AlfalfaGEDB | MS.gene031244

M. sativa cultivar XinJiangDaYe / MS.gene031244

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031244.t1	XP_003613438.2	93	57	4	0	18	74	28	84	1.60E-20	108.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031244.t1	O04539	83.0	47	8	0	27	73	8	54	5.1e-15	81.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031244.t1	G7K6W4	93.0	57	4	0	18	74	28	84	1.2e-20	108.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene031244.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031244.t1	MTR_5g036670	92.982	57	4	0	18	74	28	84	2.47e-30	111
MS.gene031244.t1	MTR_8g062700	62.687	67	24	1	9	74	17	83	6.41e-21	84.7
MS.gene031244.t1	MTR_8g062700	62.687	67	24	1	9	74	17	83	6.41e-21	84.7
MS.gene031244.t1	MTR_6g008970	72.917	48	13	0	26	73	56	103	1.28e-17	75.5
MS.gene031244.t1	MTR_6g008995	65.957	47	16	0	27	73	31	77	4.77e-14	65.1
MS.gene031244.t1	MTR_1g105955	62.500	48	18	0	26	73	9	56	7.55e-13	60.1
MS.gene031244.t1	MTR_8g062350	50.980	51	25	0	23	73	4	54	3.53e-12	58.9
MS.gene031244.t1	MTR_1g104940	61.905	42	16	0	33	74	14	55	5.12e-12	57.0
MS.gene031244.t1	MTR_1g105960	62.500	40	15	0	35	74	3	42	5.78e-12	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031244.t1	AT1G70060	81.250	48	9	0	26	73	7	54	4.11e-20	82.4
MS.gene031244.t1	AT1G70060	81.250	48	9	0	26	73	7	54	4.11e-20	82.4
MS.gene031244.t1	AT1G24190	75.000	48	12	0	26	73	7	54	2.48e-18	77.4
MS.gene031244.t1	AT1G24190	75.000	48	12	0	26	73	7	54	2.90e-18	77.4
MS.gene031244.t1	AT1G24190	75.000	48	12	0	26	73	7	54	2.90e-18	77.4
MS.gene031244.t1	AT3G01320	70.000	50	15	0	24	73	48	97	4.33e-18	76.6
MS.gene031244.t1	AT3G01320	70.000	50	15	0	24	73	48	97	4.41e-18	76.6
MS.gene031244.t1	AT1G59890	57.971	69	25	1	9	73	17	85	1.28e-16	72.8
MS.gene031244.t1	AT1G59890	57.971	69	25	1	9	73	17	85	1.28e-16	72.8
MS.gene031244.t1	AT1G59890	57.971	69	25	1	9	73	17	85	1.31e-16	72.8
MS.gene031244.t1	AT1G59890	57.971	69	25	1	9	73	17	85	1.35e-16	72.4
MS.gene031244.t1	AT5G15020	68.085	47	15	0	27	73	46	92	6.76e-16	70.5
MS.gene031244.t1	AT5G15020	68.085	47	15	0	27	73	46	92	7.16e-16	70.5
MS.gene031244.t1	AT1G10450	72.727	44	12	0	31	74	82	125	1.01e-15	70.1
MS.gene031244.t1	AT1G10450	72.727	44	12	0	31	74	31	74	1.10e-15	70.1
MS.gene031244.t1	AT1G10450	72.727	44	12	0	31	74	31	74	1.10e-15	70.1
MS.gene031244.t1	AT5G15025	54.237	59	26	1	16	73	29	87	1.47e-14	63.5
MS.gene031244.t1	AT1G24200	54.167	48	22	0	26	73	7	54	1.82e-12	59.7

