AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031868

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031868.t1 MTR_3g072710 97.441 508 9 2 1 504 1 508 0.0 1017
MS.gene031868.t1 MTR_5g058860 63.022 503 148 14 12 499 21 500 0.0 578
MS.gene031868.t1 MTR_8g093070 38.974 390 200 12 116 496 136 496 2.11e-67 225
MS.gene031868.t1 MTR_7g027190 33.607 488 273 15 30 497 218 674 2.89e-65 223
MS.gene031868.t1 MTR_3g065980 31.195 452 255 13 76 502 126 546 1.12e-52 187
MS.gene031868.t1 MTR_7g074650 37.203 379 201 16 124 495 443 791 1.42e-51 188
MS.gene031868.t1 MTR_1g069725 31.100 418 253 12 89 500 79 467 8.62e-49 175
MS.gene031868.t1 MTR_4g076020 36.828 372 193 13 133 498 96 431 2.45e-48 173
MS.gene031868.t1 MTR_3g089055 31.931 404 239 12 104 495 190 569 3.29e-48 175
MS.gene031868.t1 MTR_3g089055 31.931 404 239 12 104 495 185 564 4.56e-48 175
MS.gene031868.t1 MTR_1g029420 32.648 389 230 11 118 495 224 591 8.37e-48 174
MS.gene031868.t1 MTR_4g104020 31.683 404 252 9 103 497 133 521 2.87e-47 172
MS.gene031868.t1 MTR_8g442410 31.674 442 260 13 81 499 106 528 6.66e-45 166
MS.gene031868.t1 MTR_4g077760 32.609 368 229 8 131 495 203 554 9.66e-45 165
MS.gene031868.t1 MTR_5g097480 29.397 398 251 10 107 495 167 543 5.88e-43 160
MS.gene031868.t1 MTR_2g082090 31.961 413 240 15 101 495 189 578 9.54e-43 160
MS.gene031868.t1 MTR_1g096030 31.442 423 220 16 109 495 70 458 8.33e-40 150
MS.gene031868.t1 MTR_3g056110 30.623 369 222 10 140 495 194 541 1.35e-39 151
MS.gene031868.t1 MTR_4g102790 30.993 413 222 16 112 496 285 662 2.77e-39 151
MS.gene031868.t1 MTR_5g094450 28.424 387 257 8 116 495 158 531 7.36e-39 149
MS.gene031868.t1 MTR_4g133660 28.244 393 253 8 114 497 172 544 1.24e-37 145
MS.gene031868.t1 MTR_2g026250 27.171 357 228 11 154 497 260 597 3.54e-35 139
MS.gene031868.t1 MTR_2g034250 26.835 395 271 9 109 495 200 584 1.21e-34 137
MS.gene031868.t1 MTR_2g034260 27.681 401 259 14 109 495 200 583 2.50e-33 133
MS.gene031868.t1 MTR_4g064150 25.238 420 282 11 89 496 334 733 3.45e-32 131
MS.gene031868.t1 MTR_2g034280 26.531 392 273 10 109 495 193 574 3.60e-32 130
MS.gene031868.t1 MTR_4g064200 28.362 409 240 17 111 498 276 652 1.80e-31 128
MS.gene031868.t1 MTR_2g097410 26.329 414 258 14 103 496 355 741 2.18e-31 129
MS.gene031868.t1 MTR_7g062120 27.143 420 263 12 93 495 346 739 8.48e-31 127
MS.gene031868.t1 MTR_2g097467 28.032 371 225 14 144 496 308 654 1.51e-29 123
MS.gene031868.t1 MTR_4g097080 27.461 386 257 11 118 495 132 502 3.24e-29 120
MS.gene031868.t1 MTR_2g099110 29.595 321 196 10 98 409 53 352 3.78e-29 120
MS.gene031868.t1 MTR_4g064120 24.311 399 269 11 66 449 201 581 1.53e-28 119
MS.gene031868.t1 MTR_3g022830 24.554 448 258 13 105 495 11 435 5.96e-28 116
MS.gene031868.t1 MTR_0092s0100 28.205 390 243 15 116 497 372 732 5.97e-28 118
MS.gene031868.t1 MTR_0092s0100 28.205 390 243 15 116 497 369 729 6.62e-28 118
MS.gene031868.t1 MTR_2g097463 29.936 314 189 12 200 496 356 655 4.53e-27 115
MS.gene031868.t1 MTR_4g064160 25.175 429 280 13 84 496 281 684 4.68e-27 115
MS.gene031868.t1 MTR_5g015490 27.500 400 245 14 118 495 113 489 1.46e-26 112
MS.gene031868.t1 MTR_4g064180 24.590 427 284 13 87 497 220 624 1.07e-25 111
MS.gene031868.t1 MTR_2g097473 27.933 358 220 13 154 496 320 654 2.06e-25 110
MS.gene031868.t1 MTR_7g109580 25.377 398 263 14 109 495 179 553 3.60e-25 109
MS.gene031868.t1 MTR_2g097390 24.831 443 280 15 71 495 233 640 1.81e-24 107
MS.gene031868.t1 MTR_2g097310 24.605 443 283 15 71 496 230 638 1.45e-23 104
MS.gene031868.t1 MTR_8g077940 28.267 375 227 13 136 497 197 542 3.58e-23 103
MS.gene031868.t1 MTR_4g026485 26.379 417 266 15 86 497 245 625 4.06e-22 100
MS.gene031868.t1 MTR_2g097350 25.823 395 246 15 119 495 274 639 5.86e-22 99.8
MS.gene031868.t1 MTR_2g089100 28.037 321 191 10 210 498 133 445 6.31e-22 99.0
MS.gene031868.t1 MTR_4g076140 30.043 233 142 7 105 335 34 247 2.18e-21 97.4
MS.gene031868.t1 MTR_4g076140 28.000 150 102 3 349 495 322 468 2.44e-12 69.3
MS.gene031868.t1 MTR_5g009080 28.936 235 144 8 104 335 41 255 5.36e-21 96.3
MS.gene031868.t1 MTR_5g009080 31.646 158 94 5 345 495 327 477 3.48e-13 72.0
MS.gene031868.t1 MTR_2g097380 24.528 371 202 14 148 496 247 561 1.52e-20 95.1
MS.gene031868.t1 MTR_7g069740 26.161 409 253 14 105 495 206 583 3.07e-20 94.4
MS.gene031868.t1 MTR_1g086970 23.500 400 275 12 109 495 96 477 2.61e-19 90.9
MS.gene031868.t1 MTR_8g020840 24.495 396 263 12 118 495 170 547 2.29e-14 75.9
MS.gene031868.t1 MTR_4g095500 23.488 430 257 16 118 495 58 467 7.49e-13 70.9
MS.gene031868.t1 MTR_5g015950 21.564 422 277 10 118 495 34 445 9.21e-13 70.5
MS.gene031868.t1 MTR_3g053270 21.564 422 277 10 118 495 34 445 9.21e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031868.t1 AT4G08250 50.898 501 208 12 11 501 10 482 1.29e-158 459
MS.gene031868.t1 AT3G54220 36.386 415 229 16 94 499 265 653 6.11e-52 187
MS.gene031868.t1 AT5G41920 34.739 403 236 12 102 501 27 405 4.97e-51 179
