AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031833

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031833.t1 MTR_4g076140 96.186 472 18 0 7 478 1 472 0.0 917
MS.gene031833.t1 MTR_5g009080 74.583 480 114 6 6 478 3 481 0.0 732
MS.gene031833.t1 MTR_3g022830 49.888 445 213 4 33 477 4 438 7.02e-161 462
MS.gene031833.t1 MTR_7g027190 34.000 450 215 6 43 475 289 673 5.76e-75 249
MS.gene031833.t1 MTR_5g097480 31.090 431 231 6 51 475 174 544 1.23e-65 221
MS.gene031833.t1 MTR_1g106590 58.228 158 57 1 8 165 93 241 9.08e-56 189
MS.gene031833.t1 MTR_1g106590 67.778 90 28 1 289 378 251 339 9.75e-24 102
MS.gene031833.t1 MTR_1g029420 29.604 429 236 8 53 475 224 592 1.40e-55 195
MS.gene031833.t1 MTR_2g082090 31.163 430 230 8 51 474 209 578 4.63e-55 193
MS.gene031833.t1 MTR_3g089055 28.904 429 239 8 53 475 202 570 1.14e-53 189
MS.gene031833.t1 MTR_3g089055 28.904 429 239 8 53 475 197 565 1.25e-53 189
MS.gene031833.t1 MTR_3g056110 27.703 444 254 8 38 475 160 542 4.33e-52 184
MS.gene031833.t1 MTR_4g104020 28.604 444 235 11 48 475 143 520 6.86e-50 178
MS.gene031833.t1 MTR_4g064160 27.739 429 241 7 54 474 316 683 2.75e-48 176
MS.gene031833.t1 MTR_7g062120 27.336 428 246 5 54 475 372 740 1.88e-47 175
MS.gene031833.t1 MTR_4g064120 26.711 453 263 8 27 470 229 621 9.86e-46 169
MS.gene031833.t1 MTR_4g064180 26.490 453 264 8 27 470 229 621 8.31e-44 163
MS.gene031833.t1 MTR_2g026250 27.336 428 244 9 55 475 229 596 1.06e-43 162
MS.gene031833.t1 MTR_4g064150 26.957 460 262 11 26 474 336 732 1.28e-43 164
MS.gene031833.t1 MTR_3g022580 39.524 210 86 6 269 478 18 186 2.43e-43 151
MS.gene031833.t1 MTR_2g097410 25.935 428 252 5 54 474 371 740 2.95e-43 163
MS.gene031833.t1 MTR_2g097467 26.147 436 236 8 54 474 289 653 1.12e-40 155
MS.gene031833.t1 MTR_2g097473 26.230 427 247 7 54 474 289 653 6.02e-40 152
MS.gene031833.t1 MTR_2g097390 25.858 437 259 9 43 474 264 640 9.81e-40 152
MS.gene031833.t1 MTR_2g097350 26.087 437 258 8 43 474 263 639 4.60e-39 150
MS.gene031833.t1 MTR_8g442410 44.667 150 81 1 326 475 378 525 6.03e-39 148
MS.gene031833.t1 MTR_8g442410 31.855 248 144 7 47 272 148 392 4.15e-25 108
MS.gene031833.t1 MTR_4g102790 35.814 215 134 1 47 257 285 499 6.83e-39 150
MS.gene031833.t1 MTR_4g102790 31.373 153 98 5 328 474 510 661 2.77e-18 88.2
MS.gene031833.t1 MTR_2g034280 29.083 447 236 15 45 475 194 575 9.25e-38 145
MS.gene031833.t1 MTR_7g074650 39.791 191 112 2 58 247 442 630 1.35e-36 144
MS.gene031833.t1 MTR_7g074650 36.913 149 89 3 330 475 646 792 6.96e-20 93.6
MS.gene031833.t1 MTR_2g097463 25.995 427 248 7 54 474 290 654 1.76e-36 142
MS.gene031833.t1 MTR_1g069725 27.918 437 238 12 46 477 101 465 3.59e-36 139
MS.gene031833.t1 MTR_1g096030 28.228 457 230 16 48 476 74 460 2.43e-35 137
MS.gene031833.t1 MTR_3g065980 34.234 222 132 4 42 258 160 372 7.34e-34 134
MS.gene031833.t1 MTR_3g065980 35.758 165 93 2 324 475 376 540 3.88e-28 117
MS.gene031833.t1 MTR_2g034250 26.386 451 246 11 45 475 201 585 1.42e-32 131
MS.gene031833.t1 MTR_2g034260 26.386 451 245 13 45 475 201 584 4.45e-32 129
MS.gene031833.t1 MTR_2g089100 24.336 452 291 10 35 474 30 442 1.49e-31 126
MS.gene031833.t1 MTR_4g097080 23.973 438 252 10 53 475 132 503 2.60e-30 123
MS.gene031833.t1 MTR_3g022005 51.546 97 46 1 382 478 33 128 3.20e-30 114
MS.gene031833.t1 MTR_2g097310 28.736 261 170 5 29 281 251 503 4.30e-30 124
MS.gene031833.t1 MTR_5g058860 25.455 440 275 8 40 474 105 496 4.71e-30 122
MS.gene031833.t1 MTR_3g072710 25.490 459 251 14 41 474 107 499 4.80e-30 122
MS.gene031833.t1 MTR_5g094450 31.111 225 146 3 38 254 146 369 3.44e-29 120
