Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031709.t1 | XP_003607469.1 | 95.2 | 208 | 9 | 1 | 1 | 207 | 1 | 208 | 3.50E-105 | 391 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031709.t1 | F4JIJ7 | 42.9 | 189 | 88 | 8 | 8 | 180 | 2 | 186 | 3.4e-24 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031709.t1 | G7JTT7 | 95.2 | 208 | 9 | 1 | 1 | 207 | 1 | 208 | 2.5e-105 | 391.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene031709.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031709.t1 | MTR_4g078350 | 95.192 | 208 | 9 | 1 | 1 | 207 | 1 | 208 | 1.26e-145 | 404 |
| MS.gene031709.t1 | MTR_2g090480 | 64.000 | 200 | 61 | 4 | 8 | 201 | 9 | 203 | 2.44e-82 | 244 |
| MS.gene031709.t1 | MTR_2g090440 | 64.467 | 197 | 63 | 4 | 8 | 201 | 9 | 201 | 7.13e-82 | 243 |
| MS.gene031709.t1 | MTR_2g090425 | 65.152 | 198 | 59 | 5 | 8 | 199 | 9 | 202 | 3.36e-79 | 240 |
| MS.gene031709.t1 | MTR_2g090500 | 59.236 | 157 | 59 | 3 | 48 | 201 | 60 | 214 | 1.36e-56 | 179 |
| MS.gene031709.t1 | MTR_2g013920 | 46.667 | 180 | 79 | 8 | 7 | 180 | 3 | 171 | 1.28e-40 | 136 |
| MS.gene031709.t1 | MTR_2g090515 | 70.000 | 50 | 12 | 1 | 8 | 57 | 9 | 55 | 1.83e-14 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031709.t1 | AT4G20970 | 42.328 | 189 | 89 | 8 | 8 | 180 | 2 | 186 | 7.18e-32 | 114 |
| MS.gene031709.t1 | AT1G10585 | 28.161 | 174 | 114 | 6 | 11 | 182 | 11 | 175 | 2.31e-12 | 63.2 |
| MS.gene031709.t1 | AT1G10586 | 27.326 | 172 | 114 | 6 | 11 | 180 | 11 | 173 | 1.96e-11 | 60.5 |
Find 43 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCCATCTTGTGTTCAATT+TGG | 0.181511 | 4.3:-30721776 | MS.gene031709:CDS |
| ATTTCAAAACATGGTGATTT+TGG | 0.208325 | 4.3:+30722065 | None:intergenic |
| AGAACCTTGGGGTTATAGTT+AGG | 0.280194 | 4.3:+30722481 | None:intergenic |
| TATCTAATTATGGATCAAAT+TGG | 0.283616 | 4.3:-30722613 | None:intergenic |
| AGGGAGAAGAGAGTTGAGTT+TGG | 0.296752 | 4.3:+30722501 | None:intergenic |
| GAGAGTTGAGTTTGGAGTAG+AGG | 0.397503 | 4.3:+30722509 | None:intergenic |
| TGAATTATCTCCAACCTTAG+AGG | 0.408147 | 4.3:+30721923 | None:intergenic |
| GGAATTACCGTTGGCGCTAC+CGG | 0.409114 | 4.3:-30722213 | MS.gene031709:CDS |
| AACCAGCCTTCTTTCAACTT+TGG | 0.410390 | 4.3:+30722561 | None:intergenic |
| GAACCTTGGGGTTATAGTTA+GGG | 0.410715 | 4.3:+30722482 | None:intergenic |
| TGATTTCATAGAAAATGAAT+TGG | 0.413742 | 4.3:+30721991 | None:intergenic |
| AAGGAGTGTCAATTCCTCTA+AGG | 0.421100 | 4.3:-30721937 | MS.gene031709:CDS |
| AAGAAAGAAAGGTTATTAGC+TGG | 0.449807 | 4.3:-30722125 | MS.gene031709:CDS |
| ATCGCCTCATCTACTTGATC+CGG | 0.457881 | 4.3:+30722194 | None:intergenic |
| AGATTGAGAAGGTTTGTGTA+TGG | 0.481420 | 4.3:-30721734 | MS.gene031709:CDS |
