AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031499

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031499.t1 MTR_4g081710 97.647 340 7 1 1 340 1 339 0.0 681
MS.gene031499.t1 MTR_5g041350 61.932 352 116 6 4 340 2 350 7.14e-129 372
MS.gene031499.t1 MTR_5g041350 54.406 261 102 5 94 340 4 261 4.30e-69 217
MS.gene031499.t1 MTR_2g086450 49.791 239 99 5 10 238 38 265 4.85e-67 216
MS.gene031499.t1 MTR_6g032990 40.108 369 172 10 10 338 35 394 1.79e-62 204
MS.gene031499.t1 MTR_5g041350 53.719 242 95 5 113 340 1 239 3.19e-60 193
MS.gene031499.t1 MTR_6g444980 51.381 181 73 5 10 184 28 199 8.22e-52 176
MS.gene031499.t1 MTR_5g027440 46.809 188 88 4 7 190 15 194 2.71e-48 164
MS.gene031499.t1 MTR_6g444980 55.245 143 54 3 10 151 28 161 1.03e-45 159
MS.gene031499.t1 MTR_7g088070 50.920 163 70 3 16 178 49 201 5.01e-45 155
MS.gene031499.t1 MTR_7g088070 50.307 163 67 4 16 178 49 197 1.31e-43 151
MS.gene031499.t1 MTR_2g027860 48.148 162 60 4 17 171 49 193 1.55e-42 150
MS.gene031499.t1 MTR_2g027800 40.417 240 115 8 10 233 32 259 4.37e-41 146
MS.gene031499.t1 MTR_2g027800 39.662 237 107 7 10 233 32 245 1.11e-39 142
MS.gene031499.t1 MTR_1g090670 40.870 230 105 8 10 232 37 242 1.43e-39 141
MS.gene031499.t1 MTR_7g098250 42.424 165 78 4 13 175 258 407 2.59e-36 137
MS.gene031499.t1 MTR_1g080330 43.210 162 72 4 13 168 171 318 8.60e-35 131
MS.gene031499.t1 MTR_7g115530 37.696 191 91 4 17 204 218 383 9.03e-35 132
MS.gene031499.t1 MTR_1g053835 42.282 149 71 3 13 160 254 388 3.80e-33 127
MS.gene031499.t1 MTR_7g093030 38.095 147 75 3 16 160 211 343 5.19e-29 115
MS.gene031499.t1 MTR_7g115530 45.528 123 50 3 17 136 218 326 7.58e-27 108
MS.gene031499.t1 MTR_1g080330 43.651 126 54 3 13 135 171 282 2.50e-26 106
MS.gene031499.t1 MTR_7g068600 39.041 146 71 4 13 153 9 141 2.43e-25 102
MS.gene031499.t1 MTR_1g053800 37.762 143 77 3 12 154 172 302 1.07e-21 94.4
MS.gene031499.t1 MTR_7g115530 53.333 75 33 1 17 89 218 292 6.87e-21 91.7
MS.gene031499.t1 MTR_7g115530 47.436 78 36 1 17 89 218 295 2.91e-20 90.5
MS.gene031499.t1 MTR_7g093030 55.172 58 26 0 16 73 211 268 1.01e-18 85.5
MS.gene031499.t1 MTR_7g115530 61.538 52 20 0 17 68 218 269 3.49e-18 83.6
MS.gene031499.t1 MTR_7g115530 58.491 53 22 0 17 69 218 270 1.99e-17 81.6
MS.gene031499.t1 MTR_1g090670 36.649 191 90 9 49 232 1 167 3.14e-17 79.7
MS.gene031499.t1 MTR_1g053830 47.541 61 32 0 13 73 14 74 4.35e-17 76.3
MS.gene031499.t1 MTR_6g071625 40.860 93 49 2 18 104 203 295 1.15e-15 77.4
MS.gene031499.t1 MTR_8g066310 37.838 111 67 2 18 128 174 282 1.27e-15 77.4
MS.gene031499.t1 MTR_7g069660 56.604 53 23 0 18 70 72 124 2.90e-15 76.3
MS.gene031499.t1 MTR_3g450310 49.180 61 31 0 17 77 19 79 9.14e-15 72.8
MS.gene031499.t1 MTR_0223s0040 45.679 81 41 1 18 95 19 99 9.43e-15 73.9
MS.gene031499.t1 MTR_1g112370 60.377 53 21 0 18 70 23 75 9.69e-15 74.3
MS.gene031499.t1 MTR_2g023580 50.000 60 30 0 18 77 56 115 9.87e-15 73.2
MS.gene031499.t1 MTR_4g121020 55.932 59 25 1 13 71 211 268 2.38e-14 74.3
MS.gene031499.t1 MTR_4g121020 55.932 59 25 1 13 71 211 268 2.44e-14 74.3