MS.gene031868.t1 AT1G66350 32.609 414 232 15 90 495 131 505 4.99e-48 174
MS.gene031868.t1 AT3G03450 31.514 403 233 14 107 495 171 544 4.00e-46 169
MS.gene031868.t1 AT5G66770 32.029 409 255 7 93 495 192 583 4.40e-46 169
MS.gene031868.t1 AT5G17490 32.446 413 237 14 98 499 138 519 1.34e-45 167
MS.gene031868.t1 AT3G50650 33.425 365 215 7 141 495 195 541 3.33e-44 164
MS.gene031868.t1 AT1G55580 34.505 313 188 7 196 495 136 444 7.89e-44 160
MS.gene031868.t1 AT5G48150 29.897 388 239 10 120 495 123 489 9.37e-43 159
MS.gene031868.t1 AT5G48150 29.897 388 239 10 120 495 123 489 9.37e-43 159
MS.gene031868.t1 AT5G48150 29.897 388 239 10 120 495 123 489 9.37e-43 159
MS.gene031868.t1 AT5G48150 29.897 388 239 10 120 495 123 489 9.37e-43 159
MS.gene031868.t1 AT2G01570 31.204 407 234 14 107 500 212 585 1.71e-41 157
MS.gene031868.t1 AT4G17230 31.608 367 213 10 144 495 181 524 1.13e-40 154
MS.gene031868.t1 AT4G17230 31.608 367 213 10 144 495 181 524 1.13e-40 154
MS.gene031868.t1 AT1G21450 31.202 391 234 14 118 495 224 592 3.31e-40 153
MS.gene031868.t1 AT1G14920 29.756 410 236 16 107 500 160 533 6.29e-39 149
MS.gene031868.t1 AT2G04890 31.156 398 230 14 111 495 46 412 6.64e-39 146
MS.gene031868.t1 AT1G50600 30.789 380 229 12 130 495 166 525 5.59e-38 146
MS.gene031868.t1 AT1G50600 30.789 380 229 12 130 495 237 596 1.33e-37 146
MS.gene031868.t1 AT1G50600 30.789 380 229 12 130 495 241 600 1.35e-37 146
MS.gene031868.t1 AT1G07530 27.938 451 281 13 65 500 348 769 3.98e-37 146
MS.gene031868.t1 AT1G63100 29.314 423 261 13 93 496 226 629 7.80e-37 144
MS.gene031868.t1 AT1G63100 29.314 423 261 13 93 496 251 654 9.98e-37 144
MS.gene031868.t1 AT2G29065 26.598 391 260 10 119 496 252 628 4.14e-32 130
MS.gene031868.t1 AT2G29060 25.745 470 290 17 51 496 221 655 1.40e-31 129
MS.gene031868.t1 AT2G29060 25.745 470 290 17 51 496 258 692 1.61e-31 129
MS.gene031868.t1 AT1G07520 28.022 364 226 10 154 498 349 695 4.60e-31 127
MS.gene031868.t1 AT1G07520 27.222 360 234 9 154 498 350 696 4.70e-31 127
MS.gene031868.t1 AT1G07520 28.022 364 226 10 154 498 185 531 5.36e-31 126
MS.gene031868.t1 AT1G07520 28.022 364 226 10 154 498 185 531 5.36e-31 126
MS.gene031868.t1 AT3G46600 25.778 450 284 12 66 495 159 578 8.28e-31 126
MS.gene031868.t1 AT3G46600 27.638 398 238 12 118 495 3 370 9.91e-31 123
MS.gene031868.t1 AT3G46600 25.778 450 284 12 66 495 127 546 1.52e-30 125
MS.gene031868.t1 AT4G37650 27.820 399 250 13 119 495 146 528 7.41e-30 123
MS.gene031868.t1 AT5G59450 25.000 452 293 12 68 495 168 597 2.39e-29 122
MS.gene031868.t1 AT3G60630 27.970 404 257 12 110 497 238 623 1.67e-28 119
MS.gene031868.t1 AT2G37650 28.321 399 251 13 114 500 342 717 9.08e-27 114
MS.gene031868.t1 AT2G37650 28.321 399 251 13 114 500 330 705 9.22e-27 114
MS.gene031868.t1 AT2G45160 27.251 411 246 14 108 497 262 640 5.22e-26 112
MS.gene031868.t1 AT4G36710 27.913 369 233 12 136 495 140 484 6.03e-26 111
MS.gene031868.t1 AT4G00150 27.436 390 234 13 120 500 209 558 5.28e-21 96.7
MS.gene031868.t1 AT1G50420 32.203 236 135 9 105 335 43 258 2.58e-20 94.4
MS.gene031868.t1 AT1G50420 34.641 153 94 3 348 497 331 480 1.14e-17 86.3
MS.gene031868.t1 AT3G49950 25.469 373 225 13 153 496 60 408 2.78e-19 90.5
MS.gene031868.t1 AT5G52510 28.793 323 188 10 202 495 330 639 8.20e-18 87.0

Find 192 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGGTGAGCTAGGGTTTA+GGG 0.226810 3.3:-63029612 MS.gene031868:CDS
CCTACTTCCGACGATGATTA+TGG 0.250428 3.3:-63030856 MS.gene031868:CDS
GGTTAGGTGAGCTAGGGTTT+AGG 0.289634 3.3:-63029613 MS.gene031868:CDS
GTCAACTGGGACACCTTTAC+AGG 0.297676 3.3:-63030805 MS.gene031868:CDS
GCAGAACCGGGCCCGGGCTT+TGG 0.319278 3.3:-63029729 MS.gene031868:CDS
GGAGAGTGGTTAGGTGAGCT+AGG 0.324695 3.3:-63029620 MS.gene031868:CDS
CATTTCACCGAAGCCCTTCA+TGG 0.325802 3.3:-63030439 MS.gene031868:CDS
TGTCAAGCAAAGCTGTTGTT+AGG 0.326046 3.3:-63029563 MS.gene031868:CDS
TGGGTTTGTGGAAAGGTTCA+TGG 0.326737 3.3:-63029807 MS.gene031868:CDS
TCCACCAACGTCACAAGCTT+TGG 0.330745 3.3:+63029854 None:intergenic
ACTGGTCTCCCATCGTCAAC+TGG 0.335690 3.3:-63030819 MS.gene031868:CDS
AGCTTCGATGATGGCTTGAT+TGG 0.338111 3.3:+63030261 None:intergenic
GGTGGTGGTGGTTGTGGTGA+TGG 0.340117 3.3:+63030705 None:intergenic
CTGGGTTTCCGATGCAGAAC+CGG 0.348964 3.3:-63029742 MS.gene031868:CDS
GTGGTGGTTGTGGTGATGGA+TGG 0.363760 3.3:+63030709 None:intergenic
ATCAATGACCTCCACTTCTC+TGG 0.368955 3.3:-63030901 MS.gene031868:CDS
ACCTCCTTCGCCGCTTCCCT+TGG 0.370284 3.3:-63030055 MS.gene031868:CDS
CCTAGGATTGCAGGCTCGTT+GGG 0.371680 3.3:-63029686 MS.gene031868:CDS
GGTTGTGGTGGTGGTGGTTG+TGG 0.377950 3.3:+63030699 None:intergenic
TTCGGTAGTTGGTGGGTTTG+TGG 0.382194 3.3:-63029819 MS.gene031868:CDS
GCTTGTACGTGGAGAGGCTT+TGG 0.384610 3.3:-63029966 MS.gene031868:CDS
CTTCCGCGCCAGCCATCAAT+AGG 0.391873 3.3:+63030572 None:intergenic
GTTTGCTTTCTGCTTCCCTT+TGG 0.394498 3.3:-63029463 MS.gene031868:CDS
TTCCGATGCAGAACCGGGCC+CGG 0.397897 3.3:-63029736 MS.gene031868:CDS
