AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030486

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030486.t1 MTR_8g099880 95.489 266 8 1 1 266 66 327 1.71e-166 464
MS.gene030486.t1 MTR_3g498825 60.886 271 89 7 1 266 72 330 8.46e-94 280
MS.gene030486.t1 MTR_7g053410 50.532 188 67 7 22 187 234 417 5.35e-45 158
MS.gene030486.t1 MTR_7g053410 50.532 188 67 7 22 187 234 417 8.11e-45 158
MS.gene030486.t1 MTR_5g037250 54.305 151 59 2 14 155 34 183 1.75e-43 148
MS.gene030486.t1 MTR_1g017350 50.602 166 71 4 12 171 184 344 2.74e-43 152
MS.gene030486.t1 MTR_1g017350 50.602 166 71 4 12 171 184 344 3.14e-43 152
MS.gene030486.t1 MTR_1g017350 50.602 166 71 4 12 171 184 344 3.22e-43 152
MS.gene030486.t1 MTR_1g017350 50.602 166 71 4 12 171 184 344 4.40e-43 152
MS.gene030486.t1 MTR_8g065740 61.719 128 43 2 37 164 263 384 8.49e-42 149
MS.gene030486.t1 MTR_8g012290 65.546 119 36 2 55 173 264 377 2.59e-41 148
MS.gene030486.t1 MTR_8g012290 65.546 119 36 2 55 173 264 377 2.76e-41 148
MS.gene030486.t1 MTR_3g116770 82.558 86 14 1 79 164 186 270 5.36e-41 145
MS.gene030486.t1 MTR_3g116770 82.558 86 14 1 79 164 186 270 5.54e-41 145
MS.gene030486.t1 MTR_8g062240 55.479 146 53 2 22 155 77 222 6.99e-41 142
MS.gene030486.t1 MTR_4g070320 82.143 84 14 1 81 164 140 222 5.06e-40 142
MS.gene030486.t1 MTR_5g048860 76.289 97 19 3 81 176 362 455 5.37e-40 145
MS.gene030486.t1 MTR_1g059270 69.388 98 25 3 73 170 151 243 1.10e-39 138
MS.gene030486.t1 MTR_1g059270 66.667 102 29 3 69 170 147 243 3.81e-39 139
MS.gene030486.t1 MTR_7g092510 61.667 120 37 4 81 195 170 285 1.01e-37 135
MS.gene030486.t1 MTR_6g084120 44.000 150 61 5 22 168 103 232 6.40e-26 102
MS.gene030486.t1 MTR_4g131160 57.831 83 30 2 84 162 196 277 3.24e-21 92.4
MS.gene030486.t1 MTR_4g131160 57.831 83 30 2 84 162 199 280 3.62e-21 92.0
MS.gene030486.t1 MTR_1g080890 65.152 66 22 1 84 149 193 257 2.68e-19 86.3
MS.gene030486.t1 MTR_4g033295 55.385 65 29 0 84 148 118 182 1.10e-15 73.9
MS.gene030486.t1 MTR_4g033295 53.731 67 30 1 84 149 118 184 2.25e-15 72.8
MS.gene030486.t1 MTR_4g065870 56.923 65 28 0 84 148 355 419 3.99e-15 75.1
MS.gene030486.t1 MTR_4g065870 56.452 62 27 0 84 145 355 416 2.27e-14 72.8
MS.gene030486.t1 MTR_1g069155 36.364 121 63 4 39 159 284 390 4.17e-14 72.0
MS.gene030486.t1 MTR_1g084980 38.202 89 54 1 61 149 439 526 8.71e-14 71.2
MS.gene030486.t1 MTR_7g099540 31.677 161 93 4 55 209 224 373 8.92e-14 70.9
MS.gene030486.t1 MTR_2g098310 50.746 67 33 0 82 148 330 396 9.00e-14 70.9
MS.gene030486.t1 MTR_1g070870 38.732 142 72 3 36 163 62 202 9.12e-14 70.1
MS.gene030486.t1 MTR_1g052470 50.725 69 34 0 84 152 298 366 1.05e-13 70.5
MS.gene030486.t1 MTR_7g092540 46.154 78 39 1 75 152 237 311 1.62e-13 69.7
MS.gene030486.t1 MTR_2g098310 51.515 66 32 0 82 147 330 395 2.74e-13 69.3
MS.gene030486.t1 MTR_1g070870 51.190 84 36 2 84 163 123 205 6.99e-13 67.4
MS.gene030486.t1 MTR_5g017040 38.596 114 62 4 53 159 68 180 1.01e-12 67.4
MS.gene030486.t1 MTR_7g092540 45.946 74 37 1 75 148 237 307 1.19e-12 67.0
MS.gene030486.t1 MTR_3g028520 49.231 65 33 0 84 148 322 386 4.26e-12 65.9
MS.gene030486.t1 MTR_3g027650 49.231 65 33 0 84 148 322 386 4.26e-12 65.9
MS.gene030486.t1 MTR_4g088710 55.357 56 22 2 214 266 12 67 9.66e-12 59.7
MS.gene030486.t1 MTR_1g018090 49.231 65 32 1 84 148 274 337 1.21e-11 64.3
MS.gene030486.t1 MTR_7g099540 30.539 167 93 5 55 209 224 379 1.41e-11 64.3
MS.gene030486.t1 MTR_3g027650 50.000 62 31 0 84 145 322 383 1.98e-11 63.9
