Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030359.t1 | XP_013447077.1 | 88.5 | 182 | 21 | 0 | 1 | 182 | 143 | 324 | 2.30E-84 | 321.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030359.t1 | A0A072TU95 | 88.5 | 182 | 21 | 0 | 1 | 182 | 143 | 324 | 1.7e-84 | 321.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene030359.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030359.t1 | MTR_8g098725 | 98.352 | 182 | 3 | 0 | 1 | 182 | 143 | 324 | 7.52e-134 | 377 |
| MS.gene030359.t1 | MTR_8g098725 | 96.237 | 186 | 3 | 1 | 1 | 182 | 143 | 328 | 8.86e-132 | 372 |
| MS.gene030359.t1 | MTR_5g010120 | 57.419 | 155 | 54 | 3 | 1 | 149 | 92 | 240 | 4.52e-52 | 167 |
| MS.gene030359.t1 | MTR_2g096080 | 49.315 | 146 | 55 | 6 | 1 | 137 | 71 | 206 | 1.21e-28 | 107 |
| MS.gene030359.t1 | MTR_2g068730 | 77.419 | 62 | 14 | 0 | 69 | 130 | 149 | 210 | 9.71e-25 | 97.4 |
| MS.gene030359.t1 | MTR_4g061823 | 47.826 | 138 | 53 | 7 | 1 | 127 | 60 | 189 | 1.53e-23 | 93.6 |
| MS.gene030359.t1 | MTR_4g061910 | 47.826 | 138 | 53 | 7 | 1 | 127 | 60 | 189 | 1.53e-23 | 93.6 |
| MS.gene030359.t1 | MTR_1g073350 | 50.000 | 96 | 37 | 1 | 43 | 127 | 265 | 360 | 1.11e-22 | 94.0 |
| MS.gene030359.t1 | MTR_1g044785 | 48.544 | 103 | 47 | 2 | 69 | 171 | 120 | 216 | 1.69e-22 | 90.5 |
| MS.gene030359.t1 | MTR_1g008220 | 49.412 | 85 | 41 | 1 | 43 | 127 | 194 | 276 | 8.23e-22 | 89.7 |
| MS.gene030359.t1 | MTR_3g091340 | 50.588 | 85 | 40 | 1 | 43 | 127 | 163 | 245 | 3.11e-20 | 85.1 |
| MS.gene030359.t1 | MTR_3g091340 | 50.588 | 85 | 40 | 1 | 43 | 127 | 156 | 238 | 3.44e-20 | 84.7 |
| MS.gene030359.t1 | MTR_3g091340 | 50.588 | 85 | 40 | 1 | 43 | 127 | 192 | 274 | 5.78e-20 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030359.t1 | AT5G41380 | 56.425 | 179 | 56 | 7 | 1 | 167 | 128 | 296 | 2.43e-48 | 159 |
| MS.gene030359.t1 | AT1G63820 | 51.412 | 177 | 55 | 5 | 27 | 172 | 117 | 293 | 1.85e-43 | 146 |
| MS.gene030359.t1 | AT3G12890 | 67.647 | 68 | 22 | 0 | 63 | 130 | 119 | 186 | 4.45e-24 | 95.1 |
| MS.gene030359.t1 | AT2G33350 | 63.768 | 69 | 24 | 1 | 60 | 127 | 286 | 354 | 1.59e-22 | 93.6 |
| MS.gene030359.t1 | AT2G33350 | 63.768 | 69 | 24 | 1 | 60 | 127 | 287 | 355 | 1.62e-22 | 93.6 |
| MS.gene030359.t1 | AT2G33350 | 63.768 | 69 | 24 | 1 | 60 | 127 | 295 | 363 | 2.05e-22 | 93.2 |
