AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030612

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030612.t1 MTR_8g093790 91.892 185 8 3 1 178 1 185 3.09e-119 335
MS.gene030612.t1 MTR_3g096400 52.195 205 65 9 7 178 4 208 3.56e-58 181
MS.gene030612.t1 MTR_4g094302 46.032 189 74 6 10 178 11 191 3.34e-49 157
MS.gene030612.t1 MTR_4g094302 49.167 120 51 3 10 119 11 130 7.76e-36 122
MS.gene030612.t1 MTR_2g080010 37.569 181 80 5 10 157 15 195 6.91e-31 115
MS.gene030612.t1 MTR_4g081870 38.021 192 92 7 2 169 1 189 7.46e-31 113
MS.gene030612.t1 MTR_5g041940 37.778 180 87 6 8 162 1 180 1.32e-29 109
MS.gene030612.t1 MTR_8g094580 42.675 157 67 6 5 138 2 158 4.61e-29 108
MS.gene030612.t1 MTR_2157s0010 34.946 186 88 6 4 157 2 186 1.06e-28 107
MS.gene030612.t1 MTR_7g005280 40.854 164 72 7 8 150 13 172 1.64e-28 106
MS.gene030612.t1 MTR_2g093810 36.842 171 82 5 3 147 7 177 2.36e-27 105
MS.gene030612.t1 MTR_3g088110 36.872 179 91 6 6 162 3 181 3.43e-27 103
MS.gene030612.t1 MTR_7g100990 40.252 159 69 6 6 138 7 165 4.40e-27 104
MS.gene030612.t1 MTR_4g089135 37.853 177 65 6 10 141 15 191 2.87e-26 102
MS.gene030612.t1 MTR_8g059170 40.854 164 67 7 6 143 10 169 2.97e-26 102
MS.gene030612.t1 MTR_2g079990 39.241 158 76 5 6 143 12 169 5.08e-26 102
MS.gene030612.t1 MTR_3g435150 37.423 163 80 5 10 150 16 178 2.27e-25 99.4
MS.gene030612.t1 MTR_3g096920 39.103 156 73 6 5 138 2 157 3.78e-25 98.6
MS.gene030612.t1 MTR_7g085220 37.647 170 72 7 2 138 1 169 4.31e-25 99.4
MS.gene030612.t1 MTR_6g084430 38.667 150 74 5 10 141 5 154 1.29e-24 97.8
MS.gene030612.t1 MTR_4g108760 39.073 151 70 5 10 138 17 167 1.98e-24 97.8
MS.gene030612.t1 MTR_3g109340 36.905 168 86 5 10 157 22 189 3.26e-23 94.7
MS.gene030612.t1 MTR_1g008740 40.397 151 68 5 10 138 27 177 4.58e-23 94.4
MS.gene030612.t1 MTR_3g109340 36.095 169 87 5 10 157 22 190 5.23e-23 94.0
MS.gene030612.t1 MTR_1g008740 40.397 151 68 5 10 138 13 163 6.63e-23 93.6
MS.gene030612.t1 MTR_4g035590 33.508 191 102 8 4 169 2 192 1.17e-22 93.2
MS.gene030612.t1 MTR_7g085260 32.836 201 101 6 3 177 10 202 3.69e-22 92.0
MS.gene030612.t1 MTR_2g064470 37.662 154 71 7 8 139 9 159 4.67e-22 90.9
MS.gene030612.t1 MTR_2g079990 33.333 186 76 5 6 143 12 197 6.50e-22 91.3
MS.gene030612.t1 MTR_8g099750 34.810 158 78 5 9 141 13 170 6.75e-22 89.0
MS.gene030612.t1 MTR_3g098810 36.420 162 75 6 9 142 13 174 7.85e-22 89.0
MS.gene030612.t1 MTR_6g477900 37.821 156 75 6 6 139 2 157 8.76e-22 88.6
MS.gene030612.t1 MTR_3g064580 38.312 154 72 6 8 138 4 157 8.80e-22 92.0
MS.gene030612.t1 MTR_3g096920 39.394 132 65 4 5 121 2 133 1.11e-21 90.1
MS.gene030612.t1 MTR_1g069805 36.538 156 81 5 8 145 8 163 1.18e-21 89.0
MS.gene030612.t1 MTR_2g062730 35.057 174 88 8 1 149 1 174 1.33e-21 90.1
MS.gene030612.t1 MTR_3g070030 36.471 170 82 7 8 154 17 183 4.50e-21 88.6
MS.gene030612.t1 MTR_6g012670 33.728 169 90 5 4 150 14 182 4.86e-21 88.2
MS.gene030612.t1 MTR_7g097090 37.415 147 74 5 8 136 8 154 8.34e-21 87.0
MS.gene030612.t1 MTR_1g090720 34.810 158 75 6 10 139 42 199 1.81e-20 87.8
MS.gene030612.t1 MTR_2g078700 38.312 154 71 6 10 139 23 176 3.59e-20 86.7
MS.gene030612.t1 MTR_2g064090 32.418 182 98 8 6 162 2 183 5.35e-20 85.5
MS.gene030612.t1 MTR_5g021710 33.333 192 96 9 10 173 8 195 6.84e-20 85.5