MS.gene031833.t1 MTR_5g094450 38.298 141 86 1 335 475 393 532 1.78e-25 109
MS.gene031833.t1 MTR_4g064200 30.667 225 143 4 51 269 281 498 1.62e-28 119
MS.gene031833.t1 MTR_4g064200 32.000 150 101 1 326 474 500 649 4.43e-16 81.3
MS.gene031833.t1 MTR_7g109580 25.773 485 277 14 7 475 137 554 1.01e-27 116
MS.gene031833.t1 MTR_3g027430 44.068 118 45 1 137 254 184 280 5.53e-26 108
MS.gene031833.t1 MTR_4g095500 23.622 508 306 12 3 474 6 467 6.24e-25 107
MS.gene031833.t1 MTR_5g015950 24.124 485 285 14 25 474 9 445 1.38e-24 106
MS.gene031833.t1 MTR_3g053270 24.124 485 285 14 25 474 9 445 1.38e-24 106
MS.gene031833.t1 MTR_2g097380 27.751 209 130 4 64 269 233 423 6.69e-24 105
MS.gene031833.t1 MTR_5g015490 31.193 218 140 4 48 256 108 324 7.68e-24 104
MS.gene031833.t1 MTR_4g133660 30.698 215 141 3 49 256 170 383 8.86e-24 104
MS.gene031833.t1 MTR_4g133660 34.228 149 97 1 326 474 395 542 1.61e-21 97.8
MS.gene031833.t1 MTR_1g086970 33.728 169 102 5 313 475 314 478 1.73e-21 97.4
MS.gene031833.t1 MTR_1g086970 25.403 248 164 8 45 272 97 343 1.66e-12 69.7
MS.gene031833.t1 MTR_4g076020 30.570 193 118 4 58 247 88 267 2.12e-21 96.7
MS.gene031833.t1 MTR_4g076020 39.597 149 84 4 329 475 285 429 1.16e-19 91.7
MS.gene031833.t1 MTR_2g099110 30.556 216 138 5 45 250 65 278 2.17e-21 97.1
MS.gene031833.t1 MTR_3g021320 34.641 153 72 2 154 306 1 125 7.01e-20 85.9
MS.gene031833.t1 MTR_4g077760 28.251 223 150 3 51 266 190 409 2.99e-19 90.9
MS.gene031833.t1 MTR_4g077760 30.055 183 115 6 301 475 378 555 2.73e-13 72.4
MS.gene031833.t1 MTR_7g057230 33.333 150 95 3 326 474 511 656 3.59e-19 90.9
MS.gene031833.t1 MTR_6g047750 27.933 358 184 13 130 475 367 662 1.04e-18 89.7
MS.gene031833.t1 MTR_0092s0100 33.529 170 105 3 25 194 352 513 5.05e-15 78.2
MS.gene031833.t1 MTR_0092s0100 33.529 170 105 3 25 194 349 510 5.36e-15 78.2
MS.gene031833.t1 MTR_8g093070 29.126 206 132 8 51 251 136 332 5.81e-13 71.2
MS.gene031833.t1 MTR_3g025340 45.902 61 33 0 112 172 9 69 6.80e-12 61.2
MS.gene031833.t1 MTR_8g020840 22.560 461 247 14 48 475 165 548 1.02e-11 67.4
MS.gene031833.t1 MTR_4g026485 31.351 185 113 4 55 233 270 446 1.35e-11 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031833.t1 AT1G50420 62.526 483 170 5 5 478 2 482 0.0 611
MS.gene031833.t1 AT1G66350 31.808 437 222 8 42 475 143 506 2.23e-67 224
MS.gene031833.t1 AT5G48150 30.445 427 232 5 55 475 123 490 1.80e-65 219
MS.gene031833.t1 AT5G48150 30.445 427 232 5 55 475 123 490 1.80e-65 219
MS.gene031833.t1 AT5G48150 30.445 427 232 5 55 475 123 490 1.80e-65 219
MS.gene031833.t1 AT5G48150 30.445 427 232 5 55 475 123 490 1.80e-65 219
MS.gene031833.t1 AT2G04890 32.701 422 223 5 54 475 53 413 8.74e-65 215
MS.gene031833.t1 AT5G17490 30.684 453 230 12 37 475 134 516 1.56e-59 204
MS.gene031833.t1 AT1G50600 29.322 457 251 9 30 475 131 526 8.12e-56 194
MS.gene031833.t1 AT1G21450 30.536 429 233 7 53 475 224 593 2.21e-55 194
MS.gene031833.t1 AT1G50600 29.386 456 250 9 31 475 207 601 2.71e-55 194
MS.gene031833.t1 AT1G50600 29.386 456 250 9 31 475 203 597 3.02e-55 194
MS.gene031833.t1 AT5G41920 29.561 433 229 11 51 477 40 402 9.62e-45 162
MS.gene031833.t1 AT1G07530 27.727 440 228 10 54 474 396 764 1.16e-43 164
MS.gene031833.t1 AT3G54220 41.706 211 121 1 39 247 278 488 1.88e-40 154
MS.gene031833.t1 AT3G54220 35.616 146 92 2 332 477 509 652 7.41e-20 93.2
MS.gene031833.t1 AT2G29060 27.189 434 246 10 51 474 281 654 3.95e-40 153
MS.gene031833.t1 AT2G29060 27.189 434 246 10 51 474 318 691 5.23e-40 153
MS.gene031833.t1 AT1G07520 25.508 443 262 7 43 474 143 528 7.10e-40 151
MS.gene031833.t1 AT1G07520 25.508 443 262 7 43 474 143 528 7.10e-40 151
