AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031680.t1 MTR_4g078810 92.506 427 26 4 1 424 1 424 0.0 713
MS.gene031680.t1 MTR_2g090675 61.937 444 124 19 1 421 1 422 1.58e-155 446
MS.gene031680.t1 MTR_4g098870 35.789 285 140 9 145 421 299 548 1.72e-43 160
MS.gene031680.t1 MTR_4g098870 35.789 285 140 9 145 421 319 568 2.04e-43 160
MS.gene031680.t1 MTR_4g098870 35.789 285 140 9 145 421 338 587 2.81e-43 160
MS.gene031680.t1 MTR_5g014040 34.304 309 145 10 121 415 267 531 3.41e-42 156
MS.gene031680.t1 MTR_5g014040 33.758 314 145 11 121 415 227 496 4.21e-40 150
MS.gene031680.t1 MTR_5g014040 33.758 314 145 11 121 415 267 536 5.52e-40 150
MS.gene031680.t1 MTR_4g098870 38.776 147 77 3 137 275 291 432 2.67e-26 111
MS.gene031680.t1 MTR_4g098870 38.776 147 77 3 137 275 311 452 3.34e-26 111
MS.gene031680.t1 MTR_1g032570 30.808 198 116 5 81 277 68 245 1.29e-18 88.6
MS.gene031680.t1 MTR_1g032570 30.808 198 116 5 81 277 137 314 1.56e-18 88.2
MS.gene031680.t1 MTR_4g021760 39.785 93 55 1 120 212 134 225 3.08e-18 85.5
MS.gene031680.t1 MTR_3g106220 48.684 76 39 0 148 223 190 265 8.10e-18 86.3
MS.gene031680.t1 MTR_1g013170 56.061 66 29 0 153 218 188 253 1.04e-16 82.4
MS.gene031680.t1 MTR_7g117705 37.500 120 60 3 151 255 186 305 1.20e-16 82.0
MS.gene031680.t1 MTR_2g450070 54.839 62 28 0 151 212 208 269 4.44e-16 80.9
MS.gene031680.t1 MTR_2g450070 54.839 62 28 0 151 212 208 269 4.46e-16 80.9
MS.gene031680.t1 MTR_4g121020 56.667 60 26 0 155 214 110 169 6.65e-16 80.1
MS.gene031680.t1 MTR_4g121020 56.667 60 26 0 155 214 214 273 8.27e-16 80.1
MS.gene031680.t1 MTR_4g121020 56.667 60 26 0 155 214 214 273 8.50e-16 80.1
MS.gene031680.t1 MTR_7g026400 57.812 64 27 0 145 208 146 209 1.17e-15 79.3
MS.gene031680.t1 MTR_1g013180 56.667 60 26 0 153 212 339 398 2.48e-15 78.6
MS.gene031680.t1 MTR_4g021790 39.785 93 55 1 120 212 133 224 3.31e-15 75.9
MS.gene031680.t1 MTR_2g034960 40.230 87 47 1 155 236 210 296 1.07e-14 76.6
MS.gene031680.t1 MTR_7g089010 44.872 78 37 1 137 214 78 149 1.23e-14 74.7
MS.gene031680.t1 MTR_4g021855 41.573 89 51 1 124 212 137 224 4.43e-14 72.8
MS.gene031680.t1 MTR_8g079940 47.826 69 36 0 155 223 208 276 5.31e-14 74.3
MS.gene031680.t1 MTR_4g086835 42.045 88 47 1 152 239 270 353 6.15e-14 73.6
MS.gene031680.t1 MTR_4g064730 39.506 81 49 0 143 223 118 198 6.18e-14 72.8
MS.gene031680.t1 MTR_3g102600 50.000 54 27 0 161 214 88 141 9.55e-14 69.7
MS.gene031680.t1 MTR_4g113140 36.000 125 60 4 108 212 168 292 1.48e-13 72.4
MS.gene031680.t1 MTR_3g102600 50.000 54 27 0 161 214 137 190 2.03e-13 69.7
MS.gene031680.t1 MTR_4g021845 37.805 82 50 1 124 205 137 217 2.84e-13 69.3
MS.gene031680.t1 MTR_3g086100 43.662 71 40 0 153 223 125 195 3.39e-13 71.6
MS.gene031680.t1 MTR_3g102590 51.786 56 27 0 153 208 140 195 3.98e-13 69.3
MS.gene031680.t1 MTR_3g086100 43.662 71 40 0 153 223 196 266 5.46e-13 71.2
MS.gene031680.t1 MTR_4g131600 41.176 85 48 1 153 235 198 282 8.43e-13 70.5
MS.gene031680.t1 MTR_4g086835 41.573 89 47 2 152 239 270 354 1.91e-12 68.9
MS.gene031680.t1 MTR_4g131570 48.214 56 29 0 153 208 200 255 3.39e-12 68.6
MS.gene031680.t1 MTR_3g102600 48.936 47 24 0 161 207 88 134 3.88e-12 63.9
MS.gene031680.t1 MTR_5g041350 45.333 75 35 3 158 231 17 86 7.39e-12 66.6
MS.gene031680.t1 MTR_1g093080 36.275 102 52 2 147 235 202 303 8.61e-12 66.6
MS.gene031680.t1 MTR_1g053830 49.057 53 26 1 157 208 19 71 9.78e-12 61.6
MS.gene031680.t1 MTR_0450s0040 51.852 54 26 0 155 208 168 221 1.31e-11 65.9
MS.gene031680.t1 MTR_4g131580 48.214 56 29 0 153 208 197 252 1.98e-11 66.2
MS.gene031680.t1 MTR_3g102600 48.936 47 24 0 161 207 137 183 2.50e-11 62.8
MS.gene031680.t1 MTR_6g032990 54.545 55 24 1 158 211 44 98 2.71e-11 65.5
MS.gene031680.t1 MTR_1g080330 46.377 69 34 2 144 211 165 231 3.97e-11 63.9
MS.gene031680.t1 MTR_1g080330 46.377 69 34 2 144 211 165 231 5.42e-11 64.3
MS.gene031680.t1 MTR_5g017980 51.786 56 27 0 155 210 192 247 5.94e-11 63.9
MS.gene031680.t1 MTR_5g017980 51.786 56 27 0 155 210 191 246 6.06e-11 63.9
MS.gene031680.t1 MTR_3g102590 41.270 63 37 0 143 205 130 192 9.10e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031680.t1 AT2G20570 45.714 455 179 21 1 423 1 419 3.83e-82 259
MS.gene031680.t1 AT2G20570 44.161 471 179 22 1 423 1 435 4.63e-78 249
MS.gene031680.t1 AT5G44190 41.573 445 174 16 1 420 1 384 8.08e-73 233
MS.gene031680.t1 AT5G44190 43.686 293 121 10 1 277 1 265 1.39e-60 201
MS.gene031680.t1 AT2G20570 36.645 453 176 21 1 423 1 372 1.45e-40 148
MS.gene031680.t1 AT4G18020 32.266 406 186 12 37 423 191 526 1.18e-35 138
MS.gene031680.t1 AT4G18020 32.266 406 186 12 37 423 191 526 1.18e-35 138
MS.gene031680.t1 AT4G18020 32.266 406 186 12 37 423 191 526 1.18e-35 138
MS.gene031680.t1 AT4G18020 32.266 406 186 12 37 423 191 526 1.18e-35 138
MS.gene031680.t1 AT4G18020 32.266 406 186 12 37 423 191 526 1.18e-35 138
MS.gene031680.t1 AT4G18020 34.483 232 134 4 37 264 191 408 5.46e-30 121
MS.gene031680.t1 AT4G18020 34.483 232 134 4 37 264 191 408 5.46e-30 121
MS.gene031680.t1 AT4G18020 34.483 232 134 4 37 264 191 408 5.46e-30 121
MS.gene031680.t1 AT4G18020 34.483 232 134 4 37 264 191 408 5.46e-30 121
MS.gene031680.t1 AT4G18020 34.483 232 134 4 37 264 191 408 5.46e-30 121
MS.gene031680.t1 AT4G18020 34.483 232 134 4 37 264 191 408 5.46e-30 121
MS.gene031680.t1 AT5G58080 54.839 62 28 0 153 214 191 252 2.97e-16 81.3
MS.gene031680.t1 AT3G10760 41.772 79 46 0 136 214 85 163 2.59e-15 77.0
MS.gene031680.t1 AT2G01760 53.125 64 30 0 151 214 130 193 3.28e-15 76.6
MS.gene031680.t1 AT3G16857 39.815 108 56 2 107 214 127 225 3.94e-15 77.8