MS.gene031499.t1 MTR_4g121020 55.932 59 25 1 13 71 107 164 2.53e-14 74.3
MS.gene031499.t1 MTR_2g034960 52.857 70 26 2 13 76 207 275 3.46e-14 73.9
MS.gene031499.t1 MTR_5g029470 44.048 84 46 1 18 101 220 302 3.61e-14 73.2
MS.gene031499.t1 MTR_4g021790 54.545 55 24 1 17 71 168 221 5.15e-14 71.6
MS.gene031499.t1 MTR_3g064840 47.826 69 36 0 18 86 275 343 5.42e-14 73.2
MS.gene031499.t1 MTR_1g021520 52.632 57 27 0 18 74 127 183 9.13e-14 71.2
MS.gene031499.t1 MTR_8g086410 51.786 56 27 0 18 73 110 165 1.11e-13 72.0
MS.gene031499.t1 MTR_8g086410 51.786 56 27 0 18 73 112 167 1.11e-13 72.0
MS.gene031499.t1 MTR_5g017980 42.697 89 50 1 17 105 193 280 1.30e-13 71.2
MS.gene031499.t1 MTR_1g032570 52.542 59 27 1 13 71 127 184 1.58e-13 71.6
MS.gene031499.t1 MTR_1g032570 52.542 59 27 1 13 71 196 253 1.68e-13 71.6
MS.gene031499.t1 MTR_2g016220 54.717 53 24 0 18 70 90 142 1.73e-13 71.2
MS.gene031499.t1 MTR_2g084230 50.794 63 28 1 18 77 98 160 1.76e-13 71.2
MS.gene031499.t1 MTR_2g084230 50.794 63 28 1 18 77 98 160 1.81e-13 70.5
MS.gene031499.t1 MTR_2g016220 54.717 53 24 0 18 70 90 142 2.02e-13 70.9
MS.gene031499.t1 MTR_1g093080 58.182 55 23 0 17 71 211 265 9.20e-13 68.9
MS.gene031499.t1 MTR_4g131600 49.153 59 29 1 13 71 197 254 1.13e-12 69.3
MS.gene031499.t1 MTR_7g093010 32.593 135 81 2 17 151 213 337 1.20e-12 68.6
MS.gene031499.t1 MTR_8g086410 43.077 65 33 1 18 78 112 176 1.73e-12 68.2
MS.gene031499.t1 MTR_4g021855 52.727 55 25 1 17 71 168 221 2.34e-12 67.0
MS.gene031499.t1 MTR_2g016220 45.161 62 33 1 18 78 90 151 4.22e-12 67.0
MS.gene031499.t1 MTR_2g450070 49.153 59 29 1 13 71 209 266 5.43e-12 67.4
MS.gene031499.t1 MTR_2g450070 49.153 59 29 1 13 71 209 266 5.87e-12 67.0
MS.gene031499.t1 MTR_4g021760 51.852 54 25 1 17 70 169 221 7.37e-12 65.9
MS.gene031499.t1 MTR_7g089010 52.727 55 25 1 17 71 91 144 1.13e-11 65.1
MS.gene031499.t1 MTR_4g086835 44.000 75 41 1 14 88 271 344 1.27e-11 65.5
MS.gene031499.t1 MTR_4g064730 43.421 76 36 2 14 83 128 202 1.30e-11 65.1
MS.gene031499.t1 MTR_1g013170 43.056 72 38 2 17 88 191 259 2.14e-11 65.1
MS.gene031499.t1 MTR_4g113140 50.000 58 29 0 14 71 232 289 8.93e-11 63.2
MS.gene031499.t1 MTR_5g054300 52.727 55 26 0 17 71 198 252 9.34e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031499.t1 AT1G69580 52.751 309 108 9 1 297 18 300 5.53e-82 253
MS.gene031499.t1 AT1G69580 51.935 310 110 9 1 297 18 301 2.46e-80 248
MS.gene031499.t1 AT3G04030 50.855 234 98 5 10 235 38 262 3.12e-64 209
MS.gene031499.t1 AT5G18240 49.138 232 105 5 10 235 38 262 3.27e-62 204
MS.gene031499.t1 AT5G18240 49.138 232 105 5 10 235 38 262 3.27e-62 204
MS.gene031499.t1 AT3G04030 50.000 234 99 6 10 235 38 261 4.60e-61 200
MS.gene031499.t1 AT5G18240 48.276 232 105 6 10 235 38 260 7.81e-59 195
MS.gene031499.t1 AT3G24120 45.852 229 111 5 10 229 34 258 3.02e-58 190
MS.gene031499.t1 AT4G13640 48.276 232 99 8 10 230 30 251 6.14e-58 189
MS.gene031499.t1 AT3G04030 48.718 234 97 6 10 235 38 256 1.46e-57 191
MS.gene031499.t1 AT3G24120 45.259 232 111 6 10 229 34 261 4.92e-56 184
