Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030712.t1 | XP_003630118.1 | 75.6 | 127 | 23 | 1 | 1 | 119 | 1 | 127 | 1.80E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030712.t1 | Q93WU9 | 32.5 | 126 | 72 | 3 | 6 | 118 | 3 | 128 | 1.5e-09 | 63.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030712.t1 | A0A072TVK6 | 75.6 | 127 | 23 | 1 | 1 | 119 | 1 | 127 | 1.3e-43 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene030712.t1 | TF | WRKY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030712.t1 | MTR_8g092010 | 88.189 | 127 | 7 | 1 | 1 | 119 | 1 | 127 | 5.13e-78 | 226 |
| MS.gene030712.t1 | MTR_8g092010 | 88.189 | 127 | 7 | 1 | 1 | 119 | 1 | 127 | 2.61e-77 | 226 |
| MS.gene030712.t1 | MTR_3g095040 | 49.275 | 138 | 46 | 8 | 1 | 117 | 1 | 135 | 4.90e-25 | 94.7 |
| MS.gene030712.t1 | MTR_1g015140 | 37.168 | 113 | 55 | 3 | 13 | 118 | 17 | 120 | 1.74e-13 | 63.5 |
| MS.gene030712.t1 | MTR_5g029850 | 50.877 | 57 | 22 | 1 | 67 | 117 | 12 | 68 | 1.79e-12 | 58.9 |
| MS.gene030712.t1 | MTR_3g104750 | 75.000 | 32 | 8 | 0 | 87 | 118 | 91 | 122 | 3.66e-12 | 60.1 |
| MS.gene030712.t1 | MTR_4g013260 | 32.967 | 91 | 59 | 1 | 28 | 118 | 266 | 354 | 8.46e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030712.t1 | AT5G64810 | 34.127 | 126 | 70 | 3 | 6 | 118 | 3 | 128 | 6.69e-13 | 62.8 |
| MS.gene030712.t1 | AT5G26170 | 71.875 | 32 | 9 | 0 | 87 | 118 | 100 | 131 | 4.07e-12 | 60.5 |
| MS.gene030712.t1 | AT2G46130 | 46.154 | 52 | 26 | 1 | 79 | 128 | 9 | 60 | 1.68e-11 | 57.4 |
| MS.gene030712.t1 | AT2G46130 | 60.526 | 38 | 15 | 0 | 79 | 116 | 9 | 46 | 1.82e-11 | 57.0 |
Find 24 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGAACAAAGTAGCTTTA+AGG | 0.256918 | 4.3:+9340262 | MS.gene030712:intron |
| GAAGTAGAGTGAGCAAAATT+TGG | 0.273680 | 4.3:-9339481 | None:intergenic |
| AAGATCAGAGCTTGAGATTA+TGG | 0.274000 | 4.3:+9340119 | MS.gene030712:CDS |
| CTTGTTCTAAAGGCTATCTT+TGG | 0.312484 | 4.3:-9340099 | None:intergenic |
| TGCATCATTGAATTCTTGAT+TGG | 0.365316 | 4.3:-9339617 | None:intergenic |
| AAATGGGATTAAGCGAAATA+AGG | 0.381517 | 4.3:+9340065 | MS.gene030712:CDS |
| TTACATATTATTGTTCTTTG+AGG | 0.400718 | 4.3:-9339641 | None:intergenic |
| TTGCTTGATGATATTTGTAT+TGG | 0.407278 | 4.3:+9339529 | MS.gene030712:CDS |
| TGTTACCTTCTCTAATGATT+CGG | 0.455426 | 4.3:-9339584 | None:intergenic |
| GGATACAAGTGGAGGAAGTA+CGG | 0.474552 | 4.3:+9340147 | MS.gene030712:CDS |
| AAGCTCTGATCTTGTTCTAA+AGG | 0.476991 | 4.3:-9340109 | None:intergenic |
| GTGACTGAGATTCTTGATCA+AGG | 0.485292 | 4.3:-9339555 | None:intergenic |
| GAGCTTGAGATTATGGATGA+TGG | 0.492012 | 4.3:+9340126 | MS.gene030712:CDS |
| AAGCGAAATAAGGTAGAAGC+GGG | 0.536791 | 4.3:+9340075 | MS.gene030712:CDS |
| CAAAATTTGGAGTAGCATGA+GGG | 0.551934 | 4.3:-9339468 | None:intergenic |
| GATACAAGTGGAGGAAGTAC+GGG | 0.554483 | 4.3:+9340148 | MS.gene030712:CDS |
| TGAAGAACAGTCCTAACCTA+AGG | 0.563814 | 4.3:+9340181 | MS.gene030712:CDS |
| TGAACAAAGTAGCTTTAAGG+TGG | 0.569106 | 4.3:+9340265 | MS.gene030712:intron |
