Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030483.t1 | XP_003630555.2 | 91.8 | 195 | 1 | 1 | 1 | 195 | 1 | 180 | 8.30E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030483.t1 | Q8S8C6 | 52.9 | 70 | 17 | 2 | 1 | 69 | 1 | 55 | 1.7e-09 | 64.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030483.t1 | G7LI34 | 91.8 | 195 | 1 | 1 | 1 | 195 | 1 | 180 | 6.0e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene030483.t1 | TF | BBR-BPC |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030483.t1 | MTR_8g099780 | 91.795 | 195 | 1 | 1 | 1 | 195 | 1 | 180 | 6.58e-125 | 355 |
| MS.gene030483.t1 | MTR_1g023660 | 41.584 | 202 | 83 | 10 | 3 | 194 | 2 | 178 | 2.58e-29 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030483.t1 | AT2G21240 | 53.788 | 132 | 43 | 3 | 1 | 131 | 1 | 115 | 4.05e-35 | 125 |
| MS.gene030483.t1 | AT2G21240 | 53.788 | 132 | 43 | 3 | 1 | 131 | 1 | 115 | 4.05e-35 | 125 |
| MS.gene030483.t1 | AT4G38910 | 39.037 | 187 | 79 | 4 | 1 | 187 | 1 | 152 | 1.57e-30 | 113 |
| MS.gene030483.t1 | AT4G38910 | 42.553 | 141 | 63 | 3 | 48 | 187 | 4 | 127 | 1.97e-22 | 91.7 |
| MS.gene030483.t1 | AT5G42520 | 35.500 | 200 | 83 | 6 | 1 | 184 | 1 | 170 | 7.13e-18 | 80.5 |
| MS.gene030483.t1 | AT5G42520 | 35.000 | 200 | 80 | 5 | 1 | 184 | 1 | 166 | 3.70e-17 | 78.6 |
Find 50 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACTATTGGGTTGATTTAT+TGG | 0.168166 | 4.2:-7379903 | MS.gene030483:CDS |
| CTTCTGTCATGTTGGATATA+TGG | 0.216505 | 4.2:+7379543 | None:intergenic |
| TCAGAAGTTATAACAGTAAC+TGG | 0.272157 | 4.2:+7379479 | None:intergenic |
| TTAACTTGCAGTTGCACTAT+TGG | 0.294219 | 4.2:-7379916 | MS.gene030483:intron |
| TTTGTTCCTTTATTTGATGC+TGG | 0.301136 | 4.2:+7379846 | None:intergenic |
| TAACTTGCAGTTGCACTATT+GGG | 0.343233 | 4.2:-7379915 | MS.gene030483:intron |
| TTGTTCCTTTATTTGATGCT+GGG | 0.361945 | 4.2:+7379847 | None:intergenic |
| ATTGGGTTGATTTATTGGAT+TGG | 0.407470 | 4.2:-7379898 | MS.gene030483:CDS |
| CGTTGAATTCCACCATTGAA+TGG | 0.413813 | 4.2:+7379596 | None:intergenic |
| CAGTACTATTCCGACTTTGA+AGG | 0.424329 | 4.2:+7379627 | None:intergenic |
| TGCCTGCCTTTCAGCCATAA+TGG | 0.435049 | 4.2:+7379793 | None:intergenic |
| GGATCCCCAGCATCAAATAA+AGG | 0.448615 | 4.2:-7379852 | MS.gene030483:CDS |
| GGATAATGCTCTACTGGAAC+GGG | 0.450868 | 4.2:-7379666 | MS.gene030483:CDS |
| ACTGGCAACTTTCCATTCAA+TGG | 0.459914 | 4.2:-7379608 | MS.gene030483:CDS |
| ATCTGAAAAGAATGAAGCCC+TGG | 0.461756 | 4.2:-7379738 | MS.gene030483:CDS |
| GTGCTTCATCCCTCTGCCGA+AGG | 0.475401 | 4.2:+7379699 | None:intergenic |
| GTCCATTATGGCTGAAAGGC+AGG | 0.476045 | 4.2:-7379795 | MS.gene030483:CDS |
| TGGATATATGGTTTGAAGAA+TGG | 0.483617 | 4.2:+7379555 | None:intergenic |
