Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031851.t1 | XP_024635100.1 | 92.9 | 170 | 12 | 0 | 4 | 173 | 214 | 383 | 1.80E-86 | 328.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031851.t1 | Q7XJS0 | 61.0 | 164 | 63 | 1 | 6 | 168 | 183 | 346 | 1.2e-54 | 214.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031851.t1 | G7J3L4 | 92.3 | 168 | 13 | 0 | 4 | 171 | 177 | 344 | 2.4e-85 | 324.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene031851.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031851.t1 | MTR_3g073000 | 92.262 | 168 | 13 | 0 | 4 | 171 | 177 | 344 | 4.56e-114 | 329 |
| MS.gene031851.t1 | MTR_3g073000 | 92.262 | 168 | 13 | 0 | 4 | 171 | 177 | 344 | 2.32e-113 | 330 |
| MS.gene031851.t1 | MTR_3g073000 | 72.024 | 168 | 12 | 1 | 4 | 171 | 177 | 309 | 2.02e-80 | 245 |
| MS.gene031851.t1 | MTR_3g093440 | 28.205 | 156 | 95 | 4 | 9 | 162 | 459 | 599 | 5.80e-13 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031851.t1 | AT2G17900 | 60.976 | 164 | 63 | 1 | 6 | 168 | 188 | 351 | 1.93e-68 | 216 |
Find 31 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTACTAACCCACATTCT+TGG | 0.284514 | 3.4:+71818804 | None:intergenic |
| GGTTTCTTGCTCCGCACAAC+TGG | 0.393388 | 3.4:-71819004 | MS.gene031851:intron |
| CGAGAAAATGCAATTCTAGA+AGG | 0.401251 | 3.4:-71819055 | MS.gene031851:CDS |
| TGGTTACCTGTGATTGATAA+TGG | 0.407535 | 3.4:+71820115 | None:intergenic |
| TGACAGTGAGTTAAGACCTC+TGG | 0.417510 | 3.4:-71820162 | MS.gene031851:CDS |
| GAGTTAAGACCTCTGGGAAC+AGG | 0.450124 | 3.4:-71820155 | MS.gene031851:CDS |
| GGGGACAAACACAAGTGAAT+AGG | 0.464095 | 3.4:+71819222 | None:intergenic |
| CTGTGCAACATGTGCCTAAA+GGG | 0.466513 | 3.4:-71819957 | MS.gene031851:CDS |
| ATAGATACCTCAGTCCCTTT+AGG | 0.485587 | 3.4:+71819943 | None:intergenic |
| TTGTTTCCATTATCAATCAC+AGG | 0.492390 | 3.4:-71820121 | MS.gene031851:intron |
| TCTTAACTCACTGTCACAAA+TGG | 0.513188 | 3.4:+71820169 | None:intergenic |
| GCATTTCAATGCCAAGAATG+TGG | 0.517194 | 3.4:-71818815 | MS.gene031851:CDS |
| AAGCACTGTGACTCGACAAA+AGG | 0.521126 | 3.4:-71819261 | MS.gene031851:CDS |
| TTGTCCCCTCTGTTCTAAAG+TGG | 0.523368 | 3.4:-71819207 | MS.gene031851:intron |
| ATAAGTTATATAGAGACAGC+TGG | 0.532422 | 3.4:-71819284 | MS.gene031851:CDS |
| AGGATACTGCCACTAGAAGA+AGG | 0.532430 | 3.4:+71818719 | None:intergenic |
| AAGTACCACTTTAGAACAGA+GGG | 0.532968 | 3.4:+71819202 | None:intergenic |
| CTGCCTACCCAATTCAGTGT+TGG | 0.538934 | 3.4:-71820010 | MS.gene031851:CDS |
| GCTTCTAAGCCTTCTTCTAG+TGG | 0.547425 | 3.4:-71818728 | MS.gene031851:CDS |
| GACAGTGAGTTAAGACCTCT+GGG | 0.564404 | 3.4:-71820161 | MS.gene031851:CDS |
| TGATTGATAATGGAAACAAC+AGG | 0.566394 | 3.4:+71820125 | None:intergenic |