MS.gene030612.t1 MTR_4g036030 34.197 193 98 9 10 178 7 194 6.89e-20 85.5
MS.gene030612.t1 MTR_5g076850 37.179 156 74 7 6 138 2 156 7.91e-20 86.3
MS.gene030612.t1 MTR_3g098810 39.695 131 61 4 9 121 13 143 1.13e-19 81.6
MS.gene030612.t1 MTR_8g076110 35.152 165 82 8 10 151 6 168 1.83e-19 84.0
MS.gene030612.t1 MTR_5g012080 34.839 155 78 6 10 141 7 161 2.33e-19 84.0
MS.gene030612.t1 MTR_6g011860 34.146 164 74 7 10 141 15 176 4.57e-19 83.6
MS.gene030612.t1 MTR_3g070040 37.500 152 74 6 8 138 6 157 6.12e-19 82.8
MS.gene030612.t1 MTR_2g068880 30.723 166 79 6 12 141 24 189 1.29e-18 81.6
MS.gene030612.t1 MTR_1g096430 36.478 159 76 8 2 138 1 156 4.66e-18 80.1
MS.gene030612.t1 MTR_2g068920 30.357 168 81 6 12 143 23 190 5.07e-18 79.7
MS.gene030612.t1 MTR_6g032770 35.000 160 80 5 4 139 12 171 5.80e-18 80.5
MS.gene030612.t1 MTR_8g467490 32.530 166 80 7 12 145 16 181 6.22e-18 80.5
MS.gene030612.t1 MTR_4g134460 36.242 149 70 6 12 138 56 201 6.57e-18 80.5
MS.gene030612.t1 MTR_5g040420 33.333 165 79 7 12 145 16 180 6.94e-18 80.1
MS.gene030612.t1 MTR_3g096140 37.584 149 71 8 12 138 9 157 1.08e-17 78.6
MS.gene030612.t1 MTR_4g101680 33.987 153 80 6 10 141 7 159 1.37e-17 79.3
MS.gene030612.t1 MTR_7g011120 35.000 160 75 6 8 138 6 165 1.50e-17 78.6
MS.gene030612.t1 MTR_3g096140 37.584 149 71 8 12 138 9 157 2.33e-17 78.6
MS.gene030612.t1 MTR_3g116070 37.748 151 72 5 10 141 23 170 2.58e-17 78.2
MS.gene030612.t1 MTR_8g024480 31.481 162 79 7 9 138 14 175 4.23e-17 78.2
MS.gene030612.t1 MTR_2g086880 36.111 144 66 8 12 135 20 157 4.33e-17 78.2
MS.gene030612.t1 MTR_5g090970 28.659 164 85 5 12 143 20 183 4.81e-17 77.0
MS.gene030612.t1 MTR_5g014300 33.537 164 80 7 2 139 1 161 5.70e-17 77.4
MS.gene030612.t1 MTR_4g098630 32.044 181 98 7 8 163 4 184 6.01e-17 77.0
MS.gene030612.t1 MTR_8g023840 34.932 146 74 6 10 135 11 155 9.19e-17 77.0
MS.gene030612.t1 MTR_8g023930 31.548 168 91 8 10 157 24 187 1.45e-16 76.6
MS.gene030612.t1 MTR_2g086880 37.594 133 61 7 12 124 20 150 1.62e-16 76.6
MS.gene030612.t1 MTR_7g011130 34.337 166 74 6 8 138 17 182 2.67e-16 75.5
MS.gene030612.t1 MTR_4g075980 34.545 165 76 8 12 149 17 176 4.42e-16 74.3
MS.gene030612.t1 MTR_5g090970 28.659 164 84 6 12 143 20 182 7.07e-16 73.9
MS.gene030612.t1 MTR_8g093580 32.895 152 80 7 9 138 5 156 2.75e-15 73.2
MS.gene030612.t1 MTR_5g069030 33.766 154 76 7 9 138 24 175 5.67e-15 72.4
MS.gene030612.t1 MTR_8g063550 32.903 155 76 8 9 138 5 156 9.71e-15 71.2
MS.gene030612.t1 MTR_8g063550 32.903 155 76 8 9 138 5 156 1.34e-14 70.9
MS.gene030612.t1 MTR_3g093050 33.766 154 76 7 10 139 22 173 1.98e-14 70.5
MS.gene030612.t1 MTR_8g063550 32.903 155 76 8 9 138 5 156 2.20e-14 70.1
MS.gene030612.t1 MTR_3g093050 33.766 154 76 7 10 139 22 173 2.22e-14 70.5
MS.gene030612.t1 MTR_8g023860 31.507 146 79 6 10 135 12 156 3.97e-14 69.7
MS.gene030612.t1 MTR_3g093040 31.250 160 88 6 2 139 100 259 1.20e-13 67.8
MS.gene030612.t1 MTR_8g023900 29.560 159 88 7 13 151 196 350 1.78e-13 67.8
MS.gene030612.t1 MTR_8g023880 31.928 166 78 6 5 138 2 164 2.64e-13 66.6
MS.gene030612.t1 MTR_1g097300 34.127 126 65 5 12 119 21 146 5.06e-12 63.5
MS.gene030612.t1 MTR_4g052620 32.051 156 77 8 10 136 5 160 4.29e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030612.t1 AT5G64530 46.970 198 66 7 8 177 1 187 1.82e-51 163
