AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031756

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031756.t1 MTR_4g077760 96.780 559 14 2 1 559 1 555 0.0 1102
MS.gene031756.t1 MTR_2g082090 37.892 351 193 11 223 558 238 578 2.79e-53 191
MS.gene031756.t1 MTR_3g056110 35.593 354 211 10 217 559 195 542 6.34e-53 189
MS.gene031756.t1 MTR_5g094450 34.026 385 233 9 190 559 154 532 1.36e-52 187
MS.gene031756.t1 MTR_3g065980 34.884 387 216 12 194 559 169 540 5.84e-52 186
MS.gene031756.t1 MTR_7g074650 34.060 367 220 10 200 559 441 792 1.07e-51 189
MS.gene031756.t1 MTR_7g027190 31.529 425 235 11 160 559 280 673 1.43e-51 187
MS.gene031756.t1 MTR_3g089055 31.383 376 235 8 198 559 199 565 5.24e-50 181
MS.gene031756.t1 MTR_3g089055 31.383 376 235 8 198 559 204 570 5.57e-50 181
MS.gene031756.t1 MTR_5g097480 33.684 380 237 9 190 559 170 544 3.07e-49 179
MS.gene031756.t1 MTR_1g069725 33.871 372 224 10 194 558 106 462 3.71e-49 177
MS.gene031756.t1 MTR_1g029420 31.183 372 241 7 198 559 226 592 4.13e-49 179
MS.gene031756.t1 MTR_4g076020 31.944 360 203 12 211 559 101 429 7.71e-44 162
MS.gene031756.t1 MTR_4g102790 30.287 383 239 12 194 558 289 661 8.93e-44 166
MS.gene031756.t1 MTR_4g104020 27.792 385 249 7 194 559 146 520 3.95e-43 161
MS.gene031756.t1 MTR_4g133660 32.286 350 220 9 220 558 199 542 4.05e-43 162
MS.gene031756.t1 MTR_1g096030 31.392 395 232 11 193 559 76 459 2.55e-42 158
MS.gene031756.t1 MTR_2g026250 30.571 350 228 8 220 559 252 596 5.26e-40 154
MS.gene031756.t1 MTR_4g097080 28.974 390 251 10 185 559 125 503 7.98e-40 152
MS.gene031756.t1 MTR_5g058860 31.232 349 223 6 225 558 150 496 1.74e-38 148
MS.gene031756.t1 MTR_3g072710 29.216 421 249 9 184 558 82 499 2.07e-38 148
MS.gene031756.t1 MTR_3g022830 28.009 432 239 13 188 559 17 436 5.26e-38 145
MS.gene031756.t1 MTR_4g064200 27.937 383 254 9 189 558 276 649 2.06e-37 147
MS.gene031756.t1 MTR_2g097310 28.684 380 244 10 195 558 269 637 2.18e-37 147
MS.gene031756.t1 MTR_2g097410 26.772 381 254 10 195 558 368 740 1.62e-36 145
MS.gene031756.t1 MTR_4g064160 29.024 379 247 9 194 558 313 683 2.20e-36 144
MS.gene031756.t1 MTR_2g097350 28.421 380 245 10 195 558 271 639 8.17e-36 142
MS.gene031756.t1 MTR_4g064150 27.473 364 238 9 213 558 377 732 9.16e-36 142
MS.gene031756.t1 MTR_8g442410 28.571 385 245 10 194 559 152 525 9.88e-36 140
MS.gene031756.t1 MTR_2g097390 28.684 380 244 10 195 558 272 640 1.55e-35 141
MS.gene031756.t1 MTR_5g015490 29.771 393 240 13 190 559 111 490 1.98e-35 139
MS.gene031756.t1 MTR_5g009080 25.688 436 244 9 194 559 53 478 2.16e-35 139
MS.gene031756.t1 MTR_2g097467 25.729 377 259 7 194 558 286 653 6.52e-35 139
MS.gene031756.t1 MTR_2g097463 25.381 394 271 8 178 558 271 654 1.25e-34 139
MS.gene031756.t1 MTR_8g093070 32.394 355 212 14 214 558 159 495 2.08e-34 136
MS.gene031756.t1 MTR_4g064120 25.693 397 277 8 166 548 223 615 2.99e-34 137
MS.gene031756.t1 MTR_4g076140 26.545 437 238 12 194 559 45 469 4.33e-34 135
MS.gene031756.t1 MTR_2g034250 26.804 388 253 11 194 559 207 585 4.93e-34 136
MS.gene031756.t1 MTR_4g064180 27.714 350 235 8 213 548 270 615 1.32e-33 135
MS.gene031756.t1 MTR_2g034260 26.302 384 259 10 194 559 207 584 1.52e-33 135
MS.gene031756.t1 MTR_2g034280 26.316 380 262 8 194 559 200 575 2.20e-33 134
MS.gene031756.t1 MTR_2g097473 25.729 377 259 7 194 558 286 653 2.52e-33 135
MS.gene031756.t1 MTR_4g026485 29.639 415 240 16 167 559 240 624 6.23e-33 133
MS.gene031756.t1 MTR_7g062120 26.836 354 241 8 220 559 391 740 1.37e-32 133
MS.gene031756.t1 MTR_7g109580 28.804 368 245 10 202 559 194 554 7.75e-32 129
MS.gene031756.t1 MTR_0092s0100 30.667 375 236 12 194 559 369 728 6.78e-31 128
MS.gene031756.t1 MTR_0092s0100 30.667 375 236 12 194 559 372 731 7.69e-31 128
MS.gene031756.t1 MTR_2g097380 26.703 367 218 10 205 558 232 560 1.01e-30 126
