AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030352

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030352.t1 MTR_8g051580 95.536 112 5 0 1 112 31 142 1.62e-73 216
MS.gene030352.t1 MTR_8g046350 59.821 112 39 2 1 112 31 136 4.77e-38 125
MS.gene030352.t1 MTR_1g047550 50.893 112 55 0 1 112 31 142 7.82e-37 123
MS.gene030352.t1 MTR_1g063160 53.774 106 49 0 1 106 31 136 1.38e-36 122
MS.gene030352.t1 MTR_8g079502 50.000 112 56 0 1 112 31 142 2.28e-35 119
MS.gene030352.t1 MTR_1g054265 50.450 111 55 0 1 111 31 141 2.19e-33 114
MS.gene030352.t1 MTR_4g131030 42.636 129 57 1 1 112 31 159 3.76e-31 110
MS.gene030352.t1 MTR_3g080940 34.848 132 59 4 1 109 38 165 9.20e-15 67.4
MS.gene030352.t1 MTR_1g106070 41.667 72 40 1 1 70 38 109 1.08e-13 63.9
MS.gene030352.t1 MTR_3g052870 42.857 77 32 2 1 70 38 109 1.99e-13 63.9
MS.gene030352.t1 MTR_1g105920 42.857 77 32 2 1 70 38 109 2.07e-13 64.3
MS.gene030352.t1 MTR_1g108580 39.175 97 54 2 1 95 38 131 3.58e-13 63.5
MS.gene030352.t1 MTR_5g045560 40.260 77 44 1 1 77 38 112 9.03e-13 62.4
MS.gene030352.t1 MTR_4g094632 38.462 78 48 0 1 78 31 108 1.44e-12 60.5
MS.gene030352.t1 MTR_1g105910 41.558 77 33 2 1 70 38 109 2.60e-12 61.2
MS.gene030352.t1 MTR_3g052920 41.892 74 31 2 1 67 38 106 3.30e-12 60.8
MS.gene030352.t1 MTR_1g105905 37.113 97 56 2 1 95 21 114 6.68e-12 59.7
MS.gene030352.t1 MTR_0121s0080 57.143 42 18 0 1 42 38 79 9.18e-12 59.7
MS.gene030352.t1 MTR_3g030770 36.170 94 44 3 1 78 38 131 1.12e-11 59.3
MS.gene030352.t1 MTR_7g062350 57.143 42 18 0 1 42 50 91 1.43e-11 59.3
MS.gene030352.t1 MTR_2g030740 45.902 61 26 1 1 54 38 98 1.88e-11 58.9
MS.gene030352.t1 MTR_8g043650 40.278 72 41 1 1 70 38 109 3.12e-11 58.2
MS.gene030352.t1 MTR_1g108510 36.170 94 44 3 1 78 38 131 3.46e-11 58.2
MS.gene030352.t1 MTR_1g012570 38.961 77 35 2 1 70 38 109 4.37e-11 57.8
MS.gene030352.t1 MTR_7g028448 38.333 60 37 0 1 60 41 100 6.97e-11 56.2
MS.gene030352.t1 MTR_4g036915 38.333 60 37 0 1 60 41 100 6.97e-11 56.2
MS.gene030352.t1 MTR_2g105290 37.681 69 41 1 1 69 41 107 8.42e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030352.t1 AT2G24840 41.758 91 51 2 1 90 92 181 2.85e-17 74.7
MS.gene030352.t1 AT5G60440 42.708 96 38 3 1 87 36 123 8.87e-16 71.2
MS.gene030352.t1 AT3G66656 39.785 93 54 1 1 91 31 123 7.64e-15 67.0
MS.gene030352.t1 AT2G34440 46.053 76 33 2 1 68 31 106 3.59e-14 65.1
MS.gene030352.t1 AT1G01530 43.373 83 44 3 1 82 36 116 2.59e-11 58.5
MS.gene030352.t1 AT4G36590 38.776 98 50 4 1 90 36 131 4.38e-11 58.2
MS.gene030352.t1 AT1G29962 41.748 103 51 3 1 102 37 131 4.88e-11 57.0