| TGAAGAATGGTGCATCCGCT+TGG | 0.493558 | 4.2:+7379568 | None:intergenic |
| TGAAAACGGTAGGCATAAGA+TGG | 0.514492 | 4.2:-7380004 | MS.gene030483:CDS |
| TCAGAGGGATAATGCTCTAC+TGG | 0.518249 | 4.2:-7379672 | MS.gene030483:CDS |
| GGGCTACGTCTCGAGCAGCC+AGG | 0.518686 | 4.2:+7379720 | None:intergenic |
| CTTCAAAGTCGGAATAGTAC+TGG | 0.519466 | 4.2:-7379626 | MS.gene030483:CDS |
| TGTTCCTTTATTTGATGCTG+GGG | 0.520374 | 4.2:+7379848 | None:intergenic |
| AGTACTATTCCGACTTTGAA+GGG | 0.520694 | 4.2:+7379628 | None:intergenic |
| GGATGAAGCACTTGCTCAGA+GGG | 0.522036 | 4.2:-7379687 | MS.gene030483:CDS |
| ATATGCTGCTTCTGTCATGT+TGG | 0.532878 | 4.2:+7379535 | None:intergenic |
| GAGACGTAGCCCTTCGGCAG+AGG | 0.546456 | 4.2:-7379709 | MS.gene030483:CDS |
| GGCTACGTCTCGAGCAGCCA+GGG | 0.552588 | 4.2:+7379721 | None:intergenic |
| CAGAAGTTATAACAGTAACT+GGG | 0.557616 | 4.2:+7379480 | None:intergenic |
| TTTAAATGTGCAGTGGAATG+TGG | 0.558628 | 4.2:-7379873 | MS.gene030483:CDS |
| CTGCTCGAGACGTAGCCCTT+CGG | 0.563490 | 4.2:-7379715 | MS.gene030483:CDS |
| CTACAGAGGAGCACATTCCC+TGG | 0.565304 | 4.2:-7379977 | MS.gene030483:intron |
| AGACGTAGCCCTTCGGCAGA+GGG | 0.565616 | 4.2:-7379708 | MS.gene030483:CDS |
| CTCTTGCAGCCCTTCAAAGT+CGG | 0.574408 | 4.2:-7379637 | MS.gene030483:CDS |
| TGCTTCATCCCTCTGCCGAA+GGG | 0.577861 | 4.2:+7379700 | None:intergenic |
| GGGATAATGCTCTACTGGAA+CGG | 0.583003 | 4.2:-7379667 | MS.gene030483:CDS |
| TCTTCTTGAACTTGAACTAG+AGG | 0.590105 | 4.2:-7379768 | MS.gene030483:CDS |
| TTAGGTCCATTATGGCTGAA+AGG | 0.590519 | 4.2:-7379799 | MS.gene030483:CDS |
| GATGACCGCCAATATGAAAA+CGG | 0.592001 | 4.2:-7380018 | MS.gene030483:CDS |
| GGCAACTTTCCATTCAATGG+TGG | 0.607937 | 4.2:-7379605 | MS.gene030483:CDS |
| CATAAGATGGACTACTACAG+AGG | 0.628336 | 4.2:-7379991 | MS.gene030483:CDS |
| TCATCTAACTAAGAGAACAA+AGG | 0.650253 | 4.2:-7379444 | MS.gene030483:CDS |
| ACCGCCAATATGAAAACGGT+AGG | 0.665966 | 4.2:-7380014 | MS.gene030483:CDS |
| GGGATGAAGCACTTGCTCAG+AGG | 0.669930 | 4.2:-7379688 | MS.gene030483:CDS |
| AGTTATAACAGTAACTGGGG+AGG | 0.670600 | 4.2:+7379484 | None:intergenic |
| AGAAGTTATAACAGTAACTG+GGG | 0.682187 | 4.2:+7379481 | None:intergenic |
| GAATTCAACGTGGATCCAAG+CGG | 0.708406 | 4.2:-7379583 | MS.gene030483:CDS |
| TTCAATGGTGGAATTCAACG+TGG | 0.712678 | 4.2:-7379593 | MS.gene030483:CDS |
| AGAAGCAGCATATAGCACGA+CGG | 0.752304 | 4.2:-7379525 | MS.gene030483:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAGTTATGAATAAAAAAATT+AGG | - | chr4.2:7379616-7379635 | MS.gene030483:CDS | 10.0% |
| !!! | AATTTTTTTATTCATAACTA+GGG | + | chr4.2:7379616-7379635 | None:intergenic | 10.0% |
| !!! | TAATTTTTTTATTCATAACT+AGG | + | chr4.2:7379617-7379636 | None:intergenic | 10.0% |
| !! | TAAAAAAATTAGGTCCATTA+TGG | - | chr4.2:7379626-7379645 | MS.gene030483:CDS | 20.0% |