| CATTTCAATGCCAAGAATGT+GGG | 0.570052 | 3.4:-71818814 | MS.gene031851:CDS |
| GCGCATTACATGCAAACTGC+AGG | 0.589514 | 3.4:+71820195 | None:intergenic |
| GCTGTGCAACATGTGCCTAA+AGG | 0.597001 | 3.4:-71819958 | MS.gene031851:CDS |
| ATGTGGGTTAGTAAGAGACA+AGG | 0.627932 | 3.4:-71818798 | MS.gene031851:CDS |
| TTTAGGTTATACCAGTTGTG+CGG | 0.631225 | 3.4:+71818993 | None:intergenic |
| AAACCAACACTGAATTGGGT+AGG | 0.637837 | 3.4:+71820007 | None:intergenic |
| ACATGTGCCTAAAGGGACTG+AGG | 0.674349 | 3.4:-71819950 | MS.gene031851:intron |
| TAAGTACCACTTTAGAACAG+AGG | 0.686937 | 3.4:+71819201 | None:intergenic |
| GGATACAATCCTGTTCCCAG+AGG | 0.760315 | 3.4:+71820146 | None:intergenic |
| AGTACCACTTTAGAACAGAG+GGG | 0.783095 | 3.4:+71819203 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAATTTAAAACTATTTGA+CGG | - | chr3.4:71819539-71819558 | MS.gene031851:intron | 10.0% |
| !!! | TTTTGTTTTTAAATTATAGA+TGG | - | chr3.4:71820085-71820104 | MS.gene031851:intron | 10.0% |
| !!! | TCAGTCTTATATTTATAAAA+AGG | + | chr3.4:71820015-71820034 | None:intergenic | 15.0% |
| !!! | TGTCATTTAAAGATTATTAA+TGG | - | chr3.4:71819077-71819096 | MS.gene031851:intron | 15.0% |
| !! | CATAGTAATTTCAGTAAAAT+TGG | - | chr3.4:71819791-71819810 | MS.gene031851:intron | 20.0% |
| !! | CTTATATTTATAAAAAGGAG+AGG | + | chr3.4:71820010-71820029 | None:intergenic | 20.0% |
| !! | TAAAATTGGAATGTTATCAT+TGG | - | chr3.4:71819805-71819824 | MS.gene031851:intron | 20.0% |
| !!! | AATCTGAAATATTTTCTTGT+TGG | - | chr3.4:71819760-71819779 | MS.gene031851:intron | 20.0% |
| !!! | GTATAGTAAGACATTATTTT+CGG | + | chr3.4:71818856-71818875 | None:intergenic | 20.0% |
| !!! | TTTTGTAAAAGTTTCAGTTA+GGG | - | chr3.4:71819478-71819497 | MS.gene031851:intron | 20.0% |
| !!! | TTTTTGTAAAAGTTTCAGTT+AGG | - | chr3.4:71819477-71819496 | MS.gene031851:intron | 20.0% |
| ! | GGAACAAAAAATAAGTTAAG+TGG | + | chr3.4:71818835-71818854 | None:intergenic | 25.0% |
| !! | AAGATTATGATGTTCCTTTT+AGG | + | chr3.4:71819954-71819973 | None:intergenic | 25.0% |
| !! | ATTTAAAACTATTTGACGGA+TGG | - | chr3.4:71819543-71819562 | MS.gene031851:intron | 25.0% |
| !!! | TCAGTTAAGTTAGTTTTGAA+GGG | - | chr3.4:71819833-71819852 | MS.gene031851:intron | 25.0% |
| !!! | TTCAGTTAAGTTAGTTTTGA+AGG | - | chr3.4:71819832-71819851 | MS.gene031851:intron | 25.0% |
| AACGAACTGAATATTGCAAA+TGG | + | chr3.4:71819039-71819058 | None:intergenic | 30.0% | |
| AGCAAGTATTAAACTTGATG+TGG | + | chr3.4:71819597-71819616 | None:intergenic | 30.0% | |
| ATAAGTTATATAGAGACAGC+TGG | - | chr3.4:71819643-71819662 | MS.gene031851:intron | 30.0% | |
| ATGGAAACTCAGCAAAATTT+AGG | - | chr3.4:71819096-71819115 | MS.gene031851:intron | 30.0% | |