MS.gene030486.t1 MTR_3g449770 33.094 139 67 5 86 212 311 435 3.35e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030486.t1 AT5G50915 65.625 128 40 1 44 171 100 223 9.07e-55 178
MS.gene030486.t1 AT5G50915 65.625 128 40 1 44 171 100 223 9.07e-55 178
MS.gene030486.t1 AT5G50915 65.625 128 40 1 44 171 100 223 1.81e-54 177
MS.gene030486.t1 AT5G50915 65.625 128 40 1 44 171 100 223 1.81e-54 177
MS.gene030486.t1 AT3G23690 46.018 226 87 8 30 244 132 333 2.10e-46 159
MS.gene030486.t1 AT1G26260 54.658 161 53 5 40 189 117 268 6.13e-46 157
MS.gene030486.t1 AT1G26260 54.658 161 53 5 40 189 146 297 1.16e-45 157
MS.gene030486.t1 AT1G68920 55.758 165 59 6 62 216 279 439 1.33e-45 159
MS.gene030486.t1 AT1G26260 54.658 161 53 5 40 189 168 319 1.47e-45 157
MS.gene030486.t1 AT1G26260 54.658 161 53 5 40 189 168 319 1.47e-45 157
MS.gene030486.t1 AT1G26260 54.658 161 53 5 40 189 168 319 1.47e-45 157
MS.gene030486.t1 AT1G26260 54.658 161 53 5 40 189 168 319 1.47e-45 157
MS.gene030486.t1 AT1G68920 55.758 165 59 6 62 216 280 440 1.50e-45 159
MS.gene030486.t1 AT1G68920 55.758 165 59 6 62 216 280 440 1.50e-45 159
MS.gene030486.t1 AT1G68920 55.758 165 59 6 62 216 280 440 1.50e-45 159
MS.gene030486.t1 AT1G10120 48.889 180 74 5 7 171 114 290 1.47e-44 154
MS.gene030486.t1 AT1G10120 48.889 180 74 5 7 171 114 290 1.47e-44 154
MS.gene030486.t1 AT3G07340 76.699 103 22 2 62 164 237 337 3.53e-44 155
MS.gene030486.t1 AT3G07340 76.699 103 22 2 62 164 237 337 3.94e-44 155
MS.gene030486.t1 AT1G59640 55.862 145 63 1 12 155 63 207 1.00e-42 147
MS.gene030486.t1 AT5G48560 80.899 89 17 0 76 164 293 381 1.03e-42 151
MS.gene030486.t1 AT5G48560 80.899 89 17 0 76 164 293 381 1.69e-42 151
MS.gene030486.t1 AT1G59640 66.957 115 38 0 41 155 93 207 2.70e-42 147
MS.gene030486.t1 AT4G34530 82.558 86 14 1 79 164 167 251 2.78e-41 145
MS.gene030486.t1 AT5G62610 53.425 146 51 3 42 170 101 246 8.29e-39 137
MS.gene030486.t1 AT5G62610 53.425 146 51 3 42 170 101 246 8.29e-39 137
MS.gene030486.t1 AT3G57800 71.875 96 22 3 75 170 197 287 1.64e-38 139
MS.gene030486.t1 AT2G42300 59.677 124 47 2 47 170 148 268 2.79e-38 135
MS.gene030486.t1 AT2G42300 59.677 124 47 2 47 170 148 268 1.42e-37 135
MS.gene030486.t1 AT2G18300 50.993 151 71 2 6 156 110 257 8.86e-37 133
MS.gene030486.t1 AT2G18300 50.993 151 71 2 6 156 110 257 9.55e-37 133
MS.gene030486.t1 AT2G18300 50.993 151 71 2 6 156 110 257 1.71e-36 133
MS.gene030486.t1 AT4G36540 78.947 76 16 0 81 156 138 213 2.77e-36 131
MS.gene030486.t1 AT4G36540 57.851 121 40 3 43 156 97 213 2.86e-36 131
MS.gene030486.t1 AT1G18400 67.816 87 27 1 82 168 143 228 5.64e-35 126
MS.gene030486.t1 AT1G18400 67.816 87 27 1 82 168 143 228 7.24e-35 126
MS.gene030486.t1 AT1G73830 67.442 86 27 1 83 168 146 230 7.39e-34 124
MS.gene030486.t1 AT1G73830 67.442 86 27 1 83 168 146 230 8.65e-34 123
MS.gene030486.t1 AT1G25330 52.991 117 37 2 56 172 89 187 1.71e-33 121
MS.gene030486.t1 AT3G57800 48.951 143 21 4 75 170 197 334 4.94e-30 117
MS.gene030486.t1 AT5G58010 50.962 104 38 3 84 183 99 193 3.16e-21 90.9
MS.gene030486.t1 AT2G24260 62.500 72 26 1 78 149 29 99 3.43e-21 89.7
MS.gene030486.t1 AT4G30980 68.182 66 20 1 84 149 130 194 1.29e-20 89.4
MS.gene030486.t1 AT4G30980 68.182 66 20 1 84 149 178 242 1.56e-20 90.1
MS.gene030486.t1 AT2G24260 59.211 76 30 1 74 149 128 202 2.53e-20 89.4
MS.gene030486.t1 AT2G42300 58.442 77 32 0 47 123 148 224 4.31e-19 84.0
MS.gene030486.t1 AT2G42300 58.442 77 32 0 47 123 148 224 4.31e-19 84.0
