AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031838

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031838.t1 MTR_4g076020 88.997 309 20 2 1 309 17 311 1.18e-179 503
MS.gene031838.t1 MTR_7g074650 60.504 238 90 2 72 309 441 674 1.86e-95 300
MS.gene031838.t1 MTR_1g069725 51.417 247 112 2 63 308 103 342 3.64e-82 256
MS.gene031838.t1 MTR_7g027190 35.052 291 170 5 39 313 270 557 3.54e-48 171
MS.gene031838.t1 MTR_4g102790 36.719 256 155 4 63 312 286 540 1.22e-44 161
MS.gene031838.t1 MTR_3g065980 33.591 259 155 4 60 312 163 410 1.09e-38 143
MS.gene031838.t1 MTR_4g064150 29.927 274 180 3 51 312 343 616 3.97e-36 137
MS.gene031838.t1 MTR_5g058860 35.955 267 150 7 54 301 104 368 8.07e-34 129
MS.gene031838.t1 MTR_3g072710 36.559 279 152 9 53 308 104 380 8.75e-34 129
MS.gene031838.t1 MTR_4g104020 30.686 277 178 6 50 313 130 405 2.67e-33 128
MS.gene031838.t1 MTR_2g097410 28.159 277 173 4 54 312 356 624 4.20e-33 129
MS.gene031838.t1 MTR_1g029420 35.271 258 149 7 69 313 225 477 4.68e-33 128
MS.gene031838.t1 MTR_5g009080 32.524 206 136 2 60 265 47 249 1.32e-32 126
MS.gene031838.t1 MTR_4g064120 26.460 291 202 3 34 312 219 509 4.37e-32 125
MS.gene031838.t1 MTR_4g064180 28.231 294 193 6 34 312 219 509 1.91e-31 124
MS.gene031838.t1 MTR_3g089055 34.109 258 152 7 69 313 198 450 5.15e-31 122
MS.gene031838.t1 MTR_3g089055 35.039 254 146 8 69 308 203 451 6.13e-31 122
MS.gene031838.t1 MTR_5g094450 33.061 245 150 5 69 300 161 404 5.45e-30 119
MS.gene031838.t1 MTR_4g076140 33.810 210 128 3 60 265 39 241 6.99e-30 118
MS.gene031838.t1 MTR_4g077760 34.000 250 143 7 66 302 190 430 3.53e-29 117
MS.gene031838.t1 MTR_2g082090 30.104 289 181 7 24 300 172 451 1.03e-28 116
MS.gene031838.t1 MTR_8g442410 34.221 263 161 4 63 313 149 411 1.27e-28 115
MS.gene031838.t1 MTR_4g064200 29.434 265 166 6 61 312 276 532 2.27e-28 115
MS.gene031838.t1 MTR_5g097480 33.197 244 147 5 69 300 177 416 2.38e-28 114
MS.gene031838.t1 MTR_4g097080 30.081 246 153 7 68 300 132 371 3.34e-28 114
MS.gene031838.t1 MTR_2g099110 30.864 243 151 7 69 300 74 310 3.72e-28 113
MS.gene031838.t1 MTR_1g096030 33.333 255 145 8 59 300 70 312 5.62e-28 113
MS.gene031838.t1 MTR_8g093070 32.812 256 151 7 53 301 125 366 1.33e-27 112
MS.gene031838.t1 MTR_3g056110 29.961 257 164 5 69 313 175 427 2.44e-27 112
MS.gene031838.t1 MTR_5g015490 28.958 259 161 7 60 300 105 358 2.61e-27 111
MS.gene031838.t1 MTR_4g064160 27.737 274 182 5 51 312 298 567 8.27e-27 110
MS.gene031838.t1 MTR_2g097467 27.124 306 192 7 27 312 243 537 1.14e-26 110
MS.gene031838.t1 MTR_2g097310 30.660 212 137 4 64 273 267 470 1.29e-26 110
MS.gene031838.t1 MTR_7g062120 29.600 250 146 7 64 294 367 605 1.65e-26 110
MS.gene031838.t1 MTR_2g097350 32.195 205 133 3 64 266 269 469 5.65e-26 108
MS.gene031838.t1 MTR_2g097473 28.358 268 165 6 61 312 281 537 8.58e-26 107
MS.gene031838.t1 MTR_2g097463 28.358 268 165 6 61 312 282 538 1.45e-25 107
MS.gene031838.t1 MTR_3g022830 31.122 196 132 2 70 265 26 218 1.72e-25 105
MS.gene031838.t1 MTR_4g133660 31.200 250 155 6 64 300 170 415 4.16e-24 102
MS.gene031838.t1 MTR_2g097390 29.902 204 137 3 64 265 270 469 6.57e-24 102
