AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031301

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031301.t1 MTR_2g082090 99.136 579 5 0 1 579 1 579 0.0 1193
MS.gene031301.t1 MTR_5g097480 64.341 387 135 1 195 578 157 543 0.0 529
MS.gene031301.t1 MTR_3g056110 58.871 372 153 0 207 578 170 541 4.32e-169 491
MS.gene031301.t1 MTR_5g094450 49.505 505 209 6 79 578 68 531 1.62e-166 484
MS.gene031301.t1 MTR_4g133660 48.936 470 187 3 117 578 118 542 3.71e-158 463
MS.gene031301.t1 MTR_1g029420 53.316 377 175 1 202 578 216 591 2.88e-147 437
MS.gene031301.t1 MTR_3g089055 53.243 370 173 0 210 579 201 570 9.01e-144 427
MS.gene031301.t1 MTR_3g089055 53.243 370 173 0 210 579 196 565 1.20e-143 427
MS.gene031301.t1 MTR_2g026250 53.439 378 169 2 205 578 221 595 8.77e-143 426
MS.gene031301.t1 MTR_7g027190 34.592 503 274 9 113 578 188 672 3.41e-79 263
MS.gene031301.t1 MTR_4g064150 33.245 376 246 3 208 578 357 732 3.09e-69 238
MS.gene031301.t1 MTR_4g064160 32.258 372 251 1 208 578 312 683 1.33e-67 232
MS.gene031301.t1 MTR_2g097410 33.245 376 244 4 208 578 367 740 1.69e-66 230
MS.gene031301.t1 MTR_7g057230 36.533 375 222 6 210 578 292 656 7.58e-66 227
MS.gene031301.t1 MTR_4g064180 33.333 375 245 3 208 577 250 624 4.75e-65 224
MS.gene031301.t1 MTR_4g064120 29.401 551 352 11 40 577 98 624 6.27e-65 224
MS.gene031301.t1 MTR_8g442410 36.154 390 216 9 208 579 151 525 5.84e-64 219
MS.gene031301.t1 MTR_6g047750 36.623 385 214 10 210 579 293 662 1.53e-62 218
MS.gene031301.t1 MTR_4g104020 32.115 383 238 5 209 578 146 519 1.68e-62 214
MS.gene031301.t1 MTR_3g065980 32.373 451 251 11 148 578 123 539 2.26e-62 215
MS.gene031301.t1 MTR_4g097080 32.105 380 239 6 210 578 131 502 1.89e-60 209
MS.gene031301.t1 MTR_7g062120 31.452 372 254 1 208 578 368 739 1.93e-60 214
MS.gene031301.t1 MTR_7g074650 35.695 367 215 7 216 578 442 791 2.33e-60 214
MS.gene031301.t1 MTR_4g064200 32.440 373 247 4 208 578 280 649 9.81e-60 210
MS.gene031301.t1 MTR_2g097390 32.095 377 243 4 208 578 271 640 4.50e-58 205
MS.gene031301.t1 MTR_2g097350 32.095 377 243 7 208 578 270 639 1.39e-57 204
MS.gene031301.t1 MTR_2g097310 30.749 374 252 3 208 578 268 637 2.35e-57 203
MS.gene031301.t1 MTR_2g097473 31.807 415 270 7 166 578 250 653 3.15e-56 201
MS.gene031301.t1 MTR_3g022830 30.935 417 230 7 213 578 26 435 3.41e-56 196
MS.gene031301.t1 MTR_2g097467 31.688 385 259 3 195 578 272 653 8.45e-56 199
MS.gene031301.t1 MTR_4g076140 31.395 430 229 8 209 578 45 468 1.01e-55 195
MS.gene031301.t1 MTR_2g097463 30.935 417 280 4 163 578 245 654 1.31e-54 196
MS.gene031301.t1 MTR_4g077760 37.330 367 202 12 225 578 203 554 9.86e-54 192
MS.gene031301.t1 MTR_5g015490 31.937 382 239 8 212 578 114 489 1.07e-51 185
MS.gene031301.t1 MTR_4g102790 32.031 384 238 11 208 578 288 661 5.67e-50 184
MS.gene031301.t1 MTR_4g076020 33.062 369 215 9 216 579 88 429 5.91e-49 176
MS.gene031301.t1 MTR_1g069725 30.787 432 261 14 162 578 54 462 2.29e-48 175
MS.gene031301.t1 MTR_2g097380 26.767 467 280 9 133 578 135 560 4.32e-43 162
MS.gene031301.t1 MTR_1g096030 29.032 465 256 17 162 579 22 459 3.46e-42 158
MS.gene031301.t1 MTR_3g072710 31.818 396 231 13 209 578 117 499 2.67e-41 156
MS.gene031301.t1 MTR_2g099110 29.124 388 245 8 211 578 73 450 5.70e-41 154
MS.gene031301.t1 MTR_5g058860 30.928 388 243 10 209 578 116 496 4.18e-40 153
MS.gene031301.t1 MTR_2g089100 25.373 402 260 7 209 578 49 442 6.95e-38 146
MS.gene031301.t1 MTR_5g015950 25.527 427 248 11 209 578 32 445 3.90e-33 132
MS.gene031301.t1 MTR_3g053270 25.527 427 248 11 209 578 32 445 3.90e-33 132
MS.gene031301.t1 MTR_5g009080 35.238 210 129 2 209 417 53 256 8.18e-32 129
MS.gene031301.t1 MTR_5g009080 42.282 149 85 1 431 578 329 477 1.25e-29 122
MS.gene031301.t1 MTR_2g034250 25.831 391 256 10 209 578 207 584 1.02e-31 130
MS.gene031301.t1 MTR_4g095500 24.153 472 293 10 166 578 2 467 1.70e-30 125
MS.gene031301.t1 MTR_2g034260 25.781 384 264 9 209 578 207 583 9.98e-29 121
MS.gene031301.t1 MTR_2g034280 25.131 382 267 9 209 578 200 574 1.70e-27 117
MS.gene031301.t1 MTR_8g093070 27.543 403 246 17 190 578 125 495 8.26e-27 114
MS.gene031301.t1 MTR_0092s0100 26.042 384 268 8 197 578 358 727 2.10e-26 114
