AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030646

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030646.t1 MTR_8g093070 95.661 507 22 0 1 507 1 507 0.0 1003
MS.gene030646.t1 MTR_8g092440 86.364 154 19 1 1 154 1 152 4.72e-87 265
MS.gene030646.t1 MTR_3g072710 38.095 399 194 12 136 496 117 500 2.12e-65 220
MS.gene030646.t1 MTR_5g058860 37.811 402 211 14 125 496 105 497 7.11e-64 216
MS.gene030646.t1 MTR_3g065980 33.075 387 216 14 136 495 169 539 1.34e-39 151
MS.gene030646.t1 MTR_7g027190 30.958 407 223 15 126 495 287 672 1.04e-38 150
MS.gene030646.t1 MTR_4g076020 31.081 370 215 11 144 499 89 432 1.11e-36 141
MS.gene030646.t1 MTR_4g102790 29.177 401 233 16 136 504 289 670 4.25e-36 142
MS.gene030646.t1 MTR_7g074650 27.346 373 230 14 144 497 443 793 6.53e-33 133
MS.gene030646.t1 MTR_4g077760 31.283 374 218 16 144 495 198 554 7.29e-31 126
MS.gene030646.t1 MTR_1g096030 30.633 395 226 18 136 495 77 458 5.61e-27 114
MS.gene030646.t1 MTR_1g069725 26.042 384 237 13 135 496 105 463 1.19e-25 110
MS.gene030646.t1 MTR_2g082090 27.604 384 240 16 136 495 209 578 1.44e-25 110
MS.gene030646.t1 MTR_2g034260 26.250 400 252 14 126 495 197 583 2.71e-25 109
MS.gene030646.t1 MTR_4g133660 25.189 397 261 14 123 496 160 543 3.07e-25 109
MS.gene030646.t1 MTR_8g442410 26.823 384 246 13 136 495 152 524 5.16e-25 108
MS.gene030646.t1 MTR_0092s0100 26.542 373 253 9 133 495 369 730 6.47e-25 109
MS.gene030646.t1 MTR_0092s0100 26.542 373 253 9 133 495 366 727 6.99e-25 108
MS.gene030646.t1 MTR_5g094450 25.542 415 268 15 111 495 128 531 1.12e-24 107
MS.gene030646.t1 MTR_4g064150 25.862 406 248 17 127 498 349 735 3.20e-24 107
MS.gene030646.t1 MTR_3g056110 27.157 394 254 16 123 495 160 541 6.66e-24 105
MS.gene030646.t1 MTR_5g097480 27.518 407 253 18 114 495 154 543 7.64e-24 105
MS.gene030646.t1 MTR_5g015490 26.684 386 250 16 138 497 113 491 8.31e-24 104
MS.gene030646.t1 MTR_2g034250 27.160 405 243 16 126 495 197 584 1.01e-23 105
MS.gene030646.t1 MTR_3g089055 26.649 379 244 12 139 495 198 564 1.93e-23 104
MS.gene030646.t1 MTR_3g089055 26.649 379 244 12 139 495 203 569 1.96e-23 104
MS.gene030646.t1 MTR_1g029420 27.368 380 240 14 139 495 225 591 3.87e-23 103
MS.gene030646.t1 MTR_7g062120 25.485 412 259 17 115 498 351 742 4.41e-23 103
MS.gene030646.t1 MTR_2g034280 26.980 404 242 17 126 495 190 574 4.89e-23 103
MS.gene030646.t1 MTR_2g097410 24.623 398 261 16 127 498 359 743 8.88e-23 102
MS.gene030646.t1 MTR_2g097473 24.474 380 245 16 144 498 294 656 1.10e-22 102
MS.gene030646.t1 MTR_4g064200 25.648 386 244 15 139 498 284 652 1.68e-22 101
MS.gene030646.t1 MTR_2g097467 24.403 377 249 16 144 498 294 656 2.30e-22 101
MS.gene030646.t1 MTR_2g026250 26.257 358 220 15 168 495 252 595 2.50e-22 100
MS.gene030646.t1 MTR_4g097080 26.166 386 246 14 138 497 132 504 4.61e-22 99.8