MS.gene030486.t1 AT2G42300 58.442 77 32 0 47 123 148 224 5.49e-19 83.6
MS.gene030486.t1 AT4G28800 45.833 72 38 1 87 158 252 322 9.19e-14 70.9
MS.gene030486.t1 AT4G28815 38.318 107 56 4 87 190 71 170 1.11e-13 68.9
MS.gene030486.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.12e-13 70.9
MS.gene030486.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.12e-13 70.9
MS.gene030486.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.12e-13 70.9
MS.gene030486.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.12e-13 70.9
MS.gene030486.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.12e-13 70.9
MS.gene030486.t1 AT1G09530 41.573 89 48 2 61 149 317 401 1.12e-13 70.9
MS.gene030486.t1 AT1G35460 50.769 65 32 0 84 148 181 245 1.75e-13 68.9
MS.gene030486.t1 AT1G51140 54.688 64 29 0 84 147 304 367 2.86e-13 69.3
MS.gene030486.t1 AT4G28815 38.318 107 56 4 87 190 147 246 4.16e-13 68.6
MS.gene030486.t1 AT2G20180 49.206 63 31 1 87 149 210 271 4.65e-13 68.9
MS.gene030486.t1 AT2G20180 49.206 63 31 1 87 149 210 271 4.65e-13 68.9
MS.gene030486.t1 AT2G20180 49.206 63 31 1 87 149 210 271 4.65e-13 68.9
MS.gene030486.t1 AT2G42280 50.000 64 32 0 84 147 279 342 4.98e-13 68.6
MS.gene030486.t1 AT2G20180 49.206 63 31 1 87 149 281 342 5.04e-13 68.9
MS.gene030486.t1 AT2G20180 49.206 63 31 1 87 149 281 342 5.04e-13 68.9
MS.gene030486.t1 AT2G20180 49.206 63 31 1 87 149 281 342 5.04e-13 68.9
MS.gene030486.t1 AT2G20180 49.206 63 31 1 87 149 281 342 5.04e-13 68.9
MS.gene030486.t1 AT4G09180 49.254 67 34 0 82 148 182 248 5.07e-13 67.8
MS.gene030486.t1 AT4G28800 42.169 83 44 2 87 166 264 345 5.53e-13 68.6
MS.gene030486.t1 AT2G42280 51.515 66 30 1 84 147 279 344 9.16e-13 67.8
MS.gene030486.t1 AT4G28790 36.735 98 58 2 62 159 252 345 1.14e-12 67.8
MS.gene030486.t1 AT4G02590 50.000 84 37 2 84 163 83 165 1.68e-12 65.9
MS.gene030486.t1 AT4G02590 50.000 84 37 2 84 163 146 228 2.85e-12 65.9
MS.gene030486.t1 AT4G02590 50.000 84 37 2 84 163 146 228 2.85e-12 65.9
MS.gene030486.t1 AT1G03040 59.091 66 26 1 84 149 139 203 9.86e-12 64.3
MS.gene030486.t1 AT1G03040 59.091 66 26 1 84 149 144 208 1.04e-11 64.3
MS.gene030486.t1 AT4G33880 45.783 83 42 3 84 165 266 346 1.36e-11 64.3
MS.gene030486.t1 AT1G03040 59.091 66 26 1 84 149 144 208 1.62e-11 63.9
MS.gene030486.t1 AT2G20180 49.153 59 29 1 87 145 281 338 1.87e-11 63.9
MS.gene030486.t1 AT2G43140 43.590 78 42 1 72 147 120 197 2.44e-11 62.0
MS.gene030486.t1 AT2G43140 43.590 78 42 1 72 147 217 294 3.29e-11 62.8
MS.gene030486.t1 AT4G00050 44.595 74 40 1 75 148 96 168 4.69e-11 62.4

Find 62 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAAATTGATATTAATTTA+AGG 0.098761 4.2:-7462028 None:intergenic
AGCACACTGATGGACCATTT+TGG 0.191470 4.2:+7461944 MS.gene030486:intron
TGTTAATAATGACTATGTTT+TGG 0.279340 4.2:+7461265 MS.gene030486:CDS
TCAGAAAGCTGTTACTTCTA+TGG 0.295160 4.2:+7459896 MS.gene030486:CDS
TTTCATATTAAATAGGTAAC+TGG 0.313052 4.2:+7460780 MS.gene030486:intron
TTTGAGTTGCCCCTTGTCTA+AGG 0.332895 4.2:+7459950 MS.gene030486:CDS
CAAAAGCTTCTTAATCCATA+TGG 0.364693 4.2:+7461985 MS.gene030486:CDS
ATTGCAAAGTCAAAGAAGAT+TGG 0.375639 4.2:-7460988 None:intergenic
TATTTCCTCACCTGATGATC+TGG 0.386280 4.2:-7461036 None:intergenic
AAAACATAGTCATTATTAAC+AGG 0.386747 4.2:-7461263 None:intergenic
CTTCTTAATCCATATGGATT+CGG 0.417843 4.2:+7461991 MS.gene030486:CDS
AACTTGTGCCTGGCTGTGAT+AGG 0.422109 4.2:+7460542 MS.gene030486:CDS
TAGCCACAGCCTTGCTGAAA+GGG 0.444848 4.2:+7460233 MS.gene030486:CDS