| GCAAAATTTGGAGTAGCATG+AGG | 0.589246 | 4.3:-9339469 | None:intergenic |
| TTATGGATGATGGATACAAG+TGG | 0.599156 | 4.3:+9340136 | MS.gene030712:CDS |
| AAGTACCGAATCATTAGAGA+AGG | 0.606756 | 4.3:+9339579 | MS.gene030712:CDS |
| TAAGCGAAATAAGGTAGAAG+CGG | 0.616655 | 4.3:+9340074 | MS.gene030712:CDS |
| TGGATGATGGATACAAGTGG+AGG | 0.653822 | 4.3:+9340139 | MS.gene030712:CDS |
| AGCGAAATAAGGTAGAAGCG+GGG | 0.727454 | 4.3:+9340076 | MS.gene030712:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCATTTAAAATTAAGAAAA+GGG | + | chr4.3:9339695-9339714 | MS.gene030712:intron | 10.0% |
| !! | CAAATACAAAATGAAATTTA+AGG | - | chr4.3:9340247-9340266 | None:intergenic | 15.0% |
| !!! | GTTCATTTAAAATTAAGAAA+AGG | + | chr4.3:9339694-9339713 | MS.gene030712:intron | 15.0% |
| !! | TTACATATTATTGTTCTTTG+AGG | - | chr4.3:9339644-9339663 | None:intergenic | 20.0% |
| !! | TTTGCAGAAAATACAAAAAT+GGG | + | chr4.3:9340049-9340068 | MS.gene030712:intron | 20.0% |
| !!! | CTAATAAAGTGTACATATTT+TGG | + | chr4.3:9340008-9340027 | MS.gene030712:intron | 20.0% |
| !!! | TAAAAAACATCATTTTGTTC+AGG | - | chr4.3:9339804-9339823 | None:intergenic | 20.0% |
| !!! | TTTTGCAGAAAATACAAAAA+TGG | + | chr4.3:9340048-9340067 | MS.gene030712:intron | 20.0% |
| ! | AACAAGAGCAATACAAATTA+AGG | - | chr4.3:9339677-9339696 | None:intergenic | 25.0% |
| ! | ACAAGAGCAATACAAATTAA+GGG | - | chr4.3:9339676-9339695 | None:intergenic | 25.0% |
| ! | ATTAATGAAGCATATACCAT+CGG | + | chr4.3:9339939-9339958 | MS.gene030712:intron | 25.0% |
| !! | ATTTGAACAAAGTAGCTTTA+AGG | + | chr4.3:9340262-9340281 | MS.gene030712:intron | 25.0% |
| !! | GTATTTCCTTTTATTACCTT+AGG | - | chr4.3:9340200-9340219 | None:intergenic | 25.0% |
| !! | TCAAAAGTGTACTTCTAAAT+GGG | - | chr4.3:9339757-9339776 | None:intergenic | 25.0% |
| !! | TTCAAAAGTGTACTTCTAAA+TGG | - | chr4.3:9339758-9339777 | None:intergenic | 25.0% |
| !! | TTGCTTGATGATATTTGTAT+TGG | + | chr4.3:9339529-9339548 | MS.gene030712:CDS | 25.0% |
| !!! | TCTAAAAGTGTACATTTTGA+GGG | + | chr4.3:9339827-9339846 | MS.gene030712:intron | 25.0% |
| AAATGGGATTAAGCGAAATA+AGG | + | chr4.3:9340065-9340084 | MS.gene030712:CDS | 30.0% | |
| TCCTAACCTAAGGTAATAAA+AGG | + | chr4.3:9340191-9340210 | MS.gene030712:intron | 30.0% | |
| TGCATCATTGAATTCTTGAT+TGG | - | chr4.3:9339620-9339639 | None:intergenic | 30.0% | |
| TGTTACCTTCTCTAATGATT+CGG | - | chr4.3:9339587-9339606 | None:intergenic | 30.0% | |
| ! | TCCTTTTATTACCTTAGGTT+AGG | - | chr4.3:9340195-9340214 | None:intergenic | 30.0% |
| !! | AGTGTACATATTTTGGATTG+TGG | + | chr4.3:9340015-9340034 | MS.gene030712:intron | 30.0% |
| !! | ATCGGTTTTTAAGAAACTAC+GGG | - | chr4.3:9339734-9339753 | None:intergenic | 30.0% |
| !! | GATCGGTTTTTAAGAAACTA+CGG | - | chr4.3:9339735-9339754 | None:intergenic | 30.0% |
| !! | GTCTAAAAGTGTACATTTTG+AGG | + | chr4.3:9339826-9339845 | MS.gene030712:intron | 30.0% |
| !! | GTGTACATATTTTGGATTGT+GGG | + | chr4.3:9340016-9340035 | MS.gene030712:intron | 30.0% |
| !! | TGTACATATTTTGGATTGTG+GGG | + | chr4.3:9340017-9340036 | MS.gene030712:intron | 30.0% |