MS.gene031756.t1 MTR_2g099110 27.735 393 241 11 196 559 73 451 1.24e-28 119
MS.gene031756.t1 MTR_2g089100 26.316 399 251 13 196 558 51 442 6.01e-26 111
MS.gene031756.t1 MTR_7g057230 29.017 417 256 16 162 558 260 656 3.90e-23 103
MS.gene031756.t1 MTR_5g015950 24.429 438 263 13 184 558 13 445 4.81e-23 102
MS.gene031756.t1 MTR_3g053270 24.429 438 263 13 184 558 13 445 4.81e-23 102
MS.gene031756.t1 MTR_4g095500 23.673 452 276 14 161 558 31 467 8.78e-20 92.8
MS.gene031756.t1 MTR_1g086970 21.978 364 246 12 219 559 130 478 5.36e-19 90.5
MS.gene031756.t1 MTR_8g020840 25.260 384 258 10 198 559 172 548 8.57e-19 90.1
MS.gene031756.t1 MTR_8g077940 24.936 389 243 14 194 559 179 541 8.27e-16 80.9
MS.gene031756.t1 MTR_7g069740 23.823 361 235 13 222 559 241 584 5.03e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031756.t1 AT5G66770 54.010 611 202 13 1 559 1 584 0.0 535
MS.gene031756.t1 AT3G50650 49.744 587 222 14 1 559 1 542 3.01e-161 471
MS.gene031756.t1 AT5G41920 32.843 408 233 12 170 559 16 400 9.26e-55 190
MS.gene031756.t1 AT5G48150 33.596 381 237 7 190 559 115 490 5.24e-53 188
MS.gene031756.t1 AT5G48150 33.596 381 237 7 190 559 115 490 5.24e-53 188
MS.gene031756.t1 AT5G48150 33.596 381 237 7 190 559 115 490 5.24e-53 188
MS.gene031756.t1 AT5G48150 33.596 381 237 7 190 559 115 490 5.24e-53 188
MS.gene031756.t1 AT1G50600 34.211 380 235 8 190 559 152 526 5.69e-53 189
MS.gene031756.t1 AT1G50600 34.211 380 235 8 190 559 223 597 2.67e-52 188
MS.gene031756.t1 AT3G03450 31.410 468 279 18 119 559 93 545 3.01e-52 187
MS.gene031756.t1 AT1G50600 34.211 380 235 8 190 559 227 601 3.48e-52 188
MS.gene031756.t1 AT3G54220 33.784 370 218 11 200 558 296 649 7.86e-52 188
MS.gene031756.t1 AT2G04890 34.085 355 210 12 216 559 72 413 5.56e-49 175
MS.gene031756.t1 AT5G17490 32.061 393 244 12 177 559 137 516 1.73e-45 168
MS.gene031756.t1 AT1G66350 29.149 470 276 16 112 559 72 506 6.18e-45 166
MS.gene031756.t1 AT1G21450 28.250 400 272 8 170 559 199 593 8.16e-45 167
MS.gene031756.t1 AT1G63100 31.776 321 184 8 260 558 321 628 7.23e-43 162
MS.gene031756.t1 AT1G63100 31.776 321 184 8 260 558 346 653 7.71e-43 162
MS.gene031756.t1 AT4G17230 31.649 376 242 8 194 559 155 525 5.38e-42 158
MS.gene031756.t1 AT4G17230 31.649 376 242 8 194 559 155 525 5.38e-42 158
MS.gene031756.t1 AT2G01570 33.141 347 206 14 223 559 251 581 1.46e-41 158
MS.gene031756.t1 AT1G55580 31.579 380 205 9 227 559 74 445 4.43e-41 154
MS.gene031756.t1 AT1G14920 31.232 349 210 12 223 559 199 529 3.22e-40 154
MS.gene031756.t1 AT3G46600 27.763 371 255 7 198 559 5 371 9.26e-40 149
MS.gene031756.t1 AT3G46600 27.763 371 255 7 198 559 213 579 6.28e-39 150
MS.gene031756.t1 AT3G46600 27.763 371 255 7 198 559 181 547 9.66e-39 149
MS.gene031756.t1 AT4G08250 29.429 350 237 5 217 558 129 476 1.33e-38 148
MS.gene031756.t1 AT5G59450 26.551 403 278 8 171 559 200 598 6.29e-38 148
MS.gene031756.t1 AT1G07520 28.382 377 251 7 198 558 319 692 8.26e-38 148
MS.gene031756.t1 AT1G07520 28.382 377 251 7 198 558 320 693 9.66e-38 148
MS.gene031756.t1 AT4G37650 29.101 378 238 11 206 559 158 529 9.81e-38 146
MS.gene031756.t1 AT1G07520 28.382 377 251 7 198 558 155 528 1.03e-37 146
MS.gene031756.t1 AT1G07520 28.382 377 251 7 198 558 155 528 1.03e-37 146
MS.gene031756.t1 AT1G07530 26.633 398 270 9 175 558 375 764 2.90e-36 144
MS.gene031756.t1 AT2G29065 27.763 389 239 10 198 558 253 627 7.87e-36 142
MS.gene031756.t1 AT2G29060 24.524 420 300 9 152 558 276 691 2.53e-35 141
MS.gene031756.t1 AT2G29060 24.524 420 300 9 152 558 239 654 2.93e-35 140
MS.gene031756.t1 AT2G37650 25.217 345 236 7 228 559 378 713 6.16e-28 119
MS.gene031756.t1 AT2G37650 25.217 345 236 7 228 559 366 701 6.62e-28 119
MS.gene031756.t1 AT5G52510 26.615 387 248 12 196 559 267 640 2.03e-26 114
MS.gene031756.t1 AT4G00150 28.302 371 219 14 209 559 211 554 7.57e-26 112
MS.gene031756.t1 AT2G45160 28.070 399 227 16 194 559 268 639 1.51e-23 105