ATGAGGTGGTGAAAGTCATC+TGG 0.398824 3.3:+63030778 None:intergenic
GCCTAGGATTGCAGGCTCGT+TGG 0.400453 3.3:-63029687 MS.gene031868:CDS
GAAGAAGTTGGTTCGGTAGT+TGG 0.401602 3.3:-63029830 MS.gene031868:CDS
TTCTTCCTCATCGGTGGTTG+TGG 0.404871 3.3:+63030684 None:intergenic
TAGTTACCGTGCACCGGAAT+CGG 0.409078 3.3:-63029912 MS.gene031868:CDS
CGATGAGGAAGAAGAAGAAA+TGG 0.410332 3.3:-63030674 MS.gene031868:CDS
AAGCAGCAAGTGTGTCGTTT+TGG 0.410870 3.3:+63030335 None:intergenic
CATTTCTTCTTCTTCCTCAT+CGG 0.412349 3.3:+63030675 None:intergenic
TCCGATGCAGAACCGGGCCC+GGG 0.417300 3.3:-63029735 MS.gene031868:CDS
GAACCGGGCCCGGGCTTTGG+TGG 0.417856 3.3:-63029726 MS.gene031868:CDS
TGACATGGACGCGGCGTTCG+TGG 0.420981 3.3:+63030233 None:intergenic
AAGAATGAAGCAACCGATTC+CGG 0.425966 3.3:+63029899 None:intergenic
CTCATCGGTGGTTGTGGTGG+TGG 0.431811 3.3:+63030690 None:intergenic
GCATTATCACGGACAGGAAC+CGG 0.434140 3.3:-63030121 MS.gene031868:CDS
CCCAACGAGCCTGCAATCCT+AGG 0.435603 3.3:+63029686 None:intergenic
CATAATCATCGTCGGAAGTA+GGG 0.435805 3.3:+63030857 None:intergenic
TCGCCTGCCGGTTTCTTGTA+CGG 0.437235 3.3:+63030081 None:intergenic
GCTAGGGTTTAGGGGAGTTC+CGG 0.437311 3.3:-63029603 MS.gene031868:CDS
CACCACCCGTTCTATATATC+CGG 0.439796 3.3:+63029663 None:intergenic
GTAATAAGTTGGTGTTGGAT+TGG 0.441173 3.3:-63029496 MS.gene031868:CDS
GCCCTTCATGGACTACTAGA+AGG 0.443992 3.3:-63030427 MS.gene031868:CDS
GAAGTTGGTGATGATTCAAA+AGG 0.445291 3.3:-63030610 MS.gene031868:CDS
ATGTCACCTTATGTCAAATT+CGG 0.447667 3.3:-63030295 MS.gene031868:CDS
AGAGAAGGTCGTGGGGAGAG+TGG 0.453369 3.3:-63029634 MS.gene031868:CDS
GAGAGTGGTTAGGTGAGCTA+GGG 0.454793 3.3:-63029619 MS.gene031868:CDS
TGAGGTAATGTTTGTTGTTG+TGG 0.455641 3.3:+63030380 None:intergenic
AAAACTAGTGCACCTATTGA+TGG 0.457233 3.3:-63030584 MS.gene031868:CDS
GATGGGAGACCAGTGGTTCC+AGG 0.457583 3.3:+63030828 None:intergenic
AGAGAAGTGGAGGTCATTGA+TGG 0.464377 3.3:+63030903 None:intergenic
AAACATTACCTCACCACAAA+TGG 0.466163 3.3:-63030370 MS.gene031868:CDS
TGAAGAGGAGAGAAGGTCGT+GGG 0.469399 3.3:-63029642 MS.gene031868:CDS
GTGGGCCACAGCTTCGATGA+TGG 0.470189 3.3:+63030252 None:intergenic
CCATAATCATCGTCGGAAGT+AGG 0.475182 3.3:+63030856 None:intergenic
AAGATGAGGACCGTTGTTGT+TGG 0.475317 3.3:+63030153 None:intergenic
AGGTCGTGGGGAGAGTGGTT+AGG 0.475697 3.3:-63029629 MS.gene031868:CDS
CATGGACTACTAGAAGGTGC+TGG 0.477897 3.3:-63030421 MS.gene031868:CDS
TCTCCACCAAAGCCCGGGCC+CGG 0.479183 3.3:+63029723 None:intergenic
ACAACAACGGTCCTCATCTT+CGG 0.479785 3.3:-63030150 MS.gene031868:CDS
CGGAAGTAGGGGTGTTGGTG+AGG 0.483268 3.3:+63030869 None:intergenic
GGCCCGGGCTTTGGTGGAGA+GGG 0.485841 3.3:-63029720 MS.gene031868:CDS
ATACCCTCTCCACCAAAGCC+CGG 0.489626 3.3:+63029717 None:intergenic
GTCAAGCAAAGCTGTTGTTA+GGG 0.489648 3.3:-63029562 MS.gene031868:CDS
GCTGGCGCGGAAGCCTTAAC+CGG 0.490918 3.3:-63030562 MS.gene031868:CDS
ATCGCCACCGTACAAGAAAC+CGG 0.491760 3.3:-63030088 MS.gene031868:CDS
TAGGGTTGAAGAGGTGGGTG+TGG 0.493351 3.3:-63029522 MS.gene031868:CDS
TAGTAGTCCATGAAGGGCTT+CGG 0.495192 3.3:+63030432 None:intergenic
TGGGTATAGGGTTGAAGAGG+TGG 0.495501 3.3:-63029528 MS.gene031868:CDS
ATGGTTGCACCTGGAACCAC+TGG 0.499209 3.3:-63030837 MS.gene031868:CDS
ATCGGTGGTTGTGGTGGTGG+TGG 0.499565 3.3:+63030693 None:intergenic
TAGTTGGTGGGTTTGTGGAA+AGG 0.499576 3.3:-63029814 MS.gene031868:CDS
ATTCCGGTGCACGGTAACTA+AGG 0.501357 3.3:+63029915 None:intergenic
TTCTGTCGTTGTTGTTGTTG+TGG 0.501931 3.3:+63030642 None:intergenic
GTCATCTGGGGCACCTGTAA+AGG 0.502615 3.3:+63030792 None:intergenic
TTGTCCAAGGGAAGCGGCGA+AGG 0.503279 3.3:+63030051 None:intergenic
GACATGGACGCGGCGTTCGT+GGG 0.503853 3.3:+63030234 None:intergenic
GTGACATGTCTTGAAGTAGT+TGG 0.504360 3.3:+63030311 None:intergenic
GGAAGTAGGGGTGTTGGTGA+GGG 0.508441 3.3:+63030870 None:intergenic
TGGGTTTCCGATGCAGAACC+GGG 0.509255 3.3:-63029741 MS.gene031868:CDS
CATCATGGAAGGGGTCCAAT+GGG 0.510759 3.3:-63030200 MS.gene031868:CDS
CACCTTCTAGTAGTCCATGA+AGG 0.513198 3.3:+63030425 None:intergenic
GGGTATAGGGTTGAAGAGGT+GGG 0.515764 3.3:-63029527 MS.gene031868:CDS
TTCTTCTTCTTCCTCATCGG+TGG 0.515776 3.3:+63030678 None:intergenic
TGTTGTTGGAAGCTAGTGAT+TGG 0.517924 3.3:+63030167 None:intergenic
GGATTGCAGGCTCGTTGGGC+CGG 0.518025 3.3:-63029682 MS.gene031868:CDS
GGGCCCGGGCTTTGGTGGAG+AGG 0.520373 3.3:-63029721 MS.gene031868:CDS
ATGGACTACTAGAAGGTGCT+GGG 0.522101 3.3:-63030420 MS.gene031868:CDS
GTGAAGAGGAGAGAAGGTCG+TGG 0.522620 3.3:-63029643 MS.gene031868:CDS
GTACGGTGGCGATTGACCTC+CGG 0.522939 3.3:+63030098 None:intergenic
TACCCTCTCCACCAAAGCCC+GGG 0.525259 3.3:+63029718 None:intergenic
ATATAGAACGGGTGGTGAAG+AGG 0.526637 3.3:-63029657 MS.gene031868:CDS
CTGCAGTGGTGGAAAGAAAA+CGG 0.528193 3.3:+63030028 None:intergenic
ACATCATGGAAGGGGTCCAA+TGG 0.528843 3.3:-63030201 MS.gene031868:CDS
CTAGTGCACCTATTGATGGC+TGG 0.529907 3.3:-63030580 MS.gene031868:CDS
GGGAGCTGTGTCCAGAGAAG+TGG 0.530921 3.3:+63030890 None:intergenic
GTTGTCATGGGGACCATTTG+TGG 0.530976 3.3:+63030357 None:intergenic
