Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031788.t1 | AES63738.2 | 85.2 | 122 | 18 | 0 | 1 | 122 | 1 | 122 | 2.00E-55 | 224.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031788.t1 | G7INX6 | 85.2 | 122 | 18 | 0 | 1 | 122 | 1 | 122 | 1.4e-55 | 224.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene031788.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031788.t1 | MTR_2g012700 | 85.246 | 122 | 18 | 0 | 1 | 122 | 1 | 122 | 5.08e-75 | 218 |
| MS.gene031788.t1 | MTR_2g062330 | 81.667 | 120 | 21 | 1 | 3 | 122 | 23 | 141 | 3.51e-68 | 202 |
| MS.gene031788.t1 | MTR_7g117590 | 35.385 | 130 | 65 | 4 | 7 | 119 | 44 | 171 | 6.47e-21 | 82.8 |
| MS.gene031788.t1 | MTR_7g117585 | 36.634 | 101 | 62 | 1 | 18 | 116 | 42 | 142 | 4.90e-18 | 74.7 |
| MS.gene031788.t1 | MTR_0010s0310 | 41.758 | 91 | 51 | 1 | 22 | 110 | 150 | 240 | 1.28e-17 | 75.9 |
| MS.gene031788.t1 | MTR_3g008190 | 29.897 | 97 | 68 | 0 | 24 | 120 | 6 | 102 | 1.59e-16 | 69.7 |
| MS.gene031788.t1 | MTR_5g071410 | 34.653 | 101 | 64 | 1 | 18 | 116 | 165 | 265 | 3.58e-15 | 69.7 |
| MS.gene031788.t1 | MTR_6g477950 | 37.037 | 81 | 51 | 0 | 39 | 119 | 64 | 144 | 1.10e-13 | 65.9 |
| MS.gene031788.t1 | MTR_0010s0320 | 37.363 | 91 | 53 | 2 | 24 | 110 | 192 | 282 | 7.13e-13 | 63.5 |
| MS.gene031788.t1 | MTR_6g087720 | 33.721 | 86 | 56 | 1 | 24 | 108 | 214 | 299 | 1.10e-12 | 63.2 |
| MS.gene031788.t1 | MTR_0010s0290 | 37.363 | 91 | 53 | 2 | 24 | 110 | 223 | 313 | 1.37e-12 | 63.2 |
| MS.gene031788.t1 | MTR_5g078270 | 34.066 | 91 | 58 | 1 | 22 | 110 | 208 | 298 | 2.06e-12 | 62.4 |
| MS.gene031788.t1 | MTR_8g069780 | 25.843 | 89 | 66 | 0 | 31 | 119 | 67 | 155 | 8.62e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATGTTACTGCTTCTATTT+TGG | 0.123735 | 4.3:-31522753 | MS.gene031788:CDS |
| AAAATTCCGATGTTCATTAT+TGG | 0.199581 | 4.3:-31522781 | MS.gene031788:CDS |
| GAAGTCAATGGTGCTGAATT+AGG | 0.200131 | 4.3:-31522812 | MS.gene031788:CDS |
| TACCAGTTTCTTCATCAAAA+AGG | 0.252196 | 4.3:+31522562 | None:intergenic |
| TGTATGCTACCGTCGTTAAC+TGG | 0.297739 | 4.3:-31522395 | MS.gene031788:CDS |
| CCTCGACGTGTTGCCGAGAA+TGG | 0.307864 | 4.3:-31522612 | MS.gene031788:CDS |
| CGGTCAATAATCCTCCTAAT+AGG | 0.355854 | 4.3:-31522419 | MS.gene031788:CDS |
| TTTGGATGGAAACAATGTTT+TGG | 0.356448 | 4.3:-31522735 | MS.gene031788:intron |
| AAGTCAATGGTGCTGAATTA+GGG | 0.416476 | 4.3:-31522811 | MS.gene031788:CDS |
| ACGGTAGCATACATCCTATT+AGG | 0.440754 | 4.3:+31522405 | None:intergenic |
| ATTGGTATACAAACCATTCT+CGG | 0.470714 | 4.3:+31522599 | None:intergenic |
| GTATGCTACCGTCGTTAACT+GGG | 0.522932 | 4.3:-31522394 | MS.gene031788:CDS |
| GCATGGATCAGAAATACATA+GGG | 0.559318 | 4.3:-31522503 | MS.gene031788:CDS |
| AGCATGGATCAGAAATACAT+AGG | 0.564126 | 4.3:-31522504 | MS.gene031788:CDS |
| CTTGAAAAGTGCCTAAAAGT+TGG | 0.570138 | 4.3:-31522459 | MS.gene031788:CDS |
| AGAAATACATAGGGAGAGGA+TGG | 0.573439 | 4.3:-31522494 | MS.gene031788:CDS |
| GATCAGAAATACATAGGGAG+AGG | 0.587262 | 4.3:-31522498 | MS.gene031788:CDS |
| TCACACTGCGTCAAGAAGCA+TGG | 0.587686 | 4.3:-31522520 | MS.gene031788:CDS |
| CCATTCTCGGCAACACGTCG+AGG | 0.599354 | 4.3:+31522612 | None:intergenic |
| GAGATTGGAGTTGAAGTCAA+TGG | 0.619642 | 4.3:-31522824 | MS.gene031788:CDS |
| GTAGCATACATCCTATTAGG+AGG | 0.633251 | 4.3:+31522408 | None:intergenic |
