151	26	144	1.74e-50	161
MS.gene03032.t1	MTR_5g095740	71.875	96	27	0	33	128	22	117	1.21e-49	159
MS.gene03032.t1	MTR_8g091720	71.429	98	28	0	33	130	27	124	2.80e-49	158
MS.gene03032.t1	MTR_1g072790	60.714	112	44	0	33	144	28	139	7.51e-49	156
MS.gene03032.t1	MTR_4g133952	63.462	104	37	1	26	128	49	152	5.31e-48	156
MS.gene03032.t1	MTR_1g039040	56.604	106	46	0	32	137	4	109	1.55e-42	141
MS.gene03032.t1	MTR_1g029070	44.954	109	60	0	19	127	37	145	2.92e-28	103
MS.gene03032.t1	MTR_5g095900	44.565	92	51	0	31	122	13	104	3.34e-21	86.3
MS.gene03032.t1	MTR_1g029100	40.000	80	48	0	39	118	7	86	2.03e-18	76.3
MS.gene03032.t1	MTR_2g056000	32.727	110	72	1	3	112	12	119	1.49e-17	76.6
MS.gene03032.t1	MTR_1g028480	46.875	64	34	0	64	127	19	82	4.14e-16	70.9
MS.gene03032.t1	MTR_4g112380	32.051	78	53	0	35	112	12	89	3.72e-13	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03032.t1	AT2G47810	77.451	102	23	0	31	132	48	149	4.41e-58	179
MS.gene03032.t1	AT1G09030	57.143	140	56	1	32	171	1	136	8.78e-53	165
MS.gene03032.t1	AT5G47640	68.468	111	32	1	33	140	26	136	1.94e-52	166
MS.gene03032.t1	AT4G14540	75.000	96	24	0	33	128	20	115	5.39e-52	164
MS.gene03032.t1	AT2G13570	73.267	101	27	0	33	133	35	135	2.65e-51	164
MS.gene03032.t1	AT2G38880	64.865	111	38	1	32	141	19	129	2.89e-51	161
MS.gene03032.t1	AT2G38880	64.865	111	38	1	32	141	19	129	3.55e-51	161
MS.gene03032.t1	AT2G38880	64.865	111	38	1	32	141	19	129	3.55e-51	161
MS.gene03032.t1	AT2G38880	64.865	111	38	1	32	141	19	129	3.55e-51	161
MS.gene03032.t1	AT3G53340	50.336	149	67	1	32	180	27	168	5.18e-51	162
MS.gene03032.t1	AT3G53340	50.336	149	67	1	32	180	27	168	5.18e-51	162
MS.gene03032.t1	AT2G38880	65.455	110	37	1	32	141	19	127	3.30e-50	159
MS.gene03032.t1	AT2G37060	63.063	111	41	0	32	142	28	138	4.66e-50	159
MS.gene03032.t1	AT2G37060	63.063	111	41	0	32	142	28	138	4.66e-50	159
MS.gene03032.t1	AT2G37060	63.063	111	41	0	32	142	28	138	4.66e-50	159
MS.gene03032.t1	AT2G38880	73.118	93	25	0	32	124	19	111	2.15e-49	155
MS.gene03032.t1	AT3G53340	72.043	93	26	0	32	124	27	119	6.02e-49	155
MS.gene03032.t1	AT2G38880	73.118	93	25	0	32	124	19	111	6.21e-49	156
MS.gene03032.t1	AT2G38880	73.118	93	25	0	32	124	19	111	6.21e-49	156
MS.gene03032.t1	AT3G53340	69.697	99	29	1	32	130	27	124	7.90e-49	155
MS.gene03032.t1	AT3G53340	69.697	99	29	1	32	130	27	124	7.90e-49	155
MS.gene03032.t1	AT2G38880	73.118	93	25	0	32	124	19	111	9.08e-49	155
MS.gene03032.t1	AT2G38880	73.118	93	25	0	32	124	19	111	9.08e-49	155
MS.gene03032.t1	AT2G38880	73.118	93	25	0	32	124	19	111	9.08e-49	155
MS.gene03032.t1	AT5G47670	63.265	98	36	0	32	129	27	124	5.45e-46	150
MS.gene03032.t1	AT5G47670	62.626	99	37	0	31	129	55	153	1.27e-45	150
MS.gene03032.t1	AT5G47670	62.626	99	37	0	31	129	55	153	1.27e-45	150
MS.gene03032.t1	AT1G21970	50.862	116	57	0	19	134	44	159	1.37e-41	140
MS.gene03032.t1	AT5G23090	35.052	97	61	1	35	129	12	108	1.57e-17	75.9
MS.gene03032.t1	AT5G23090	38.462	78	48	0	35	112	12	89	1.26e-16	73.6
MS.gene03032.t1	AT5G23090	38.462	78	48	0	35	112	12	89	1.26e-16	73.6
MS.gene03032.t1	AT5G23090	38.462	78	48	0	35	112	12	89	1.26e-16	73.6
MS.gene03032.t1	AT5G08190	32.292	96	63	1	35	128	12	107	4.85e-16	72.0
MS.gene03032.t1	AT5G08190	34.615	78	51	0	35	112	12	89	6.92e-15	68.9