| TTGGTTGTATTAGAGCTAGA+AGG | 0.500610 | 4.3:+30722580 | None:intergenic |
| ATGAAGAGAATGTAGACATA+AGG | 0.503791 | 4.3:-30721956 | MS.gene031709:CDS |
| AGATCACGTGAATATTGCTT+AGG | 0.512635 | 4.3:-30722092 | MS.gene031709:CDS |
| TTGGAACTGATCATAATATG+TGG | 0.512688 | 4.3:-30721664 | MS.gene031709:CDS |
| AGGCTGTATTTCCACATCAC+TGG | 0.522661 | 4.3:+30721706 | None:intergenic |
| ATACAACCAAAGTTGAAAGA+AGG | 0.526780 | 4.3:-30722567 | MS.gene031709:CDS |
| TATAATTCAGGAATTACCGT+TGG | 0.530375 | 4.3:-30722222 | MS.gene031709:intron |
| ACTTGATCCGGTAGCGCCAA+CGG | 0.534008 | 4.3:+30722206 | None:intergenic |
| ATTTCTTGAATTTCAAAACA+TGG | 0.541430 | 4.3:+30722056 | None:intergenic |
| CCTTCATGTTGTTCATGCTG+AGG | 0.541669 | 4.3:-30721898 | MS.gene031709:intron |
| AACCAAAGTTGAAAGAAGGC+TGG | 0.543583 | 4.3:-30722563 | MS.gene031709:CDS |
| TGGAACTGATCATAATATGT+GGG | 0.553773 | 4.3:-30721663 | MS.gene031709:CDS |
| CTACAAATCATTCTCACGTG+TGG | 0.558837 | 4.3:-30722023 | MS.gene031709:CDS |
| AGTGTCAATTCCTCTAAGGT+TGG | 0.561526 | 4.3:-30721933 | MS.gene031709:CDS |
| ATTTCAAAATCCCACAACTC+AGG | 0.570407 | 4.3:+30721686 | None:intergenic |
| AAGTGAGTGAGAGATTGAGA+AGG | 0.570785 | 4.3:-30721745 | MS.gene031709:CDS |
| TGGCTAATGAGAAGAAAGAA+AGG | 0.571122 | 4.3:-30722136 | MS.gene031709:CDS |
| TACAAATCATTCTCACGTGT+GGG | 0.575180 | 4.3:-30722022 | MS.gene031709:CDS |
| GCTCCAAATTGAACACAAGA+TGG | 0.580457 | 4.3:+30721773 | None:intergenic |
| GGAAATACAGCCTGAGTTGT+GGG | 0.582012 | 4.3:-30721696 | MS.gene031709:CDS |
| TTTAGAGGCAAATGTGCAGA+TGG | 0.585387 | 4.3:-30722156 | MS.gene031709:CDS |
| TGGAAATACAGCCTGAGTTG+TGG | 0.593486 | 4.3:-30721697 | MS.gene031709:CDS |
| CCTCAGCATGAACAACATGA+AGG | 0.636646 | 4.3:+30721898 | None:intergenic |
| GGAACTGATCATAATATGTG+GGG | 0.640483 | 4.3:-30721662 | MS.gene031709:CDS |
| GTATGGATCTTCCAGTGATG+TGG | 0.643608 | 4.3:-30721717 | MS.gene031709:CDS |
| TCTCCCTAACTATAACCCCA+AGG | 0.657270 | 4.3:-30722485 | MS.gene031709:intron |
| CCAAATTGAACACAAGATGG+AGG | 0.671279 | 4.3:+30721776 | None:intergenic |
| GCTACCGGATCAAGTAGATG+AGG | 0.685176 | 4.3:-30722198 | MS.gene031709:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAAAAAAAAAAAAAGT+TGG | - | chr4.3:30721837-30721856 | MS.gene031709:intron | 10.0% |
| !! | AAACTACATAAAAAGTTTAG+AGG | - | chr4.3:30722088-30722107 | MS.gene031709:CDS | 20.0% |
| !! | ATTTCTTGAATTTCAAAACA+TGG | + | chr4.3:30722206-30722225 | None:intergenic | 20.0% |
| !! | TGATTTCATAGAAAATGAAT+TGG | + | chr4.3:30722271-30722290 | None:intergenic | 20.0% |
| !!! | TGATGTCATTTTTATAATTC+AGG | - | chr4.3:30722025-30722044 | MS.gene031709:CDS | 20.0% |
| !!! | TGTTTTGAAATTCAAGAAAT+TGG | - | chr4.3:30722206-30722225 | MS.gene031709:CDS | 20.0% |