MS.gene031680.t1 AT2G25180 51.613 62 30 0 153 214 192 253 4.37e-15 77.8
MS.gene031680.t1 AT3G16857 39.815 108 56 2 107 214 197 295 5.60e-15 77.4
MS.gene031680.t1 AT3G16857 39.815 108 56 2 107 214 197 295 6.06e-15 77.4
MS.gene031680.t1 AT5G05090 51.667 60 29 0 155 214 80 139 6.50e-15 75.1
MS.gene031680.t1 AT4G31920 54.237 59 27 0 150 208 177 235 6.56e-15 77.0
MS.gene031680.t1 AT2G01760 53.125 64 30 0 151 214 195 258 7.04e-15 76.3
MS.gene031680.t1 AT5G59570 38.750 80 49 0 152 231 137 216 8.39e-15 75.1
MS.gene031680.t1 AT5G59570 38.750 80 49 0 152 231 137 216 8.39e-15 75.1
MS.gene031680.t1 AT2G01760 53.125 64 30 0 151 214 223 286 8.56e-15 76.3
MS.gene031680.t1 AT3G46640 46.479 71 36 1 155 223 143 213 2.21e-14 74.3
MS.gene031680.t1 AT3G46640 46.479 71 36 1 155 223 143 213 2.21e-14 74.3
MS.gene031680.t1 AT3G46640 46.479 71 36 1 155 223 143 213 4.58e-14 73.2
MS.gene031680.t1 AT2G40970 43.210 81 43 1 137 214 83 163 7.84e-14 71.6
MS.gene031680.t1 AT5G07210 47.945 73 34 1 152 224 218 286 9.26e-14 73.6
MS.gene031680.t1 AT5G07210 47.945 73 34 1 152 224 226 294 9.26e-14 73.6
MS.gene031680.t1 AT2G03500 39.423 104 62 1 148 250 225 328 1.22e-13 72.8
MS.gene031680.t1 AT1G13300 42.222 90 48 3 124 212 152 238 2.53e-12 68.2
MS.gene031680.t1 AT1G68670 37.037 108 63 2 134 236 193 300 5.27e-12 67.4
MS.gene031680.t1 AT2G27070 36.207 116 71 1 100 215 172 284 8.50e-12 67.4
MS.gene031680.t1 AT1G67710 33.884 121 70 2 104 214 67 187 1.89e-11 66.2
MS.gene031680.t1 AT3G04030 55.769 52 22 1 158 208 47 98 2.21e-11 65.5
MS.gene031680.t1 AT1G67710 33.884 121 70 2 104 214 58 178 2.25e-11 65.9
MS.gene031680.t1 AT3G04030 55.769 52 22 1 158 208 47 98 2.55e-11 65.5
MS.gene031680.t1 AT1G67710 33.884 121 70 2 104 214 132 252 3.41e-11 65.5
MS.gene031680.t1 AT5G18240 57.407 54 22 1 156 208 45 98 3.54e-11 65.1
MS.gene031680.t1 AT5G18240 57.407 54 22 1 156 208 45 98 3.54e-11 65.1
MS.gene031680.t1 AT1G25550 41.892 74 42 1 141 213 194 267 3.59e-11 64.7
MS.gene031680.t1 AT5G18240 57.407 54 22 1 156 208 45 98 3.89e-11 65.1
MS.gene031680.t1 AT5G18240 57.407 54 22 1 156 208 45 98 4.21e-11 64.7
MS.gene031680.t1 AT5G18240 57.407 54 22 1 156 208 45 98 4.21e-11 64.7
MS.gene031680.t1 AT4G28610 31.933 119 70 2 94 211 173 281 6.22e-11 64.3

Find 128 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGTGCCTTCAAGGATTT+TGG 0.156203 4.3:-30404843 MS.gene031680:CDS
CACCTCAAGATCCTTCATTT+TGG 0.206831 4.3:-30404271 MS.gene031680:CDS
CCTTTCCGAAGGAAATTTAT+TGG 0.220430 4.3:-30405956 MS.gene031680:CDS
TGGAGACATCTTACCGGATT+TGG 0.308487 4.3:-30405890 MS.gene031680:CDS
GGCCGGATCATTGCGTTTAT+CGG 0.317082 4.3:+30402273 None:intergenic
AAACCCATGGTGGGTGCAGC+TGG 0.319978 4.3:+30404446 None:intergenic
ATGGGGACATCCTTCAAATA+TGG 0.339407 4.3:-30404363 MS.gene031680:CDS
AACTTCGACGACTTCTTTGT+TGG 0.363264 4.3:-30405925 MS.gene031680:CDS
TGTGGTGGGATGCCGGGGAT+TGG 0.370513 4.3:+30401945 None:intergenic
ATACATGTAAGGGTCTAAAA+TGG 0.371409 4.3:+30404384 None:intergenic
ATGCCATGTGGTGGGATGCC+GGG 0.378024 4.3:+30401939 None:intergenic
ACAAATCTCCTGTGCAAGTC+TGG 0.392453 4.3:+30404896 None:intergenic
CCAGCACTTGATAGCGTAAT+GGG 0.402444 4.3:-30401486 MS.gene031680:CDS
GAGGATAAAACAAGCTGTTC+GGG 0.406204 4.3:-30405745 MS.gene031680:CDS
GAGGAGGAGGAGGATGAGGG+GGG 0.410471 4.3:+30404423 None:intergenic
TTAAAAGATGCAGGTGGAAA+AGG 0.411700 4.3:-30405664 MS.gene031680:CDS
GATGAATTCTTCGGCGTCAC+TGG 0.421305 4.3:-30405812 MS.gene031680:CDS
TGAGGATAAAACAAGCTGTT+CGG 0.423559 4.3:-30405746 MS.gene031680:CDS
GAGAGCATAGACGCAGCTAT+TGG 0.425120 4.3:-30401558 MS.gene031680:CDS
ATAATCTCCAAAATCCTTGA+AGG 0.430397 4.3:+30404836 None:intergenic
CTCCGATAAACGCAATGATC+CGG 0.431873 4.3:-30402275 MS.gene031680:CDS
CATCTTACCGGATTTGGTAA+TGG 0.436295 4.3:-30405884 MS.gene031680:CDS
TAACATTGCTAGTCACCTTC+AGG 0.436461 4.3:-30404789 MS.gene031680:intron
GAGAACTCAGCAAACATCTC+TGG 0.443524 4.3:+30405859 None:intergenic
ACCACTGTTGTCAGAGGTGG+TGG 0.450953 4.3:+30402234 None:intergenic
CATGCCATGTGGTGGGATGC+CGG 0.451155 4.3:+30401938 None:intergenic
TGGACAAGGCGGTGCCTTCA+AGG 0.452098 4.3:-30404850 MS.gene031680:CDS
TCTGGATCCATTACCAAATC+CGG 0.453183 4.3:+30405877 None:intergenic
ACAATTACAACGGGCGACTT+CGG 0.456230 4.3:-30405982 MS.gene031680:CDS
GGTGGGAAACAATGTGTTCC+TGG 0.458402 4.3:+30402252 None:intergenic
ATGGTGGGTGCAGCTGGCCA+TGG 0.458606 4.3:+30404452 None:intergenic
GCTTTCCAATAAATTTCCTT+CGG 0.459139 4.3:+30405951 None:intergenic
TCGAAGAAGTGACCGGGAGC+AGG 0.463586 4.3:+30401885 None:intergenic
CATGAGAGTGGCTGCGGTGT+AGG 0.464099 4.3:+30404317 None:intergenic
AATCAAAGGAGACAAGTATA+TGG 0.464153 4.3:-30404506 MS.gene031680:CDS
GAAGGTGACTAGCAATGTTA+TGG 0.464906 4.3:+30404792 None:intergenic
GAATTCAAAGAAAGATTATG+AGG 0.466750 4.3:-30405764 MS.gene031680:CDS
AAGCAGCAAGTTGGAATCAA+AGG 0.471928 4.3:-30404520 MS.gene031680:CDS
GCTGAGTTCTCCGTTAGTTA+CGG 0.472120 4.3:-30405847 MS.gene031680:CDS
ATGGCCAGCTGCACCCACCA+TGG 0.477260 4.3:-30404450 MS.gene031680:CDS
TGTTTATTGTGCAGGTGGAT+TGG 0.477271 4.3:-30404922 MS.gene031680:intron
AGACAAGTATATGGTGGTGT+AGG 0.478471 4.3:-30404497 MS.gene031680:CDS
GACAAGTATATGGTGGTGTA+GGG 0.478876 4.3:-30404496 MS.gene031680:CDS
TGGCCAGCTGCACCCACCAT+GGG 0.485571 4.3:-30404449 MS.gene031680:CDS
CCATGGTTGCCACTACCACT+TGG 0.489750 4.3:-30401519 MS.gene031680:CDS
AACATGCAGAGACCATCATT+TGG 0.489976 4.3:-30401972 MS.gene031680:intron
CGTCGATGGAGACATCTTAC+CGG 0.493741 4.3:-30405896 MS.gene031680:CDS
TCTTTGTTAGTGTTCCTCAA+TGG 0.497869 4.3:+30406048 None:intergenic
TCCAGCACTTGATAGCGTAA+TGG 0.499694 4.3:-30401487 MS.gene031680:CDS
ATTAGCACCCTTCCTGCTCC+CGG 0.500715 4.3:-30401897 MS.gene031680:CDS
