AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031943


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031943.t1 MTR_3g071420 91.275 149 8 1 1 144 1 149 1.62e-80 235
MS.gene031943.t1 MTR_5g083010 76.596 141 33 0 4 144 1 141 1.05e-74 220
MS.gene031943.t1 MTR_7g033800 64.151 106 38 0 11 116 8 113 1.04e-46 149
MS.gene031943.t1 MTR_6g005070 65.094 106 37 0 11 116 6 111 2.54e-46 147
MS.gene031943.t1 MTR_5g083230 64.078 103 37 0 12 114 4 106 1.54e-44 144
MS.gene031943.t1 MTR_3g031660 52.000 125 59 1 12 136 5 128 7.36e-44 142
MS.gene031943.t1 MTR_4g105170 60.952 105 41 0 12 116 9 113 8.36e-44 142
MS.gene031943.t1 MTR_3g071590 61.905 105 40 0 10 114 4 108 8.37e-44 141
MS.gene031943.t1 MTR_6g005080 62.264 106 40 0 11 116 7 112 9.23e-44 141
MS.gene031943.t1 MTR_4g083680 52.846 123 58 0 11 133 12 134 1.35e-43 141
MS.gene031943.t1 MTR_3g077240 61.905 105 40 0 12 116 8 112 1.47e-43 142
MS.gene031943.t1 MTR_5g083960 49.630 135 62 1 11 145 36 164 1.23e-42 140
MS.gene031943.t1 MTR_3g073690 61.905 105 40 0 11 115 6 110 2.46e-42 139
MS.gene031943.t1 MTR_2g068760 58.491 106 44 0 6 111 5 110 4.58e-41 134
MS.gene031943.t1 MTR_5g017950 56.303 119 52 0 11 129 5 123 6.77e-41 139
MS.gene031943.t1 MTR_3g452660 60.000 105 42 0 11 115 4 108 1.29e-40 137
MS.gene031943.t1 MTR_8g040900 58.120 117 47 1 11 125 4 120 1.80e-39 132
MS.gene031943.t1 MTR_3g094690 55.046 109 49 0 15 123 48 156 1.15e-37 128
MS.gene031943.t1 MTR_5g075020 55.263 114 51 0 15 128 44 157 9.21e-37 126
MS.gene031943.t1 MTR_5g075020 54.310 116 53 0 13 128 46 161 1.55e-36 125
MS.gene031943.t1 MTR_2g093310 54.902 102 46 0 13 114 32 133 4.63e-36 123
MS.gene031943.t1 MTR_3g094690 54.128 109 49 1 15 123 48 155 3.90e-35 122
MS.gene031943.t1 MTR_1g095850 46.923 130 66 2 15 141 73 202 9.73e-35 120
MS.gene031943.t1 MTR_1g070220 50.435 115 55 2 1 113 1 115 1.65e-34 119
MS.gene031943.t1 MTR_7g096530 47.863 117 60 1 1 116 1 117 1.80e-34 119
MS.gene031943.t1 MTR_4g060950 56.701 97 41 1 13 108 23 119 2.29e-34 119
MS.gene031943.t1 MTR_8g036085 52.941 102 47 1 15 115 24 125 3.70e-34 119
MS.gene031943.t1 MTR_8g036105 49.558 113 56 1 4 115 8 120 5.05e-34 118
MS.gene031943.t1 MTR_7g074990 51.485 101 48 1 15 114 21 121 8.07e-34 118
MS.gene031943.t1 MTR_6g027710 41.985 131 73 2 15 144 41 169 1.02e-32 115
MS.gene031943.t1 MTR_7g096610 49.541 109 53 2 11 117 5 113 3.30e-32 114
MS.gene031943.t1 MTR_7g028905 44.660 103 57 0 13 115 4 106 7.37e-32 110
MS.gene031943.t1 MTR_1g070205 48.544 103 52 1 15 116 13 115 7.73e-31 110
MS.gene031943.t1 MTR_5g080470 45.283 106 57 1 12 116 5 110 8.09e-31 109
MS.gene031943.t1 MTR_5g093010 43.564 101 57 0 15 115 6 106 8.85e-30 105
MS.gene031943.t1 MTR_7g075230 50.000 104 49 2 15 115 13 116 7.11e-29 105
MS.gene031943.t1 MTR_8g079660 48.039 102 53 0 15 116 9 110 4.43e-28 104
MS.gene031943.t1 MTR_6g027700 40.517 116 68 1 15 129 8 123 6.23e-27 100
MS.gene031943.t1 MTR_8g079620 44.118 102 57 0 15 116 9 110 1.85e-24 94.7