GGGTAGTAATAAGTTGGTGT+TGG 0.531656 3.3:-63029501 MS.gene031868:CDS
CAGTAATACCATGGACATGA+TGG 0.531857 3.3:-63030935 None:intergenic
AAGTTGGTTCGGTAGTTGGT+GGG 0.534092 3.3:-63029826 MS.gene031868:CDS
GGTGGAAGAAGAAGTTGGTT+CGG 0.535127 3.3:-63029837 MS.gene031868:CDS
TGAAGAAGAACAAGTCCAAA+GGG 0.536908 3.3:+63029448 None:intergenic
GAAGTTGGTTCGGTAGTTGG+TGG 0.538694 3.3:-63029827 MS.gene031868:CDS
CTGGTCTCCCATCGTCAACT+GGG 0.539421 3.3:-63030818 MS.gene031868:CDS
TTAGGTGAGCTAGGGTTTAG+GGG 0.540463 3.3:-63029611 MS.gene031868:CDS
CCTGCCGGTTTCTTGTACGG+TGG 0.541192 3.3:+63030084 None:intergenic
ACATGCTAACGGCTCTAACA+TGG 0.542095 3.3:-63030476 MS.gene031868:CDS
CAATGATTTGCAAAGCTCAC+CGG 0.544682 3.3:+63029584 None:intergenic
GCCCGGGCCCGGTTCTGCAT+CGG 0.547611 3.3:+63029734 None:intergenic
GGGTGTGGGTAGTAATAAGT+TGG 0.548725 3.3:-63029507 MS.gene031868:CDS
TGATGGGTATAGGGTTGAAG+AGG 0.549968 3.3:-63029531 MS.gene031868:CDS
GTGAGACTAATTCCTTGAGC+CGG 0.552046 3.3:+63030500 None:intergenic
CGGGTGGTGAAGAGGAGAGA+AGG 0.553224 3.3:-63029649 MS.gene031868:CDS
TGTCATCGACTACGACATCA+TGG 0.554847 3.3:-63030215 MS.gene031868:CDS
TGAGGTGGTGAAAGTCATCT+GGG 0.555233 3.3:+63030779 None:intergenic
TAGCTCGAGTGATATTGATC+CGG 0.555972 3.3:-63030519 MS.gene031868:CDS
AGGGTTGAAGAGGTGGGTGT+GGG 0.556341 3.3:-63029521 MS.gene031868:CDS
TCGGTTGCTTCATTCTTGAA+CGG 0.556699 3.3:-63029893 MS.gene031868:CDS
TTCCTCATCGGTGGTTGTGG+TGG 0.557874 3.3:+63030687 None:intergenic
ATCGTCGGAAGTAGGGGTGT+TGG 0.558472 3.3:+63030864 None:intergenic
ATCCATGTCCATCATGTCCA+TGG 0.558666 3.3:+63030927 None:intergenic
GGAAAGAAAACGGTTGTCCA+AGG 0.560068 3.3:+63030038 None:intergenic
GAAGAGGAGAGAAGGTCGTG+GGG 0.560976 3.3:-63029641 MS.gene031868:CDS
TTCGGATAACCGCATTATCA+CGG 0.561276 3.3:-63030132 MS.gene031868:CDS
GGTAATGTTTGTTGTTGTGG+TGG 0.561567 3.3:+63030383 None:intergenic
AGATGACTTTCACCACCTCA+TGG 0.561702 3.3:-63030776 MS.gene031868:CDS
CTGAGTCTAACCTGCAGTGG+TGG 0.569298 3.3:+63030017 None:intergenic
TTGGGCCGGATATATAGAAC+GGG 0.569905 3.3:-63029668 MS.gene031868:CDS
AAGTGGCCGAATTTGACATA+AGG 0.570280 3.3:+63030289 None:intergenic
ATTGGATTAATGAAGCCCAT+TGG 0.571351 3.3:+63030185 None:intergenic
AAACGGTTGTCCAAGGGAAG+CGG 0.571859 3.3:+63030045 None:intergenic
TCACCTTAGTTACCGTGCAC+CGG 0.573305 3.3:-63029918 MS.gene031868:CDS
ATCGACTACGACATCATGGA+AGG 0.578928 3.3:-63030211 MS.gene031868:CDS
CCACCGTACAAGAAACCGGC+AGG 0.581224 3.3:-63030084 MS.gene031868:CDS
TCCAAGGGAAGCGGCGAAGG+AGG 0.582127 3.3:+63030054 None:intergenic
ACGATGATTATGGTTGCACC+TGG 0.582304 3.3:-63030846 MS.gene031868:CDS
AGTGATATTGATCCGGCTCA+AGG 0.583439 3.3:-63030512 MS.gene031868:CDS
CTGAAGAAGAACAAGTCCAA+AGG 0.584472 3.3:+63029447 None:intergenic
AGCTGTGTCCAGAGAAGTGG+AGG 0.585513 3.3:+63030893 None:intergenic
AAGGTGTCCCAGTTGACGAT+GGG 0.586507 3.3:+63030811 None:intergenic
CATCTGAGTCTAACCTGCAG+TGG 0.589878 3.3:+63030014 None:intergenic
AAGTTGGTGATGATTCAAAA+GGG 0.593257 3.3:-63030609 MS.gene031868:CDS
GAAGGAGGTTAATCGCCTGC+CGG 0.597149 3.3:+63030069 None:intergenic
CGACTACGACATCATGGAAG+GGG 0.598773 3.3:-63030209 MS.gene031868:CDS
ACCTTCTAGTAGTCCATGAA+GGG 0.600557 3.3:+63030426 None:intergenic
CTCACTTCATCATTATTCAG+CGG 0.602060 3.3:-63029783 MS.gene031868:CDS
TCCAAAGCTTGTGACGTTGG+TGG 0.608726 3.3:-63029855 MS.gene031868:CDS
GCACCTATTGATGGCTGGCG+CGG 0.609141 3.3:-63030575 MS.gene031868:CDS
ATTAAAGCTTGTACGTGGAG+AGG 0.611742 3.3:-63029972 MS.gene031868:CDS
ATAATCATCGTCGGAAGTAG+GGG 0.612654 3.3:+63030858 None:intergenic
TCAAGCCATCATCGAAGCTG+TGG 0.613643 3.3:-63030257 MS.gene031868:CDS
AAGCAACCGATTCCGGTGCA+CGG 0.622561 3.3:+63029906 None:intergenic
CGTACAAGCTTTAATGCAGA+AGG 0.623940 3.3:+63029980 None:intergenic
TTAGTCTCACAACATGCTAA+CGG 0.625561 3.3:-63030487 MS.gene031868:CDS
CACGGACAGGAACCGGCCGG+AGG 0.629234 3.3:-63030114 MS.gene031868:CDS
AATGCGGTTATCCGAAGATG+AGG 0.632509 3.3:+63030139 None:intergenic
AAAGGTGTCCCAGTTGACGA+TGG 0.634828 3.3:+63030810 None:intergenic
TGGATGGACTAAGCTGGTCG+AGG 0.635528 3.3:+63030725 None:intergenic
GTTGGGCCGGATATATAGAA+CGG 0.635781 3.3:-63029669 MS.gene031868:CDS
GGTTCCTGTCCGTGATAATG+CGG 0.642931 3.3:+63030123 None:intergenic
CGATGATTGTGTCCATGAGG+TGG 0.643118 3.3:+63030764 None:intergenic
TCGACTACGACATCATGGAA+GGG 0.644449 3.3:-63030210 MS.gene031868:CDS
GAATCCAAAGCTTGTGACGT+TGG 0.644734 3.3:-63029858 MS.gene031868:CDS
CACCACCACAACCACCGATG+AGG 0.645241 3.3:-63030689 MS.gene031868:CDS
ATAACCGCATTATCACGGAC+AGG 0.646058 3.3:-63030127 MS.gene031868:CDS
TATCACGGACAGGAACCGGC+CGG 0.647005 3.3:-63030117 MS.gene031868:CDS
TGATGTCGTAGTCGATGACA+TGG 0.647412 3.3:+63030218 None:intergenic
ATATCACTCGAGCTAAGTCA+CGG 0.648503 3.3:+63030527 None:intergenic
TGGCTTGATTGGCTGTGAAG+TGG 0.648617 3.3:+63030272 None:intergenic
ACTAAGCTGGTCGAGGACAG+TGG 0.650840 3.3:+63030732 None:intergenic
GAAAGAAAACGGTTGTCCAA+GGG 0.651213 3.3:+63030039 None:intergenic
TGGACTACTAGAAGGTGCTG+GGG 0.652604 3.3:-63030419 MS.gene031868:CDS