MS.gene031833.t1 AT1G07520 25.508 443 262 7 43 474 307 692 3.12e-39 151
MS.gene031833.t1 AT1G07520 25.508 443 262 7 43 474 308 693 3.52e-39 151
MS.gene031833.t1 AT5G66770 28.670 436 232 12 51 475 217 584 1.67e-38 148
MS.gene031833.t1 AT2G29065 25.571 438 247 9 54 474 252 627 1.33e-37 145
MS.gene031833.t1 AT5G59450 25.576 434 255 7 51 475 224 598 4.72e-37 144
MS.gene031833.t1 AT2G37650 24.826 431 257 6 51 475 332 701 3.28e-36 142
MS.gene031833.t1 AT2G37650 24.826 431 257 6 51 475 344 713 3.42e-36 142
MS.gene031833.t1 AT3G46600 25.694 432 255 6 53 478 3 374 1.21e-35 136
MS.gene031833.t1 AT3G46600 25.694 432 255 6 53 478 179 550 8.29e-35 137
MS.gene031833.t1 AT3G46600 25.618 445 264 7 40 478 199 582 1.96e-34 136
MS.gene031833.t1 AT1G63100 32.636 239 156 4 46 280 269 506 3.20e-32 130
MS.gene031833.t1 AT1G63100 33.125 160 94 2 328 474 494 653 1.24e-20 95.5
MS.gene031833.t1 AT1G63100 32.636 239 156 4 46 280 244 481 3.96e-32 130
MS.gene031833.t1 AT1G63100 33.125 160 94 2 328 474 469 628 8.70e-21 95.9
MS.gene031833.t1 AT1G14920 33.921 227 130 5 42 258 160 376 8.52e-32 128
MS.gene031833.t1 AT1G14920 37.748 151 90 3 326 475 382 529 4.26e-27 114
MS.gene031833.t1 AT2G01570 33.921 227 130 5 42 258 212 428 2.42e-31 127
MS.gene031833.t1 AT2G01570 39.735 151 87 4 326 475 434 581 1.44e-27 116
MS.gene031833.t1 AT5G52510 26.147 436 247 13 53 475 267 640 2.65e-30 124
MS.gene031833.t1 AT3G03450 31.390 223 144 5 42 258 171 390 9.98e-30 122
MS.gene031833.t1 AT3G03450 38.854 157 94 2 320 475 390 545 9.28e-29 119
MS.gene031833.t1 AT1G55580 38.255 149 91 1 328 475 297 445 4.32e-28 116
MS.gene031833.t1 AT1G55580 27.451 255 144 8 50 264 40 293 2.01e-12 69.3
MS.gene031833.t1 AT4G37650 31.579 247 139 7 27 256 131 364 1.09e-23 104
MS.gene031833.t1 AT4G17230 34.000 150 98 1 326 475 377 525 6.53e-23 102
MS.gene031833.t1 AT4G17230 28.774 212 144 2 49 254 153 363 4.30e-22 99.8
MS.gene031833.t1 AT4G17230 34.000 150 98 1 326 475 377 525 6.53e-23 102
MS.gene031833.t1 AT4G17230 28.774 212 144 2 49 254 153 363 4.30e-22 99.8
MS.gene031833.t1 AT4G08250 29.963 267 155 10 1 253 68 316 5.62e-21 95.9
MS.gene031833.t1 AT3G50650 31.604 212 128 4 59 263 180 381 3.56e-17 84.7
MS.gene031833.t1 AT3G50650 28.859 149 99 3 334 475 394 542 3.87e-13 72.0
MS.gene031833.t1 AT3G49950 21.258 461 280 13 32 474 12 407 7.90e-15 76.6
MS.gene031833.t1 AT4G00150 26.667 195 134 5 286 477 368 556 5.20e-11 65.5

Find 106 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTCATTCGAGAGATTT+AGG 0.125804 4.3:-32108404 MS.gene031833:CDS
AATGAGCCTCTAAGTTATTT+TGG 0.189632 4.3:-32108258 MS.gene031833:CDS
TTTCATCATCCCAGGCTAAA+AGG 0.243724 4.3:+32108772 None:intergenic
CCGAATGTGCTTCGGTTTAC+TGG 0.250507 4.3:+32108684 None:intergenic
TGCAGCACTATTTGATTGTT+TGG 0.256288 4.3:-32108442 MS.gene031833:CDS
CAATCCTGTTGTCAGCAAAT+TGG 0.264169 4.3:-32108871 MS.gene031833:CDS
CATCATGGAGAAGAGCTTTA+AGG 0.269652 4.3:+32109481 None:intergenic
AGATTTGATTTAGCTGGATT+TGG 0.318965 4.3:-32108282 MS.gene031833:CDS
CAATATCAGCTTGGAGATCT+AGG 0.325494 4.3:-32108128 MS.gene031833:CDS
AATCTCTCGAATGAGTTTCT+TGG 0.330855 4.3:+32108408 None:intergenic
ATGCCACCTTCATCTGGCTT+TGG 0.334564 4.3:-32109461 MS.gene031833:CDS
AGCATTTGTTCTCACAAATC+AGG 0.349043 4.3:-32109114 MS.gene031833:CDS
TCCATGATGCCACCTTCATC+TGG 0.349847 4.3:-32109467 MS.gene031833:CDS
AACTTACCAAAATAACTTAG+AGG 0.353618 4.3:+32108252 None:intergenic
TTGAGTGCTTTGTGGATTCC+AGG 0.357455 4.3:+32109215 None:intergenic
AAACTCATTCGAGAGATTTA+GGG 0.358753 4.3:-32108403 MS.gene031833:CDS
