AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031929


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031929.t1 MTR_3g071590 99.375 160 0 1 1 160 1 159 5.14e-117 327
MS.gene031929.t1 MTR_5g083230 75.155 161 32 4 1 157 1 157 3.68e-81 237
MS.gene031929.t1 MTR_3g031660 65.359 153 41 1 3 143 2 154 7.22e-70 209
MS.gene031929.t1 MTR_2g068760 75.455 110 27 0 4 113 9 118 1.12e-57 177
MS.gene031929.t1 MTR_4g083680 60.811 148 48 3 2 145 5 146 6.00e-57 176
MS.gene031929.t1 MTR_2g093310 63.063 111 41 0 7 117 32 142 1.37e-50 160
MS.gene031929.t1 MTR_4g060950 65.179 112 38 1 7 117 23 134 1.13e-49 158
MS.gene031929.t1 MTR_1g095850 71.287 101 29 0 9 109 73 173 1.84e-48 156
MS.gene031929.t1 MTR_5g083960 67.619 105 34 0 4 108 35 139 6.14e-48 154
MS.gene031929.t1 MTR_3g094690 63.393 112 41 0 4 115 43 154 4.89e-47 153
MS.gene031929.t1 MTR_7g033800 59.829 117 43 1 5 117 8 124 4.03e-46 148
MS.gene031929.t1 MTR_6g005080 51.748 143 59 3 3 145 5 137 4.04e-46 147
MS.gene031929.t1 MTR_3g077240 66.346 104 35 0 6 109 8 111 8.16e-46 148
MS.gene031929.t1 MTR_6g005070 53.191 141 56 3 5 145 6 136 8.26e-46 147
MS.gene031929.t1 MTR_4g105170 66.019 103 35 0 7 109 10 112 1.20e-45 148
MS.gene031929.t1 MTR_3g073690 61.111 108 42 0 1 108 2 109 2.12e-45 147
MS.gene031929.t1 MTR_3g094690 62.500 112 41 1 4 115 43 153 1.89e-44 146
MS.gene031929.t1 MTR_3g071420 63.366 101 37 0 5 105 11 111 1.75e-42 139
MS.gene031929.t1 MTR_5g075020 66.038 106 36 0 4 109 39 144 1.97e-42 141
MS.gene031929.t1 MTR_3g452660 59.048 105 43 0 4 108 3 107 2.81e-42 142
MS.gene031929.t1 MTR_5g075020 66.038 106 36 0 4 109 43 148 3.01e-42 140
MS.gene031929.t1 MTR_5g017950 58.333 108 45 0 1 108 1 108 9.37e-42 142
MS.gene031929.t1 MTR_5g083010 62.376 101 38 0 5 105 8 108 2.25e-41 136
MS.gene031929.t1 MTR_7g096530 56.604 106 45 1 4 108 10 115 2.62e-37 127
MS.gene031929.t1 MTR_8g040900 59.615 104 42 0 5 108 4 107 8.26e-37 126
MS.gene031929.t1 MTR_1g070220 51.695 118 56 1 8 124 15 132 1.24e-36 125
MS.gene031929.t1 MTR_5g093010 42.143 140 74 2 9 141 6 145 2.59e-36 122
MS.gene031929.t1 MTR_7g074990 56.863 102 43 1 8 108 20 121 6.03e-36 124
MS.gene031929.t1 MTR_7g028905 52.941 102 48 0 7 108 4 105 1.79e-35 119
MS.gene031929.t1 MTR_7g096610 46.809 141 67 3 1 138 1 136 2.62e-35 122
MS.gene031929.t1 MTR_8g036105 56.075 107 46 1 7 112 17 123 6.63e-35 121
MS.gene031929.t1 MTR_7g075230 48.092 131 65 2 2 129 6 136 7.95e-35 120