| ATTCAGTTCGTTTGTTTACA+GGG | - | chr3.4:71819047-71819066 | MS.gene031851:CDS | 30.0% | |
| CAAAATTTAGGCATAGTGTT+AGG | - | chr3.4:71819108-71819127 | MS.gene031851:intron | 30.0% | |
| CCATATTGTTTCGTAATTGT+AGG | - | chr3.4:71819137-71819156 | MS.gene031851:intron | 30.0% | |
| CCTACAATTACGAAACAATA+TGG | + | chr3.4:71819140-71819159 | None:intergenic | 30.0% | |
| TAGCATTTGTATGCCAATTT+CGG | - | chr3.4:71819428-71819447 | MS.gene031851:intron | 30.0% | |
| TATTCAGTTCGTTTGTTTAC+AGG | - | chr3.4:71819046-71819065 | MS.gene031851:CDS | 30.0% | |
| TCAAAAACCAACACTGAATT+GGG | + | chr3.4:71818927-71818946 | None:intergenic | 30.0% | |
| TGCAAAAATGAAACATGTGA+TGG | - | chr3.4:71819902-71819921 | MS.gene031851:intron | 30.0% | |
| TTCAAAAACCAACACTGAAT+TGG | + | chr3.4:71818928-71818947 | None:intergenic | 30.0% | |
| TTCTACATATCTACAAGCAT+AGG | - | chr3.4:71819223-71819242 | MS.gene031851:CDS | 30.0% | |
| TTGTTTCCATTATCAATCAC+AGG | - | chr3.4:71818806-71818825 | MS.gene031851:CDS | 30.0% | |
| TTTCAAATTCAAAGAAACCC+TGG | + | chr3.4:71819180-71819199 | None:intergenic | 30.0% | |
| ! | AGTTTTCCACAAATTTGTAG+AGG | + | chr3.4:71819400-71819419 | None:intergenic | 30.0% |
| ! | ATTTTCCATTGTGAATTCCA+GGG | - | chr3.4:71819160-71819179 | MS.gene031851:intron | 30.0% |
| ! | GGAACATCATAATCTTTTAC+TGG | - | chr3.4:71819958-71819977 | MS.gene031851:CDS | 30.0% |
| !! | TGATTGATAATGGAAACAAC+AGG | + | chr3.4:71818805-71818824 | None:intergenic | 30.0% |
| !!! | AATTCAGTGTTGGTTTTTGA+AGG | - | chr3.4:71818927-71818946 | MS.gene031851:intron | 30.0% |
| AAGATATCTCCACTTTGAAC+TGG | - | chr3.4:71819298-71819317 | MS.gene031851:intron | 35.0% | |
| AGATATCTCCACTTTGAACT+GGG | - | chr3.4:71819299-71819318 | MS.gene031851:intron | 35.0% | |
| ATTCTGCCTCTACAAATTTG+TGG | - | chr3.4:71819391-71819410 | MS.gene031851:intron | 35.0% | |
| CACAACTGGTATAACCTAAA+AGG | - | chr3.4:71819937-71819956 | MS.gene031851:intron | 35.0% | |
| CATTTCAATGCCAAGAATGT+GGG | - | chr3.4:71820113-71820132 | MS.gene031851:intron | 35.0% | |
| CGAGAAAATGCAATTCTAGA+AGG | - | chr3.4:71819872-71819891 | MS.gene031851:intron | 35.0% | |
| TCTTAACTCACTGTCACAAA+TGG | + | chr3.4:71818761-71818780 | None:intergenic | 35.0% | |
| TGACAACTATCATCACCTAT+TGG | + | chr3.4:71819516-71819535 | None:intergenic | 35.0% | |
| TGGTTACCTGTGATTGATAA+TGG | + | chr3.4:71818815-71818834 | None:intergenic | 35.0% | |
| TTCCACTTATATCCTCAAAG+AGG | + | chr3.4:71819351-71819370 | None:intergenic | 35.0% | |
| ! | GATTTTCCATTGTGAATTCC+AGG | - | chr3.4:71819159-71819178 | MS.gene031851:intron | 35.0% |
| ! | TTTAGGTTATACCAGTTGTG+CGG | + | chr3.4:71819937-71819956 | None:intergenic | 35.0% |
| !! | AAAAGAGTGTCAGTAACACT+TGG | + | chr3.4:71820065-71820084 | None:intergenic | 35.0% |