MS.gene031301.t1 MTR_0092s0100 26.042 384 268 8 197 578 361 730 2.15e-26 114
MS.gene031301.t1 MTR_7g109580 24.091 440 291 13 151 578 145 553 2.95e-26 113
MS.gene031301.t1 MTR_4g026485 26.163 344 226 10 243 578 300 623 1.94e-25 111
MS.gene031301.t1 MTR_1g086970 25.424 354 231 12 243 578 139 477 6.04e-24 105
MS.gene031301.t1 MTR_3g022580 39.450 109 66 0 470 578 74 182 1.40e-17 81.3
MS.gene031301.t1 MTR_3g022005 42.391 92 53 0 487 578 33 124 9.05e-15 71.6
MS.gene031301.t1 MTR_8g077940 22.635 296 203 10 292 578 262 540 5.65e-13 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031301.t1 AT1G50600 63.061 379 140 0 200 578 147 525 2.42e-176 509
MS.gene031301.t1 AT1G50600 63.061 379 140 0 200 578 218 596 9.92e-176 510
MS.gene031301.t1 AT1G50600 63.061 379 140 0 200 578 222 600 1.28e-175 510
MS.gene031301.t1 AT5G48150 61.942 381 144 1 199 578 109 489 1.23e-170 493
MS.gene031301.t1 AT5G48150 61.942 381 144 1 199 578 109 489 1.23e-170 493
MS.gene031301.t1 AT5G48150 61.942 381 144 1 199 578 109 489 1.23e-170 493
MS.gene031301.t1 AT5G48150 61.942 381 144 1 199 578 109 489 1.23e-170 493
MS.gene031301.t1 AT2G04890 60.317 378 143 1 201 578 42 412 1.07e-164 475
MS.gene031301.t1 AT4G17230 54.011 374 172 0 205 578 151 524 1.83e-144 428
MS.gene031301.t1 AT4G17230 54.011 374 172 0 205 578 151 524 1.83e-144 428
MS.gene031301.t1 AT1G21450 46.039 467 227 7 114 579 151 593 5.69e-138 414
MS.gene031301.t1 AT1G07530 33.333 375 248 2 206 578 390 764 2.15e-69 239
MS.gene031301.t1 AT5G52510 36.104 385 221 6 210 579 266 640 6.24e-67 229
MS.gene031301.t1 AT2G29060 33.600 375 245 3 208 578 280 654 3.93e-65 225
MS.gene031301.t1 AT2G29060 33.600 375 245 3 208 578 317 691 6.12e-65 225
MS.gene031301.t1 AT3G03450 34.759 374 231 7 209 578 180 544 1.80e-64 221
MS.gene031301.t1 AT1G66350 33.654 416 246 11 169 578 114 505 6.22e-64 218
MS.gene031301.t1 AT5G17490 33.690 374 229 8 209 578 157 515 3.71e-62 214
MS.gene031301.t1 AT3G46600 34.140 372 241 4 209 578 1 370 1.11e-61 208
MS.gene031301.t1 AT3G46600 34.316 373 241 4 208 578 176 546 1.71e-61 213
MS.gene031301.t1 AT3G46600 34.316 373 241 4 208 578 208 578 2.09e-61 213
MS.gene031301.t1 AT5G59450 32.626 377 246 4 208 578 223 597 9.85e-61 212
MS.gene031301.t1 AT3G54220 34.625 413 249 9 172 578 252 649 1.75e-60 212
MS.gene031301.t1 AT1G50420 31.323 431 229 5 209 578 54 478 7.24e-60 207
MS.gene031301.t1 AT1G14920 35.279 377 220 10 209 578 169 528 8.21e-60 208
MS.gene031301.t1 AT2G01570 33.598 378 225 9 209 578 221 580 2.87e-57 202
MS.gene031301.t1 AT2G37650 31.452 372 252 2 208 578 331 700 2.92e-57 204
MS.gene031301.t1 AT2G37650 31.452 372 252 2 208 578 343 712 3.60e-57 204
MS.gene031301.t1 AT1G07520 31.316 380 251 6 208 578 150 528 9.46e-57 199
MS.gene031301.t1 AT1G07520 31.316 380 251 6 208 578 150 528 9.46e-57 199
MS.gene031301.t1 AT1G07520 31.316 380 251 6 208 578 314 692 3.46e-56 201
MS.gene031301.t1 AT1G07520 31.316 380 251 6 208 578 315 693 3.72e-56 201
MS.gene031301.t1 AT5G66770 34.383 381 227 10 209 579 217 584 1.17e-53 192
MS.gene031301.t1 AT2G29065 30.971 381 252 6 208 578 248 627 2.66e-52 189
MS.gene031301.t1 AT3G50650 34.726 383 225 10 208 578 172 541 9.72e-52 186
MS.gene031301.t1 AT1G55580 34.304 309 179 6 285 578 145 444 1.31e-49 178
MS.gene031301.t1 AT5G41920 31.486 397 246 8 191 578 20 399 1.15e-47 172
MS.gene031301.t1 AT4G37650 30.102 392 240 10 212 578 146 528 1.83e-47 174
MS.gene031301.t1 AT1G63100 31.877 389 239 8 208 578 273 653 8.10e-47 174
MS.gene031301.t1 AT1G63100 31.877 389 239 8 208 578 248 628 1.07e-46 174
MS.gene031301.t1 AT4G08250 28.198 383 252 10 209 578 104 476 3.98e-34 135
MS.gene031301.t1 AT3G49950 24.031 387 271 9 209 578 27 407 1.46e-29 121
MS.gene031301.t1 AT2G45160 26.171 363 218 12 237 578 305 638 9.27e-24 106
MS.gene031301.t1 AT3G60630 27.723 303 188 8 291 578 335 621 6.80e-23 103
MS.gene031301.t1 AT3G13840 25.109 458 301 14 135 578 80 509 2.04e-20 95.1
MS.gene031301.t1 AT5G67411 34.821 112 71 2 405 516 89 198 2.03e-11 63.9

Find 153 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGTCCCTACTTAAAATT+TGG 0.130718 2.1:+18578102 MS.gene031301:CDS
AAAGATGGGGCAATGCTCTT+AGG 0.253125 2.1:+18578903 MS.gene031301:CDS
CTTCAGGCTCTCTGCACTTA+AGG 0.306715 2.1:-18578041 None:intergenic