MS.gene031929.t1 MTR_8g036085 54.369 103 46 1 7 108 22 124 3.47e-34 120
MS.gene031929.t1 MTR_6g027710 43.066 137 72 2 7 142 39 170 5.67e-34 119
MS.gene031929.t1 MTR_1g070205 45.517 145 69 6 8 147 12 151 1.29e-33 118
MS.gene031929.t1 MTR_5g080470 54.082 98 44 1 7 103 6 103 2.19e-33 117
MS.gene031929.t1 MTR_8g079660 47.525 101 53 0 8 108 8 108 1.62e-29 108
MS.gene031929.t1 MTR_8g079620 44.554 101 56 0 8 108 8 108 1.58e-28 105
MS.gene031929.t1 MTR_6g027700 46.535 101 53 1 8 107 7 107 2.28e-28 104
MS.gene031929.t1 MTR_8g079580 44.545 110 60 1 1 109 1 110 1.31e-27 103
MS.gene031929.t1 MTR_4g107450 42.742 124 64 2 1 117 3 126 4.72e-26 100
MS.gene031929.t1 MTR_6g011230 40.404 99 59 0 3 101 6 104 6.76e-26 97.8
MS.gene031929.t1 MTR_6g011250 38.686 137 77 3 9 144 10 140 2.50e-25 95.9
MS.gene031929.t1 MTR_2g100020 38.462 104 64 0 5 108 16 119 1.83e-22 90.5
MS.gene031929.t1 MTR_6g011200 43.750 80 44 1 9 87 10 89 5.96e-22 89.0
MS.gene031929.t1 MTR_8g017090 37.500 104 65 0 5 108 9 112 7.07e-22 89.7
MS.gene031929.t1 MTR_4g088035 38.202 89 55 0 7 95 5 93 3.90e-19 81.6
MS.gene031929.t1 MTR_4g099230 39.362 94 52 2 9 97 3 96 1.32e-18 77.8
MS.gene031929.t1 MTR_5g085390 42.500 80 45 1 9 87 10 89 2.35e-17 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031929.t1 AT2G30130 79.259 135 22 2 1 131 1 133 1.36e-72 216
MS.gene031929.t1 AT1G16530 67.544 114 36 1 3 115 9 122 3.53e-52 164
MS.gene031929.t1 AT1G07900 64.865 111 39 0 7 117 32 142 2.47e-50 160
MS.gene031929.t1 AT2G28500 65.138 109 38 0 7 115 54 162 9.21e-50 160
MS.gene031929.t1 AT1G31320 71.171 111 32 0 4 114 9 119 1.05e-49 157
MS.gene031929.t1 AT2G30340 64.151 106 38 0 4 109 48 153 2.71e-46 152
MS.gene031929.t1 AT2G30340 64.151 106 38 0 4 109 49 154 2.73e-46 152
MS.gene031929.t1 AT3G27650 62.385 109 41 0 1 109 32 140 5.13e-46 148
MS.gene031929.t1 AT5G63090 66.990 103 34 0 6 108 9 111 7.64e-46 148
MS.gene031929.t1 AT5G63090 66.990 103 34 0 6 108 9 111 7.64e-46 148
MS.gene031929.t1 AT5G63090 66.990 103 34 0 6 108 9 111 7.64e-46 148
MS.gene031929.t1 AT5G63090 66.990 103 34 0 6 108 9 111 7.64e-46 148
MS.gene031929.t1 AT3G26660 62.264 106 40 0 9 114 6 111 6.35e-45 144
MS.gene031929.t1 AT3G26620 62.264 106 40 0 9 114 6 111 7.00e-45 144
MS.gene031929.t1 AT2G40470 59.690 129 50 1 4 130 16 144 7.51e-45 146
MS.gene031929.t1 AT2G40470 59.690 129 50 1 4 130 41 169 1.27e-44 146
MS.gene031929.t1 AT3G11090 55.147 136 57 2 5 136 8 143 8.78e-44 142
MS.gene031929.t1 AT2G23660 56.731 104 45 0 6 109 3 106 2.23e-42 143