ACATGTGGTATTGGTGTTGA+TGG 0.451696 4.2:-7461179 None:intergenic
CACAATGGTACATGTGGTAT+TGG 0.457363 4.2:-7461188 None:intergenic
TGCTCTTCTACGTCCCAAAA+TGG 0.463393 4.2:-7461958 None:intergenic
AGCAGTGGTGAAGGGATGAA+AGG 0.466227 4.2:+7460108 MS.gene030486:CDS
TTGTAGGATGTCTCAAAAGA+AGG 0.474006 4.2:+7460063 MS.gene030486:intron
GTGTTGTTGCCGAATCCATA+TGG 0.475225 4.2:-7462000 None:intergenic
GGTAGTGGACAATCTTGAAA+AGG 0.476116 4.2:+7459860 MS.gene030486:CDS
AATATTGAAGAAGTTCCAAC+TGG 0.478486 4.2:+7460171 MS.gene030486:CDS
GACCCCTTCTTGCTCTGACA+TGG 0.481977 4.2:-7460200 None:intergenic
CTTGGTTAGACCAGATCATC+AGG 0.485638 4.2:+7461026 MS.gene030486:CDS
AATCTTCTTTGACTTTGCAA+TGG 0.497293 4.2:+7460990 MS.gene030486:CDS
ATCAAATTTACCTTAGACAA+GGG 0.503038 4.2:-7459960 None:intergenic
CAAAAGAAGAGCAGTGGTGA+AGG 0.507125 4.2:+7460099 MS.gene030486:CDS
AGCAAAAGGTGTGGCATGGT+TGG 0.509890 4.2:-7461216 None:intergenic
TACTGAATCATCCATGGTAG+TGG 0.513020 4.2:+7459845 None:intergenic
TGTCAGAGCAAGAAGGGGTC+AGG 0.517190 4.2:+7460203 MS.gene030486:CDS
ATATTGCAGCAACTTGTGCC+TGG 0.524669 4.2:+7460532 MS.gene030486:CDS
GTGGTAATTGTGTCAGCAAA+AGG 0.534827 4.2:-7461230 None:intergenic
ATAGCCACAGCCTTGCTGAA+AGG 0.535109 4.2:+7460232 MS.gene030486:CDS
ATACCCTTTCAGCAAGGCTG+TGG 0.536175 4.2:-7460236 None:intergenic
GAGGTCAAATACCTTCTTTG+AGG 0.536244 4.2:+7461319 MS.gene030486:CDS
GATCTAAGGTACCTCAAAGA+AGG 0.549335 4.2:-7461330 None:intergenic
TGTAGGATGTCTCAAAAGAA+GGG 0.553029 4.2:+7460064 MS.gene030486:intron
TATCCATGTCAGAGCAAGAA+GGG 0.554936 4.2:+7460197 MS.gene030486:CDS
GTAGTGGACAATCTTGAAAA+GGG 0.561287 4.2:+7459861 MS.gene030486:CDS
CATAGTCATTATTAACAGGA+TGG 0.563260 4.2:-7461259 None:intergenic
TTCAAGATTGTCCACTACCA+TGG 0.563562 4.2:-7459856 None:intergenic
ACTTGTGCCTGGCTGTGATA+GGG 0.572729 4.2:+7460543 MS.gene030486:CDS
AACTGGAAAGGCCCTTGTGT+TGG 0.580713 4.2:+7460797 MS.gene030486:CDS
TGGTGAAGGGATGAAAGGAG+AGG 0.583503 4.2:+7460113 MS.gene030486:CDS
CATCAAATTTACCTTAGACA+AGG 0.584025 4.2:-7459961 None:intergenic
TGATTATTTCATCCAACACA+AGG 0.586530 4.2:-7460809 None:intergenic
AATGGACCTAGACACACTCT+TGG 0.596392 4.2:+7461008 MS.gene030486:CDS
GTCCTTACAAAATCAAGTAG+AGG 0.597808 4.2:+7460842 MS.gene030486:CDS
GTCTAACCAAGAGTGTGTCT+AGG 0.610495 4.2:-7461014 None:intergenic
ATATCCATGTCAGAGCAAGA+AGG 0.615381 4.2:+7460196 MS.gene030486:CDS
AAAAGAAGAGCAGTGGTGAA+GGG 0.621109 4.2:+7460100 MS.gene030486:CDS
TATTAAATAGGTAACTGGAA+AGG 0.623233 4.2:+7460785 MS.gene030486:intron
AGACTCACCCTATCACAGCC+AGG 0.626341 4.2:-7460550 None:intergenic
GATTATTTCATCCAACACAA+GGG 0.628005 4.2:-7460808 None:intergenic
TCAAATTTACCTTAGACAAG+GGG 0.638877 4.2:-7459959 None:intergenic
TATTGCATACCCTTTCAGCA+AGG 0.648025 4.2:-7460242 None:intergenic
CTGACATGGATATAACCAGT+TGG 0.650898 4.2:-7460186 None:intergenic
ATTAACAGGATGGAAGCTTG+TGG 0.660676 4.2:-7461249 None:intergenic
ATCCATGTCAGAGCAAGAAG+GGG 0.665171 4.2:+7460198 MS.gene030486:CDS
GAGCTGCACAATGGTACATG+TGG 0.691981 4.2:-7461194 None:intergenic
TGTCAGCAAAAGGTGTGGCA+TGG 0.694030 4.2:-7461220 None:intergenic
GCATGGTTGGAGCTGCACAA+TGG 0.724748 4.2:-7461203 None:intergenic
AATTGTGTCAGCAAAAGGTG+TGG 0.745401 4.2:-7461225 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATGTTGCAAAAAAAT+TGG + chr4.2:7460469-7460488 MS.gene030486:intron 15.0%
!! ATAGAAACATATTCAAAAAA+GGG - chr4.2:7460006-7460025 None:intergenic 15.0%
!! TAATTGTTGAAGTTATTAAA+GGG - chr4.2:7461487-7461506 None:intergenic 15.0%