TGCTGACAACAGGATTGAAT+TGG 0.362322 4.3:+32108877 None:intergenic
CAATTTGCTTGAGAAAGATT+TGG 0.366215 4.3:-32108661 MS.gene031833:CDS
ATCTCCACACCCTTGGCTTA+AGG 0.366272 4.3:-32109438 MS.gene031833:CDS
TATAGAATGAAGGAGGAAAA+TGG 0.386592 4.3:-32108189 MS.gene031833:CDS
TGAACTTGAGTCAGGACTTA+TGG 0.391992 4.3:+32108626 None:intergenic
ATCTCTCGAATGAGTTTCTT+GGG 0.394420 4.3:+32108409 None:intergenic
CTCAAATGTGGAGGGCCATC+AGG 0.400333 4.3:+32108987 None:intergenic
GAGAAACTTCGTGTTAAAAC+CGG 0.400451 4.3:-32108834 MS.gene031833:CDS
CAGGATGAGCACTCAAAACT+TGG 0.403034 4.3:+32109006 None:intergenic
TTACCGAAGCACTTGCTGAT+AGG 0.405714 4.3:-32109253 MS.gene031833:CDS
CACGAAGTTTCTCAAAATCA+AGG 0.414533 4.3:+32108844 None:intergenic
GCTTTGGATCTCCACACCCT+TGG 0.415132 4.3:-32109445 MS.gene031833:CDS
GAGTAGAGTTGAGTGCTTTG+TGG 0.416744 4.3:+32109207 None:intergenic
TTTATGAGATTTGATTTAGC+TGG 0.436460 4.3:-32108288 MS.gene031833:CDS
AGGCTAAAAGGGTATGCAAT+TGG 0.439239 4.3:+32108784 None:intergenic
AGGGTGGAGAAACTTCATTT+TGG 0.439547 4.3:-32108384 MS.gene031833:CDS
AAGAGGTTCATGCAGAGTTA+TGG 0.445441 4.3:-32108222 MS.gene031833:CDS
CGCTGCATAGTATCGCCGTC+AGG 0.446613 4.3:+32109290 None:intergenic
GGAACTGAAATCTGAGGAAA+GGG 0.447352 4.3:-32109417 MS.gene031833:CDS
CCAGTAAACCGAAGCACATT+CGG 0.448222 4.3:-32108684 MS.gene031833:CDS
CTTCATGGTTTCATCATCCC+AGG 0.449792 4.3:+32108764 None:intergenic
GAACAAGACTCTAATCATAA+TGG 0.450476 4.3:-32108507 MS.gene031833:CDS
TGCTAATATGCTGGCAAGAT+AGG 0.451606 4.3:-32108161 MS.gene031833:CDS
TCTGTTACAACCATCACCTT+TGG 0.451615 4.3:+32108528 None:intergenic
AAAGGAAAGGCATGAAAAGC+TGG 0.456379 4.3:-32108319 MS.gene031833:CDS
GCAAATTGCCGAATGTGCTT+CGG 0.471544 4.3:+32108676 None:intergenic
TTCATCATCCCAGGCTAAAA+GGG 0.472082 4.3:+32108773 None:intergenic
CAACTCTACTCGAGTGTCAA+TGG 0.491983 4.3:-32109195 MS.gene031833:CDS
TTGCACAAGTGAGCAACAAA+TGG 0.493036 4.3:+32109381 None:intergenic
GCCGTCAGGGGAAGCGAGCA+TGG 0.502474 4.3:+32109304 None:intergenic
ATGTGCTTCGGTTTACTGGA+TGG 0.504499 4.3:+32108688 None:intergenic
ACAAATGCTACTTTCATGAA+AGG 0.509715 4.3:+32109128 None:intergenic
AGGATACTTAAAGCATGGCC+TGG 0.511478 4.3:-32109233 MS.gene031833:CDS
GGTGATGATGAACTTGAGTC+AGG 0.512355 4.3:+32108618 None:intergenic
TTGTAAGTGAATTCCATTCG+AGG 0.515092 4.3:+32108716 None:intergenic
CAAATGCTACTTTCATGAAA+GGG 0.516070 4.3:+32109129 None:intergenic
TCAGGCTATCATTGAAGCAA+TGG 0.520201 4.3:-32109096 MS.gene031833:CDS
GCCAGATGAAGGTGGCATCA+TGG 0.521665 4.3:+32109466 None:intergenic
TTTGAAGTGTTCAAAGAAGC+CGG 0.525201 4.3:+32108597 None:intergenic
CAAGAACATTATTGCATGTG+AGG 0.529680 4.3:-32108352 MS.gene031833:CDS
TCGAATGGAATTCACTTACA+AGG 0.530422 4.3:-32108714 MS.gene031833:CDS
AGAAACTTCGTGTTAAAACC+GGG 0.531168 4.3:-32108833 MS.gene031833:CDS
GGCATGAAAAGCTGGATAAG+TGG 0.531394 4.3:-32108311 MS.gene031833:CDS
ATGTGAGGGATTAGAAAGAA+AGG 0.534942 4.3:-32108337 MS.gene031833:CDS
TCCATGCTCGCTTCCCCTGA+CGG 0.536287 4.3:-32109305 MS.gene031833:CDS
TATCCTATCAGCAAGTGCTT+CGG 0.537933 4.3:+32109250 None:intergenic
ATTTGATTGTTTGGAATCAA+CGG 0.538609 4.3:-32108433 MS.gene031833:CDS
ACTAATTGCTAGTGCCTCCC+CGG 0.543403 4.3:+32108815 None:intergenic
AGGAACTGAAATCTGAGGAA+AGG 0.544931 4.3:-32109418 MS.gene031833:CDS
GATTTCAGTTCCTTAAGCCA+AGG 0.546043 4.3:+32109428 None:intergenic
TTGTGAGGCATATAGAATGA+AGG 0.546355 4.3:-32108199 MS.gene031833:CDS
GTACAGCTATGTTGCAAGAA+GGG 0.546911 4.3:-32109526 MS.gene031833:CDS
AGAAATGAAATCCACTGAGC+AGG 0.550035 4.3:+32109029 None:intergenic
CCAAGCTGATATTGAAAACA+AGG 0.551538 4.3:+32108137 None:intergenic
AGTTCCTTAAGCCAAGGGTG+TGG 0.551766 4.3:+32109434 None:intergenic