Find 12 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTCGATTCGGCGGTTGCC+CGG	0.418989	3.4:+95399361	None:intergenic
TAAGATCAAGTTTCAAGGTC+CGG	0.429714	3.4:-95399380	MS.gene031244:intron
TTAATCAAACCTGAGATTGT+TGG	0.433476	3.4:+95399509	None:intergenic
TTCTCTAAGATCAAGTTTCA+AGG	0.445375	3.4:-95399385	MS.gene031244:intron
ATTAACGTGCCAACAATCTC+AGG	0.477289	3.4:-95399518	MS.gene031244:intron
AAGATCAAGTTTCAAGGTCC+GGG	0.505302	3.4:-95399379	MS.gene031244:CDS
AGACAAGAAGGAAAAGTATG+AGG	0.539440	3.4:-95399276	MS.gene031244:CDS
AGCATATCTCAAGGCGGTCA+AGG	0.594436	3.4:-95399309	MS.gene031244:CDS
TGATGTTGTAGCATATCTCA+AGG	0.606029	3.4:-95399318	MS.gene031244:CDS
GGGCAACCGCCGAATCGAGA+TGG	0.613865	3.4:-95399359	MS.gene031244:CDS
ATTTGTAGAGTTGATACTGA+TGG	0.633224	3.4:-95399127	MS.gene031244:intron
TGTTGTAGCATATCTCAAGG+CGG	0.799861	3.4:-95399315	MS.gene031244:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTTGTTTTTGTATATATATT+GGG	-	chr3.4:95399445-95399464	MS.gene031244:intron	15.0%
!!	ATATATATACAAAAACAACG+TGG	+	chr3.4:95399444-95399463	None:intergenic	20.0%
!!!	AATTGATTTGTTTATCCTTT+TGG	-	chr3.4:95399494-95399513	MS.gene031244:intron	20.0%
!!!	CGTTGTTTTTGTATATATAT+TGG	-	chr3.4:95399444-95399463	MS.gene031244:intron	20.0%
!	TTTGTATATATATTGGGAGA+GGG	-	chr3.4:95399451-95399470	MS.gene031244:intron	25.0%
!!	TTTTGTATATATATTGGGAG+AGG	-	chr3.4:95399450-95399469	MS.gene031244:intron	25.0%
!!!	TGAAAGATTTTAAAGCACAA+AGG	-	chr3.4:95399409-95399428	MS.gene031244:intron	25.0%
	TCATTTCTTTATTGCTCCTT+CGG	+	chr3.4:95399186-95399205	None:intergenic	30.0%
	TTAATCAAACCTGAGATTGT+TGG	+	chr3.4:95399141-95399160	None:intergenic	30.0%
	TTGAGTGTGATCTTATCATA+TGG	+	chr3.4:95399225-95399244	None:intergenic	30.0%
!!	ATTTGTAGAGTTGATACTGA+TGG	-	chr3.4:95399520-95399539	MS.gene031244:CDS	30.0%
!!	TTCTCTAAGATCAAGTTTCA+AGG	-	chr3.4:95399262-95399281	MS.gene031244:CDS	30.0%
!!!	GGATATTTTTCAAGACAAGA+AGG	-	chr3.4:95399359-95399378	MS.gene031244:CDS	30.0%
	AACTCTACAAATGCACCAAA+AGG	+	chr3.4:95399512-95399531	None:intergenic	35.0%
	TAAGATCAAGTTTCAAGGTC+CGG	-	chr3.4:95399267-95399286	MS.gene031244:CDS	35.0%
	TGATGTTGTAGCATATCTCA+AGG	-	chr3.4:95399329-95399348	MS.gene031244:CDS	35.0%
!	AAAGTATGAGGACTTTTTGG+AGG	-	chr3.4:95399383-95399402	MS.gene031244:intron	35.0%
!	AGACAAGAAGGAAAAGTATG+AGG	-	chr3.4:95399371-95399390	MS.gene031244:CDS	35.0%
!	AGTTTTTGACCATCTCGATT+CGG	+	chr3.4:95399300-95399319	None:intergenic	35.0%
!!	GGAAAAGTATGAGGACTTTT+TGG	-	chr3.4:95399380-95399399	MS.gene031244:intron	35.0%
	AAGATCAAGTTTCAAGGTCC+GGG	-	chr3.4:95399268-95399287	MS.gene031244:CDS	40.0%
	ATTAACGTGCCAACAATCTC+AGG	-	chr3.4:95399129-95399148	MS.gene031244:intron	40.0%
	GATCACACTCAACATGATTG+TGG	-	chr3.4:95399233-95399252	MS.gene031244:intron	40.0%
	TGTTGTAGCATATCTCAAGG+CGG	-	chr3.4:95399332-95399351	MS.gene031244:CDS	40.0%
	TTTATTGCTCCTTCGGCTCT+AGG	+	chr3.4:95399179-95399198	None:intergenic	45.0%
!!!	TTTTGACCATCTCGATTCGG+CGG	+	chr3.4:95399297-95399316	None:intergenic	45.0%
	AGCATATCTCAAGGCGGTCA+AGG	-	chr3.4:95399338-95399357	MS.gene031244:CDS	50.0%
	TACTTAGTGCCTAGAGCCGA+AGG	-	chr3.4:95399167-95399186	MS.gene031244:intron	50.0%
!!	ATCTCGATTCGGCGGTTGCC+CGG	+	chr3.4:95399289-95399308	None:intergenic	60.0%
	GGGCAACCGCCGAATCGAGA+TGG	-	chr3.4:95399288-95399307	MS.gene031244:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	95399114	95399555	95399114	ID=MS.gene031244
chr3.4	mRNA	95399114	95399555	95399114	ID=MS.gene031244.t1;Parent=MS.gene031244
chr3.4	exon	95399519	95399555	95399519	ID=MS.gene031244.t1.exon1;Parent=MS.gene031244.t1
chr3.4	CDS	95399519	95399555	95399519	ID=cds.MS.gene031244.t1;Parent=MS.gene031244.t1
chr3.4	exon	95399239	95399398	95399239	ID=MS.gene031244.t1.exon2;Parent=MS.gene031244.t1
chr3.4	CDS	95399239	95399398	95399239	ID=cds.MS.gene031244.t1;Parent=MS.gene031244.t1
chr3.4	exon	95399114	95399141	95399114	ID=MS.gene031244.t1.exon3;Parent=MS.gene031244.t1
chr3.4	CDS	95399114	95399141	95399114	ID=cds.MS.gene031244.t1;Parent=MS.gene031244.t1

Gene Sequence

>MS.gene031244

ATGAATTGTGAAGACATTAACGTGCCAACAATCTCAGGTTTGATTAAGATAAATACTTAGTGCCTAGAGCCGAAGGAGCAATAAAGAAATGAAAACATAATTCAGAAACCATATGATAAGATCACACTCAACATGATTGTGGTGTATATTCTCTAAGATCAAGTTTCAAGGTCCGGGCAACCGCCGAATCGAGATGGTCAAAAACTGACTACTAATGATGTTGTAGCATATCTCAAGGCGGTCAAGGATATTTTTCAAGACAAGAAGGAAAAGTATGAGGACTTTTTGGAGGTTATGAAAGATTTTAAAGCACAAAGGTATTTTGTACCACGTTGTTTTTGTATATATATTGGGAGAGGGATTATGAATTTGTTAGTTGTAATTGATTTGTTTATCCTTTTGGTGCATTTGTAGAGTTGATACTGATGGTGTCATTGAATGA

Protein sequence

>MS.gene031244.t1

MNCEDINVPTISDQVSRSGQPPNRDGQKLTTNDVVAYLKAVKDIFQDKKEKYEDFLEVMKDFKAQRVDTDGVIE