!! TATAGAAACATATTCAAAAA+AGG - chr4.2:7460007-7460026 None:intergenic 15.0%
!! TGATACTATATGATTATAAT+TGG + chr4.2:7461362-7461381 MS.gene030486:intron 15.0%
!! TTAATTGTTGAAGTTATTAA+AGG - chr4.2:7461488-7461507 None:intergenic 15.0%
!! TTCAATGAAAATTATTACAA+GGG - chr4.2:7460626-7460645 None:intergenic 15.0%
!!! AAGTACAACTTTTATATTTT+TGG - chr4.2:7460876-7460895 None:intergenic 15.0%
!! AAAACATAGTCATTATTAAC+AGG - chr4.2:7461266-7461285 None:intergenic 20.0%
!! CATTCCTTTAATATAGATAT+CGG + chr4.2:7460574-7460593 MS.gene030486:intron 20.0%
!! CTTCAATGAAAATTATTACA+AGG - chr4.2:7460627-7460646 None:intergenic 20.0%
!! TAGGTTATTACAATTTACAA+CGG + chr4.2:7460403-7460422 MS.gene030486:intron 20.0%
!! TATCAAAATCTTATGATCTA+AGG - chr4.2:7461347-7461366 None:intergenic 20.0%
!! TTCTAAATATAAGTCCAATA+CGG + chr4.2:7461456-7461475 MS.gene030486:intron 20.0%
!! TTTCATATTAAATAGGTAAC+TGG + chr4.2:7460780-7460799 MS.gene030486:intron 20.0%
!!! ATTAACATTTTGCAATTTCA+AGG - chr4.2:7461597-7461616 None:intergenic 20.0%
!!! GATTTGGTTTCATATTAAAT+AGG + chr4.2:7460773-7460792 MS.gene030486:intron 20.0%
!!! TAACATTTTGGAATTTTAGA+GGG + chr4.2:7461776-7461795 MS.gene030486:intron 20.0%
!!! TGTTAATAATGACTATGTTT+TGG + chr4.2:7461265-7461284 MS.gene030486:CDS 20.0%
!!! TTAACATTTTGGAATTTTAG+AGG + chr4.2:7461775-7461794 MS.gene030486:intron 20.0%
!!! TTATTGATTTTGTTACTTTG+TGG + chr4.2:7460037-7460056 MS.gene030486:intron 20.0%
!!! TTGAAATATCGGTTTTAAAT+AGG + chr4.2:7460720-7460739 MS.gene030486:intron 20.0%
!!! TTTTAAAATTACTACACTGA+TGG - chr4.2:7460327-7460346 None:intergenic 20.0%
!!! TTTTGGTAAAATGACTAAAT+TGG - chr4.2:7461867-7461886 None:intergenic 20.0%
! AAACCGATATTTCAAATTGA+AGG - chr4.2:7460715-7460734 None:intergenic 25.0%
! AAGATGAATCTTGATATTCA+AGG - chr4.2:7461636-7461655 None:intergenic 25.0%
! ATATGAAACCAAATCAAATG+TGG - chr4.2:7460768-7460787 None:intergenic 25.0%
! ATCAAATTTACCTTAGACAA+GGG - chr4.2:7459963-7459982 None:intergenic 25.0%
! ATGTCCGATATCTATATTAA+AGG - chr4.2:7460581-7460600 None:intergenic 25.0%
! ATTACAATTTACAACGGAAA+GGG + chr4.2:7460409-7460428 MS.gene030486:intron 25.0%
! ATTATTGCAAAATGTTGATC+AGG - chr4.2:7461829-7461848 None:intergenic 25.0%
! GTTTATAAAGTTAAGCTTGT+TGG - chr4.2:7460919-7460938 None:intergenic 25.0%
! TATTAAATAGGTAACTGGAA+AGG + chr4.2:7460785-7460804 MS.gene030486:intron 25.0%
! TATTACAATTTACAACGGAA+AGG + chr4.2:7460408-7460427 MS.gene030486:intron 25.0%
! TGTATGTATCAATGTTGTAT+CGG + chr4.2:7460654-7460673 MS.gene030486:intron 25.0%
! TTGCAAAAAAATTGGATTTC+AGG + chr4.2:7460477-7460496 MS.gene030486:intron 25.0%
!! CAACTTCAATTTTTCTTCAA+GGG + chr4.2:7461294-7461313 MS.gene030486:CDS 25.0%
!! GACAAATCTGATTAACATTT+TGG + chr4.2:7461764-7461783 MS.gene030486:intron 25.0%
!!! AAGAGATCGATTTTGATATT+TGG - chr4.2:7461529-7461548 None:intergenic 25.0%
!!! GATCAAAGTACTTTATGTTT+TGG - chr4.2:7461884-7461903 None:intergenic 25.0%
!!! TGTTGAGTTGATATTTTTAG+TGG - chr4.2:7461717-7461736 None:intergenic 25.0%
AAAAAACAAAAGAAGAGCAG+TGG + chr4.2:7460093-7460112 MS.gene030486:CDS 30.0%
AATATTGAAGAAGTTCCAAC+TGG + chr4.2:7460171-7460190 MS.gene030486:CDS 30.0%
ACAATGAGTTATGAACTTTG+AGG - chr4.2:7460448-7460467 None:intergenic 30.0%
ATCAACAAGTCTCTACATTA+TGG + chr4.2:7461556-7461575 MS.gene030486:intron 30.0%
ATGAATCTTGATATTCAAGG+AGG - chr4.2:7461633-7461652 None:intergenic 30.0%
ATTGCAAAGTCAAAGAAGAT+TGG - chr4.2:7460991-7461010 None:intergenic 30.0%
CAAAAGCTTCTTAATCCATA+TGG + chr4.2:7461985-7462004 MS.gene030486:CDS 30.0%