MS.gene030612.t1 AT5G64530 51.261 119 50 3 8 119 1 118 1.73e-36 124
MS.gene030612.t1 AT1G61110 42.515 167 63 6 10 143 16 182 8.36e-31 114
MS.gene030612.t1 AT1G77450 41.875 160 71 6 1 138 1 160 7.64e-29 107
MS.gene030612.t1 AT2G33480 41.026 156 71 6 8 145 13 165 2.67e-28 106
MS.gene030612.t1 AT2G33480 41.139 158 70 7 8 145 13 167 3.50e-28 106
MS.gene030612.t1 AT1G69490 37.766 188 85 7 2 165 1 180 6.21e-28 105
MS.gene030612.t1 AT1G01720 42.581 155 66 6 8 139 5 159 3.59e-27 103
MS.gene030612.t1 AT3G04070 37.912 182 79 8 1 150 1 180 1.09e-26 103
MS.gene030612.t1 AT5G39610 39.130 161 76 5 1 139 11 171 1.62e-26 102
MS.gene030612.t1 AT3G18400 38.065 155 77 5 10 145 5 159 7.08e-26 101
MS.gene030612.t1 AT1G52890 36.686 169 83 6 6 154 10 174 9.42e-26 100
MS.gene030612.t1 AT3G12977 34.302 172 90 6 8 158 16 185 1.19e-25 99.8
MS.gene030612.t1 AT3G15500 35.673 171 90 5 6 156 10 180 2.36e-25 99.8
MS.gene030612.t1 AT5G63790 39.873 158 71 6 5 138 45 202 3.24e-25 99.4
MS.gene030612.t1 AT5G63790 39.873 158 71 6 5 138 55 212 4.10e-25 99.4
MS.gene030612.t1 AT2G24430 40.667 150 68 5 10 138 16 165 4.29e-25 99.0
MS.gene030612.t1 AT2G24430 40.667 150 68 5 10 138 16 165 4.29e-25 99.0
MS.gene030612.t1 AT5G13180 38.608 158 67 7 8 141 12 163 4.48e-25 97.8
MS.gene030612.t1 AT5G61430 38.217 157 75 5 5 139 11 167 5.89e-25 99.0
MS.gene030612.t1 AT4G27410 36.500 200 95 9 6 174 10 208 8.39e-25 97.8
MS.gene030612.t1 AT5G07680 39.735 151 69 5 10 138 17 167 8.70e-25 98.6
MS.gene030612.t1 AT5G07680 39.735 151 69 5 10 138 3 153 9.12e-25 98.2
MS.gene030612.t1 AT3G15510 33.333 195 91 6 14 175 21 209 1.60e-24 98.2
MS.gene030612.t1 AT3G04070 34.848 198 79 8 1 150 1 196 2.74e-24 97.4
MS.gene030612.t1 AT5G18270 38.125 160 76 5 3 139 14 173 3.33e-24 97.1
MS.gene030612.t1 AT3G04060 36.250 160 79 5 3 139 13 172 3.92e-24 96.7
MS.gene030612.t1 AT5G18270 37.500 160 77 5 3 139 14 173 9.25e-24 95.9
MS.gene030612.t1 AT5G08790 39.623 159 72 6 5 139 2 160 1.22e-23 94.7
MS.gene030612.t1 AT1G56010 35.897 156 76 6 8 139 17 172 1.52e-23 95.1
MS.gene030612.t1 AT3G17730 38.462 156 74 6 6 139 2 157 9.60e-23 91.7
MS.gene030612.t1 AT4G27410 34.234 222 96 11 6 178 10 230 4.57e-22 90.9
MS.gene030612.t1 AT5G53950 36.782 174 85 7 10 158 17 190 4.98e-22 91.7
MS.gene030612.t1 AT3G10480 37.821 156 75 7 6 139 23 178 5.59e-22 92.0
MS.gene030612.t1 AT4G10350 37.500 160 77 7 2 138 1 160 7.09e-22 90.9
MS.gene030612.t1 AT3G10480 37.580 157 75 7 6 139 23 179 7.70e-22 91.7
MS.gene030612.t1 AT1G79580 36.471 170 81 8 2 144 3 172 1.52e-21 90.1
MS.gene030612.t1 AT1G79580 36.471 170 81 8 2 144 3 172 1.52e-21 90.1
MS.gene030612.t1 AT1G79580 36.471 170 81 8 2 144 3 172 1.52e-21 90.1
MS.gene030612.t1 AT1G79580 36.471 170 81 8 2 144 3 172 1.52e-21 90.1
MS.gene030612.t1 AT1G79580 36.471 170 81 8 2 144 3 172 1.52e-21 90.1
MS.gene030612.t1 AT3G29035 42.188 128 58 4 8 119 22 149 2.05e-21 89.4
MS.gene030612.t1 AT3G15170 37.013 154 73 5 10 139 20 173 2.16e-21 89.0
MS.gene030612.t1 AT3G10490 35.256 156 78 7 7 139 24 179 2.58e-21 87.4
MS.gene030612.t1 AT1G33280 36.774 155 78 6 4 138 2 156 4.17e-21 88.2
MS.gene030612.t1 AT1G76420 36.196 163 76 7 1 138 12 171 7.23e-21 87.8
