AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030625

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030625.t1 MTR_8g093580 94.647 411 21 1 1 411 80 489 0.0 789
MS.gene030625.t1 MTR_8g063550 68.719 406 79 7 1 405 80 438 0.0 522
MS.gene030625.t1 MTR_8g063550 76.333 300 65 4 1 299 80 374 1.61e-156 449
MS.gene030625.t1 MTR_8g063550 81.200 250 45 2 1 250 80 327 1.37e-142 410
MS.gene030625.t1 MTR_3g096140 59.355 155 58 2 1 150 81 235 1.11e-60 198
MS.gene030625.t1 MTR_3g096140 59.355 155 58 2 1 150 60 214 7.88e-60 197
MS.gene030625.t1 MTR_3g096140 59.355 155 58 2 1 150 81 235 1.25e-59 198
MS.gene030625.t1 MTR_1g090723 55.789 95 42 0 3 97 85 179 1.63e-36 141
MS.gene030625.t1 MTR_1g090723 57.895 95 39 1 3 97 85 178 5.09e-36 139
MS.gene030625.t1 MTR_1g090723 57.895 95 39 1 3 97 85 178 6.63e-36 139
MS.gene030625.t1 MTR_1g097300 56.000 100 44 0 3 102 95 194 6.59e-34 133
MS.gene030625.t1 MTR_0036s0150 52.577 97 45 1 3 99 82 177 2.40e-33 131
MS.gene030625.t1 MTR_6g477900 54.545 77 35 0 3 79 82 158 1.93e-25 103
MS.gene030625.t1 MTR_1g090720 48.246 114 52 2 1 108 116 228 7.37e-25 106
MS.gene030625.t1 MTR_2g062730 47.368 95 43 3 3 96 87 175 4.15e-23 99.8
MS.gene030625.t1 MTR_5g076850 46.591 88 46 1 3 89 82 169 7.56e-22 98.2
MS.gene030625.t1 MTR_4g108760 46.988 83 42 1 3 83 91 173 1.39e-21 95.5
MS.gene030625.t1 MTR_7g011130 39.423 104 47 2 3 90 93 196 1.42e-21 95.5
MS.gene030625.t1 MTR_3g070040 41.489 94 48 3 3 90 79 171 1.87e-21 94.7
MS.gene030625.t1 MTR_3g064580 46.591 88 45 2 3 88 82 169 2.72e-21 96.7
MS.gene030625.t1 MTR_6g084430 50.667 75 35 1 3 77 79 151 2.86e-21 94.4
MS.gene030625.t1 MTR_4g101680 43.220 118 58 5 3 117 84 195 4.82e-21 94.0
MS.gene030625.t1 MTR_3g435150 45.783 83 43 1 3 83 90 172 5.66e-21 92.8
MS.gene030625.t1 MTR_2g064090 52.632 76 35 1 3 77 82 157 1.40e-20 92.4
MS.gene030625.t1 MTR_7g011120 39.796 98 49 2 3 90 82 179 1.70e-20 91.7
MS.gene030625.t1 MTR_4g035590 48.684 76 36 1 3 78 85 157 1.77e-20 92.4
MS.gene030625.t1 MTR_8g024480 42.353 85 40 1 3 78 92 176 2.00e-20 93.2
MS.gene030625.t1 MTR_3g109340 45.783 83 42 2 3 83 96 177 2.82e-20 91.7
MS.gene030625.t1 MTR_3g109340 44.578 83 42 2 3 83 96 176 6.90e-20 90.9
MS.gene030625.t1 MTR_3g093050 48.052 77 40 0 1 77 96 172 8.74e-20 92.0
MS.gene030625.t1 MTR_6g012670 40.000 95 51 2 3 93 94 186 8.90e-20 89.7
MS.gene030625.t1 MTR_3g093050 48.052 77 40 0 1 77 96 172 9.46e-20 92.0
MS.gene030625.t1 MTR_1g096430 46.512 86 42 2 3 87 84 166 1.07e-19 89.7
MS.gene030625.t1 MTR_5g069030 46.154 78 42 0 1 78 99 176 1.07e-19 91.7
MS.gene030625.t1 MTR_8g076110 48.684 76 37 1 3 78 83 156 3.23e-19 88.2
MS.gene030625.t1 MTR_2g078700 48.810 84 37 3 3 81 97 179 3.63e-19 89.0
MS.gene030625.t1 MTR_4g036030 39.623 106 51 4 3 104 84 180 9.37e-19 87.4
MS.gene030625.t1 MTR_5g012080 36.486 148 74 7 3 147 84 214 1.14e-18 87.0
MS.gene030625.t1 MTR_7g005280 42.529 87 46 2 3 87 86 170 2.12e-18 84.7
MS.gene030625.t1 MTR_3g070030 39.362 94 49 3 3 90 93 184 2.47e-18 86.3
MS.gene030625.t1 MTR_2g064470 47.368 76 39 1 3 77 83 158 2.87e-18 85.1
MS.gene030625.t1 MTR_6g032770 48.750 80 35 3 3 77 92 170 3.59e-18 85.9
MS.gene030625.t1 MTR_3g093040 45.570 79 39 2 1 77 182 258 7.23e-18 84.3
MS.gene030625.t1 MTR_5g021710 46.053 76 39 1 3 78 85 158 8.40e-18 84.3
MS.gene030625.t1 MTR_1g008740 45.455 77 39 2 3 77 88 163 9.22e-18 84.3
MS.gene030625.t1 MTR_1g008740 45.455 77 39 2 3 77 102 177 1.34e-17 84.0
MS.gene030625.t1 MTR_2g079990 35.789 95 58 1 3 94 90 184 1.85e-17 83.6
MS.gene030625.t1 MTR_7g085260 45.783 83 35 3 3 77 91 171 2.41e-17 83.6
MS.gene030625.t1 MTR_4g081870 39.759 83 46 2 3 81 83 165 6.56e-17 80.9
MS.gene030625.t1 MTR_2g086880 43.836 73 34 1 2 74 92 157 1.17e-16 82.0
MS.gene030625.t1 MTR_2g086880 43.836 73 34 1 2 74 92 157 1.44e-16 82.0
MS.gene030625.t1 MTR_3g088110 42.500 80 42 2 3 79 81 159 3.33e-16 79.0
MS.gene030625.t1 MTR_4g098630 40.476 84 47 1 3 83 82 165 4.81e-16 79.0
MS.gene030625.t1 MTR_8g099750 38.542 96 48 3 1 89 84 175 7.63e-16 76.6
MS.gene030625.t1 MTR_5g041940 40.000 80 44 2 3 78 77 156 1.37e-15 76.6
MS.gene030625.t1 MTR_4g075980 47.436 78 33 2 3 77 89 161 1.87e-15 77.0
MS.gene030625.t1 MTR_8g094580 38.272 81 45 2 3 79 27 106 1.98e-15 75.5
MS.gene030625.t1 MTR_2157s0010 36.957 92 46 2 3 82 82 173 1.98e-15 76.3
MS.gene030625.t1 MTR_1g069805 45.455 77 36 2 3 77 84 156 2.00e-15 76.3
MS.gene030625.t1 MTR_7g100990 37.363 91 50 3 3 87 85 174 2.02e-15 77.0
MS.gene030625.t1 MTR_2g080010 38.596 114 52 5 3 103 89 197 2.32e-15 77.4
MS.gene030625.t1 MTR_4g134460 41.250 80 40 3 3 79 128 203 2.45e-15 77.8
MS.gene030625.t1 MTR_8g467490 39.535 86 41 3 3 77 88 173 2.94e-15 77.4
MS.gene030625.t1 MTR_8g059170 39.241 79 42 2 3 77 88 164 3.60e-15 76.6
MS.gene030625.t1 MTR_8g094580 38.272 81 45 2 3 79 81 160 4.44e-15 75.5