MS.gene031499.t1 AT4G13640 47.660 235 99 9 10 230 30 254 9.28e-56 184
MS.gene031499.t1 AT5G18240 46.983 232 104 6 10 235 38 256 1.45e-55 186
MS.gene031499.t1 AT5G18240 46.983 232 104 6 10 235 38 256 1.45e-55 186
MS.gene031499.t1 AT1G79430 53.247 154 64 1 10 163 27 172 1.17e-49 170
MS.gene031499.t1 AT5G45580 48.428 159 75 3 10 161 16 174 2.54e-44 154
MS.gene031499.t1 AT5G45580 48.734 158 74 3 11 161 1 158 2.60e-44 153
MS.gene031499.t1 AT2G01060 46.584 161 70 2 16 173 14 161 2.37e-43 151
MS.gene031499.t1 AT3G12730 50.000 152 71 3 10 160 16 163 1.24e-42 148
MS.gene031499.t1 AT3G04030 46.154 195 88 5 49 235 1 186 8.13e-39 140
MS.gene031499.t1 AT3G04450 40.571 175 94 3 13 186 194 359 9.38e-35 131
MS.gene031499.t1 AT3G04450 40.571 175 94 3 13 186 194 359 9.38e-35 131
MS.gene031499.t1 AT3G04450 40.571 175 94 3 13 186 234 399 2.01e-34 131
MS.gene031499.t1 AT5G29000 43.210 162 72 3 16 171 187 334 2.60e-34 129
MS.gene031499.t1 AT5G29000 43.210 162 72 3 16 171 187 334 2.60e-34 129
MS.gene031499.t1 AT4G28610 42.949 156 75 3 16 169 224 367 3.62e-34 130
MS.gene031499.t1 AT5G29000 43.210 162 72 3 16 171 230 377 6.42e-34 129
MS.gene031499.t1 AT3G04030 43.590 195 87 6 49 235 1 180 3.51e-32 122
MS.gene031499.t1 AT5G06800 42.466 146 71 3 17 159 192 327 7.20e-32 123
MS.gene031499.t1 AT3G13040 37.037 189 105 3 13 197 237 415 7.31e-31 121
MS.gene031499.t1 AT3G13040 37.037 189 105 3 13 197 237 415 7.31e-31 121
MS.gene031499.t1 AT3G13040 37.037 189 105 3 13 197 237 415 7.31e-31 121
MS.gene031499.t1 AT5G18240 41.969 193 93 6 49 235 1 180 7.85e-31 119
MS.gene031499.t1 AT5G06800 41.259 143 72 3 17 159 192 322 8.92e-31 120
MS.gene031499.t1 AT2G20400 40.541 148 72 2 19 163 233 367 8.12e-30 118
MS.gene031499.t1 AT2G20400 41.216 148 70 3 19 163 233 366 3.22e-29 116
MS.gene031499.t1 AT1G79430 47.826 115 52 1 49 163 1 107 1.85e-26 107
MS.gene031499.t1 AT2G01060 41.600 125 57 2 52 173 1 112 3.58e-22 94.0
MS.gene031499.t1 AT3G04450 57.377 61 26 0 13 73 234 294 6.88e-20 89.0
MS.gene031499.t1 AT5G29000 56.897 58 25 0 16 73 230 287 2.76e-19 87.4
MS.gene031499.t1 AT3G12730 43.363 113 59 3 49 160 1 109 6.76e-19 83.6
MS.gene031499.t1 AT5G06800 54.386 57 26 0 17 73 192 248 9.35e-18 82.4
MS.gene031499.t1 AT2G06020 50.000 64 32 0 10 73 79 142 5.70e-17 80.5
MS.gene031499.t1 AT4G17695 49.275 69 35 0 18 86 165 233 3.60e-15 75.9
MS.gene031499.t1 AT1G32240 37.864 103 64 0 18 120 214 316 4.29e-15 75.9
MS.gene031499.t1 AT1G32240 37.864 103 64 0 18 120 214 316 4.54e-15 75.9
MS.gene031499.t1 AT1G14600 56.604 53 23 0 18 70 24 76 6.64e-15 73.9
MS.gene031499.t1 AT4G04605 52.830 53 25 0 18 70 15 67 1.55e-14 72.4
MS.gene031499.t1 AT2G38300 56.604 53 23 0 18 70 55 107 3.53e-14 72.8
MS.gene031499.t1 AT5G42630 49.231 65 27 1 18 82 106 164 3.70e-14 71.2
MS.gene031499.t1 AT5G16560 54.386 57 26 0 18 74 220 276 4.08e-14 73.2
MS.gene031499.t1 AT2G02060 49.180 61 31 0 18 78 30 90 7.35e-14 70.9
MS.gene031499.t1 AT2G02060 49.180 61 31 0 18 78 30 90 7.35e-14 70.9
MS.gene031499.t1 AT3G16857 56.364 55 23 1 17 71 167 220 7.81e-14 72.8