ATGGCTGTGTGCTAATATGC+TGG 0.553571 4.3:-32108170 MS.gene031833:CDS
CAATCAGTCTATGAGCTACC+TGG 0.553683 4.3:+32108928 None:intergenic
CCTCCACATTTGAGAATCAC+CGG 0.556070 4.3:-32108978 MS.gene031833:CDS
GAAGAAAGAGGTTCTCGACC+AGG 0.556759 4.3:-32108946 MS.gene031833:CDS
ATTTCAGTTCCTTAAGCCAA+GGG 0.557071 4.3:+32109429 None:intergenic
TGACTCAAGTTCATCATCAC+CGG 0.560351 4.3:-32108616 MS.gene031833:CDS
CTCTTTCTTCTGATGAACGC+CGG 0.562200 4.3:+32108959 None:intergenic
AGTGTAGAGCTTCTAGTAGC+CGG 0.564547 4.3:+32108469 None:intergenic
CTCATTCGAGAGATTTAGGG+TGG 0.565611 4.3:-32108400 MS.gene031833:CDS
AAGAACATTATTGCATGTGA+GGG 0.567712 4.3:-32108351 MS.gene031833:CDS
GGTACAGCTATGTTGCAAGA+AGG 0.572547 4.3:-32109527 MS.gene031833:CDS
GGAGATCCAAAGCCAGATGA+AGG 0.572774 4.3:+32109455 None:intergenic
GAGTTTATCACCAAAGGTGA+TGG 0.577759 4.3:-32108538 MS.gene031833:CDS
ACAACAGGATTGAATTGGAA+TGG 0.580239 4.3:+32108882 None:intergenic
CATGCAGAGTTATGGTTGTG+AGG 0.584714 4.3:-32108214 MS.gene031833:CDS
GCTGCATAGTATCGCCGTCA+GGG 0.585579 4.3:+32109291 None:intergenic
CGGCGTTCATCAGAAGAAAG+AGG 0.593327 4.3:-32108958 MS.gene031833:CDS
TTGGTAAGTTGCAAGCAAAG+AGG 0.603635 4.3:-32108239 MS.gene031833:CDS
CTGATAGGATACTTAAAGCA+TGG 0.603739 4.3:-32109238 MS.gene031833:CDS
AGACATTTCTCAATGCATTG+TGG 0.605127 4.3:-32108560 MS.gene031833:CDS
GCTTAAGGAACTGAAATCTG+AGG 0.605648 4.3:-32109423 MS.gene031833:CDS
ATTGTGGAGTTTATCACCAA+AGG 0.618172 4.3:-32108544 MS.gene031833:CDS
CGGTGATTCTCAAATGTGGA+GGG 0.619559 4.3:+32108979 None:intergenic
TGAGGCATATAGAATGAAGG+AGG 0.622680 4.3:-32108196 MS.gene031833:CDS
AGGGATTAGAAAGAAAGGAA+AGG 0.622681 4.3:-32108332 MS.gene031833:CDS
ACGCCGGTGATTCTCAAATG+TGG 0.623446 4.3:+32108975 None:intergenic
CTGCATAGTATCGCCGTCAG+GGG 0.626726 4.3:+32109292 None:intergenic
CCGGTGATTCTCAAATGTGG+AGG 0.626889 4.3:+32108978 None:intergenic
GCTATCATTGAAGCAATGGA+AGG 0.639218 4.3:-32109092 MS.gene031833:CDS
ATGCTGCTGAACCTGCTCAG+TGG 0.654432 4.3:-32109040 MS.gene031833:CDS
GATCCAAAGCCAGATGAAGG+TGG 0.658186 4.3:+32109458 None:intergenic
GAAACTTCGTGTTAAAACCG+GGG 0.688408 4.3:-32108832 MS.gene031833:CDS
GAACTGAAATCTGAGGAAAG+GGG 0.705778 4.3:-32109416 MS.gene031833:CDS
ACTTCGTGTTAAAACCGGGG+AGG 0.735326 4.3:-32108829 MS.gene031833:CDS
CTGACGGCGATACTATGCAG+CGG 0.754112 4.3:-32109289 MS.gene031833:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACTTACCAAAATAACTTAG+AGG + chr4.3:32109409-32109428 None:intergenic 25.0%
!! ATTTGATTGTTTGGAATCAA+CGG - chr4.3:32109225-32109244 MS.gene031833:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG - chr4.3:32109370-32109389 MS.gene031833:CDS 25.0%
AAACTCATTCGAGAGATTTA+GGG - chr4.3:32109255-32109274 MS.gene031833:CDS 30.0%
AAGAACATTATTGCATGTGA+GGG - chr4.3:32109307-32109326 MS.gene031833:CDS 30.0%
GAACAAGACTCTAATCATAA+TGG - chr4.3:32109151-32109170 MS.gene031833:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG - chr4.3:32109469-32109488 MS.gene031833:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG - chr4.3:32109400-32109419 MS.gene031833:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG - chr4.3:32109376-32109395 MS.gene031833:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG - chr4.3:32108997-32109016 MS.gene031833:CDS 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG - chr4.3:32108929-32108948 MS.gene031833:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG - chr4.3:32109165-32109184 MS.gene031833:CDS 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG + chr4.3:32108929-32108948 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG + chr4.3:32108533-32108552 None:intergenic 30.0%