MS.gene030646.t1 MTR_2g097463 24.359 390 256 17 134 498 282 657 1.82e-21 98.6
MS.gene030646.t1 MTR_4g104020 27.692 390 236 16 136 495 146 519 2.96e-21 97.4
MS.gene030646.t1 MTR_4g064120 24.495 396 248 16 136 498 251 628 9.53e-20 93.2
MS.gene030646.t1 MTR_4g026485 26.702 382 240 16 133 495 263 623 2.64e-18 88.6
MS.gene030646.t1 MTR_4g064180 25.231 325 200 13 136 434 251 558 8.10e-18 87.0
MS.gene030646.t1 MTR_8g077940 27.005 374 247 13 136 495 179 540 8.27e-18 86.7
MS.gene030646.t1 MTR_2g097350 23.426 397 273 13 122 498 257 642 1.15e-17 86.7
MS.gene030646.t1 MTR_4g064160 25.570 395 241 18 136 498 313 686 1.84e-17 85.9
MS.gene030646.t1 MTR_2g097310 23.969 388 262 15 132 498 265 640 3.65e-17 85.1
MS.gene030646.t1 MTR_2g097390 22.727 396 277 14 122 498 258 643 1.62e-16 82.8
MS.gene030646.t1 MTR_7g109580 28.523 298 174 14 219 495 274 553 7.58e-16 80.5
MS.gene030646.t1 MTR_5g015950 22.248 427 252 12 139 498 35 448 5.78e-15 77.4
MS.gene030646.t1 MTR_3g053270 22.248 427 252 12 139 498 35 448 5.78e-15 77.4
MS.gene030646.t1 MTR_1g086970 25.062 403 251 17 126 495 93 477 9.52e-15 77.0
MS.gene030646.t1 MTR_2g099110 24.444 360 216 14 177 497 110 452 2.75e-14 75.5
MS.gene030646.t1 MTR_5g009080 29.834 181 116 7 136 310 53 228 1.71e-13 73.2
MS.gene030646.t1 MTR_4g095500 22.565 421 254 14 139 497 59 469 7.65e-13 70.9
MS.gene030646.t1 MTR_2g097380 22.930 314 198 10 203 498 276 563 2.70e-12 69.3
MS.gene030646.t1 MTR_4g076140 28.155 206 134 7 136 332 45 245 2.77e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030646.t1 AT4G08250 32.905 389 228 14 136 501 104 482 2.60e-47 171
MS.gene030646.t1 AT1G55580 35.238 315 162 12 209 495 144 444 3.10e-42 156
MS.gene030646.t1 AT5G41920 32.275 378 220 14 136 495 40 399 2.14e-40 150
MS.gene030646.t1 AT3G03450 29.973 377 235 13 136 495 180 544 7.19e-37 143
MS.gene030646.t1 AT1G66350 29.178 377 227 14 136 495 152 505 2.58e-35 138
MS.gene030646.t1 AT5G17490 30.311 386 230 14 130 495 149 515 1.14e-34 137
MS.gene030646.t1 AT2G01570 29.351 385 222 16 136 495 221 580 2.07e-32 131
MS.gene030646.t1 AT1G14920 28.424 387 223 16 136 495 169 528 1.81e-31 127
MS.gene030646.t1 AT3G54220 27.394 376 229 14 144 497 298 651 7.76e-31 127
MS.gene030646.t1 AT1G63100 25.000 404 251 14 129 496 242 629 4.40e-30 124
MS.gene030646.t1 AT5G66770 29.738 343 203 12 177 495 255 583 7.60e-30 123
MS.gene030646.t1 AT1G63100 25.000 404 251 14 129 496 267 654 9.61e-30 123
MS.gene030646.t1 AT5G59450 27.475 404 241 18 136 506 224 608 1.00e-29 123
MS.gene030646.t1 AT3G50650 29.661 354 211 14 167 495 201 541 1.14e-28 119
MS.gene030646.t1 AT4G17230 29.073 399 238 16 128 497 144 526 1.58e-28 119
MS.gene030646.t1 AT4G17230 29.073 399 238 16 128 497 144 526 1.58e-28 119
MS.gene030646.t1 AT2G04890 28.465 404 244 18 119 495 27 412 1.30e-27 115