CATAGTCATTATTAACAGGA+TGG - chr4.2:7461262-7461281 None:intergenic 30.0%
CATCAAATTTACCTTAGACA+AGG - chr4.2:7459964-7459983 None:intergenic 30.0%
CCTTGAAGAAAAATTGAAGT+TGG - chr4.2:7461296-7461315 None:intergenic 30.0%
CTTCTTAATCCATATGGATT+CGG + chr4.2:7461991-7462010 MS.gene030486:CDS 30.0%
GAAGAAAAAAGAAGAGAAGA+AGG + chr4.2:7460137-7460156 MS.gene030486:CDS 30.0%
GATTATTTCATCCAACACAA+GGG - chr4.2:7460811-7460830 None:intergenic 30.0%
TCAAATTTACCTTAGACAAG+GGG - chr4.2:7459962-7459981 None:intergenic 30.0%
TGATTATTTCATCCAACACA+AGG - chr4.2:7460812-7460831 None:intergenic 30.0%
! AATCTTCTTTGACTTTGCAA+TGG + chr4.2:7460990-7461009 MS.gene030486:CDS 30.0%
! ACGCCTTCAATTTGAAATAT+CGG + chr4.2:7460709-7460728 MS.gene030486:intron 30.0%
! CCAACTTCAATTTTTCTTCA+AGG + chr4.2:7461293-7461312 MS.gene030486:CDS 30.0%
! TACCTCTACTTGATTTTGTA+AGG - chr4.2:7460847-7460866 None:intergenic 30.0%
! TATGAACTTTGAGGAAATTG+AGG - chr4.2:7460439-7460458 None:intergenic 30.0%
!! AATGTAGAGACTTGTTGATA+AGG - chr4.2:7461555-7461574 None:intergenic 30.0%
!! CGCTTGAATTTGTTATCAAA+TGG + chr4.2:7461658-7461677 MS.gene030486:intron 30.0%
!! TGTTACTTTGTGGTATTTGT+AGG + chr4.2:7460047-7460066 MS.gene030486:intron 30.0%
!!! GCTAGCTTTTCATCATAATA+AGG - chr4.2:7461911-7461930 None:intergenic 30.0%
!!! GGTTTTAAATAGGTTTACGT+AGG + chr4.2:7460730-7460749 MS.gene030486:intron 30.0%
ATTTGTCCTTGCGTTATTAG+AGG - chr4.2:7461751-7461770 None:intergenic 35.0%
CACTAACACCTATCTAGTAT+CGG + chr4.2:7460684-7460703 MS.gene030486:intron 35.0%
GTCCTTACAAAATCAAGTAG+AGG + chr4.2:7460842-7460861 MS.gene030486:CDS 35.0%
TGTAGGATGTCTCAAAAGAA+GGG + chr4.2:7460064-7460083 MS.gene030486:intron 35.0%
TTGTAGGATGTCTCAAAAGA+AGG + chr4.2:7460063-7460082 MS.gene030486:intron 35.0%
TTTCACAGCCACATTTGATT+TGG + chr4.2:7460757-7460776 MS.gene030486:intron 35.0%
TTTGTCCTTGCGTTATTAGA+GGG - chr4.2:7461750-7461769 None:intergenic 35.0%
! CTCTGTTACTGAATTTTCAG+AGG + chr4.2:7461138-7461157 MS.gene030486:intron 35.0%
! TATCATTTTCAGCACACTGA+TGG + chr4.2:7461934-7461953 MS.gene030486:intron 35.0%
! TCAGAAAGCTGTTACTTCTA+TGG + chr4.2:7459896-7459915 MS.gene030486:CDS 35.0%
!! GTAGTGGACAATCTTGAAAA+GGG + chr4.2:7459861-7459880 MS.gene030486:CDS 35.0%
AAAAGAAGAGCAGTGGTGAA+GGG + chr4.2:7460100-7460119 MS.gene030486:CDS 40.0%
AATTGTGTCAGCAAAAGGTG+TGG - chr4.2:7461228-7461247 None:intergenic 40.0%
ATATCCATGTCAGAGCAAGA+AGG + chr4.2:7460196-7460215 MS.gene030486:CDS 40.0%
ATTAACAGGATGGAAGCTTG+TGG - chr4.2:7461252-7461271 None:intergenic 40.0%
CACAATGGTACATGTGGTAT+TGG - chr4.2:7461191-7461210 None:intergenic 40.0%
CTGACATGGATATAACCAGT+TGG - chr4.2:7460189-7460208 None:intergenic 40.0%
GAGGTCAAATACCTTCTTTG+AGG + chr4.2:7461319-7461338 MS.gene030486:CDS 40.0%
GTGGTAATTGTGTCAGCAAA+AGG - chr4.2:7461233-7461252 None:intergenic 40.0%
TATCCATGTCAGAGCAAGAA+GGG + chr4.2:7460197-7460216 MS.gene030486:CDS 40.0%
TATTGCATACCCTTTCAGCA+AGG - chr4.2:7460245-7460264 None:intergenic 40.0%
TATTTCCTCACCTGATGATC+TGG - chr4.2:7461039-7461058 None:intergenic 40.0%
! AATTGGCTCATGAGATGTCA+GGG + chr4.2:7461379-7461398 MS.gene030486:intron 40.0%
! ATCAATGTTGTATCGGTGTC+AGG + chr4.2:7460661-7460680 MS.gene030486:intron 40.0%
! CAATTTTTCTTCAAGGGCAG+AGG + chr4.2:7461300-7461319 MS.gene030486:CDS 40.0%
! GATCTAAGGTACCTCAAAGA+AGG - chr4.2:7461333-7461352 None:intergenic 40.0%
! GTGTTGTCAGACAAAAGTGT+CGG + chr4.2:7460373-7460392 MS.gene030486:intron 40.0%
! TAATTGGCTCATGAGATGTC+AGG + chr4.2:7461378-7461397 MS.gene030486:intron 40.0%