!! CAAATGCTACTTTCATGAAA+GGG + chr4.3:32108532-32108551 None:intergenic 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG + chr4.3:32108912-32108931 None:intergenic 30.0%
AATCTCTCGAATGAGTTTCT+TGG + chr4.3:32109253-32109272 None:intergenic 35.0%
ACAACAGGATTGAATTGGAA+TGG + chr4.3:32108779-32108798 None:intergenic 35.0%
AGAAACTTCGTGTTAAAACC+GGG - chr4.3:32108825-32108844 MS.gene031833:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG - chr4.3:32109098-32109117 MS.gene031833:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG - chr4.3:32108544-32108563 MS.gene031833:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG - chr4.3:32109326-32109345 MS.gene031833:CDS 35.0%
ATCTCTCGAATGAGTTTCTT+GGG + chr4.3:32109252-32109271 None:intergenic 35.0%
ATGTGAGGGATTAGAAAGAA+AGG - chr4.3:32109321-32109340 MS.gene031833:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG - chr4.3:32109114-32109133 MS.gene031833:CDS 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG + chr4.3:32108232-32108251 None:intergenic 35.0%
CAAGAACATTATTGCATGTG+AGG - chr4.3:32109306-32109325 MS.gene031833:CDS 35.0%
CACGAAGTTTCTCAAAATCA+AGG + chr4.3:32108817-32108836 None:intergenic 35.0%
CCAAGCTGATATTGAAAACA+AGG + chr4.3:32109524-32109543 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG - chr4.3:32108420-32108439 MS.gene031833:CDS 35.0%
GAAACTCATTCGAGAGATTT+AGG - chr4.3:32109254-32109273 MS.gene031833:CDS 35.0%
GAGAAACTTCGTGTTAAAAC+CGG - chr4.3:32108824-32108843 MS.gene031833:CDS 35.0%
TCGAATGGAATTCACTTACA+AGG - chr4.3:32108944-32108963 MS.gene031833:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG - chr4.3:32109216-32109235 MS.gene031833:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG + chr4.3:32108945-32108964 None:intergenic 35.0%
TTGTGAGGCATATAGAATGA+AGG - chr4.3:32109459-32109478 MS.gene031833:CDS 35.0%
! TTTGAAGTGTTCAAAGAAGC+CGG + chr4.3:32109064-32109083 None:intergenic 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG - chr4.3:32109521-32109540 MS.gene031833:CDS 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG + chr4.3:32108794-32108813 None:intergenic 35.0%
AAGAGGTTCATGCAGAGTTA+TGG - chr4.3:32109436-32109455 MS.gene031833:CDS 40.0%
AGAAATGAAATCCACTGAGC+AGG + chr4.3:32108632-32108651 None:intergenic 40.0%
AGGAACTGAAATCTGAGGAA+AGG - chr4.3:32108240-32108259 MS.gene031833:CDS 40.0%
CAATATCAGCTTGGAGATCT+AGG - chr4.3:32109530-32109549 MS.gene031833:CDS 40.0%
CAATCCTGTTGTCAGCAAAT+TGG - chr4.3:32108787-32108806 MS.gene031833:CDS 40.0%
CATCATGGAGAAGAGCTTTA+AGG + chr4.3:32108180-32108199 None:intergenic 40.0%
GAAACTTCGTGTTAAAACCG+GGG - chr4.3:32108826-32108845 MS.gene031833:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG - chr4.3:32108242-32108261 MS.gene031833:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG - chr4.3:32109120-32109139 MS.gene031833:CDS 40.0%
GATTTCAGTTCCTTAAGCCA+AGG + chr4.3:32108233-32108252 None:intergenic 40.0%
GCTATCATTGAAGCAATGGA+AGG - chr4.3:32108566-32108585 MS.gene031833:CDS 40.0%
GCTTAAGGAACTGAAATCTG+AGG - chr4.3:32108235-32108254 MS.gene031833:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG - chr4.3:32108241-32108260 MS.gene031833:CDS 40.0%
GTACAGCTATGTTGCAAGAA+GGG - chr4.3:32108132-32108151 MS.gene031833:CDS 40.0%
TATCCTATCAGCAAGTGCTT+CGG + chr4.3:32108411-32108430 None:intergenic 40.0%
TCAGGCTATCATTGAAGCAA+TGG - chr4.3:32108562-32108581 MS.gene031833:CDS 40.0%