MS.gene031499.t1 AT5G42630 49.231 65 27 1 18 82 106 164 7.86e-14 71.2
MS.gene031499.t1 AT3G16857 56.364 55 23 1 17 71 237 290 8.79e-14 72.8
MS.gene031499.t1 AT3G16857 56.364 55 23 1 17 71 237 290 9.40e-14 72.8
MS.gene031499.t1 AT4G04580 52.830 53 25 0 18 70 15 67 1.04e-13 68.6
MS.gene031499.t1 AT2G42660 50.943 53 26 0 18 70 51 103 2.36e-13 69.3
MS.gene031499.t1 AT2G40260 49.153 59 30 0 18 76 83 141 3.84e-13 70.5
MS.gene031499.t1 AT5G45580 39.423 104 56 3 65 161 16 119 1.49e-12 66.6
MS.gene031499.t1 AT3G25790 47.619 63 33 0 22 84 201 263 1.85e-12 67.8
MS.gene031499.t1 AT5G59570 50.000 58 28 1 14 71 138 194 1.89e-12 67.4
MS.gene031499.t1 AT5G59570 50.000 58 28 1 14 71 138 194 1.89e-12 67.4
MS.gene031499.t1 AT5G05090 52.727 55 25 1 17 71 81 134 3.18e-12 66.2
MS.gene031499.t1 AT3G10760 52.727 55 25 1 17 71 105 158 4.06e-12 66.6
MS.gene031499.t1 AT2G40970 51.724 58 27 1 14 71 102 158 6.33e-12 65.1
MS.gene031499.t1 AT5G58080 53.448 58 26 1 14 71 191 247 6.59e-12 67.0
MS.gene031499.t1 AT3G46640 50.909 55 26 1 17 71 144 197 1.07e-11 65.5
MS.gene031499.t1 AT3G46640 50.909 55 26 1 17 71 144 197 1.08e-11 65.5
MS.gene031499.t1 AT3G46640 50.909 55 26 1 17 71 144 197 1.08e-11 65.5
MS.gene031499.t1 AT4G37180 50.943 53 26 0 19 71 212 264 1.14e-11 65.5
MS.gene031499.t1 AT4G37180 50.943 53 26 0 19 71 219 271 1.19e-11 65.5
MS.gene031499.t1 AT2G25180 38.298 94 51 2 17 104 195 287 1.29e-11 65.9
MS.gene031499.t1 AT2G01760 49.091 55 27 1 17 71 135 188 1.63e-11 64.7
MS.gene031499.t1 AT2G01760 49.091 55 27 1 17 71 200 253 2.26e-11 64.7
MS.gene031499.t1 AT1G67710 49.057 53 27 0 19 71 121 173 2.68e-11 64.7
MS.gene031499.t1 AT2G01760 49.091 55 27 1 17 71 228 281 3.29e-11 64.3
MS.gene031499.t1 AT1G67710 49.057 53 27 0 19 71 130 182 3.74e-11 64.3
MS.gene031499.t1 AT1G67710 49.057 53 27 0 19 71 195 247 3.92e-11 64.3
MS.gene031499.t1 AT1G68670 50.725 69 29 1 3 71 206 269 3.99e-11 63.9
MS.gene031499.t1 AT1G25550 51.724 58 28 0 14 71 206 263 4.18e-11 63.9
MS.gene031499.t1 AT1G49190 51.724 58 25 1 17 71 215 272 9.61e-11 63.2

Find 57 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGAAATATTATTATAATT+TGG 0.167579 4.2:-27877353 None:intergenic
CTTCTGGCTCAATGGCCATT+AGG 0.226035 4.2:-27877385 None:intergenic
TTCTGGCTCAATGGCCATTA+GGG 0.263854 4.2:-27877384 None:intergenic
ATTACTCAGTGGTTTGGTTC+TGG 0.295890 4.2:-27876743 None:intergenic
GTTTCTGTTAGTTCTGAAAT+TGG 0.299096 4.2:-27877194 None:intergenic
AGTGTTCTCAGCCTCATTTA+TGG 0.303454 4.2:-27877330 None:intergenic
CCATCTTAAAAGTCATTTAC+AGG 0.331529 4.2:+27876451 MS.gene031499:CDS
AGCCAGAAGGAAGGTCATTC+AGG 0.335489 4.2:+27877399 MS.gene031499:CDS
TTCAGTTGAATTGCCCCTAA+TGG 0.340722 4.2:+27877370 MS.gene031499:CDS
TAATTGCATCAGTGAATCTT+TGG 0.343412 4.2:-27876255 None:intergenic
GTCCTGAATGACCTTCCTTC+TGG 0.358063 4.2:-27877401 None:intergenic
ACCTTGTTTGTTGTCAGAAC+AGG 0.382827 4.2:-27876583 None:intergenic
ATCACCCTCATCAAACTCTT+TGG 0.386936 4.2:-27876404 None:intergenic
TTAACTATTACTCAGTGGTT+TGG 0.396613 4.2:-27876749 None:intergenic
ATGAGGGTGATGGGAATTCC+AGG 0.406362 4.2:+27876416 MS.gene031499:CDS