CCAGAGGCTAGGTGCTTATA+TGG 0.309502 2.1:+18577966 MS.gene031301:CDS
TCCTTGATGTCTAAGAAATT+CGG 0.327588 2.1:+18578345 MS.gene031301:CDS
GTGAGTAATTCTTCGCCTTC+AGG 0.332767 2.1:-18578057 None:intergenic
TTCATTGGGTGAGAGCATTA+AGG 0.336043 2.1:+18577651 MS.gene031301:CDS
AGAATGATCTTTGCGTATAA+TGG 0.338653 2.1:-18577480 None:intergenic
TACCACATCTAATTCTTCAT+TGG 0.339637 2.1:+18577636 MS.gene031301:CDS
TCCACAAGGGTGACCACTTT+AGG 0.340706 2.1:-18578552 None:intergenic
GAGCCATTGCTGAAGCTTGC+AGG 0.347306 2.1:+18578142 MS.gene031301:CDS
GGCATCCCGGTTGAGTTTCA+TGG 0.352118 2.1:+18578366 MS.gene031301:CDS
TTCAAGTGATGAGTTGTAAG+AGG 0.354731 2.1:-18577452 None:intergenic
GGATCCTTGAAAGTTGGCTT+TGG 0.385729 2.1:+18577319 MS.gene031301:CDS
TAATGTGGAGCAGCATTGTT+TGG 0.392234 2.1:+18578695 MS.gene031301:CDS
GTTTCCAAAGCCAACTTTCA+AGG 0.393842 2.1:-18577323 None:intergenic
TTCTAGCCGTTTCTATCAAT+CGG 0.394967 2.1:-18577915 None:intergenic
CTACTTAAAATTTGGTTACA+TGG 0.400326 2.1:+18578110 MS.gene031301:CDS
AGCATGTCTCTTGTCACATC+TGG 0.401716 2.1:-18578405 None:intergenic
ACACAACACCTTTCTTCAAC+AGG 0.402167 2.1:+18578592 MS.gene031301:CDS
ACCACATCTAATTCTTCATT+GGG 0.403891 2.1:+18577637 MS.gene031301:CDS
AGCTTCAGCAATGGCTCCAT+TGG 0.408396 2.1:-18578136 None:intergenic
CAAGCTCTTGCCGCAAGACC+TGG 0.414231 2.1:+18578231 MS.gene031301:CDS
GTTGCAAGGACGGAAGCATC+AGG 0.425786 2.1:+18578000 MS.gene031301:CDS
GGAATCCCATGAAACTCAAC+CGG 0.428270 2.1:-18578372 None:intergenic
GTCACATCTGGACCGAAAAC+AGG 0.432073 2.1:-18578393 None:intergenic
TATCAGACACGGAGAAGCTT+TGG 0.432770 2.1:+18578431 MS.gene031301:CDS
GAAAATCCGACACGCCTTGT+TGG 0.433986 2.1:+18577573 MS.gene031301:CDS
TTCAGGCTCTCTGCACTTAA+GGG 0.435350 2.1:-18578040 None:intergenic
CGCACGTGGGGTGCCCCGCC+AGG 0.442006 2.1:-18578249 None:intergenic
ATTTGATTGCCTGCTACTGC+TGG 0.442739 2.1:-18577758 None:intergenic
CTCCTTGCTATTTCTTGAGA+GGG 0.443191 2.1:-18578667 None:intergenic
ATGAGAGGAAAACGGTGAAT+CGG 0.450658 2.1:-18577350 None:intergenic
TTGCTCCATGATCTATATCT+CGG 0.451721 2.1:-18577688 None:intergenic
AGCAAACCATTTGGCAGTGA+TGG 0.458184 2.1:-18577295 None:intergenic
GAAATGCTGTGTCTATCAAT+GGG 0.460974 2.1:+18577935 MS.gene031301:CDS
GTGGTAACTTCGTTATCATC+GGG 0.461161 2.1:-18577619 None:intergenic
TTGCTTGGATCCTTGAAAGT+TGG 0.461484 2.1:+18577313 MS.gene031301:CDS
AGCCACGAGCAACACAATTC+AGG 0.463613 2.1:+18577406 MS.gene031301:CDS
AAGGATCCAAGCAAACCATT+TGG 0.464280 2.1:-18577304 None:intergenic
TCACTGCCAAATGGTTTGCT+TGG 0.464474 2.1:+18577298 MS.gene031301:CDS
AGAAATGCTGTGTCTATCAA+TGG 0.466776 2.1:+18577934 MS.gene031301:CDS
AAGATGGGGCAATGCTCTTA+GGG 0.467770 2.1:+18578904 MS.gene031301:CDS
TGAGAGGAAAACGGTGAATC+GGG 0.469261 2.1:-18577349 None:intergenic
CCATATAAGCACCTAGCCTC+TGG 0.472152 2.1:-18577966 None:intergenic
TGTAGAGAAACGGCGGAAGT+TGG 0.473337 2.1:+18577792 MS.gene031301:CDS
AAGTCTAGGTTGACAATGGC+TGG 0.474170 2.1:+18578792 MS.gene031301:CDS
CTCTAAATATGCCCGTGGCA+AGG 0.478020 2.1:+18578296 MS.gene031301:CDS
TCTATGAACCTGTTGAAGAA+AGG 0.480955 2.1:-18578600 None:intergenic
CTAGGTGCTTATATGGTAGA+AGG 0.487203 2.1:+18577973 MS.gene031301:CDS
GCATCCCGGTTGAGTTTCAT+GGG 0.488489 2.1:+18578367 MS.gene031301:CDS
TCCTAAAGTGGTCACCCTTG+TGG 0.490892 2.1:+18578551 MS.gene031301:CDS
TATACACTAGTTGAGAAAGA+TGG 0.493554 2.1:+18578888 MS.gene031301:CDS
GCACCCCACGTGCGGATCAC+AGG 0.501241 2.1:+18578258 MS.gene031301:CDS
AGTTACAGTATGAGTAATGC+AGG 0.503062 2.1:+18577250 MS.gene031301:CDS
TCTAAATATGCCCGTGGCAA+GGG 0.504773 2.1:+18578297 MS.gene031301:CDS
GCCGAATTTCTTAGACATCA+AGG 0.506271 2.1:-18578346 None:intergenic
TGCTCCATGATCTATATCTC+GGG 0.508139 2.1:-18577687 None:intergenic
TGTCATTGCATGCGAAGGTA+AGG 0.508199 2.1:+18578734 MS.gene031301:CDS
GGAGCAGCATTGTTTGGCTC+GGG 0.508648 2.1:+18578701 MS.gene031301:CDS
TGGAGCAGCATTGTTTGGCT+CGG 0.508972 2.1:+18578700 MS.gene031301:CDS
ATACACTAGTTGAGAAAGAT+GGG 0.509202 2.1:+18578889 MS.gene031301:CDS
TTTGAACATATTGCGACCCT+TGG 0.510077 2.1:-18577717 None:intergenic
GTCTAAGAAATTCGGCATCC+CGG 0.511547 2.1:+18578353 MS.gene031301:CDS
ATCTCTACAAGGGTTTCCAT+CGG 0.514255 2.1:+18577825 MS.gene031301:CDS
TCTTGCCGCAAGACCTGGCG+GGG 0.514298 2.1:+18578236 MS.gene031301:CDS