Find 29 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCTCTCCTTCAAGAAATT+TGG 0.179978 8.1:-56247908 None:intergenic
CAGTCAGTTTGTATTCTTCT+AGG 0.201316 8.1:-56248059 None:intergenic
GGAATAAGCCTTACTCTTTC+GGG 0.222535 8.1:+56247860 MS.gene030352:CDS
AGATTCATATGGGAAATTTA+AGG 0.261798 8.1:+56248107 MS.gene030352:CDS
GGGAATAAGCCTTACTCTTT+CGG 0.264231 8.1:+56247859 MS.gene030352:CDS
ATATGGGAAATTTAAGGAAA+TGG 0.366214 8.1:+56248113 MS.gene030352:CDS
GCTATTGTTGTGTTCTCCCC+TGG 0.368488 8.1:+56247838 MS.gene030352:CDS
GAGGAAATGAAAGATTCATA+TGG 0.425146 8.1:+56248096 MS.gene030352:CDS
CTATTGTTGTGTTCTCCCCT+GGG 0.431123 8.1:+56247839 MS.gene030352:CDS
GTTGCATCCAAATTTCTTGA+AGG 0.449324 8.1:+56247901 MS.gene030352:CDS
ATGAACTTGCCATCTTGTGC+GGG 0.468340 8.1:+56247806 MS.gene030352:CDS
AGCTTCTTATGCCATCATCT+AGG 0.477849 8.1:-56247933 None:intergenic
CTTGGGTGCCCGAAAGAGTA+AGG 0.491836 8.1:-56247868 None:intergenic
TAGGATCTCATCATGTGCCC+TGG 0.515236 8.1:-56248040 None:intergenic
TGTGCTAGCTGAACTAAAAG+AGG 0.527386 8.1:+56248005 MS.gene030352:CDS
AAAGAGTAAGGCTTATTCCC+AGG 0.528202 8.1:-56247856 None:intergenic
AGGAAATGAAAGATTCATAT+GGG 0.533599 8.1:+56248097 MS.gene030352:CDS
AAGAGGCTGAAAATGAAGCC+AGG 0.537040 8.1:+56248022 MS.gene030352:CDS
AATGAACTTGCCATCTTGTG+CGG 0.538350 8.1:+56247805 MS.gene030352:CDS
GATGCAACATCATCAACACT+TGG 0.549337 8.1:-56247886 None:intergenic
AATTTCTGCCCCGCACAAGA+TGG 0.555860 8.1:-56247815 None:intergenic
GGAGAGCTCAACCTAGATGA+TGG 0.566220 8.1:+56247922 MS.gene030352:CDS
GACTGAACTCACTCAACTTG+AGG 0.582374 8.1:+56248077 MS.gene030352:CDS
AAGAGTAAGGCTTATTCCCA+GGG 0.598555 8.1:-56247855 None:intergenic
AGAGGCTGAAAATGAAGCCA+GGG 0.612352 8.1:+56248023 MS.gene030352:CDS
ATGCAACATCATCAACACTT+GGG 0.663273 8.1:-56247885 None:intergenic
TGAACTTGCCATCTTGTGCG+GGG 0.674850 8.1:+56247807 MS.gene030352:CDS
TTCTGAAGTTTCGAACAGAG+AGG 0.689485 8.1:+56247960 MS.gene030352:CDS
AGAGTAAGGCTTATTCCCAG+GGG 0.760036 8.1:-56247854 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGATTCATATGGGAAATTTA+AGG + chr8.1:56248107-56248126 MS.gene030352:CDS 25.0%
! AGGAAATGAAAGATTCATAT+GGG + chr8.1:56248097-56248116 MS.gene030352:CDS 25.0%
GAGGAAATGAAAGATTCATA+TGG + chr8.1:56248096-56248115 MS.gene030352:CDS 30.0%
ATGCAACATCATCAACACTT+GGG - chr8.1:56247888-56247907 None:intergenic 35.0%
CAGTCAGTTTGTATTCTTCT+AGG - chr8.1:56248062-56248081 None:intergenic 35.0%
GTTGCATCCAAATTTCTTGA+AGG + chr8.1:56247901-56247920 MS.gene030352:CDS 35.0%