MS.gene030646.t1 AT3G46600 27.423 423 256 16 103 497 181 580 9.27e-27 114
MS.gene030646.t1 AT3G46600 28.278 389 233 15 138 498 3 373 1.02e-26 112
MS.gene030646.t1 AT1G50600 26.166 386 257 13 129 495 149 525 1.17e-26 113
MS.gene030646.t1 AT3G46600 27.423 423 256 16 103 497 149 548 1.18e-26 114
MS.gene030646.t1 AT1G50600 26.166 386 257 13 129 495 220 596 2.31e-26 113
MS.gene030646.t1 AT1G50600 26.166 386 257 13 129 495 224 600 2.45e-26 113
MS.gene030646.t1 AT5G48150 27.833 406 247 16 118 495 102 489 3.06e-26 112
MS.gene030646.t1 AT5G48150 27.833 406 247 16 118 495 102 489 3.06e-26 112
MS.gene030646.t1 AT5G48150 27.833 406 247 16 118 495 102 489 3.06e-26 112
MS.gene030646.t1 AT5G48150 27.833 406 247 16 118 495 102 489 3.06e-26 112
MS.gene030646.t1 AT1G07530 26.698 427 253 18 104 498 369 767 3.62e-25 110
MS.gene030646.t1 AT1G21450 27.586 435 269 18 87 495 178 592 1.16e-24 108
MS.gene030646.t1 AT4G37650 26.531 392 250 14 139 498 146 531 4.76e-24 105
MS.gene030646.t1 AT2G29060 26.617 402 237 20 132 497 314 693 1.30e-22 102
MS.gene030646.t1 AT2G29060 26.617 402 237 20 132 497 277 656 1.33e-22 102
MS.gene030646.t1 AT2G37650 24.490 392 263 12 127 497 335 714 3.37e-21 97.8
MS.gene030646.t1 AT2G37650 24.490 392 263 12 127 497 323 702 3.46e-21 97.8
MS.gene030646.t1 AT4G00150 27.851 377 229 17 135 495 204 553 5.52e-20 93.6
MS.gene030646.t1 AT1G07520 23.907 389 256 14 139 498 318 695 6.33e-18 87.4
MS.gene030646.t1 AT1G07520 23.907 389 256 14 139 498 319 696 6.62e-18 87.4
MS.gene030646.t1 AT4G36710 27.100 369 249 14 138 495 125 484 9.91e-18 86.3
MS.gene030646.t1 AT1G07520 23.907 389 256 14 139 498 154 531 1.03e-17 86.7
MS.gene030646.t1 AT1G07520 23.907 389 256 14 139 498 154 531 1.03e-17 86.7
MS.gene030646.t1 AT1G50420 23.733 434 248 16 136 495 54 478 4.24e-17 84.3
MS.gene030646.t1 AT2G29065 24.768 323 201 12 203 498 323 630 4.70e-17 84.7
MS.gene030646.t1 AT5G52510 26.667 315 191 11 212 495 334 639 6.33e-17 84.3
MS.gene030646.t1 AT2G45160 24.365 394 226 17 141 495 278 638 9.43e-16 80.5
MS.gene030646.t1 AT3G49950 23.846 390 256 12 139 497 30 409 2.05e-15 78.6
MS.gene030646.t1 AT3G60630 24.451 319 184 12 209 495 328 621 2.40e-13 73.2

Find 77 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATACCTCTTTCTCTATA+AGG 0.158083 4.3:+8558693 None:intergenic
ATTTCATCTAGGATTGTATT+TGG 0.213674 4.3:+8559799 None:intergenic
ATTTCGTCTAGGATTGTATT+TGG 0.226044 4.3:+8559739 None:intergenic
CTAAACAATGCCTCGTCTTT+AGG 0.252519 4.3:-8559433 MS.gene030646:CDS
GGAAGTTCAAGAAGAGTTAA+TGG 0.258644 4.3:-8559545 MS.gene030646:CDS
CTGTGATTGCTGTTACTCTA+AGG 0.272598 4.3:+8559107 None:intergenic
GCTTGTTTGGTAAAGGGTAT+TGG 0.274576 4.3:-8558523 MS.gene030646:CDS
AAGCTATTGGTTAGCTTGTT+TGG 0.278423 4.3:-8558536 MS.gene030646:CDS
AACAAGCCTTGGTGATGATT+TGG 0.290948 4.3:+8558862 None:intergenic