MS.gene031756.t1 AT3G60630 29.412 306 190 9 268 559 329 622 1.87e-23 105
MS.gene031756.t1 AT1G50420 31.250 224 137 5 211 428 74 286 2.75e-23 103
MS.gene031756.t1 AT1G50420 32.414 145 90 3 420 559 338 479 6.85e-15 77.8
MS.gene031756.t1 AT3G49950 27.171 357 226 12 227 558 60 407 1.23e-22 100
MS.gene031756.t1 AT3G13840 25.722 381 249 9 196 559 147 510 1.03e-20 95.9
MS.gene031756.t1 AT4G36710 23.947 380 259 12 194 559 122 485 4.81e-17 84.3

Find 125 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATAGTGTAAGAATTTC+TGG 0.119456 4.3:+31156290 MS.gene031756:CDS
GATATTCATATAGTTGATTT+TGG 0.147557 4.3:+31156203 MS.gene031756:CDS
CTCTATTGACTGTTTCTTCT+TGG 0.170351 4.3:+31156960 MS.gene031756:CDS
CCAGAAATTCTTACACTATT+AGG 0.205458 4.3:-31156290 None:intergenic
ATATGGGCATGCATCGTTTA+AGG 0.214693 4.3:-31156120 None:intergenic
GCTTCAGTTGTATAATCTTT+TGG 0.233520 4.3:+31156499 MS.gene031756:CDS
TAAAAGGAAACTCTTTGATT+AGG 0.234171 4.3:-31155996 None:intergenic
GGTATACCTGCTATGGCTTT+AGG 0.252034 4.3:+31156311 MS.gene031756:CDS
AAATGTGCGAACTTTGAATA+TGG 0.257358 4.3:-31156137 None:intergenic
TGGGTGGTTGGATTGGGATT+TGG 0.269914 4.3:-31155797 None:intergenic
AAAAGGAAACTCTTTGATTA+GGG 0.285344 4.3:-31155995 None:intergenic
AATCCTAAGATTGTTACTTT+AGG 0.287104 4.3:+31156572 MS.gene031756:CDS
TCACCTAAAGTAACAATCTT+AGG 0.289550 4.3:-31156575 None:intergenic
TGCGGAGTGGGTGGTTGGAT+TGG 0.299788 4.3:-31155804 None:intergenic
AGGGTTTCAATTGCTTGATT+TGG 0.313945 4.3:-31155936 None:intergenic
TAAGGCTTGTGAAAAGTAAA+AGG 0.314801 4.3:-31156012 None:intergenic
TTCGATGAGTGAAGCAATTT+CGG 0.323142 4.3:-31155904 None:intergenic
CAAATTCTTCTTCAACTACT+TGG 0.323189 4.3:+31156072 MS.gene031756:CDS
CAATTGCTTGATTTGGTTTG+TGG 0.338888 4.3:-31155929 None:intergenic
CTTGTTGAGCTATTGCCATT+AGG 0.345192 4.3:-31155338 None:intergenic
GCGGAGTGGGTGGTTGGATT+GGG 0.350584 4.3:-31155803 None:intergenic
TTGATTTGCTGAGATGGGTT+AGG 0.352847 4.3:-31155956 None:intergenic
TTGTGATACCTAGGATGTTT+TGG 0.353661 4.3:-31155431 None:intergenic
GCATTTGCAACTCGTTCTTC+TGG 0.357528 4.3:+31156263 MS.gene031756:CDS
TTAACAGCTAAAGCCTCATT+TGG 0.363944 4.3:-31156470 None:intergenic
TTGAAATGGGTCGGTGAAAT+CGG 0.367983 4.3:-31155550 None:intergenic
GGACCCGACCCGACTTGAAA+TGG 0.372343 4.3:-31155564 None:intergenic
AATTGCTTGATTTGGTTTGT+GGG 0.376792 4.3:-31155928 None:intergenic
CCAGTCATCGGAGTCGAAAT+CGG 0.397137 4.3:-31155655 None:intergenic
CCGATTTCGACTCCGATGAC+TGG 0.399290 4.3:+31155655 MS.gene031756:CDS
TGATTTGCTGAGATGGGTTA+GGG 0.400674 4.3:-31155955 None:intergenic
ATTTGGAGTGGCACGGTGGC+GGG 0.405277 4.3:-31155780 None:intergenic
TGAAAACGACGATGTCGTTT+CGG 0.406416 4.3:-31155850 None:intergenic
TTGCTTGGTAGGAGAATTGA+TGG 0.408569 4.3:+31156737 MS.gene031756:CDS
GACCCGACTCTCACCGCAAC+CGG 0.408658 4.3:+31155746 MS.gene031756:CDS
TGAGTGAAGCAATTTCGGTT+AGG 0.410733 4.3:-31155899 None:intergenic
GAGTGAAGCAATTTCGGTTA+GGG 0.413751 4.3:-31155898 None:intergenic
GAATTGTTCAAGGGATTCAA+TGG 0.414040 4.3:+31156225 MS.gene031756:CDS
ATCCGGTTGCGGTGAGAGTC+GGG 0.422803 4.3:-31155748 None:intergenic
GATTTGGAGTGGCACGGTGG+CGG 0.424354 4.3:-31155781 None:intergenic
AGTGATGAAGAAGAAGATAC+TGG 0.431284 4.3:-31155504 None:intergenic
GATTCATCAAGAAGCTCAAT+AGG 0.436690 4.3:-31156431 None:intergenic
GATGATGATGTTGATGTTGT+TGG 0.436789 4.3:-31155407 None:intergenic
GTGAAATTGATTTGCTGAGA+TGG 0.442057 4.3:-31155962 None:intergenic
GGCGAATCCAACGCCATGTT+CGG 0.446437 4.3:-31156689 None:intergenic
AAACTCAGATAAACGATTAC+CGG 0.454956 4.3:-31156363 None:intergenic
TCACCGACCCATTTCAAGTC+GGG 0.456993 4.3:+31155556 MS.gene031756:CDS
ATCGGAGTGGTTGTCGGCAG+CGG 0.470784 4.3:-31155694 None:intergenic
GTGTGATTGGTGTGAGGGAG+AGG 0.470960 4.3:+31156759 MS.gene031756:CDS
GTAGCTGAAATCGATGAAAC+TGG 0.471542 4.3:-31156341 None:intergenic
AATTTCTGGTATACCTGCTA+TGG 0.471549 4.3:+31156304 MS.gene031756:CDS