| MS.gene030359.t1 | AT2G33350 | 63.768 | 69 | 24 | 1 | 60 | 127 | 275 | 343 | 2.13e-22 | 92.8 |
| MS.gene030359.t1 | AT1G04500 | 68.254 | 63 | 20 | 0 | 74 | 136 | 279 | 341 | 6.74e-22 | 91.7 |
| MS.gene030359.t1 | AT1G04500 | 68.254 | 63 | 20 | 0 | 74 | 136 | 279 | 341 | 6.74e-22 | 91.7 |
| MS.gene030359.t1 | AT1G04500 | 68.254 | 63 | 20 | 0 | 74 | 136 | 270 | 332 | 8.46e-22 | 91.3 |
| MS.gene030359.t1 | AT5G59990 | 42.208 | 154 | 71 | 4 | 1 | 151 | 93 | 231 | 2.53e-20 | 85.1 |
| MS.gene030359.t1 | AT4G27900 | 43.529 | 85 | 44 | 1 | 43 | 127 | 178 | 258 | 4.98e-18 | 79.3 |
| MS.gene030359.t1 | AT4G27900 | 43.529 | 85 | 44 | 1 | 43 | 127 | 178 | 258 | 4.98e-18 | 79.3 |
| MS.gene030359.t1 | AT5G53420 | 43.529 | 85 | 41 | 1 | 43 | 127 | 106 | 183 | 5.06e-17 | 75.1 |
| MS.gene030359.t1 | AT5G53420 | 43.529 | 85 | 41 | 1 | 43 | 127 | 106 | 183 | 5.06e-17 | 75.1 |
| MS.gene030359.t1 | AT5G53420 | 43.529 | 85 | 41 | 1 | 43 | 127 | 106 | 183 | 5.06e-17 | 75.1 |
| MS.gene030359.t1 | AT5G53420 | 43.529 | 85 | 41 | 1 | 43 | 127 | 185 | 262 | 9.31e-17 | 75.9 |
Find 38 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAAGATGAAGTCAATTTC+TGG | 0.241953 | 4.4:+5500928 | MS.gene030359:CDS |
| GAACTTTAACAAGACTATTA+AGG | 0.296751 | 4.4:+5500711 | MS.gene030359:CDS |
| TGTTCTCTTGGCTCTGTACT+TGG | 0.332786 | 4.4:-5500683 | None:intergenic |
| AAAGTTCCTCTGTGTTCTCT+TGG | 0.348760 | 4.4:-5500695 | None:intergenic |
| TTGTAGCACAGGTGATTTGC+AGG | 0.358853 | 4.4:+5500466 | MS.gene030359:CDS |
| GTTGGTCAATTTCAGTACTT+TGG | 0.368744 | 4.4:+5501390 | MS.gene030359:CDS |
| CTGATTAAACATGGAGCCTT+AGG | 0.397252 | 4.4:-5500903 | None:intergenic |
| TTGGTCAATTTCAGTACTTT+GGG | 0.401778 | 4.4:+5501391 | MS.gene030359:CDS |
| TTGAGAAGGGTTTGTAGCAC+AGG | 0.409705 | 4.4:+5500455 | MS.gene030359:CDS |
| TGAAGATGAAGTCAATTTCT+GGG | 0.476045 | 4.4:+5500929 | MS.gene030359:CDS |
| AAGAATAGTGGTTACGGAGT+TGG | 0.483748 | 4.4:+5501372 | MS.gene030359:CDS |
| TCTTGCGAATTCCCCTCCGT+TGG | 0.487308 | 4.4:-5500604 | None:intergenic |
| GAACAATATCTGAAGAATAG+TGG | 0.497442 | 4.4:+5501360 | MS.gene030359:CDS |
| GAGGAACAAAATTTCAAAGT+AGG | 0.499993 | 4.4:+5500625 | MS.gene030359:CDS |
| TGAGTTTGAAATAGAAAACT+AGG | 0.503168 | 4.4:-5500368 | None:intergenic |
| ATCGACCACGCGTACGTGGC+AGG | 0.512738 | 4.4:+5500850 | MS.gene030359:CDS |
| TTGATTCATCGAAATTTCAA+AGG | 0.515625 | 4.4:+5500405 | MS.gene030359:CDS |
