Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031894.t1 | XP_024637264.1 | 84.4 | 154 | 24 | 0 | 10 | 163 | 11 | 164 | 5.70E-74 | 287 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031894.t1 | G7JQ00 | 84.4 | 154 | 24 | 0 | 10 | 163 | 71 | 224 | 4.1e-74 | 287.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene031894.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031894.t1 | MTR_4g123470 | 84.416 | 154 | 24 | 0 | 10 | 163 | 71 | 224 | 2.24e-96 | 278 |
| MS.gene031894.t1 | MTR_7g113060 | 81.579 | 38 | 7 | 0 | 126 | 163 | 3 | 40 | 2.42e-16 | 72.8 |
| MS.gene031894.t1 | MTR_4g085790 | 27.815 | 151 | 101 | 3 | 3 | 149 | 61 | 207 | 1.57e-12 | 63.2 |
| MS.gene031894.t1 | MTR_7g025150 | 27.815 | 151 | 101 | 2 | 3 | 149 | 61 | 207 | 2.05e-12 | 63.2 |
| MS.gene031894.t1 | MTR_1g054495 | 29.655 | 145 | 92 | 5 | 4 | 143 | 62 | 201 | 4.18e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGGACTACCTCATACTTT+TGG | 0.338565 | 3.4:+71211283 | MS.gene031894:intron |
| CGATTGTTCTACGAGTTTAG+TGG | 0.409935 | 3.4:+71210995 | MS.gene031894:CDS |
| GTCATGTGTTTATCAACTCT+TGG | 0.410006 | 3.4:-71211521 | None:intergenic |
| ACCTTTGAGGTTCCTATCGT+TGG | 0.413727 | 3.4:+71211145 | MS.gene031894:CDS |
| TCCGAACATCACATTTGGTA+TGG | 0.417548 | 3.4:-71211398 | None:intergenic |
| ATCTACTTCCACCATGGTAC+TGG | 0.424919 | 3.4:-71211036 | None:intergenic |
| CCGAACATCACATTTGGTAT+GGG | 0.451435 | 3.4:-71211397 | None:intergenic |
| AAGAATTTGCCAAAAGTATG+AGG | 0.469320 | 3.4:-71211292 | None:intergenic |
| TGCCGCACTCATTGAATGAT+AGG | 0.473193 | 3.4:-71211107 | None:intergenic |
| CCAAATGTGATGTTCGGAAA+AGG | 0.475440 | 3.4:+71211403 | MS.gene031894:CDS |
| CCCATACCAAATGTGATGTT+CGG | 0.477035 | 3.4:+71211397 | MS.gene031894:CDS |
| TTGTGTTGCCAATGGTATTG+AGG | 0.514824 | 3.4:+71211438 | MS.gene031894:CDS |
| TCTATATATTTGAGTCTCAC+AGG | 0.539594 | 3.4:-71211370 | None:intergenic |
| CGAACATCACATTTGGTATG+GGG | 0.552019 | 3.4:-71211396 | None:intergenic |
| GTCTCCGGCCTCAATACCAT+TGG | 0.575295 | 3.4:-71211446 | None:intergenic |
| CTCATAACGTCGTTGACAGA+TGG | 0.587552 | 3.4:+71210959 | MS.gene031894:CDS |
| TTTCAAGTGCAAGTGTGTCA+TGG | 0.589305 | 3.4:+71211477 | MS.gene031894:CDS |
| TAACGTCGTTGACAGATGGT+TGG | 0.592218 | 3.4:+71210963 | MS.gene031894:CDS |
| TGACACTTGCTAGATGGTGA+TGG | 0.594754 | 3.4:+71210931 | None:intergenic |
| GTTGCCAATGGTATTGAGGC+CGG | 0.597504 | 3.4:+71211442 | MS.gene031894:CDS |
| ATGTGATGTTCGGAAAAGGT+TGG | 0.606400 | 3.4:+71211407 | MS.gene031894:CDS |