MS.gene030625.t1 MTR_7g085220 36.364 88 43 2 3 77 82 169 8.24e-15 75.5
MS.gene030625.t1 MTR_3g116070 40.506 79 45 1 3 79 98 176 1.15e-14 75.1
MS.gene030625.t1 MTR_5g014300 37.500 104 55 2 3 99 82 182 1.22e-14 75.1
MS.gene030625.t1 MTR_7g097090 44.737 76 36 2 3 76 84 155 1.30e-14 74.3
MS.gene030625.t1 MTR_6g011860 40.000 85 41 3 3 77 89 173 1.84e-14 74.7
MS.gene030625.t1 MTR_3g096920 38.750 80 45 2 3 79 81 159 4.29e-14 72.8
MS.gene030625.t1 MTR_7g070140 52.727 55 24 1 2 54 37 91 5.00e-14 69.3
MS.gene030625.t1 MTR_5g040420 37.647 85 43 3 3 77 88 172 1.51e-13 72.0
MS.gene030625.t1 MTR_2g068880 36.667 90 42 2 3 77 97 186 4.06e-13 70.1
MS.gene030625.t1 MTR_3g098810 38.947 95 48 2 1 85 84 178 4.92e-13 68.9
MS.gene030625.t1 MTR_2g079990 44.262 61 34 0 3 63 90 150 5.67e-13 70.5
MS.gene030625.t1 MTR_1g097300 53.704 54 25 0 49 102 1 54 6.35e-13 70.5
MS.gene030625.t1 MTR_2g093810 39.506 81 43 3 3 77 88 168 2.65e-12 67.8
MS.gene030625.t1 MTR_4g089135 51.852 54 24 1 3 54 89 142 7.66e-12 66.6
MS.gene030625.t1 MTR_7g070150 43.860 57 29 1 21 77 4 57 9.85e-12 61.2
MS.gene030625.t1 MTR_8g023900 40.506 79 39 4 1 77 268 340 2.26e-11 65.9
MS.gene030625.t1 MTR_7g105170 32.927 82 49 2 2 77 83 164 2.50e-11 63.9
MS.gene030625.t1 MTR_2g068920 50.000 56 24 1 3 54 96 151 2.64e-11 64.3
MS.gene030625.t1 MTR_3g098810 51.724 58 24 1 1 54 84 141 4.14e-11 62.0
MS.gene030625.t1 MTR_2g086690 35.802 81 48 3 3 79 84 164 4.51e-11 63.2
MS.gene030625.t1 MTR_5g090970 33.333 87 46 3 3 77 91 177 4.52e-11 63.9
MS.gene030625.t1 MTR_5g090970 33.333 87 46 3 3 77 92 178 4.63e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030625.t1 AT5G09330 51.899 158 70 2 3 156 82 237 9.42e-45 162
MS.gene030625.t1 AT5G09330 51.899 158 70 2 3 156 82 237 9.42e-45 162
MS.gene030625.t1 AT5G09330 51.899 158 70 2 3 156 82 237 9.42e-45 162
MS.gene030625.t1 AT5G09330 51.899 158 70 2 3 156 82 237 9.42e-45 162
MS.gene030625.t1 AT5G09330 51.899 158 70 2 3 156 82 237 9.42e-45 162
MS.gene030625.t1 AT5G64060 49.660 147 72 1 3 149 82 226 4.22e-42 152
MS.gene030625.t1 AT5G04410 52.174 115 50 1 3 112 85 199 1.22e-38 146
MS.gene030625.t1 AT3G10500 58.889 90 37 0 3 92 85 174 2.58e-35 137
MS.gene030625.t1 AT3G10490 48.739 119 51 3 1 118 101 210 3.11e-32 122
MS.gene030625.t1 AT3G10490 48.739 119 51 3 1 118 1 110 8.18e-32 124
MS.gene030625.t1 AT3G10490 48.739 119 51 3 1 118 1 110 8.18e-32 124
MS.gene030625.t1 AT1G34190 51.515 99 48 0 3 101 92 190 1.18e-31 127
MS.gene030625.t1 AT1G34180 45.872 109 53 1 3 105 92 200 2.15e-31 124
MS.gene030625.t1 AT1G34180 45.872 109 53 1 3 105 104 212 2.75e-31 124
MS.gene030625.t1 AT3G10490 48.739 119 51 3 1 118 101 210 4.89e-31 124
MS.gene030625.t1 AT1G34180 45.872 109 53 1 3 105 92 200 6.04e-31 124
MS.gene030625.t1 AT1G34180 45.872 109 53 1 3 105 104 212 6.09e-31 125
MS.gene030625.t1 AT3G10480 45.238 126 59 3 1 125 101 217 3.96e-30 121
MS.gene030625.t1 AT3G10480 44.800 125 60 2 1 125 101 216 4.32e-30 121
MS.gene030625.t1 AT3G10480 45.238 126 59 3 1 125 134 250 6.84e-30 121
MS.gene030625.t1 AT1G32870 53.261 92 41 1 3 92 84 175 1.12e-27 115
MS.gene030625.t1 AT1G32870 53.261 92 41 1 3 92 84 175 1.27e-27 115
MS.gene030625.t1 AT1G32870 53.261 92 41 1 3 92 120 211 2.07e-27 114
MS.gene030625.t1 AT3G17730 55.844 77 34 0 3 79 82 158 1.82e-25 104
MS.gene030625.t1 AT3G12977 51.852 81 38 1 3 82 39 119 2.16e-24 100
MS.gene030625.t1 AT1G33060 53.333 75 35 0 3 77 100 174 9.80e-24 103
MS.gene030625.t1 AT1G33060 53.333 75 35 0 3 77 100 174 1.01e-23 103
MS.gene030625.t1 AT1G65910 47.674 86 45 0 3 88 82 167 1.69e-23 103
MS.gene030625.t1 AT3G12977 52.500 80 37 1 3 81 92 171 1.74e-23 99.8
MS.gene030625.t1 AT5G61430 49.398 83 40 1 3 83 27 109 7.38e-23 97.8
MS.gene030625.t1 AT4G10350 50.000 76 38 0 3 78 39 114 1.45e-22 97.4
MS.gene030625.t1 AT5G61430 49.398 83 40 1 3 83 90 172 2.70e-22 97.4
MS.gene030625.t1 AT1G71930 35.938 128 69 3 3 126 86 204 2.85e-22 97.1
MS.gene030625.t1 AT1G71930 35.938 128 69 3 3 126 86 204 2.85e-22 97.1
MS.gene030625.t1 AT4G10350 50.000 76 38 0 3 78 86 161 3.47e-22 97.1
MS.gene030625.t1 AT5G39610 48.193 83 41 1 3 83 94 176 2.08e-21 94.0
MS.gene030625.t1 AT1G79580 48.052 77 36 2 3 78 94 167 1.61e-20 92.8
MS.gene030625.t1 AT1G79580 48.052 77 36 2 3 78 94 167 1.61e-20 92.8
MS.gene030625.t1 AT1G79580 48.052 77 36 2 3 78 94 167 1.61e-20 92.8
MS.gene030625.t1 AT1G79580 48.052 77 36 2 3 78 94 167 1.61e-20 92.8
MS.gene030625.t1 AT1G79580 48.052 77 36 2 3 78 94 167 1.61e-20 92.8
MS.gene030625.t1 AT1G33280 47.368 76 37 1 3 78 85 157 1.66e-20 91.7
MS.gene030625.t1 AT1G56010 34.783 184 91 6 3 174 27 193 1.83e-20 90.5
MS.gene030625.t1 AT5G07680 45.783 83 43 1 3 83 77 159 2.19e-20 91.7
MS.gene030625.t1 AT1G12260 40.187 107 58 3 3 108 84 185 2.56e-20 92.4
MS.gene030625.t1 AT4G35580 49.333 75 38 0 3 77 85 159 2.62e-20 93.6