Find 35 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATGGTTTCTTTAGCATCTT+TGG	0.164527	1.3:+62189723	None:intergenic
CCTCTTCTATGTTCAATAAT+AGG	0.214525	1.3:-62189393	MS.gene03032:CDS
GATGATGTTTGTTGGGCTTT+GGG	0.221205	1.3:-62189613	MS.gene03032:CDS
TGATGTTGTTACCCTTATTT+TGG	0.229982	1.3:+62189509	None:intergenic
TGATGATGTTTGTTGGGCTT+TGG	0.269661	1.3:-62189614	MS.gene03032:CDS
GGAAAGAGGGAACCAAAATA+AGG	0.312778	1.3:-62189521	MS.gene03032:CDS
TGTTGGGCTTTGGGGACACT+AGG	0.347462	1.3:-62189604	MS.gene03032:CDS
AAAGGTATTTGTATAAGTAT+AGG	0.352771	1.3:-62189555	MS.gene03032:CDS
TTTGTATAAGTATAGGGAAT+TGG	0.359649	1.3:-62189548	MS.gene03032:CDS
GAAGCAACATTCACTTTCCT+TGG	0.401544	1.3:+62189415	None:intergenic
ATCCGACCAACATTAGCTAT+TGG	0.403010	1.3:+62189781	None:intergenic
AAGGTATTTGTATAAGTATA+GGG	0.417767	1.3:-62189554	MS.gene03032:CDS
GAATGGTGATGATGTTTGTT+GGG	0.429389	1.3:-62189620	MS.gene03032:CDS
GAAACATATGATTATGAAGA+TGG	0.453321	1.3:-62189451	MS.gene03032:CDS
ACATGGGTGATAACAACAAA+GGG	0.487792	1.3:-62189900	MS.gene03032:CDS
GAAAGAGGGAACCAAAATAA+GGG	0.501997	1.3:-62189520	MS.gene03032:CDS
GTTGGGCTTTGGGGACACTA+GGG	0.503961	1.3:-62189603	MS.gene03032:CDS
ATGATGTTTGTTGGGCTTTG+GGG	0.505210	1.3:-62189612	MS.gene03032:CDS
TGAATGGTGATGATGTTTGT+TGG	0.510123	1.3:-62189621	MS.gene03032:CDS
CTCTGAGACACACTCTTGCA+TGG	0.513185	1.3:+62189705	None:intergenic
GAGAAGCGCAAGACAGTGAA+TGG	0.520201	1.3:-62189637	MS.gene03032:CDS
AGGGAATTGGATGCGGAAAG+AGG	0.523773	1.3:-62189535	MS.gene03032:CDS
TTGCTTCCAATAGCTAATGT+TGG	0.538809	1.3:-62189787	MS.gene03032:CDS
TTCAAAATCCCCTTGACTGA+AGG	0.540445	1.3:+62189362	None:intergenic
GGGAATTGGATGCGGAAAGA+GGG	0.541973	1.3:-62189534	MS.gene03032:CDS
CCTATTATTGAACATAGAAG+AGG	0.550260	1.3:+62189393	None:intergenic
TAAGTATAGGGAATTGGATG+CGG	0.557603	1.3:-62189542	MS.gene03032:CDS
ATTACGCTGAGCCACTCAAA+AGG	0.563009	1.3:-62189573	MS.gene03032:CDS
GAACAGTGACCTTCAGTCAA+GGG	0.574883	1.3:-62189371	MS.gene03032:CDS
TTCCAATAGCTAATGTTGGT+CGG	0.585707	1.3:-62189783	MS.gene03032:CDS
ATGGTAAGTCACAAACTCCA+AGG	0.613914	1.3:-62189432	MS.gene03032:CDS
AACAGTGACCTTCAGTCAAG+GGG	0.614235	1.3:-62189370	MS.gene03032:CDS
AACATGGGTGATAACAACAA+AGG	0.619511	1.3:-62189901	MS.gene03032:CDS
GGAACAGTGACCTTCAGTCA+AGG	0.622121	1.3:-62189372	MS.gene03032:CDS
AGCATCTGACAAGTGTCACA+AGG	0.642405	1.3:-62189659	MS.gene03032:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAGGTATTTGTATAAGTAT+AGG	-	chr1.3:62189706-62189725	MS.gene03032:CDS	20.0%
!!!	AAGGTATTTGTATAAGTATA+GGG	-	chr1.3:62189707-62189726	MS.gene03032:CDS	20.0%
!	GAAACATATGATTATGAAGA+TGG	-	chr1.3:62189810-62189829	MS.gene03032:CDS	25.0%
!	TTTGTATAAGTATAGGGAAT+TGG	-	chr1.3:62189713-62189732	MS.gene03032:CDS	25.0%
!!	TTATACAAATACCTTTTGAG+TGG	+	chr1.3:62189702-62189721	None:intergenic	25.0%