GGGAGTACTACAATTACAAC+GGG 0.501212 4.3:-30405991 MS.gene031680:CDS
AGGATGTCCCCATACATGTA+AGG 0.503004 4.3:+30404373 None:intergenic
GAAGTGACCGGGAGCAGGAA+GGG 0.509953 4.3:+30401890 None:intergenic
TGCCATGTGGTGGGATGCCG+GGG 0.510146 4.3:+30401940 None:intergenic
CCAATAAATTTCCTTCGGAA+AGG 0.511123 4.3:+30405956 None:intergenic
TTGAGGAACACTAACAAAGA+TGG 0.514130 4.3:-30406045 MS.gene031680:CDS
CCCATTACGCTATCAAGTGC+TGG 0.514753 4.3:+30401486 None:intergenic
TGATTCCTCGCCGTAACTAA+CGG 0.519226 4.3:+30405837 None:intergenic
TTTGTTGGCATCAACGTCGA+TGG 0.522723 4.3:-30405910 MS.gene031680:CDS
GAAGGATCTTGAGGTGGTGG+AGG 0.522935 4.3:+30404278 None:intergenic
GGAATCAGAGATGAATTCTT+CGG 0.526908 4.3:-30405821 MS.gene031680:CDS
AATGAAGGATCTTGAGGTGG+TGG 0.528793 4.3:+30404275 None:intergenic
GCTTTGAGTCCAAGTGGTAG+TGG 0.528879 4.3:+30401510 None:intergenic
AGATGGAAACCAAGAAGAGA+TGG 0.529997 4.3:-30406028 MS.gene031680:CDS
AAATGGTGCAATGGTGCCAT+AGG 0.534447 4.3:+30404401 None:intergenic
AAAGACTGGTCCATATTTGA+AGG 0.536097 4.3:+30404353 None:intergenic
GGAAACAATGTGTTCCTGGC+CGG 0.538523 4.3:+30402256 None:intergenic
TTTCTTCAAGTGCATGATGA+GGG 0.538544 4.3:+30401429 None:intergenic
ATGGTGGTGTAGGGGTGAAG+AGG 0.540082 4.3:-30404487 MS.gene031680:CDS
TGGTGGTGTAGGGGTGAAGA+GGG 0.540938 4.3:-30404486 MS.gene031680:CDS
TGGGAAGTCGAAGAAGTGAC+CGG 0.545060 4.3:+30401878 None:intergenic
TGCAATGGTGCCATAGGAGG+AGG 0.545767 4.3:+30404407 None:intergenic
AAAACAAGCTGTTCGGGTCA+AGG 0.545941 4.3:-30405739 MS.gene031680:CDS
AATCCATTGTTAAAAGATGC+AGG 0.548676 4.3:-30405673 MS.gene031680:CDS
GGGATTGGACATCCAAATGA+TGG 0.551089 4.3:+30401960 None:intergenic
CTACACCGCAGCCACTCTCA+TGG 0.554231 4.3:-30404316 MS.gene031680:CDS
AAGCTGTTCGGGTCAAGGCT+CGG 0.557290 4.3:-30405734 MS.gene031680:CDS
TGGTGCAATGGTGCCATAGG+AGG 0.557595 4.3:+30404404 None:intergenic
GGTGCCATAGGAGGAGGAGG+AGG 0.561800 4.3:+30404413 None:intergenic
TTGGCATTCTCACCACCAGC+AGG 0.563830 4.3:-30404252 MS.gene031680:intron
CTTCAAGTGCATGATGAGGG+TGG 0.573822 4.3:+30401432 None:intergenic
TGCCAAAATGAAGGATCTTG+AGG 0.576157 4.3:+30404269 None:intergenic
TCATTTGGATGTCCAATCCC+CGG 0.576190 4.3:-30401957 MS.gene031680:CDS
GTGCAAGCTGTGGAACAGCT+AGG 0.587705 4.3:-30404875 MS.gene031680:CDS
GTGAAGCAGAAGCAGCAAGT+TGG 0.590428 4.3:-30404529 MS.gene031680:CDS
ATAAACGCAATGATCCGGCC+AGG 0.590506 4.3:-30402270 MS.gene031680:CDS
GGATGTCCCCATACATGTAA+GGG 0.590827 4.3:+30404374 None:intergenic
CCAAGTGGTAGTGGCAACCA+TGG 0.591902 4.3:+30401519 None:intergenic
AATGGTGCCATAGGAGGAGG+AGG 0.593332 4.3:+30404410 None:intergenic
GGTGTCCATGAGAGTGGCTG+CGG 0.595402 4.3:+30404311 None:intergenic
ATCCCCGGCATCCCACCACA+TGG 0.597645 4.3:-30401942 MS.gene031680:CDS
ATTGGACACCAGACTTGCAC+AGG 0.600253 4.3:-30404904 MS.gene031680:CDS
TTTAGACCCTTACATGTATG+GGG 0.601372 4.3:-30404380 MS.gene031680:CDS
TCACTTCTTCGACTTCCCAC+CGG 0.602135 4.3:-30401874 MS.gene031680:intron
GCTTTACACATGCCATGTGG+TGG 0.603962 4.3:+30401930 None:intergenic
GCCACTCTCATGGACACCAT+CGG 0.612134 4.3:-30404306 MS.gene031680:CDS
AGAAGTGACCGGGAGCAGGA+AGG 0.612201 4.3:+30401889 None:intergenic
GACTTCGGAGACCTTTCCGA+AGG 0.616050 4.3:-30405967 MS.gene031680:CDS
CTTTACACATGCCATGTGGT+GGG 0.616904 4.3:+30401931 None:intergenic
GCCACATGTGCAGAAAAGAC+TGG 0.621691 4.3:+30404339 None:intergenic
AGCTGTGGAACAGCTAGGAG+TGG 0.623334 4.3:-30404870 MS.gene031680:CDS
AGAGGGAAGTGATCAGTCCA+TGG 0.624850 4.3:-30404469 MS.gene031680:CDS
GGGAAGTCGAAGAAGTGACC+GGG 0.633060 4.3:+30401879 None:intergenic
TGCTTGCAGTGTCACCATTG+AGG 0.634204 4.3:-30406062 MS.gene031680:CDS
CAGGAGATTTGTGCAAGCTG+TGG 0.634615 4.3:-30404885 MS.gene031680:CDS
GGCATGTGTAAAGCAGATCA+CGG 0.636465 4.3:-30401921 MS.gene031680:CDS
ACAAGTATATGGTGGTGTAG+GGG 0.636855 4.3:-30404495 MS.gene031680:CDS
TGGTGAGAATGCCAAAATGA+AGG 0.637260 4.3:+30404260 None:intergenic
ACCGATGGTGTCCATGAGAG+TGG 0.639166 4.3:+30404305 None:intergenic
GTTACCACTGTTGTCAGAGG+TGG 0.641294 4.3:+30402231 None:intergenic
CAAAGGAGACAAGTATATGG+TGG 0.641945 4.3:-30404503 MS.gene031680:CDS
TCAAGCGAAGAGAAAAGTCA+AGG 0.644275 4.3:-30405611 MS.gene031680:intron
ATAGTTACCACTGTTGTCAG+AGG 0.658785 4.3:+30402228 None:intergenic
GGAACAGCTAGGAGTGGACA+AGG 0.659903 4.3:-30404864 MS.gene031680:CDS
TAGAGAGAGCTACCTGCTGG+TGG 0.667063 4.3:+30404240 None:intergenic
GGGGAGTACTACAATTACAA+CGG 0.686337 4.3:-30405992 MS.gene031680:CDS
GGTGGTGGAGGAGCTACCGA+TGG 0.688749 4.3:+30404290 None:intergenic
GAGTAAAAGAGATGAATCTG+TGG 0.689938 4.3:-30405701 MS.gene031680:CDS
GCTGGAGCTTTGAGTCCAAG+TGG 0.690127 4.3:+30401504 None:intergenic
AAGGGTCTAAAATGGTGCAA+TGG 0.690674 4.3:+30404392 None:intergenic
GTTCTCCGTTAGTTACGGCG+AGG 0.691247 4.3:-30405842 MS.gene031680:CDS
ATGGGTGAACTACAAAGACA+AGG 0.697859 4.3:-30401468 MS.gene031680:CDS
CCACTGTTGTCAGAGGTGGT+GGG 0.703169 4.3:+30402235 None:intergenic
CAAAATGAAGGATCTTGAGG+TGG 0.718294 4.3:+30404272 None:intergenic
CCCACCACCTCTGACAACAG+TGG 0.721504 4.3:-30402235 MS.gene031680:intron
TCTGCTTTACACATGCCATG+TGG 0.735223 4.3:+30401927 None:intergenic
CCATTGTTAAAAGATGCAGG+TGG 0.735818 4.3:-30405670 MS.gene031680:CDS
ACAGCTAGGAGTGGACAAGG+CGG 0.769326 4.3:-30404861 MS.gene031680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAACTAAATATCATT+TGG + chr4.3:30404609-30404628 None:intergenic 10.0%
!! AAAAAAACTAAATATCATTT+GGG + chr4.3:30404608-30404627 None:intergenic 10.0%
!! AAAAAAATTATATATGAGAT+CGG - chr4.3:30403725-30403744 MS.gene031680:intron 10.0%
!! AACCTTAAATAAATATTATT+TGG + chr4.3:30404127-30404146 None:intergenic 10.0%
!! ATATTCAACTATTAAATTAA+AGG - chr4.3:30404699-30404718 MS.gene031680:intron 10.0%