AAAGAACCAAACGCATGCTT+TGG 0.416759 4.2:-27876189 None:intergenic
ATGCTAAGCCTAGGTTGAAA+TGG 0.427329 4.2:+27876218 MS.gene031499:CDS
AAGGCGCAGGAAGCACTTGC+AGG 0.427854 4.2:+27877074 MS.gene031499:CDS
TCTTCTTCTTCTTCGACAAC+GGG 0.438944 4.2:+27877104 MS.gene031499:CDS
GCAAAACCAAAGCATGCGTT+TGG 0.448457 4.2:+27876183 MS.gene031499:CDS
TGACCTTCCTTCTGGCTCAA+TGG 0.470884 4.2:-27877393 None:intergenic
AGTTCTGAAATTGGAGAACT+AGG 0.474659 4.2:-27877185 None:intergenic
GAAACTTTATGAACAAATTG+AGG 0.475081 4.2:+27876898 MS.gene031499:CDS
ACTGATGCAATTAATCAGCT+TGG 0.488534 4.2:+27876265 MS.gene031499:CDS
CAGAACAGGTTTCTACCTGT+TGG 0.489939 4.2:-27876569 None:intergenic
TAGACTTGGCAAAAGCCAAC+AGG 0.499299 4.2:+27876554 MS.gene031499:CDS
AATGCAAATGATGGAGGAAG+TGG 0.507541 4.2:+27877425 MS.gene031499:CDS
TGAGGCTCTTGAAGTACAAA+TGG 0.509949 4.2:+27876865 MS.gene031499:CDS
TTCAGGACTAATGCAAATGA+TGG 0.510398 4.2:+27877416 MS.gene031499:CDS
TCATGAGTCCATTTCAACCT+AGG 0.511636 4.2:-27876226 None:intergenic
AGATGGTACAAAGTGAGTCC+TGG 0.514393 4.2:-27876434 None:intergenic
CTTACAATCGGTACTGATGA+AGG 0.516794 4.2:+27877055 MS.gene031499:CDS
TTTCCACTGATGCTAAGCCT+AGG 0.525101 4.2:+27876209 MS.gene031499:CDS
AAGAGTTTGATGAGGGTGAT+GGG 0.525589 4.2:+27876407 MS.gene031499:CDS
CAACCTAGGCTTAGCATCAG+TGG 0.529572 4.2:-27876212 None:intergenic
TGCAGTAGAGAAATCAGTGT+TGG 0.530962 4.2:+27876717 MS.gene031499:CDS
TCAAGGAAAGTACTTACAAT+CGG 0.534694 4.2:+27877043 MS.gene031499:CDS
TTAGTTTAAACTTTGGTGAG+AGG 0.537590 4.2:+27877225 MS.gene031499:CDS
AAAGAGTTTGATGAGGGTGA+TGG 0.538821 4.2:+27876406 MS.gene031499:CDS
CAGTTTAGAATTGAAGCTCA+AGG 0.543693 4.2:+27877026 MS.gene031499:CDS
TGAAAGCTCCGAGAGAAAAG+AGG 0.556243 4.2:+27877298 MS.gene031499:CDS
GATGCAATTAATCAGCTTGG+AGG 0.562956 4.2:+27876268 MS.gene031499:CDS
GGTGAGAGGAAACAAAATAG+AGG 0.572697 4.2:+27877239 MS.gene031499:CDS
TCAGAACTAACAGAAACAAG+AGG 0.597866 4.2:+27877200 MS.gene031499:CDS
ATAGAAATTCAAAACAGTGA+TGG 0.607108 4.2:+27876690 MS.gene031499:CDS
ATCGGTACTGATGAAGGCGC+AGG 0.610899 4.2:+27877061 MS.gene031499:CDS
TCTGGCTCAATGGCCATTAG+GGG 0.623024 4.2:-27877383 None:intergenic
ACCTGTTCTGACAACAAACA+AGG 0.624931 4.2:+27876582 MS.gene031499:CDS
TTATTACAGAAATATAGACT+TGG 0.629448 4.2:+27876540 MS.gene031499:intron
AGGACTAATGCAAATGATGG+AGG 0.631823 4.2:+27877419 MS.gene031499:CDS
AGATGTGTTGATCAACCAGA+TGG 0.635020 4.2:+27877482 MS.gene031499:CDS
AACACCAAAGAGTTTGATGA+GGG 0.637512 4.2:+27876400 MS.gene031499:CDS
ATGCAAATGATGGAGGAAGT+GGG 0.639201 4.2:+27877426 MS.gene031499:CDS
TAGAAGCTTGAAGATTGCTG+AGG 0.644476 4.2:+27876847 MS.gene031499:intron
CAACACCAAAGAGTTTGATG+AGG 0.646969 4.2:+27876399 MS.gene031499:CDS
TGGCCATTGAGCCAGAAGGA+AGG 0.652849 4.2:+27877390 MS.gene031499:CDS
CTAATGGCCATTGAGCCAGA+AGG 0.682174 4.2:+27877386 MS.gene031499:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTGAAATATTATTATAATT+TGG - chr4.2:27877356-27877375 None:intergenic 10.0%
!! TTATTACAGAAATATAGACT+TGG + chr4.2:27876540-27876559 MS.gene031499:intron 20.0%