	CCTATTATTGAACATAGAAG+AGG	+	chr1.3:62189871-62189890	None:intergenic	30.0%
	CCTCTTCTATGTTCAATAAT+AGG	-	chr1.3:62189868-62189887	MS.gene03032:CDS	30.0%
!!	TCTTTGGAGATTTTTGCATT+TGG	+	chr1.3:62189525-62189544	None:intergenic	30.0%
!!	TGATGTTGTTACCCTTATTT+TGG	+	chr1.3:62189755-62189774	None:intergenic	30.0%
!!!	GAGTTTGTTAGTTTTGTAAC+TGG	-	chr1.3:62189576-62189595	MS.gene03032:CDS	30.0%
	CATGGTTTCTTTAGCATCTT+TGG	+	chr1.3:62189541-62189560	None:intergenic	35.0%
	GAAAGAGGGAACCAAAATAA+GGG	-	chr1.3:62189741-62189760	MS.gene03032:CDS	35.0%
	TAAGTATAGGGAATTGGATG+CGG	-	chr1.3:62189719-62189738	MS.gene03032:CDS	35.0%
	TTCCAATAGCTAATGTTGGT+CGG	-	chr1.3:62189478-62189497	MS.gene03032:CDS	35.0%
	TTGCTTCCAATAGCTAATGT+TGG	-	chr1.3:62189474-62189493	MS.gene03032:CDS	35.0%
!	GAATGGTGATGATGTTTGTT+GGG	-	chr1.3:62189641-62189660	MS.gene03032:CDS	35.0%
!	TGAATGGTGATGATGTTTGT+TGG	-	chr1.3:62189640-62189659	MS.gene03032:CDS	35.0%
!	TTGGAGATTTTTGCATTTGG+TGG	+	chr1.3:62189522-62189541	None:intergenic	35.0%
	ATCCGACCAACATTAGCTAT+TGG	+	chr1.3:62189483-62189502	None:intergenic	40.0%
	ATGGTAAGTCACAAACTCCA+AGG	-	chr1.3:62189829-62189848	MS.gene03032:CDS	40.0%
	GAAGCAACATTCACTTTCCT+TGG	+	chr1.3:62189849-62189868	None:intergenic	40.0%
	GGAAAGAGGGAACCAAAATA+AGG	-	chr1.3:62189740-62189759	MS.gene03032:CDS	40.0%
!	ATGATGTTTGTTGGGCTTTG+GGG	-	chr1.3:62189649-62189668	MS.gene03032:CDS	40.0%
!	GATGATGTTTGTTGGGCTTT+GGG	-	chr1.3:62189648-62189667	MS.gene03032:CDS	40.0%
!	TGATGATGTTTGTTGGGCTT+TGG	-	chr1.3:62189647-62189666	MS.gene03032:CDS	40.0%
	AACAGTGACCTTCAGTCAAG+GGG	-	chr1.3:62189891-62189910	MS.gene03032:CDS	45.0%
	AGCATCTGACAAGTGTCACA+AGG	-	chr1.3:62189602-62189621	MS.gene03032:CDS	45.0%
	ATTACGCTGAGCCACTCAAA+AGG	-	chr1.3:62189688-62189707	MS.gene03032:CDS	45.0%
	GAACAGTGACCTTCAGTCAA+GGG	-	chr1.3:62189890-62189909	MS.gene03032:CDS	45.0%
	AGGGAATTGGATGCGGAAAG+AGG	-	chr1.3:62189726-62189745	MS.gene03032:CDS	50.0%
	GGAACAGTGACCTTCAGTCA+AGG	-	chr1.3:62189889-62189908	MS.gene03032:CDS	50.0%
	GGGAATTGGATGCGGAAAGA+GGG	-	chr1.3:62189727-62189746	MS.gene03032:CDS	50.0%
!	GAGAAGCGCAAGACAGTGAA+TGG	-	chr1.3:62189624-62189643	MS.gene03032:CDS	50.0%
!!	CTCTGAGACACACTCTTGCA+TGG	+	chr1.3:62189559-62189578	None:intergenic	50.0%
!!	GTTGGGCTTTGGGGACACTA+GGG	-	chr1.3:62189658-62189677	MS.gene03032:CDS	55.0%
!!	TGTTGGGCTTTGGGGACACT+AGG	-	chr1.3:62189657-62189676	MS.gene03032:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	62189363	62189920	62189363	ID=MS.gene03032
chr1.3	mRNA	62189363	62189920	62189363	ID=MS.gene03032.t1;Parent=MS.gene03032
chr1.3	exon	62189363	62189920	62189363	ID=MS.gene03032.t1.exon1;Parent=MS.gene03032.t1
chr1.3	CDS	62189363	62189920	62189363	ID=cds.MS.gene03032.t1;Parent=MS.gene03032.t1