| GACACTTGCTAGATGGTGAT+GGG | 0.615365 | 3.4:+71210932 | None:intergenic |
| GCCGCACTCATTGAATGATA+GGG | 0.616026 | 3.4:-71211106 | None:intergenic |
| TCGATCGACGAAATCACCTA+AGG | 0.628108 | 3.4:+71211171 | MS.gene031894:CDS |
| ACCCTATCATTCAATGAGTG+CGG | 0.636716 | 3.4:+71211105 | MS.gene031894:CDS |
| GTCGATCGATATCCAACGAT+AGG | 0.637968 | 3.4:-71211157 | None:intergenic |
| CACGATTACGTAATGTTGTG+CGG | 0.649204 | 3.4:+71211328 | MS.gene031894:CDS |
| GTCGCTTTCCAGTACCATGG+TGG | 0.664216 | 3.4:+71211028 | MS.gene031894:CDS |
| GCACTCATTGAATGATAGGG+TGG | 0.666571 | 3.4:-71211103 | None:intergenic |
| TCCAACGATAGGAACCTCAA+AGG | 0.670850 | 3.4:-71211146 | None:intergenic |
| ATTGTCGCTTTCCAGTACCA+TGG | 0.681766 | 3.4:+71211025 | MS.gene031894:CDS |
| TTCGAAATCTACTTCCACCA+TGG | 0.688810 | 3.4:-71211042 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTTTTTTCTTTTTTTTA+GGG | + | chr3.4:71211265-71211284 | MS.gene031894:intron | 10.0% |
| !! | TTAGATATATGCTATAATAA+AGG | + | chr3.4:71211223-71211242 | MS.gene031894:intron | 15.0% |
| !!! | CAGTTTTTTTTCTTTTTTTT+AGG | + | chr3.4:71211264-71211283 | MS.gene031894:intron | 15.0% |
| !! | AAAAAACAATAAACAACCTT+AGG | - | chr3.4:71211190-71211209 | None:intergenic | 20.0% |
| TCTATATATTTGAGTCTCAC+AGG | - | chr3.4:71211373-71211392 | None:intergenic | 30.0% | |
| ! | AAGAATTTGCCAAAAGTATG+AGG | - | chr3.4:71211295-71211314 | None:intergenic | 30.0% |
| ! | ACTTTTGGCAAATTCTTTAG+AGG | + | chr3.4:71211298-71211317 | MS.gene031894:CDS | 30.0% |
| ! | CTTTTGGCAAATTCTTTAGA+GGG | + | chr3.4:71211299-71211318 | MS.gene031894:CDS | 30.0% |
| !! | ATCGTTTTATTGTACCTTTG+AGG | + | chr3.4:71211132-71211151 | MS.gene031894:CDS | 30.0% |
| ACCCTATCATTCAATGAGTG+CGG | + | chr3.4:71211105-71211124 | MS.gene031894:CDS | 40.0% | |
| ATGTGATGTTCGGAAAAGGT+TGG | + | chr3.4:71211407-71211426 | MS.gene031894:CDS | 40.0% | |
| CACGATTACGTAATGTTGTG+CGG | + | chr3.4:71211328-71211347 | MS.gene031894:CDS | 40.0% | |
| CCAAATGTGATGTTCGGAAA+AGG | + | chr3.4:71211403-71211422 | MS.gene031894:CDS | 40.0% | |
| CCCATACCAAATGTGATGTT+CGG | + | chr3.4:71211397-71211416 | MS.gene031894:CDS | 40.0% | |
| CCGAACATCACATTTGGTAT+GGG | - | chr3.4:71211400-71211419 | None:intergenic | 40.0% | |
| CGAACATCACATTTGGTATG+GGG | - | chr3.4:71211399-71211418 | None:intergenic | 40.0% | |
| CGATTGTTCTACGAGTTTAG+TGG | + | chr3.4:71210995-71211014 | MS.gene031894:CDS | 40.0% | |
| CTTGAAAAACAACACGTCTC+CGG | - | chr3.4:71211464-71211483 | None:intergenic | 40.0% | |