| TATCTGAAGAATAGTGGTTA+CGG | 0.515829 | 4.4:+5501366 | MS.gene030359:CDS |
| CCCTGAAAATAGTTTGAGAA+GGG | 0.527305 | 4.4:+5500442 | MS.gene030359:CDS |
| AATAATATGTCTCCAACGGA+GGG | 0.534384 | 4.4:+5500592 | MS.gene030359:CDS |
| ATGAATCAACGAGATTATCA+TGG | 0.556874 | 4.4:-5500391 | None:intergenic |
| AAATAATATGTCTCCAACGG+AGG | 0.560154 | 4.4:+5500591 | MS.gene030359:CDS |
| AGAAAATAATATGTCTCCAA+CGG | 0.563673 | 4.4:+5500588 | MS.gene030359:CDS |
| CGAGCCCACGGAGATTCCTA+AGG | 0.567771 | 4.4:+5500887 | MS.gene030359:CDS |
| TCTTCATCTCTGATTAAACA+TGG | 0.569603 | 4.4:-5500912 | None:intergenic |
| CCCCTGAAAATAGTTTGAGA+AGG | 0.571206 | 4.4:+5500441 | MS.gene030359:CDS |
| TGCACGCAATGACGAGCCCA+CGG | 0.603558 | 4.4:+5500875 | MS.gene030359:CDS |
| GCAAACCTGCCACGTACGCG+TGG | 0.603581 | 4.4:-5500855 | None:intergenic |
| GACAATCGACCACGCGTACG+TGG | 0.616500 | 4.4:+5500846 | MS.gene030359:CDS |
| TGATGTAGATGGAAGAATTG+CGG | 0.651165 | 4.4:+5501304 | MS.gene030359:intron |
| AACGGAGGGGAATTCGCAAG+AGG | 0.653430 | 4.4:+5500606 | MS.gene030359:CDS |
| ATAATATGTCTCCAACGGAG+GGG | 0.654626 | 4.4:+5500593 | MS.gene030359:CDS |
| CAAAGTAGGTCGTTATAGCG+CGG | 0.657198 | 4.4:+5500639 | MS.gene030359:CDS |
| CATGAAGATGATGTAACAGT+GGG | 0.675111 | 4.4:+5501330 | MS.gene030359:CDS |
| GGAGCCTTAGGAATCTCCGT+GGG | 0.684622 | 4.4:-5500891 | None:intergenic |
| GCATGAAGATGATGTAACAG+TGG | 0.695685 | 4.4:+5501329 | MS.gene030359:CDS |
| TGGAGCCTTAGGAATCTCCG+TGG | 0.702219 | 4.4:-5500892 | None:intergenic |
| ACAGAGCCAAGAGAACACAG+AGG | 0.782868 | 4.4:+5500689 | MS.gene030359:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTTTATTGAAGTTTGTTT+TGG | + | chr4.4:5500545-5500564 | MS.gene030359:intron | 20.0% |
| ! | AGAAAATAATATGTCTCCAA+CGG | + | chr4.4:5500588-5500607 | MS.gene030359:CDS | 25.0% |
| ! | GAACTTTAACAAGACTATTA+AGG | + | chr4.4:5500711-5500730 | MS.gene030359:CDS | 25.0% |
| ! | TTGATTCATCGAAATTTCAA+AGG | + | chr4.4:5500405-5500424 | MS.gene030359:CDS | 25.0% |
| !! | TGAGTTTGAAATAGAAAACT+AGG | - | chr4.4:5500371-5500390 | None:intergenic | 25.0% |
| !! | TTTGAAATAGTAAGCATGTA+AGG | + | chr4.4:5501015-5501034 | MS.gene030359:intron | 25.0% |
| ATGAAGATGAAGTCAATTTC+TGG | + | chr4.4:5500928-5500947 | MS.gene030359:CDS | 30.0% | |
| ATGAATCAACGAGATTATCA+TGG | - | chr4.4:5500394-5500413 | None:intergenic | 30.0% | |