MS.gene030625.t1 AT4G35580 49.333 75 38 0 3 77 85 159 2.66e-20 93.2
MS.gene030625.t1 AT4G35580 49.333 75 38 0 3 77 85 159 2.78e-20 93.2
MS.gene030625.t1 AT5G07680 45.783 83 43 1 3 83 91 173 2.99e-20 91.3
MS.gene030625.t1 AT1G56010 34.783 184 91 6 3 174 94 260 3.06e-20 91.3
MS.gene030625.t1 AT1G12260 40.187 107 58 3 3 108 84 185 3.10e-20 91.7
MS.gene030625.t1 AT3G49530 37.879 132 65 4 3 131 89 206 4.19e-20 92.0
MS.gene030625.t1 AT1G76420 51.899 79 35 2 3 79 96 173 4.33e-20 90.9
MS.gene030625.t1 AT1G62700 39.450 109 58 4 3 108 84 187 4.65e-20 91.7
MS.gene030625.t1 AT1G62700 39.450 109 58 4 3 108 84 187 4.65e-20 91.7
MS.gene030625.t1 AT3G49530 37.879 132 65 4 3 131 89 206 4.71e-20 92.4
MS.gene030625.t1 AT5G24590 46.835 79 41 1 1 78 87 165 6.23e-20 91.7
MS.gene030625.t1 AT2G27300 47.945 73 31 1 2 74 88 153 6.56e-20 90.5
MS.gene030625.t1 AT3G18400 43.210 81 45 1 3 83 79 158 9.00e-20 89.7
MS.gene030625.t1 AT2G18060 46.835 79 40 1 3 81 86 162 3.44e-19 89.0
MS.gene030625.t1 AT2G18060 46.835 79 40 1 3 81 86 162 3.44e-19 89.0
MS.gene030625.t1 AT4G36160 43.750 96 45 2 3 98 87 173 3.60e-19 89.0
MS.gene030625.t1 AT4G36160 43.750 96 45 2 3 98 87 173 3.60e-19 89.0
MS.gene030625.t1 AT4G36160 43.750 96 45 2 3 98 91 177 4.22e-19 88.6
MS.gene030625.t1 AT2G24430 48.052 77 37 2 3 77 90 165 5.05e-19 87.8
MS.gene030625.t1 AT2G24430 48.052 77 37 2 3 77 90 165 5.05e-19 87.8
MS.gene030625.t1 AT2G46770 40.449 89 40 2 3 78 93 181 5.17e-19 88.2
MS.gene030625.t1 AT5G13180 48.810 84 40 2 3 84 85 167 5.37e-19 86.3
MS.gene030625.t1 AT5G18270 48.193 83 36 3 3 80 95 175 5.56e-19 87.8
MS.gene030625.t1 AT5G18270 48.193 83 36 3 3 80 95 175 6.00e-19 87.8
MS.gene030625.t1 AT5G17260 47.368 76 39 1 3 77 82 157 8.80e-19 88.6
MS.gene030625.t1 AT3G29035 42.857 91 50 1 3 91 98 188 1.02e-18 86.7
MS.gene030625.t1 AT3G03200 47.368 76 39 1 3 77 82 157 1.18e-18 88.2
MS.gene030625.t1 AT3G04060 46.914 81 40 2 3 80 94 174 1.38e-18 86.7
MS.gene030625.t1 AT1G69490 36.937 111 64 2 3 109 83 191 1.44e-18 85.5
MS.gene030625.t1 AT2G18060 41.758 91 43 2 8 98 3 83 2.74e-18 84.7
MS.gene030625.t1 AT5G22290 44.595 74 33 2 2 74 95 161 4.96e-18 85.1
MS.gene030625.t1 AT3G61910 40.909 88 39 1 3 77 88 175 5.68e-18 84.7
MS.gene030625.t1 AT5G53950 50.000 80 34 3 3 77 91 169 6.26e-18 85.1
MS.gene030625.t1 AT5G62380 46.053 76 39 1 3 78 84 157 7.01e-18 84.7
MS.gene030625.t1 AT5G62380 46.053 76 39 1 3 78 84 157 7.01e-18 84.7
MS.gene030625.t1 AT3G44290 43.836 73 34 1 2 74 88 153 7.90e-18 82.4
MS.gene030625.t1 AT3G15170 45.349 86 41 3 3 83 94 178 1.77e-17 83.2
MS.gene030625.t1 AT3G44290 43.836 73 34 1 2 74 88 153 2.72e-17 82.8
MS.gene030625.t1 AT1G54330 46.341 82 41 1 3 84 82 160 3.29e-17 82.0
MS.gene030625.t1 AT1G52890 36.170 94 56 1 3 92 88 181 6.52e-17 81.6
MS.gene030625.t1 AT5G66300 33.566 143 90 4 3 143 89 228 7.38e-17 80.9
MS.gene030625.t1 AT2G33480 46.053 76 39 1 3 78 86 159 1.08e-16 80.1
MS.gene030625.t1 AT2G33480 46.053 76 41 0 3 78 29 104 1.17e-16 78.6
MS.gene030625.t1 AT1G32770 38.372 86 43 1 3 78 93 178 1.70e-16 80.9
MS.gene030625.t1 AT3G15500 38.158 76 47 0 3 78 88 163 2.02e-16 80.1
MS.gene030625.t1 AT4G27410 38.667 75 46 0 3 77 88 162 2.60e-16 79.3
MS.gene030625.t1 AT1G01720 36.036 111 62 3 3 105 81 190 2.81e-16 79.3
MS.gene030625.t1 AT2G33480 46.053 76 41 0 3 78 86 161 2.84e-16 79.0
MS.gene030625.t1 AT4G27410 38.667 75 46 0 3 77 105 179 3.01e-16 79.3
MS.gene030625.t1 AT5G22290 43.478 69 31 2 7 74 1 62 3.35e-16 78.2
MS.gene030625.t1 AT5G46590 42.857 77 42 2 3 77 82 158 6.11e-16 78.2
MS.gene030625.t1 AT1G77450 41.250 80 43 2 3 79 84 162 1.20e-15 76.6
MS.gene030625.t1 AT4G28530 46.753 77 35 2 3 77 100 172 1.55e-15 77.8
MS.gene030625.t1 AT5G08790 39.024 82 44 2 3 79 81 161 1.76e-15 76.6
MS.gene030625.t1 AT4G17980 43.038 79 41 2 3 77 82 160 3.01e-15 75.9
MS.gene030625.t1 AT4G17980 43.038 79 41 2 3 77 82 160 3.40e-15 76.3
MS.gene030625.t1 AT5G63790 39.024 82 44 2 3 79 134 214 4.38e-15 76.3
MS.gene030625.t1 AT5G63790 39.024 82 44 2 3 79 124 204 4.56e-15 75.9
MS.gene030625.t1 AT3G15510 42.045 88 38 4 3 77 91 178 1.52e-14 75.1
MS.gene030625.t1 AT1G61110 38.636 88 41 2 3 77 90 177 1.96e-14 74.3
MS.gene030625.t1 AT1G32510 38.824 85 44 2 1 77 80 164 2.04e-14 73.6
MS.gene030625.t1 AT5G46590 49.153 59 29 1 3 60 82 140 2.91e-14 73.2
MS.gene030625.t1 AT2G02450 39.744 78 44 2 3 79 125 200 5.21e-14 73.6
MS.gene030625.t1 AT2G02450 39.744 78 44 2 3 79 125 200 7.44e-14 73.2
MS.gene030625.t1 AT2G43000 39.474 76 45 1 3 77 14 89 1.09e-13 70.1
MS.gene030625.t1 AT2G17040 41.026 78 38 2 3 77 80 152 1.61e-13 70.9
MS.gene030625.t1 AT3G04070 38.636 88 40 3 3 77 84 170 1.78e-13 71.6
MS.gene030625.t1 AT4G01540 45.000 80 36 2 1 77 80 154 2.01e-13 72.0
MS.gene030625.t1 AT1G26870 38.372 86 42 3 3 77 90 175 2.16e-13 72.0
MS.gene030625.t1 AT4G01540 45.000 80 36 2 1 77 80 154 2.28e-13 71.6