CTTTGACTTCTGTTTCAATC+TGG 0.297827 4.3:-8558454 MS.gene030646:CDS
CTTGTTTGGTAAAGGGTATT+GGG 0.313644 4.3:-8558522 MS.gene030646:CDS
CCAGGGAAGAAGATTCTTAT+TGG 0.318155 4.3:-8559570 MS.gene030646:CDS
GCGAAAATGTGTTTGTTTCC+AGG 0.325069 4.3:-8559588 MS.gene030646:CDS
CAATACCTCTTTCTCTATAA+GGG 0.331359 4.3:+8558694 None:intergenic
TGTGGTGGAGTTACCGAAAA+AGG 0.334912 4.3:-8559613 MS.gene030646:CDS
TATTTCACAGGCTAAGCTAT+TGG 0.357597 4.3:-8558549 MS.gene030646:CDS
CTCATAATCCATCACATTCA+TGG 0.366173 4.3:+8559918 None:intergenic
TGGTTAGCTTGTTTGGTAAA+GGG 0.385802 4.3:-8558529 MS.gene030646:CDS
TAAGGGCTAACTCCATCTTA+TGG 0.393772 4.3:+8558711 None:intergenic
AAGGCGATAGTTTATTGAAC+AGG 0.393824 4.3:-8559408 MS.gene030646:CDS
CCTTATAGAGAAAGAGGTAT+TGG 0.394224 4.3:-8558693 MS.gene030646:CDS
GATCAAACAATATTTCATCT+AGG 0.398157 4.3:+8559788 None:intergenic
AATCCAATGGCCACCGTTGA+TGG 0.402840 4.3:-8559161 MS.gene030646:CDS
TTGGTTAGCTTGTTTGGTAA+AGG 0.407587 4.3:-8558530 MS.gene030646:CDS
ATCAATGAGAAACTCCTGTT+TGG 0.411022 4.3:+8558908 None:intergenic
ACTCAAATCCATGTCCTTTG+TGG 0.411956 4.3:-8558804 MS.gene030646:CDS
TTTGACTTCTGTTTCAATCT+GGG 0.412837 4.3:-8558453 MS.gene030646:CDS
TACATCAGTGGATGCCAAAC+AGG 0.416877 4.3:-8558922 MS.gene030646:CDS
AGCTTCGAAAATCGCTTGAT+TGG 0.418613 4.3:+8559234 None:intergenic
CTAAGCTACATTCATGCAGC+TGG 0.426844 4.3:+8559860 None:intergenic
CTTATAGAGAAAGAGGTATT+GGG 0.435957 4.3:-8558692 MS.gene030646:CDS
GAGATAAGAGATGTTGTGAT+TGG 0.464477 4.3:+8559761 None:intergenic
CAACCATCAACGGTGGCCAT+TGG 0.475147 4.3:+8559158 None:intergenic
CCAATAAGAATCTTCTTCCC+TGG 0.475253 4.3:+8559570 None:intergenic
GAGACATGAGATCTTCTGCT+TGG 0.478088 4.3:+8559821 None:intergenic
CGAAAATGTGTTTGTTTCCA+GGG 0.485498 4.3:-8559587 MS.gene030646:CDS
GTTAGCCCTTATAGAGAAAG+AGG 0.487244 4.3:-8558699 MS.gene030646:CDS
AATGCCTCGTCTTTAGGAAA+AGG 0.493064 4.3:-8559427 MS.gene030646:CDS
TCGACTAAAACAACAAGCCT+TGG 0.505560 4.3:+8558851 None:intergenic
ACATGTTCTGAATGGAACTT+AGG 0.509744 4.3:+8559337 None:intergenic
GCAATATGAGAACTGTAAGT+TGG 0.510443 4.3:-8558498 MS.gene030646:CDS
CATGTTCGATACAGTAATGA+TGG 0.510797 4.3:-8559005 MS.gene030646:CDS
AAAAGAGGAGAGAATATTGT+GGG 0.510893 4.3:-8558630 MS.gene030646:CDS
GAAGCCTCAGTTCAACAAAC+AGG 0.521298 4.3:-8559070 MS.gene030646:CDS
TTACTCATATTATAATCTGT+TGG 0.522232 4.3:+8558428 None:intergenic
ACTGTAAGTTGGCTTTGTGT+TGG 0.525441 4.3:-8558487 MS.gene030646:CDS
GAATTCAAAGAAATTGAACT+TGG 0.527275 4.3:-8558971 MS.gene030646:CDS
GGAAGAAGATTCTTATTGGA+AGG 0.535054 4.3:-8559566 MS.gene030646:CDS
GTGTAGAAACATGTTCTGAA+TGG 0.541579 4.3:+8559329 None:intergenic
CTTGATCGAAGATTTCGTCT+AGG 0.546863 4.3:+8559728 None:intergenic
GCAGCTGGTTCATGCAAGGA+GGG 0.553162 4.3:+8559875 None:intergenic
AGATCTTCTGCTTGGAGTGG+TGG 0.557382 4.3:+8559829 None:intergenic