| AGAAGTTATAACAGTAACTG+GGG | + | chr4.2:7379955-7379974 | None:intergenic | 30.0% | |
| CAGAAGTTATAACAGTAACT+GGG | + | chr4.2:7379956-7379975 | None:intergenic | 30.0% | |
| TCAGAAGTTATAACAGTAAC+TGG | + | chr4.2:7379957-7379976 | None:intergenic | 30.0% | |
| TCATCTAACTAAGAGAACAA+AGG | - | chr4.2:7379989-7380008 | MS.gene030483:CDS | 30.0% | |
| TGGATATATGGTTTGAAGAA+TGG | + | chr4.2:7379881-7379900 | None:intergenic | 30.0% | |
| ! | ATTGGATTTTAAATGTGCAG+TGG | - | chr4.2:7379553-7379572 | MS.gene030483:CDS | 30.0% |
| ! | TTGTTCCTTTATTTGATGCT+GGG | + | chr4.2:7379589-7379608 | None:intergenic | 30.0% |
| ! | TTTGTTCCTTTATTTGATGC+TGG | + | chr4.2:7379590-7379609 | None:intergenic | 30.0% |
| !! | ATTGGGTTGATTTATTGGAT+TGG | - | chr4.2:7379535-7379554 | MS.gene030483:CDS | 30.0% |
| AGTACTATTCCGACTTTGAA+GGG | + | chr4.2:7379808-7379827 | None:intergenic | 35.0% | |
| ATATGTGCATTCTACTTACC+AGG | + | chr4.2:7379477-7379496 | None:intergenic | 35.0% | |
| CTTCTGTCATGTTGGATATA+TGG | + | chr4.2:7379893-7379912 | None:intergenic | 35.0% | |
| TATGTGCATTCTACTTACCA+GGG | + | chr4.2:7379476-7379495 | None:intergenic | 35.0% | |
| TCTTCTTGAACTTGAACTAG+AGG | - | chr4.2:7379665-7379684 | MS.gene030483:CDS | 35.0% | |
| TTTAAATGTGCAGTGGAATG+TGG | - | chr4.2:7379560-7379579 | MS.gene030483:CDS | 35.0% | |
| ! | TAACTTGCAGTTGCACTATT+GGG | - | chr4.2:7379518-7379537 | MS.gene030483:CDS | 35.0% |
| ! | TATGCCTACCGTTTTCATAT+TGG | + | chr4.2:7379426-7379445 | None:intergenic | 35.0% |
| ! | TGTTCCTTTATTTGATGCTG+GGG | + | chr4.2:7379588-7379607 | None:intergenic | 35.0% |
| !! | GCACTATTGGGTTGATTTAT+TGG | - | chr4.2:7379530-7379549 | MS.gene030483:CDS | 35.0% |
| !! | TTAACTTGCAGTTGCACTAT+TGG | - | chr4.2:7379517-7379536 | MS.gene030483:CDS | 35.0% |
| ACTGGCAACTTTCCATTCAA+TGG | - | chr4.2:7379825-7379844 | MS.gene030483:CDS | 40.0% | |
| AGTTATAACAGTAACTGGGG+AGG | + | chr4.2:7379952-7379971 | None:intergenic | 40.0% | |
| ATCTGAAAAGAATGAAGCCC+TGG | - | chr4.2:7379695-7379714 | MS.gene030483:CDS | 40.0% | |
| CAGTACTATTCCGACTTTGA+AGG | + | chr4.2:7379809-7379828 | None:intergenic | 40.0% | |
| CGTTGAATTCCACCATTGAA+TGG | + | chr4.2:7379840-7379859 | None:intergenic | 40.0% | |
| CTTCAAAGTCGGAATAGTAC+TGG | - | chr4.2:7379807-7379826 | MS.gene030483:CDS | 40.0% | |
| GATGACCGCCAATATGAAAA+CGG | - | chr4.2:7379415-7379434 | MS.gene030483:CDS | 40.0% | |
| TGAAAACGGTAGGCATAAGA+TGG | - | chr4.2:7379429-7379448 | MS.gene030483:CDS | 40.0% | |
| TTAGGTCCATTATGGCTGAA+AGG | - | chr4.2:7379634-7379653 | MS.gene030483:CDS | 40.0% | |
| ! | ATATGCTGCTTCTGTCATGT+TGG | + | chr4.2:7379901-7379920 | None:intergenic | 40.0% |
| ! | TTCAATGGTGGAATTCAACG+TGG | - | chr4.2:7379840-7379859 | MS.gene030483:CDS | 40.0% |