| ! | AAAGAAAGAAATTAGAACCT+TGG | + | chr4.3:30721794-30721813 | None:intergenic | 25.0% |
| ! | AAGAAAACAACTAACTAACA+TGG | + | chr4.3:30722001-30722020 | None:intergenic | 25.0% |
| ! | AAGAAAGAAATTAGAACCTT+GGG | + | chr4.3:30721793-30721812 | None:intergenic | 25.0% |
| ! | ATAAAGAGAGAACAATTACT+AGG | + | chr4.3:30722456-30722475 | None:intergenic | 25.0% |
| !! | AAAAAAAAAGTTGGTTACAC+TGG | - | chr4.3:30721846-30721865 | MS.gene031709:intron | 25.0% |
| !! | ATTTCAAAACATGGTGATTT+TGG | + | chr4.3:30722197-30722216 | None:intergenic | 25.0% |
| !!! | ACTAACATGGTTTTAATTTG+TGG | + | chr4.3:30721988-30722007 | None:intergenic | 25.0% |
| AAGAAAGAAAGGTTATTAGC+TGG | - | chr4.3:30722134-30722153 | MS.gene031709:CDS | 30.0% | |
| AGAAAGAAATTAGAACCTTG+GGG | + | chr4.3:30721792-30721811 | None:intergenic | 30.0% | |
| ATACAACCAAAGTTGAAAGA+AGG | - | chr4.3:30721692-30721711 | MS.gene031709:CDS | 30.0% | |
| ATGAAGAGAATGTAGACATA+AGG | - | chr4.3:30722303-30722322 | MS.gene031709:intron | 30.0% | |
| TATAATTCAGGAATTACCGT+TGG | - | chr4.3:30722037-30722056 | MS.gene031709:CDS | 30.0% | |
| TGGAACTGATCATAATATGT+GGG | - | chr4.3:30722596-30722615 | MS.gene031709:CDS | 30.0% | |
| TTGGAACTGATCATAATATG+TGG | - | chr4.3:30722595-30722614 | MS.gene031709:CDS | 30.0% | |
| AGATCACGTGAATATTGCTT+AGG | - | chr4.3:30722167-30722186 | MS.gene031709:CDS | 35.0% | |
| AGATTGAGAAGGTTTGTGTA+TGG | - | chr4.3:30722525-30722544 | MS.gene031709:CDS | 35.0% | |
| ATTTCAAAATCCCACAACTC+AGG | + | chr4.3:30722576-30722595 | None:intergenic | 35.0% | |
| GGAACTGATCATAATATGTG+GGG | - | chr4.3:30722597-30722616 | MS.gene031709:CDS | 35.0% | |
| TACAAATCATTCTCACGTGT+GGG | - | chr4.3:30722237-30722256 | MS.gene031709:intron | 35.0% | |
| TGAATTATCTCCAACCTTAG+AGG | + | chr4.3:30722339-30722358 | None:intergenic | 35.0% | |
| TGGCTAATGAGAAGAAAGAA+AGG | - | chr4.3:30722123-30722142 | MS.gene031709:CDS | 35.0% | |
| TTGGTTGTATTAGAGCTAGA+AGG | + | chr4.3:30721682-30721701 | None:intergenic | 35.0% | |
| !!! | GAGTTGTGGGATTTTGAAAT+TGG | - | chr4.3:30722576-30722595 | MS.gene031709:CDS | 35.0% |
| AACCAAAGTTGAAAGAAGGC+TGG | - | chr4.3:30721696-30721715 | MS.gene031709:CDS | 40.0% | |
| AACCAGCCTTCTTTCAACTT+TGG | + | chr4.3:30721701-30721720 | None:intergenic | 40.0% | |
| AAGCCACAATATTGTGGTTG+TGG | - | chr4.3:30721944-30721963 | MS.gene031709:CDS | 40.0% | |
| AAGTGAGTGAGAGATTGAGA+AGG | - | chr4.3:30722514-30722533 | MS.gene031709:CDS | 40.0% | |
| AGAACCTTGGGGTTATAGTT+AGG | + | chr4.3:30721781-30721800 | None:intergenic | 40.0% | |
| ATGACGAAGCCACAATATTG+TGG | - | chr4.3:30721938-30721957 | MS.gene031709:CDS | 40.0% | |
| CCAAATTGAACACAAGATGG+AGG | + | chr4.3:30722486-30722505 | None:intergenic | 40.0% | |