TTGCTGCAGCAATTCTTGCA+TGG 0.560772 4.3:+8559699 None:intergenic
GATTCATGATATTGAAGTCG+AGG 0.561299 4.3:+8559668 None:intergenic
CATGAATCATGAACAAGCAA+TGG 0.561832 4.3:-8559653 MS.gene030646:CDS
TCTGTGGAGTAGCCATAAGA+TGG 0.563300 4.3:-8558723 MS.gene030646:CDS
TTGAAGGGGTATAGACGTGT+TGG 0.563890 4.3:-8558587 MS.gene030646:CDS
ACTATCTACCATGAATGTGA+TGG 0.583158 4.3:-8559926 None:intergenic
TCTGCCTGTTTGTTGAACTG+AGG 0.584240 4.3:+8559066 None:intergenic
TGTGATTGCTGTTACTCTAA+GGG 0.585072 4.3:+8559108 None:intergenic
GAGTACATGCAATATTTCAC+AGG 0.587078 4.3:-8558561 MS.gene030646:CDS
CAGTTCAACAAACAGGCAGA+AGG 0.599250 4.3:-8559063 MS.gene030646:CDS
TCATGCAGCTGGTTCATGCA+AGG 0.599943 4.3:+8559871 None:intergenic
TGCAGCTGGTTCATGCAAGG+AGG 0.602202 4.3:+8559874 None:intergenic
ATTGCAAAGTCAACCATCAA+CGG 0.607337 4.3:+8559148 None:intergenic
CAGATTGAAACAGAAGTCAA+AGG 0.620209 4.3:+8558455 None:intergenic
ATTACTGTATCGAACATGAA+CGG 0.648207 4.3:+8559010 None:intergenic
ATGAGATCTTCTGCTTGGAG+TGG 0.651380 4.3:+8559826 None:intergenic
ATATCATGGAGGGAATCCAA+TGG 0.669550 4.3:-8559174 MS.gene030646:CDS
CTAGAGAATGAATTGTGTGG+TGG 0.675354 4.3:-8559628 MS.gene030646:CDS
CAGCTGGTTCATGCAAGGAG+GGG 0.677543 4.3:+8559876 None:intergenic
ATTCACTTGTAAGTACTCTG+TGG 0.679195 4.3:-8558739 MS.gene030646:CDS
TCAATTGTATGATACATCAG+TGG 0.691913 4.3:-8558934 MS.gene030646:CDS
GCAAAGTCAACCATCAACGG+TGG 0.711871 4.3:+8559151 None:intergenic
ACGTAACCAAATCATCACCA+AGG 0.735440 4.3:-8558868 MS.gene030646:CDS
TAGAGTAACAGCAATCACAG+TGG 0.741841 4.3:-8559104 MS.gene030646:CDS
GCTCTAGAGAATGAATTGTG+TGG 0.769887 4.3:-8559631 MS.gene030646:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTTTTTTATTCATTGA+AGG - chr4.3:8559728-8559747 MS.gene030646:CDS 15.0%
!!! AGATTTTTTTATTCATTGAA+GGG - chr4.3:8559729-8559748 MS.gene030646:CDS 15.0%
!! TTACTCATATTATAATCTGT+TGG + chr4.3:8559906-8559925 None:intergenic 20.0%
!!! AAAATGAGCAAATTTTACAT+AGG + chr4.3:8559073-8559092 None:intergenic 20.0%
!!! AAATGAGCAAATTTTACATA+GGG + chr4.3:8559072-8559091 None:intergenic 20.0%
!!! GATTTTTTTATTCATTGAAG+GGG - chr4.3:8559730-8559749 MS.gene030646:CDS 20.0%
! GAATTCAAAGAAATTGAACT+TGG - chr4.3:8559360-8559379 MS.gene030646:CDS 25.0%
! GATCAAACAATATTTCATCT+AGG + chr4.3:8558546-8558565 None:intergenic 25.0%
!! ATTTCATCTAGGATTGTATT+TGG + chr4.3:8558535-8558554 None:intergenic 25.0%
!!! ATTGATTTTGATATCATGGA+GGG - chr4.3:8559147-8559166 MS.gene030646:CDS 25.0%
!!! TGTCATTGATTTTGATATCA+TGG - chr4.3:8559143-8559162 MS.gene030646:CDS 25.0%
AAAAAGAGGAGAGAATATTG+TGG - chr4.3:8559700-8559719 MS.gene030646:CDS 30.0%
AAAAGAGGAGAGAATATTGT+GGG - chr4.3:8559701-8559720 MS.gene030646:CDS 30.0%
ATTACTGTATCGAACATGAA+CGG + chr4.3:8559324-8559343 None:intergenic 30.0%
CAATACCTCTTTCTCTATAA+GGG + chr4.3:8559640-8559659 None:intergenic 30.0%