!! TTTGTCATATATTATATATA+AGG - chr4.3:30404799-30404818 MS.gene031680:CDS 10.0%
!!! CAAATTTTTTTTTTTTTTGA+GGG + chr4.3:30404947-30404966 None:intergenic 10.0%
!!! TCAAATTTTTTTTTTTTTTG+AGG + chr4.3:30404948-30404967 None:intergenic 10.0%
!!! TCTCTAATTTTTATTTTTTA+AGG - chr4.3:30404267-30404286 MS.gene031680:CDS 10.0%
!!! TCTCTTTTAATATTTTTTTT+GGG + chr4.3:30403840-30403859 None:intergenic 10.0%
!!! TTCTCTTTTAATATTTTTTT+TGG + chr4.3:30403841-30403860 None:intergenic 10.0%
!!! TTTTTATAGCATTTTTTTTT+CGG - chr4.3:30402243-30402262 MS.gene031680:CDS 10.0%
!! CAAATGGTATATATAAAAAT+TGG + chr4.3:30404332-30404351 None:intergenic 15.0%
!! CACATCAAATAAATATTATT+TGG + chr4.3:30404059-30404078 None:intergenic 15.0%
!! GTCCAAATAATATTTATTTA+AGG - chr4.3:30404122-30404141 MS.gene031680:intron 15.0%
!! TACTGCTATTATTATTAAAT+AGG - chr4.3:30402365-30402384 MS.gene031680:intron 15.0%
!! TATACAATGATAGATATATA+GGG + chr4.3:30403307-30403326 None:intergenic 15.0%
!! TTACTTATATATAAATTGTG+AGG + chr4.3:30402345-30402364 None:intergenic 15.0%
!!! ACAATTAAGATTTTAAACTT+TGG - chr4.3:30403367-30403386 MS.gene031680:intron 15.0%
!!! ATGTGAAACTTTTTAATATA+TGG + chr4.3:30405727-30405746 None:intergenic 15.0%
!!! CTAAACAATAATTTGTTTTT+GGG + chr4.3:30403635-30403654 None:intergenic 15.0%
!!! TAGGAAAATATACAATTTTT+AGG - chr4.3:30403761-30403780 MS.gene031680:intron 15.0%
!!! TTGTTTTAAAATCTTTTTCT+AGG + chr4.3:30404863-30404882 None:intergenic 15.0%
!! AGTACAAACTGAAATAATTA+AGG + chr4.3:30403404-30403423 None:intergenic 20.0%
!! ATATAAATTGTGAGGATATT+TGG + chr4.3:30402337-30402356 None:intergenic 20.0%
!! CCAAAAACAAATTATTGTTT+AGG - chr4.3:30403633-30403652 MS.gene031680:intron 20.0%
!! CTATACAATGATAGATATAT+AGG + chr4.3:30403308-30403327 None:intergenic 20.0%
!! GGTTTCATATATAAAAACAA+AGG - chr4.3:30403430-30403449 MS.gene031680:intron 20.0%
!! GTACAAACTGAAATAATTAA+GGG + chr4.3:30403403-30403422 None:intergenic 20.0%
!! TATTAAAAAAATGTTAGCAG+TGG - chr4.3:30402207-30402226 MS.gene031680:intron 20.0%
!! TGTGCTATATTTATATATGT+TGG + chr4.3:30405408-30405427 None:intergenic 20.0%
!! TGTTAACAATTCAAATTTAC+CGG - chr4.3:30402269-30402288 MS.gene031680:CDS 20.0%
!! TTATTCTCCAAATATAAGTT+TGG - chr4.3:30405479-30405498 MS.gene031680:intron 20.0%
!! TTGTTATAAATCAAAATTCG+AGG - chr4.3:30405068-30405087 MS.gene031680:intron 20.0%
!!! AATTCTCTTTATTTAGTTTC+CGG - chr4.3:30405701-30405720 MS.gene031680:CDS 20.0%
!!! ATTAAGTCACATCAATTTTT+TGG + chr4.3:30403998-30404017 None:intergenic 20.0%
!!! ATTACATCACTTTACTATTT+TGG - chr4.3:30405294-30405313 MS.gene031680:intron 20.0%
!!! CCTAAACAATAATTTGTTTT+TGG + chr4.3:30403636-30403655 None:intergenic 20.0%
!!! CTTTTAGGTGTTTTTATATT+GGG + chr4.3:30405834-30405853 None:intergenic 20.0%
!!! GAAAATATACAATTTTTAGG+AGG - chr4.3:30403764-30403783 MS.gene031680:intron 20.0%
!!! TGTCAATCTTATCTTTAAAA+AGG + chr4.3:30405356-30405375 None:intergenic 20.0%
!!! TGTTATGTTATTTTCCTTTA+TGG + chr4.3:30405509-30405528 None:intergenic 20.0%
!!! TTTGTCGACTTTTATATTTT+AGG - chr4.3:30404442-30404461 MS.gene031680:CDS 20.0%
!!! TTTTAGGTGTTTTTATATTG+GGG + chr4.3:30405833-30405852 None:intergenic 20.0%
!!! TTTTGAATATAACGAACAAA+TGG + chr4.3:30404348-30404367 None:intergenic 20.0%
!!! TTTTGTTATTGCAGAAATAT+AGG - chr4.3:30402920-30402939 MS.gene031680:intron 20.0%
! AACAAGGATTCAATTATATG+TGG + chr4.3:30404924-30404943 None:intergenic 25.0%
! AATTTGCATATTACATGTCA+TGG - chr4.3:30402454-30402473 MS.gene031680:intron 25.0%
! AGTCAAGGTATACTTAAATA+AGG - chr4.3:30401901-30401920 MS.gene031680:CDS 25.0%
! CATATGAATTTCAAGCATAT+AGG - chr4.3:30402733-30402752 MS.gene031680:intron 25.0%
! CTAAGTGGTTTATCTTAATT+TGG - chr4.3:30401946-30401965 MS.gene031680:CDS 25.0%
! GAATTCAAAGAAAGATTATG+AGG - chr4.3:30401733-30401752 MS.gene031680:intron 25.0%
! TGATAGATATATAGGGAATT+TGG + chr4.3:30403300-30403319 None:intergenic 25.0%
!! GAAACTTTTTAATATATGGC+CGG + chr4.3:30405723-30405742 None:intergenic 25.0%
!! GTTTTTATATATGAAACCAG+CGG + chr4.3:30403428-30403447 None:intergenic 25.0%
!! TGTGCTCAAATATTGTTATT+TGG + chr4.3:30403810-30403829 None:intergenic 25.0%
!! TTTTTATATATGAAACCAGC+GGG + chr4.3:30403427-30403446 None:intergenic 25.0%
!!! CACTATTTATCAAGTTTTGA+TGG - chr4.3:30402856-30402875 MS.gene031680:intron 25.0%
!!! GCTTTTAGGTGTTTTTATAT+TGG + chr4.3:30405835-30405854 None:intergenic 25.0%
!!! GTGCTCAAATATTGTTATTT+GGG + chr4.3:30403809-30403828 None:intergenic 25.0%
!!! TTTTTTTTTTTGAGGGAACA+AGG + chr4.3:30404940-30404959 None:intergenic 25.0%
AAATTAGAGAAAGTCTTTCG+TGG + chr4.3:30404154-30404173 None:intergenic 30.0%
AATCAAAGGAGACAAGTATA+TGG - chr4.3:30402991-30403010 MS.gene031680:intron 30.0%
AATCCATTGTTAAAAGATGC+AGG - chr4.3:30401824-30401843 MS.gene031680:intron 30.0%
AATTAGTGTGTTTATTGTGC+AGG - chr4.3:30402567-30402586 MS.gene031680:intron 30.0%
AGGATTCAATTATATGTGGT+TGG + chr4.3:30404920-30404939 None:intergenic 30.0%
ATAATCTCCAAAATCCTTGA+AGG + chr4.3:30402664-30402683 None:intergenic 30.0%
ATACATGTAAGGGTCTAAAA+TGG + chr4.3:30403116-30403135 None:intergenic 30.0%
CCTATTTACGATGAATATAC+CGG + chr4.3:30402291-30402310 None:intergenic 30.0%
GCTTTCCAATAAATTTCCTT+CGG + chr4.3:30401549-30401568 None:intergenic 30.0%
TAAAAGCTCCATAGATTTCA+AGG - chr4.3:30405848-30405867 MS.gene031680:CDS 30.0%
TGAAATTCATATGTACCTGA+AGG + chr4.3:30402726-30402745 None:intergenic 30.0%
TGAATGCATGTATGTATGTA+TGG + chr4.3:30405665-30405684 None:intergenic 30.0%
TTCAAGCATATAGGAGAAAA+AGG - chr4.3:30402742-30402761 MS.gene031680:intron 30.0%
TTTATAGTGCGTTGTATGTT+TGG - chr4.3:30402130-30402149 MS.gene031680:intron 30.0%