Gene Sequence

>MS.gene03032

ATGGGTGATAACAACAAAGGGAAACTAGTCTCAGATAGAGAAGTCTTTAAGTACAACACAAATCTCACATCTCATGAGCAAGATCATGAAGTAATCAAAGAGCAAGATCGTTTGCTTCCAATAGCTAATGTTGGTCGGATCATGAAACAAATCTTGCCACCAAATGCAAAAATCTCCAAAGATGCTAAAGAAACCATGCAAGAGTGTGTCTCAGAGTTTGTTAGTTTTGTAACTGGTGAAGCATCTGACAAGTGTCACAAGGAGAAGCGCAAGACAGTGAATGGTGATGATGTTTGTTGGGCTTTGGGGACACTAGGGTTTGATGATTACGCTGAGCCACTCAAAAGGTATTTGTATAAGTATAGGGAATTGGATGCGGAAAGAGGGAACCAAAATAAGGGTAACAACATCAACAACAACAGTGATGAAATTGAAAACAATATTAATGAAACATATGATTATGAAGATGGTAAGTCACAAACTCCAAGGAAAGTGAATGTTGCTTCCTCTTCTATGTTCAATAATAGGAACAGTGACCTTCAGTCAAGGGGATTTTGA

Protein sequence

>MS.gene03032.t1

MGDNNKGKLVSDREVFKYNTNLTSHEQDHEVIKEQDRLLPIANVGRIMKQILPPNAKISKDAKETMQECVSEFVSFVTGEASDKCHKEKRKTVNGDDVCWALGTLGFDDYAEPLKRYLYKYRELDAERGNQNKGNNINNNSDEIENNINETYDYEDGKSQTPRKVNVASSSMFNNRNSDLQSRGF