TCAATTGTATGATACATCAG+TGG - chr4.3:8559397-8559416 MS.gene030646:CDS 30.0%
! ATTTCGTCTAGGATTGTATT+TGG + chr4.3:8558595-8558614 None:intergenic 30.0%
! TTAGTTGATCTTTTGCTGAT+AGG - chr4.3:8558820-8558839 MS.gene030646:CDS 30.0%
! TTTGACTTCTGTTTCAATCT+GGG - chr4.3:8559878-8559897 MS.gene030646:CDS 30.0%
!! CTTATAGAGAAAGAGGTATT+GGG - chr4.3:8559639-8559658 MS.gene030646:CDS 30.0%
!!! CATTGATTTTGATATCATGG+AGG - chr4.3:8559146-8559165 MS.gene030646:CDS 30.0%
AAGGCGATAGTTTATTGAAC+AGG - chr4.3:8558923-8558942 MS.gene030646:CDS 35.0%
ACAGTTTCCTTGTGAAAAAG+AGG - chr4.3:8559686-8559705 MS.gene030646:CDS 35.0%
ATCAATGAGAAACTCCTGTT+TGG + chr4.3:8559426-8559445 None:intergenic 35.0%
ATTCACTTGTAAGTACTCTG+TGG - chr4.3:8559592-8559611 MS.gene030646:CDS 35.0%
ATTGCAAAGTCAACCATCAA+CGG + chr4.3:8559186-8559205 None:intergenic 35.0%
CAGATTGAAACAGAAGTCAA+AGG + chr4.3:8559879-8559898 None:intergenic 35.0%
CATGAATCATGAACAAGCAA+TGG - chr4.3:8558678-8558697 MS.gene030646:CDS 35.0%
CATGTTCGATACAGTAATGA+TGG - chr4.3:8559326-8559345 MS.gene030646:CDS 35.0%
CCAATACCTCTTTCTCTATA+AGG + chr4.3:8559641-8559660 None:intergenic 35.0%
CGAAAATGTGTTTGTTTCCA+GGG - chr4.3:8558744-8558763 MS.gene030646:CDS 35.0%
GAGATAAGAGATGTTGTGAT+TGG + chr4.3:8558573-8558592 None:intergenic 35.0%
GAGTACATGCAATATTTCAC+AGG - chr4.3:8559770-8559789 MS.gene030646:CDS 35.0%
GATTCATGATATTGAAGTCG+AGG + chr4.3:8558666-8558685 None:intergenic 35.0%
GCAATATGAGAACTGTAAGT+TGG - chr4.3:8559833-8559852 MS.gene030646:CDS 35.0%
GGAAGAAGATTCTTATTGGA+AGG - chr4.3:8558765-8558784 MS.gene030646:CDS 35.0%
GGAAGTTCAAGAAGAGTTAA+TGG - chr4.3:8558786-8558805 MS.gene030646:CDS 35.0%
TATTTCACAGGCTAAGCTAT+TGG - chr4.3:8559782-8559801 MS.gene030646:CDS 35.0%
! ACATGTTCTGAATGGAACTT+AGG + chr4.3:8558997-8559016 None:intergenic 35.0%
! CCTTATAGAGAAAGAGGTAT+TGG - chr4.3:8559638-8559657 MS.gene030646:CDS 35.0%
! CTTTGACTTCTGTTTCAATC+TGG - chr4.3:8559877-8559896 MS.gene030646:CDS 35.0%
! GTGTAGAAACATGTTCTGAA+TGG + chr4.3:8559005-8559024 None:intergenic 35.0%
! TGGAAACAAACACATTTTCG+CGG + chr4.3:8558744-8558763 None:intergenic 35.0%
! TGTGATTGCTGTTACTCTAA+GGG + chr4.3:8559226-8559245 None:intergenic 35.0%
! TTGTGTCTTTTCTTTACGCA+AGG - chr4.3:8558946-8558965 MS.gene030646:CDS 35.0%
!! AAGCTATTGGTTAGCTTGTT+TGG - chr4.3:8559795-8559814 MS.gene030646:CDS 35.0%
!! ATTCTCTCCTCTTTTTCACA+AGG + chr4.3:8559696-8559715 None:intergenic 35.0%
!! CTTGTTTGGTAAAGGGTATT+GGG - chr4.3:8559809-8559828 MS.gene030646:CDS 35.0%
!! TGGTTAGCTTGTTTGGTAAA+GGG - chr4.3:8559802-8559821 MS.gene030646:CDS 35.0%
!! TTGGTTAGCTTGTTTGGTAA+AGG - chr4.3:8559801-8559820 MS.gene030646:CDS 35.0%
ACGTAACCAAATCATCACCA+AGG - chr4.3:8559463-8559482 MS.gene030646:CDS 40.0%
ACTCAAATCCATGTCCTTTG+TGG - chr4.3:8559527-8559546 MS.gene030646:CDS 40.0%
AGCTTCGAAAATCGCTTGAT+TGG + chr4.3:8559100-8559119 None:intergenic 40.0%