GATGGAAAAGATGACATGTA+AGG 0.515221 2.1:-18577277 None:intergenic
TCTCCTTGCTATTTCTTGAG+AGG 0.515388 2.1:-18578668 None:intergenic
TGATCTTTGCGTATAATGGT+TGG 0.518085 2.1:-18577476 None:intergenic
TGTGGTAACTTCGTTATCAT+CGG 0.521741 2.1:-18577620 None:intergenic
CACTCTCATCAGCTGTGTGA+TGG 0.522559 2.1:-18578476 None:intergenic
CCACGGGCATATTTAGAGAC+CGG 0.528939 2.1:-18578291 None:intergenic
CTCTTGCCGCAAGACCTGGC+GGG 0.529456 2.1:+18578235 MS.gene031301:CDS
TCTCTACAAGGGTTTCCATC+GGG 0.530378 2.1:+18577826 MS.gene031301:CDS
CCGGCCCGAGATATAGATCA+TGG 0.534861 2.1:+18577683 MS.gene031301:CDS
AATTCAGGAGAAATCCTCTC+TGG 0.536357 2.1:+18577421 MS.gene031301:CDS
GGGTGCCCCGCCAGGTCTTG+CGG 0.536838 2.1:-18578241 None:intergenic
TGCTGAAGCTTGCAGGAATG+AGG 0.537831 2.1:+18578149 MS.gene031301:CDS
ATTTGATATCTGCTTCAGCT+TGG 0.537926 2.1:+18578940 MS.gene031301:CDS
CTCCTGAATTGTGTTGCTCG+TGG 0.538853 2.1:-18577408 None:intergenic
GTTGAGCGACACGAACTACT+TGG 0.539879 2.1:+18578762 MS.gene031301:CDS
GAATCAAAATGAGAGGAAAA+CGG 0.543811 2.1:-18577358 None:intergenic
TTTGGTTACATGGCTGCCAA+TGG 0.544829 2.1:+18578120 MS.gene031301:CDS
TACTTCTAGTCCCTTGCCAC+GGG 0.544995 2.1:-18578307 None:intergenic
AGCATTAAGGAAACAGCATC+CGG 0.546690 2.1:+18577664 MS.gene031301:CDS
GGCAAGGGACTAGAAGTAGT+TGG 0.546746 2.1:+18578312 MS.gene031301:CDS
AGAAGGTCTTGTTGCAAGGA+CGG 0.548822 2.1:+18577990 MS.gene031301:CDS
AGATTGCTCAAGGAACTCAA+TGG 0.549437 2.1:+18578196 MS.gene031301:CDS
TGTTTGATTCTTGCTCCACA+AGG 0.549494 2.1:-18578566 None:intergenic
GCTCTTGCCGCAAGACCTGG+CGG 0.550382 2.1:+18578234 MS.gene031301:CDS
ATAGACTTTCAGATTGCTCA+AGG 0.550384 2.1:+18578186 MS.gene031301:CDS
GATCACAGGAATCGATGATC+CGG 0.550832 2.1:+18578272 MS.gene031301:CDS
AATCTCAAAAGTCCATCCCT+TGG 0.551200 2.1:-18578513 None:intergenic
CGATTCCTGTGATCCGCACG+TGG 0.552219 2.1:-18578263 None:intergenic
CACATGTTACCAGCAGTAGC+AGG 0.553147 2.1:+18577749 MS.gene031301:CDS
TAAGCACCTAGCCTCTGGAT+TGG 0.558693 2.1:-18577961 None:intergenic
AGAAACTTTAGATTACTACT+TGG 0.559206 2.1:+18578620 MS.gene031301:CDS
GAAGTCTCTCTCTCCTAAAG+TGG 0.559272 2.1:+18578539 MS.gene031301:CDS
TCAAGTGATGAGTTGTAAGA+GGG 0.561646 2.1:-18577451 None:intergenic
CAGACACGGAGAAGCTTTGG+CGG 0.563257 2.1:+18578434 MS.gene031301:CDS
AAGCAGTGCATGTAGAGAAA+CGG 0.563714 2.1:+18577782 MS.gene031301:CDS
GAAGAGGATTCATCTCTACA+AGG 0.568777 2.1:+18577814 MS.gene031301:CDS
TACTTGGTAAGTGGAAGTCT+AGG 0.570428 2.1:+18578778 MS.gene031301:CDS
GGAACTAGAAACTGCTCTGA+TGG 0.573852 2.1:+18577594 MS.gene031301:CDS
GTCAATGTCATTGCATGCGA+AGG 0.575792 2.1:+18578729 MS.gene031301:CDS
GAAACGGCGGAAGTTGGAAG+AGG 0.580531 2.1:+18577798 MS.gene031301:CDS
GACACGAACTACTTGGTAAG+TGG 0.581297 2.1:+18578769 MS.gene031301:CDS
CCATGATCTATATCTCGGGC+CGG 0.582570 2.1:-18577683 None:intergenic
TCTTGAGAGGGTGACATCGA+TGG 0.582838 2.1:-18578655 None:intergenic
TGCATGCGAAGGTAAGGAAA+GGG 0.588907 2.1:+18578740 MS.gene031301:CDS
TGGTAGAAGGTCTTGTTGCA+AGG 0.589192 2.1:+18577986 MS.gene031301:CDS
GGGGAACCAATCCAGAGGCT+AGG 0.596146 2.1:+18577955 MS.gene031301:CDS
CTACTTCTAGTCCCTTGCCA+CGG 0.597233 2.1:-18578308 None:intergenic
CACCCTCTCAAGAAATAGCA+AGG 0.600143 2.1:+18578665 MS.gene031301:CDS
TTGCATGCGAAGGTAAGGAA+AGG 0.601660 2.1:+18578739 MS.gene031301:CDS
CTAGTTCCAACAAGGCGTGT+CGG 0.604341 2.1:-18577579 None:intergenic
CTGGCGGGGCACCCCACGTG+CGG 0.605255 2.1:+18578250 MS.gene031301:CDS
GTGTTGATGTGAATAATCCA+AGG 0.605633 2.1:+18578496 MS.gene031301:CDS
CATGGAGCAATGAGCACCAA+GGG 0.610609 2.1:+18577701 MS.gene031301:CDS
AGTATCAGAATCAAAATGAG+AGG 0.612504 2.1:-18577365 None:intergenic
CTACTGCTGGTAACATGTGA+CGG 0.612714 2.1:-18577745 None:intergenic
AAGAGGATTCATCTCTACAA+GGG 0.614893 2.1:+18577815 MS.gene031301:CDS
ATTCCTGCAAGCTTCAGCAA+TGG 0.622249 2.1:-18578145 None:intergenic
ATTCCTGTGATCCGCACGTG+GGG 0.622362 2.1:-18578261 None:intergenic
TAGCAAGGAGAGGATTAATG+TGG 0.632293 2.1:+18578680 MS.gene031301:CDS
GCTCTGAATAGCATCTCAGA+AGG 0.636572 2.1:-18578863 None:intergenic
GATTCCTGTGATCCGCACGT+GGG 0.637104 2.1:-18578262 None:intergenic
TTGCAAGGACGGAAGCATCA+GGG 0.638897 2.1:+18578001 MS.gene031301:CDS
GTTTGATTCTTGCTCCACAA+GGG 0.642203 2.1:-18578565 None:intergenic
AATTGCTTCAAATCGCCCGA+TGG 0.642596 2.1:-18577841 None:intergenic