GATTGGTGTGAGGGAGAGGA+TGG 0.472918 4.3:+31156763 MS.gene031756:CDS
AATGTGCGAACTTTGAATAT+GGG 0.473696 4.3:-31156136 None:intergenic
GACCCATTTCAAGTCGGGTC+GGG 0.475045 4.3:+31155561 MS.gene031756:CDS
AGCAAATCAATTTCACAAAA+CGG 0.480108 4.3:+31155969 MS.gene031756:CDS
CGCTGACAGTGGAAACCTAA+TGG 0.480605 4.3:+31155323 MS.gene031756:CDS
ATCAACTATATGAATATCGT+TGG 0.482770 4.3:-31156198 None:intergenic
CTCTCCACTTGAAACCTCTC+TGG 0.491073 4.3:-31156710 None:intergenic
ATGGAAATCGGAGTGGTTGT+CGG 0.496680 4.3:-31155700 None:intergenic
CGGGTCGGGCATGATGAAAA+CGG 0.497777 4.3:-31155729 None:intergenic
GTTGTCGGCAGCGGACATTA+AGG 0.498733 4.3:-31155685 None:intergenic
CGAAGCTAGTTTGACGACTC+GGG 0.501254 4.3:+31156601 MS.gene031756:CDS
TGAAATTGATTTGCTGAGAT+GGG 0.503445 4.3:-31155961 None:intergenic
TGCGTTGCGGAGTGGGTGGT+TGG 0.505785 4.3:-31155809 None:intergenic
AGGAGAATTGATGGTGTGAT+TGG 0.505945 4.3:+31156746 MS.gene031756:CDS
GACCCGACCCGACTTGAAAT+GGG 0.507947 4.3:-31155563 None:intergenic
GAGTGAGGGTCGAGTGGTGA+AGG 0.509192 4.3:-31155615 None:intergenic
AAACGGGTTGAGATGGAAAT+CGG 0.511523 4.3:-31155712 None:intergenic
GGGTCGGGCATGATGAAAAC+GGG 0.514429 4.3:-31155728 None:intergenic
TTTGGAGCCGAACATGGCGT+TGG 0.516288 4.3:+31156682 MS.gene031756:CDS
GCTAGTTTGACGACTCGGGT+TGG 0.518034 4.3:+31156605 MS.gene031756:CDS
AAATTCTTCTTCAACTACTT+GGG 0.520685 4.3:+31156073 MS.gene031756:CDS
CGGAGTCGAAATCGGAGAAA+CGG 0.521347 4.3:-31155647 None:intergenic
CATCATCACCAAAACATCCT+AGG 0.524123 4.3:+31155423 MS.gene031756:CDS
GTTGCGGTGAGAGTCGGGTC+GGG 0.524249 4.3:-31155743 None:intergenic
GGTTGGATTGGGATTTGGAG+TGG 0.534821 4.3:-31155792 None:intergenic
AATTGTTCAAGGGATTCAAT+GGG 0.536168 4.3:+31156226 MS.gene031756:CDS
AGCTCAATAGGCGTAAGAAT+CGG 0.539095 4.3:-31156419 None:intergenic
CGATGTCGTTTCGGTGGTGT+TGG 0.543827 4.3:-31155841 None:intergenic
GATCCGGTTGCGGTGAGAGT+CGG 0.545074 4.3:-31155749 None:intergenic
TTTCGACTCCGATGACTGGA+TGG 0.549581 4.3:+31155659 MS.gene031756:CDS
CAAGAAGAAACAGTCAATAG+AGG 0.549819 4.3:-31156959 None:intergenic
TGAGAGTTTGGAGCCGAACA+TGG 0.552448 4.3:+31156676 MS.gene031756:CDS
GTGGCGGGTAGGAGAAGATC+CGG 0.554130 4.3:-31155765 None:intergenic
ACGAAGCTAGTTTGACGACT+CGG 0.556827 4.3:+31156600 MS.gene031756:CDS
CGACCCATTTCAAGTCGGGT+CGG 0.565104 4.3:+31155560 MS.gene031756:CDS
TAAGGTATCCATCCAGTCAT+CGG 0.565279 4.3:-31155667 None:intergenic
GTTTCATCGATTTCAGCTAC+CGG 0.566724 4.3:+31156344 MS.gene031756:CDS
GAAGTTCCTAAAGCCATAGC+AGG 0.567270 4.3:-31156317 None:intergenic
TCTCTGTCTCACTACATCCA+TGG 0.569966 4.3:+31155460 MS.gene031756:CDS
GGAGTGGCACGGTGGCGGGT+AGG 0.573432 4.3:-31155776 None:intergenic
AAACGGAAGGTGGTTGAGTG+AGG 0.573544 4.3:-31155630 None:intergenic
GGATGGAGGATAAAGAACAG+TGG 0.578860 4.3:+31156780 MS.gene031756:CDS
GGTGAAGGAAAGTTGAAAGC+GGG 0.586484 4.3:-31155600 None:intergenic
TTCGCCAGAGAGGTTTCAAG+TGG 0.588796 4.3:+31156706 MS.gene031756:CDS
AGGAAAGTTGAAAGCGGGAT+CGG 0.590312 4.3:-31155595 None:intergenic
AGAGAGGGTTTGTGATACCT+AGG 0.592419 4.3:-31155440 None:intergenic
GATTGGGATTTGGAGTGGCA+CGG 0.594042 4.3:-31155787 None:intergenic
ATGATGAAAACGGGTTGAGA+TGG 0.594967 4.3:-31155719 None:intergenic
GGAGGATAAAGAACAGTGGA+AGG 0.599058 4.3:+31156784 MS.gene031756:CDS
TGGTGTGAGGGAGAGGATGG+AGG 0.600379 4.3:+31156766 MS.gene031756:CDS
GGATGTAGTGAGACAGAGAG+AGG 0.601119 4.3:-31155456 None:intergenic
GGTTGCGGTGAGAGTCGGGT+CGG 0.604150 4.3:-31155744 None:intergenic
TTCACCGACCCATTTCAAGT+CGG 0.604924 4.3:+31155555 MS.gene031756:CDS
TGATGGTGTGATTGGTGTGA+GGG 0.605734 4.3:+31156754 MS.gene031756:CDS
AACGGAAGGTGGTTGAGTGA+GGG 0.606602 4.3:-31155629 None:intergenic
GTGGTTGAGTGAGGGTCGAG+TGG 0.609203 4.3:-31155621 None:intergenic
GTCAAGCTAAGATATTGTTG+TGG 0.613839 4.3:+31156861 MS.gene031756:CDS
AATGGGTCGGTGAAATCGGT+AGG 0.625917 4.3:-31155546 None:intergenic
GCGATTCTTGAAGCAACAGA+AGG 0.626009 4.3:+31156173 MS.gene031756:CDS