CATGGGGACCATTTGTGGTG+AGG 0.653840 3.3:+63030362 None:intergenic
AAGGGCTTCGGTGAAATGAG+CGG 0.654823 3.3:+63030444 None:intergenic
TCTGCATTAAAGCTTGTACG+TGG 0.657776 3.3:-63029977 MS.gene031868:CDS
CGTAGTCGATGACATGGACG+CGG 0.658711 3.3:+63030224 None:intergenic
TACCATGGACATGATGGACA+TGG 0.658713 3.3:-63030929 None:intergenic
TGGTGATGGATGGACTAAGC+TGG 0.659130 3.3:+63030719 None:intergenic
GGCCGGATATATAGAACGGG+TGG 0.661675 3.3:-63029665 MS.gene031868:CDS
GGTGCAACCATAATCATCGT+CGG 0.661939 3.3:+63030849 None:intergenic
CAAGCTTTAATGCAGAAGGA+CGG 0.671677 3.3:+63029984 None:intergenic
GGACTACTAGAAGGTGCTGG+GGG 0.674008 3.3:-63030418 MS.gene031868:CDS
GGTGGCGATTGACCTCCGGC+CGG 0.683391 3.3:+63030102 None:intergenic
AGTTGACGATGGGAGACCAG+TGG 0.695859 3.3:+63030821 None:intergenic
ACGTTGGTGGAAGAAGAAGT+TGG 0.697366 3.3:-63029842 MS.gene031868:CDS
GGTGCACGGTAACTAAGGTG+AGG 0.709219 3.3:+63029920 None:intergenic
TCACTAGCTTCCAACAACAA+CGG 0.720928 3.3:-63030163 MS.gene031868:CDS
GAGGTGGTGAAAGTCATCTG+GGG 0.725269 3.3:+63030780 None:intergenic
CCTCGATGATTGTGTCCATG+AGG 0.737785 3.3:+63030761 None:intergenic
CCTCATGGACACAATCATCG+AGG 0.768679 3.3:-63030761 MS.gene031868:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAGAAAAAACACAAGAAGT+TGG - chr3.3:63029733-63029752 MS.gene031868:CDS 30.0%
ATGTCACCTTATGTCAAATT+CGG - chr3.3:63030063-63030082 MS.gene031868:CDS 30.0%
! TGTTAGGGCTTTTTAATGAT+GGG - chr3.3:63030811-63030830 MS.gene031868:CDS 30.0%
! TTGTTAGGGCTTTTTAATGA+TGG - chr3.3:63030810-63030829 MS.gene031868:CDS 30.0%
!! AAGTTGGTGATGATTCAAAA+GGG - chr3.3:63029749-63029768 MS.gene031868:CDS 30.0%
!!! GCTTTTTAATGATGGGTATA+GGG - chr3.3:63030818-63030837 MS.gene031868:CDS 30.0%
AAAACTAGTGCACCTATTGA+TGG - chr3.3:63029774-63029793 MS.gene031868:CDS 35.0%
AAACATTACCTCACCACAAA+TGG - chr3.3:63029988-63030007 MS.gene031868:CDS 35.0%
ATTGGATTAATGAAGCCCAT+TGG + chr3.3:63030176-63030195 None:intergenic 35.0%
CATTTCTTCTTCTTCCTCAT+CGG + chr3.3:63029686-63029705 None:intergenic 35.0%
CTCACTTCATCATTATTCAG+CGG - chr3.3:63030575-63030594 MS.gene031868:CDS 35.0%
TGAAGAAGAACAAGTCCAAA+GGG + chr3.3:63030913-63030932 None:intergenic 35.0%
TTAGTCTCACAACATGCTAA+CGG - chr3.3:63029871-63029890 MS.gene031868:CDS 35.0%
!! GAAGTTGGTGATGATTCAAA+AGG - chr3.3:63029748-63029767 MS.gene031868:CDS 35.0%
!! GTAATAAGTTGGTGTTGGAT+TGG - chr3.3:63030862-63030881 MS.gene031868:CDS 35.0%
!! TGAGGTAATGTTTGTTGTTG+TGG + chr3.3:63029981-63030000 None:intergenic 35.0%
!! TTCAGCGGTTTTTGATTCAT+TGG - chr3.3:63030590-63030609 MS.gene031868:CDS 35.0%
!! TTTTTGATTCATTGGAGGCT+GGG - chr3.3:63030598-63030617 MS.gene031868:CDS 35.0%
!!! GGCTTTTTAATGATGGGTAT+AGG - chr3.3:63030817-63030836 MS.gene031868:CDS 35.0%
AAGTGGCCGAATTTGACATA+AGG + chr3.3:63030072-63030091 None:intergenic 40.0%
ACCTTCTAGTAGTCCATGAA+GGG + chr3.3:63029935-63029954 None:intergenic 40.0%
ATAATCATCGTCGGAAGTAG+GGG + chr3.3:63029503-63029522 None:intergenic 40.0%
ATATCACTCGAGCTAAGTCA+CGG + chr3.3:63029834-63029853 None:intergenic 40.0%
ATTAAAGCTTGTACGTGGAG+AGG - chr3.3:63030386-63030405 MS.gene031868:CDS 40.0%
CATAATCATCGTCGGAAGTA+GGG + chr3.3:63029504-63029523 None:intergenic 40.0%
CGATGAGGAAGAAGAAGAAA+TGG - chr3.3:63029684-63029703 MS.gene031868:CDS 40.0%
CTGAAGAAGAACAAGTCCAA+AGG + chr3.3:63030914-63030933 None:intergenic 40.0%
GAAAGAAAACGGTTGTCCAA+GGG + chr3.3:63030322-63030341 None:intergenic 40.0%
GTCAAGCAAAGCTGTTGTTA+GGG - chr3.3:63030796-63030815 MS.gene031868:CDS 40.0%
GTGACATGTCTTGAAGTAGT+TGG + chr3.3:63030050-63030069 None:intergenic 40.0%
TAGCTCGAGTGATATTGATC+CGG - chr3.3:63029839-63029858 MS.gene031868:CDS 40.0%
TCGGTTGCTTCATTCTTGAA+CGG - chr3.3:63030465-63030484 MS.gene031868:CDS 40.0%
TCTGCATTAAAGCTTGTACG+TGG - chr3.3:63030381-63030400 MS.gene031868:CDS 40.0%
TGTCAAGCAAAGCTGTTGTT+AGG - chr3.3:63030795-63030814 MS.gene031868:CDS 40.0%
TGTTGTTGGAAGCTAGTGAT+TGG + chr3.3:63030194-63030213 None:intergenic 40.0%
TTCGGATAACCGCATTATCA+CGG - chr3.3:63030226-63030245 MS.gene031868:CDS 40.0%
! AAGAATGAAGCAACCGATTC+CGG + chr3.3:63030462-63030481 None:intergenic 40.0%
! CAAGCTTTAATGCAGAAGGA+CGG + chr3.3:63030377-63030396 None:intergenic 40.0%
! CAATGATTTGCAAAGCTCAC+CGG + chr3.3:63030777-63030796 None:intergenic 40.0%
! CGTACAAGCTTTAATGCAGA+AGG + chr3.3:63030381-63030400 None:intergenic 40.0%
! TCACTAGCTTCCAACAACAA+CGG - chr3.3:63030195-63030214 MS.gene031868:CDS 40.0%
! TTCTGTCGTTGTTGTTGTTG+TGG + chr3.3:63029719-63029738 None:intergenic 40.0%
!! AGCGGTTTTTGATTCATTGG+AGG - chr3.3:63030593-63030612 MS.gene031868:CDS 40.0%
!! GGGTAGTAATAAGTTGGTGT+TGG - chr3.3:63030857-63030876 MS.gene031868:CDS 40.0%
!! GGTAATGTTTGTTGTTGTGG+TGG + chr3.3:63029978-63029997 None:intergenic 40.0%
!! GTTTTTGATTCATTGGAGGC+TGG - chr3.3:63030597-63030616 MS.gene031868:CDS 40.0%
!!! GTTTTTGGTTGAACCGGTTA+AGG + chr3.3:63029812-63029831 None:intergenic 40.0%
!!! TGGTGGAGAGGGTATTTTTT+GGG - chr3.3:63030649-63030668 MS.gene031868:CDS 40.0%