MS.gene031838.t1 MTR_2g034280 29.213 267 177 3 59 313 193 459 2.24e-23 100
MS.gene031838.t1 MTR_2g034260 29.670 273 170 6 59 313 200 468 2.78e-23 100
MS.gene031838.t1 MTR_0092s0100 30.932 236 150 6 70 299 373 601 2.20e-22 97.8
MS.gene031838.t1 MTR_0092s0100 30.932 236 150 6 70 299 376 604 2.36e-22 97.8
MS.gene031838.t1 MTR_2g089100 24.460 278 175 5 69 312 52 328 5.97e-22 95.9
MS.gene031838.t1 MTR_2g026250 28.458 253 158 7 64 300 223 468 1.25e-21 95.5
MS.gene031838.t1 MTR_4g026485 29.339 242 149 6 70 300 270 500 1.90e-21 95.1
MS.gene031838.t1 MTR_2g097380 28.378 222 135 5 54 273 209 408 1.08e-20 92.8
MS.gene031838.t1 MTR_2g034250 27.473 273 176 6 59 313 200 468 1.09e-20 92.8
MS.gene031838.t1 MTR_7g109580 25.660 265 182 4 59 313 179 438 7.36e-19 87.4
MS.gene031838.t1 MTR_1g086970 22.388 268 194 6 59 313 96 362 4.82e-13 69.7
MS.gene031838.t1 MTR_8g077940 31.176 170 109 5 70 235 183 348 8.06e-13 69.3
MS.gene031838.t1 MTR_1g106590 30.000 120 82 1 63 182 124 241 1.25e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031838.t1 AT5G41920 69.231 260 77 2 53 309 27 286 1.40e-124 362
MS.gene031838.t1 AT3G54220 58.779 262 105 2 49 309 273 532 1.55e-97 301
MS.gene031838.t1 AT1G63100 35.827 254 157 4 64 312 247 499 1.31e-39 147
MS.gene031838.t1 AT1G63100 35.827 254 157 4 64 312 272 524 1.99e-39 147
MS.gene031838.t1 AT3G03450 34.615 260 158 5 60 312 174 428 1.44e-37 140
MS.gene031838.t1 AT1G66350 33.984 256 157 4 60 312 146 392 9.59e-35 132
MS.gene031838.t1 AT1G50420 35.437 206 132 1 60 265 48 252 1.58e-33 128
MS.gene031838.t1 AT5G17490 32.692 260 157 6 60 312 151 399 1.66e-32 126
MS.gene031838.t1 AT2G01570 30.268 261 165 5 60 312 215 466 4.25e-30 120
MS.gene031838.t1 AT1G14920 31.679 262 160 5 60 312 163 414 2.57e-29 117
MS.gene031838.t1 AT4G37650 29.572 257 156 7 69 305 146 397 2.73e-28 114
MS.gene031838.t1 AT1G07520 27.306 271 177 5 59 312 144 411 2.79e-28 114
MS.gene031838.t1 AT1G07520 27.306 271 177 5 59 312 144 411 2.79e-28 114
MS.gene031838.t1 AT1G07520 27.306 271 177 5 59 312 309 576 3.02e-28 115
MS.gene031838.t1 AT1G07520 27.306 271 177 5 59 312 308 575 3.14e-28 115
MS.gene031838.t1 AT1G07530 29.615 260 166 6 66 312 393 648 4.14e-28 114
MS.gene031838.t1 AT2G04890 33.333 243 149 6 64 300 50 285 6.67e-28 112
MS.gene031838.t1 AT5G48150 31.687 243 151 5 70 300 123 362 1.18e-27 112
MS.gene031838.t1 AT5G48150 31.687 243 151 5 70 300 123 362 1.18e-27 112
MS.gene031838.t1 AT5G48150 31.687 243 151 5 70 300 123 362 1.18e-27 112
MS.gene031838.t1 AT5G48150 31.687 243 151 5 70 300 123 362 1.18e-27 112
MS.gene031838.t1 AT5G66770 33.852 257 151 8 66 312 217 464 1.93e-27 112
MS.gene031838.t1 AT5G59450 27.636 275 180 5 52 312 210 479 2.13e-27 112
MS.gene031838.t1 AT1G50600 33.198 247 149 5 66 300 156 398 3.86e-27 111
MS.gene031838.t1 AT1G21450 31.008 258 160 6 69 313 225 477 4.37e-27 111
MS.gene031838.t1 AT1G50600 33.198 247 149 5 66 300 231 473 6.31e-27 111
MS.gene031838.t1 AT1G50600 33.198 247 149 5 66 300 227 469 6.49e-27 110
MS.gene031838.t1 AT3G50650 34.322 236 135 6 87 312 193 418 7.00e-27 110
MS.gene031838.t1 AT2G29060 33.679 193 115 4 66 249 281 469 1.02e-25 107