! TACTACACTGATGGAAGTGT+CGG - chr4.2:7460318-7460337 None:intergenic 40.0%
!! ACATGTGGTATTGGTGTTGA+TGG - chr4.2:7461182-7461201 None:intergenic 40.0%
!! ATTAAAGGGAAGCACCGTAT+TGG - chr4.2:7461473-7461492 None:intergenic 40.0%
!! GGTAGTGGACAATCTTGAAA+AGG + chr4.2:7459860-7459879 MS.gene030486:CDS 40.0%
AATGGACCTAGACACACTCT+TGG + chr4.2:7461008-7461027 MS.gene030486:CDS 45.0%
ACAGTCCCTCTAATAACGCA+AGG + chr4.2:7461742-7461761 MS.gene030486:intron 45.0%
ATATTGCAGCAACTTGTGCC+TGG + chr4.2:7460532-7460551 MS.gene030486:CDS 45.0%
ATCCATGTCAGAGCAAGAAG+GGG + chr4.2:7460198-7460217 MS.gene030486:CDS 45.0%
CAAAAGAAGAGCAGTGGTGA+AGG + chr4.2:7460099-7460118 MS.gene030486:CDS 45.0%
CGGTGTTTGTGTTTGCGTTA+GGG - chr4.2:7460286-7460305 None:intergenic 45.0%
CTTGGTTAGACCAGATCATC+AGG + chr4.2:7461026-7461045 MS.gene030486:CDS 45.0%
TGCTCTTCTACGTCCCAAAA+TGG - chr4.2:7461961-7461980 None:intergenic 45.0%
TGTTTGCGTTAGGGCTTCAT+AGG - chr4.2:7460277-7460296 None:intergenic 45.0%
TTAGACCAGATCATCAGGTG+AGG + chr4.2:7461031-7461050 MS.gene030486:intron 45.0%
! ATTGGCTCATGAGATGTCAG+GGG + chr4.2:7461380-7461399 MS.gene030486:intron 45.0%
! GTCTAACCAAGAGTGTGTCT+AGG - chr4.2:7461017-7461036 None:intergenic 45.0%
! GTGTTGTTGCCGAATCCATA+TGG - chr4.2:7462003-7462022 None:intergenic 45.0%
! TCGGTGTTTGTGTTTGCGTT+AGG - chr4.2:7460287-7460306 None:intergenic 45.0%
!! AGCACACTGATGGACCATTT+TGG + chr4.2:7461944-7461963 MS.gene030486:intron 45.0%
!! CAAAAGTGTCGGAGCTACAT+AGG + chr4.2:7460384-7460403 MS.gene030486:intron 45.0%
!! GCACACTGATGGACCATTTT+GGG + chr4.2:7461945-7461964 MS.gene030486:intron 45.0%
!! TTTGAGTTGCCCCTTGTCTA+AGG + chr4.2:7459950-7459969 MS.gene030486:CDS 45.0%
AACTGGAAAGGCCCTTGTGT+TGG + chr4.2:7460797-7460816 MS.gene030486:CDS 50.0%
AACTTGTGCCTGGCTGTGAT+AGG + chr4.2:7460542-7460561 MS.gene030486:CDS 50.0%
ACTTGTGCCTGGCTGTGATA+GGG + chr4.2:7460543-7460562 MS.gene030486:CDS 50.0%
AGCAGTGGTGAAGGGATGAA+AGG + chr4.2:7460108-7460127 MS.gene030486:CDS 50.0%
AGGCGTGACCGATACTAGAT+AGG - chr4.2:7460695-7460714 None:intergenic 50.0%
ATACCCTTTCAGCAAGGCTG+TGG - chr4.2:7460239-7460258 None:intergenic 50.0%
ATAGCCACAGCCTTGCTGAA+AGG + chr4.2:7460232-7460251 MS.gene030486:CDS 50.0%
GAGCTGCACAATGGTACATG+TGG - chr4.2:7461197-7461216 None:intergenic 50.0%
GGACTATAACGCTGCTGAGT+TGG + chr4.2:7461797-7461816 MS.gene030486:intron 50.0%
TAGCCACAGCCTTGCTGAAA+GGG + chr4.2:7460233-7460252 MS.gene030486:CDS 50.0%
TGGTGAAGGGATGAAAGGAG+AGG + chr4.2:7460113-7460132 MS.gene030486:CDS 50.0%
! AGCAAAAGGTGTGGCATGGT+TGG - chr4.2:7461219-7461238 None:intergenic 50.0%
! TGTCAGCAAAAGGTGTGGCA+TGG - chr4.2:7461223-7461242 None:intergenic 50.0%
!! GGAAGTGTCGGTGTTGTGTT+CGG - chr4.2:7460306-7460325 None:intergenic 50.0%
AGACTCACCCTATCACAGCC+AGG - chr4.2:7460553-7460572 None:intergenic 55.0%
GACCCCTTCTTGCTCTGACA+TGG - chr4.2:7460203-7460222 None:intergenic 55.0%
GCATGGTTGGAGCTGCACAA+TGG - chr4.2:7461206-7461225 None:intergenic 55.0%
TGTCAGAGCAAGAAGGGGTC+AGG + chr4.2:7460203-7460222 MS.gene030486:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 7459858 7462038 7459858 ID=MS.gene030486
chr4.2 mRNA 7459858 7462038 7459858 ID=MS.gene030486.t1;Parent=MS.gene030486
chr4.2 exon 7459858 7459971 7459858 ID=MS.gene030486.t1.exon1;Parent=MS.gene030486.t1
chr4.2 CDS 7459858 7459971 7459858 ID=cds.MS.gene030486.t1;Parent=MS.gene030486.t1
chr4.2 exon 7460069 7460254 7460069 ID=MS.gene030486.t1.exon2;Parent=MS.gene030486.t1