ATGGGGCAATGCTCTTAGGG+TGG 0.644351 2.1:+18578907 MS.gene031301:CDS
CTTAAGTGCAGAGAGCCTGA+AGG 0.646181 2.1:+18578042 MS.gene031301:CDS
ACCCAATGAAGAATTAGATG+TGG 0.646339 2.1:-18577638 None:intergenic
GATTGCATGTGCCAAAGCCA+TGG 0.652232 2.1:+18577867 MS.gene031301:CDS
AGCAGTTTCTAGTTCCAACA+AGG 0.652329 2.1:-18577587 None:intergenic
CCGGTCTCTAAATATGCCCG+TGG 0.655502 2.1:+18578291 MS.gene031301:CDS
TGGTAACTTCGTTATCATCG+GGG 0.655578 2.1:-18577618 None:intergenic
GATGTGAATAATCCAAGGGA+TGG 0.658106 2.1:+18578501 MS.gene031301:CDS
GTGGAAGTCTAGGTTGACAA+TGG 0.658484 2.1:+18578788 MS.gene031301:CDS
GCTGTGTGATGGAGTTGCAG+TGG 0.659602 2.1:-18578465 None:intergenic
CAAGTGATGAGTTGTAAGAG+GGG 0.663939 2.1:-18577450 None:intergenic
GAATTCACATAAGAACTCAA+AGG 0.664196 2.1:-18578828 None:intergenic
GAATCCCATGAAACTCAACC+GGG 0.671033 2.1:-18578371 None:intergenic
TCATGGAGCAATGAGCACCA+AGG 0.677430 2.1:+18577700 MS.gene031301:CDS
TCTCAAGAAATAGCAAGGAG+AGG 0.677996 2.1:+18578670 MS.gene031301:CDS
GACATGCTTGATATCAGACA+CGG 0.687158 2.1:+18578420 MS.gene031301:CDS
CAGTGCATGTAGAGAAACGG+CGG 0.688096 2.1:+18577785 MS.gene031301:CDS
GGTAACTTCGTTATCATCGG+GGG 0.691927 2.1:-18577617 None:intergenic
AAGAGGGGCTTATACCAGAG+AGG 0.693678 2.1:-18577435 None:intergenic
TGTTGATGTGAATAATCCAA+GGG 0.710085 2.1:+18578497 MS.gene031301:CDS
AAATGCTGTGTCTATCAATG+GGG 0.714938 2.1:+18577936 MS.gene031301:CDS
TACACTAGTTGAGAAAGATG+GGG 0.719725 2.1:+18578890 MS.gene031301:CDS
TCAATGGGGAACCAATCCAG+AGG 0.733104 2.1:+18577950 MS.gene031301:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAACCAAATTTTAAGTA+GGG - chr2.1:18578111-18578130 None:intergenic 20.0%
! AGAAACTTTAGATTACTACT+TGG + chr2.1:18578620-18578639 MS.gene031301:CDS 25.0%
! CTACTTAAAATTTGGTTACA+TGG + chr2.1:18578110-18578129 MS.gene031301:CDS 25.0%
!!! CATGTAACCAAATTTTAAGT+AGG - chr2.1:18578112-18578131 None:intergenic 25.0%
ACCACATCTAATTCTTCATT+GGG + chr2.1:18577637-18577656 MS.gene031301:CDS 30.0%
AGAATGATCTTTGCGTATAA+TGG - chr2.1:18577483-18577502 None:intergenic 30.0%
AGTATCAGAATCAAAATGAG+AGG - chr2.1:18577368-18577387 None:intergenic 30.0%
ATACACTAGTTGAGAAAGAT+GGG + chr2.1:18578889-18578908 MS.gene031301:CDS 30.0%
ATCTGTCCCTACTTAAAATT+TGG + chr2.1:18578102-18578121 MS.gene031301:CDS 30.0%
GAATCAAAATGAGAGGAAAA+CGG - chr2.1:18577361-18577380 None:intergenic 30.0%
GAATTCACATAAGAACTCAA+AGG - chr2.1:18578831-18578850 None:intergenic 30.0%
TACCACATCTAATTCTTCAT+TGG + chr2.1:18577636-18577655 MS.gene031301:CDS 30.0%
TATACACTAGTTGAGAAAGA+TGG + chr2.1:18578888-18578907 MS.gene031301:CDS 30.0%
TCCTTGATGTCTAAGAAATT+CGG + chr2.1:18578345-18578364 MS.gene031301:CDS 30.0%
TGTTGATGTGAATAATCCAA+GGG + chr2.1:18578497-18578516 MS.gene031301:CDS 30.0%
! TTTTGACCGATTGATAGAAA+CGG + chr2.1:18577909-18577928 MS.gene031301:CDS 30.0%
AAATGCTGTGTCTATCAATG+GGG + chr2.1:18577936-18577955 MS.gene031301:CDS 35.0%
AAGAGGATTCATCTCTACAA+GGG + chr2.1:18577815-18577834 MS.gene031301:CDS 35.0%
ACCCAATGAAGAATTAGATG+TGG - chr2.1:18577641-18577660 None:intergenic 35.0%
AGAAATGCTGTGTCTATCAA+TGG + chr2.1:18577934-18577953 MS.gene031301:CDS 35.0%
AGTTACAGTATGAGTAATGC+AGG + chr2.1:18577250-18577269 MS.gene031301:CDS 35.0%
ATAGACTTTCAGATTGCTCA+AGG + chr2.1:18578186-18578205 MS.gene031301:CDS 35.0%
ATTTGATATCTGCTTCAGCT+TGG + chr2.1:18578940-18578959 MS.gene031301:CDS 35.0%
GAAATGCTGTGTCTATCAAT+GGG + chr2.1:18577935-18577954 MS.gene031301:CDS 35.0%
GATGGAAAAGATGACATGTA+AGG - chr2.1:18577280-18577299 None:intergenic 35.0%
TACACTAGTTGAGAAAGATG+GGG + chr2.1:18578890-18578909 MS.gene031301:CDS 35.0%
TCAAGTGATGAGTTGTAAGA+GGG - chr2.1:18577454-18577473 None:intergenic 35.0%
TCTATGAACCTGTTGAAGAA+AGG - chr2.1:18578603-18578622 None:intergenic 35.0%
TGATCTTTGCGTATAATGGT+TGG - chr2.1:18577479-18577498 None:intergenic 35.0%
TGTGGTAACTTCGTTATCAT+CGG - chr2.1:18577623-18577642 None:intergenic 35.0%
TTCAAGTGATGAGTTGTAAG+AGG - chr2.1:18577455-18577474 None:intergenic 35.0%
TTCTAGCCGTTTCTATCAAT+CGG - chr2.1:18577918-18577937 None:intergenic 35.0%
TTGCTCCATGATCTATATCT+CGG - chr2.1:18577691-18577710 None:intergenic 35.0%
! GTGTTGATGTGAATAATCCA+AGG + chr2.1:18578496-18578515 MS.gene031301:CDS 35.0%