GATGTAGTGAGACAGAGAGA+GGG 0.639502 4.3:-31155455 None:intergenic
GGGTCGGTGAAATCGGTAGG+TGG 0.649301 4.3:-31155543 None:intergenic
GTCGAAATCGGAGAAACGGA+AGG 0.650056 4.3:-31155643 None:intergenic
GCTTACATGTGCGCTGACAG+TGG 0.651171 4.3:+31155312 MS.gene031756:CDS
GAAATCGGAGAAACGGAAGG+TGG 0.658200 4.3:-31155640 None:intergenic
GGTAGGAGAAGATCCGGTTG+CGG 0.659311 4.3:-31155759 None:intergenic
TTGATGGTGTGATTGGTGTG+AGG 0.659575 4.3:+31156753 MS.gene031756:CDS
CGACCCGACTTGAAATGGGT+CGG 0.673110 4.3:-31155559 None:intergenic
TCGGTAGGTGGAAAAGACAA+AGG 0.681946 4.3:-31155531 None:intergenic
TGGGATTTGGAGTGGCACGG+TGG 0.682217 4.3:-31155784 None:intergenic
TGGTGAAGGAAAGTTGAAAG+CGG 0.682780 4.3:-31155601 None:intergenic
TGGCGTTGGATTCGCCAGAG+AGG 0.687403 4.3:+31156696 MS.gene031756:CDS
AAACGACGATGTCGTTTCGG+TGG 0.687537 4.3:-31155847 None:intergenic
GTAGGTGGAAAAGACAAAGG+TGG 0.720227 4.3:-31155528 None:intergenic
GGTTGAGATGGAAATCGGAG+TGG 0.720412 4.3:-31155707 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATATTCATATAGTTGATTT+TGG + chr4.3:31156203-31156222 MS.gene031756:CDS 20.0%
! AAAAGGAAACTCTTTGATTA+GGG - chr4.3:31155998-31156017 None:intergenic 25.0%
! AAATTCTTCTTCAACTACTT+GGG + chr4.3:31156073-31156092 MS.gene031756:CDS 25.0%
! AGCAAATCAATTTCACAAAA+CGG + chr4.3:31155969-31155988 MS.gene031756:CDS 25.0%
! ATCAACTATATGAATATCGT+TGG - chr4.3:31156201-31156220 None:intergenic 25.0%
! TAAAAGGAAACTCTTTGATT+AGG - chr4.3:31155999-31156018 None:intergenic 25.0%
!!! AATCCTAAGATTGTTACTTT+AGG + chr4.3:31156572-31156591 MS.gene031756:CDS 25.0%
!!! TTGATTTTGGAATTGTTCAA+GGG + chr4.3:31156216-31156235 MS.gene031756:CDS 25.0%
AAACTCAGATAAACGATTAC+CGG - chr4.3:31156366-31156385 None:intergenic 30.0%
AAATGTGCGAACTTTGAATA+TGG - chr4.3:31156140-31156159 None:intergenic 30.0%
AATGTGCGAACTTTGAATAT+GGG - chr4.3:31156139-31156158 None:intergenic 30.0%
AATTGTTCAAGGGATTCAAT+GGG + chr4.3:31156226-31156245 MS.gene031756:CDS 30.0%
CAAATTCTTCTTCAACTACT+TGG + chr4.3:31156072-31156091 MS.gene031756:CDS 30.0%
CCAGAAATTCTTACACTATT+AGG - chr4.3:31156293-31156312 None:intergenic 30.0%
CCTAATAGTGTAAGAATTTC+TGG + chr4.3:31156290-31156309 MS.gene031756:CDS 30.0%
TAAGGCTTGTGAAAAGTAAA+AGG - chr4.3:31156015-31156034 None:intergenic 30.0%
TCACCTAAAGTAACAATCTT+AGG - chr4.3:31156578-31156597 None:intergenic 30.0%
! CTTTTGTATTCAACCAAATG+AGG + chr4.3:31156457-31156476 MS.gene031756:CDS 30.0%
! GCTTCAGTTGTATAATCTTT+TGG + chr4.3:31156499-31156518 MS.gene031756:CDS 30.0%
!! AATTGCTTGATTTGGTTTGT+GGG - chr4.3:31155931-31155950 None:intergenic 30.0%
!! TGAAATTGATTTGCTGAGAT+GGG - chr4.3:31155964-31155983 None:intergenic 30.0%
!!! GTTGATTTTGGAATTGTTCA+AGG + chr4.3:31156215-31156234 MS.gene031756:CDS 30.0%
!!! GTTGTTCTTGTTGTTGTTTT+TGG - chr4.3:31155371-31155390 None:intergenic 30.0%
AGGGTTTCAATTGCTTGATT+TGG - chr4.3:31155939-31155958 None:intergenic 35.0%
AGTGATGAAGAAGAAGATAC+TGG - chr4.3:31155507-31155526 None:intergenic 35.0%
CAAGAAGAAACAGTCAATAG+AGG - chr4.3:31156962-31156981 None:intergenic 35.0%
GAATTGTTCAAGGGATTCAA+TGG + chr4.3:31156225-31156244 MS.gene031756:CDS 35.0%
GATTCATCAAGAAGCTCAAT+AGG - chr4.3:31156434-31156453 None:intergenic 35.0%
GTCAAGCTAAGATATTGTTG+TGG + chr4.3:31156861-31156880 MS.gene031756:CDS 35.0%
TTAACAGCTAAAGCCTCATT+TGG - chr4.3:31156473-31156492 None:intergenic 35.0%
TTCGATGAGTGAAGCAATTT+CGG - chr4.3:31155907-31155926 None:intergenic 35.0%
! AGATTGTGCAGTGATTTTGT+TGG - chr4.3:31156039-31156058 None:intergenic 35.0%
! CTCTATTGACTGTTTCTTCT+TGG + chr4.3:31156960-31156979 MS.gene031756:CDS 35.0%
! GATGATGATGTTGATGTTGT+TGG - chr4.3:31155410-31155429 None:intergenic 35.0%
! TTGTGATACCTAGGATGTTT+TGG - chr4.3:31155434-31155453 None:intergenic 35.0%
!! AAGGTTTTGATGGAAAGTTG+TGG + chr4.3:31156803-31156822 MS.gene031756:CDS 35.0%