MS.gene031929.t1 AT2G23660 56.731 104 45 0 6 109 3 106 2.23e-42 143
MS.gene031929.t1 AT2G23660 56.731 104 45 0 6 109 3 106 2.23e-42 143
MS.gene031929.t1 AT5G66870 50.769 130 64 0 3 132 2 131 5.29e-42 142
MS.gene031929.t1 AT1G65620 61.905 105 40 0 4 108 5 109 7.34e-39 131
MS.gene031929.t1 AT1G65620 61.905 105 40 0 4 108 5 109 7.34e-39 131
MS.gene031929.t1 AT1G65620 61.905 105 40 0 4 108 5 109 7.34e-39 131
MS.gene031929.t1 AT1G65620 61.905 105 40 0 4 108 5 109 7.34e-39 131
MS.gene031929.t1 AT1G65620 61.905 105 40 0 4 108 5 109 7.34e-39 131
MS.gene031929.t1 AT4G00210 54.310 116 49 2 1 112 1 116 2.46e-37 127
MS.gene031929.t1 AT3G03760 55.769 104 45 1 7 109 50 153 7.85e-37 128
MS.gene031929.t1 AT2G42430 57.547 106 44 1 4 108 11 116 1.08e-36 127
MS.gene031929.t1 AT2G42440 47.244 127 65 2 7 132 6 131 1.39e-34 121
MS.gene031929.t1 AT2G45410 57.282 103 43 1 11 112 19 121 1.72e-34 119
MS.gene031929.t1 AT3G58190 54.639 97 43 1 8 103 11 107 3.83e-33 117
MS.gene031929.t1 AT2G31310 50.926 108 51 2 1 107 1 107 1.54e-32 114
MS.gene031929.t1 AT2G45420 51.923 104 49 1 11 113 40 143 1.99e-32 116
MS.gene031929.t1 AT4G00220 52.885 104 48 1 11 113 20 123 4.50e-32 114
MS.gene031929.t1 AT3G50510 43.902 123 67 1 6 126 10 132 2.81e-30 109
MS.gene031929.t1 AT3G50510 43.902 123 67 1 6 126 10 132 2.81e-30 109
MS.gene031929.t1 AT5G06080 51.376 109 47 2 7 114 6 109 7.00e-30 107
MS.gene031929.t1 AT5G35900 36.364 143 76 2 9 150 6 134 3.48e-26 99.0
MS.gene031929.t1 AT1G72980 38.525 122 74 1 3 123 8 129 4.73e-25 96.3
MS.gene031929.t1 AT3G47870 37.037 108 68 0 1 108 29 136 3.34e-24 96.3
MS.gene031929.t1 AT3G13850 35.537 121 74 1 9 125 37 157 2.35e-23 92.8
MS.gene031929.t1 AT1G06280 49.351 77 39 0 9 85 25 101 9.01e-21 84.7
MS.gene031929.t1 AT4G22700 37.500 112 67 3 9 119 6 115 2.33e-17 75.5
MS.gene031929.t1 AT2G19820 38.542 96 58 1 7 101 11 106 8.44e-17 72.4
MS.gene031929.t1 AT5G15060 40.000 80 44 2 3 79 12 90 5.64e-14 65.5
MS.gene031929.t1 AT1G36000 29.885 87 61 0 8 94 9 95 1.91e-12 60.8
MS.gene031929.t1 AT3G27940 36.364 66 42 0 7 72 4 69 2.91e-11 58.5
MS.gene031929.t1 AT3G27940 36.364 66 42 0 7 72 4 69 2.91e-11 58.5
MS.gene031929.t1 AT3G27940 36.364 66 42 0 7 72 4 69 2.91e-11 58.5

Find 21 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTAGCACAACGGCGTCTA+AGG 0.285930 3.4:-70837326 None:intergenic
GCATCAGCTCTTTGTTGAAT+AGG 0.314081 3.4:-70837976 None:intergenic
GGATCATGTGAAGGGAAATA+AGG 0.350330 3.4:-70837363 None:intergenic