!!! TTTTTCACACTATATATATG+TGG + chr4.2:27876370-27876389 MS.gene031499:intron 20.0%
! ACAAATTAACTATTACTCAG+TGG - chr4.2:27876757-27876776 None:intergenic 25.0%
! ATAGAAATTCAAAACAGTGA+TGG + chr4.2:27876690-27876709 MS.gene031499:CDS 25.0%
! ATGATTAAGGACATGTTAAT+CGG + chr4.2:27876970-27876989 MS.gene031499:intron 25.0%
! GAAACTTTATGAACAAATTG+AGG + chr4.2:27876898-27876917 MS.gene031499:CDS 25.0%
!!! AATCTTGAGTTATTGATTCA+TGG + chr4.2:27876657-27876676 MS.gene031499:intron 25.0%
!!! AGAGGTTTTAGTTTAAACTT+TGG + chr4.2:27877218-27877237 MS.gene031499:CDS 25.0%
!!! TTTGAGCTTATTTGATTTTC+TGG + chr4.2:27876517-27876536 MS.gene031499:intron 25.0%
AAATATGTGGCAAAAAACTG+AGG + chr4.2:27877507-27877526 MS.gene031499:CDS 30.0%
ATCAATACTCATTCCTATCT+AGG + chr4.2:27876938-27876957 MS.gene031499:intron 30.0%
CCATCTTAAAAGTCATTTAC+AGG + chr4.2:27876451-27876470 MS.gene031499:CDS 30.0%
GGAAAAACAAACCATAAATG+AGG + chr4.2:27877319-27877338 MS.gene031499:CDS 30.0%
TAATTGCATCAGTGAATCTT+TGG - chr4.2:27876258-27876277 None:intergenic 30.0%
TATGACAGTAATAGAATGAC+TGG + chr4.2:27876615-27876634 MS.gene031499:intron 30.0%
TTAACTATTACTCAGTGGTT+TGG - chr4.2:27876752-27876771 None:intergenic 30.0%
TTAGTTTAAACTTTGGTGAG+AGG + chr4.2:27877225-27877244 MS.gene031499:CDS 30.0%
! GCTCAAACTCTTATAGTTTT+TGG - chr4.2:27876504-27876523 None:intergenic 30.0%
! TAGGTTTCAAGTGATGATTA+AGG + chr4.2:27876957-27876976 MS.gene031499:intron 30.0%
!! CCTGTAAATGACTTTTAAGA+TGG - chr4.2:27876454-27876473 None:intergenic 30.0%
!! GTTTCTGTTAGTTCTGAAAT+TGG - chr4.2:27877197-27877216 None:intergenic 30.0%
!! TCAAGGAAAGTACTTACAAT+CGG + chr4.2:27877043-27877062 MS.gene031499:CDS 30.0%
!! TTGTTTTTCCTCTTTTCTCT+CGG - chr4.2:27877309-27877328 None:intergenic 30.0%
!!! AGTGCAACTATTGATTTTGA+TGG + chr4.2:27877458-27877477 MS.gene031499:CDS 30.0%
AACACCAAAGAGTTTGATGA+GGG + chr4.2:27876400-27876419 MS.gene031499:CDS 35.0%
ACACTATATATATGTGGCAG+AGG + chr4.2:27876376-27876395 MS.gene031499:intron 35.0%
ACTGATGCAATTAATCAGCT+TGG + chr4.2:27876265-27876284 MS.gene031499:CDS 35.0%
CAACCAGATGGAAAAATATG+TGG + chr4.2:27877494-27877513 MS.gene031499:CDS 35.0%
TCAGAACTAACAGAAACAAG+AGG + chr4.2:27877200-27877219 MS.gene031499:CDS 35.0%
TCATCACTTGAAACCTAGAT+AGG - chr4.2:27876954-27876973 None:intergenic 35.0%
TGGCAAAAAACTGAGGAAAT+CGG + chr4.2:27877514-27877533 MS.gene031499:CDS 35.0%
TTCAGGACTAATGCAAATGA+TGG + chr4.2:27877416-27877435 MS.gene031499:CDS 35.0%
TTCTTCTTCTTCTTCGACAA+CGG + chr4.2:27877103-27877122 MS.gene031499:CDS 35.0%
! CAGTTTAGAATTGAAGCTCA+AGG + chr4.2:27877026-27877045 MS.gene031499:CDS 35.0%
! TCGGTTTGAAACATGATTTC+AGG + chr4.2:27876989-27877008 MS.gene031499:intron 35.0%
! TGACTGGTAGTTTTCTGATT+TGG + chr4.2:27876631-27876650 MS.gene031499:intron 35.0%
! TTGCCACATATTTTTCCATC+TGG - chr4.2:27877500-27877519 None:intergenic 35.0%
!! AGTTCTGAAATTGGAGAACT+AGG - chr4.2:27877188-27877207 None:intergenic 35.0%
AAAGAACCAAACGCATGCTT+TGG - chr4.2:27876192-27876211 None:intergenic 40.0%
AAAGAGTTTGATGAGGGTGA+TGG + chr4.2:27876406-27876425 MS.gene031499:CDS 40.0%