!! AATTTCTGGTATACCTGCTA+TGG + chr4.3:31156304-31156323 MS.gene031756:CDS 35.0%
!! CAATTGCTTGATTTGGTTTG+TGG - chr4.3:31155932-31155951 None:intergenic 35.0%
!! GTGAAATTGATTTGCTGAGA+TGG - chr4.3:31155965-31155984 None:intergenic 35.0%
!! TGCAGTGATTTTGTTGGTTA+AGG - chr4.3:31156033-31156052 None:intergenic 35.0%
!!! AAGTTGTGGTTTTGAATCTG+TGG + chr4.3:31156817-31156836 MS.gene031756:CDS 35.0%
!!! AGTTGTGGTTTTGAATCTGT+GGG + chr4.3:31156818-31156837 MS.gene031756:CDS 35.0%
!!! TCAACCTGCTTTTTTGTCTT+TGG + chr4.3:31156922-31156941 MS.gene031756:CDS 35.0%
!!! TGCTGCTTTTTTTGAGAGTT+TGG + chr4.3:31156664-31156683 MS.gene031756:CDS 35.0%
AGCTCAATAGGCGTAAGAAT+CGG - chr4.3:31156422-31156441 None:intergenic 40.0%
ATATGGGCATGCATCGTTTA+AGG - chr4.3:31156123-31156142 None:intergenic 40.0%
CAAGCCAAAGACAAAAAAGC+AGG - chr4.3:31156929-31156948 None:intergenic 40.0%
CATCATCACCAAAACATCCT+AGG + chr4.3:31155423-31155442 MS.gene031756:CDS 40.0%
CTTGTTGAGCTATTGCCATT+AGG - chr4.3:31155341-31155360 None:intergenic 40.0%
GAGTGAAGCAATTTCGGTTA+GGG - chr4.3:31155901-31155920 None:intergenic 40.0%
GTAGCTGAAATCGATGAAAC+TGG - chr4.3:31156344-31156363 None:intergenic 40.0%
TGAGTGAAGCAATTTCGGTT+AGG - chr4.3:31155902-31155921 None:intergenic 40.0%
TGGTGAAGGAAAGTTGAAAG+CGG - chr4.3:31155604-31155623 None:intergenic 40.0%
TTGAAATGGGTCGGTGAAAT+CGG - chr4.3:31155553-31155572 None:intergenic 40.0%
TTGCTTGGTAGGAGAATTGA+TGG + chr4.3:31156737-31156756 MS.gene031756:CDS 40.0%
! TAAGGTATCCATCCAGTCAT+CGG - chr4.3:31155670-31155689 None:intergenic 40.0%
! TGAAAACGACGATGTCGTTT+CGG - chr4.3:31155853-31155872 None:intergenic 40.0%
! TTGTTGTTGTTGTTGTGCCA+TGG - chr4.3:31155480-31155499 None:intergenic 40.0%
!! AAACGGGTTGAGATGGAAAT+CGG - chr4.3:31155715-31155734 None:intergenic 40.0%
!! AGAACAGTGGAAGGTTTTGA+TGG + chr4.3:31156793-31156812 MS.gene031756:CDS 40.0%
!! AGGAGAATTGATGGTGTGAT+TGG + chr4.3:31156746-31156765 MS.gene031756:CDS 40.0%
!! ATGATGAAAACGGGTTGAGA+TGG - chr4.3:31155722-31155741 None:intergenic 40.0%
!! GTTTCATCGATTTCAGCTAC+CGG + chr4.3:31156344-31156363 MS.gene031756:CDS 40.0%
!! TGATTTGCTGAGATGGGTTA+GGG - chr4.3:31155958-31155977 None:intergenic 40.0%
!! TTGATTTGCTGAGATGGGTT+AGG - chr4.3:31155959-31155978 None:intergenic 40.0%
!!! CAAACGAAGCGCTTTTTCAA+CGG - chr4.3:31156540-31156559 None:intergenic 40.0%
!!! CGGTTAGGGTTTTAAGAAGT+GGG - chr4.3:31155887-31155906 None:intergenic 40.0%
!!! CTGCTTTTTTGTCTTTGGCT+TGG + chr4.3:31156927-31156946 MS.gene031756:CDS 40.0%
!!! GGTTTTAAGAAGTGGGTTTG+TGG - chr4.3:31155880-31155899 None:intergenic 40.0%
!!! TCGGTTAGGGTTTTAAGAAG+TGG - chr4.3:31155888-31155907 None:intergenic 40.0%
AGAGAGGGTTTGTGATACCT+AGG - chr4.3:31155443-31155462 None:intergenic 45.0%
AGGAAAGTTGAAAGCGGGAT+CGG - chr4.3:31155598-31155617 None:intergenic 45.0%
ATGGAAATCGGAGTGGTTGT+CGG - chr4.3:31155703-31155722 None:intergenic 45.0%
GAAGTTCCTAAAGCCATAGC+AGG - chr4.3:31156320-31156339 None:intergenic 45.0%
GATGTAGTGAGACAGAGAGA+GGG - chr4.3:31155458-31155477 None:intergenic 45.0%
GCATTTGCAACTCGTTCTTC+TGG + chr4.3:31156263-31156282 MS.gene031756:CDS 45.0%
GCGATTCTTGAAGCAACAGA+AGG + chr4.3:31156173-31156192 MS.gene031756:CDS 45.0%
GGAGGATAAAGAACAGTGGA+AGG + chr4.3:31156784-31156803 MS.gene031756:CDS 45.0%
GGATGGAGGATAAAGAACAG+TGG + chr4.3:31156780-31156799 MS.gene031756:CDS 45.0%
GGTATACCTGCTATGGCTTT+AGG + chr4.3:31156311-31156330 MS.gene031756:CDS 45.0%
GGTGAAGGAAAGTTGAAAGC+GGG - chr4.3:31155603-31155622 None:intergenic 45.0%
GTAGGTGGAAAAGACAAAGG+TGG - chr4.3:31155531-31155550 None:intergenic 45.0%
TCGGTAGGTGGAAAAGACAA+AGG - chr4.3:31155534-31155553 None:intergenic 45.0%
TCTCTGTCTCACTACATCCA+TGG + chr4.3:31155460-31155479 MS.gene031756:CDS 45.0%
! ACGAAGCTAGTTTGACGACT+CGG + chr4.3:31156600-31156619 MS.gene031756:CDS 45.0%
! CAAGTGGAGAGTCTTTTGCT+TGG + chr4.3:31156722-31156741 MS.gene031756:CDS 45.0%