! ATTTTAGACCCTTACATGTA+TGG - chr4.3:30403115-30403134 MS.gene031680:intron 30.0%
! CTGTCTCTATAATTTGTCAA+GGG + chr4.3:30402055-30402074 None:intergenic 30.0%
! GAGATGTTTTATCAAAACCA+TGG - chr4.3:30405961-30405980 MS.gene031680:CDS 30.0%
! TCTGTCTCTATAATTTGTCA+AGG + chr4.3:30402056-30402075 None:intergenic 30.0%
! TTGAAATCTATGGAGCTTTT+AGG + chr4.3:30405849-30405868 None:intergenic 30.0%
! TTGTCTTCTACTCTGAATTT+TGG - chr4.3:30405328-30405347 MS.gene031680:intron 30.0%
! TTTTAGACCCTTACATGTAT+GGG - chr4.3:30403116-30403135 MS.gene031680:intron 30.0%
!! TTCAAGGATTTTGGAGATTA+TGG - chr4.3:30402663-30402682 MS.gene031680:intron 30.0%
!!! AAGTCACATCAATTTTTTGG+TGG + chr4.3:30403995-30404014 None:intergenic 30.0%
!!! AATTTTTTAGCATATGCCCT+TGG + chr4.3:30403474-30403493 None:intergenic 30.0%
!!! AGAGTTTTATTCTTGCTAAG+TGG - chr4.3:30401931-30401950 MS.gene031680:CDS 30.0%
!!! ATTTTTTAGCATATGCCCTT+GGG + chr4.3:30403473-30403492 None:intergenic 30.0%
!!! CAGTACTAACTTTTTAGCTA+AGG - chr4.3:30402004-30402023 MS.gene031680:intron 30.0%
AAAAACTCTCCATCTCTTCT+TGG + chr4.3:30401481-30401500 None:intergenic 35.0%
ACATTGTATGAGAGACGATA+AGG - chr4.3:30405743-30405762 MS.gene031680:CDS 35.0%
ATAAGTTTGGCTGACCATAA+AGG - chr4.3:30405492-30405511 MS.gene031680:intron 35.0%
CATTGTATGAGAGACGATAA+GGG - chr4.3:30405744-30405763 MS.gene031680:CDS 35.0%
CCAATAAATTTCCTTCGGAA+AGG + chr4.3:30401544-30401563 None:intergenic 35.0%
CCGGTATATTCATCGTAAAT+AGG - chr4.3:30402288-30402307 MS.gene031680:intron 35.0%
CCTTTCCGAAGGAAATTTAT+TGG - chr4.3:30401541-30401560 MS.gene031680:CDS 35.0%
GAGTAAAAGAGATGAATCTG+TGG - chr4.3:30401796-30401815 MS.gene031680:intron 35.0%
GGAATCAGAGATGAATTCTT+CGG - chr4.3:30401676-30401695 MS.gene031680:intron 35.0%
TACTCATGCCTTGAAATCTA+TGG + chr4.3:30405859-30405878 None:intergenic 35.0%
TCGATATGTACAAGTACGTT+TGG + chr4.3:30404725-30404744 None:intergenic 35.0%
TCTTTGTTAGTGTTCCTCAA+TGG + chr4.3:30401452-30401471 None:intergenic 35.0%
TGAGGATAAAACAAGCTGTT+CGG - chr4.3:30401751-30401770 MS.gene031680:intron 35.0%
TGGTCAGCCAAACTTATATT+TGG + chr4.3:30405489-30405508 None:intergenic 35.0%
TTAAAAGATGCAGGTGGAAA+AGG - chr4.3:30401833-30401852 MS.gene031680:intron 35.0%
TTGAGGAACACTAACAAAGA+TGG - chr4.3:30401452-30401471 MS.gene031680:CDS 35.0%
TTTAGACCCTTACATGTATG+GGG - chr4.3:30403117-30403136 MS.gene031680:intron 35.0%
TTTAGGAGGAACACTTACTT+GGG - chr4.3:30403778-30403797 MS.gene031680:intron 35.0%
! AAAGACTGGTCCATATTTGA+AGG + chr4.3:30403147-30403166 None:intergenic 35.0%
! GATAAGGGCATGTGTTTATA+AGG - chr4.3:30405759-30405778 MS.gene031680:CDS 35.0%
!! ATTCTTAACTTGTGCCCTAA+GGG - chr4.3:30403594-30403613 MS.gene031680:intron 35.0%
!! TTTTAGGAGGAACACTTACT+TGG - chr4.3:30403777-30403796 MS.gene031680:intron 35.0%
!!! AATTTGGCTGCTATTTTCAG+TGG + chr4.3:30403284-30403303 None:intergenic 35.0%
!!! ATCAATTTTTTGGTGGTGTC+CGG + chr4.3:30403988-30404007 None:intergenic 35.0%
!!! GTTCTTCTTCTTTTTTGACG+AGG + chr4.3:30404973-30404992 None:intergenic 35.0%
!!! TTTTTTAGCATATGCCCTTG+GGG + chr4.3:30403472-30403491 None:intergenic 35.0%
AACATGCAGAGACCATCATT+TGG - chr4.3:30405525-30405544 MS.gene031680:intron 40.0%
AACTTCGACGACTTCTTTGT+TGG - chr4.3:30401572-30401591 MS.gene031680:intron 40.0%
ACAACGCATGGTAATATGCA+AGG - chr4.3:30403924-30403943 MS.gene031680:intron 40.0%
AGATGGAAACCAAGAAGAGA+TGG - chr4.3:30401469-30401488 MS.gene031680:CDS 40.0%
AGGGCATGTGTTTATAAGGT+GGG - chr4.3:30405763-30405782 MS.gene031680:CDS 40.0%
ATAGTTACCACTGTTGTCAG+AGG + chr4.3:30405272-30405291 None:intergenic 40.0%
ATGGGGACATCCTTCAAATA+TGG - chr4.3:30403134-30403153 MS.gene031680:intron 40.0%
ATGGGTGAACTACAAAGACA+AGG - chr4.3:30406029-30406048 MS.gene031680:CDS 40.0%
ATGGTAATATGCAAGGTCCA+GGG - chr4.3:30403931-30403950 MS.gene031680:intron 40.0%
CAAAATGAAGGATCTTGAGG+TGG + chr4.3:30403228-30403247 None:intergenic 40.0%
CAAAGGAGACAAGTATATGG+TGG - chr4.3:30402994-30403013 MS.gene031680:intron 40.0%
CATGTATGTATGTATGGCGA+AGG + chr4.3:30405659-30405678 None:intergenic 40.0%
CCATTGTTAAAAGATGCAGG+TGG - chr4.3:30401827-30401846 MS.gene031680:intron 40.0%
GAAGGTGACTAGCAATGTTA+TGG + chr4.3:30402708-30402727 None:intergenic 40.0%
GAGGATAAAACAAGCTGTTC+GGG - chr4.3:30401752-30401771 MS.gene031680:intron 40.0%
GCAACTGTTCAAAATTTCGC+TGG - chr4.3:30402093-30402112 MS.gene031680:intron 40.0%
GTACTCGTACGATGTCTTTA+TGG - chr4.3:30402809-30402828 MS.gene031680:intron 40.0%
TAACATTGCTAGTCACCTTC+AGG - chr4.3:30402708-30402727 MS.gene031680:intron 40.0%
TAGGATCTTCATCTCTAACG+AGG - chr4.3:30402307-30402326 MS.gene031680:intron 40.0%
TAGTGTGTTTATTGTGCAGG+TGG - chr4.3:30402570-30402589 MS.gene031680:intron 40.0%
TCAAGCGAAGAGAAAAGTCA+AGG - chr4.3:30401886-30401905 MS.gene031680:CDS 40.0%
TCTGGATCCATTACCAAATC+CGG + chr4.3:30401623-30401642 None:intergenic 40.0%
TGCCAAAATGAAGGATCTTG+AGG + chr4.3:30403231-30403250 None:intergenic 40.0%
TGGTGAGAATGCCAAAATGA+AGG + chr4.3:30403240-30403259 None:intergenic 40.0%
TGTTTATTGTGCAGGTGGAT+TGG - chr4.3:30402575-30402594 MS.gene031680:intron 40.0%
! AAGCAGCAAGTTGGAATCAA+AGG - chr4.3:30402977-30402996 MS.gene031680:intron 40.0%
! AAGGGCATGTGTTTATAAGG+TGG - chr4.3:30405762-30405781 MS.gene031680:CDS 40.0%
! AAGGGTCTAAAATGGTGCAA+TGG + chr4.3:30403108-30403127 None:intergenic 40.0%
! ACAAGTATATGGTGGTGTAG+GGG - chr4.3:30403002-30403021 MS.gene031680:intron 40.0%
! AGACAAGTATATGGTGGTGT+AGG - chr4.3:30403000-30403019 MS.gene031680:intron 40.0%
! CACCTCAAGATCCTTCATTT+TGG - chr4.3:30403226-30403245 MS.gene031680:intron 40.0%
! CATCTTACCGGATTTGGTAA+TGG - chr4.3:30401613-30401632 MS.gene031680:intron 40.0%