MS.gene030612.t1 AT5G62380 33.962 159 82 7 5 141 2 159 1.32e-20 87.4
MS.gene030612.t1 AT5G62380 33.962 159 82 7 5 141 2 159 1.32e-20 87.4
MS.gene030612.t1 AT1G52880 37.500 160 68 6 14 141 21 180 1.50e-20 87.0
MS.gene030612.t1 AT3G10490 35.256 156 78 7 7 139 24 179 2.17e-20 87.4
MS.gene030612.t1 AT5G17260 35.897 156 77 6 6 138 2 157 3.23e-20 87.0
MS.gene030612.t1 AT3G03200 35.714 154 76 6 8 138 4 157 8.29e-20 85.9
MS.gene030612.t1 AT2G18060 33.129 163 88 6 7 148 6 168 1.41e-19 84.7
MS.gene030612.t1 AT2G18060 33.129 163 88 6 7 148 6 168 1.41e-19 84.7
MS.gene030612.t1 AT2G43000 31.410 156 86 5 12 146 20 175 1.61e-19 83.6
MS.gene030612.t1 AT1G65910 37.255 153 74 6 8 138 4 156 3.30e-19 84.3
MS.gene030612.t1 AT1G54330 35.526 152 79 6 8 140 4 155 3.45e-19 82.8
MS.gene030612.t1 AT3G61910 32.934 167 76 7 9 139 10 176 3.68e-19 83.2
MS.gene030612.t1 AT4G36160 33.529 170 91 7 1 148 5 174 3.73e-19 83.6
MS.gene030612.t1 AT4G36160 33.529 170 91 7 1 148 1 170 4.14e-19 83.6
MS.gene030612.t1 AT4G36160 33.529 170 91 7 1 148 1 170 4.14e-19 83.6
MS.gene030612.t1 AT5G04410 35.583 163 77 7 2 139 1 160 7.11e-19 83.6
MS.gene030612.t1 AT4G17980 35.366 164 77 7 2 139 1 161 2.88e-18 79.7
MS.gene030612.t1 AT4G17980 35.366 164 77 7 2 139 1 161 3.09e-18 80.1
MS.gene030612.t1 AT5G46590 35.185 162 78 7 2 139 1 159 4.04e-18 80.1
MS.gene030612.t1 AT4G28530 33.939 165 73 6 9 138 9 172 4.32e-18 80.5
MS.gene030612.t1 AT3G10500 35.152 165 79 7 2 141 1 162 5.97e-18 80.9
MS.gene030612.t1 AT1G32770 30.675 163 80 6 9 138 15 177 6.14e-18 80.1
MS.gene030612.t1 AT1G62700 34.839 155 78 7 9 141 6 159 7.98e-18 80.1
MS.gene030612.t1 AT1G62700 34.839 155 78 7 9 141 6 159 7.98e-18 80.1
MS.gene030612.t1 AT3G10480 32.105 190 73 7 6 139 23 212 9.68e-18 80.1
MS.gene030612.t1 AT3G12910 30.682 176 83 9 5 143 17 190 1.14e-17 79.0
MS.gene030612.t1 AT1G12260 34.615 156 75 8 10 141 7 159 1.24e-17 79.3
MS.gene030612.t1 AT5G66300 33.333 159 80 7 7 141 9 165 1.47e-17 78.6
MS.gene030612.t1 AT4G35580 34.161 161 80 7 2 138 1 159 1.93e-17 79.3
MS.gene030612.t1 AT1G12260 34.615 156 75 8 10 141 7 159 2.12e-17 79.0
MS.gene030612.t1 AT4G35580 34.161 161 80 7 2 138 1 159 2.65e-17 79.0
MS.gene030612.t1 AT4G35580 34.161 161 80 7 2 138 1 159 2.68e-17 79.0
MS.gene030612.t1 AT1G71930 32.903 155 83 6 10 143 9 163 4.09e-17 77.4
MS.gene030612.t1 AT1G71930 32.903 155 83 6 10 143 9 163 4.09e-17 77.4
MS.gene030612.t1 AT1G33060 33.333 153 76 7 10 138 24 174 4.19e-17 78.6
MS.gene030612.t1 AT1G33060 33.333 153 76 7 10 138 24 174 4.65e-17 78.2
MS.gene030612.t1 AT5G39820 31.395 172 71 7 12 138 22 191 6.37e-17 77.0
MS.gene030612.t1 AT2G02450 34.228 149 73 6 12 138 53 198 1.04e-16 77.0
MS.gene030612.t1 AT1G26870 33.125 160 76 6 10 138 16 175 1.12e-16 77.0
MS.gene030612.t1 AT2G02450 34.228 149 73 6 12 138 53 198 1.26e-16 76.6
MS.gene030612.t1 AT2G17040 35.714 154 69 7 12 141 8 155 3.07e-16 74.7
MS.gene030612.t1 AT5G46590 36.232 138 66 6 2 120 1 135 5.45e-16 73.9
MS.gene030612.t1 AT1G32510 34.375 160 75 7 10 139 6 165 1.00e-15 73.2
MS.gene030612.t1 AT1G02230 32.414 145 78 5 11 135 4 148 1.18e-15 73.9
MS.gene030612.t1 AT2G46770 29.825 171 84 7 9 143 15 185 2.15e-15 73.2
MS.gene030612.t1 AT5G50820 34.058 138 73 4 6 128 13 147 6.47e-15 69.7