MS.gene030625.t1 AT4G01520 43.750 80 37 2 1 77 80 154 3.11e-13 70.5
MS.gene030625.t1 AT4G01540 45.000 80 36 2 1 77 80 154 3.52e-13 71.2
MS.gene030625.t1 AT4G01540 45.000 80 36 2 1 77 80 154 3.64e-13 71.2
MS.gene030625.t1 AT2G43000 39.474 76 45 1 3 77 92 167 5.92e-13 69.3
MS.gene030625.t1 AT5G04395 50.820 61 27 1 1 58 113 173 6.97e-13 67.4
MS.gene030625.t1 AT3G04070 30.097 103 44 2 3 77 84 186 5.44e-12 67.0
MS.gene030625.t1 AT4G01550 40.964 83 33 3 2 77 80 153 9.02e-12 67.0
MS.gene030625.t1 AT1G52880 39.080 87 41 3 3 77 91 177 8.53e-11 63.2

Find 117 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTTCGACTCCAACTTTC+AGG 0.173690 4.3:+8218844 None:intergenic
ATGAAACGAGCTACGGAAAT+TGG 0.193778 4.3:-8220917 MS.gene030625:CDS
CGTGGATTGCTCGTCTGATT+TGG 0.251020 4.3:-8218995 MS.gene030625:CDS
TGGCTTCTACGGGCAATGTT+GGG 0.251527 4.3:-8218933 MS.gene030625:CDS
CCTAGGAATGGTGCACAATA+TGG 0.269913 4.3:-8220035 MS.gene030625:CDS
CCTGTTTGACATGGATTTGC+AGG 0.278572 4.3:+8219780 None:intergenic
CACACACGGGCTGGCTTCTA+CGG 0.300862 4.3:-8218944 MS.gene030625:CDS
CTCCAAACTAGTGGATATAT+TGG 0.305281 4.3:-8219891 MS.gene030625:CDS
TCATTAACAGTTTGATTGTC+TGG 0.311175 4.3:+8219354 None:intergenic
CTCCAGTCTTCCTCATTAAA+TGG 0.323348 4.3:+8220008 None:intergenic
ATTAGATGTCACAGGCAAAC+TGG 0.327321 4.3:+8219938 None:intergenic
CTGGCTTCTACGGGCAATGT+TGG 0.327912 4.3:-8218934 MS.gene030625:CDS
GGCTTCTACGGGCAATGTTG+GGG 0.330102 4.3:-8218932 MS.gene030625:CDS
AACCAATATATCCACTAGTT+TGG 0.339769 4.3:+8219889 None:intergenic
CTTGCGAGTTCATGTCAAAT+AGG 0.339836 4.3:-8219525 MS.gene030625:CDS
GTGGATTGCTCGTCTGATTT+GGG 0.344683 4.3:-8218994 MS.gene030625:CDS
GAGCTACGGAAATTGGGTTT+TGG 0.345895 4.3:-8220910 MS.gene030625:CDS
ATCAGAATTGGCGCCTTCTC+TGG 0.347156 4.3:-8219847 MS.gene030625:CDS
CCTTCAAATTCTTGTCAAAC+AGG 0.351866 4.3:-8219651 MS.gene030625:CDS
TCTGTTTGACATGAATTTGC+AGG 0.364697 4.3:+8219567 None:intergenic
CCTGTTTGACATGAATTTGC+TGG 0.368512 4.3:+8219693 None:intergenic
TGACAAGAATTTGAAGGGTC+TGG 0.381024 4.3:+8219657 None:intergenic
GCATGACGACTTCGATTTCA+TGG 0.381469 4.3:-8218908 MS.gene030625:CDS
TCTGGATATGAATCATTTGC+AGG 0.389690 4.3:+8219372 None:intergenic
TCTGGATATGAATCATTTGC+AGG 0.389690 4.3:+8219483 None:intergenic
TCGTTAACAGCTTGATTGTC+TGG 0.392462 4.3:+8219465 None:intergenic
TGACAAGAATTCACAGGGTC+TGG 0.397096 4.3:+8219615 None:intergenic
CCTGTGAATTCTTGTCAAAC+AGG 0.404297 4.3:-8219609 MS.gene030625:CDS
TGACATGGATTTGCAGGGTC+AGG 0.408876 4.3:+8219786 None:intergenic
CCTGTGAATCCTTGTCAAAC+AGG 0.414356 4.3:-8219738 MS.gene030625:CDS
TCGACTCCAACTTTCAGGTT+GGG 0.415732 4.3:+8218849 None:intergenic
CCTGTTTGACAAGAATTTGA+AGG 0.420779 4.3:+8219651 None:intergenic
TGACAAGGATTCACAGGGTC+TGG 0.422865 4.3:+8219744 None:intergenic
CTGTTTGACATGAATTTGTT+GGG 0.425559 4.3:+8219823 None:intergenic
TGACATGAACTCGCAAGGTC+AGG 0.427713 4.3:+8219531 None:intergenic
CCAACAAATTCATGTCAAAC+AGG 0.430400 4.3:-8219822 MS.gene030625:CDS
CCTGTTTGACATGAATTTGT+TGG 0.430797 4.3:+8219822 None:intergenic
TGAAACGAGCTACGGAAATT+GGG 0.444296 4.3:-8220916 MS.gene030625:CDS
CCTGCAAATCCATGTCAAAC+AGG 0.444856 4.3:-8219780 MS.gene030625:CDS
GGCTGACAAAGGAATTGTTC+AGG 0.445801 4.3:-8220741 MS.gene030625:intron
CCATATTGTGCACCATTCCT+AGG 0.447789 4.3:+8220035 None:intergenic
GTGTGCTAAAGTCTGCATTC+TGG 0.450394 4.3:+8218962 None:intergenic
CCAGCAAATTCATGTCAAAC+AGG 0.451371 4.3:-8219693 MS.gene030625:CDS
ATCTAGACTCTGATACGTTC+TGG 0.454030 4.3:-8218876 MS.gene030625:CDS
TGACATGAATTTGCTGGGTC+AGG 0.454766 4.3:+8219699 None:intergenic
CATACCGTGTCAATCAGAAT+TGG 0.460006 4.3:-8219859 MS.gene030625:CDS
TGAGTATAGGCTTGAAGATA+AGG 0.460176 4.3:-8220768 MS.gene030625:CDS
GAATTCACAGGGTCTGGATA+AGG 0.466095 4.3:+8219621 None:intergenic
GGATTCACAGGGTCTGGATA+AGG 0.468391 4.3:+8219750 None:intergenic
TATGAATCATTTGCAGGGTC+GGG 0.476385 4.3:+8219378 None:intergenic
CTAGGAATGGTGCACAATAT+GGG 0.477134 4.3:-8220034 MS.gene030625:CDS
TATGAATCATTTGCAGGGTC+GGG 0.478405 4.3:+8219489 None:intergenic
TCATTTGCAGGGTCGGGCGC+AGG 0.479506 4.3:+8219384 None:intergenic
ATTGGGTTATGCATGAGTAT+AGG 0.479985 4.3:-8220781 MS.gene030625:CDS
GAATTTGCAGGGTCGGAAGC+AGG 0.480953 4.3:+8219579 None:intergenic
TTCGACTCCAACTTTCAGGT+TGG 0.483370 4.3:+8218848 None:intergenic
CCTGTTTGACAAGGATTCAC+AGG 0.487590 4.3:+8219738 None:intergenic
GACCATTTAATGAGGAAGAC+TGG 0.495616 4.3:-8220010 MS.gene030625:CDS
TCGTGGTGATCGAACCGATT+GGG 0.500477 4.3:-8220798 MS.gene030625:CDS
GGCAGACCCAACCTGAAAGT+TGG 0.501795 4.3:-8218855 MS.gene030625:CDS
CCTGTTTGACAAGAATTCAC+AGG 0.507170 4.3:+8219609 None:intergenic
ACACACGGGCTGGCTTCTAC+GGG 0.510540 4.3:-8218943 MS.gene030625:CDS
AGTGCAGCAGATTCAGCAAA+AGG 0.512913 4.3:+8219966 None:intergenic
GCTTCTACGGGCAATGTTGG+GGG 0.513160 4.3:-8218931 MS.gene030625:CDS
AGACTTTAGCACACACGGGC+TGG 0.515131 4.3:-8218953 MS.gene030625:CDS