| !! | AAGTACCACTTTAGAACAGA+GGG | + | chr3.4:71819728-71819747 | None:intergenic | 35.0% |
| !! | CTATGAGTTTTGTAGTCTGA+TGG | - | chr3.4:71819981-71820000 | MS.gene031851:CDS | 35.0% |
| !! | TAAGTACCACTTTAGAACAG+AGG | + | chr3.4:71819729-71819748 | None:intergenic | 35.0% |
| !!! | AGTGTTGGTTTTTGAAGGAA+GGG | - | chr3.4:71818932-71818951 | MS.gene031851:intron | 35.0% |
| AAACCAACACTGAATTGGGT+AGG | + | chr3.4:71818923-71818942 | None:intergenic | 40.0% | |
| AGAAACCCTGGAATTCACAA+TGG | + | chr3.4:71819168-71819187 | None:intergenic | 40.0% | |
| ATAGATACCTCAGTCCCTTT+AGG | + | chr3.4:71818987-71819006 | None:intergenic | 40.0% | |
| CTCTTACTAACCCACATTCT+TGG | + | chr3.4:71820126-71820145 | None:intergenic | 40.0% | |
| GCATTTCAATGCCAAGAATG+TGG | - | chr3.4:71820112-71820131 | MS.gene031851:intron | 40.0% | |
| GGGTACATCTCATATCCAAT+AGG | - | chr3.4:71819498-71819517 | MS.gene031851:intron | 40.0% | |
| ! | AAGTTGCAACTGCCTAGTTT+TGG | + | chr3.4:71819454-71819473 | None:intergenic | 40.0% |
| ! | GACCTCTTTGAGGATATAAG+TGG | - | chr3.4:71819346-71819365 | MS.gene031851:intron | 40.0% |
| !! | AGTACCACTTTAGAACAGAG+GGG | + | chr3.4:71819727-71819746 | None:intergenic | 40.0% |
| !! | ATGTGGGTTAGTAAGAGACA+AGG | - | chr3.4:71820129-71820148 | MS.gene031851:CDS | 40.0% |
| !! | TTTTGAAGGAAGGGTAGCAT+CGG | - | chr3.4:71818941-71818960 | MS.gene031851:intron | 40.0% |
| !!! | CAGTGTTGGTTTTTGAAGGA+AGG | - | chr3.4:71818931-71818950 | MS.gene031851:intron | 40.0% |
| AAAGAGGTCCCAGTTCAAAG+TGG | + | chr3.4:71819310-71819329 | None:intergenic | 45.0% | |
| ACTTTGAACTGGGACCTCTT+TGG | - | chr3.4:71819309-71819328 | MS.gene031851:intron | 45.0% | |
| AGGATACTGCCACTAGAAGA+AGG | + | chr3.4:71820211-71820230 | None:intergenic | 45.0% | |
| AGTGGAAGTCGAACTACTAG+AGG | - | chr3.4:71819364-71819383 | MS.gene031851:intron | 45.0% | |
| ATTATAGCCGTTCCCCAAAG+AGG | + | chr3.4:71819326-71819345 | None:intergenic | 45.0% | |
| CGGCTATAATGACCTCTTTG+AGG | - | chr3.4:71819336-71819355 | MS.gene031851:intron | 45.0% | |
| CTGTGCAACATGTGCCTAAA+GGG | - | chr3.4:71818970-71818989 | MS.gene031851:intron | 45.0% | |
| CTTTGAACTGGGACCTCTTT+GGG | - | chr3.4:71819310-71819329 | MS.gene031851:intron | 45.0% | |
| GAATTGGGTAGGCAGCTATA+TGG | + | chr3.4:71818912-71818931 | None:intergenic | 45.0% | |
| GACAGTGAGTTAAGACCTCT+GGG | - | chr3.4:71818766-71818785 | MS.gene031851:CDS | 45.0% | |
| GCTTCTAAGCCTTCTTCTAG+TGG | - | chr3.4:71820199-71820218 | MS.gene031851:CDS | 45.0% | |
| GGGGACAAACACAAGTGAAT+AGG | + | chr3.4:71819708-71819727 | None:intergenic | 45.0% | |
| TGACAGTGAGTTAAGACCTC+TGG | - | chr3.4:71818765-71818784 | MS.gene031851:CDS | 45.0% | |