ATATCATGGAGGGAATCCAA+TGG - chr4.3:8559157-8559176 MS.gene030646:CDS 40.0%
CCAATAAGAATCTTCTTCCC+TGG + chr4.3:8558764-8558783 None:intergenic 40.0%
CCAGGGAAGAAGATTCTTAT+TGG - chr4.3:8558761-8558780 MS.gene030646:CDS 40.0%
CTAAACAATGCCTCGTCTTT+AGG - chr4.3:8558898-8558917 MS.gene030646:CDS 40.0%
CTAGAGAATGAATTGTGTGG+TGG - chr4.3:8558703-8558722 MS.gene030646:CDS 40.0%
CTCACAGAAAAACTCCACAA+AGG + chr4.3:8559544-8559563 None:intergenic 40.0%
CTTGATCGAAGATTTCGTCT+AGG + chr4.3:8558606-8558625 None:intergenic 40.0%
GAAAAACTCCACAAAGGACA+TGG + chr4.3:8559538-8559557 None:intergenic 40.0%
GCGAAAATGTGTTTGTTTCC+AGG - chr4.3:8558743-8558762 MS.gene030646:CDS 40.0%
GTTAGCCCTTATAGAGAAAG+AGG - chr4.3:8559632-8559651 MS.gene030646:CDS 40.0%
TAAGGGCTAACTCCATCTTA+TGG + chr4.3:8559623-8559642 None:intergenic 40.0%
TAGAGTAACAGCAATCACAG+TGG - chr4.3:8559227-8559246 MS.gene030646:CDS 40.0%
TCGACTAAAACAACAAGCCT+TGG + chr4.3:8559483-8559502 None:intergenic 40.0%
! AATGCCTCGTCTTTAGGAAA+AGG - chr4.3:8558904-8558923 MS.gene030646:CDS 40.0%
! CTGTGATTGCTGTTACTCTA+AGG + chr4.3:8559227-8559246 None:intergenic 40.0%
! CTTTGTGGAGTTTTTCTGTG+AGG - chr4.3:8559542-8559561 MS.gene030646:CDS 40.0%
! GCTTGTTTGGTAAAGGGTAT+TGG - chr4.3:8559808-8559827 MS.gene030646:CDS 40.0%
!! AACAAGCCTTGGTGATGATT+TGG + chr4.3:8559472-8559491 None:intergenic 40.0%
!! ACTGTAAGTTGGCTTTGTGT+TGG - chr4.3:8559844-8559863 MS.gene030646:CDS 40.0%
!! GCTCTAGAGAATGAATTGTG+TGG - chr4.3:8558700-8558719 MS.gene030646:CDS 40.0%
ATGAGATCTTCTGCTTGGAG+TGG + chr4.3:8558508-8558527 None:intergenic 45.0%
CAGTTCAACAAACAGGCAGA+AGG - chr4.3:8559268-8559287 MS.gene030646:CDS 45.0%
CTAAGCTACATTCATGCAGC+TGG + chr4.3:8558474-8558493 None:intergenic 45.0%
GAAGCCTCAGTTCAACAAAC+AGG - chr4.3:8559261-8559280 MS.gene030646:CDS 45.0%
GAGACATGAGATCTTCTGCT+TGG + chr4.3:8558513-8558532 None:intergenic 45.0%
TACATCAGTGGATGCCAAAC+AGG - chr4.3:8559409-8559428 MS.gene030646:CDS 45.0%
TCTGTGGAGTAGCCATAAGA+TGG - chr4.3:8559608-8559627 MS.gene030646:CDS 45.0%
TTGCTGCAGCAATTCTTGCA+TGG + chr4.3:8558635-8558654 None:intergenic 45.0%
! ATCGCCTTTTCCTAAAGACG+AGG + chr4.3:8558911-8558930 None:intergenic 45.0%
! CACATTTTCGCGGCCTTTTT+CGG + chr4.3:8558734-8558753 None:intergenic 45.0%
! TCTGCCTGTTTGTTGAACTG+AGG + chr4.3:8559268-8559287 None:intergenic 45.0%
! TGTGGTGGAGTTACCGAAAA+AGG - chr4.3:8558718-8558737 MS.gene030646:CDS 45.0%
! TTGAAGGGGTATAGACGTGT+TGG - chr4.3:8559744-8559763 MS.gene030646:CDS 45.0%
AATCCAATGGCCACCGTTGA+TGG - chr4.3:8559170-8559189 MS.gene030646:CDS 50.0%
AGATCTTCTGCTTGGAGTGG+TGG + chr4.3:8558505-8558524 None:intergenic 50.0%
GCAAAGTCAACCATCAACGG+TGG + chr4.3:8559183-8559202 None:intergenic 50.0%
! GCGATTTTCGAAGCTACAGC+TGG - chr4.3:8559105-8559124 MS.gene030646:CDS 50.0%