TCGTCTGATTTGGGATCTGT+TGG 0.516876 4.3:-8218985 MS.gene030625:CDS
CTGTTTGACATGAATTTGCT+GGG 0.522565 4.3:+8219694 None:intergenic
CAATTCTGATTGACACGGTA+TGG 0.524497 4.3:+8219860 None:intergenic
CTGTTTGACATGAATTTGCA+GGG 0.530109 4.3:+8219568 None:intergenic
CTCGTGGTGATCGAACCGAT+TGG 0.531457 4.3:-8220799 MS.gene030625:CDS
ATATGAATCATTTGCAGGGT+CGG 0.532792 4.3:+8219377 None:intergenic
GAATTTGTTGGGTCCAGAGA+AGG 0.534027 4.3:+8219834 None:intergenic
ATATGAATCATTTGCAGGGT+CGG 0.534812 4.3:+8219488 None:intergenic
GGATTCCTACGTAATCTGTA+AGG 0.535028 4.3:-8220081 MS.gene030625:CDS
CTGTTTGACAAGAATTTGAA+GGG 0.535613 4.3:+8219652 None:intergenic
TCATTTGCAGGGTCGGGCAA+AGG 0.536379 4.3:+8219495 None:intergenic
GACATGAACTCGCAAGGTCA+GGG 0.545876 4.3:+8219532 None:intergenic
CTGTTTGACATGGATTTGCA+GGG 0.546270 4.3:+8219781 None:intergenic
GATCAGAATCTCCAAACTAG+TGG 0.547413 4.3:-8219900 MS.gene030625:CDS
TAGGCTTGAAGATAAGGACT+TGG 0.547580 4.3:-8220762 MS.gene030625:CDS
CAACAGAATAATCAAACGGT+GGG 0.550179 4.3:-8220860 MS.gene030625:CDS
ATACTCATGCATAACCCAAT+CGG 0.550912 4.3:+8220784 None:intergenic
GAATTTGAAGGGTCTGGAGA+AGG 0.554806 4.3:+8219663 None:intergenic
TTGACATGAATTTGCAGGGT+CGG 0.556895 4.3:+8219572 None:intergenic
GAATTTGCTGGGTCAGGAGA+CGG 0.560757 4.3:+8219705 None:intergenic
GGCATAAATCCTGTTTGACA+TGG 0.562278 4.3:+8219771 None:intergenic
GACTGGAGTGATGATGAAGT+AGG 0.564465 4.3:-8219993 MS.gene030625:CDS
CTTCTACGGGCAATGTTGGG+GGG 0.567691 4.3:-8218930 MS.gene030625:CDS
GGATTTGCAGGGTCAGGAGA+AGG 0.569179 4.3:+8219792 None:intergenic
TTAGATGTCACAGGCAAACT+GGG 0.572296 4.3:+8219939 None:intergenic
GATAAGGACTTGGCTGACAA+AGG 0.573980 4.3:-8220752 MS.gene030625:CDS
GAAGGTCCTGGTCCTAGGAA+TGG 0.591114 4.3:-8220047 MS.gene030625:CDS
CTGTTTGACAAGAATTCACA+GGG 0.591836 4.3:+8219610 None:intergenic
CTGTTTGACAAGGATTCACA+GGG 0.595085 4.3:+8219739 None:intergenic
TGTTCAACAGAATAATCAAA+CGG 0.610587 4.3:-8220864 MS.gene030625:CDS
CTATTTGACATGAACTCGCA+AGG 0.614626 4.3:+8219526 None:intergenic
ACAGAACTATTAGATGTCAC+AGG 0.615324 4.3:+8219930 None:intergenic
AGACAACGGCGTATTGCCTG+AGG 0.618252 4.3:-8219295 MS.gene030625:intron
CTGGATATGAATCATTTGCA+GGG 0.618285 4.3:+8219373 None:intergenic
CTGGATATGAATCATTTGCA+GGG 0.618285 4.3:+8219484 None:intergenic
AATGCAGACTTTAGCACACA+CGG 0.626353 4.3:-8218958 MS.gene030625:CDS
ATAAATCCTCCTGTTTGACA+AGG 0.629631 4.3:+8219729 None:intergenic
AAAACCCTAATATTTCACAC+TGG 0.629779 4.3:-8220830 MS.gene030625:CDS
GCCAAGGATGAAACGAGCTA+CGG 0.632310 4.3:-8220924 None:intergenic
TGTGCACCATTCCTAGGACC+AGG 0.638891 4.3:+8220041 None:intergenic
ACATGAACTCGCAAGGTCAG+GGG 0.640717 4.3:+8219533 None:intergenic
ATATGGGAGACCATTTAATG+AGG 0.644918 4.3:-8220018 MS.gene030625:CDS
GTGAATCCTTGTCAAACAGG+AGG 0.649506 4.3:-8219735 MS.gene030625:CDS
AACAGAATAATCAAACGGTG+GGG 0.675165 4.3:-8220859 MS.gene030625:CDS
AAATACCTTACAGATTACGT+AGG 0.675415 4.3:+8220076 None:intergenic
GAACTCGCAAGGTCAGGGGA+CGG 0.679790 4.3:+8219537 None:intergenic
TCAACAGAATAATCAAACGG+TGG 0.680625 4.3:-8220861 MS.gene030625:CDS
ATGCAGACTTTAGCACACAC+GGG 0.691963 4.3:-8218957 MS.gene030625:CDS
TGTCAGGAAAACATTGCTGA+AGG 0.693858 4.3:-8219054 MS.gene030625:intron
GGCGCCAATTCTGATTGACA+CGG 0.715605 4.3:+8219855 None:intergenic
CACACTGGAAAATCACCTCG+TGG 0.720810 4.3:-8220815 MS.gene030625:CDS
CAATCGGTTCGATCACCACG+AGG 0.739524 4.3:+8220800 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTAAAAGAAAGCAA+GGG + chr4.3:8219593-8219612 None:intergenic 15.0%
!! AATAACATAATTCTATTTCA+AGG + chr4.3:8219427-8219446 None:intergenic 15.0%
!! AGTAAAAAATGCACTTTATA+AGG + chr4.3:8219469-8219488 None:intergenic 20.0%
!! CAAATAATTAAAAGAAAGCA+AGG + chr4.3:8219594-8219613 None:intergenic 20.0%
! AAAATGGCATTAACATTATG+AGG + chr4.3:8220614-8220633 None:intergenic 25.0%
! ATATGGAGATATATGTGTAT+TGG - chr4.3:8219075-8219094 MS.gene030625:intron 25.0%
! ATCGCTATGATTTAGTAAAA+TGG + chr4.3:8220630-8220649 None:intergenic 25.0%
! TGATGTTTCTTTATTATGTC+TGG - chr4.3:8220537-8220556 MS.gene030625:intron 25.0%
! TGTTCAACAGAATAATCAAA+CGG - chr4.3:8218893-8218912 MS.gene030625:CDS 25.0%
!! ATTTTCCAGTGTGAAATATT+AGG + chr4.3:8218935-8218954 None:intergenic 25.0%
!! TGTAAGGTATTTCAAAAAGA+AGG - chr4.3:8219692-8219711 MS.gene030625:CDS 25.0%
!! TTTTCCAGTGTGAAATATTA+GGG + chr4.3:8218934-8218953 None:intergenic 25.0%
!!! ATGTTTTTAACAAGTTCTCT+TGG - chr4.3:8219646-8219665 MS.gene030625:CDS 25.0%
!!! TGTTTTGTATGAGCTTTATA+TGG - chr4.3:8219058-8219077 MS.gene030625:intron 25.0%
AAAACCCTAATATTTCACAC+TGG - chr4.3:8218927-8218946 MS.gene030625:CDS 30.0%
AAAGTAGTTGTGTCCTTTAA+TGG + chr4.3:8219301-8219320 None:intergenic 30.0%
AAATACCTTACAGATTACGT+AGG + chr4.3:8219684-8219703 None:intergenic 30.0%
AACATATTCACAACGAGAAT+AGG + chr4.3:8219631-8219650 None:intergenic 30.0%
AACCAATATATCCACTAGTT+TGG + chr4.3:8219871-8219890 None:intergenic 30.0%