MS.gene031838.t1 AT2G29060 33.679 193 115 4 66 249 318 506 1.23e-25 107
MS.gene031838.t1 AT2G37650 28.352 261 169 6 64 312 330 584 1.96e-25 107
MS.gene031838.t1 AT2G37650 28.352 261 169 6 64 312 342 596 2.16e-25 107
MS.gene031838.t1 AT4G17230 32.061 262 162 5 64 313 153 410 3.58e-25 105
MS.gene031838.t1 AT4G17230 32.061 262 162 5 64 313 153 410 3.58e-25 105
MS.gene031838.t1 AT3G46600 28.185 259 169 4 66 312 1 254 6.37e-25 103
MS.gene031838.t1 AT3G46600 27.969 261 171 4 64 312 207 462 7.17e-25 105
MS.gene031838.t1 AT3G46600 28.185 259 169 4 66 312 177 430 1.47e-24 103
MS.gene031838.t1 AT2G29065 28.462 260 169 5 69 312 252 510 2.74e-24 103
MS.gene031838.t1 AT4G08250 29.323 266 170 8 55 306 93 354 2.28e-23 100
MS.gene031838.t1 AT1G55580 32.624 282 147 11 69 308 44 324 4.83e-22 96.3
MS.gene031838.t1 AT4G00150 29.958 237 134 9 70 297 209 422 2.40e-16 79.7
MS.gene031838.t1 AT3G49950 27.306 271 164 10 69 312 30 294 4.49e-16 78.6
MS.gene031838.t1 AT3G60630 39.216 102 54 2 145 238 329 430 6.38e-14 72.8
MS.gene031838.t1 AT4G36710 25.614 285 188 10 40 307 90 367 1.18e-11 65.5

Find 74 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATAACAGGAAGCGATTT+AGG 0.268170 4.3:+32270446 MS.gene031838:CDS
TCTACAGGTCTCAAACTTCT+AGG 0.268544 4.3:+32269807 MS.gene031838:CDS
GTTACCGATTTAGCAGTTAA+TGG 0.272210 4.3:-32270008 None:intergenic
CATCCGATTGAGGGTAAAAT+TGG 0.305802 4.3:+32270344 MS.gene031838:CDS
TCAACGGTCGTGATCAATTT+CGG 0.318610 4.3:-32270497 None:intergenic
TGTTCGCGTTGAGCGCGTTT+GGG 0.328048 4.3:-32269633 None:intergenic
TGATATCAAGATCAATGATA+TGG 0.341996 4.3:-32270157 None:intergenic
ATCCGCTCGGTGAGAATTAC+CGG 0.347135 4.3:+32270236 MS.gene031838:CDS
CATTGTTTGTATGATATAAC+AGG 0.349848 4.3:+32270434 MS.gene031838:CDS
AGATTCTTCTCCTTCGGTTT+CGG 0.352465 4.3:-32269787 None:intergenic
ACCTGTAGATTCTTCTCCTT+CGG 0.364116 4.3:-32269793 None:intergenic
GTGTTCGCGTTGAGCGCGTT+TGG 0.376476 4.3:-32269634 None:intergenic
ATTGATCACGACCGTTGAAC+AGG 0.382235 4.3:+32270502 MS.gene031838:CDS
AATTGGAAGTGTAACTGAAC+CGG 0.391845 4.3:+32270361 MS.gene031838:CDS
CTCGCCATTAACTGCTAAAT+CGG 0.397282 4.3:+32270004 MS.gene031838:CDS
GTTCAGTTATCTCCGGTAAA+AGG 0.402006 4.3:-32269887 None:intergenic
ACTGAACTCTCTTCACCGTT+TGG 0.403561 4.3:+32269903 MS.gene031838:CDS
CCCGAGCGAACTCGCAAAAT+CGG 0.412376 4.3:-32270306 None:intergenic
ATGAGGCAATCGTGGTTCAT+TGG 0.429274 4.3:+32270405 MS.gene031838:CDS
TCCGAGCTGTTAGAATCAAC+CGG 0.448871 4.3:+32270272 MS.gene031838:CDS
TTCGAGTTTCATCCGATTGA+GGG 0.456448 4.3:+32270335 MS.gene031838:CDS
TCGGTGAGAATTACCGGTTT+CGG 0.471801 4.3:+32270242 MS.gene031838:CDS
TGAACCACGATTGCCTCATT+CGG 0.484162 4.3:-32270401 None:intergenic
CAACGGTGAAACCGAGTTGT+AGG 0.484579 4.3:-32270069 None:intergenic
TGTTCCACATCCTTGCTTCG+AGG 0.493152 4.3:+32270204 MS.gene031838:CDS
GCAAGGATGTGGAACAATCC+TGG 0.495564 4.3:-32270197 None:intergenic
ACAAGTCTTTGAACATGAAA+CGG 0.502255 4.3:+32269761 MS.gene031838:CDS
AAAATCGGCGAGTCTTCTTC+CGG 0.502799 4.3:-32270291 None:intergenic
GTTCGAGTTTCATCCGATTG+AGG 0.506283 4.3:+32270334 MS.gene031838:CDS
TTCACCGTTTGGAACTTCAC+CGG 0.509521 4.3:+32269914 MS.gene031838:CDS