! CCACCTGCATCTTTTAACAA+TGG + chr4.3:30401830-30401849 None:intergenic 40.0%
! CTTGTTATCTTGTGCCCTTA+GGG + chr4.3:30403611-30403630 None:intergenic 40.0%
! GACAAGTATATGGTGGTGTA+GGG - chr4.3:30403001-30403020 MS.gene031680:intron 40.0%
! TCTTGTTATCTTGTGCCCTT+AGG + chr4.3:30403612-30403631 None:intergenic 40.0%
! TGAACTTAGCTCAGTTGGTA+GGG - chr4.3:30403901-30403920 MS.gene031680:intron 40.0%
! TTGACTTTTCTCTTCGCTTG+AGG + chr4.3:30401887-30401906 None:intergenic 40.0%
!! AAATGTTAACTTGTGCCCCA+AGG - chr4.3:30403454-30403473 MS.gene031680:intron 40.0%
!! AATGTTAACTTGTGCCCCAA+GGG - chr4.3:30403455-30403474 MS.gene031680:intron 40.0%
!! AGAGATGGAGAGTTTTTCGA+TGG - chr4.3:30401484-30401503 MS.gene031680:CDS 40.0%
!! AGATGGAGAGTTTTTCGATG+GGG - chr4.3:30401486-30401505 MS.gene031680:CDS 40.0%
!! AGTGTCTCGTGCACTTTTTA+AGG + chr4.3:30402527-30402546 None:intergenic 40.0%
!! GAGATGGAGAGTTTTTCGAT+GGG - chr4.3:30401485-30401504 MS.gene031680:CDS 40.0%
!! GATCGGAGAGAGTATTTGAT+AGG - chr4.3:30403742-30403761 MS.gene031680:intron 40.0%
!! GATTCTTAACTTGTGCCCTA+AGG - chr4.3:30403593-30403612 MS.gene031680:intron 40.0%
!! GGGAGTACTACAATTACAAC+GGG - chr4.3:30401506-30401525 MS.gene031680:CDS 40.0%
!! GGGGAGTACTACAATTACAA+CGG - chr4.3:30401505-30401524 MS.gene031680:CDS 40.0%
!!! TCAATTTTTTGGTGGTGTCC+GGG + chr4.3:30403987-30404006 None:intergenic 40.0%
AAAACAAGCTGTTCGGGTCA+AGG - chr4.3:30401758-30401777 MS.gene031680:intron 45.0%
AATGAAGGATCTTGAGGTGG+TGG + chr4.3:30403225-30403244 None:intergenic 45.0%
ACAAATCTCCTGTGCAAGTC+TGG + chr4.3:30402604-30402623 None:intergenic 45.0%
ACAATTACAACGGGCGACTT+CGG - chr4.3:30401515-30401534 MS.gene031680:CDS 45.0%
AGAGTAGAAGACAACGTGCA+TGG + chr4.3:30405322-30405341 None:intergenic 45.0%
AGGATGTCCCCATACATGTA+AGG + chr4.3:30403127-30403146 None:intergenic 45.0%
ATTTCAGTTTGTACTCCCGC+TGG - chr4.3:30403409-30403428 MS.gene031680:intron 45.0%
CATGGTAATATGCAAGGTCC+AGG - chr4.3:30403930-30403949 MS.gene031680:intron 45.0%
CTCCGATAAACGCAATGATC+CGG - chr4.3:30405222-30405241 MS.gene031680:intron 45.0%
CTTTACACATGCCATGTGGT+GGG + chr4.3:30405569-30405588 None:intergenic 45.0%
GAGAACTCAGCAAACATCTC+TGG + chr4.3:30401641-30401660 None:intergenic 45.0%
GCTGAGTTCTCCGTTAGTTA+CGG - chr4.3:30401650-30401669 MS.gene031680:intron 45.0%
GGATGTCCCCATACATGTAA+GGG + chr4.3:30403126-30403145 None:intergenic 45.0%
GGCATGTGTAAAGCAGATCA+CGG - chr4.3:30405576-30405595 MS.gene031680:intron 45.0%
GGGATTGGACATCCAAATGA+TGG + chr4.3:30405540-30405559 None:intergenic 45.0%
GTGAACTTAGCTCAGTTGGT+AGG - chr4.3:30403900-30403919 MS.gene031680:intron 45.0%
TCATTTGGATGTCCAATCCC+CGG - chr4.3:30405540-30405559 MS.gene031680:intron 45.0%
TCTGCTTTACACATGCCATG+TGG + chr4.3:30405573-30405592 None:intergenic 45.0%
TGATTCCTCGCCGTAACTAA+CGG + chr4.3:30401663-30401682 None:intergenic 45.0%
TGGAGACATCTTACCGGATT+TGG - chr4.3:30401607-30401626 MS.gene031680:intron 45.0%
TGGCGAAGGAAATTCAGTAC+CGG + chr4.3:30405645-30405664 None:intergenic 45.0%
TTTGTTGGCATCAACGTCGA+TGG - chr4.3:30401587-30401606 MS.gene031680:intron 45.0%
! ACCAGTCTTTTCTGCACATG+TGG - chr4.3:30403157-30403176 MS.gene031680:intron 45.0%
!! AAATGGTGCAATGGTGCCAT+AGG + chr4.3:30403099-30403118 None:intergenic 45.0%
!! CCAGCACTTGATAGCGTAAT+GGG - chr4.3:30406011-30406030 MS.gene031680:CDS 45.0%
!! GAAGGAAATTCAGTACCGGT+GGG + chr4.3:30405641-30405660 None:intergenic 45.0%
!! TCCAGCACTTGATAGCGTAA+TGG - chr4.3:30406010-30406029 MS.gene031680:CDS 45.0%
!!! CAATTTTTTGGTGGTGTCCG+GGG + chr4.3:30403986-30404005 None:intergenic 45.0%
!!! TAATAATTAAGATTTTTTTT+TGG - chr4.3:30403334-30403353 MS.gene031680:intron 5.0%
AGAGGGAAGTGATCAGTCCA+TGG - chr4.3:30403028-30403047 MS.gene031680:intron 50.0%
ATAAACGCAATGATCCGGCC+AGG - chr4.3:30405227-30405246 MS.gene031680:intron 50.0%
ATTGGACACCAGACTTGCAC+AGG - chr4.3:30402593-30402612 MS.gene031680:intron 50.0%
CAGGAGATTTGTGCAAGCTG+TGG - chr4.3:30402612-30402631 MS.gene031680:intron 50.0%
CCAACTGAGCTAAGTTCACG+AGG + chr4.3:30403899-30403918 None:intergenic 50.0%
CCCATTACGCTATCAAGTGC+TGG + chr4.3:30406014-30406033 None:intergenic 50.0%
CCTCGTGAACTTAGCTCAGT+TGG - chr4.3:30403896-30403915 MS.gene031680:intron 50.0%
CGTCGATGGAGACATCTTAC+CGG - chr4.3:30401601-30401620 MS.gene031680:intron 50.0%
GATGAATTCTTCGGCGTCAC+TGG - chr4.3:30401685-30401704 MS.gene031680:intron 50.0%
GCCACATGTGCAGAAAAGAC+TGG + chr4.3:30403161-30403180 None:intergenic 50.0%
GCTTTACACATGCCATGTGG+TGG + chr4.3:30405570-30405589 None:intergenic 50.0%
GGAAACAATGTGTTCCTGGC+CGG + chr4.3:30405244-30405263 None:intergenic 50.0%
GGCCGGATCATTGCGTTTAT+CGG + chr4.3:30405227-30405246 None:intergenic 50.0%
GGTGGGAAACAATGTGTTCC+TGG + chr4.3:30405248-30405267 None:intergenic 50.0%
GTGAAGCAGAAGCAGCAAGT+TGG - chr4.3:30402968-30402987 MS.gene031680:intron 50.0%
GTTACCACTGTTGTCAGAGG+TGG + chr4.3:30405269-30405288 None:intergenic 50.0%
TCACTTCTTCGACTTCCCAC+CGG - chr4.3:30405623-30405642 MS.gene031680:CDS 50.0%
TGCTTGCAGTGTCACCATTG+AGG - chr4.3:30401435-30401454 MS.gene031680:CDS 50.0%
TGGGAAGTCGAAGAAGTGAC+CGG + chr4.3:30405622-30405641 None:intergenic 50.0%
! CGAAGGAAATTCAGTACCGG+TGG + chr4.3:30405642-30405661 None:intergenic 50.0%
! GAGAGCATAGACGCAGCTAT+TGG - chr4.3:30405939-30405958 MS.gene031680:CDS 50.0%
! GCTTTGAGTCCAAGTGGTAG+TGG + chr4.3:30405990-30406009 None:intergenic 50.0%
AAGCTGTTCGGGTCAAGGCT+CGG - chr4.3:30401763-30401782 MS.gene031680:intron 55.0%
AAGGTCCAGGGTTCAAACCC+CGG - chr4.3:30403943-30403962 MS.gene031680:intron 55.0%
AAGTGCACGAGACACTCGTC+AGG - chr4.3:30402531-30402550 MS.gene031680:intron 55.0%
ACAGCTAGGAGTGGACAAGG+CGG - chr4.3:30402636-30402655 MS.gene031680:intron 55.0%