MS.gene031943.t1 MTR_4g107450 39.815 108 65 0 11 118 7 114 1.09e-23 93.6
MS.gene031943.t1 MTR_6g011230 35.484 124 79 1 11 133 8 131 1.78e-22 88.6
MS.gene031943.t1 MTR_8g079580 37.600 125 75 2 15 138 9 131 4.35e-22 88.2
MS.gene031943.t1 MTR_6g011200 37.273 110 68 1 10 118 5 114 1.76e-21 87.0
MS.gene031943.t1 MTR_6g011250 36.364 110 69 1 10 118 5 114 1.37e-20 83.2
MS.gene031943.t1 MTR_2g100020 32.727 110 74 0 11 120 16 125 2.03e-20 84.7
MS.gene031943.t1 MTR_8g017090 33.333 108 72 0 11 118 9 116 7.79e-20 83.6
MS.gene031943.t1 MTR_4g099230 32.231 121 73 3 15 130 3 119 5.19e-19 78.6
MS.gene031943.t1 MTR_5g085390 34.400 125 73 3 10 129 5 125 7.54e-18 77.0
MS.gene031943.t1 MTR_4g088035 31.111 90 62 0 11 100 3 92 7.59e-14 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031943.t1 AT3G11090 66.667 138 46 0 4 141 1 138 9.85e-63 190
MS.gene031943.t1 AT3G27650 64.151 106 38 0 11 116 36 141 2.37e-45 145
MS.gene031943.t1 AT5G66870 62.857 105 39 0 11 115 4 108 1.02e-43 146
MS.gene031943.t1 AT5G63090 58.407 113 47 0 12 124 9 121 2.89e-43 141
MS.gene031943.t1 AT5G63090 58.407 113 47 0 12 124 9 121 2.89e-43 141
MS.gene031943.t1 AT5G63090 58.407 113 47 0 12 124 9 121 2.89e-43 141
MS.gene031943.t1 AT5G63090 58.407 113 47 0 12 124 9 121 2.89e-43 141
MS.gene031943.t1 AT2G23660 60.577 104 41 0 11 114 2 105 2.04e-42 142
MS.gene031943.t1 AT2G23660 60.577 104 41 0 11 114 2 105 2.04e-42 142
MS.gene031943.t1 AT2G23660 60.577 104 41 0 11 114 2 105 2.04e-42 142
MS.gene031943.t1 AT2G30130 59.615 104 42 0 11 114 5 108 6.75e-42 138
MS.gene031943.t1 AT1G16530 49.630 135 61 2 10 137 10 144 3.81e-40 132
MS.gene031943.t1 AT2G30340 55.263 114 50 1 15 128 54 166 4.85e-39 133
MS.gene031943.t1 AT2G30340 55.263 114 50 1 15 128 53 165 5.05e-39 132
MS.gene031943.t1 AT1G65620 56.140 114 50 0 11 124 6 119 9.03e-39 130
MS.gene031943.t1 AT1G65620 56.140 114 50 0 11 124 6 119 9.03e-39 130
MS.gene031943.t1 AT1G65620 56.140 114 50 0 11 124 6 119 9.03e-39 130
MS.gene031943.t1 AT1G65620 56.140 114 50 0 11 124 6 119 9.03e-39 130
MS.gene031943.t1 AT1G65620 56.140 114 50 0 11 124 6 119 9.03e-39 130
MS.gene031943.t1 AT1G31320 60.000 90 36 0 11 100 10 99 1.54e-37 126
MS.gene031943.t1 AT2G40470 53.846 117 54 0 15 131 21 137 1.60e-37 127
MS.gene031943.t1 AT2G40470 53.846 117 54 0 15 131 46 162 2.80e-37 127
MS.gene031943.t1 AT1G07900 58.586 99 41 0 13 111 32 130 3.66e-37 125
MS.gene031943.t1 AT2G28500 58.333 96 40 0 13 108 54 149 6.64e-36 124
MS.gene031943.t1 AT4G00210 52.336 107 50 1 15 120 12 118 4.38e-35 121
MS.gene031943.t1 AT3G03760 48.649 111 56 1 13 122 50 160 7.91e-35 122
MS.gene031943.t1 AT4G00220 43.056 144 65 2 17 143 20 163 1.25e-33 118
MS.gene031943.t1 AT3G26660 48.515 101 52 0 15 115 6 106 1.64e-33 114
MS.gene031943.t1 AT2G42430 47.500 120 62 1 5 123 6 125 1.66e-33 118
MS.gene031943.t1 AT3G26620 48.515 101 52 0 15 115 6 106 2.04e-33 114
MS.gene031943.t1 AT2G45420 50.000 100 49 1 17 115 40 139 1.38e-32 116
MS.gene031943.t1 AT3G58190 43.443 122 65 2 15 135 12 130 2.29e-31 112