MS.gene030612.t1 AT1G34190 32.584 178 89 8 12 158 18 195 1.66e-14 70.9
MS.gene030612.t1 AT3G04420 33.108 148 78 6 11 138 4 150 2.82e-14 69.3
MS.gene030612.t1 AT3G04420 33.103 145 76 6 11 135 4 147 3.00e-14 69.7
MS.gene030612.t1 AT4G01520 32.468 154 80 6 8 138 2 154 5.21e-14 68.9
MS.gene030612.t1 AT1G02250 31.034 145 80 4 11 135 4 148 5.75e-14 68.9
MS.gene030612.t1 AT4G01540 32.886 149 76 6 13 138 7 154 7.96e-14 68.9
MS.gene030612.t1 AT4G01540 32.886 149 76 6 13 138 7 154 9.03e-14 68.6
MS.gene030612.t1 AT4G01540 32.877 146 74 6 13 135 7 151 1.07e-13 68.6
MS.gene030612.t1 AT3G49530 29.167 168 92 7 10 152 13 178 1.28e-13 68.2
MS.gene030612.t1 AT4G01540 32.886 149 76 6 13 138 7 154 1.94e-13 67.8
MS.gene030612.t1 AT3G49530 29.167 168 92 7 10 152 13 178 1.99e-13 67.8
MS.gene030612.t1 AT1G34180 29.688 192 102 9 12 178 18 201 2.68e-12 64.7
MS.gene030612.t1 AT1G34180 34.921 126 64 5 12 119 18 143 3.85e-12 63.9
MS.gene030612.t1 AT1G56010 37.634 93 44 4 61 139 13 105 1.35e-11 61.6
MS.gene030612.t1 AT5G61430 38.144 97 39 4 64 139 8 104 5.09e-11 60.1
MS.gene030612.t1 AT2G33480 38.614 101 43 5 64 145 10 110 5.17e-11 59.3
MS.gene030612.t1 AT5G64060 30.921 152 83 7 10 139 6 157 7.60e-11 60.1

Find 46 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAAACCTATAAATAAAT+TGG 0.146052 4.3:+8084333 MS.gene030612:CDS
TTCATTCCCAATTTATTTAT+AGG 0.164515 4.3:-8084340 None:intergenic
AATGGATATTGGAAGGAAAT+TGG 0.199332 4.3:+8084307 MS.gene030612:CDS
AAGCTAATTATTAACTAATT+AGG 0.204594 4.3:-8084761 None:intergenic
GAAAATAGAAGCACTGAAAA+TGG 0.301344 4.3:+8084289 MS.gene030612:CDS
ATGGATATTGGAAGGAAATT+GGG 0.330470 4.3:+8084308 MS.gene030612:CDS
GTGTGAGACAGGATCATAAT+TGG 0.363529 4.3:+8084591 MS.gene030612:intron
GAAGCACTGAAAATGGATAT+TGG 0.365813 4.3:+8084296 MS.gene030612:CDS
TTGATCAAGATCATCATCTA+AGG 0.370976 4.3:-8084729 None:intergenic
TGGTGAGACAAGTGAAGTTC+AGG 0.398752 4.3:-8083593 None:intergenic
CATGATCCTTGGGAAATATA+CGG 0.400748 4.3:+8083614 MS.gene030612:CDS
AGTACCTTGTATTCAACCTT+GGG 0.405124 4.3:+8084365 MS.gene030612:CDS
ACTTGTCTCACCATGATCCT+TGG 0.405810 4.3:+8083603 MS.gene030612:CDS
CTTGTCTCACCATGATCCTT+GGG 0.421667 4.3:+8083604 MS.gene030612:CDS
GGTATGATGCTAGGATGATA+TGG 0.434256 4.3:-8083572 None:intergenic
GATGAGGATATTGATGATAA+TGG 0.434866 4.3:+8084671 MS.gene030612:CDS
CTTCCCCAAGGTTGAATACA+AGG 0.441845 4.3:-8084369 None:intergenic
ACAAAACCTATAAATAAATT+GGG 0.452946 4.3:+8084334 MS.gene030612:CDS
AGCAGCGTGAAGCTTCCTCC+TGG 0.455992 4.3:+8083485 MS.gene030612:CDS
AATTGAAACAAGTTGGGTAA+TGG 0.457936 4.3:+8084393 MS.gene030612:CDS
GGGAAGGAATTGAAACAAGT+TGG 0.476317 4.3:+8084386 MS.gene030612:CDS
ATGATATGGTAAAGAAGCCT+TGG 0.489756 4.3:-8083558 None:intergenic
CGTATATTTCCCAAGGATCA+TGG 0.491411 4.3:-8083613 None:intergenic
GGAAGGAATTGAAACAAGTT+GGG 0.495348 4.3:+8084387 MS.gene030612:CDS
ATATCATATTTCCTCATCTC+CGG 0.517814 4.3:+8084420 MS.gene030612:CDS
AGGACTAGTTCTTCATCCGT+TGG 0.520052 4.3:-8083521 None:intergenic
AGGATCATAATTGGAGCAGA+TGG 0.520438 4.3:+8084600 MS.gene030612:intron
TAATTATTAACTAATTAGGT+AGG 0.520877 4.3:-8084757 None:intergenic
TGGATATTGGAAGGAAATTG+GGG 0.532640 4.3:+8084309 MS.gene030612:CDS
AAGTACCTTGTATTCAACCT+TGG 0.541631 4.3:+8084364 MS.gene030612:CDS