! TTTCATGGGATTCCTGTTTT+CGG + chr2.1:18578381-18578400 MS.gene031301:CDS 35.0%
AAGCAGTGCATGTAGAGAAA+CGG + chr2.1:18577782-18577801 MS.gene031301:CDS 40.0%
AAGGATCCAAGCAAACCATT+TGG - chr2.1:18577307-18577326 None:intergenic 40.0%
AATCTCAAAAGTCCATCCCT+TGG - chr2.1:18578516-18578535 None:intergenic 40.0%
AATTCAGGAGAAATCCTCTC+TGG + chr2.1:18577421-18577440 MS.gene031301:CDS 40.0%
ACACAACACCTTTCTTCAAC+AGG + chr2.1:18578592-18578611 MS.gene031301:CDS 40.0%
AGATTGCTCAAGGAACTCAA+TGG + chr2.1:18578196-18578215 MS.gene031301:CDS 40.0%
AGCATTAAGGAAACAGCATC+CGG + chr2.1:18577664-18577683 MS.gene031301:CDS 40.0%
ATCTCTACAAGGGTTTCCAT+CGG + chr2.1:18577825-18577844 MS.gene031301:CDS 40.0%
ATGAGAGGAAAACGGTGAAT+CGG - chr2.1:18577353-18577372 None:intergenic 40.0%
CAAGTGATGAGTTGTAAGAG+GGG - chr2.1:18577453-18577472 None:intergenic 40.0%
CTAGGTGCTTATATGGTAGA+AGG + chr2.1:18577973-18577992 MS.gene031301:CDS 40.0%
GAAGAGGATTCATCTCTACA+AGG + chr2.1:18577814-18577833 MS.gene031301:CDS 40.0%
GACATGCTTGATATCAGACA+CGG + chr2.1:18578420-18578439 MS.gene031301:CDS 40.0%
GATGTGAATAATCCAAGGGA+TGG + chr2.1:18578501-18578520 MS.gene031301:CDS 40.0%
GCCGAATTTCTTAGACATCA+AGG - chr2.1:18578349-18578368 None:intergenic 40.0%
GTGGTAACTTCGTTATCATC+GGG - chr2.1:18577622-18577641 None:intergenic 40.0%
GTTTCCAAAGCCAACTTTCA+AGG - chr2.1:18577326-18577345 None:intergenic 40.0%
GTTTGATTCTTGCTCCACAA+GGG - chr2.1:18578568-18578587 None:intergenic 40.0%
TAATGTGGAGCAGCATTGTT+TGG + chr2.1:18578695-18578714 MS.gene031301:CDS 40.0%
TACTTGGTAAGTGGAAGTCT+AGG + chr2.1:18578778-18578797 MS.gene031301:CDS 40.0%
TAGCAAGGAGAGGATTAATG+TGG + chr2.1:18578680-18578699 MS.gene031301:CDS 40.0%
TCTCAAGAAATAGCAAGGAG+AGG + chr2.1:18578670-18578689 MS.gene031301:CDS 40.0%
TGCTCCATGATCTATATCTC+GGG - chr2.1:18577690-18577709 None:intergenic 40.0%
TGGTAACTTCGTTATCATCG+GGG - chr2.1:18577621-18577640 None:intergenic 40.0%
TGTTTGATTCTTGCTCCACA+AGG - chr2.1:18578569-18578588 None:intergenic 40.0%
TTGCTTGGATCCTTGAAAGT+TGG + chr2.1:18577313-18577332 MS.gene031301:CDS 40.0%
TTTGAACATATTGCGACCCT+TGG - chr2.1:18577720-18577739 None:intergenic 40.0%
! TCTTTTCCATCACTGCCAAA+TGG + chr2.1:18577289-18577308 MS.gene031301:CDS 40.0%
!! AGCAGTTTCTAGTTCCAACA+AGG - chr2.1:18577590-18577609 None:intergenic 40.0%
!! CTCCTTGCTATTTCTTGAGA+GGG - chr2.1:18578670-18578689 None:intergenic 40.0%
!! TCTCCTTGCTATTTCTTGAG+AGG - chr2.1:18578671-18578690 None:intergenic 40.0%
!! TTCATTGGGTGAGAGCATTA+AGG + chr2.1:18577651-18577670 MS.gene031301:CDS 40.0%
!!! AAGGGATGGACTTTTGAGAT+TGG + chr2.1:18578515-18578534 MS.gene031301:CDS 40.0%
!!! TGTCGGATTTTCTGGTTACT+TGG - chr2.1:18577565-18577584 None:intergenic 40.0%
!!! TTCTGTGTTGTTTTCAGCCA+TGG - chr2.1:18577887-18577906 None:intergenic 40.0%
AAAGATGGGGCAATGCTCTT+AGG + chr2.1:18578903-18578922 MS.gene031301:CDS 45.0%
AAGATGGGGCAATGCTCTTA+GGG + chr2.1:18578904-18578923 MS.gene031301:CDS 45.0%
AATTGCTTCAAATCGCCCGA+TGG - chr2.1:18577844-18577863 None:intergenic 45.0%
AGCAAACCATTTGGCAGTGA+TGG - chr2.1:18577298-18577317 None:intergenic 45.0%
AGCATGTCTCTTGTCACATC+TGG - chr2.1:18578408-18578427 None:intergenic 45.0%
ATTCCTGCAAGCTTCAGCAA+TGG - chr2.1:18578148-18578167 None:intergenic 45.0%
ATTTGATTGCCTGCTACTGC+TGG - chr2.1:18577761-18577780 None:intergenic 45.0%
CACCCTCTCAAGAAATAGCA+AGG + chr2.1:18578665-18578684 MS.gene031301:CDS 45.0%
CTACTGCTGGTAACATGTGA+CGG - chr2.1:18577748-18577767 None:intergenic 45.0%
GAAGTCTCTCTCTCCTAAAG+TGG + chr2.1:18578539-18578558 MS.gene031301:CDS 45.0%
GAATCCCATGAAACTCAACC+GGG - chr2.1:18578374-18578393 None:intergenic 45.0%
GACACGAACTACTTGGTAAG+TGG + chr2.1:18578769-18578788 MS.gene031301:CDS 45.0%
GATCACAGGAATCGATGATC+CGG + chr2.1:18578272-18578291 MS.gene031301:CDS 45.0%
GGAACTAGAAACTGCTCTGA+TGG + chr2.1:18577594-18577613 MS.gene031301:CDS 45.0%
GGAATCCCATGAAACTCAAC+CGG - chr2.1:18578375-18578394 None:intergenic 45.0%
GGTAACTTCGTTATCATCGG+GGG - chr2.1:18577620-18577639 None:intergenic 45.0%
GTCAATGTCATTGCATGCGA+AGG + chr2.1:18578729-18578748 MS.gene031301:CDS 45.0%
GTCTAAGAAATTCGGCATCC+CGG + chr2.1:18578353-18578372 MS.gene031301:CDS 45.0%
GTGAGTAATTCTTCGCCTTC+AGG - chr2.1:18578060-18578079 None:intergenic 45.0%