AAAGAGTAAGGCTTATTCCC+AGG - chr8.1:56247859-56247878 None:intergenic 40.0%
AAGAGTAAGGCTTATTCCCA+GGG - chr8.1:56247858-56247877 None:intergenic 40.0%
AATGAACTTGCCATCTTGTG+CGG + chr8.1:56247805-56247824 MS.gene030352:CDS 40.0%
AGCTTCTTATGCCATCATCT+AGG - chr8.1:56247936-56247955 None:intergenic 40.0%
GAGCTCTCCTTCAAGAAATT+TGG - chr8.1:56247911-56247930 None:intergenic 40.0%
GATGCAACATCATCAACACT+TGG - chr8.1:56247889-56247908 None:intergenic 40.0%
GGAATAAGCCTTACTCTTTC+GGG + chr8.1:56247860-56247879 MS.gene030352:CDS 40.0%
GGGAATAAGCCTTACTCTTT+CGG + chr8.1:56247859-56247878 MS.gene030352:CDS 40.0%
TGTGCTAGCTGAACTAAAAG+AGG + chr8.1:56248005-56248024 MS.gene030352:CDS 40.0%
!! TTCTGAAGTTTCGAACAGAG+AGG + chr8.1:56247960-56247979 MS.gene030352:CDS 40.0%
AAGAGGCTGAAAATGAAGCC+AGG + chr8.1:56248022-56248041 MS.gene030352:CDS 45.0%
AGAGGCTGAAAATGAAGCCA+GGG + chr8.1:56248023-56248042 MS.gene030352:CDS 45.0%
AGAGTAAGGCTTATTCCCAG+GGG - chr8.1:56247857-56247876 None:intergenic 45.0%
ATGAACTTGCCATCTTGTGC+GGG + chr8.1:56247806-56247825 MS.gene030352:CDS 45.0%
CTATTGTTGTGTTCTCCCCT+GGG + chr8.1:56247839-56247858 MS.gene030352:CDS 45.0%
GACTGAACTCACTCAACTTG+AGG + chr8.1:56248077-56248096 MS.gene030352:CDS 45.0%
AATTTCTGCCCCGCACAAGA+TGG - chr8.1:56247818-56247837 None:intergenic 50.0%
GCTATTGTTGTGTTCTCCCC+TGG + chr8.1:56247838-56247857 MS.gene030352:CDS 50.0%
GGAGAGCTCAACCTAGATGA+TGG + chr8.1:56247922-56247941 MS.gene030352:CDS 50.0%
TAGGATCTCATCATGTGCCC+TGG - chr8.1:56248043-56248062 None:intergenic 50.0%
TGAACTTGCCATCTTGTGCG+GGG + chr8.1:56247807-56247826 MS.gene030352:CDS 50.0%
CTTGGGTGCCCGAAAGAGTA+AGG - chr8.1:56247871-56247890 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 56247799 56248134 56247799 ID=MS.gene030352
chr8.1 mRNA 56247799 56248134 56247799 ID=MS.gene030352.t1;Parent=MS.gene030352
chr8.1 exon 56247799 56248134 56247799 ID=MS.gene030352.t1.exon1;Parent=MS.gene030352.t1
chr8.1 CDS 56247799 56248134 56247799 ID=cds.MS.gene030352.t1;Parent=MS.gene030352.t1
Gene Sequence

>MS.gene030352

AAGGCAAATGAACTTGCCATCTTGTGCGGGGCAGAAATTGCTATTGTTGTGTTCTCCCCTGGGAATAAGCCTTACTCTTTCGGGCACCCAAGTGTTGATGATGTTGCATCCAAATTTCTTGAAGGAGAGCTCAACCTAGATGATGGCATAAGAAGCTCATCTTCTGAAGTTTCGAACAGAGAGGATCTGAATCAACAACTTGAAGATGTGCTAGCTGAACTAAAAGAGGCTGAAAATGAAGCCAGGGCACATGATGAGATCCTAGAAGAATACAAACTGACTGAACTCACTCAACTTGAGGAAATGAAAGATTCATATGGGAAATTTAAGGAAATG
Protein sequence

>MS.gene030352.t1

KANELAILCGAEIAIVVFSPGNKPYSFGHPSVDDVASKFLEGELNLDDGIRSSSSEVSNREDLNQQLEDVLAELKEAENEARAHDEILEEYKLTELTQLEEMKDSYGKFKEM