CTTGTATTCAACCTTGGGGA+AGG 0.542660 4.3:+8084370 MS.gene030612:CDS
GCAGCGTGAAGCTTCCTCCT+GGG 0.544030 4.3:+8083486 MS.gene030612:CDS
GAAAAGAATATGTCACATCA+AGG 0.553106 4.3:+8084641 MS.gene030612:CDS
TGAAGTTCAGGTATGATGCT+AGG 0.561302 4.3:-8083581 None:intergenic
TTGTTTCAATTCCTTCCCCA+AGG 0.571160 4.3:-8084381 None:intergenic
GGATCATAATTGGAGCAGAT+GGG 0.571776 4.3:+8084601 MS.gene030612:intron
CCTTGGAATAGAGAAAATGA+AGG 0.572117 4.3:-8083541 None:intergenic
CACTGAAAATGGATATTGGA+AGG 0.575605 4.3:+8084300 MS.gene030612:CDS
ACAATCACTTACCGGAGATG+AGG 0.598757 4.3:-8084431 None:intergenic
GACATACCGTATATTTCCCA+AGG 0.621341 4.3:-8083620 None:intergenic
ACAGGTAAAGCATTGTCAAG+TGG 0.622513 4.3:+8084235 MS.gene030612:intron
ACATCAAGGTGCAAGCGATG+AGG 0.654589 4.3:+8084655 MS.gene030612:CDS
GTACCTTGTATTCAACCTTG+GGG 0.661844 4.3:+8084366 MS.gene030612:CDS
ATAATGGAACAGATGTGTCA+TGG 0.669182 4.3:+8084687 MS.gene030612:CDS
GTTGGATCAAAGTAAAACCC+AGG 0.688035 4.3:-8083503 None:intergenic
GGATCAAAGTAAAACCCAGG+AGG 0.734981 4.3:-8083500 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTATTTTATTTTTTACC+CGG - chr4.3:8084099-8084118 None:intergenic 10.0%
!!! TTTTCTTTTAAAATAGAATA+TGG + chr4.3:8083992-8084011 MS.gene030612:intron 10.0%
!! ACAAAACCTATAAATAAATT+GGG + chr4.3:8084334-8084353 MS.gene030612:CDS 15.0%
!! ATTCTAAATACGATTTAAAT+TGG + chr4.3:8083864-8083883 MS.gene030612:intron 15.0%
!! TATAATTAATCTATAAGTGA+TGG - chr4.3:8084055-8084074 None:intergenic 15.0%
!! TTCTAAATACGATTTAAATT+GGG + chr4.3:8083865-8083884 MS.gene030612:intron 15.0%
!!! TAATTTTTTTCCACATTAAT+TGG - chr4.3:8084522-8084541 None:intergenic 15.0%
!! AAATTTATGTAAAACCTATC+TGG + chr4.3:8084144-8084163 MS.gene030612:intron 20.0%
!! ATTAGCAATAACATATGTTA+AGG - chr4.3:8083910-8083929 None:intergenic 20.0%
!! CACAAAACCTATAAATAAAT+TGG + chr4.3:8084333-8084352 MS.gene030612:CDS 20.0%
!! GTGTTAAAGACAAAATAAAT+TGG - chr4.3:8083767-8083786 None:intergenic 20.0%
!! TAAAGACAAAATAAATTGGT+AGG - chr4.3:8083763-8083782 None:intergenic 20.0%
!! TTCATTCCCAATTTATTTAT+AGG - chr4.3:8084343-8084362 None:intergenic 20.0%
!!! ATTTGTTGTGATTTATGAAT+AGG - chr4.3:8084475-8084494 None:intergenic 20.0%
!!! CATAGCATATAATTGAATTT+TGG - chr4.3:8083967-8083986 None:intergenic 20.0%
!!! GATAGGTTTTACATAAATTT+TGG - chr4.3:8084144-8084163 None:intergenic 20.0%
!!! TCAAAGTTTTATCTAAAATC+CGG + chr4.3:8084077-8084096 MS.gene030612:intron 20.0%
! TCTATATATGCATGCATATA+TGG - chr4.3:8083728-8083747 None:intergenic 25.0%
!! ATTAATCTATAAGTGATGGA+TGG - chr4.3:8084051-8084070 None:intergenic 25.0%
!!! CAAAGTTTTATCTAAAATCC+GGG + chr4.3:8084078-8084097 MS.gene030612:intron 25.0%
AACAAAACACAATCACTTAC+CGG - chr4.3:8084442-8084461 None:intergenic 30.0%
AATCGTACTCTTTATAGTTG+TGG + chr4.3:8084169-8084188 MS.gene030612:intron 30.0%
AATGCTTTACCTGTTTACAA+AGG - chr4.3:8084229-8084248 None:intergenic 30.0%
AATGGATATTGGAAGGAAAT+TGG + chr4.3:8084307-8084326 MS.gene030612:CDS 30.0%
ATATACATGCCTTTGTAAAC+AGG + chr4.3:8084217-8084236 MS.gene030612:intron 30.0%
ATATCATATTTCCTCATCTC+CGG + chr4.3:8084420-8084439 MS.gene030612:CDS 30.0%
ATGGATATTGGAAGGAAATT+GGG + chr4.3:8084308-8084327 MS.gene030612:CDS 30.0%
ATTCAATGATCGATTAATCC+CGG + chr4.3:8084015-8084034 MS.gene030612:intron 30.0%