| TGCCAATTTCGGCCAAAACT+AGG | - | chr3.4:71819439-71819458 | MS.gene031851:intron | 45.0% | |
| TTTGAACTGGGACCTCTTTG+GGG | - | chr3.4:71819311-71819330 | MS.gene031851:intron | 45.0% | |
| ! | AAGCACTGTGACTCGACAAA+AGG | - | chr3.4:71819666-71819685 | MS.gene031851:intron | 45.0% |
| ! | AGAGTGTCAGTAACACTTGG+TGG | + | chr3.4:71820062-71820081 | None:intergenic | 45.0% |
| !! | TTGTCCCCTCTGTTCTAAAG+TGG | - | chr3.4:71819720-71819739 | MS.gene031851:intron | 45.0% |
| !!! | TGCCTAGTTTTGGCCGAAAT+TGG | + | chr3.4:71819444-71819463 | None:intergenic | 45.0% |
| !! | GATAAAAAAAATAAAATAAA+TGG | - | chr3.4:71820035-71820054 | MS.gene031851:intron | 5.0% |
| !!! | CTATTTTATATAATTTTTTA+AGG | - | chr3.4:71819615-71819634 | MS.gene031851:intron | 5.0% |
| ACATGTGCCTAAAGGGACTG+AGG | - | chr3.4:71818977-71818996 | MS.gene031851:intron | 50.0% | |
| CTGCCTACCCAATTCAGTGT+TGG | - | chr3.4:71818917-71818936 | MS.gene031851:intron | 50.0% | |
| GCGCATTACATGCAAACTGC+AGG | + | chr3.4:71818735-71818754 | None:intergenic | 50.0% | |
| GCTGTGCAACATGTGCCTAA+AGG | - | chr3.4:71818969-71818988 | MS.gene031851:intron | 50.0% | |
| GGATACAATCCTGTTCCCAG+AGG | + | chr3.4:71818784-71818803 | None:intergenic | 50.0% | |
| ! | GAGTTAAGACCTCTGGGAAC+AGG | - | chr3.4:71818772-71818791 | MS.gene031851:CDS | 50.0% |
| GGTTTCTTGCTCCGCACAAC+TGG | - | chr3.4:71819923-71819942 | MS.gene031851:intron | 55.0% | |
| ! | ACTGGGACCTCTTTGGGGAA+CGG | - | chr3.4:71819316-71819335 | MS.gene031851:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 71818715 | 71820234 | 71818715 | ID=MS.gene031851 |
| chr3.4 | mRNA | 71818715 | 71820234 | 71818715 | ID=MS.gene031851.t1;Parent=MS.gene031851 |
| chr3.4 | exon | 71820122 | 71820234 | 71820122 | ID=MS.gene031851.t1.exon1;Parent=MS.gene031851.t1 |
| chr3.4 | CDS | 71820122 | 71820234 | 71820122 | ID=cds.MS.gene031851.t1;Parent=MS.gene031851.t1 |
| chr3.4 | exon | 71819951 | 71820032 | 71819951 | ID=MS.gene031851.t1.exon2;Parent=MS.gene031851.t1 |
| chr3.4 | CDS | 71819951 | 71820032 | 71819951 | ID=cds.MS.gene031851.t1;Parent=MS.gene031851.t1 |
| chr3.4 | exon | 71819208 | 71819312 | 71819208 | ID=MS.gene031851.t1.exon3;Parent=MS.gene031851.t1 |
| chr3.4 | CDS | 71819208 | 71819312 | 71819208 | ID=cds.MS.gene031851.t1;Parent=MS.gene031851.t1 |
| chr3.4 | exon | 71819005 | 71819095 | 71819005 | ID=MS.gene031851.t1.exon4;Parent=MS.gene031851.t1 |
| chr3.4 | CDS | 71819005 | 71819095 | 71819005 | ID=cds.MS.gene031851.t1;Parent=MS.gene031851.t1 |
| chr3.4 | exon | 71818715 | 71818845 | 71818715 | ID=MS.gene031851.t1.exon5;Parent=MS.gene031851.t1 |
| chr3.4 | CDS | 71818715 | 71818845 | 71818715 | ID=cds.MS.gene031851.t1;Parent=MS.gene031851.t1 |
| Gene Sequence |
| Protein sequence |