TATCAGACACGGAGAAGCTT+TGG + chr2.1:18578431-18578450 MS.gene031301:CDS 45.0%
TCTAAATATGCCCGTGGCAA+GGG + chr2.1:18578297-18578316 MS.gene031301:CDS 45.0%
TCTCTACAAGGGTTTCCATC+GGG + chr2.1:18577826-18577845 MS.gene031301:CDS 45.0%
TGAGAGGAAAACGGTGAATC+GGG - chr2.1:18577352-18577371 None:intergenic 45.0%
TGCATGCGAAGGTAAGGAAA+GGG + chr2.1:18578740-18578759 MS.gene031301:CDS 45.0%
TGTCATTGCATGCGAAGGTA+AGG + chr2.1:18578734-18578753 MS.gene031301:CDS 45.0%
TTCAGGCTCTCTGCACTTAA+GGG - chr2.1:18578043-18578062 None:intergenic 45.0%
TTGCATGCGAAGGTAAGGAA+AGG + chr2.1:18578739-18578758 MS.gene031301:CDS 45.0%
TTTGGTTACATGGCTGCCAA+TGG + chr2.1:18578120-18578139 MS.gene031301:CDS 45.0%
! AAGTCTAGGTTGACAATGGC+TGG + chr2.1:18578792-18578811 MS.gene031301:CDS 45.0%
! GCTCTGAATAGCATCTCAGA+AGG - chr2.1:18578866-18578885 None:intergenic 45.0%
! GGATCCTTGAAAGTTGGCTT+TGG + chr2.1:18577319-18577338 MS.gene031301:CDS 45.0%
! GTGGAAGTCTAGGTTGACAA+TGG + chr2.1:18578788-18578807 MS.gene031301:CDS 45.0%
!! AGAAGGTCTTGTTGCAAGGA+CGG + chr2.1:18577990-18578009 MS.gene031301:CDS 45.0%
!! GTTGTTTTCAGCCATGGCTT+TGG - chr2.1:18577881-18577900 None:intergenic 45.0%
!! TCACTGCCAAATGGTTTGCT+TGG + chr2.1:18577298-18577317 MS.gene031301:CDS 45.0%
!! TGGTAGAAGGTCTTGTTGCA+AGG + chr2.1:18577986-18578005 MS.gene031301:CDS 45.0%
AAGAGGGGCTTATACCAGAG+AGG - chr2.1:18577438-18577457 None:intergenic 50.0%
AGCCACGAGCAACACAATTC+AGG + chr2.1:18577406-18577425 MS.gene031301:CDS 50.0%
AGCTTCAGCAATGGCTCCAT+TGG - chr2.1:18578139-18578158 None:intergenic 50.0%
CACATGTTACCAGCAGTAGC+AGG + chr2.1:18577749-18577768 MS.gene031301:CDS 50.0%
CACTCTCATCAGCTGTGTGA+TGG - chr2.1:18578479-18578498 None:intergenic 50.0%
CAGTGCATGTAGAGAAACGG+CGG + chr2.1:18577785-18577804 MS.gene031301:CDS 50.0%
CATGGAGCAATGAGCACCAA+GGG + chr2.1:18577701-18577720 MS.gene031301:CDS 50.0%
CCACGGGCATATTTAGAGAC+CGG - chr2.1:18578294-18578313 None:intergenic 50.0%
CCAGAGGCTAGGTGCTTATA+TGG + chr2.1:18577966-18577985 MS.gene031301:CDS 50.0%
CCATATAAGCACCTAGCCTC+TGG - chr2.1:18577969-18577988 None:intergenic 50.0%
CCATGATCTATATCTCGGGC+CGG - chr2.1:18577686-18577705 None:intergenic 50.0%
CTACTTCTAGTCCCTTGCCA+CGG - chr2.1:18578311-18578330 None:intergenic 50.0%
CTAGTTCCAACAAGGCGTGT+CGG - chr2.1:18577582-18577601 None:intergenic 50.0%
CTCTAAATATGCCCGTGGCA+AGG + chr2.1:18578296-18578315 MS.gene031301:CDS 50.0%
CTTAAGTGCAGAGAGCCTGA+AGG + chr2.1:18578042-18578061 MS.gene031301:CDS 50.0%
CTTCAGGCTCTCTGCACTTA+AGG - chr2.1:18578044-18578063 None:intergenic 50.0%
GAAAATCCGACACGCCTTGT+TGG + chr2.1:18577573-18577592 MS.gene031301:CDS 50.0%
GATTGCATGTGCCAAAGCCA+TGG + chr2.1:18577867-18577886 MS.gene031301:CDS 50.0%
GCATCCCGGTTGAGTTTCAT+GGG + chr2.1:18578367-18578386 MS.gene031301:CDS 50.0%
GGCAAGGGACTAGAAGTAGT+TGG + chr2.1:18578312-18578331 MS.gene031301:CDS 50.0%
GTCACATCTGGACCGAAAAC+AGG - chr2.1:18578396-18578415 None:intergenic 50.0%
GTTGAGCGACACGAACTACT+TGG + chr2.1:18578762-18578781 MS.gene031301:CDS 50.0%
TAAGCACCTAGCCTCTGGAT+TGG - chr2.1:18577964-18577983 None:intergenic 50.0%
TACTTCTAGTCCCTTGCCAC+GGG - chr2.1:18578310-18578329 None:intergenic 50.0%
TCAATGGGGAACCAATCCAG+AGG + chr2.1:18577950-18577969 MS.gene031301:CDS 50.0%
TCATGGAGCAATGAGCACCA+AGG + chr2.1:18577700-18577719 MS.gene031301:CDS 50.0%
TCCACAAGGGTGACCACTTT+AGG - chr2.1:18578555-18578574 None:intergenic 50.0%
TCCTAAAGTGGTCACCCTTG+TGG + chr2.1:18578551-18578570 MS.gene031301:CDS 50.0%
TCTTGAGAGGGTGACATCGA+TGG - chr2.1:18578658-18578677 None:intergenic 50.0%
TGCTGAAGCTTGCAGGAATG+AGG + chr2.1:18578149-18578168 MS.gene031301:CDS 50.0%
TGGAGCAGCATTGTTTGGCT+CGG + chr2.1:18578700-18578719 MS.gene031301:CDS 50.0%
TGTAGAGAAACGGCGGAAGT+TGG + chr2.1:18577792-18577811 MS.gene031301:CDS 50.0%
! ACAAGGCGTGTCGGATTTTC+TGG - chr2.1:18577573-18577592 None:intergenic 50.0%
! CTCCTGAATTGTGTTGCTCG+TGG - chr2.1:18577411-18577430 None:intergenic 50.0%
!! TTGCAAGGACGGAAGCATCA+GGG + chr2.1:18578001-18578020 MS.gene031301:CDS 50.0%
ATGGGGCAATGCTCTTAGGG+TGG + chr2.1:18578907-18578926 MS.gene031301:CDS 55.0%
ATTCCTGTGATCCGCACGTG+GGG - chr2.1:18578264-18578283 None:intergenic 55.0%
CCGGCCCGAGATATAGATCA+TGG + chr2.1:18577683-18577702 MS.gene031301:CDS 55.0%