ATTGATCAAGTGACATATAC+TGG + chr4.3:8083690-8083709 MS.gene030612:intron 30.0%
CAAATCATGACCAATTAATG+TGG + chr4.3:8084509-8084528 MS.gene030612:intron 30.0%
GAAAAGAATATGTCACATCA+AGG + chr4.3:8084641-8084660 MS.gene030612:CDS 30.0%
TAACATATGTTAAGGTCGAA+AGG - chr4.3:8083902-8083921 None:intergenic 30.0%
TTGATCAAGATCATCATCTA+AGG - chr4.3:8084732-8084751 None:intergenic 30.0%
! AAAGGCATGTATATTTCTTG+GGG - chr4.3:8084211-8084230 None:intergenic 30.0%
! AATTGAAACAAGTTGGGTAA+TGG + chr4.3:8084393-8084412 MS.gene030612:CDS 30.0%
! ACAAAGGCATGTATATTTCT+TGG - chr4.3:8084213-8084232 None:intergenic 30.0%
! CAAAGGCATGTATATTTCTT+GGG - chr4.3:8084212-8084231 None:intergenic 30.0%
! GATGAGGATATTGATGATAA+TGG + chr4.3:8084671-8084690 MS.gene030612:CDS 30.0%
!! GAAAATAGAAGCACTGAAAA+TGG + chr4.3:8084289-8084308 MS.gene030612:CDS 30.0%
!! TATTTTTGATTGTGTGAGAC+AGG + chr4.3:8084580-8084599 MS.gene030612:intron 30.0%
!!! CTTACTTTCTAGCTGAATTT+TGG - chr4.3:8083821-8083840 None:intergenic 30.0%
AAGGCATGTATATTTCTTGG+GGG - chr4.3:8084210-8084229 None:intergenic 35.0%
AAGTACCTTGTATTCAACCT+TGG + chr4.3:8084364-8084383 MS.gene030612:CDS 35.0%
ACGGTATGTCTTATAGTACT+AGG + chr4.3:8083633-8083652 MS.gene030612:intron 35.0%
AGTACCTTGTATTCAACCTT+GGG + chr4.3:8084365-8084384 MS.gene030612:CDS 35.0%
ATGATATGGTAAAGAAGCCT+TGG - chr4.3:8083561-8083580 None:intergenic 35.0%
CACTGAAAATGGATATTGGA+AGG + chr4.3:8084300-8084319 MS.gene030612:CDS 35.0%
CATGATCCTTGGGAAATATA+CGG + chr4.3:8083614-8083633 MS.gene030612:CDS 35.0%
CCTTGGAATAGAGAAAATGA+AGG - chr4.3:8083544-8083563 None:intergenic 35.0%
GGAAGGAATTGAAACAAGTT+GGG + chr4.3:8084387-8084406 MS.gene030612:CDS 35.0%
TGGATATTGGAAGGAAATTG+GGG + chr4.3:8084309-8084328 MS.gene030612:CDS 35.0%
! ATAATGGAACAGATGTGTCA+TGG + chr4.3:8084687-8084706 MS.gene030612:CDS 35.0%
! CCTTCATTTTCTCTATTCCA+AGG + chr4.3:8083541-8083560 MS.gene030612:CDS 35.0%
! GCTTCTATTTTCCTTCACTT+CGG - chr4.3:8084281-8084300 None:intergenic 35.0%
! TGGGTTTTACTTTGATCCAA+CGG + chr4.3:8083505-8083524 MS.gene030612:CDS 35.0%
!! AAGAGTACGATTTTCCAGAT+AGG - chr4.3:8084161-8084180 None:intergenic 35.0%
!! GAAGCACTGAAAATGGATAT+TGG + chr4.3:8084296-8084315 MS.gene030612:CDS 35.0%
AGGATCATAATTGGAGCAGA+TGG + chr4.3:8084600-8084619 MS.gene030612:intron 40.0%
CGTATATTTCCCAAGGATCA+TGG - chr4.3:8083616-8083635 None:intergenic 40.0%
GACATACCGTATATTTCCCA+AGG - chr4.3:8083623-8083642 None:intergenic 40.0%
GGATCATAATTGGAGCAGAT+GGG + chr4.3:8084601-8084620 MS.gene030612:intron 40.0%
GGGAAGGAATTGAAACAAGT+TGG + chr4.3:8084386-8084405 MS.gene030612:CDS 40.0%
GGTATGATGCTAGGATGATA+TGG - chr4.3:8083575-8083594 None:intergenic 40.0%
GTACCTTGTATTCAACCTTG+GGG + chr4.3:8084366-8084385 MS.gene030612:CDS 40.0%
GTGTGAGACAGGATCATAAT+TGG + chr4.3:8084591-8084610 MS.gene030612:intron 40.0%
GTTGGATCAAAGTAAAACCC+AGG - chr4.3:8083506-8083525 None:intergenic 40.0%
TGAAGTTCAGGTATGATGCT+AGG - chr4.3:8083584-8083603 None:intergenic 40.0%
TTGTTTCAATTCCTTCCCCA+AGG - chr4.3:8084384-8084403 None:intergenic 40.0%
!! ACAGGTAAAGCATTGTCAAG+TGG + chr4.3:8084235-8084254 MS.gene030612:intron 40.0%
!! TAAGTGATGGATGGTGAGAT+TGG - chr4.3:8084042-8084061 None:intergenic 40.0%
!!! CTACTTTTTCACCGAAGTGA+AGG + chr4.3:8084267-8084286 MS.gene030612:CDS 40.0%