!!! TGTGTCGTTTTGGTTGTCAT+GGG + chr3.3:63030016-63030035 None:intergenic 40.0%
!!! TTGGTGGAGAGGGTATTTTT+TGG - chr3.3:63030648-63030667 MS.gene031868:CDS 40.0%
AAGATGAGGACCGTTGTTGT+TGG + chr3.3:63030208-63030227 None:intergenic 45.0%
AATGCGGTTATCCGAAGATG+AGG + chr3.3:63030222-63030241 None:intergenic 45.0%
ACAACAACGGTCCTCATCTT+CGG - chr3.3:63030208-63030227 MS.gene031868:CDS 45.0%
ACGATGATTATGGTTGCACC+TGG - chr3.3:63029512-63029531 MS.gene031868:CDS 45.0%
AGAGAAGTGGAGGTCATTGA+TGG + chr3.3:63029458-63029477 None:intergenic 45.0%
AGATGACTTTCACCACCTCA+TGG - chr3.3:63029582-63029601 MS.gene031868:CDS 45.0%
AGTGATATTGATCCGGCTCA+AGG - chr3.3:63029846-63029865 MS.gene031868:CDS 45.0%
ATAACCGCATTATCACGGAC+AGG - chr3.3:63030231-63030250 MS.gene031868:CDS 45.0%
ATATAGAACGGGTGGTGAAG+AGG - chr3.3:63030701-63030720 MS.gene031868:CDS 45.0%
ATCAATGACCTCCACTTCTC+TGG - chr3.3:63029457-63029476 MS.gene031868:CDS 45.0%
ATCGACTACGACATCATGGA+AGG - chr3.3:63030147-63030166 MS.gene031868:CDS 45.0%
ATGAGGTGGTGAAAGTCATC+TGG + chr3.3:63029583-63029602 None:intergenic 45.0%
CACCACCCGTTCTATATATC+CGG + chr3.3:63030698-63030717 None:intergenic 45.0%
CACCTTCTAGTAGTCCATGA+AGG + chr3.3:63029936-63029955 None:intergenic 45.0%
CCATAATCATCGTCGGAAGT+AGG + chr3.3:63029505-63029524 None:intergenic 45.0%
CCTACTTCCGACGATGATTA+TGG - chr3.3:63029502-63029521 MS.gene031868:CDS 45.0%
GAATCCAAAGCTTGTGACGT+TGG - chr3.3:63030500-63030519 MS.gene031868:CDS 45.0%
GGAAAGAAAACGGTTGTCCA+AGG + chr3.3:63030323-63030342 None:intergenic 45.0%
GGGTGTGGGTAGTAATAAGT+TGG - chr3.3:63030851-63030870 MS.gene031868:CDS 45.0%
GGTGCAACCATAATCATCGT+CGG + chr3.3:63029512-63029531 None:intergenic 45.0%
GTGAGACTAATTCCTTGAGC+CGG + chr3.3:63029861-63029880 None:intergenic 45.0%
GTTAGGTGAGCTAGGGTTTA+GGG - chr3.3:63030746-63030765 MS.gene031868:CDS 45.0%
GTTGGGCCGGATATATAGAA+CGG - chr3.3:63030689-63030708 MS.gene031868:CDS 45.0%
GTTTGCTTTCTGCTTCCCTT+TGG - chr3.3:63030895-63030914 MS.gene031868:CDS 45.0%
TAGTAGTCCATGAAGGGCTT+CGG + chr3.3:63029929-63029948 None:intergenic 45.0%
TCGACTACGACATCATGGAA+GGG - chr3.3:63030148-63030167 MS.gene031868:CDS 45.0%
TGAGGTGGTGAAAGTCATCT+GGG + chr3.3:63029582-63029601 None:intergenic 45.0%
TGATGTCGTAGTCGATGACA+TGG + chr3.3:63030143-63030162 None:intergenic 45.0%
TGGGTTTGTGGAAAGGTTCA+TGG - chr3.3:63030551-63030570 MS.gene031868:CDS 45.0%
TTAGGTGAGCTAGGGTTTAG+GGG - chr3.3:63030747-63030766 MS.gene031868:CDS 45.0%
TTCTTCTTCTTCCTCATCGG+TGG + chr3.3:63029683-63029702 None:intergenic 45.0%
TTGGGCCGGATATATAGAAC+GGG - chr3.3:63030690-63030709 MS.gene031868:CDS 45.0%
! ACATGCTAACGGCTCTAACA+TGG - chr3.3:63029882-63029901 MS.gene031868:CDS 45.0%
! ACGTTGGTGGAAGAAGAAGT+TGG - chr3.3:63030516-63030535 MS.gene031868:CDS 45.0%
! AGCTTCGATGATGGCTTGAT+TGG + chr3.3:63030100-63030119 None:intergenic 45.0%
! CTGCAGTGGTGGAAAGAAAA+CGG + chr3.3:63030333-63030352 None:intergenic 45.0%
! GGTGGAAGAAGAAGTTGGTT+CGG - chr3.3:63030521-63030540 MS.gene031868:CDS 45.0%
! TCGAGCTAAGTCACGGTTTT+TGG + chr3.3:63029827-63029846 None:intergenic 45.0%
! TGATGGGTATAGGGTTGAAG+AGG - chr3.3:63030827-63030846 MS.gene031868:CDS 45.0%
! TGTCATCGACTACGACATCA+TGG - chr3.3:63030143-63030162 MS.gene031868:CDS 45.0%
!! AAGTTGGTTCGGTAGTTGGT+GGG - chr3.3:63030532-63030551 MS.gene031868:CDS 45.0%
!! ATGGACTACTAGAAGGTGCT+GGG - chr3.3:63029938-63029957 MS.gene031868:CDS 45.0%
!! GAAGAAGTTGGTTCGGTAGT+TGG - chr3.3:63030528-63030547 MS.gene031868:CDS 45.0%
!! TAGTTGGTGGGTTTGTGGAA+AGG - chr3.3:63030544-63030563 MS.gene031868:CDS 45.0%
!!! AAGCAGCAAGTGTGTCGTTT+TGG + chr3.3:63030026-63030045 None:intergenic 45.0%
!!! AGAGGGTATTTTTTGGGCCT+AGG - chr3.3:63030655-63030674 MS.gene031868:CDS 45.0%
!!! GTCACGGTTTTTGGTTGAAC+CGG + chr3.3:63029818-63029837 None:intergenic 45.0%
!!! GTGTCGTTTTGGTTGTCATG+GGG + chr3.3:63030015-63030034 None:intergenic 45.0%
!!! GTGTGTCGTTTTGGTTGTCA+TGG + chr3.3:63030017-63030036 None:intergenic 45.0%
!!! TTTTTTGGGCCTAGGATTGC+AGG - chr3.3:63030663-63030682 MS.gene031868:CDS 45.0%
AAACGGTTGTCCAAGGGAAG+CGG + chr3.3:63030316-63030335 None:intergenic 50.0%
AAGGGCTTCGGTGAAATGAG+CGG + chr3.3:63029917-63029936 None:intergenic 50.0%
ACATCATGGAAGGGGTCCAA+TGG - chr3.3:63030157-63030176 MS.gene031868:CDS 50.0%
ATCGCCACCGTACAAGAAAC+CGG - chr3.3:63030270-63030289 MS.gene031868:CDS 50.0%
ATTCCGGTGCACGGTAACTA+AGG + chr3.3:63030446-63030465 None:intergenic 50.0%
CATCATGGAAGGGGTCCAAT+GGG - chr3.3:63030158-63030177 MS.gene031868:CDS 50.0%
CATCTGAGTCTAACCTGCAG+TGG + chr3.3:63030347-63030366 None:intergenic 50.0%
CATTTCACCGAAGCCCTTCA+TGG - chr3.3:63029919-63029938 MS.gene031868:CDS 50.0%
CCTCATGGACACAATCATCG+AGG - chr3.3:63029597-63029616 MS.gene031868:CDS 50.0%
CCTCGATGATTGTGTCCATG+AGG + chr3.3:63029600-63029619 None:intergenic 50.0%
CGACTACGACATCATGGAAG+GGG - chr3.3:63030149-63030168 MS.gene031868:CDS 50.0%
CGATGATTGTGTCCATGAGG+TGG + chr3.3:63029597-63029616 None:intergenic 50.0%