| TCCGAACATCACATTTGGTA+TGG | - | chr3.4:71211401-71211420 | None:intergenic | 40.0% | |
| TTCGAAATCTACTTCCACCA+TGG | - | chr3.4:71211045-71211064 | None:intergenic | 40.0% | |
| TTGTGTTGCCAATGGTATTG+AGG | + | chr3.4:71211438-71211457 | MS.gene031894:CDS | 40.0% | |
| ! | ACGAGTTTTTGTGTTGCCAA+TGG | + | chr3.4:71211430-71211449 | MS.gene031894:CDS | 40.0% |
| ! | CCTTTTCCGAACATCACATT+TGG | - | chr3.4:71211406-71211425 | None:intergenic | 40.0% |
| ! | TAGGGACTACCTCATACTTT+TGG | + | chr3.4:71211283-71211302 | MS.gene031894:intron | 40.0% |
| ! | TTTCAAGTGCAAGTGTGTCA+TGG | + | chr3.4:71211477-71211496 | MS.gene031894:CDS | 40.0% |
| ATCTACTTCCACCATGGTAC+TGG | - | chr3.4:71211039-71211058 | None:intergenic | 45.0% | |
| CTCATAACGTCGTTGACAGA+TGG | + | chr3.4:71210959-71210978 | MS.gene031894:CDS | 45.0% | |
| GCACTCATTGAATGATAGGG+TGG | - | chr3.4:71211106-71211125 | None:intergenic | 45.0% | |
| GCCGCACTCATTGAATGATA+GGG | - | chr3.4:71211109-71211128 | None:intergenic | 45.0% | |
| GTCGATCGATATCCAACGAT+AGG | - | chr3.4:71211160-71211179 | None:intergenic | 45.0% | |
| TAACGTCGTTGACAGATGGT+TGG | + | chr3.4:71210963-71210982 | MS.gene031894:CDS | 45.0% | |
| TCCAACGATAGGAACCTCAA+AGG | - | chr3.4:71211149-71211168 | None:intergenic | 45.0% | |
| TCGATCGACGAAATCACCTA+AGG | + | chr3.4:71211171-71211190 | MS.gene031894:CDS | 45.0% | |
| TGCCGCACTCATTGAATGAT+AGG | - | chr3.4:71211110-71211129 | None:intergenic | 45.0% | |
| !! | ACCTTTGAGGTTCCTATCGT+TGG | + | chr3.4:71211145-71211164 | MS.gene031894:CDS | 45.0% |
| !! | ATTGTCGCTTTCCAGTACCA+TGG | + | chr3.4:71211025-71211044 | MS.gene031894:CDS | 45.0% |
| ! | GTTGCCAATGGTATTGAGGC+CGG | + | chr3.4:71211442-71211461 | MS.gene031894:CDS | 50.0% |
| GTCTCCGGCCTCAATACCAT+TGG | - | chr3.4:71211449-71211468 | None:intergenic | 55.0% | |
| ! | GTCGCTTTCCAGTACCATGG+TGG | + | chr3.4:71211028-71211047 | MS.gene031894:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 71210944 | 71211531 | 71210944 | ID=MS.gene031894 |
| chr3.4 | mRNA | 71210944 | 71211531 | 71210944 | ID=MS.gene031894.t1;Parent=MS.gene031894 |
| chr3.4 | exon | 71210944 | 71211192 | 71210944 | ID=MS.gene031894.t1.exon1;Parent=MS.gene031894.t1 |
| chr3.4 | CDS | 71210944 | 71211192 | 71210944 | ID=cds.MS.gene031894.t1;Parent=MS.gene031894.t1 |
| chr3.4 | exon | 71211286 | 71211531 | 71211286 | ID=MS.gene031894.t1.exon2;Parent=MS.gene031894.t1 |
| chr3.4 | CDS | 71211286 | 71211531 | 71211286 | ID=cds.MS.gene031894.t1;Parent=MS.gene031894.t1 |
| Gene Sequence |
| Protein sequence |