ACAATCACTTACCGGAGATG+AGG - chr4.3:8084434-8084453 None:intergenic 45.0%
ACTTGTCTCACCATGATCCT+TGG + chr4.3:8083603-8083622 MS.gene030612:CDS 45.0%
CTTCCCCAAGGTTGAATACA+AGG - chr4.3:8084372-8084391 None:intergenic 45.0%
CTTGTATTCAACCTTGGGGA+AGG + chr4.3:8084370-8084389 MS.gene030612:CDS 45.0%
CTTGTCTCACCATGATCCTT+GGG + chr4.3:8083604-8083623 MS.gene030612:CDS 45.0%
GGATCAAAGTAAAACCCAGG+AGG - chr4.3:8083503-8083522 None:intergenic 45.0%
TGGTGAGACAAGTGAAGTTC+AGG - chr4.3:8083596-8083615 None:intergenic 45.0%
!! AGGACTAGTTCTTCATCCGT+TGG - chr4.3:8083524-8083543 None:intergenic 45.0%
ACATCAAGGTGCAAGCGATG+AGG + chr4.3:8084655-8084674 MS.gene030612:CDS 50.0%
! ATGGATGGTGAGATTGGTCC+GGG - chr4.3:8084036-8084055 None:intergenic 50.0%
! GATGGATGGTGAGATTGGTC+CGG - chr4.3:8084037-8084056 None:intergenic 50.0%
AGCAGCGTGAAGCTTCCTCC+TGG + chr4.3:8083485-8083504 MS.gene030612:CDS 60.0%
GCAGCGTGAAGCTTCCTCCT+GGG + chr4.3:8083486-8083505 MS.gene030612:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 8083470 8084769 8083470 ID=MS.gene030612
chr4.3 mRNA 8083470 8084769 8083470 ID=MS.gene030612.t1;Parent=MS.gene030612
chr4.3 exon 8083470 8083635 8083470 ID=MS.gene030612.t1.exon1;Parent=MS.gene030612.t1
chr4.3 CDS 8083470 8083635 8083470 ID=cds.MS.gene030612.t1;Parent=MS.gene030612.t1
chr4.3 exon 8084239 8084441 8084239 ID=MS.gene030612.t1.exon2;Parent=MS.gene030612.t1
chr4.3 CDS 8084239 8084441 8084239 ID=cds.MS.gene030612.t1;Parent=MS.gene030612.t1
chr4.3 exon 8084602 8084769 8084602 ID=MS.gene030612.t1.exon3;Parent=MS.gene030612.t1
chr4.3 CDS 8084602 8084769 8084602 ID=cds.MS.gene030612.t1;Parent=MS.gene030612.t1
Gene Sequence

>MS.gene030612

ATGATGGGGAGTGAAAGCAGCGTGAAGCTTCCTCCTGGGTTTTACTTTGATCCAACGGATGAAGAACTAGTCCTTCATTTTCTCTATTCCAAGGCTTCTTTACCATATCATCCTAGCATCATACCTGAACTTCACTTGTCTCACCATGATCCTTGGGAAATATACGGTATGTCTTATAGTACTAGGTATATATATTTCATATAATATTTTCAATTTCAATATTGATCAAGTGACATATACTGGATATTTAGTTGACCATATATGCATGCATATATAGATAAAACTTCATTCCTACCAATTTATTTTGTCTTTAACACTTTGATTCTCATTAAAATTAGCTCTCATAGTCCAAAATTCAGCTAGAAAGTAAGTAAGTAAAATCTAACAAAAAGATATTCTAAATACGATTTAAATTGGGATACTCTCAATCCTTTCGACCTTAACATATGTTATTGCTAATTAAAAATATAGTTTGTGATATATGTAATCTTCAGCCAAAATTCAATTATATGCTATGAGATTTTTTCTTTTAAAATAGAATATGGATTCAATGATCGATTAATCCCGGACCAATCTCACCATCCATCACTTATAGATTAATTATATATCAAAGTTTTATCTAAAATCCGGGTAAAAAATAAAATAAATATACGTTATTTGTAGAAAATTATCCAAAATTTATGTAAAACCTATCTGGAAAATCGTACTCTTTATAGTTGTGGATGTGTATACTAACACCCCCAAGAAATATACATGCCTTTGTAAACAGGTAAAGCATTGTCAAGTGGAAATGAGCACTACTTTTTCACCGAAGTGAAGGAAAATAGAAGCACTGAAAATGGATATTGGAAGGAAATTGGGGTCACAAAACCTATAAATAAATTGGGAATGAAGAAGTACCTTGTATTCAACCTTGGGGAAGGAATTGAAACAAGTTGGGTAATGGAAGAATATCATATTTCCTCATCTCCGGTAAGTGATTGTGTTTTGTTTTGTTAACTACCTATTCATAAATCACAACAAATTACTTACTAAGAAACAAATCATGACCAATTAATGTGGAAAAAAATTAACATGAAGAACATAAAATTAAATTGATTTGTATCAATTTATTTTTGATTGTGTGAGACAGGATCATAATTGGAGCAGATGGGTTTTATGCAAAGTTTATGAAAAGAATATGTCACATCAAGGTGCAAGCGATGAGGATATTGATGATAATGGAACAGATGTGTCATGGCTAGATGAAGTGTTTATGTCCTTAGATGATGATCTTGATCAAACAAGCCTACCTAATTAG
Protein sequence

>MS.gene030612.t1

MMGSESSVKLPPGFYFDPTDEELVLHFLYSKASLPYHPSIIPELHLSHHDPWEIYGKALSSGNEHYFFTEVKENRSTENGYWKEIGVTKPINKLGMKKYLVFNLGEGIETSWVMEEYHISSSPDHNWSRWVLCKVYEKNMSHQGASDEDIDDNGTDVSWLDEVFMSLDDDLDQTSLPN