TCTGTTACAACCATCACCTT+TGG + chr4.3:32109133-32109152 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG + chr4.3:32109035-32109054 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG - chr4.3:32109042-32109061 MS.gene031833:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG - chr4.3:32109462-32109481 MS.gene031833:CDS 40.0%
TGCTAATATGCTGGCAAGAT+AGG - chr4.3:32109497-32109516 MS.gene031833:CDS 40.0%
TGCTGACAACAGGATTGAAT+TGG + chr4.3:32108784-32108803 None:intergenic 40.0%
TTCATCATCCCAGGCTAAAA+GGG + chr4.3:32108888-32108907 None:intergenic 40.0%
TTGCACAAGTGAGCAACAAA+TGG + chr4.3:32108280-32108299 None:intergenic 40.0%
TTGGTAAGTTGCAAGCAAAG+AGG - chr4.3:32109419-32109438 MS.gene031833:CDS 40.0%
TTTCATCATCCCAGGCTAAA+AGG + chr4.3:32108889-32108908 None:intergenic 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG - chr4.3:32109339-32109358 MS.gene031833:CDS 40.0%
! AATTGCATACCCTTTTAGCC+TGG - chr4.3:32108876-32108895 MS.gene031833:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG + chr4.3:32108877-32108896 None:intergenic 40.0%
! AGGGTGGAGAAACTTCATTT+TGG - chr4.3:32109274-32109293 MS.gene031833:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG - chr4.3:32108877-32108896 MS.gene031833:CDS 40.0%
!! GGAGAAACTTCATTTTGGTG+AGG - chr4.3:32109279-32109298 MS.gene031833:CDS 40.0%
AGGATACTTAAAGCATGGCC+TGG - chr4.3:32108425-32108444 MS.gene031833:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG + chr4.3:32109192-32109211 None:intergenic 45.0%
ATGGCTGTGTGCTAATATGC+TGG - chr4.3:32109488-32109507 MS.gene031833:CDS 45.0%
ATGTGCTTCGGTTTACTGGA+TGG + chr4.3:32108973-32108992 None:intergenic 45.0%
CAACTCTACTCGAGTGTCAA+TGG - chr4.3:32108463-32108482 MS.gene031833:CDS 45.0%
CAATCAGTCTATGAGCTACC+TGG + chr4.3:32108733-32108752 None:intergenic 45.0%
CAGGATGAGCACTCAAAACT+TGG + chr4.3:32108655-32108674 None:intergenic 45.0%
CATGCAGAGTTATGGTTGTG+AGG - chr4.3:32109444-32109463 MS.gene031833:CDS 45.0%
CCAGTAAACCGAAGCACATT+CGG - chr4.3:32108974-32108993 MS.gene031833:CDS 45.0%
CCTCCACATTTGAGAATCAC+CGG - chr4.3:32108680-32108699 MS.gene031833:CDS 45.0%
CGGTGATTCTCAAATGTGGA+GGG + chr4.3:32108682-32108701 None:intergenic 45.0%
CTCATTCGAGAGATTTAGGG+TGG - chr4.3:32109258-32109277 MS.gene031833:CDS 45.0%
CTCTTTCTTCTGATGAACGC+CGG + chr4.3:32108702-32108721 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG + chr4.3:32108897-32108916 None:intergenic 45.0%
GAGTAGAGTTGAGTGCTTTG+TGG + chr4.3:32108454-32108473 None:intergenic 45.0%
GCAAATTGCCGAATGTGCTT+CGG + chr4.3:32108985-32109004 None:intergenic 45.0%
GGCATGAAAAGCTGGATAAG+TGG - chr4.3:32109347-32109366 MS.gene031833:CDS 45.0%
GGTACAGCTATGTTGCAAGA+AGG - chr4.3:32108131-32108150 MS.gene031833:CDS 45.0%
TTGAGTGCTTTGTGGATTCC+AGG + chr4.3:32108446-32108465 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG - chr4.3:32109170-32109189 MS.gene031833:CDS 45.0%
! GGTGATGATGAACTTGAGTC+AGG + chr4.3:32109043-32109062 None:intergenic 45.0%
! TTACCGAAGCACTTGCTGAT+AGG - chr4.3:32108405-32108424 MS.gene031833:CDS 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG - chr4.3:32108656-32108675 MS.gene031833:CDS 45.0%
ACGCCGGTGATTCTCAAATG+TGG + chr4.3:32108686-32108705 None:intergenic 50.0%
ACTAATTGCTAGTGCCTCCC+CGG + chr4.3:32108846-32108865 None:intergenic 50.0%
ACTTCGTGTTAAAACCGGGG+AGG - chr4.3:32108829-32108848 MS.gene031833:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG + chr4.3:32108227-32108246 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG - chr4.3:32108220-32108239 MS.gene031833:CDS 50.0%
CCGAATGTGCTTCGGTTTAC+TGG + chr4.3:32108977-32108996 None:intergenic 50.0%
CCGGTGATTCTCAAATGTGG+AGG + chr4.3:32108683-32108702 None:intergenic 50.0%