| GAACAATATCTGAAGAATAG+TGG | + | chr4.4:5501360-5501379 | MS.gene030359:CDS | 30.0% | |
| GAGGAACAAAATTTCAAAGT+AGG | + | chr4.4:5500625-5500644 | MS.gene030359:CDS | 30.0% | |
| TATCTGAAGAATAGTGGTTA+CGG | + | chr4.4:5501366-5501385 | MS.gene030359:CDS | 30.0% | |
| TCTTCATCTCTGATTAAACA+TGG | - | chr4.4:5500915-5500934 | None:intergenic | 30.0% | |
| TGAAGATGAAGTCAATTTCT+GGG | + | chr4.4:5500929-5500948 | MS.gene030359:CDS | 30.0% | |
| TTGGTCAATTTCAGTACTTT+GGG | + | chr4.4:5501391-5501410 | MS.gene030359:CDS | 30.0% | |
| ! | GATTTTACTTGTACAAGCTA+TGG | - | chr4.4:5501074-5501093 | None:intergenic | 30.0% |
| !! | GACTATTGATTGAGTTGAAA+GGG | + | chr4.4:5501261-5501280 | MS.gene030359:intron | 30.0% |
| !! | TGACTATTGATTGAGTTGAA+AGG | + | chr4.4:5501260-5501279 | MS.gene030359:intron | 30.0% |
| !!! | AAAGGGTTTTGATTCTTATG+TGG | + | chr4.4:5501278-5501297 | MS.gene030359:intron | 30.0% |
| AAATAATATGTCTCCAACGG+AGG | + | chr4.4:5500591-5500610 | MS.gene030359:CDS | 35.0% | |
| AATAATATGTCTCCAACGGA+GGG | + | chr4.4:5500592-5500611 | MS.gene030359:CDS | 35.0% | |
| CATGAAGATGATGTAACAGT+GGG | + | chr4.4:5501330-5501349 | MS.gene030359:CDS | 35.0% | |
| CCCTGAAAATAGTTTGAGAA+GGG | + | chr4.4:5500442-5500461 | MS.gene030359:CDS | 35.0% | |
| GCATGTAAGGATTGTACATT+TGG | + | chr4.4:5501028-5501047 | MS.gene030359:intron | 35.0% | |
| GTTGGTCAATTTCAGTACTT+TGG | + | chr4.4:5501390-5501409 | MS.gene030359:CDS | 35.0% | |
| TGATGTAGATGGAAGAATTG+CGG | + | chr4.4:5501304-5501323 | MS.gene030359:intron | 35.0% | |
| ! | ACCCTTCTCAAACTATTTTC+AGG | - | chr4.4:5500446-5500465 | None:intergenic | 35.0% |
| ! | CCCTTCTCAAACTATTTTCA+GGG | - | chr4.4:5500445-5500464 | None:intergenic | 35.0% |
| ! | CCTTCTCAAACTATTTTCAG+GGG | - | chr4.4:5500444-5500463 | None:intergenic | 35.0% |
| !! | TTATGTGGCTTTGATGTAGA+TGG | + | chr4.4:5501293-5501312 | MS.gene030359:intron | 35.0% |
| AAAGTTCCTCTGTGTTCTCT+TGG | - | chr4.4:5500698-5500717 | None:intergenic | 40.0% | |
| AAGAATAGTGGTTACGGAGT+TGG | + | chr4.4:5501372-5501391 | MS.gene030359:CDS | 40.0% | |
| ATAATATGTCTCCAACGGAG+GGG | + | chr4.4:5500593-5500612 | MS.gene030359:CDS | 40.0% | |
| CCCCTGAAAATAGTTTGAGA+AGG | + | chr4.4:5500441-5500460 | MS.gene030359:CDS | 40.0% | |
| CTGATTAAACATGGAGCCTT+AGG | - | chr4.4:5500906-5500925 | None:intergenic | 40.0% | |