GTTGATCATCATGTTTCATC+TGG 0.383715 3.4:-70838140 None:intergenic
AGAGTGAAAGACCCTGTTTA+TGG 0.387679 3.4:+70838030 MS.gene031929:CDS
GCTCCAACACAACCATAAAC+AGG 0.400328 3.4:-70838042 None:intergenic
GCATTTGAAGCTCAGAAACT+TGG 0.418459 3.4:-70838083 None:intergenic
TGAATTACATTGCTTGAAGA+AGG 0.450118 3.4:-70838234 None:intergenic
TACTGAGGAAGCTGATCATA+AGG 0.470175 3.4:-70838201 None:intergenic
GATCATAAGGTTTATGATCA+AGG 0.470200 3.4:-70838188 None:intergenic
GACCCTGTTTATGGTTGTGT+TGG 0.486001 3.4:+70838039 MS.gene031929:CDS
CCTTAGACGCCGTTGTGCTA+AGG 0.517412 3.4:+70837326 MS.gene031929:CDS
ATCATAAGGTTTATGATCAA+GGG 0.563088 3.4:-70838187 None:intergenic
CAACGTTAGCAAAATGCTCC+AGG 0.569502 3.4:+70837425 MS.gene031929:CDS
GAAGGAAAATTGAAATACTG+AGG 0.570319 3.4:-70838216 None:intergenic
AAAATTTGCAATAGTTCACA+AGG 0.586403 3.4:+70837389 MS.gene031929:CDS
AGGAGTTTGCATGAAGCACA+AGG 0.592427 3.4:-70837306 None:intergenic
GAGTTTGCATGAAGCACAAG+GGG 0.629954 3.4:-70837304 None:intergenic
CTCCAACACAACCATAAACA+GGG 0.645133 3.4:-70838041 None:intergenic
GGAGTTTGCATGAAGCACAA+GGG 0.671143 3.4:-70837305 None:intergenic
AGATACAATCCTTAGCACAA+CGG 0.718268 3.4:-70837335 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAACTTTTTTTTTAAAGAA+AGG + chr3.4:70837914-70837933 MS.gene031929:intron 10.0%
!!! TCGTTTTTAAAATAGAATAT+TGG - chr3.4:70837803-70837822 None:intergenic 15.0%
!!! TTTTTTTTAAAGAAAGGAAA+TGG + chr3.4:70837920-70837939 MS.gene031929:intron 15.0%
!! AAATGATATGACATTAAACA+TGG - chr3.4:70837735-70837754 None:intergenic 20.0%
!! ATGTCATATCATTTAACAAT+TGG + chr3.4:70837741-70837760 MS.gene031929:intron 20.0%
!!! AAAACTTCGATTTTAAAATG+TGG - chr3.4:70837599-70837618 None:intergenic 20.0%
!!! AAAAGTGCAATTTTCTTATT+AGG + chr3.4:70837859-70837878 MS.gene031929:intron 20.0%
!!! AAGAAAAAAGTACAATTAAG+CGG - chr3.4:70837548-70837567 None:intergenic 20.0%
!!! AAGTTTTTCTTTTTCTTTCA+AGG - chr3.4:70837476-70837495 None:intergenic 20.0%
!!! TGAACTATTGCAAATTTTTT+AGG - chr3.4:70837387-70837406 None:intergenic 20.0%
! AAAATTTGCAATAGTTCACA+AGG + chr3.4:70837389-70837408 MS.gene031929:CDS 25.0%
! ATCATAAGGTTTATGATCAA+GGG - chr3.4:70838190-70838209 None:intergenic 25.0%
! CAGATATTTGTTTCAAAAAG+TGG - chr3.4:70837772-70837791 None:intergenic 25.0%
!! ATTTCTTGATTTTGCATTAG+AGG - chr3.4:70838165-70838184 None:intergenic 25.0%
!!! AATTTTTTAGGATCATGTGA+AGG - chr3.4:70837375-70837394 None:intergenic 25.0%