CGGCGTTCATCAGAAGAAAG+AGG - chr4.3:32108700-32108719 MS.gene031833:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG - chr4.3:32108712-32108731 MS.gene031833:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG + chr4.3:32108203-32108222 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG + chr4.3:32108206-32108225 None:intergenic 50.0%
TCCATGATGCCACCTTCATC+TGG - chr4.3:32108191-32108210 MS.gene031833:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG - chr4.3:32108197-32108216 MS.gene031833:CDS 50.0%
ATGCTGCTGAACCTGCTCAG+TGG - chr4.3:32108618-32108637 MS.gene031833:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG + chr4.3:32108674-32108693 None:intergenic 55.0%
CTGACGGCGATACTATGCAG+CGG - chr4.3:32108369-32108388 MS.gene031833:CDS 55.0%
CTGCATAGTATCGCCGTCAG+GGG + chr4.3:32108369-32108388 None:intergenic 55.0%
GCCAGATGAAGGTGGCATCA+TGG + chr4.3:32108195-32108214 None:intergenic 55.0%
GCTGCATAGTATCGCCGTCA+GGG + chr4.3:32108370-32108389 None:intergenic 55.0%
! GCTTTGGATCTCCACACCCT+TGG - chr4.3:32108213-32108232 MS.gene031833:CDS 55.0%
CGCTGCATAGTATCGCCGTC+AGG + chr4.3:32108371-32108390 None:intergenic 60.0%
TCCATGCTCGCTTCCCCTGA+CGG - chr4.3:32108353-32108372 MS.gene031833:CDS 60.0%
GCCGTCAGGGGAAGCGAGCA+TGG + chr4.3:32108357-32108376 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 32108122 32109558 32108122 ID=MS.gene031833
chr4.3 mRNA 32108122 32109558 32108122 ID=MS.gene031833.t1;Parent=MS.gene031833
chr4.3 exon 32108122 32109558 32108122 ID=MS.gene031833.t1.exon1;Parent=MS.gene031833.t1
chr4.3 CDS 32108122 32109558 32108122 ID=cds.MS.gene031833.t1;Parent=MS.gene031833.t1
Gene Sequence

>MS.gene031833

ATGTTACATGGTACAGCTATGTTGCAAGAAGGGTCATCTTCTGTTACTTCTTCAGCCTTAAAGCTCTTCTCCATGATGCCACCTTCATCTGGCTTTGGATCTCCACACCCTTGGCTTAAGGAACTGAAATCTGAGGAAAGGGGTTTGTACTTGATCCATTTGTTGCTCACTTGTGCAAATCATGTAGCTGCTTGTAATCTTGAGCATGCAAATGCAACACTTGAGCAAATTTCCATGCTCGCTTCCCCTGACGGCGATACTATGCAGCGGATTGCAGCGTATTTTACCGAAGCACTTGCTGATAGGATACTTAAAGCATGGCCTGGAATCCACAAAGCACTCAACTCTACTCGAGTGTCAATGGTTTCAGAAAAGATTCTTGTGCAGAAGCTCTTTTTTGAGCTTTTCCCTTTCATGAAAGTAGCATTTGTTCTCACAAATCAGGCTATCATTGAAGCAATGGAAGGCGAGAGAATGATTCACATAATTGATCTCAATGCTGCTGAACCTGCTCAGTGGATTTCATTTCTCCAAGTTTTGAGTGCTCATCCTGATGGCCCTCCACATTTGAGAATCACCGGCGTTCATCAGAAGAAAGAGGTTCTCGACCAGGTAGCTCATAGACTGATTGCAGAAGCTGAAAAATTAGATATACCATTCCAATTCAATCCTGTTGTCAGCAAATTGGAAAACCTTGATTTTGAGAAACTTCGTGTTAAAACCGGGGAGGCACTAGCAATTAGTTCAATTCTCCAATTGCATACCCTTTTAGCCTGGGATGATGAAACCATGAAGAAAAAATCTCCTCTTTTGTTAAAAACCTCGAATGGAATTCACTTACAAGGATTCCATCCAGTAAACCGAAGCACATTCGGCAATTTGCTTGAGAAAGATTTGGTTAGCAGCTATACCATAAGTCCTGACTCAAGTTCATCATCACCGGCTTCTTTGAACACTTCAAATTCAATGAACACAGAGACATTTCTCAATGCATTGTGGAGTTTATCACCAAAGGTGATGGTTGTAACAGAACAAGACTCTAATCATAATGGCTCACATTTTACGGACCGGCTACTAGAAGCTCTACACTCTTATGCAGCACTATTTGATTGTTTGGAATCAACGGTCCCAAGAAACTCATTCGAGAGATTTAGGGTGGAGAAACTTCATTTTGGTGAGGAAATCAAGAACATTATTGCATGTGAGGGATTAGAAAGAAAGGAAAGGCATGAAAAGCTGGATAAGTGGTTTATGAGATTTGATTTAGCTGGATTTGGTAATGAGCCTCTAAGTTATTTTGGTAAGTTGCAAGCAAAGAGGTTCATGCAGAGTTATGGTTGTGAGGCATATAGAATGAAGGAGGAAAATGGCTGTGTGCTAATATGCTGGCAAGATAGGTCCTTGTTTTCAATATCAGCTTGGAGATCTAGGAAGTAA
Protein sequence

>MS.gene031833.t1

MLHGTAMLQEGSSSVTSSALKLFSMMPPSSGFGSPHPWLKELKSEERGLYLIHLLLTCANHVAACNLEHANATLEQISMLASPDGDTMQRIAAYFTEALADRILKAWPGIHKALNSTRVSMVSEKILVQKLFFELFPFMKVAFVLTNQAIIEAMEGERMIHIIDLNAAEPAQWISFLQVLSAHPDGPPHLRITGVHQKKEVLDQVAHRLIAEAEKLDIPFQFNPVVSKLENLDFEKLRVKTGEALAISSILQLHTLLAWDDETMKKKSPLLLKTSNGIHLQGFHPVNRSTFGNLLEKDLVSSYTISPDSSSSSPASLNTSNSMNTETFLNALWSLSPKVMVVTEQDSNHNGSHFTDRLLEALHSYAALFDCLESTVPRNSFERFRVEKLHFGEEIKNIIACEGLERKERHEKLDKWFMRFDLAGFGNEPLSYFGKLQAKRFMQSYGCEAYRMKEENGCVLICWQDRSLFSISAWRSRK