| GCATGAAGATGATGTAACAG+TGG | + | chr4.4:5501329-5501348 | MS.gene030359:CDS | 40.0% | |
| CAAAGTAGGTCGTTATAGCG+CGG | + | chr4.4:5500639-5500658 | MS.gene030359:CDS | 45.0% | |
| TTGAGAAGGGTTTGTAGCAC+AGG | + | chr4.4:5500455-5500474 | MS.gene030359:CDS | 45.0% | |
| ! | TTGTAGCACAGGTGATTTGC+AGG | + | chr4.4:5500466-5500485 | MS.gene030359:CDS | 45.0% |
| !! | TGTTCTCTTGGCTCTGTACT+TGG | - | chr4.4:5500686-5500705 | None:intergenic | 45.0% |
| ACAGAGCCAAGAGAACACAG+AGG | + | chr4.4:5500689-5500708 | MS.gene030359:CDS | 50.0% | |
| AACGGAGGGGAATTCGCAAG+AGG | + | chr4.4:5500606-5500625 | MS.gene030359:CDS | 55.0% | |
| GGAGCCTTAGGAATCTCCGT+GGG | - | chr4.4:5500894-5500913 | None:intergenic | 55.0% | |
| TCTTGCGAATTCCCCTCCGT+TGG | - | chr4.4:5500607-5500626 | None:intergenic | 55.0% | |
| TGGAGCCTTAGGAATCTCCG+TGG | - | chr4.4:5500895-5500914 | None:intergenic | 55.0% | |
| CGAGCCCACGGAGATTCCTA+AGG | + | chr4.4:5500887-5500906 | MS.gene030359:CDS | 60.0% | |
| GACAATCGACCACGCGTACG+TGG | + | chr4.4:5500846-5500865 | MS.gene030359:CDS | 60.0% | |
| TGCACGCAATGACGAGCCCA+CGG | + | chr4.4:5500875-5500894 | MS.gene030359:CDS | 60.0% | |
| ATCGACCACGCGTACGTGGC+AGG | + | chr4.4:5500850-5500869 | MS.gene030359:CDS | 65.0% | |
| GCAAACCTGCCACGTACGCG+TGG | - | chr4.4:5500858-5500877 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 5500329 | 5501419 | 5500329 | ID=MS.gene030359 |
| chr4.4 | mRNA | 5500329 | 5501419 | 5500329 | ID=MS.gene030359.t1;Parent=MS.gene030359 |
| chr4.4 | exon | 5500329 | 5500487 | 5500329 | ID=MS.gene030359.t1.exon1;Parent=MS.gene030359.t1 |
| chr4.4 | CDS | 5500329 | 5500487 | 5500329 | ID=cds.MS.gene030359.t1;Parent=MS.gene030359.t1 |
| chr4.4 | exon | 5500580 | 5500732 | 5500580 | ID=MS.gene030359.t1.exon2;Parent=MS.gene030359.t1 |
| chr4.4 | CDS | 5500580 | 5500732 | 5500580 | ID=cds.MS.gene030359.t1;Parent=MS.gene030359.t1 |
| chr4.4 | exon | 5500822 | 5500950 | 5500822 | ID=MS.gene030359.t1.exon3;Parent=MS.gene030359.t1 |
| chr4.4 | CDS | 5500822 | 5500950 | 5500822 | ID=cds.MS.gene030359.t1;Parent=MS.gene030359.t1 |
| chr4.4 | exon | 5501312 | 5501419 | 5501312 | ID=MS.gene030359.t1.exon4;Parent=MS.gene030359.t1 |
| chr4.4 | CDS | 5501312 | 5501419 | 5501312 | ID=cds.MS.gene030359.t1;Parent=MS.gene030359.t1 |
| Gene Sequence |
| Protein sequence |