ATATCATGCAAGGACTTCAA+TGG 0.519599 4.3:+32270174 MS.gene031838:CDS
TCGACCTCGAAGCAAGGATG+TGG 0.523785 4.3:-32270208 None:intergenic
GAACGTTGAGATTGCTGACT+CGG 0.523872 4.3:+32270468 MS.gene031838:CDS
CTCGGAAGAAGATCCGAAAC+CGG 0.524772 4.3:-32270255 None:intergenic
TCCGGTTGATTCTAACAGCT+CGG 0.529075 4.3:-32270273 None:intergenic
GTTCGCGTTGAGCGCGTTTG+GGG 0.532559 4.3:-32269632 None:intergenic
GGTCTAACACCGAGTTGACC+CGG 0.536418 4.3:-32270380 None:intergenic
ATGCAAGGACTTCAATGGCC+AGG 0.537402 4.3:+32270179 MS.gene031838:CDS
TGAGATTGCTGACTCGGCTG+AGG 0.537567 4.3:+32270474 MS.gene031838:CDS
CTACAACTCGGTTTCACCGT+TGG 0.539952 4.3:+32270070 MS.gene031838:CDS
CTACAGGTCTCAAACTTCTA+GGG 0.540758 4.3:+32269808 MS.gene031838:CDS
TCTTCTTCGACCTCGAAGCA+AGG 0.546617 4.3:-32270214 None:intergenic
CGCGTTTCAATCCTACAACT+CGG 0.552518 4.3:+32270058 MS.gene031838:CDS
ACTCGGTGTTAGACCGAATG+AGG 0.563043 4.3:+32270388 MS.gene031838:CDS
GAAGAGAGTTCAGTTATCTC+CGG 0.569254 4.3:-32269894 None:intergenic
CGTGGTGGAACACGACGCAC+AGG 0.569793 4.3:+32269722 MS.gene031838:CDS
AAATACGCACCAACTCGCTC+CGG 0.573807 4.3:-32269933 None:intergenic
ACCGAAGGAGAAGAATCTAC+AGG 0.582225 4.3:+32269792 MS.gene031838:CDS
AAAGGTCGTTGGCGAAGTCG+AGG 0.582557 4.3:-32269869 None:intergenic
ATTGATCTTGATATCATGCA+AGG 0.582635 4.3:+32270164 MS.gene031838:CDS
ATTGGAAGTGTAACTGAACC+GGG 0.584733 4.3:+32270362 MS.gene031838:CDS
CGCTCCGGTGAAGTTCCAAA+CGG 0.584887 4.3:-32269918 None:intergenic
GAACAGGATCTTAGCCATGC+TGG 0.589797 4.3:+32270518 MS.gene031838:CDS
ATGTGCTGAGTGTGTTGCCA+TGG 0.593544 4.3:+32269842 MS.gene031838:CDS
GGAACTTCACCGGAGCGAGT+TGG 0.594130 4.3:+32269924 MS.gene031838:CDS
GGCGAAGTCGAGGTTATCCA+TGG 0.595794 4.3:-32269859 None:intergenic
CTGTGCGTCGTGTTCCACCA+CGG 0.597321 4.3:-32269721 None:intergenic
GAAACGGAAACCGAAACCGA+AGG 0.606138 4.3:+32269777 MS.gene031838:CDS
ATGCTGGTAGCTTCTTAGCG+AGG 0.608930 4.3:+32270534 MS.gene031838:CDS
TATCTCCGGTAAAAGGTCGT+TGG 0.618446 4.3:-32269880 None:intergenic
GAAAGATGGTTCATGAACGT+TGG 0.619264 4.3:-32269670 None:intergenic
ACATAAGCAATTAAATCAGA+CGG 0.619937 4.3:-32270908 None:intergenic
CGTGTGGTGAGTTCTTGTCT+CGG 0.629592 4.3:+32269975 MS.gene031838:CDS
GTAACTGAACCGGGTCAACT+CGG 0.642976 4.3:+32270371 MS.gene031838:CDS
ACAAGCGTTACAAGCGCGTG+TGG 0.650613 4.3:+32269959 MS.gene031838:CDS
ATGGCTAAGATCCTGTTCAA+CGG 0.673618 4.3:-32270513 None:intergenic
CTCTAGTGAGAAAACCGTGG+TGG 0.676760 4.3:+32269707 MS.gene031838:CDS
TCGCTAAGAAGCTACCAGCA+TGG 0.677504 4.3:-32270532 None:intergenic
TAGACCGAATGAGGCAATCG+TGG 0.677791 4.3:+32270397 MS.gene031838:CDS
AAGTGTGAGAATTTCACCAA+CGG 0.681780 4.3:-32270086 None:intergenic
GAGGTCGAAGAAGATCCGCT+CGG 0.688733 4.3:+32270223 MS.gene031838:CDS
AACCGGTAATTCTCACCGAG+CGG 0.697234 4.3:-32270238 None:intergenic
CTACTCTAGTGAGAAAACCG+TGG 0.700727 4.3:+32269704 MS.gene031838:CDS
TCAATCGGATGAAACTCGAA+CGG 0.709737 4.3:-32270332 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CATTGTTTGTATGATATAAC+AGG + chr4.3:32270434-32270453 MS.gene031838:CDS 25.0%
! TGATATCAAGATCAATGATA+TGG - chr4.3:32270160-32270179 None:intergenic 25.0%
ACAAGTCTTTGAACATGAAA+CGG + chr4.3:32269761-32269780 MS.gene031838:CDS 30.0%