AAGAGTTTGATGAGGGTGAT+GGG + chr4.2:27876407-27876426 MS.gene031499:CDS 40.0%
ACCTGTTCTGACAACAAACA+AGG + chr4.2:27876582-27876601 MS.gene031499:CDS 40.0%
AGGACTAATGCAAATGATGG+AGG + chr4.2:27877419-27877438 MS.gene031499:CDS 40.0%
AGTGTTCTCAGCCTCATTTA+TGG - chr4.2:27877333-27877352 None:intergenic 40.0%
ATCACCCTCATCAAACTCTT+TGG - chr4.2:27876407-27876426 None:intergenic 40.0%
CAACACCAAAGAGTTTGATG+AGG + chr4.2:27876399-27876418 MS.gene031499:CDS 40.0%
GATGCAATTAATCAGCTTGG+AGG + chr4.2:27876268-27876287 MS.gene031499:CDS 40.0%
GGTGAGAGGAAACAAAATAG+AGG + chr4.2:27877239-27877258 MS.gene031499:CDS 40.0%
TAGAAGCTTGAAGATTGCTG+AGG + chr4.2:27876847-27876866 MS.gene031499:intron 40.0%
TCATGAGTCCATTTCAACCT+AGG - chr4.2:27876229-27876248 None:intergenic 40.0%
TCTTCTTCTTCTTCGACAAC+GGG + chr4.2:27877104-27877123 MS.gene031499:CDS 40.0%
TGAGGCTCTTGAAGTACAAA+TGG + chr4.2:27876865-27876884 MS.gene031499:CDS 40.0%
TGCAGTAGAGAAATCAGTGT+TGG + chr4.2:27876717-27876736 MS.gene031499:CDS 40.0%
TTCAGTTGAATTGCCCCTAA+TGG + chr4.2:27877370-27877389 MS.gene031499:CDS 40.0%
! AATGCAAATGATGGAGGAAG+TGG + chr4.2:27877425-27877444 MS.gene031499:CDS 40.0%
! AGATGTGTTGATCAACCAGA+TGG + chr4.2:27877482-27877501 MS.gene031499:CDS 40.0%
! ATGCAAATGATGGAGGAAGT+GGG + chr4.2:27877426-27877445 MS.gene031499:CDS 40.0%
! ATGCTAAGCCTAGGTTGAAA+TGG + chr4.2:27876218-27876237 MS.gene031499:CDS 40.0%
!! ACCTTGTTTGTTGTCAGAAC+AGG - chr4.2:27876586-27876605 None:intergenic 40.0%
!! ATTACTCAGTGGTTTGGTTC+TGG - chr4.2:27876746-27876765 None:intergenic 40.0%
!! CTTACAATCGGTACTGATGA+AGG + chr4.2:27877055-27877074 MS.gene031499:CDS 40.0%
AGATGGTACAAAGTGAGTCC+TGG - chr4.2:27876437-27876456 None:intergenic 45.0%
CAGAACAGGTTTCTACCTGT+TGG - chr4.2:27876572-27876591 None:intergenic 45.0%
GCAAAACCAAAGCATGCGTT+TGG + chr4.2:27876183-27876202 MS.gene031499:CDS 45.0%
TAGACTTGGCAAAAGCCAAC+AGG + chr4.2:27876554-27876573 MS.gene031499:CDS 45.0%
TGAAAGCTCCGAGAGAAAAG+AGG + chr4.2:27877298-27877317 MS.gene031499:CDS 45.0%
TTTCCACTGATGCTAAGCCT+AGG + chr4.2:27876209-27876228 MS.gene031499:CDS 45.0%
!! TTCTGGCTCAATGGCCATTA+GGG - chr4.2:27877387-27877406 None:intergenic 45.0%
AGCCAGAAGGAAGGTCATTC+AGG + chr4.2:27877399-27877418 MS.gene031499:CDS 50.0%
CAACCTAGGCTTAGCATCAG+TGG - chr4.2:27876215-27876234 None:intergenic 50.0%
CTAATGGCCATTGAGCCAGA+AGG + chr4.2:27877386-27877405 MS.gene031499:CDS 50.0%
GTCCTGAATGACCTTCCTTC+TGG - chr4.2:27877404-27877423 None:intergenic 50.0%
! TCTGGCTCAATGGCCATTAG+GGG - chr4.2:27877386-27877405 None:intergenic 50.0%
!! ATGAGGGTGATGGGAATTCC+AGG + chr4.2:27876416-27876435 MS.gene031499:CDS 50.0%
!! CTTCTGGCTCAATGGCCATT+AGG - chr4.2:27877388-27877407 None:intergenic 50.0%
!! TGACCTTCCTTCTGGCTCAA+TGG - chr4.2:27877396-27877415 None:intergenic 50.0%
TGGCCATTGAGCCAGAAGGA+AGG + chr4.2:27877390-27877409 MS.gene031499:CDS 55.0%
!! ATCGGTACTGATGAAGGCGC+AGG + chr4.2:27877061-27877080 MS.gene031499:CDS 55.0%
!! AAGGCGCAGGAAGCACTTGC+AGG + chr4.2:27877074-27877093 MS.gene031499:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 27876175 27877631 27876175 ID=MS.gene031499