ACACAACTACTTTAAATGCT+AGG - chr4.3:8219308-8219327 MS.gene030625:CDS 30.0%
AGTTATCCTTCAAGAAATCA+AGG + chr4.3:8220662-8220681 None:intergenic 30.0%
CACAACTACTTTAAATGCTA+GGG - chr4.3:8219309-8219328 MS.gene030625:CDS 30.0%
CTGTTTGACAAGAATTTGAA+GGG + chr4.3:8220108-8220127 None:intergenic 30.0%
TACTTTAAATGCTAGGGTTA+GGG - chr4.3:8219315-8219334 MS.gene030625:CDS 30.0%
TATAGTCCTTGATTTCTTGA+AGG - chr4.3:8220653-8220672 MS.gene030625:intron 30.0%
TCATTAACAGTTTGATTGTC+TGG + chr4.3:8220406-8220425 None:intergenic 30.0%
TCTTATCTGTTACATTGGAA+AGG + chr4.3:8219211-8219230 None:intergenic 30.0%
! CTGTTTGACATGAATTTGTT+GGG + chr4.3:8219937-8219956 None:intergenic 30.0%
! CTTGATATTTTCGAAGACAA+CGG - chr4.3:8220448-8220467 MS.gene030625:intron 30.0%
! TCTTTCTACTTGTGTTTTTC+TGG - chr4.3:8220506-8220525 MS.gene030625:intron 30.0%
! TTGACATTATTATTGGTGTC+AGG - chr4.3:8220687-8220706 MS.gene030625:intron 30.0%
!! CTGAGTGTTGACATTATTAT+TGG - chr4.3:8220680-8220699 MS.gene030625:intron 30.0%
!!! CTCTTAACTGACTTTTTTGA+TGG - chr4.3:8220730-8220749 MS.gene030625:intron 30.0%
AAAACAGATTAGCATACCTC+AGG + chr4.3:8220481-8220500 None:intergenic 35.0%
AACAGAATAATCAAACGGTG+GGG - chr4.3:8218898-8218917 MS.gene030625:CDS 35.0%
AATGGCATTAGTGACACAAT+AGG + chr4.3:8219283-8219302 None:intergenic 35.0%
ACAGAACTATTAGATGTCAC+AGG + chr4.3:8219830-8219849 None:intergenic 35.0%
ATAAATCCTCCTGTTTGACA+AGG + chr4.3:8220031-8220050 None:intergenic 35.0%
ATACTCATGCATAACCCAAT+CGG + chr4.3:8218976-8218995 None:intergenic 35.0%
ATATGAATCATTTGCAGGGT+CGG + chr4.3:8220272-8220291 None:intergenic 35.0%
ATATGGGAGACCATTTAATG+AGG - chr4.3:8219739-8219758 MS.gene030625:CDS 35.0%
ATATTGAACTGGTGAATACG+TGG - chr4.3:8219105-8219124 MS.gene030625:intron 35.0%
ATTGGGTTATGCATGAGTAT+AGG - chr4.3:8218976-8218995 MS.gene030625:CDS 35.0%
CAACAGAATAATCAAACGGT+GGG - chr4.3:8218897-8218916 MS.gene030625:CDS 35.0%
CCAACAAATTCATGTCAAAC+AGG - chr4.3:8219935-8219954 MS.gene030625:CDS 35.0%
CCTGTTTGACAAGAATTTGA+AGG + chr4.3:8220109-8220128 None:intergenic 35.0%
CCTGTTTGACATGAATTTGT+TGG + chr4.3:8219938-8219957 None:intergenic 35.0%
CCTTCAAATTCTTGTCAAAC+AGG - chr4.3:8220106-8220125 MS.gene030625:intron 35.0%
CTACTTTAAATGCTAGGGTT+AGG - chr4.3:8219314-8219333 MS.gene030625:CDS 35.0%
CTGGATATGAATCATTTGCA+GGG + chr4.3:8220276-8220295 None:intergenic 35.0%
CTGTTTGACAAGAATTCACA+GGG + chr4.3:8220150-8220169 None:intergenic 35.0%
CTGTTTGACATGAATTTGCA+GGG + chr4.3:8220192-8220211 None:intergenic 35.0%
GTATTCACCAGTTCAATATG+TGG + chr4.3:8219104-8219123 None:intergenic 35.0%
GTATTTCAAAAAGAAGGTCC+TGG - chr4.3:8219698-8219717 MS.gene030625:CDS 35.0%
TATTCACAACGAGAATAGGA+TGG + chr4.3:8219627-8219646 None:intergenic 35.0%
TCAACAGAATAATCAAACGG+TGG - chr4.3:8218896-8218915 MS.gene030625:CDS 35.0%
TCCAATGTAACAGATAAGAC+CGG - chr4.3:8219212-8219231 MS.gene030625:intron 35.0%
TCTGGATATGAATCATTTGC+AGG + chr4.3:8220277-8220296 None:intergenic 35.0%
TCTGTTTGACATGAATTTGC+AGG + chr4.3:8220193-8220212 None:intergenic 35.0%
TGAGTATAGGCTTGAAGATA+AGG - chr4.3:8218989-8219008 MS.gene030625:CDS 35.0%
TGGAAAGGAATATATGCCAA+TGG + chr4.3:8219196-8219215 None:intergenic 35.0%
TTATATGCATTCGCAGTCAA+AGG + chr4.3:8219147-8219166 None:intergenic 35.0%
TTATTTCGACTCCAACTTTC+AGG + chr4.3:8220916-8220935 None:intergenic 35.0%
! CTCCAAACTAGTGGATATAT+TGG - chr4.3:8219866-8219885 MS.gene030625:CDS 35.0%
! CTGTTTGACATGAATTTGCT+GGG + chr4.3:8220066-8220085 None:intergenic 35.0%
! TGTGCATCAAAAGCATATTC+CGG + chr4.3:8219234-8219253 None:intergenic 35.0%
! TGTGTCACTAATGCCATTAA+AGG - chr4.3:8219285-8219304 MS.gene030625:intron 35.0%
!! TTCATGATAGTTTTGCATGC+AGG - chr4.3:8219396-8219415 MS.gene030625:CDS 35.0%
!!! AACTGACTTTTTTGATGGCT+TGG - chr4.3:8220735-8220754 MS.gene030625:intron 35.0%
AATGCAGACTTTAGCACACA+CGG - chr4.3:8220799-8220818 MS.gene030625:CDS 40.0%
ACAAGTTCTCTTGGTATGAC+AGG - chr4.3:8219655-8219674 MS.gene030625:CDS 40.0%
ATCTAGACTCTGATACGTTC+TGG - chr4.3:8220881-8220900 MS.gene030625:CDS 40.0%
ATGAAACGAGCTACGGAAAT+TGG - chr4.3:8218840-8218859 MS.gene030625:CDS 40.0%
ATTAGATGTCACAGGCAAAC+TGG + chr4.3:8219822-8219841 None:intergenic 40.0%
CAATTCTGATTGACACGGTA+TGG + chr4.3:8219900-8219919 None:intergenic 40.0%
CATACCGTGTCAATCAGAAT+TGG - chr4.3:8219898-8219917 MS.gene030625:CDS 40.0%
CCAGCAAATTCATGTCAAAC+AGG - chr4.3:8220064-8220083 MS.gene030625:CDS 40.0%
CCTGTGAATTCTTGTCAAAC+AGG - chr4.3:8220148-8220167 MS.gene030625:intron 40.0%
CCTGTTTGACAAGAATTCAC+AGG + chr4.3:8220151-8220170 None:intergenic 40.0%
CCTGTTTGACATGAATTTGC+TGG + chr4.3:8220067-8220086 None:intergenic 40.0%
CTAGGAATGGTGCACAATAT+GGG - chr4.3:8219723-8219742 MS.gene030625:CDS 40.0%
CTATTTGACATGAACTCGCA+AGG + chr4.3:8220234-8220253 None:intergenic 40.0%
CTCCAGTCTTCCTCATTAAA+TGG + chr4.3:8219752-8219771 None:intergenic 40.0%