GAGAGTGGTTAGGTGAGCTA+GGG - chr3.3:63030739-63030758 MS.gene031868:CDS 50.0%
GAGGTGGTGAAAGTCATCTG+GGG + chr3.3:63029581-63029600 None:intergenic 50.0%
GCATTATCACGGACAGGAAC+CGG - chr3.3:63030237-63030256 MS.gene031868:CDS 50.0%
GGGTATAGGGTTGAAGAGGT+GGG - chr3.3:63030831-63030850 MS.gene031868:CDS 50.0%
GGTTAGGTGAGCTAGGGTTT+AGG - chr3.3:63030745-63030764 MS.gene031868:CDS 50.0%
GTCAACTGGGACACCTTTAC+AGG - chr3.3:63029553-63029572 MS.gene031868:CDS 50.0%
GTTGTCATGGGGACCATTTG+TGG + chr3.3:63030004-63030023 None:intergenic 50.0%
TAGTTACCGTGCACCGGAAT+CGG - chr3.3:63030446-63030465 MS.gene031868:CDS 50.0%
TCAAGCCATCATCGAAGCTG+TGG - chr3.3:63030101-63030120 MS.gene031868:CDS 50.0%
TCACCTTAGTTACCGTGCAC+CGG - chr3.3:63030440-63030459 MS.gene031868:CDS 50.0%
TCCAAAGCTTGTGACGTTGG+TGG - chr3.3:63030503-63030522 MS.gene031868:CDS 50.0%
TCCACCAACGTCACAAGCTT+TGG + chr3.3:63030507-63030526 None:intergenic 50.0%
TGAAGAGGAGAGAAGGTCGT+GGG - chr3.3:63030716-63030735 MS.gene031868:CDS 50.0%
TGGGTATAGGGTTGAAGAGG+TGG - chr3.3:63030830-63030849 MS.gene031868:CDS 50.0%
TTCTTCCTCATCGGTGGTTG+TGG + chr3.3:63029677-63029696 None:intergenic 50.0%
! AAGGTGTCCCAGTTGACGAT+GGG + chr3.3:63029550-63029569 None:intergenic 50.0%
! CATGGACTACTAGAAGGTGC+TGG - chr3.3:63029937-63029956 MS.gene031868:CDS 50.0%
! CGTTTTCTTTCCACCACTGC+AGG - chr3.3:63030331-63030350 MS.gene031868:CDS 50.0%
! CTAGTGCACCTATTGATGGC+TGG - chr3.3:63029778-63029797 MS.gene031868:CDS 50.0%
! GCCCTTCATGGACTACTAGA+AGG - chr3.3:63029931-63029950 MS.gene031868:CDS 50.0%
! GGTTCCTGTCCGTGATAATG+CGG + chr3.3:63030238-63030257 None:intergenic 50.0%
! TGGCTTGATTGGCTGTGAAG+TGG + chr3.3:63030089-63030108 None:intergenic 50.0%
!! AAAGGTGTCCCAGTTGACGA+TGG + chr3.3:63029551-63029570 None:intergenic 50.0%
!! GAAGTTGGTTCGGTAGTTGG+TGG - chr3.3:63030531-63030550 MS.gene031868:CDS 50.0%
!! TGGACTACTAGAAGGTGCTG+GGG - chr3.3:63029939-63029958 MS.gene031868:CDS 50.0%
!! TGGTGATGGATGGACTAAGC+TGG + chr3.3:63029642-63029661 None:intergenic 50.0%
!! TTCGGTAGTTGGTGGGTTTG+TGG - chr3.3:63030539-63030558 MS.gene031868:CDS 50.0%
ACTAAGCTGGTCGAGGACAG+TGG + chr3.3:63029629-63029648 None:intergenic 55.0%
AGCTGTGTCCAGAGAAGTGG+AGG + chr3.3:63029468-63029487 None:intergenic 55.0%
AGGGTTGAAGAGGTGGGTGT+GGG - chr3.3:63030837-63030856 MS.gene031868:CDS 55.0%
AGTTGACGATGGGAGACCAG+TGG + chr3.3:63029540-63029559 None:intergenic 55.0%
ATACCCTCTCCACCAAAGCC+CGG + chr3.3:63030644-63030663 None:intergenic 55.0%
ATGGTTGCACCTGGAACCAC+TGG - chr3.3:63029521-63029540 MS.gene031868:CDS 55.0%
CATGGGGACCATTTGTGGTG+AGG + chr3.3:63029999-63030018 None:intergenic 55.0%
CCTAGGATTGCAGGCTCGTT+GGG - chr3.3:63030672-63030691 MS.gene031868:CDS 55.0%
CGTAGTCGATGACATGGACG+CGG + chr3.3:63030137-63030156 None:intergenic 55.0%
CTGAGTCTAACCTGCAGTGG+TGG + chr3.3:63030344-63030363 None:intergenic 55.0%
CTGGGTTTCCGATGCAGAAC+CGG - chr3.3:63030616-63030635 MS.gene031868:CDS 55.0%
CTGGTCTCCCATCGTCAACT+GGG - chr3.3:63029540-63029559 MS.gene031868:CDS 55.0%
GAAGAGGAGAGAAGGTCGTG+GGG - chr3.3:63030717-63030736 MS.gene031868:CDS 55.0%
GAAGGAGGTTAATCGCCTGC+CGG + chr3.3:63030292-63030311 None:intergenic 55.0%
GCTAGGGTTTAGGGGAGTTC+CGG - chr3.3:63030755-63030774 MS.gene031868:CDS 55.0%
GCTTGTACGTGGAGAGGCTT+TGG - chr3.3:63030392-63030411 MS.gene031868:CDS 55.0%
GGAGAGTGGTTAGGTGAGCT+AGG - chr3.3:63030738-63030757 MS.gene031868:CDS 55.0%
GGCCGGATATATAGAACGGG+TGG - chr3.3:63030693-63030712 MS.gene031868:CDS 55.0%
GGTGCACGGTAACTAAGGTG+AGG + chr3.3:63030441-63030460 None:intergenic 55.0%
GTGAAGAGGAGAGAAGGTCG+TGG - chr3.3:63030715-63030734 MS.gene031868:CDS 55.0%
TAGGGTTGAAGAGGTGGGTG+TGG - chr3.3:63030836-63030855 MS.gene031868:CDS 55.0%
TGGGTTTCCGATGCAGAACC+GGG - chr3.3:63030617-63030636 MS.gene031868:CDS 55.0%
TTCCTCATCGGTGGTTGTGG+TGG + chr3.3:63029674-63029693 None:intergenic 55.0%
! AAGCAACCGATTCCGGTGCA+CGG + chr3.3:63030455-63030474 None:intergenic 55.0%
! ACTGGTCTCCCATCGTCAAC+TGG - chr3.3:63029539-63029558 MS.gene031868:CDS 55.0%
! ATCGTCGGAAGTAGGGGTGT+TGG + chr3.3:63029497-63029516 None:intergenic 55.0%
! GTCATCTGGGGCACCTGTAA+AGG + chr3.3:63029569-63029588 None:intergenic 55.0%
!! GGAAGTAGGGGTGTTGGTGA+GGG + chr3.3:63029491-63029510 None:intergenic 55.0%
!! GGACTACTAGAAGGTGCTGG+GGG - chr3.3:63029940-63029959 MS.gene031868:CDS 55.0%
!! GTGGTGGTTGTGGTGATGGA+TGG + chr3.3:63029652-63029671 None:intergenic 55.0%
!! TCGCCTGCCGGTTTCTTGTA+CGG + chr3.3:63030280-63030299 None:intergenic 55.0%
!! TGGATGGACTAAGCTGGTCG+AGG + chr3.3:63029636-63029655 None:intergenic 55.0%
AGAGAAGGTCGTGGGGAGAG+TGG - chr3.3:63030724-63030743 MS.gene031868:CDS 60.0%
AGGTCGTGGGGAGAGTGGTT+AGG - chr3.3:63030729-63030748 MS.gene031868:CDS 60.0%
CACCACCACAACCACCGATG+AGG - chr3.3:63029669-63029688 MS.gene031868:CDS 60.0%
CCACCGTACAAGAAACCGGC+AGG - chr3.3:63030274-63030293 MS.gene031868:CDS 60.0%
CCCAACGAGCCTGCAATCCT+AGG + chr3.3:63030675-63030694 None:intergenic 60.0%
CGGGTGGTGAAGAGGAGAGA+AGG - chr3.3:63030709-63030728 MS.gene031868:CDS 60.0%