ACCACTGTTGTCAGAGGTGG+TGG + chr4.3:30405266-30405285 None:intergenic 55.0%
ACCGATGGTGTCCATGAGAG+TGG + chr4.3:30403195-30403214 None:intergenic 55.0%
AGCTGTGGAACAGCTAGGAG+TGG - chr4.3:30402627-30402646 MS.gene031680:intron 55.0%
ATAGGAGGAGGAGGAGGATG+AGG + chr4.3:30403081-30403100 None:intergenic 55.0%
ATTAGCACCCTTCCTGCTCC+CGG - chr4.3:30405600-30405619 MS.gene031680:intron 55.0%
CCACTGTTGTCAGAGGTGGT+GGG + chr4.3:30405265-30405284 None:intergenic 55.0%
CCATGGTTGCCACTACCACT+TGG - chr4.3:30405978-30405997 MS.gene031680:CDS 55.0%
GACTTCGGAGACCTTTCCGA+AGG - chr4.3:30401530-30401549 MS.gene031680:CDS 55.0%
GCCACTCTCATGGACACCAT+CGG - chr4.3:30403191-30403210 MS.gene031680:intron 55.0%
GGAACAGCTAGGAGTGGACA+AGG - chr4.3:30402633-30402652 MS.gene031680:intron 55.0%
GGGAAGTCGAAGAAGTGACC+GGG + chr4.3:30405621-30405640 None:intergenic 55.0%
GGTTAGAGAGAGCTACCTGC+TGG + chr4.3:30403263-30403282 None:intergenic 55.0%
GGTTTGAACCCTGGACCTTG+CGG + chr4.3:30403942-30403961 None:intergenic 55.0%
GTGCAAGCTGTGGAACAGCT+AGG - chr4.3:30402622-30402641 MS.gene031680:intron 55.0%
GTTCTCCGTTAGTTACGGCG+AGG - chr4.3:30401655-30401674 MS.gene031680:intron 55.0%
GTTTGAACCCTGGACCTTGC+GGG + chr4.3:30403941-30403960 None:intergenic 55.0%
TAGAGAGAGCTACCTGCTGG+TGG + chr4.3:30403260-30403279 None:intergenic 55.0%
TAGGAGGAGGAGGAGGATGA+GGG + chr4.3:30403080-30403099 None:intergenic 55.0%
TCATCCTCCTCCTCCTCCTA+TGG - chr4.3:30403080-30403099 MS.gene031680:intron 55.0%
TTCAAACCCCGCAAGGTCCA+GGG - chr4.3:30403954-30403973 MS.gene031680:intron 55.0%
TTGGCATTCTCACCACCAGC+AGG - chr4.3:30403245-30403264 MS.gene031680:intron 55.0%
TTTGAACCCTGGACCTTGCG+GGG + chr4.3:30403963-30403982 None:intergenic 55.0%
! ATGGTGGTGTAGGGGTGAAG+AGG - chr4.3:30403010-30403029 MS.gene031680:intron 55.0%
! GAAGGATCTTGAGGTGGTGG+AGG + chr4.3:30403222-30403241 None:intergenic 55.0%
! GGCGGTGCCTTCAAGGATTT+TGG - chr4.3:30402654-30402673 MS.gene031680:intron 55.0%
! TGGTGGTGTAGGGGTGAAGA+GGG - chr4.3:30403011-30403030 MS.gene031680:intron 55.0%
!! AATGGTGCCATAGGAGGAGG+AGG + chr4.3:30403090-30403109 None:intergenic 55.0%
!! CAGTTGGTAGGGACAACGCA+TGG - chr4.3:30403912-30403931 MS.gene031680:intron 55.0%
!! CCAAGTGGTAGTGGCAACCA+TGG + chr4.3:30405981-30406000 None:intergenic 55.0%
!! GCTGGAGCTTTGAGTCCAAG+TGG + chr4.3:30405996-30406015 None:intergenic 55.0%
!! TGCAATGGTGCCATAGGAGG+AGG + chr4.3:30403093-30403112 None:intergenic 55.0%
!! TGGTGCAATGGTGCCATAGG+AGG + chr4.3:30403096-30403115 None:intergenic 55.0%
ACCTTGCGGGGTTTGAACCC+TGG + chr4.3:30403951-30403970 None:intergenic 60.0%
AGAAGTGACCGGGAGCAGGA+AGG + chr4.3:30405611-30405630 None:intergenic 60.0%
AGGAGGAGGAGGAGGATGAG+GGG + chr4.3:30403079-30403098 None:intergenic 60.0%
AGGATGAGGGGGGAAACCCA+TGG + chr4.3:30403067-30403086 None:intergenic 60.0%
ATGAGGGGGGAAACCCATGG+TGG + chr4.3:30403064-30403083 None:intergenic 60.0%
CATGAGAGTGGCTGCGGTGT+AGG + chr4.3:30403183-30403202 None:intergenic 60.0%
CCCACCACCTCTGACAACAG+TGG - chr4.3:30405262-30405281 MS.gene031680:intron 60.0%
CTACACCGCAGCCACTCTCA+TGG - chr4.3:30403181-30403200 MS.gene031680:intron 60.0%
GAAGTGACCGGGAGCAGGAA+GGG + chr4.3:30405610-30405629 None:intergenic 60.0%
GGTGTCCATGAGAGTGGCTG+CGG + chr4.3:30403189-30403208 None:intergenic 60.0%
GTTCAAACCCCGCAAGGTCC+AGG - chr4.3:30403953-30403972 MS.gene031680:intron 60.0%
TCCAGGGTTCAAACCCCGCA+AGG - chr4.3:30403947-30403966 MS.gene031680:intron 60.0%
TCGAAGAAGTGACCGGGAGC+AGG + chr4.3:30405615-30405634 None:intergenic 60.0%
TGAGGGGGGAAACCCATGGT+GGG + chr4.3:30403063-30403082 None:intergenic 60.0%
TGGACAAGGCGGTGCCTTCA+AGG - chr4.3:30402647-30402666 MS.gene031680:intron 60.0%
! ATGCCATGTGGTGGGATGCC+GGG + chr4.3:30405561-30405580 None:intergenic 60.0%
! CATGCCATGTGGTGGGATGC+CGG + chr4.3:30405562-30405581 None:intergenic 60.0%
!! AAACCCATGGTGGGTGCAGC+TGG + chr4.3:30403054-30403073 None:intergenic 60.0%
ATCCCCGGCATCCCACCACA+TGG - chr4.3:30405555-30405574 MS.gene031680:intron 65.0%
ATGGCCAGCTGCACCCACCA+TGG - chr4.3:30403047-30403066 MS.gene031680:intron 65.0%
GAGGAGGAGGAGGATGAGGG+GGG + chr4.3:30403077-30403096 None:intergenic 65.0%
GGAGGAGGAGGAGGATGAGG+GGG + chr4.3:30403078-30403097 None:intergenic 65.0%
GGTGTCCGGGGTTTGAACCC+TGG + chr4.3:30403974-30403993 None:intergenic 65.0%
TGGCCAGCTGCACCCACCAT+GGG - chr4.3:30403048-30403067 MS.gene031680:intron 65.0%
! GGTGCCATAGGAGGAGGAGG+AGG + chr4.3:30403087-30403106 None:intergenic 65.0%
! TGCCATGTGGTGGGATGCCG+GGG + chr4.3:30405560-30405579 None:intergenic 65.0%
! TGTGGTGGGATGCCGGGGAT+TGG + chr4.3:30405555-30405574 None:intergenic 65.0%
!! ATGGTGGGTGCAGCTGGCCA+TGG + chr4.3:30403048-30403067 None:intergenic 65.0%
!! GGTGGTGGAGGAGCTACCGA+TGG + chr4.3:30403210-30403229 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 30401434 30406085 30401434 ID=MS.gene031680
chr4.3 mRNA 30401434 30406085 30401434 ID=MS.gene031680.t1;Parent=MS.gene031680
chr4.3 exon 30405612 30406085 30405612 ID=MS.gene031680.t1.exon1;Parent=MS.gene031680.t1
chr4.3 CDS 30405612 30406085 30405612 ID=cds.MS.gene031680.t1;Parent=MS.gene031680.t1
chr4.3 exon 30404790 30404930 30404790 ID=MS.gene031680.t1.exon2;Parent=MS.gene031680.t1
chr4.3 CDS 30404790 30404930 30404790 ID=cds.MS.gene031680.t1;Parent=MS.gene031680.t1
chr4.3 exon 30404253 30404585 30404253 ID=MS.gene031680.t1.exon3;Parent=MS.gene031680.t1
chr4.3 CDS 30404253 30404585 30404253 ID=cds.MS.gene031680.t1;Parent=MS.gene031680.t1
chr4.3 exon 30402236 30402298 30402236 ID=MS.gene031680.t1.exon4;Parent=MS.gene031680.t1
chr4.3 CDS 30402236 30402298 30402236 ID=cds.MS.gene031680.t1;Parent=MS.gene031680.t1