chr4.2 mRNA 27876175 27877631 27876175 ID=MS.gene031499.t1;Parent=MS.gene031499
chr4.2 exon 27876175 27876298 27876175 ID=MS.gene031499.t1.exon1;Parent=MS.gene031499.t1
chr4.2 CDS 27876175 27876298 27876175 ID=cds.MS.gene031499.t1;Parent=MS.gene031499.t1
chr4.2 exon 27876396 27876472 27876396 ID=MS.gene031499.t1.exon2;Parent=MS.gene031499.t1
chr4.2 CDS 27876396 27876472 27876396 ID=cds.MS.gene031499.t1;Parent=MS.gene031499.t1
chr4.2 exon 27876549 27876603 27876549 ID=MS.gene031499.t1.exon3;Parent=MS.gene031499.t1
chr4.2 CDS 27876549 27876603 27876549 ID=cds.MS.gene031499.t1;Parent=MS.gene031499.t1
chr4.2 exon 27876685 27876760 27876685 ID=MS.gene031499.t1.exon4;Parent=MS.gene031499.t1
chr4.2 CDS 27876685 27876760 27876685 ID=cds.MS.gene031499.t1;Parent=MS.gene031499.t1
chr4.2 exon 27876850 27876919 27876850 ID=MS.gene031499.t1.exon5;Parent=MS.gene031499.t1
chr4.2 CDS 27876850 27876919 27876850 ID=cds.MS.gene031499.t1;Parent=MS.gene031499.t1
chr4.2 exon 27877011 27877631 27877011 ID=MS.gene031499.t1.exon6;Parent=MS.gene031499.t1
chr4.2 CDS 27877011 27877631 27877011 ID=cds.MS.gene031499.t1;Parent=MS.gene031499.t1
Gene Sequence

>MS.gene031499

ATGGATCAGCAAAACCAAAGCATGCGTTTGGTTCTTTCCACTGATGCTAAGCCTAGGTTGAAATGGACTCATGAACTTCACCAAAGATTCACTGATGCAATTAATCAGCTTGGAGGTGCAGAAAGTAAGTGTGACACAAAAAACTTACTATTTTTAGATCATAGTTTATATCATTATCTTAAGTACTAAAATGTATTTTTCACACTATATATATGTGGCAGAGGCAACACCAAAGAGTTTGATGAGGGTGATGGGAATTCCAGGACTCACTTTGTACCATCTTAAAAGTCATTTACAGGTATCATAATCTTTTTGCTACACTGAATCCAAAAACTATAAGAGTTTGAGCTTATTTGATTTTCTGGTTATTACAGAAATATAGACTTGGCAAAAGCCAACAGGTAGAAACCTGTTCTGACAACAAACAAGGTGAGTTACTGTATGACAGTAATAGAATGACTGGTAGTTTTCTGATTTGGCATAATCTTGAGTTATTGATTCATGGTGCAGATTACATAGAAATTCAAAACAGTGATGGTCAATGCAGTAGAGAAATCAGTGTTGGAAACCAGAACCAAACCACTGAGTAATAGTTAATTTGTTACTGAGCAAATATTTGAGCATCTTTTGTTGATGATTGAAGTATTTATTGATCAAATGTTTTGTGTTTACTAGAAGCTTGAAGATTGCTGAGGCTCTTGAAGTACAAATGGAAGTACAAAAGAAACTTTATGAACAAATTGAGGTAACATTACAAATTATGATCAATACTCATTCCTATCTAGGTTTCAAGTGATGATTAAGGACATGTTAATCGGTTTGAAACATGATTTCAGGTGCAGAAACATTTACAGTTTAGAATTGAAGCTCAAGGAAAGTACTTACAATCGGTACTGATGAAGGCGCAGGAAGCACTTGCAGGATATAGTTCTTCTTCTTCTTCGACAACGGGTGTAGAACATGCTAAAGCTGAACTTTCTCAGTTATTATCAATAATCAACAATGCATGTCCTAGTTCTCCAATTTCAGAACTAACAGAAACAAGAGGTTTTAGTTTAAACTTTGGTGAGAGGAAACAAAATAGAGGAACTATGTGTTCTTTAGAAAGTTCTCTTACATCGTCTGAAAGCTCCGAGAGAAAAGAGGAAAAACAAACCATAAATGAGGCTGAGAACACTCCAAATTATAATAATATTTCAGTTGAATTGCCCCTAATGGCCATTGAGCCAGAAGGAAGGTCATTCAGGACTAATGCAAATGATGGAGGAAGTGGGAGAAAGAGAAGTGCAACTATTGATTTTGATGGTAGATGTGTTGATCAACCAGATGGAAAAATATGTGGCAAAAAACTGAGGAAATCGGAGTTTTCACAAATGCTTGACTTGAACAGCAAGTATGAGAGAGACATTGACTCAAGTTCATTAGAAATAGATTTAAATTGCAGTTCAAGCTTTTAG
Protein sequence

>MS.gene031499.t1

MDQQNQSMRLVLSTDAKPRLKWTHELHQRFTDAINQLGGAEKATPKSLMRVMGIPGLTLYHLKSHLQKYRLGKSQQVETCSDNKQDYIEIQNSDGQCSREISVGNQNQTTESLKIAEALEVQMEVQKKLYEQIEVQKHLQFRIEAQGKYLQSVLMKAQEALAGYSSSSSSTTGVEHAKAELSQLLSIINNACPSSPISELTETRGFSLNFGERKQNRGTMCSLESSLTSSESSERKEEKQTINEAENTPNYNNISVELPLMAIEPEGRSFRTNANDGGSGRKRSATIDFDGRCVDQPDGKICGKKLRKSEFSQMLDLNSKYERDIDSSSLEIDLNCSSSF