chr4.2 CDS 7460069 7460254 7460069 ID=cds.MS.gene030486.t1;Parent=MS.gene030486.t1
chr4.2 exon 7460499 7460564 7460499 ID=MS.gene030486.t1.exon3;Parent=MS.gene030486.t1
chr4.2 CDS 7460499 7460564 7460499 ID=cds.MS.gene030486.t1;Parent=MS.gene030486.t1
chr4.2 exon 7460795 7460863 7460795 ID=MS.gene030486.t1.exon4;Parent=MS.gene030486.t1
chr4.2 CDS 7460795 7460863 7460795 ID=cds.MS.gene030486.t1;Parent=MS.gene030486.t1
chr4.2 exon 7460958 7461047 7460958 ID=MS.gene030486.t1.exon5;Parent=MS.gene030486.t1
chr4.2 CDS 7460958 7461047 7460958 ID=cds.MS.gene030486.t1;Parent=MS.gene030486.t1
chr4.2 exon 7461158 7461340 7461158 ID=MS.gene030486.t1.exon6;Parent=MS.gene030486.t1
chr4.2 CDS 7461158 7461340 7461158 ID=cds.MS.gene030486.t1;Parent=MS.gene030486.t1
chr4.2 exon 7461946 7462038 7461946 ID=MS.gene030486.t1.exon7;Parent=MS.gene030486.t1
chr4.2 CDS 7461946 7462038 7461946 ID=cds.MS.gene030486.t1;Parent=MS.gene030486.t1
Gene Sequence

>MS.gene030486

ATGGTAGTGGACAATCTTGAAAAGGGTGAACAAGTTACTCAGAAAGCTGTTACTTCTATGGAGAAGAAAAGAAGAATCAGAAATAAGACTTCTTTGAGTTGCCCCTTGTCTAAGGTAAATTTGATGCAGTTTTTTTTTTTTTTTACCCTTTTTTGAATATGTTTCTATATTGTTTCATGTTATTGATTTTGTTACTTTGTGGTATTTGTAGGATGTCTCAAAAGAAGGGAGAAGCAAAAAACAAAAGAAGAGCAGTGGTGAAGGGATGAAAGGAGAGGAGAAGAAAAAAGAAGAGAAGAAGGATCAAAAGAAAAATATTGAAGAAGTTCCAACTGGTTATATCCATGTCAGAGCAAGAAGGGGTCAGGCAACAGATAGCCACAGCCTTGCTGAAAGGGTATGCAATACAGTTTTTGCCTATGAAGCCCTAACGCAAACACAAACACCGAACACAACACCGACACTTCCATCAGTGTAGTAATTTTAAAAAATTGAATGTAATAACATGTGTCAGTGTGTTGTCAGACAAAAGTGTCGGAGCTACATAGGTTATTACAATTTACAACGGAAAGGGAATTCCTCAATTTCCTCAAAGTTCATAACTCATTGTTAATAAATGTTGCAAAAAAATTGGATTTCAGGTGAGAAGAGAAAAAATAAGTGAAAGAATGAAGATATTGCAGCAACTTGTGCCTGGCTGTGATAGGGTGAGTCTTCATTCCTTTAATATAGATATCGGACATAACATTGATACTGACACATTGACCCTTGTAATAATTTTCATTGAAGAAGTTATTGTATGTATCAATGTTGTATCGGTGTCAGGCACTAACACCTATCTAGTATCGGTCACGCCTTCAATTTGAAATATCGGTTTTAAATAGGTTTACGTAGGCTCTTTTCACAGCCACATTTGATTTGGTTTCATATTAAATAGGTAACTGGAAAGGCCCTTGTGTTGGATGAAATAATCAATTATGTACAGTCCTTACAAAATCAAGTAGAGGTAATGCTGCCAAAAATATAAAAGTTGTACTTTTGAATCATTGATTCATAAACCAACAAGCTTAACTTTATAAACTTCTATATTTCTGATGCAGTTCTTGTCAATGAAGCTTGCTTCAGTGAATCCAATCTTCTTTGACTTTGCAATGGACCTAGACACACTCTTGGTTAGACCAGATCATCAGGTGAGGAAATATAACTCAGTAATTAATTTTCATGTACTTGAATTTATATCAGCTGACTCAAACAAATTTATTAATGATGAAATAATGAAACTCTGTTACTGAATTTTCAGAGGTTAAATAGCATAACATCACCATCAACACCAATACCACATGTACCATTGTGCAGCTCCAACCATGCCACACCTTTTGCTGACACAATTACCACAAGCTTCCATCCTGTTAATAATGACTATGTTTTGGATTATCCAACTTCAATTTTTCTTCAAGGGCAGAGGTCAAATACCTTCTTTGAGGTACCTTAGATCATAAGATTTTGATACTATATGATTATAATTGGCTCATGAGATGTCAGGGGATACAATTATGTAATTAGCATACTAGTAAGTTTGAAAATTTTTGTCATTATTTTTCTAAATATAAGTCCAATACGGTGCTTCCCTTTAATAACTTCAACAATTAAATATATGTATACAAGTGACCAAATATCAAAATCGATCTCTTGCACCTTATCAACAAGTCTCTACATTATGGATATTTTGAAATGATCCTTGAAATTGCAAAATGTTAATCAAGTCTATAACACCTCCTTGAATATCAAGATTCATCTTTACGCTTGAATTTGTTATCAAATGGATCTCTGACATTATTCACTTAGTTCAAAATGAACCACTAAAAATATCAACTCAACATCGTTACAGTCCCTCTAATAACGCAAGGACAAATCTGATTAACATTTTGGAATTTTAGAGGGACTATAACGCTGCTGAGTTGGTACAAACCTGATCAACATTTTGCAATAATTATTAAGTAAAATATCCAATTTAGTCATTTTACCAAAACATAAAGTACTTTGATCATTTCCTTATTATGATGAAAAGCTAGCATATATCATTTTCAGCACACTGATGGACCATTTTGGGACGTAGAAGAGCAACGACAAAAGCTTCTTAATCCATATGGATTCGGCAACAACACATGTTCCTTAAATTAA
Protein sequence

>MS.gene030486.t1

MVVDNLEKGEQVTQKAVTSMEKKRRIRNKTSLSCPLSKDVSKEGRSKKQKKSSGEGMKGEEKKKEEKKDQKKNIEEVPTGYIHVRARRGQATDSHSLAERVRREKISERMKILQQLVPGCDRVTGKALVLDEIINYVQSLQNQVEFLSMKLASVNPIFFDFAMDLDTLLVRPDHQRLNSITSPSTPIPHVPLCSSNHATPFADTITTSFHPVNNDYVLDYPTSIFLQGQRSNTFFEHTDGPFWDVEEQRQKLLNPYGFGNNTCSLN