MS.gene031943.t1 AT2G42440 47.115 104 54 1 11 113 4 107 9.55e-31 110
MS.gene031943.t1 AT5G06080 45.631 103 55 1 13 114 6 108 1.97e-30 108
MS.gene031943.t1 AT2G45410 51.000 100 48 1 17 115 19 118 2.46e-30 108
MS.gene031943.t1 AT2G31310 43.396 106 59 1 12 116 5 110 1.16e-29 106
MS.gene031943.t1 AT3G50510 47.115 104 55 0 12 115 10 113 2.78e-28 103
MS.gene031943.t1 AT3G50510 47.115 104 55 0 12 115 10 113 2.78e-28 103
MS.gene031943.t1 AT5G35900 36.000 125 80 0 11 135 2 126 3.48e-27 100
MS.gene031943.t1 AT3G13850 37.963 108 67 0 14 121 36 143 1.59e-23 92.8
MS.gene031943.t1 AT1G72980 34.677 124 81 0 11 134 10 133 2.62e-22 88.6
MS.gene031943.t1 AT3G47870 34.862 109 71 0 11 119 33 141 2.44e-19 82.4
MS.gene031943.t1 AT4G22700 39.252 107 61 3 11 115 2 106 6.90e-19 79.0
MS.gene031943.t1 AT1G06280 44.872 78 43 0 14 91 24 101 2.56e-18 77.8
MS.gene031943.t1 AT2G19820 35.632 87 55 1 15 100 13 99 9.46e-13 61.2
MS.gene031943.t1 AT5G15060 39.474 76 42 2 13 85 16 90 1.01e-12 61.6

Find 22 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAAAGTTCACAAAGTATT+TGG 0.136847 3.4:-70666231 MS.gene031943:CDS
TAATTTCATGGTCAGGATTC+AGG 0.234257 3.4:+70665575 None:intergenic
AGGCTTAAAGACCCAGTTTA+TGG 0.242004 3.4:-70666120 MS.gene031943:CDS
GAACCAATGCATCCATAAAC+TGG 0.317210 3.4:+70666108 None:intergenic
GATGGAACTTCAACATGATT+TGG 0.337444 3.4:-70666061 MS.gene031943:CDS
AAGTGAAATGAAAGGCATAA+AGG 0.382977 3.4:-70666367 None:intergenic
ATTTCTTGCACTCGTTAGAA+TGG 0.427400 3.4:+70666256 None:intergenic
GACCCAGTTTATGGATGCAT+TGG 0.436697 3.4:-70666111 MS.gene031943:CDS
AGTGAAATGAAAGGCATAAA+GGG 0.450119 3.4:-70666366 None:intergenic
TAAGGTGCAAATAAGCAATC+TGG 0.484939 3.4:+70666282 None:intergenic
CTTCCTTTGCAACATAGCTA+TGG 0.497960 3.4:+70666086 None:intergenic
ACATGATTTGGCCATTGCTA+AGG 0.501000 3.4:-70666049 MS.gene031943:CDS
CACTCGTTAGAATGGAAGTA+AGG 0.514845 3.4:+70666264 None:intergenic
TATGTTGCAAAGGAAGATGA+TGG 0.575914 3.4:-70666079 MS.gene031943:CDS
GTTCCATAGCTATGTTGCAA+AGG 0.579187 3.4:-70666089 MS.gene031943:CDS
AGCAAGACGTTCCTTAGCAA+TGG 0.581078 3.4:+70666038 None:intergenic
AATTACAGAAGGAGTTGTTG+TGG 0.604155 3.4:+70665978 None:intergenic
TTGCTTATGAAGCAGAAGCA+AGG 0.610245 3.4:-70666140 MS.gene031943:CDS
AACCAATGCATCCATAAACT+GGG 0.613188 3.4:+70666109 None:intergenic
GTGCACACAAGTGAAATGAA+AGG 0.632849 3.4:-70666375 None:intergenic
GCACAAGATGAGCTTGAACG+TGG 0.651989 3.4:+70666336 None:intergenic
TACAGAAGGAGTTGTTGTGG+TGG 0.695339 3.4:+70665981 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTAATTCACAAAAAAG+GGG + chr3.4:70666158-70666177 None:intergenic 15.0%
!!! ACAATTTTAATTCACAAAAA+AGG + chr3.4:70666160-70666179 None:intergenic 15.0%
!!! CAATTTTAATTCACAAAAAA+GGG + chr3.4:70666159-70666178 None:intergenic 15.0%
!! TTATCTCTTATAACAATTAC+CGG + chr3.4:70666271-70666290 None:intergenic 20.0%
!!! ATTTTAATTCACAAAAAAGG+GGG + chr3.4:70666157-70666176 None:intergenic 20.0%
! CCATATGATCAAAATGTATA+AGG + chr3.4:70666132-70666151 None:intergenic 25.0%