CTGTTTGACAAGGATTCACA+GGG + chr4.3:8220021-8220040 None:intergenic 40.0%
CTGTTTGACATGGATTTGCA+GGG + chr4.3:8219979-8219998 None:intergenic 40.0%
CTTGCGAGTTCATGTCAAAT+AGG - chr4.3:8220232-8220251 MS.gene030625:intron 40.0%
GACCATTTAATGAGGAAGAC+TGG - chr4.3:8219747-8219766 MS.gene030625:CDS 40.0%
GATCAGAATCTCCAAACTAG+TGG - chr4.3:8219857-8219876 MS.gene030625:CDS 40.0%
GGATTCCTACGTAATCTGTA+AGG - chr4.3:8219676-8219695 MS.gene030625:CDS 40.0%
GGCATAAATCCTGTTTGACA+TGG + chr4.3:8219989-8220008 None:intergenic 40.0%
GTATCAAGACTGCGAGTAAA+AGG + chr4.3:8219174-8219193 None:intergenic 40.0%
TAGGCTTGAAGATAAGGACT+TGG - chr4.3:8218995-8219014 MS.gene030625:CDS 40.0%
TATGAATCATTTGCAGGGTC+GGG + chr4.3:8220271-8220290 None:intergenic 40.0%
TCGTTAACAGCTTGATTGTC+TGG + chr4.3:8220295-8220314 None:intergenic 40.0%
TGAAACGAGCTACGGAAATT+GGG - chr4.3:8218841-8218860 MS.gene030625:CDS 40.0%
TGACAAGAATTTGAAGGGTC+TGG + chr4.3:8220103-8220122 None:intergenic 40.0%
TGTCAGGAAAACATTGCTGA+AGG - chr4.3:8220703-8220722 MS.gene030625:intron 40.0%
TTAGATGTCACAGGCAAACT+GGG + chr4.3:8219821-8219840 None:intergenic 40.0%
TTGACATGAATTTGCAGGGT+CGG + chr4.3:8220188-8220207 None:intergenic 40.0%
TTGGTTGCCACATATTGAAC+TGG - chr4.3:8219094-8219113 MS.gene030625:intron 40.0%
! TCCGGTCTTATCTGTTACAT+TGG + chr4.3:8219216-8219235 None:intergenic 40.0%
!!! ATTGGGTTTTGGAAAGCTAC+TGG - chr4.3:8218858-8218877 MS.gene030625:CDS 40.0%
AAAAAGAAGGTCCTGGTCCT+AGG - chr4.3:8219705-8219724 MS.gene030625:CDS 45.0%
ACTCGCAGTCTTGATACCAT+TGG - chr4.3:8219177-8219196 MS.gene030625:intron 45.0%
AGGAGACAATGCAAACTGCA+GGG + chr4.3:8220594-8220613 None:intergenic 45.0%
AGTGCAGCAGATTCAGCAAA+AGG + chr4.3:8219794-8219813 None:intergenic 45.0%
ATCCTTCGAAGGGTGAAATG+TGG - chr4.3:8219340-8219359 MS.gene030625:CDS 45.0%
ATGCAGACTTTAGCACACAC+GGG - chr4.3:8220800-8220819 MS.gene030625:CDS 45.0%
CCATATTGTGCACCATTCCT+AGG + chr4.3:8219725-8219744 None:intergenic 45.0%
CCTAGGAATGGTGCACAATA+TGG - chr4.3:8219722-8219741 MS.gene030625:CDS 45.0%
CCTGCAAATCCATGTCAAAC+AGG - chr4.3:8219977-8219996 MS.gene030625:CDS 45.0%
CCTGTGAATCCTTGTCAAAC+AGG - chr4.3:8220019-8220038 MS.gene030625:CDS 45.0%
CCTGTTTGACAAGGATTCAC+AGG + chr4.3:8220022-8220041 None:intergenic 45.0%
CCTGTTTGACATGGATTTGC+AGG + chr4.3:8219980-8219999 None:intergenic 45.0%
GAATTCACAGGGTCTGGATA+AGG + chr4.3:8220139-8220158 None:intergenic 45.0%
GAATTTGAAGGGTCTGGAGA+AGG + chr4.3:8220097-8220116 None:intergenic 45.0%
GATAAGGACTTGGCTGACAA+AGG - chr4.3:8219005-8219024 MS.gene030625:CDS 45.0%
GCATGACGACTTCGATTTCA+TGG - chr4.3:8220849-8220868 MS.gene030625:CDS 45.0%
GGCATTAGTGACACAATAGG+TGG + chr4.3:8219280-8219299 None:intergenic 45.0%
GGCTGACAAAGGAATTGTTC+AGG - chr4.3:8219016-8219035 MS.gene030625:CDS 45.0%
GGTTAGGGTTATCCTTCGAA+GGG - chr4.3:8219330-8219349 MS.gene030625:CDS 45.0%
GTGAATCCTTGTCAAACAGG+AGG - chr4.3:8220022-8220041 MS.gene030625:CDS 45.0%
GTGTGCTAAAGTCTGCATTC+TGG + chr4.3:8220798-8220817 None:intergenic 45.0%
TGACAAGAATTCACAGGGTC+TGG + chr4.3:8220145-8220164 None:intergenic 45.0%
! GACTGGAGTGATGATGAAGT+AGG - chr4.3:8219764-8219783 MS.gene030625:CDS 45.0%
! GAGCTACGGAAATTGGGTTT+TGG - chr4.3:8218847-8218866 MS.gene030625:CDS 45.0%
! GTGGATTGCTCGTCTGATTT+GGG - chr4.3:8220763-8220782 MS.gene030625:CDS 45.0%
! TTCGACTCCAACTTTCAGGT+TGG + chr4.3:8220912-8220931 None:intergenic 45.0%
!! GAATTTGTTGGGTCCAGAGA+AGG + chr4.3:8219926-8219945 None:intergenic 45.0%
!! TCGACTCCAACTTTCAGGTT+GGG + chr4.3:8220911-8220930 None:intergenic 45.0%
!! TCGTCTGATTTGGGATCTGT+TGG - chr4.3:8220772-8220791 MS.gene030625:CDS 45.0%
!! TGACATGAATTTGCTGGGTC+AGG + chr4.3:8220061-8220080 None:intergenic 45.0%
!! TTTTGATGGCTTGGAAGACG+TGG - chr4.3:8220744-8220763 MS.gene030625:CDS 45.0%
!!! GTTTTGGAAAGCTACTGGTC+AGG - chr4.3:8218863-8218882 MS.gene030625:CDS 45.0%
ACATGAACTCGCAAGGTCAG+GGG + chr4.3:8220227-8220246 None:intergenic 50.0%
ATCAGAATTGGCGCCTTCTC+TGG - chr4.3:8219910-8219929 MS.gene030625:CDS 50.0%
CACACTGGAAAATCACCTCG+TGG - chr4.3:8218942-8218961 MS.gene030625:CDS 50.0%
CTCCACATTTCACCCTTCGA+AGG + chr4.3:8219345-8219364 None:intergenic 50.0%
GACATGAACTCGCAAGGTCA+GGG + chr4.3:8220228-8220247 None:intergenic 50.0%
GAGGAGACAATGCAAACTGC+AGG + chr4.3:8220595-8220614 None:intergenic 50.0%
GGATTCACAGGGTCTGGATA+AGG + chr4.3:8220010-8220029 None:intergenic 50.0%
GGCGCCAATTCTGATTGACA+CGG + chr4.3:8219905-8219924 None:intergenic 50.0%
GGGTTAGGGTTATCCTTCGA+AGG - chr4.3:8219329-8219348 MS.gene030625:CDS 50.0%
TCGTGGTGATCGAACCGATT+GGG - chr4.3:8218959-8218978 MS.gene030625:CDS 50.0%
TGACAAGGATTCACAGGGTC+TGG + chr4.3:8220016-8220035 None:intergenic 50.0%
TGACATGAACTCGCAAGGTC+AGG + chr4.3:8220229-8220248 None:intergenic 50.0%
TGACATGGATTTGCAGGGTC+AGG + chr4.3:8219974-8219993 None:intergenic 50.0%