!!! ATTTTTTAGGATCATGTGAA+GGG - chr3.4:70837374-70837393 None:intergenic 25.0%
!!! TATTAGGTTTTGAGTTTTGA+CGG + chr3.4:70837875-70837894 MS.gene031929:intron 25.0%
ATTTGTTTCAAAAAGTGGTG+TGG - chr3.4:70837767-70837786 None:intergenic 30.0%
GAAGGAAAATTGAAATACTG+AGG - chr3.4:70838219-70838238 None:intergenic 30.0%
GACTTATTGTACAATGCTAT+AGG + chr3.4:70837948-70837967 MS.gene031929:intron 30.0%
GATCATAAGGTTTATGATCA+AGG - chr3.4:70838191-70838210 None:intergenic 30.0%
TGAATTACATTGCTTGAAGA+AGG - chr3.4:70838237-70838256 None:intergenic 30.0%
!!! GTATAAGAGAGCGTTATTTT+AGG + chr3.4:70837504-70837523 MS.gene031929:intron 30.0%
!!! TTTTGAGTTTTGACGGTTTT+GGG + chr3.4:70837882-70837901 MS.gene031929:intron 30.0%
AGATACAATCCTTAGCACAA+CGG - chr3.4:70837338-70837357 None:intergenic 35.0%
CACCATTAGAGCAAAAATCT+GGG - chr3.4:70837661-70837680 None:intergenic 35.0%
GTTGATCATCATGTTTCATC+TGG - chr3.4:70838143-70838162 None:intergenic 35.0%
TTAGAGCAAAAATCTGGGTT+AGG - chr3.4:70837656-70837675 None:intergenic 35.0%
! AACCCAGATTTTTGCTCTAA+TGG + chr3.4:70837656-70837675 MS.gene031929:intron 35.0%
! GCAATAGTTCACAAGGTTTT+TGG + chr3.4:70837396-70837415 MS.gene031929:CDS 35.0%
! TATCAAACTCGTTGCAGTTT+TGG + chr3.4:70837629-70837648 MS.gene031929:intron 35.0%
!!! AGAAACTTGGTTTTGAAGGT+AGG - chr3.4:70838073-70838092 None:intergenic 35.0%
!!! GATGACTATTTTTGTGAACG+CGG + chr3.4:70837821-70837840 MS.gene031929:intron 35.0%
!!! GTTTTGAGTTTTGACGGTTT+TGG + chr3.4:70837881-70837900 MS.gene031929:intron 35.0%
!!! TTGGTTTTGAAGGTAGGATA+TGG - chr3.4:70838067-70838086 None:intergenic 35.0%
AGAGTGAAAGACCCTGTTTA+TGG + chr3.4:70838030-70838049 MS.gene031929:CDS 40.0%
CCACCATTAGAGCAAAAATC+TGG - chr3.4:70837662-70837681 None:intergenic 40.0%
CTCCAACACAACCATAAACA+GGG - chr3.4:70838044-70838063 None:intergenic 40.0%
GCATCAGCTCTTTGTTGAAT+AGG - chr3.4:70837979-70837998 None:intergenic 40.0%
GGATCATGTGAAGGGAAATA+AGG - chr3.4:70837366-70837385 None:intergenic 40.0%
TACTGAGGAAGCTGATCATA+AGG - chr3.4:70838204-70838223 None:intergenic 40.0%
! CCAGATTTTTGCTCTAATGG+TGG + chr3.4:70837659-70837678 MS.gene031929:intron 40.0%
! GCATTTGAAGCTCAGAAACT+TGG - chr3.4:70838086-70838105 None:intergenic 40.0%
!!! GCTCAGAAACTTGGTTTTGA+AGG - chr3.4:70838077-70838096 None:intergenic 40.0%
!!! TTGACGGTTTTGGGTGAAAA+TGG + chr3.4:70837891-70837910 MS.gene031929:intron 40.0%
AGGAGTTTGCATGAAGCACA+AGG - chr3.4:70837309-70837328 None:intergenic 45.0%
CAACGTTAGCAAAATGCTCC+AGG + chr3.4:70837425-70837444 MS.gene031929:CDS 45.0%