chr4.3 exon 30401875 30401985 30401875 ID=MS.gene031680.t1.exon5;Parent=MS.gene031680.t1
chr4.3 CDS 30401875 30401985 30401875 ID=cds.MS.gene031680.t1;Parent=MS.gene031680.t1
chr4.3 exon 30401434 30401586 30401434 ID=MS.gene031680.t1.exon6;Parent=MS.gene031680.t1
chr4.3 CDS 30401434 30401586 30401434 ID=cds.MS.gene031680.t1;Parent=MS.gene031680.t1
Gene Sequence

>MS.gene031680

ATGCTTGCAGTGTCACCATTGAGGAACACTAACAAAGATGGAAACCAAGAAGAGATGGAGAGTTTTTCGATGGGGAGTACTACAATTACAACGGGCGACTTCGGAGACCTTTCCGAAGGAAATTTATTGGAAAGCATCAACTTCGACGACTTCTTTGTTGGCATCAACGTCGATGGAGACATCTTACCGGATTTGGTAATGGATCCAGAGATGTTTGCTGAGTTCTCCGTTAGTTACGGCGAGGAATCAGAGATGAATTCTTCGGCGTCACTGGAGAACAATAGCAAAGTAGAAGAGAAGAATTCAAAGAAAGATTATGAGGATAAAACAAGCTGTTCGGGTCAAGGCTCGGAAGAGATAGCGAGTAAAAGAGATGAATCTGTGGTTGTGAATCCATTGTTAAAAGATGCAGGTGGAAAAGGAAGAAAATCATCGAGTAAATCTAAGAATCCTCAAGCGAAGAGAAAAGTCAAGGTATACTTAAATAAGGAAACTATAGAGTTTTATTCTTGCTAAGTGGTTTATCTTAATTTGGTAATTAAATTCAGTGTATCTAGAGTTCATCATGTGCAGTACTAACTTTTTAGCTAAGGTTCTGTTACTGTCTTCTTGTTACGACCCTTGACAAATTATAGAGACAGAATAAAACATGAAAGATTGCAACTGTTCAAAATTTCGCTGGTAATTTTATTTTTTTTTATAGTGCGTTGTATGTTTGGTATTATTATGTGACTTTGTCATGACAAATTCATCGTGATATTCATACTAGCAATTATTAAAAAAATGTTAGCAGTGGTAGAGCTTCGAACTTTTTATAGCATTTTTTTTTCGGTTTTGTTAACAATTCAAATTTACCGGTATATTCATCGTAAATAGGATCTTCATCTCTAACGAGGCAATCCAAATATCCTCACAATTTATATATAAGTAATACTGCTATTATTATTAAATAGGAAATGTTAACGATTGAACTTAAATAGCAATTCTTTAAGAAACTTAAAGAAATAAAATTTTATGAATAATTTGCATATTACATGTCATGGAAATGAAAATATTTAATTTTTTTAAGAGTAATTTCTTGATTTGAAATCCTTAAAAAGTGCACGAGACACTCGTCAGGAAGATTCTTACGTAATTAGTGTGTTTATTGTGCAGGTGGATTGGACACCAGACTTGCACAGGAGATTTGTGCAAGCTGTGGAACAGCTAGGAGTGGACAAGGCGGTGCCTTCAAGGATTTTGGAGATTATGGAAATTGATTGTCTCACTCGCCATAACATTGCTAGTCACCTTCAGGTACATATGAATTTCAAGCATATAGGAGAAAAAGGAAATTAAATTGACATTTCGTTAATTGATTGATTGTTAAGTAAGAGTACTCGTACGATGTCTTTATGGAAAGTTATTTAAATTTTCAACTTACACTATTTATCAAGTTTTGATGGAAACTTATCACGAGTTCTAGAAGATTATATTGAGCATAATTTTTTGTTATTGCAGAAATATAGGTCACATAGAAAACATTTGCTAGCGCGTGAAGCAGAAGCAGCAAGTTGGAATCAAAGGAGACAAGTATATGGTGGTGTAGGGGTGAAGAGGGAAGTGATCAGTCCATGGCCAGCTGCACCCACCATGGGTTTCCCCCCTCATCCTCCTCCTCCTCCTATGGCACCATTGCACCATTTTAGACCCTTACATGTATGGGGACATCCTTCAAATATGGACCAGTCTTTTCTGCACATGTGGCCTACACCGCAGCCACTCTCATGGACACCATCGGTAGCTCCTCCACCACCTCAAGATCCTTCATTTTGGCATTCTCACCACCAGCAGGTAGCTCTCTCTAACCACTGAAAATAGCAGCCAAATTCCCTATATATCTATCATTGTATAGTAATACTAATAATTAAGATTTTTTTTTGGACAAAAACTAACAATTAAGATTTTAAACTTTGGAAAAAGAACACCCTTAATTATTTCAGTTTGTACTCCCGCTGGTTTCATATATAAAAACAAAGGCAAATGTTAACTTGTGCCCCAAGGGCATATGCTAAAAAATTTAAAATAGAAATAAATGTCTTAAAAGATGTGCATTTAACTTTATTCAACTTTTTAAAGTTAACATTTTGTTCTTTTTCTATGCAAAACTTCTAATTTTAGATTCTTAACTTGTGCCCTAAGGGCACAAGATAACAAGACCCAAAAACAAATTATTGTTTAGGTTCATTGAATAATTAATATATCTAATTTATAATAAAGACAAGATATATTAATTATTCGAAGAAACTAAAAAAAAAATTATATATGAGATCGGAGAGAGTATTTGATAGGAAAATATACAATTTTTAGGAGGAACACTTACTTGGGCAAAGCCCAAATAACAATATTTGAGCACACACAAAACCCAAAAAAAATATTAAAAGAGAATAAACTTAAATGATATCAATTGATGTGATTTAAGTCCTCGTGAACTTAGCTCAGTTGGTAGGGACAACGCATGGTAATATGCAAGGTCCAGGGTTCAAACCCCGCAAGGTCCAGGGTTCAAACCCCGGACACCACCAAAAAATTGATGTGACTTAATATTTTTTGTTTAATGTTGTTAATTTCATACGAGTGTGTCCAAATAATATTTATTTGATGTGACTTAATATTTTTTGTTTAATGTTGTTAATTTCATACGAGTGTGTCCAAATAATATTTATTTAAGGTTGTGTCCACGAAAGACTTTCTCTAATTTTTGATGTGACTTAATATTTTTTGTTTAATGTTGTTAATTTCATACGAGTGTGTCCAAATAATATTTATTTAAGGTTGTGTCCACGAAAGACTTTCTCTAATTTTTATTTTTTAAGGTTGTGTCCACGAAAGACTTTCTCTAATTTTTATTTTTCTCCAATTTTTATATATACCATTTGTTCGTTATATTCAAAAATTGAAGTATATATTGATATACTTGTTTTTTAAATTAATAAAGTTAACGATAATTTTTATAAAGTAAAAAATTATTTGTCGACTTTTATATTTTAGGAAATATCATTTCATAAGAAAAAAAATAACATGTCATTGTTTATAAGAGTCATATTAAATTTTCTACATTTTATCATAAAAAATCATCAATATTCAGTATTATGAGTAATAAAAATTTAGAAAAAAATAAATTTTATTTTTCCCAAATGATATTTAGTTTTTTTTTGACAAGAAATGATATTTAGTTATTATAACTGTAAATGAAAGAAAATGATTTTTTTAAAAAAAATAATACATATTCAACTATTAAATTAAAGGCCAAACGTACTTGTACATATCGAATTTTCGAGTTAACAGTAAAAATTGTCATATATTATATATTTAACTACATATTATTTGTCATATATTATATATAAGGCATGAGAGAGTTTCTTGTAGCTAGTGATAATTTTGCATCCTAGAAAAAGATTTTAAAACAATTGCTATAATAATTAAACTATTTTTGATAATTTACCAACCACATATAATTGAATCCTTGTTCCCTCAAAAAAAAAAAAAATTTGAATCCTCGTCAAAAAAGAAGAAGAACTATGTACATATAATTCAATGATGTTATTTTCAAAAAATAAAATAAAAATTCAATGATGCTGTGTGAAAATCTTGTTTGTTATAAATCAAAATTCGAGGATACATATTAATAACTATACAAATGTTTACAACAAAAATATATTAAAATTAGATCTGATTAAAATTCACGTTAATTCTTTGATTCGTGTATCTAAAATGTGATGTATTTCACTATTTCTGTGATCAACAGACTCCGATAAACGCAATGATCCGGCCAGGAACACATTGTTTCCCACCACCTCTGACAACAGTGGTAACTATTCATTACATCACTTTACTATTTTGGAGCCATGCACGTTGTCTTCTACTCTGAATTTTGGATCCTTTTTAAAGATAAGATTGACAGTATACAAATTTTAGTATTGTCATATTTACCAACATATATAAATATAGCACATAATTATATATGTTGTCGTATATATATTTAGTCACAAACACATAACACAAATTATTCTCCAAATATAAGTTTGGCTGACCATAAAGGAAAATAACATAACATGCAGAGACCATCATTTGGATGTCCAATCCCCGGCATCCCACCACATGGCATGTGTAAAGCAGATCACGGCATTAGCACCCTTCCTGCTCCCGGTCACTTCTTCGACTTCCCACCGGTACTGAATTTCCTTCGCCATACATACATACATGCATTCAACATCTTTTTTTCTTCAATTCTCTTTATTTAGTTTCCGGCCATATATTAAAAAGTTTCACATTGTATGAGAGACGATAAGGGCATGTGTTTATAAGGTGGGAGTCATTGCTCACTTTATAAGACGATTTTTTAACGATGAGTTAACCCCAATATAAAAACACCTAAAAGCTCCATAGATTTCAAGGCATGAGTAATTAGATGATTCGTTGTTTGTATGCTAATGTATTTTTATAAATTTATTTTACAGTCAAATGAGAGCATAGACGCAGCTATTGGAGATGTTTTATCAAAACCATGGTTGCCACTACCACTTGGACTCAAAGCTCCAGCACTTGATAGCGTAATGGGTGAACTACAAAGACAAGGAATTTCAAACATTCCACCCTCATCATGCACTTGA
Protein sequence

>MS.gene031680.t1

MLAVSPLRNTNKDGNQEEMESFSMGSTTITTGDFGDLSEGNLLESINFDDFFVGINVDGDILPDLVMDPEMFAEFSVSYGEESEMNSSASLENNSKVEEKNSKKDYEDKTSCSGQGSEEIASKRDESVVVNPLLKDAGGKGRKSSSKSKNPQAKRKVKVDWTPDLHRRFVQAVEQLGVDKAVPSRILEIMEIDCLTRHNIASHLQKYRSHRKHLLAREAEAASWNQRRQVYGGVGVKREVISPWPAAPTMGFPPHPPPPPMAPLHHFRPLHVWGHPSNMDQSFLHMWPTPQPLSWTPSVAPPPPQDPSFWHSHHQQTPINAMIRPGTHCFPPPLTTVRPSFGCPIPGIPPHGMCKADHGISTLPAPGHFFDFPPSNESIDAAIGDVLSKPWLPLPLGLKAPALDSVMGELQRQGISNIPPSSCT