| ATACTTCCAATAATGAACAT+CGG | 0.646998 | 4.3:+31522775 | None:intergenic |
| TAATCAGTCCCAGTTAACGA+CGG | 0.733495 | 4.3:+31522386 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATTCCGATGTTCATTAT+TGG | - | chr4.3:31522438-31522457 | MS.gene031788:CDS | 25.0% |
| ! | ATACTTCCAATAATGAACAT+CGG | + | chr4.3:31522447-31522466 | None:intergenic | 25.0% |
| !! | AAGGAGAATTTTAGATTGAT+TGG | + | chr4.3:31522641-31522660 | None:intergenic | 25.0% |
| !! | TAATGTTACTGCTTCTATTT+TGG | - | chr4.3:31522466-31522485 | MS.gene031788:CDS | 25.0% |
| ATTGGTATACAAACCATTCT+CGG | + | chr4.3:31522623-31522642 | None:intergenic | 30.0% | |
| TACCAGTTTCTTCATCAAAA+AGG | + | chr4.3:31522660-31522679 | None:intergenic | 30.0% | |
| ! | AAACAACATCACCAACTTTT+AGG | + | chr4.3:31522774-31522793 | None:intergenic | 30.0% |
| ! | TTTGGATGGAAACAATGTTT+TGG | - | chr4.3:31522484-31522503 | MS.gene031788:CDS | 30.0% |
| AGCATGGATCAGAAATACAT+AGG | - | chr4.3:31522715-31522734 | MS.gene031788:intron | 35.0% | |
| GCATGGATCAGAAATACATA+GGG | - | chr4.3:31522716-31522735 | MS.gene031788:intron | 35.0% | |
| ! | AAGTCAATGGTGCTGAATTA+GGG | - | chr4.3:31522408-31522427 | MS.gene031788:CDS | 35.0% |
| ! | CTCCTTTTTGATGAAGAAAC+TGG | - | chr4.3:31522655-31522674 | MS.gene031788:intron | 35.0% |
| !! | CTTGAAAAGTGCCTAAAAGT+TGG | - | chr4.3:31522760-31522779 | MS.gene031788:CDS | 35.0% |
| !! | GTTACTGCTTCTATTTTGGA+TGG | - | chr4.3:31522470-31522489 | MS.gene031788:CDS | 35.0% |
| !!! | GGAAACAATGTTTTGGTTAG+TGG | - | chr4.3:31522491-31522510 | MS.gene031788:CDS | 35.0% |
| !!! | TGGTGATGTTGTTTTGTTCA+CGG | - | chr4.3:31522780-31522799 | MS.gene031788:CDS | 35.0% |
| ACGGTAGCATACATCCTATT+AGG | + | chr4.3:31522817-31522836 | None:intergenic | 40.0% | |
| AGAAATACATAGGGAGAGGA+TGG | - | chr4.3:31522725-31522744 | MS.gene031788:intron | 40.0% | |
| CGGTCAATAATCCTCCTAAT+AGG | - | chr4.3:31522800-31522819 | MS.gene031788:CDS | 40.0% | |
| GAGATTGGAGTTGAAGTCAA+TGG | - | chr4.3:31522395-31522414 | MS.gene031788:CDS | 40.0% | |
| GATCAGAAATACATAGGGAG+AGG | - | chr4.3:31522721-31522740 | MS.gene031788:intron | 40.0% | |
| GTAGCATACATCCTATTAGG+AGG | + | chr4.3:31522814-31522833 | None:intergenic | 40.0% | |
| ! | GAAGTCAATGGTGCTGAATT+AGG | - | chr4.3:31522407-31522426 | MS.gene031788:CDS | 40.0% |
| GTATGCTACCGTCGTTAACT+GGG | - | chr4.3:31522825-31522844 | MS.gene031788:CDS | 45.0% | |
| TGTATGCTACCGTCGTTAAC+TGG | - | chr4.3:31522824-31522843 | MS.gene031788:CDS | 45.0% | |
| TCACACTGCGTCAAGAAGCA+TGG | - | chr4.3:31522699-31522718 | MS.gene031788:intron | 50.0% | |
| CCATTCTCGGCAACACGTCG+AGG | + | chr4.3:31522610-31522629 | None:intergenic | 60.0% | |
| CCTCGACGTGTTGCCGAGAA+TGG | - | chr4.3:31522607-31522626 | MS.gene031788:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 31522389 | 31522852 | 31522389 | ID=MS.gene031788 |
| chr4.3 | mRNA | 31522389 | 31522852 | 31522389 | ID=MS.gene031788.t1;Parent=MS.gene031788 |
| chr4.3 | exon | 31522736 | 31522852 | 31522736 | ID=MS.gene031788.t1.exon1;Parent=MS.gene031788.t1 |
| chr4.3 | CDS | 31522736 | 31522852 | 31522736 | ID=cds.MS.gene031788.t1;Parent=MS.gene031788.t1 |
| chr4.3 | exon | 31522389 | 31522640 | 31522389 | ID=MS.gene031788.t1.exon2;Parent=MS.gene031788.t1 |
| chr4.3 | CDS | 31522389 | 31522640 | 31522389 | ID=cds.MS.gene031788.t1;Parent=MS.gene031788.t1 |
| Gene Sequence |
| Protein sequence |