GACCCTGTTTATGGTTGTGT+TGG + chr3.4:70838039-70838058 MS.gene031929:CDS 45.0%
GAGTTTGCATGAAGCACAAG+GGG - chr3.4:70837307-70837326 None:intergenic 45.0%
GCTCCAACACAACCATAAAC+AGG - chr3.4:70838045-70838064 None:intergenic 45.0%
GGAGTTTGCATGAAGCACAA+GGG - chr3.4:70837308-70837327 None:intergenic 45.0%
! GAGCATTTTGCTAACGTTGC+TGG - chr3.4:70837424-70837443 None:intergenic 45.0%
! TCAAGGAAGCATAGAAAGCG+TGG - chr3.4:70837459-70837478 None:intergenic 45.0%
! GAAAGCGTGGATGAAGTACC+TGG - chr3.4:70837446-70837465 None:intergenic 50.0%
CCTTAGACGCCGTTGTGCTA+AGG + chr3.4:70837326-70837345 MS.gene031929:CDS 55.0%
CCTTAGCACAACGGCGTCTA+AGG - chr3.4:70837329-70837348 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 70837285 70838290 70837285 ID=MS.gene031929
chr3.4 mRNA 70837285 70838290 70837285 ID=MS.gene031929.t1;Parent=MS.gene031929
chr3.4 exon 70837285 70837446 70837285 ID=MS.gene031929.t1.exon1;Parent=MS.gene031929.t1
chr3.4 CDS 70837285 70837446 70837285 ID=cds.MS.gene031929.t1;Parent=MS.gene031929.t1
chr3.4 exon 70837970 70838290 70837970 ID=MS.gene031929.t1.exon2;Parent=MS.gene031929.t1
chr3.4 CDS 70837970 70838290 70837970 ID=cds.MS.gene031929.t1;Parent=MS.gene031929.t1
Gene Sequence

>MS.gene031929

ATGGGTGGTAATACTACTTCCCCTTGTGCTTCATGCAAACTCCTTAGACGCCGTTGTGCTAAGGATTGTATCTTTGCTCCTTATTTCCCTTCACATGATCCTAAAAAATTTGCAATAGTTCACAAGGTTTTTGGTGCCAGCAACGTTAGCAAAATGCTCCAGGTACTTCATCCACGCTTTCTATGCTTCCTTGAAAGAAAAAGAAAAACTTCATAGTGTGTATAAGAGAGCGTTATTTTAGGTTTAGTTTTATTATCTTTCCGCTTAATTGTACTTTTTTCTTCTTATTTTATCATGTTCACGAAAATAGTCCACATTTTAAAATCGAAGTTTTTGTCTTCAAGTATCAAACTCGTTGCAGTTTTGGTCCTAACCCAGATTTTTGCTCTAATGGTGGTGTGTCTTGTTTATAATTTCAACTATTTGTAATTTATTTTTGTTACGACACCATGTTTAATGTCATATCATTTAACAATTGGCCACACCACTTTTTGAAACAAATATCTGCGTTTAAACCAATATTCTATTTTAAAAACGATGACTATTTTTGTGAACGCGGTAAAATAGAAAGATCAAAAGTGCAATTTTCTTATTAGGTTTTGAGTTTTGACGGTTTTGGGTGAAAATGGTTAACTTTTTTTTTAAAGAAAGGAAATGGTTATTGACTTATTGTACAATGCTATAGGAACTTCCTATTCAACAAAGAGCTGATGCAGTAAGCAGTTTAGTATATGAAGCAAATGCAAGAGTGAAAGACCCTGTTTATGGTTGTGTTGGAGCCATATCCTACCTTCAAAACCAAGTTTCTGAGCTTCAAATGCAGCTTGCAGTAGCACAAGCAGAAATACTCTGTATCCAGATGAAACATGATGATCAACCTCTAATGCAAAATCAAGAAATAGCCCTTGATCATAAACCTTATGATCAGCTTCCTCAGTATTTCAATTTTCCTTCTTCAAGCAATGTAATTCATCATGATTCTTTCTCAAGAGAGAGAATTTTTTGA

Protein sequence

>MS.gene031929.t1

MGGNTTSPCASCKLLRRRCAKDCIFAPYFPSHDPKKFAIVHKVFGASNVSKMLQELPIQQRADAVSSLVYEANARVKDPVYGCVGAISYLQNQVSELQMQLAVAQAEILCIQMKHDDQPLMQNQEIALDHKPYDQLPQYFNFPSSSNVIHHDSFSRERIF