| AAGATCAGAGCTTGAGATTA+TGG | + | chr4.3:9340119-9340138 | MS.gene030712:CDS | 35.0% | |
| AGTGTACTTCTAAATGGGAT+CGG | - | chr4.3:9339752-9339771 | None:intergenic | 35.0% | |
| CAAATGAAGTAATGAACCGA+TGG | - | chr4.3:9339958-9339977 | None:intergenic | 35.0% | |
| GAAGTAGAGTGAGCAAAATT+TGG | - | chr4.3:9339484-9339503 | None:intergenic | 35.0% | |
| GCAATACAAATTAAGGGAGA+AGG | - | chr4.3:9339670-9339689 | None:intergenic | 35.0% | |
| TAAGCGAAATAAGGTAGAAG+CGG | + | chr4.3:9340074-9340093 | MS.gene030712:CDS | 35.0% | |
| ! | AAGCTCTGATCTTGTTCTAA+AGG | - | chr4.3:9340112-9340131 | None:intergenic | 35.0% |
| ! | CAAAATTTGGAGTAGCATGA+GGG | - | chr4.3:9339471-9339490 | None:intergenic | 35.0% |
| !! | AAGTACCGAATCATTAGAGA+AGG | + | chr4.3:9339579-9339598 | MS.gene030712:CDS | 35.0% |
| !! | AAGTGTACATTTTGAGGGAT+CGG | + | chr4.3:9339832-9339851 | MS.gene030712:intron | 35.0% |
| !! | CTTGTTCTAAAGGCTATCTT+TGG | - | chr4.3:9340102-9340121 | None:intergenic | 35.0% |
| !! | GGATCGGTTTTTAGAAATCA+TGG | + | chr4.3:9339848-9339867 | MS.gene030712:intron | 35.0% |
| !! | TCGGTTTTTAAGAAACTACG+GGG | - | chr4.3:9339733-9339752 | None:intergenic | 35.0% |
| !! | TGAACAAAGTAGCTTTAAGG+TGG | + | chr4.3:9340265-9340284 | MS.gene030712:intron | 35.0% |
| !! | TTATGGATGATGGATACAAG+TGG | + | chr4.3:9340136-9340155 | MS.gene030712:CDS | 35.0% |
| !!! | TGCATGATTGCGTTTTTTCA+AGG | - | chr4.3:9339888-9339907 | None:intergenic | 35.0% |
| AAGCGAAATAAGGTAGAAGC+GGG | + | chr4.3:9340075-9340094 | MS.gene030712:CDS | 40.0% | |
| GAGCTTGAGATTATGGATGA+TGG | + | chr4.3:9340126-9340145 | MS.gene030712:CDS | 40.0% | |
| GTGACTGAGATTCTTGATCA+AGG | - | chr4.3:9339558-9339577 | None:intergenic | 40.0% | |
| TGAAGAACAGTCCTAACCTA+AGG | + | chr4.3:9340181-9340200 | MS.gene030712:CDS | 40.0% | |
| ! | GCAAAATTTGGAGTAGCATG+AGG | - | chr4.3:9339472-9339491 | None:intergenic | 40.0% |
| AGCGAAATAAGGTAGAAGCG+GGG | + | chr4.3:9340076-9340095 | MS.gene030712:CDS | 45.0% | |
| GGATACAAGTGGAGGAAGTA+CGG | + | chr4.3:9340147-9340166 | MS.gene030712:CDS | 45.0% | |
| ! | GATACAAGTGGAGGAAGTAC+GGG | + | chr4.3:9340148-9340167 | MS.gene030712:CDS | 45.0% |
| !! | TGGATGATGGATACAAGTGG+AGG | + | chr4.3:9340139-9340158 | MS.gene030712:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 9339448 | 9340304 | 9339448 | ID=MS.gene030712 |
| chr4.3 | mRNA | 9339448 | 9340304 | 9339448 | ID=MS.gene030712.t1;Parent=MS.gene030712 |
| chr4.3 | exon | 9339448 | 9339659 | 9339448 | ID=MS.gene030712.t1.exon1;Parent=MS.gene030712.t1 |
| chr4.3 | CDS | 9339448 | 9339659 | 9339448 | ID=cds.MS.gene030712.t1;Parent=MS.gene030712.t1 |
| chr4.3 | exon | 9340056 | 9340202 | 9340056 | ID=MS.gene030712.t1.exon2;Parent=MS.gene030712.t1 |
| chr4.3 | CDS | 9340056 | 9340202 | 9340056 | ID=cds.MS.gene030712.t1;Parent=MS.gene030712.t1 |
| chr4.3 | exon | 9340277 | 9340304 | 9340277 | ID=MS.gene030712.t1.exon3;Parent=MS.gene030712.t1 |
| chr4.3 | CDS | 9340277 | 9340304 | 9340277 | ID=cds.MS.gene030712.t1;Parent=MS.gene030712.t1 |
| Gene Sequence |
| Protein sequence |