! TGATGGTGTGATTGGTGTGA+GGG + chr4.3:31156754-31156773 MS.gene031756:CDS 45.0%
! TGGAGAGTCTTTTGCTTGGT+AGG + chr4.3:31156726-31156745 MS.gene031756:CDS 45.0%
! TTCACCGACCCATTTCAAGT+CGG + chr4.3:31155555-31155574 MS.gene031756:CDS 45.0%
!! CGTTGAAAAAGCGCTTCGTT+TGG + chr4.3:31156538-31156557 MS.gene031756:CDS 45.0%
!! TTGATGGTGTGATTGGTGTG+AGG + chr4.3:31156753-31156772 MS.gene031756:CDS 45.0%
!!! TTGGTGTCGTTTTTGCGTTG+CGG - chr4.3:31155825-31155844 None:intergenic 45.0%
AAACGGAAGGTGGTTGAGTG+AGG - chr4.3:31155633-31155652 None:intergenic 50.0%
AACGGAAGGTGGTTGAGTGA+GGG - chr4.3:31155632-31155651 None:intergenic 50.0%
AATGGGTCGGTGAAATCGGT+AGG - chr4.3:31155549-31155568 None:intergenic 50.0%
CCAGTCATCGGAGTCGAAAT+CGG - chr4.3:31155658-31155677 None:intergenic 50.0%
CGCTGACAGTGGAAACCTAA+TGG + chr4.3:31155323-31155342 MS.gene031756:CDS 50.0%
CGGAGTCGAAATCGGAGAAA+CGG - chr4.3:31155650-31155669 None:intergenic 50.0%
CTCTCCACTTGAAACCTCTC+TGG - chr4.3:31156713-31156732 None:intergenic 50.0%
GAAATCGGAGAAACGGAAGG+TGG - chr4.3:31155643-31155662 None:intergenic 50.0%
GGATGTAGTGAGACAGAGAG+AGG - chr4.3:31155459-31155478 None:intergenic 50.0%
GGTTGAGATGGAAATCGGAG+TGG - chr4.3:31155710-31155729 None:intergenic 50.0%
GTCGAAATCGGAGAAACGGA+AGG - chr4.3:31155646-31155665 None:intergenic 50.0%
TTCGCCAGAGAGGTTTCAAG+TGG + chr4.3:31156706-31156725 MS.gene031756:CDS 50.0%
TTTCGACTCCGATGACTGGA+TGG + chr4.3:31155659-31155678 MS.gene031756:CDS 50.0%
! AAACGACGATGTCGTTTCGG+TGG - chr4.3:31155850-31155869 None:intergenic 50.0%
! CGAAGCTAGTTTGACGACTC+GGG + chr4.3:31156601-31156620 MS.gene031756:CDS 50.0%
! GATTGGGATTTGGAGTGGCA+CGG - chr4.3:31155790-31155809 None:intergenic 50.0%
! GGTTGGATTGGGATTTGGAG+TGG - chr4.3:31155795-31155814 None:intergenic 50.0%
! TCACCGACCCATTTCAAGTC+GGG + chr4.3:31155556-31155575 MS.gene031756:CDS 50.0%
! TGAGAGTTTGGAGCCGAACA+TGG + chr4.3:31156676-31156695 MS.gene031756:CDS 50.0%
! TGGGTGGTTGGATTGGGATT+TGG - chr4.3:31155800-31155819 None:intergenic 50.0%
!! AAAGCGGGATCGGTGTTTTC+AGG - chr4.3:31155588-31155607 None:intergenic 50.0%
!! TCGTTTTTGCGTTGCGGAGT+GGG - chr4.3:31155819-31155838 None:intergenic 50.0%
CCGATTTCGACTCCGATGAC+TGG + chr4.3:31155655-31155674 MS.gene031756:CDS 55.0%
CGGGTCGGGCATGATGAAAA+CGG - chr4.3:31155732-31155751 None:intergenic 55.0%
GATTGGTGTGAGGGAGAGGA+TGG + chr4.3:31156763-31156782 MS.gene031756:CDS 55.0%
GGCGAATCCAACGCCATGTT+CGG - chr4.3:31156692-31156711 None:intergenic 55.0%
GGGTCGGGCATGATGAAAAC+GGG - chr4.3:31155731-31155750 None:intergenic 55.0%
GGTAGGAGAAGATCCGGTTG+CGG - chr4.3:31155762-31155781 None:intergenic 55.0%
GTGTGATTGGTGTGAGGGAG+AGG + chr4.3:31156759-31156778 MS.gene031756:CDS 55.0%
GTTGTCGGCAGCGGACATTA+AGG - chr4.3:31155688-31155707 None:intergenic 55.0%
! CGACCCATTTCAAGTCGGGT+CGG + chr4.3:31155560-31155579 MS.gene031756:CDS 55.0%
! CGACCCGACTTGAAATGGGT+CGG - chr4.3:31155562-31155581 None:intergenic 55.0%
! CGATGTCGTTTCGGTGGTGT+TGG - chr4.3:31155844-31155863 None:intergenic 55.0%
! GACCCATTTCAAGTCGGGTC+GGG + chr4.3:31155561-31155580 MS.gene031756:CDS 55.0%
! GACCCGACCCGACTTGAAAT+GGG - chr4.3:31155566-31155585 None:intergenic 55.0%
! GCTAGTTTGACGACTCGGGT+TGG + chr4.3:31156605-31156624 MS.gene031756:CDS 55.0%
! GCTTACATGTGCGCTGACAG+TGG + chr4.3:31155312-31155331 MS.gene031756:CDS 55.0%
! TTTGGAGCCGAACATGGCGT+TGG + chr4.3:31156682-31156701 MS.gene031756:CDS 55.0%
! TTTTTGCGTTGCGGAGTGGG+TGG - chr4.3:31155816-31155835 None:intergenic 55.0%
!!! GTCGTTTTTGCGTTGCGGAG+TGG - chr4.3:31155820-31155839 None:intergenic 55.0%
ATCCGGTTGCGGTGAGAGTC+GGG - chr4.3:31155751-31155770 None:intergenic 60.0%
ATCGGAGTGGTTGTCGGCAG+CGG - chr4.3:31155697-31155716 None:intergenic 60.0%
GAGTGAGGGTCGAGTGGTGA+AGG - chr4.3:31155618-31155637 None:intergenic 60.0%
GATCCGGTTGCGGTGAGAGT+CGG - chr4.3:31155752-31155771 None:intergenic 60.0%
GCGGAGTGGGTGGTTGGATT+GGG - chr4.3:31155806-31155825 None:intergenic 60.0%