ATTGATCTTGATATCATGCA+AGG + chr4.3:32270164-32270183 MS.gene031838:CDS 30.0%
!! TAGTTAGGTCTTTTGAAAGA+TGG - chr4.3:32269687-32269706 None:intergenic 30.0%
AAGTGTGAGAATTTCACCAA+CGG - chr4.3:32270089-32270108 None:intergenic 35.0%
ATAAGGAACATTATCGCTGT+TGG + chr4.3:32270668-32270687 MS.gene031838:intron 35.0%
ATATCATGCAAGGACTTCAA+TGG + chr4.3:32270174-32270193 MS.gene031838:CDS 35.0%
ATTTCGACCCGTTTCATTAA+GGG + chr4.3:32270754-32270773 MS.gene031838:intron 35.0%
GATATAACAGGAAGCGATTT+AGG + chr4.3:32270446-32270465 MS.gene031838:CDS 35.0%
TATACATTGGTGGAAGAGAA+TGG + chr4.3:32270830-32270849 MS.gene031838:intron 35.0%
! AATTGGAAGTGTAACTGAAC+CGG + chr4.3:32270361-32270380 MS.gene031838:CDS 35.0%
! CTTCCAATTTTACCCTCAAT+CGG - chr4.3:32270350-32270369 None:intergenic 35.0%
! GTTTTCTCACTAGAGTAGTT+AGG - chr4.3:32269702-32269721 None:intergenic 35.0%
! TGCGAATCAAGCAATTTTTC+AGG + chr4.3:32270112-32270131 MS.gene031838:CDS 35.0%
!! GTTACCGATTTAGCAGTTAA+TGG - chr4.3:32270011-32270030 None:intergenic 35.0%
!!! TCAAGCAATTTTTCAGGCTT+TGG + chr4.3:32270118-32270137 MS.gene031838:CDS 35.0%
ACCAATGTATATCCTCTCCA+AGG - chr4.3:32270821-32270840 None:intergenic 40.0%
ACCTGTAGATTCTTCTCCTT+CGG - chr4.3:32269796-32269815 None:intergenic 40.0%
AGATTCTTCTCCTTCGGTTT+CGG - chr4.3:32269790-32269809 None:intergenic 40.0%
ATGGCTAAGATCCTGTTCAA+CGG - chr4.3:32270516-32270535 None:intergenic 40.0%
CATCCGATTGAGGGTAAAAT+TGG + chr4.3:32270344-32270363 MS.gene031838:CDS 40.0%
CTACAGGTCTCAAACTTCTA+GGG + chr4.3:32269808-32269827 MS.gene031838:CDS 40.0%
CTCGCCATTAACTGCTAAAT+CGG + chr4.3:32270004-32270023 MS.gene031838:CDS 40.0%
GAAAGATGGTTCATGAACGT+TGG - chr4.3:32269673-32269692 None:intergenic 40.0%
GAAGAGAGTTCAGTTATCTC+CGG - chr4.3:32269897-32269916 None:intergenic 40.0%
GATTTCGACCCGTTTCATTA+AGG + chr4.3:32270753-32270772 MS.gene031838:intron 40.0%
GTTCAGTTATCTCCGGTAAA+AGG - chr4.3:32269890-32269909 None:intergenic 40.0%
TAATGAAACGGGTCGAAATC+CGG - chr4.3:32270753-32270772 None:intergenic 40.0%
TCAACGGTCGTGATCAATTT+CGG - chr4.3:32270500-32270519 None:intergenic 40.0%
TCAATCGGATGAAACTCGAA+CGG - chr4.3:32270335-32270354 None:intergenic 40.0%
TCCTTGGAGAGGATATACAT+TGG + chr4.3:32270817-32270836 MS.gene031838:intron 40.0%
TCTACAGGTCTCAAACTTCT+AGG + chr4.3:32269807-32269826 MS.gene031838:CDS 40.0%
TGAAGTGAAAGTTGAGAGGT+GGG + chr4.3:32270709-32270728 MS.gene031838:intron 40.0%
TTGGAGAGGATATACATTGG+TGG + chr4.3:32270820-32270839 MS.gene031838:intron 40.0%
TTTCGACCCGTTTCATTAAG+GGG + chr4.3:32270755-32270774 MS.gene031838:intron 40.0%
! ATTGGAAGTGTAACTGAACC+GGG + chr4.3:32270362-32270381 MS.gene031838:CDS 40.0%
! CTTTGTTGATTGCTTCTGCT+TGG + chr4.3:32270882-32270901 MS.gene031838:intron 40.0%
! TTCGAGTTTCATCCGATTGA+GGG + chr4.3:32270335-32270354 MS.gene031838:CDS 40.0%
!!! GCAATTTTTCAGGCTTTGGA+TGG + chr4.3:32270122-32270141 MS.gene031838:CDS 40.0%
AAAATCGGCGAGTCTTCTTC+CGG - chr4.3:32270294-32270313 None:intergenic 45.0%
AAGTGAAAGTTGAGAGGTGG+GGG + chr4.3:32270711-32270730 MS.gene031838:intron 45.0%
ACCGAAGGAGAAGAATCTAC+AGG + chr4.3:32269792-32269811 MS.gene031838:CDS 45.0%
ACTGAACTCTCTTCACCGTT+TGG + chr4.3:32269903-32269922 MS.gene031838:CDS 45.0%