| !! | CATAAGATGGACTACTACAG+AGG | - | chr4.2:7379442-7379461 | MS.gene030483:CDS | 40.0% |
| ACCGCCAATATGAAAACGGT+AGG | - | chr4.2:7379419-7379438 | MS.gene030483:CDS | 45.0% | |
| AGAAGCAGCATATAGCACGA+CGG | - | chr4.2:7379908-7379927 | MS.gene030483:CDS | 45.0% | |
| GAATTCAACGTGGATCCAAG+CGG | - | chr4.2:7379850-7379869 | MS.gene030483:CDS | 45.0% | |
| GGATAATGCTCTACTGGAAC+GGG | - | chr4.2:7379767-7379786 | MS.gene030483:CDS | 45.0% | |
| GGATCCCCAGCATCAAATAA+AGG | - | chr4.2:7379581-7379600 | MS.gene030483:CDS | 45.0% | |
| GGCAACTTTCCATTCAATGG+TGG | - | chr4.2:7379828-7379847 | MS.gene030483:CDS | 45.0% | |
| GGGATAATGCTCTACTGGAA+CGG | - | chr4.2:7379766-7379785 | MS.gene030483:CDS | 45.0% | |
| TCAGAGGGATAATGCTCTAC+TGG | - | chr4.2:7379761-7379780 | MS.gene030483:CDS | 45.0% | |
| ! | GCCTACCGTTTTCATATTGG+CGG | + | chr4.2:7379423-7379442 | None:intergenic | 45.0% |
| CTCTTGCAGCCCTTCAAAGT+CGG | - | chr4.2:7379796-7379815 | MS.gene030483:CDS | 50.0% | |
| GTCCATTATGGCTGAAAGGC+AGG | - | chr4.2:7379638-7379657 | MS.gene030483:CDS | 50.0% | |
| TGAAGAATGGTGCATCCGCT+TGG | + | chr4.2:7379868-7379887 | None:intergenic | 50.0% | |
| TGCCTGCCTTTCAGCCATAA+TGG | + | chr4.2:7379643-7379662 | None:intergenic | 50.0% | |
| ! | GGATGAAGCACTTGCTCAGA+GGG | - | chr4.2:7379746-7379765 | MS.gene030483:CDS | 50.0% |
| TGCTTCATCCCTCTGCCGAA+GGG | + | chr4.2:7379736-7379755 | None:intergenic | 55.0% | |
| ! | CTACAGAGGAGCACATTCCC+TGG | - | chr4.2:7379456-7379475 | MS.gene030483:CDS | 55.0% |
| ! | GGGATGAAGCACTTGCTCAG+AGG | - | chr4.2:7379745-7379764 | MS.gene030483:CDS | 55.0% |
| AGACGTAGCCCTTCGGCAGA+GGG | - | chr4.2:7379725-7379744 | MS.gene030483:CDS | 60.0% | |
| CTGCTCGAGACGTAGCCCTT+CGG | - | chr4.2:7379718-7379737 | MS.gene030483:CDS | 60.0% | |
| GTGCTTCATCCCTCTGCCGA+AGG | + | chr4.2:7379737-7379756 | None:intergenic | 60.0% | |
| GAGACGTAGCCCTTCGGCAG+AGG | - | chr4.2:7379724-7379743 | MS.gene030483:CDS | 65.0% | |
| GGCTACGTCTCGAGCAGCCA+GGG | + | chr4.2:7379715-7379734 | None:intergenic | 65.0% | |
| GGGCTACGTCTCGAGCAGCC+AGG | + | chr4.2:7379716-7379735 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 7379409 | 7380046 | 7379409 | ID=MS.gene030483 |
| chr4.2 | mRNA | 7379409 | 7380046 | 7379409 | ID=MS.gene030483.t1;Parent=MS.gene030483 |
| chr4.2 | exon | 7379978 | 7380046 | 7379978 | ID=MS.gene030483.t1.exon1;Parent=MS.gene030483.t1 |
| chr4.2 | CDS | 7379978 | 7380046 | 7379978 | ID=cds.MS.gene030483.t1;Parent=MS.gene030483.t1 |
| chr4.2 | exon | 7379409 | 7379927 | 7379409 | ID=MS.gene030483.t1.exon2;Parent=MS.gene030483.t1 |
| chr4.2 | CDS | 7379409 | 7379927 | 7379409 | ID=cds.MS.gene030483.t1;Parent=MS.gene030483.t1 |
| Gene Sequence |
| Protein sequence |