TGGCTTCTACGGGCAATGTT+GGG - chr4.3:8220824-8220843 MS.gene030625:CDS 50.0%
! CGTGGATTGCTCGTCTGATT+TGG - chr4.3:8220762-8220781 MS.gene030625:CDS 50.0%
!! GAATTTGCTGGGTCAGGAGA+CGG + chr4.3:8220055-8220074 None:intergenic 50.0%
AGACAACGGCGTATTGCCTG+AGG - chr4.3:8220462-8220481 MS.gene030625:intron 55.0%
AGACTTTAGCACACACGGGC+TGG - chr4.3:8220804-8220823 MS.gene030625:CDS 55.0%
CTCGTGGTGATCGAACCGAT+TGG - chr4.3:8218958-8218977 MS.gene030625:CDS 55.0%
CTGGCTTCTACGGGCAATGT+TGG - chr4.3:8220823-8220842 MS.gene030625:CDS 55.0%
CTTCTACGGGCAATGTTGGG+GGG - chr4.3:8220827-8220846 MS.gene030625:CDS 55.0%
GAAGGTCCTGGTCCTAGGAA+TGG - chr4.3:8219710-8219729 MS.gene030625:CDS 55.0%
GAATTTGCAGGGTCGGAAGC+AGG + chr4.3:8220181-8220200 None:intergenic 55.0%
GCTTCTACGGGCAATGTTGG+GGG - chr4.3:8220826-8220845 MS.gene030625:CDS 55.0%
GGATTTGCAGGGTCAGGAGA+AGG + chr4.3:8219968-8219987 None:intergenic 55.0%
GGCAGACCCAACCTGAAAGT+TGG - chr4.3:8220902-8220921 MS.gene030625:CDS 55.0%
GGCTTCTACGGGCAATGTTG+GGG - chr4.3:8220825-8220844 MS.gene030625:CDS 55.0%
TCATTTGCAGGGTCGGGCAA+AGG + chr4.3:8220265-8220284 None:intergenic 55.0%
TGTGCACCATTCCTAGGACC+AGG + chr4.3:8219719-8219738 None:intergenic 55.0%
!! CAATCGGTTCGATCACCACG+AGG + chr4.3:8218960-8218979 None:intergenic 55.0%
GAACTCGCAAGGTCAGGGGA+CGG + chr4.3:8220223-8220242 None:intergenic 60.0%
!! ACACACGGGCTGGCTTCTAC+GGG - chr4.3:8220814-8220833 MS.gene030625:CDS 60.0%
!! CACACACGGGCTGGCTTCTA+CGG - chr4.3:8220813-8220832 MS.gene030625:CDS 60.0%
TCATTTGCAGGGTCGGGCGC+AGG + chr4.3:8220376-8220395 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 8218840 8220939 8218840 ID=MS.gene030625
chr4.3 mRNA 8218840 8220939 8218840 ID=MS.gene030625.t1;Parent=MS.gene030625
chr4.3 exon 8220742 8220939 8220742 ID=MS.gene030625.t1.exon1;Parent=MS.gene030625.t1
chr4.3 CDS 8220742 8220939 8220742 ID=cds.MS.gene030625.t1;Parent=MS.gene030625.t1
chr4.3 exon 8219296 8220102 8219296 ID=MS.gene030625.t1.exon2;Parent=MS.gene030625.t1
chr4.3 CDS 8219296 8220102 8219296 ID=cds.MS.gene030625.t1;Parent=MS.gene030625.t1
chr4.3 exon 8218840 8219070 8218840 ID=MS.gene030625.t1.exon3;Parent=MS.gene030625.t1
chr4.3 CDS 8218840 8219070 8218840 ID=cds.MS.gene030625.t1;Parent=MS.gene030625.t1
Gene Sequence

>MS.gene030625

ATGAAACGAGCTACGGAAATTGGGTTTTGGAAAGCTACTGGTCAGGATCGAGCTGTTCAACAGAATAATCAAACGGTGGGGATGATTAAAACCCTAATATTTCACACTGGAAAATCACCTCGTGGTGATCGAACCGATTGGGTTATGCATGAGTATAGGCTTGAAGATAAGGACTTGGCTGACAAAGGAATTGTTCAGGTAGTCATTTCTTTCTTAGTTGTTTTGTATGAGCTTTATATGGAGATATATGTGTATTGGTTGCCACATATTGAACTGGTGAATACGTGGCAGATTCGTAGTTGTGCCTTTGACTGCGAATGCATATAAGATACCTTTTACTCGCAGTCTTGATACCATTGGCATATATTCCTTTCCAATGTAACAGATAAGACCGGAATATGCTTTTGATGCACAAATTTACATTTGTATTCATGTCTCCACCTATTGTGTCACTAATGCCATTAAAGGACACAACTACTTTAAATGCTAGGGTTAGGGTTATCCTTCGAAGGGTGAAATGTGGAGTTCTAACTTCTAATAATTGATTACAGTGTAGTTCATGATAGTTTTGCATGCAGGATAATCCTTGAAATAGAATTATGTTATTTCATGATAGTTTTACATTACCTTATAAAGTGCATTTTTTACTTTCGAATAGTTGAATTCTTGAAATAGAATTATGCTATTGCATGTTTTGTTTGACTAACACTCAAAATGAGATTTGCTGTTTTAACACGACAAAGTTAGTATCCCTTGCTTTCTTTTAATTATTTGACAGTTTTTTCCATCCTATTCTCGTTGTGAATATGTTTTTAACAAGTTCTCTTGGTATGACAGGATTCCTACGTAATCTGTAAGGTATTTCAAAAAGAAGGTCCTGGTCCTAGGAATGGTGCACAATATGGGAGACCATTTAATGAGGAAGACTGGAGTGATGATGAAGTAGGAATACCTTTTGCTGAATCTGCTGCACTAGTTCCCAGTTTGCCTGTGACATCTAATAGTTCTGTTCTAAATGATCAGAATCTCCAAACTAGTGGATATATTGGTTCGATATCCATACCGTGTCAATCAGAATTGGCGCCTTCTCTGGACCCAACAAATTCATGTCAAACAGGATTGACACCTTCTCCTGACCCTGCAAATCCATGTCAAACAGGATTTATGCCTTATCCAGACCCTGTGAATCCTTGTCAAACAGGAGGATTTATGCCGTCTCCTGACCCAGCAAATTCATGTCAAACAGGATTGACGCCTTCTCCAGACCCTTCAAATTCTTGTCAAACAGGATTTATGCCTTATCCAGACCCTGTGAATTCTTGTCAAACAGGATTTATGCCTGCTTCCGACCCTGCAAATTCATGTCAAACAGAATTTATGCCGTCCCCTGACCTTGCGAGTTCATGTCAAATAGGATTGATGCCTTTGCCCGACCCTGCAAATGATTCATATCCAGACAATCAAGCTGTTAACGACGATGATGATGATGATATTCTAGCAATGCTTGATATTTTCAAAGACAACAGCGTATTGCCTGCGCCCGACCCTGCAAATGATTCATATCCAGACAATCAAACTGTTAATGATGATGATGATATTCTAGCAATGCTTGATATTTTCGAAGACAACGGCGTATTGCCTGAGGTATGCTAATCTGTTTTTTTCTTCTTTCTACTTGTGTTTTTCTGGAAACAGTGTGATGTTTCTTTATTATGTCTGGTTTTAGTATTTGTAGACTTGTTTTATCTATTCCCTGCAGTTTGCATTGTCTCCTCATAATGTTAATGCCATTTTACTAAATCATAGCGATGTATATAGTCCTTGATTTCTTGAAGGATAACTGAGTGTTGACATTATTATTGGTGTCAGGAAAACATTGCTGAAGGTGCACTCTTAACTGACTTTTTTGATGGCTTGGAAGACGTGGATTGCTCGTCTGATTTGGGATCTGTTGGCCAGAATGCAGACTTTAGCACACACGGGCTGGCTTCTACGGGCAATGTTGGGGGGCATGACGACTTCGATTTCATGGAGTTGATTGATCTAGACTCTGATACGTTCTGGCAGACCCAACCTGAAAGTTGGAGTCGAAATAAATAA
Protein sequence

>MS.gene030625.t1

MKRATEIGFWKATGQDRAVQQNNQTVGMIKTLIFHTGKSPRGDRTDWVMHEYRLEDKDLADKGIVQDSYVICKVFQKEGPGPRNGAQYGRPFNEEDWSDDEVGIPFAESAALVPSLPVTSNSSVLNDQNLQTSGYIGSISIPCQSELAPSLDPTNSCQTGLTPSPDPANPCQTGFMPYPDPVNPCQTGGFMPSPDPANSCQTGLTPSPDPSNSCQTGFMPYPDPVNSCQTGFMPASDPANSCQTEFMPSPDLASSCQIGLMPLPDPANDSYPDNQAVNDDDDDDILAMLDIFKDNSVLPAPDPANDSYPDNQTVNDDDDILAMLDIFEDNGVLPEENIAEGALLTDFFDGLEDVDCSSDLGSVGQNADFSTHGLASTGNVGGHDDFDFMELIDLDSDTFWQTQPESWSRNK