AGGAACATTATCGCTGTTGG+TGG + chr4.3:32270671-32270690 MS.gene031838:intron 45.0%
ATGAGGCAATCGTGGTTCAT+TGG + chr4.3:32270405-32270424 MS.gene031838:CDS 45.0%
ATGGTTCTCTCAAGCTTGGT+TGG + chr4.3:32270849-32270868 MS.gene031838:intron 45.0%
ATTGATCACGACCGTTGAAC+AGG + chr4.3:32270502-32270521 MS.gene031838:CDS 45.0%
CGCGTTTCAATCCTACAACT+CGG + chr4.3:32270058-32270077 MS.gene031838:CDS 45.0%
CGGGTTACCCCTTAATGAAA+CGG - chr4.3:32270765-32270784 None:intergenic 45.0%
CGTTTCATTAAGGGGTAACC+CGG + chr4.3:32270763-32270782 MS.gene031838:intron 45.0%
CTACTCTAGTGAGAAAACCG+TGG + chr4.3:32269704-32269723 MS.gene031838:CDS 45.0%
CTGGTGAAGTGAAAGTTGAG+AGG + chr4.3:32270705-32270724 MS.gene031838:intron 45.0%
GAAGTGAAAGTTGAGAGGTG+GGG + chr4.3:32270710-32270729 MS.gene031838:intron 45.0%
GAGAATGGTTCTCTCAAGCT+TGG + chr4.3:32270845-32270864 MS.gene031838:intron 45.0%
GGAACATTATCGCTGTTGGT+GGG + chr4.3:32270672-32270691 MS.gene031838:intron 45.0%
GGGTTACCCCTTAATGAAAC+GGG - chr4.3:32270764-32270783 None:intergenic 45.0%
GTGAAGTGAAAGTTGAGAGG+TGG + chr4.3:32270708-32270727 MS.gene031838:intron 45.0%
TAGCTTCTTAGCGAGGTTTG+TGG + chr4.3:32270541-32270560 MS.gene031838:intron 45.0%
TATCTCCGGTAAAAGGTCGT+TGG - chr4.3:32269883-32269902 None:intergenic 45.0%
TCCGAGCTGTTAGAATCAAC+CGG + chr4.3:32270272-32270291 MS.gene031838:CDS 45.0%
TGAACCACGATTGCCTCATT+CGG - chr4.3:32270404-32270423 None:intergenic 45.0%
TGTTGTTGGGGATGTTTCCT+TGG + chr4.3:32270801-32270820 MS.gene031838:intron 45.0%
TTCACTTCACCAGTCCTCTT+AGG - chr4.3:32270698-32270717 None:intergenic 45.0%
TTCTCTCAAGCTTGGTTGGA+AGG + chr4.3:32270853-32270872 MS.gene031838:intron 45.0%
! GAACGTTGAGATTGCTGACT+CGG + chr4.3:32270468-32270487 MS.gene031838:CDS 45.0%
! GGATAGTGTTGAGAGGCATA+TGG + chr4.3:32270619-32270638 MS.gene031838:intron 45.0%
! GTTCGAGTTTCATCCGATTG+AGG + chr4.3:32270334-32270353 MS.gene031838:CDS 45.0%
! TAGTGTTGAGAGGCATATGG+TGG + chr4.3:32270622-32270641 MS.gene031838:intron 45.0%
! TCCGGTTGATTCTAACAGCT+CGG - chr4.3:32270276-32270295 None:intergenic 45.0%
! TTCACCGTTTGGAACTTCAC+CGG + chr4.3:32269914-32269933 MS.gene031838:CDS 45.0%
!! TCGGTGAGAATTACCGGTTT+CGG + chr4.3:32270242-32270261 MS.gene031838:CDS 45.0%
!! TCTCAGGCGAGTTTGTTGTT+GGG + chr4.3:32270788-32270807 MS.gene031838:intron 45.0%
!! TTCTCAGGCGAGTTTGTTGT+TGG + chr4.3:32270787-32270806 MS.gene031838:intron 45.0%
AAATACGCACCAACTCGCTC+CGG - chr4.3:32269936-32269955 None:intergenic 50.0%
AACAAACTCGCCTGAGAAGC+CGG - chr4.3:32270785-32270804 None:intergenic 50.0%
AACCGGTAATTCTCACCGAG+CGG - chr4.3:32270241-32270260 None:intergenic 50.0%
AGCTGTTGGGGTGTGAGATA+AGG + chr4.3:32270651-32270670 MS.gene031838:intron 50.0%
AGTGAAAGTTGAGAGGTGGG+GGG + chr4.3:32270712-32270731 MS.gene031838:intron 50.0%
ATGCAAGGACTTCAATGGCC+AGG + chr4.3:32270179-32270198 MS.gene031838:CDS 50.0%
ATGCTGGTAGCTTCTTAGCG+AGG + chr4.3:32270534-32270553 MS.gene031838:CDS 50.0%
ATGTGCTGAGTGTGTTGCCA+TGG + chr4.3:32269842-32269861 MS.gene031838:CDS 50.0%
CAACGGTGAAACCGAGTTGT+AGG - chr4.3:32270072-32270091 None:intergenic 50.0%
CGTGTGGTGAGTTCTTGTCT+CGG + chr4.3:32269975-32269994 MS.gene031838:CDS 50.0%