GGGTCGGTGAAATCGGTAGG+TGG - chr4.3:31155546-31155565 None:intergenic 60.0%
GTGGTTGAGTGAGGGTCGAG+TGG - chr4.3:31155624-31155643 None:intergenic 60.0%
TGCGGAGTGGGTGGTTGGAT+TGG - chr4.3:31155807-31155826 None:intergenic 60.0%
TGGTGTGAGGGAGAGGATGG+AGG + chr4.3:31156766-31156785 MS.gene031756:CDS 60.0%
! ATTTGGAGTGGCACGGTGGC+GGG - chr4.3:31155783-31155802 None:intergenic 60.0%
! GATTTGGAGTGGCACGGTGG+CGG - chr4.3:31155784-31155803 None:intergenic 60.0%
! GGACCCGACCCGACTTGAAA+TGG - chr4.3:31155567-31155586 None:intergenic 60.0%
! TGGCGTTGGATTCGCCAGAG+AGG + chr4.3:31156696-31156715 MS.gene031756:CDS 60.0%
! TGGGATTTGGAGTGGCACGG+TGG - chr4.3:31155787-31155806 None:intergenic 60.0%
!! GTGGCGGGTAGGAGAAGATC+CGG - chr4.3:31155768-31155787 None:intergenic 60.0%
GACCCGACTCTCACCGCAAC+CGG + chr4.3:31155746-31155765 MS.gene031756:CDS 65.0%
GGTTGCGGTGAGAGTCGGGT+CGG - chr4.3:31155747-31155766 None:intergenic 65.0%
GTTGCGGTGAGAGTCGGGTC+GGG - chr4.3:31155746-31155765 None:intergenic 65.0%
TGCGTTGCGGAGTGGGTGGT+TGG - chr4.3:31155812-31155831 None:intergenic 65.0%
!! GGAGTGGCACGGTGGCGGGT+AGG - chr4.3:31155779-31155798 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 31155309 31156988 31155309 ID=MS.gene031756
chr4.3 mRNA 31155309 31156988 31155309 ID=MS.gene031756.t1;Parent=MS.gene031756
chr4.3 exon 31155309 31156988 31155309 ID=MS.gene031756.t1.exon1;Parent=MS.gene031756.t1
chr4.3 CDS 31155309 31156988 31155309 ID=cds.MS.gene031756.t1;Parent=MS.gene031756.t1
Gene Sequence

>MS.gene031756

ATGGCTTACATGTGCGCTGACAGTGGAAACCTAATGGCAATAGCTCAACAAGTCATCAACCAAAAACAACAACAAGAACAACATGAACAACAGCAACACCAACAACATCAACATCATCATCACCAAAACATCCTAGGTATCACAAACCCTCTCTCTGTCTCACTACATCCATGGCACAACAACAACAACAACATTCCAGTATCTTCTTCTTCATCACTACCACCTTTGTCTTTTCCACCTACCGATTTCACCGACCCATTTCAAGTCGGGTCGGGTCCTGAAAACACCGATCCCGCTTTCAACTTTCCTTCACCACTCGACCCTCACTCAACCACCTTCCGTTTCTCCGATTTCGACTCCGATGACTGGATGGATACCTTAATGTCCGCTGCCGACAACCACTCCGATTTCCATCTCAACCCGTTTTCATCATGCCCGACCCGACTCTCACCGCAACCGGATCTTCTCCTACCCGCCACCGTGCCACTCCAAATCCCAATCCAACCACCCACTCCGCAACGCAAAAACGACACCAACACCACCGAAACGACATCGTCGTTTTCATCTTCCACAAACCCACTTCTTAAAACCCTAACCGAAATTGCTTCACTCATCGAAACCCACAAACCAAATCAAGCAATTGAAACCCTAACCCATCTCAGCAAATCAATTTCACAAAACGGAAACCCTAATCAAAGAGTTTCCTTTTACTTTTCACAAGCCTTAACCAACAAAATCACTGCACAATCTTCAATAGCTTCTTCAAATTCTTCTTCAACTACTTGGGAAGAGTTAACACTCTCTTACAAAGCCTTAAACGATGCATGCCCATATTCAAAGTTCGCACATTTAACAGCAAATCAAGCGATTCTTGAAGCAACAGAAGGATCCAACGATATTCATATAGTTGATTTTGGAATTGTTCAAGGGATTCAATGGGCTGCATTGTTACAAGCATTTGCAACTCGTTCTTCTGGTAAACCTAATAGTGTAAGAATTTCTGGTATACCTGCTATGGCTTTAGGAACTTCACCAGTTTCATCGATTTCAGCTACCGGTAATCGTTTATCTGAGTTTGCGAAACTTCTAGAGTTAAATTTTGAGTTTACACCGATTCTTACGCCTATTGAGCTTCTTGATGAATCAAGCTTTTGTATTCAACCAAATGAGGCTTTAGCTGTTAATTTCATGCTTCAGTTGTATAATCTTTTGGATGAGAATATGAATTCCGTTGAAAAAGCGCTTCGTTTGGCGAAATCATTGAATCCTAAGATTGTTACTTTAGGTGAATACGAAGCTAGTTTGACGACTCGGGTTGGATTTGTTGAAAGATTTGAAACTGCTTTTAATTATTTTGCTGCTTTTTTTGAGAGTTTGGAGCCGAACATGGCGTTGGATTCGCCAGAGAGGTTTCAAGTGGAGAGTCTTTTGCTTGGTAGGAGAATTGATGGTGTGATTGGTGTGAGGGAGAGGATGGAGGATAAAGAACAGTGGAAGGTTTTGATGGAAAGTTGTGGTTTTGAATCTGTGGGTTTGAGTCATTATGCAATTAGTCAAGCTAAGATATTGTTGTGGAATTATAGTTATAGTTCTTTGTATTCTCTTGTTGAATCTCAACCTGCTTTTTTGTCTTTGGCTTGGAAAGATGTTCCTCTATTGACTGTTTCTTCTTGGCGTTGA
Protein sequence

>MS.gene031756.t1

MAYMCADSGNLMAIAQQVINQKQQQEQHEQQQHQQHQHHHHQNILGITNPLSVSLHPWHNNNNNIPVSSSSSLPPLSFPPTDFTDPFQVGSGPENTDPAFNFPSPLDPHSTTFRFSDFDSDDWMDTLMSAADNHSDFHLNPFSSCPTRLSPQPDLLLPATVPLQIPIQPPTPQRKNDTNTTETTSSFSSSTNPLLKTLTEIASLIETHKPNQAIETLTHLSKSISQNGNPNQRVSFYFSQALTNKITAQSSIASSNSSSTTWEELTLSYKALNDACPYSKFAHLTANQAILEATEGSNDIHIVDFGIVQGIQWAALLQAFATRSSGKPNSVRISGIPAMALGTSPVSSISATGNRLSEFAKLLELNFEFTPILTPIELLDESSFCIQPNEALAVNFMLQLYNLLDENMNSVEKALRLAKSLNPKIVTLGEYEASLTTRVGFVERFETAFNYFAAFFESLEPNMALDSPERFQVESLLLGRRIDGVIGVRERMEDKEQWKVLMESCGFESVGLSHYAISQAKILLWNYSYSSLYSLVESQPAFLSLAWKDVPLLTVSSWR