| CCTCCATCTTGTGTTCAATT+TGG | - | chr4.3:30722483-30722502 | MS.gene031709:intron | 40.0% | |
| CTACAAATCATTCTCACGTG+TGG | - | chr4.3:30722236-30722255 | MS.gene031709:intron | 40.0% | |
| CTACCACAACCACAATATTG+TGG | + | chr4.3:30721950-30721969 | None:intergenic | 40.0% | |
| GAACCTTGGGGTTATAGTTA+GGG | + | chr4.3:30721780-30721799 | None:intergenic | 40.0% | |
| GCTCCAAATTGAACACAAGA+TGG | + | chr4.3:30722489-30722508 | None:intergenic | 40.0% | |
| TTTAGAGGCAAATGTGCAGA+TGG | - | chr4.3:30722103-30722122 | MS.gene031709:CDS | 40.0% | |
| !! | AAGGAGTGTCAATTCCTCTA+AGG | - | chr4.3:30722322-30722341 | MS.gene031709:intron | 40.0% |
| !! | AGTGTCAATTCCTCTAAGGT+TGG | - | chr4.3:30722326-30722345 | MS.gene031709:intron | 40.0% |
| AGGCTGTATTTCCACATCAC+TGG | + | chr4.3:30722556-30722575 | None:intergenic | 45.0% | |
| AGGGAGAAGAGAGTTGAGTT+TGG | + | chr4.3:30721761-30721780 | None:intergenic | 45.0% | |
| ATCGCCTCATCTACTTGATC+CGG | + | chr4.3:30722068-30722087 | None:intergenic | 45.0% | |
| CCTCAGCATGAACAACATGA+AGG | + | chr4.3:30722364-30722383 | None:intergenic | 45.0% | |
| CCTTCATGTTGTTCATGCTG+AGG | - | chr4.3:30722361-30722380 | MS.gene031709:intron | 45.0% | |
| GGAAATACAGCCTGAGTTGT+GGG | - | chr4.3:30722563-30722582 | MS.gene031709:CDS | 45.0% | |
| GTATGGATCTTCCAGTGATG+TGG | - | chr4.3:30722542-30722561 | MS.gene031709:CDS | 45.0% | |
| TCTCCCTAACTATAACCCCA+AGG | - | chr4.3:30721774-30721793 | MS.gene031709:CDS | 45.0% | |
| TGGAAATACAGCCTGAGTTG+TGG | - | chr4.3:30722562-30722581 | MS.gene031709:CDS | 45.0% | |
| ! | GAGAGTTGAGTTTGGAGTAG+AGG | + | chr4.3:30721753-30721772 | None:intergenic | 45.0% |
| GCTACCGGATCAAGTAGATG+AGG | - | chr4.3:30722061-30722080 | MS.gene031709:CDS | 50.0% | |
| ACTTGATCCGGTAGCGCCAA+CGG | + | chr4.3:30722056-30722075 | None:intergenic | 55.0% | |
| GGAATTACCGTTGGCGCTAC+CGG | - | chr4.3:30722046-30722065 | MS.gene031709:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 30721655 | 30722626 | 30721655 | ID=MS.gene031709 |
| chr4.3 | mRNA | 30721655 | 30722626 | 30721655 | ID=MS.gene031709.t1;Parent=MS.gene031709 |
| chr4.3 | exon | 30722486 | 30722626 | 30722486 | ID=MS.gene031709.t1.exon1;Parent=MS.gene031709.t1 |
| chr4.3 | CDS | 30722486 | 30722626 | 30722486 | ID=cds.MS.gene031709.t1;Parent=MS.gene031709.t1 |
| chr4.3 | exon | 30721899 | 30722234 | 30721899 | ID=MS.gene031709.t1.exon2;Parent=MS.gene031709.t1 |
| chr4.3 | CDS | 30721899 | 30722234 | 30721899 | ID=cds.MS.gene031709.t1;Parent=MS.gene031709.t1 |
| chr4.3 | exon | 30721655 | 30721801 | 30721655 | ID=MS.gene031709.t1.exon3;Parent=MS.gene031709.t1 |
| chr4.3 | CDS | 30721655 | 30721801 | 30721655 | ID=cds.MS.gene031709.t1;Parent=MS.gene031709.t1 |
| Gene Sequence |
| Protein sequence |