CTACAACTCGGTTTCACCGT+TGG + chr4.3:32270070-32270089 MS.gene031838:CDS 50.0%
CTCGGAAGAAGATCCGAAAC+CGG - chr4.3:32270258-32270277 None:intergenic 50.0%
CTCTAGTGAGAAAACCGTGG+TGG + chr4.3:32269707-32269726 MS.gene031838:CDS 50.0%
CTTCTTAGCGAGGTTTGTGG+AGG + chr4.3:32270544-32270563 MS.gene031838:intron 50.0%
GAAACGGAAACCGAAACCGA+AGG + chr4.3:32269777-32269796 MS.gene031838:CDS 50.0%
GAACAGGATCTTAGCCATGC+TGG + chr4.3:32270518-32270537 MS.gene031838:CDS 50.0%
GCAAGGATGTGGAACAATCC+TGG - chr4.3:32270200-32270219 None:intergenic 50.0%
GTAACTGAACCGGGTCAACT+CGG + chr4.3:32270371-32270390 MS.gene031838:CDS 50.0%
TAGACCGAATGAGGCAATCG+TGG + chr4.3:32270397-32270416 MS.gene031838:CDS 50.0%
TCGCTAAGAAGCTACCAGCA+TGG - chr4.3:32270535-32270554 None:intergenic 50.0%
TCTTCTTCGACCTCGAAGCA+AGG - chr4.3:32270217-32270236 None:intergenic 50.0%
TGTTCCACATCCTTGCTTCG+AGG + chr4.3:32270204-32270223 MS.gene031838:CDS 50.0%
TTGGGGATGTTTCCTTGGAG+AGG + chr4.3:32270806-32270825 MS.gene031838:intron 50.0%
! ATCCGCTCGGTGAGAATTAC+CGG + chr4.3:32270236-32270255 MS.gene031838:CDS 50.0%
! CTTCGCCAACGACCTTTTAC+CGG + chr4.3:32269875-32269894 MS.gene031838:CDS 50.0%
! TGGGTGTGGATAGTGTTGAG+AGG + chr4.3:32270612-32270631 MS.gene031838:intron 50.0%
!! ACTCGGTGTTAGACCGAATG+AGG + chr4.3:32270388-32270407 MS.gene031838:CDS 50.0%
!! CTCAGGCGAGTTTGTTGTTG+GGG + chr4.3:32270789-32270808 MS.gene031838:intron 50.0%
AAAGGTCGTTGGCGAAGTCG+AGG - chr4.3:32269872-32269891 None:intergenic 55.0%
ACAAACTCGCCTGAGAAGCC+GGG - chr4.3:32270784-32270803 None:intergenic 55.0%
ACAAGCGTTACAAGCGCGTG+TGG + chr4.3:32269959-32269978 MS.gene031838:CDS 55.0%
CCCGAGCGAACTCGCAAAAT+CGG - chr4.3:32270309-32270328 None:intergenic 55.0%
CGCTCCGGTGAAGTTCCAAA+CGG - chr4.3:32269921-32269940 None:intergenic 55.0%
GAGGTCGAAGAAGATCCGCT+CGG + chr4.3:32270223-32270242 MS.gene031838:CDS 55.0%
GGCGAAGTCGAGGTTATCCA+TGG - chr4.3:32269862-32269881 None:intergenic 55.0%
TCGACCTCGAAGCAAGGATG+TGG - chr4.3:32270211-32270230 None:intergenic 55.0%
TGTTCGCGTTGAGCGCGTTT+GGG - chr4.3:32269636-32269655 None:intergenic 55.0%
! ATGGTGGAGCAGCAGCTGTT+GGG + chr4.3:32270638-32270657 MS.gene031838:intron 55.0%
! CCGATTTTGCGAGTTCGCTC+GGG + chr4.3:32270306-32270325 MS.gene031838:CDS 55.0%
! GGTCTAACACCGAGTTGACC+CGG - chr4.3:32270383-32270402 None:intergenic 55.0%
! GGTGGGGGGATGAGTTGAAA+CGG + chr4.3:32270726-32270745 MS.gene031838:intron 55.0%
! GTGGGGGGATGAGTTGAAAC+GGG + chr4.3:32270727-32270746 MS.gene031838:intron 55.0%
! TATGGTGGAGCAGCAGCTGT+TGG + chr4.3:32270637-32270656 MS.gene031838:intron 55.0%
! TCTAGGGGATGGTTTGGGTG+TGG + chr4.3:32270598-32270617 MS.gene031838:intron 55.0%
!! GACGCTCTAGGGGATGGTTT+GGG + chr4.3:32270593-32270612 MS.gene031838:intron 55.0%
!! GCCGATTTTGCGAGTTCGCT+CGG + chr4.3:32270305-32270324 MS.gene031838:CDS 55.0%
!! TGAGATTGCTGACTCGGCTG+AGG + chr4.3:32270474-32270493 MS.gene031838:CDS 55.0%
!! TTGTTCGACGCTCTAGGGGA+TGG + chr4.3:32270587-32270606 MS.gene031838:intron 55.0%
AAGGGGTAACCCGGCTTCTC+AGG + chr4.3:32270772-32270791 MS.gene031838:intron 60.0%
AGCGCGTTGTTCGACGCTCT+AGG + chr4.3:32270581-32270600 MS.gene031838:intron 60.0%