! TCATGCAGCTGGTTCATGCA+AGG + chr4.3:8558463-8558482 None:intergenic 50.0%
CAACCATCAACGGTGGCCAT+TGG + chr4.3:8559176-8559195 None:intergenic 55.0%
! CAGCTGGTTCATGCAAGGAG+GGG + chr4.3:8558458-8558477 None:intergenic 55.0%
! GCAGCTGGTTCATGCAAGGA+GGG + chr4.3:8558459-8558478 None:intergenic 55.0%
! TGCAGCTGGTTCATGCAAGG+AGG + chr4.3:8558460-8558479 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 8558415 8559938 8558415 ID=MS.gene030646
chr4.3 mRNA 8558415 8559938 8558415 ID=MS.gene030646.t1;Parent=MS.gene030646
chr4.3 exon 8558415 8559938 8558415 ID=MS.gene030646.t1.exon1;Parent=MS.gene030646.t1
chr4.3 CDS 8558415 8559938 8558415 ID=cds.MS.gene030646.t1;Parent=MS.gene030646.t1
Gene Sequence

>MS.gene030646

ATGAATGTGATGGATTATGAGCAATTTGATTACTCATTCACCCCTCCTTGCATGAACCAGCTGCATGAATGTAGCTTAGAAAATTTACCACCACTCCAAGCAGAAGATCTCATGTCTCCAAATACAATCCTAGATGAAATATTGTTTGATCAAGACCAATCACAACATCTCTTATCTCCAAATACAATCCTAGACGAAATCTTCGATCAAGAAGAGTCCATGCAAGAATTGCTGCAGCAACATACAAACCTCGACTTCAATATCATGAATCATGAACAAGCAATGGCTCTAGAGAATGAATTGTGTGGTGGAGTTACCGAAAAAGGCCGCGAAAATGTGTTTGTTTCCAGGGAAGAAGATTCTTATTGGAAGGAAGTTCAAGAAGAGTTAATGGAAGAGACAAGTTTAGTTGATCTTTTGCTGATAGGAGCTGAAGCTGCTGAATCACAAAACATGACTCTTGCTTCTGACATAATCGAAAAACTAAACAATGCCTCGTCTTTAGGAAAAGGCGATAGTTTATTGAACAGGTTGTGTCTTTTCTTTACGCAAGGATTGTATTATAAAACTACAAATGCTCCTAAGTTCCATTCAGAACATGTTTCTACACAGACAAGCACATTCTGTGTGTTTCAGATACTTCAAGAACTCTCTCCCTATGTAAAATTTGCTCATTTTACTGCCAATCAAGCGATTTTCGAAGCTACAGCTGGTGTCGAAGATGTTCATGTCATTGATTTTGATATCATGGAGGGAATCCAATGGCCACCGTTGATGGTTGACTTTGCAATGAGAAAGAAGACTACTTCCCTTAGAGTAACAGCAATCACAGTGGATCAGCAAAGTGAAGCCTCAGTTCAACAAACAGGCAGAAGGCTCAAAGAGTTCGCTGATTCAATCAATTTTCCGTTCATGTTCGATACAGTAATGATGGTGAGTGAAGAAGAATTCAAAGAAATTGAACTTGGTGAAACATTTATTGTCAATTGTATGATACATCAGTGGATGCCAAACAGGAGTTTCTCATTGATCAAAGCTTTTCTCGATCACGTAACCAAATCATCACCAAGGCTTGTTGTTTTAGTCGAAGAAGAACTGTTTAATTTTTCTAGACTCAAATCCATGTCCTTTGTGGAGTTTTTCTGTGAGGCTTTGCATCACTATATTGCAGTTTCTGATTCACTTGTAAGTACTCTGTGGAGTAGCCATAAGATGGAGTTAGCCCTTATAGAGAAAGAGGTATTGGGAAATAGAATCTTAGACAGTGTAAGACAGTTTCCTTGTGAAAAAGAGGAGAGAATATTGTGGGAAGAAAGATTTTTTTATTCATTGAAGGGGTATAGACGTGTTGGTATGAGTACATGCAATATTTCACAGGCTAAGCTATTGGTTAGCTTGTTTGGTAAAGGGTATTGGGTGCAATATGAGAACTGTAAGTTGGCTTTGTGTTGGAAGTCAAGACCTTTGACTTCTGTTTCAATCTGGGTACCAACAGATTATAATATGAGTAACAAAGTCAAATAG
Protein sequence

>MS.gene030646.t1

MNVMDYEQFDYSFTPPCMNQLHECSLENLPPLQAEDLMSPNTILDEILFDQDQSQHLLSPNTILDEIFDQEESMQELLQQHTNLDFNIMNHEQAMALENELCGGVTEKGRENVFVSREEDSYWKEVQEELMEETSLVDLLLIGAEAAESQNMTLASDIIEKLNNASSLGKGDSLLNRLCLFFTQGLYYKTTNAPKFHSEHVSTQTSTFCVFQILQELSPYVKFAHFTANQAIFEATAGVEDVHVIDFDIMEGIQWPPLMVDFAMRKKTTSLRVTAITVDQQSEASVQQTGRRLKEFADSINFPFMFDTVMMVSEEEFKEIELGETFIVNCMIHQWMPNRSFSLIKAFLDHVTKSSPRLVVLVEEELFNFSRLKSMSFVEFFCEALHHYIAVSDSLVSTLWSSHKMELALIEKEVLGNRILDSVRQFPCEKEERILWEERFFYSLKGYRRVGMSTCNISQAKLLVSLFGKGYWVQYENCKLALCWKSRPLTSVSIWVPTDYNMSNKVK