CCGGTCTCTAAATATGCCCG+TGG + chr2.1:18578291-18578310 MS.gene031301:CDS 55.0%
GAAACGGCGGAAGTTGGAAG+AGG + chr2.1:18577798-18577817 MS.gene031301:CDS 55.0%
GAGCCATTGCTGAAGCTTGC+AGG + chr2.1:18578142-18578161 MS.gene031301:CDS 55.0%
GATTCCTGTGATCCGCACGT+GGG - chr2.1:18578265-18578284 None:intergenic 55.0%
GGCATCCCGGTTGAGTTTCA+TGG + chr2.1:18578366-18578385 MS.gene031301:CDS 55.0%
! CAGACACGGAGAAGCTTTGG+CGG + chr2.1:18578434-18578453 MS.gene031301:CDS 55.0%
! GCTGTGTGATGGAGTTGCAG+TGG - chr2.1:18578468-18578487 None:intergenic 55.0%
! GGAGCAGCATTGTTTGGCTC+GGG + chr2.1:18578701-18578720 MS.gene031301:CDS 55.0%
!! GTTGCAAGGACGGAAGCATC+AGG + chr2.1:18578000-18578019 MS.gene031301:CDS 55.0%
CAAGCTCTTGCCGCAAGACC+TGG + chr2.1:18578231-18578250 MS.gene031301:CDS 60.0%
CGATTCCTGTGATCCGCACG+TGG - chr2.1:18578266-18578285 None:intergenic 60.0%
GGGGAACCAATCCAGAGGCT+AGG + chr2.1:18577955-18577974 MS.gene031301:CDS 60.0%
CTCTTGCCGCAAGACCTGGC+GGG + chr2.1:18578235-18578254 MS.gene031301:CDS 65.0%
GCTCTTGCCGCAAGACCTGG+CGG + chr2.1:18578234-18578253 MS.gene031301:CDS 65.0%
TCTTGCCGCAAGACCTGGCG+GGG + chr2.1:18578236-18578255 MS.gene031301:CDS 65.0%
GCACCCCACGTGCGGATCAC+AGG + chr2.1:18578258-18578277 MS.gene031301:CDS 70.0%
GGGTGCCCCGCCAGGTCTTG+CGG - chr2.1:18578244-18578263 None:intergenic 75.0%
!! CTGGCGGGGCACCCCACGTG+CGG + chr2.1:18578250-18578269 MS.gene031301:CDS 80.0%
!! CGCACGTGGGGTGCCCCGCC+AGG - chr2.1:18578252-18578271 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 18577229 18578968 18577229 ID=MS.gene031301
chr2.1 mRNA 18577229 18578968 18577229 ID=MS.gene031301.t1;Parent=MS.gene031301
chr2.1 exon 18577229 18578968 18577229 ID=MS.gene031301.t1.exon1;Parent=MS.gene031301.t1
chr2.1 CDS 18577229 18578968 18577229 ID=cds.MS.gene031301.t1;Parent=MS.gene031301.t1
Gene Sequence

>MS.gene031301

ATGGACTCGCAGCAACTTTATAGTTACAGTATGAGTAATGCAGGATTACCTTACATGTCATCTTTTCCATCACTGCCAAATGGTTTGCTTGGATCCTTGAAAGTTGGCTTTGGAAACTCACCCGATTCACCGTTTTCCTCTCATTTTGATTCTGATACTCTTTCTGCATTTAGTGACAGCCACGAGCAACACAATTCAGGAGAAATCCTCTCTGGTATAAGCCCCTCTTACAACTCATCACTTGAAACCAACCATTATACGCAAAGATCATTCTCTTCGATCAACAGTCTCAAAGACAGTTTGCAACTATATTCTGCTAGAAATTCTTTTCTACCAAGTAACCAGAAAATCCGACACGCCTTGTTGGAACTAGAAACTGCTCTGATGGCCCCCGATGATAACGAAGTTACCACATCTAATTCTTCATTGGGTGAGAGCATTAAGGAAACAGCATCCGGCCCGAGATATAGATCATGGAGCAATGAGCACCAAGGGTCGCAATATGTTCAAAGTCAACCGTCACATGTTACCAGCAGTAGCAGGCAATCAAATGAAGCAGTGCATGTAGAGAAACGGCGGAAGTTGGAAGAGGATTCATCTCTACAAGGGTTTCCATCGGGCGATTTGAAGCAATTACTGATTGCATGTGCCAAAGCCATGGCTGAAAACAACACAGAAGATTTTGACCGATTGATAGAAACGGCTAGAAATGCTGTGTCTATCAATGGGGAACCAATCCAGAGGCTAGGTGCTTATATGGTAGAAGGTCTTGTTGCAAGGACGGAAGCATCAGGGAATAGTATCTATCATGCCCTTAAGTGCAGAGAGCCTGAAGGCGAAGAATTACTCACTTACATGCAACTTCTTTTCGAAATCTGTCCCTACTTAAAATTTGGTTACATGGCTGCCAATGGAGCCATTGCTGAAGCTTGCAGGAATGAGGATCACATACACATCATAGACTTTCAGATTGCTCAAGGAACTCAATGGATGACACTTCTTCAAGCTCTTGCCGCAAGACCTGGCGGGGCACCCCACGTGCGGATCACAGGAATCGATGATCCGGTCTCTAAATATGCCCGTGGCAAGGGACTAGAAGTAGTTGGAGAGAGATTGTCCTTGATGTCTAAGAAATTCGGCATCCCGGTTGAGTTTCATGGGATTCCTGTTTTCGGTCCAGATGTGACAAGAGACATGCTTGATATCAGACACGGAGAAGCTTTGGCGGTGAATTTTCCACTGCAACTCCATCACACAGCTGATGAGAGTGTTGATGTGAATAATCCAAGGGATGGACTTTTGAGATTGGTGAAGTCTCTCTCTCCTAAAGTGGTCACCCTTGTGGAGCAAGAATCAAACACAAACACAACACCTTTCTTCAACAGGTTCATAGAAACTTTAGATTACTACTTGGCAATCTTCGAGTCCATCGATGTCACCCTCTCAAGAAATAGCAAGGAGAGGATTAATGTGGAGCAGCATTGTTTGGCTCGGGATATCGTCAATGTCATTGCATGCGAAGGTAAGGAAAGGGTTGAGCGACACGAACTACTTGGTAAGTGGAAGTCTAGGTTGACAATGGCTGGATTTCGTCAATGTCCTTTGAGTTCTTATGTGAATTCTGTTATAAGAAGCCTTCTGAGATGCTATTCAGAGCACTATACACTAGTTGAGAAAGATGGGGCAATGCTCTTAGGGTGGAAGAACAGAAATTTGATATCTGCTTCAGCTTGGCATTGA
Protein sequence

>MS.gene031301.t1

MDSQQLYSYSMSNAGLPYMSSFPSLPNGLLGSLKVGFGNSPDSPFSSHFDSDTLSAFSDSHEQHNSGEILSGISPSYNSSLETNHYTQRSFSSINSLKDSLQLYSARNSFLPSNQKIRHALLELETALMAPDDNEVTTSNSSLGESIKETASGPRYRSWSNEHQGSQYVQSQPSHVTSSSRQSNEAVHVEKRRKLEEDSSLQGFPSGDLKQLLIACAKAMAENNTEDFDRLIETARNAVSINGEPIQRLGAYMVEGLVARTEASGNSIYHALKCREPEGEELLTYMQLLFEICPYLKFGYMAANGAIAEACRNEDHIHIIDFQIAQGTQWMTLLQALAARPGGAPHVRITGIDDPVSKYARGKGLEVVGERLSLMSKKFGIPVEFHGIPVFGPDVTRDMLDIRHGEALAVNFPLQLHHTADESVDVNNPRDGLLRLVKSLSPKVVTLVEQESNTNTTPFFNRFIETLDYYLAIFESIDVTLSRNSKERINVEQHCLARDIVNVIACEGKERVERHELLGKWKSRLTMAGFRQCPLSSYVNSVIRSLLRCYSEHYTLVEKDGAMLLGWKNRNLISASAWH