CGCGTTGTTCGACGCTCTAG+GGG + chr4.3:32270583-32270602 MS.gene031838:intron 60.0%
CTGTGCGTCGTGTTCCACCA+CGG - chr4.3:32269724-32269743 None:intergenic 60.0%
GCGCGTTGTTCGACGCTCTA+GGG + chr4.3:32270582-32270601 MS.gene031838:intron 60.0%
GGAACTTCACCGGAGCGAGT+TGG + chr4.3:32269924-32269943 MS.gene031838:CDS 60.0%
GTGTTCGCGTTGAGCGCGTT+TGG - chr4.3:32269637-32269656 None:intergenic 60.0%
GTTCGCGTTGAGCGCGTTTG+GGG - chr4.3:32269635-32269654 None:intergenic 60.0%
TGGTGGAGCAGCAGCTGTTG+GGG + chr4.3:32270639-32270658 MS.gene031838:intron 60.0%
! GGGGATGAGTTGAAACGGGC+CGG + chr4.3:32270731-32270750 MS.gene031838:intron 60.0%
! GTTGGTGGGCCTAAGAGGAC+TGG + chr4.3:32270686-32270705 MS.gene031838:intron 60.0%
! TCGCTGTTGGTGGGCCTAAG+AGG + chr4.3:32270681-32270700 MS.gene031838:intron 60.0%
!! CGACGCTCTAGGGGATGGTT+TGG + chr4.3:32270592-32270611 MS.gene031838:intron 60.0%
! CGTGGTGGAACACGACGCAC+AGG + chr4.3:32269722-32269741 MS.gene031838:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 32269627 32270920 32269627 ID=MS.gene031838
chr4.3 mRNA 32269627 32270920 32269627 ID=MS.gene031838.t1;Parent=MS.gene031838
chr4.3 exon 32269627 32270555 32269627 ID=MS.gene031838.t1.exon1;Parent=MS.gene031838.t1
chr4.3 CDS 32269627 32270555 32269627 ID=cds.MS.gene031838.t1;Parent=MS.gene031838.t1
chr4.3 exon 32270908 32270920 32270908 ID=MS.gene031838.t1.exon2;Parent=MS.gene031838.t1
chr4.3 CDS 32270908 32270920 32270908 ID=cds.MS.gene031838.t1;Parent=MS.gene031838.t1
Gene Sequence

>MS.gene031838

ATGAACCCCAAACGCGCTCAACGCGAACACGATGACGACGACACCAACGTTCATGAACCATCTTTCAAAAGACCTAACTACTCTAGTGAGAAAACCGTGGTGGAACACGACGCACAGGACGACGAACAACAACAACAAGTCTTTGAACATGAAACGGAAACCGAAACCGAAGGAGAAGAATCTACAGGTCTCAAACTTCTAGGGCTTTTACTTCAATGTGCTGAGTGTGTTGCCATGGATAACCTCGACTTCGCCAACGACCTTTTACCGGAGATAACTGAACTCTCTTCACCGTTTGGAACTTCACCGGAGCGAGTTGGTGCGTATTTTGCACAAGCGTTACAAGCGCGTGTGGTGAGTTCTTGTCTCGGAAGCTACTCGCCATTAACTGCTAAATCGGTAACGCTAAATCAGTCTCAGAGAATCTTCAACGCGTTTCAATCCTACAACTCGGTTTCACCGTTGGTGAAATTCTCACACTTCACTGCGAATCAAGCAATTTTTCAGGCTTTGGATGGTGAAGATCGTGTCCATATCATTGATCTTGATATCATGCAAGGACTTCAATGGCCAGGATTGTTCCACATCCTTGCTTCGAGGTCGAAGAAGATCCGCTCGGTGAGAATTACCGGTTTCGGATCTTCTTCCGAGCTGTTAGAATCAACCGGAAGAAGACTCGCCGATTTTGCGAGTTCGCTCGGGTTACCGTTCGAGTTTCATCCGATTGAGGGTAAAATTGGAAGTGTAACTGAACCGGGTCAACTCGGTGTTAGACCGAATGAGGCAATCGTGGTTCATTGGATGCATCATTGTTTGTATGATATAACAGGAAGCGATTTAGGAACGTTGAGATTGCTGACTCGGCTGAGGCCGAAATTGATCACGACCGTTGAACAGGATCTTAGCCATGCTGGTAGCTTCTTAGCGAGGTTTGTGGAGGCTTTGCATTATTACAGCGCGTTGTTCGACGCTCTAGGGGATGGTTTGGGTGTGGATAGTGTTGAGAGGCATATGGTGGAGCAGCAGCTGTTGGGGTGTGAGATAAGGAACATTATCGCTGTTGGTGGGCCTAAGAGGACTGGTGAAGTGAAAGTTGAGAGGTGGGGGGATGAGTTGAAACGGGCCGGATTTCGACCCGTTTCATTAAGGGGTAACCCGGCTTCTCAGGCGAGTTTGTTGTTGGGGATGTTTCCTTGGAGAGGATATACATTGGTGGAAGAGAATGGTTCTCTCAAGCTTGGTTGGAAGGATCTCTCTTTGTTGATTGCTTCTGCTTGGCAGCCGTCTGATTTAA
Protein sequence

>MS.gene031838.t1

MNPKRAQREHDDDDTNVHEPSFKRPNYSSEKTVVEHDAQDDEQQQQVFEHETETETEGEESTGLKLLGLLLQCAECVAMDNLDFANDLLPEITELSSPFGTSPERVGAYFAQALQARVVSSCLGSYSPLTAKSVTLNQSQRIFNAFQSYNSVSPLVKFSHFTANQAIFQALDGEDRVHIIDLDIMQGLQWPGLFHILASRSKKIRSVRITGFGSSSELLESTGRRLADFASSLGLPFEFHPIEGKIGSVTEPGQLGVRPNEAIVVHWMHHCLYDITGSDLGTLRLLTRLRPKLITTVEQDLSHAGSFLASRLI