CTCATCGGTGGTTGTGGTGG+TGG + chr3.3:63029671-63029690 None:intergenic 60.0%
CTTCCGCGCCAGCCATCAAT+AGG + chr3.3:63029789-63029808 None:intergenic 60.0%
GATGGGAGACCAGTGGTTCC+AGG + chr3.3:63029533-63029552 None:intergenic 60.0%
GCCTAGGATTGCAGGCTCGT+TGG - chr3.3:63030671-63030690 MS.gene031868:CDS 60.0%
GGGAGCTGTGTCCAGAGAAG+TGG + chr3.3:63029471-63029490 None:intergenic 60.0%
GTACGGTGGCGATTGACCTC+CGG + chr3.3:63030263-63030282 None:intergenic 60.0%
GTGGGCCACAGCTTCGATGA+TGG + chr3.3:63030109-63030128 None:intergenic 60.0%
TACCCTCTCCACCAAAGCCC+GGG + chr3.3:63030643-63030662 None:intergenic 60.0%
TATCACGGACAGGAACCGGC+CGG - chr3.3:63030241-63030260 MS.gene031868:CDS 60.0%
TTGTCCAAGGGAAGCGGCGA+AGG + chr3.3:63030310-63030329 None:intergenic 60.0%
! GCACCTATTGATGGCTGGCG+CGG - chr3.3:63029783-63029802 MS.gene031868:CDS 60.0%
!! ATCGGTGGTTGTGGTGGTGG+TGG + chr3.3:63029668-63029687 None:intergenic 60.0%
!! CCTGCCGGTTTCTTGTACGG+TGG + chr3.3:63030277-63030296 None:intergenic 60.0%
!! CGGAAGTAGGGGTGTTGGTG+AGG + chr3.3:63029492-63029511 None:intergenic 60.0%
!! GGTGGTGGTGGTTGTGGTGA+TGG + chr3.3:63029656-63029675 None:intergenic 60.0%
!! GGTTGTGGTGGTGGTGGTTG+TGG + chr3.3:63029662-63029681 None:intergenic 60.0%
ACCTCCTTCGCCGCTTCCCT+TGG - chr3.3:63030303-63030322 MS.gene031868:CDS 65.0%
GACATGGACGCGGCGTTCGT+GGG + chr3.3:63030127-63030146 None:intergenic 65.0%
GCTGGCGCGGAAGCCTTAAC+CGG - chr3.3:63029796-63029815 MS.gene031868:CDS 65.0%
GGATTGCAGGCTCGTTGGGC+CGG - chr3.3:63030676-63030695 MS.gene031868:CDS 65.0%
TCCAAGGGAAGCGGCGAAGG+AGG + chr3.3:63030307-63030326 None:intergenic 65.0%
TGACATGGACGCGGCGTTCG+TGG + chr3.3:63030128-63030147 None:intergenic 65.0%
TTCCGATGCAGAACCGGGCC+CGG - chr3.3:63030622-63030641 MS.gene031868:CDS 65.0%
GGTGGCGATTGACCTCCGGC+CGG + chr3.3:63030259-63030278 None:intergenic 70.0%
TCCGATGCAGAACCGGGCCC+GGG - chr3.3:63030623-63030642 MS.gene031868:CDS 70.0%
TCTCCACCAAAGCCCGGGCC+CGG + chr3.3:63030638-63030657 None:intergenic 70.0%
! GGCCCGGGCTTTGGTGGAGA+GGG - chr3.3:63030638-63030657 MS.gene031868:CDS 70.0%
CACGGACAGGAACCGGCCGG+AGG - chr3.3:63030244-63030263 MS.gene031868:CDS 75.0%
GCAGAACCGGGCCCGGGCTT+TGG - chr3.3:63030629-63030648 MS.gene031868:CDS 75.0%
GCCCGGGCCCGGTTCTGCAT+CGG + chr3.3:63030627-63030646 None:intergenic 75.0%
! GAACCGGGCCCGGGCTTTGG+TGG - chr3.3:63030632-63030651 MS.gene031868:CDS 75.0%
! GGGCCCGGGCTTTGGTGGAG+AGG - chr3.3:63030637-63030656 MS.gene031868:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 63029433 63030947 63029433 ID=MS.gene031868
chr3.3 mRNA 63029433 63030947 63029433 ID=MS.gene031868.t1;Parent=MS.gene031868
chr3.3 exon 63029433 63030947 63029433 ID=MS.gene031868.t1.exon1;Parent=MS.gene031868.t1
chr3.3 CDS 63029433 63030947 63029433 ID=cds.MS.gene031868.t1;Parent=MS.gene031868.t1
Gene Sequence

>MS.gene031868

ATGGACATGATGGACATGGATGCCATCAATGACCTCCACTTCTCTGGACACAGCTCCCTCACCAACACCCCTACTTCCGACGATGATTATGGTTGCACCTGGAACCACTGGTCTCCCATCGTCAACTGGGACACCTTTACAGGTGCCCCAGATGACTTTCACCACCTCATGGACACAATCATCGAGGACAGAACCACTGTCCTCGACCAGCTTAGTCCATCCATCACCACAACCACCACCACCACAACCACCGATGAGGAAGAAGAAGAAATGGAAACAACAACCACAACAACAACAACGACAGAAAAAACACAAGAAGTTGGTGATGATTCAAAAGGGCTAAAACTAGTGCACCTATTGATGGCTGGCGCGGAAGCCTTAACCGGTTCAACCAAAAACCGTGACTTAGCTCGAGTGATATTGATCCGGCTCAAGGAATTAGTCTCACAACATGCTAACGGCTCTAACATGGAGAGACTCGCCGCTCATTTCACCGAAGCCCTTCATGGACTACTAGAAGGTGCTGGGGGTGCGCACAACAACCACCACAACAACAAACATTACCTCACCACAAATGGTCCCCATGACAACCAAAACGACACACTTGCTGCTTTCCAACTACTTCAAGACATGTCACCTTATGTCAAATTCGGCCACTTCACAGCCAATCAAGCCATCATCGAAGCTGTGGCCCACGAACGCCGCGTCCATGTCATCGACTACGACATCATGGAAGGGGTCCAATGGGCTTCATTAATCCAATCACTAGCTTCCAACAACAACGGTCCTCATCTTCGGATAACCGCATTATCACGGACAGGAACCGGCCGGAGGTCAATCGCCACCGTACAAGAAACCGGCAGGCGATTAACCTCCTTCGCCGCTTCCCTTGGACAACCGTTTTCTTTCCACCACTGCAGGTTAGACTCAGATGAAACTTTCCGTCCTTCTGCATTAAAGCTTGTACGTGGAGAGGCTTTGGTTTTCAACTGCATGCTGAATTTACCTCACCTTAGTTACCGTGCACCGGAATCGGTTGCTTCATTCTTGAACGGAGCAAAAACGTTGAATCCAAAGCTTGTGACGTTGGTGGAAGAAGAAGTTGGTTCGGTAGTTGGTGGGTTTGTGGAAAGGTTCATGGACTCACTTCATCATTATTCAGCGGTTTTTGATTCATTGGAGGCTGGGTTTCCGATGCAGAACCGGGCCCGGGCTTTGGTGGAGAGGGTATTTTTTGGGCCTAGGATTGCAGGCTCGTTGGGCCGGATATATAGAACGGGTGGTGAAGAGGAGAGAAGGTCGTGGGGAGAGTGGTTAGGTGAGCTAGGGTTTAGGGGAGTTCCGGTGAGCTTTGCAAATCATTGTCAAGCAAAGCTGTTGTTAGGGCTTTTTAATGATGGGTATAGGGTTGAAGAGGTGGGTGTGGGTAGTAATAAGTTGGTGTTGGATTGGAAATCAAGACGTTTGCTTTCTGCTTCCCTTTGGACTTGTTCTTCTTCAGATTCTGATTTATAG
Protein sequence

>MS.gene031868.t1

MDMMDMDAINDLHFSGHSSLTNTPTSDDDYGCTWNHWSPIVNWDTFTGAPDDFHHLMDTIIEDRTTVLDQLSPSITTTTTTTTTDEEEEEMETTTTTTTTTEKTQEVGDDSKGLKLVHLLMAGAEALTGSTKNRDLARVILIRLKELVSQHANGSNMERLAAHFTEALHGLLEGAGGAHNNHHNNKHYLTTNGPHDNQNDTLAAFQLLQDMSPYVKFGHFTANQAIIEAVAHERRVHVIDYDIMEGVQWASLIQSLASNNNGPHLRITALSRTGTGRRSIATVQETGRRLTSFAASLGQPFSFHHCRLDSDETFRPSALKLVRGEALVFNCMLNLPHLSYRAPESVASFLNGAKTLNPKLVTLVEEEVGSVVGGFVERFMDSLHHYSAVFDSLEAGFPMQNRARALVERVFFGPRIAGSLGRIYRTGGEEERRSWGEWLGELGFRGVPVSFANHCQAKLLLGLFNDGYRVEEVGVGSNKLVLDWKSRRLLSASLWTCSSSDSDL