! GTGTTAATACATAATTTCCA+TGG - chr3.4:70666243-70666262 MS.gene031943:CDS 25.0%
!!! CCTTATACATTTTGATCATA+TGG - chr3.4:70666129-70666148 MS.gene031943:CDS 25.0%
!!! GCTGGAAAATATTGTTTTTT+TGG - chr3.4:70666296-70666315 MS.gene031943:CDS 25.0%
!!! TTTTAATTCACAAAAAAGGG+GGG + chr3.4:70666156-70666175 None:intergenic 25.0%
ATACATAATTTCCATGGTTC+CGG - chr3.4:70666249-70666268 MS.gene031943:CDS 30.0%
TGCAAACTATCAATTACAGA+AGG + chr3.4:70665982-70666001 None:intergenic 30.0%
TTATAAGAGATAAGAACTGC+TGG - chr3.4:70666278-70666297 MS.gene031943:CDS 30.0%
! CTCAATGATATCTTTTGTCA+TGG - chr3.4:70666051-70666070 MS.gene031943:CDS 30.0%
! GCTAAAGTTCACAAAGTATT+TGG - chr3.4:70665715-70665734 MS.gene031943:intron 30.0%
AACCAATGCATCCATAAACT+GGG + chr3.4:70665840-70665859 None:intergenic 35.0%
AATTACAGAAGGAGTTGTTG+TGG + chr3.4:70665971-70665990 None:intergenic 35.0%
ATCATTGAGATCGTTAGAAG+TGG + chr3.4:70666040-70666059 None:intergenic 35.0%
ATTTCTTGCACTCGTTAGAA+TGG + chr3.4:70665693-70665712 None:intergenic 35.0%
GATGGAACTTCAACATGATT+TGG - chr3.4:70665885-70665904 MS.gene031943:intron 35.0%
TAAGGTGCAAATAAGCAATC+TGG + chr3.4:70665667-70665686 None:intergenic 35.0%
TATAACAATTACCGGAACCA+TGG + chr3.4:70666263-70666282 None:intergenic 35.0%
TATGTTGCAAAGGAAGATGA+TGG - chr3.4:70665867-70665886 MS.gene031943:intron 35.0%
TTTGGGTCGAAATGAAACAA+TGG - chr3.4:70666089-70666108 MS.gene031943:CDS 35.0%
! GCGATATGATTTTTCACTGT+TGG - chr3.4:70666343-70666362 MS.gene031943:CDS 35.0%
!!! GTGTCTTCTCTTTGTTTTTC+TGG + chr3.4:70665775-70665794 None:intergenic 35.0%
AGGCTTAAAGACCCAGTTTA+TGG - chr3.4:70665826-70665845 MS.gene031943:intron 40.0%
CACTCGTTAGAATGGAAGTA+AGG + chr3.4:70665685-70665704 None:intergenic 40.0%
CTTCCTTTGCAACATAGCTA+TGG + chr3.4:70665863-70665882 None:intergenic 40.0%
GAACCAATGCATCCATAAAC+TGG + chr3.4:70665841-70665860 None:intergenic 40.0%
GAAGTGGAAAACTCAGGAAA+TGG + chr3.4:70666024-70666043 None:intergenic 40.0%
GTTCCATAGCTATGTTGCAA+AGG - chr3.4:70665857-70665876 MS.gene031943:intron 40.0%
TCGTTAGAAGTGGAAAACTC+AGG + chr3.4:70666030-70666049 None:intergenic 40.0%
TTGCTTATGAAGCAGAAGCA+AGG - chr3.4:70665806-70665825 MS.gene031943:intron 40.0%
! ACATGATTTGGCCATTGCTA+AGG - chr3.4:70665897-70665916 MS.gene031943:intron 40.0%
! GGCTCTTCTTCGCAATTTTT+GGG - chr3.4:70666072-70666091 MS.gene031943:CDS 40.0%
! TGGCTCTTCTTCGCAATTTT+TGG - chr3.4:70666071-70666090 MS.gene031943:CDS 40.0%
AGCAAGACGTTCCTTAGCAA+TGG + chr3.4:70665911-70665930 None:intergenic 45.0%
GACCCAGTTTATGGATGCAT+TGG - chr3.4:70665835-70665854 MS.gene031943:intron 45.0%
GTGGAAAACTCAGGAAATGG+AGG + chr3.4:70666021-70666040 None:intergenic 45.0%
TACAGAAGGAGTTGTTGTGG+TGG + chr3.4:70665968-70665987 None:intergenic 45.0%
GCACAAGATGAGCTTGAACG+TGG + chr3.4:70665613-70665632 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 70665586 70666382 70665586 ID=MS.gene031943
chr3.4 mRNA 70665586 70666382 70665586 ID=MS.gene031943.t1;Parent=MS.gene031943
chr3.4 exon 70665984 70666382 70665984 ID=MS.gene031943.t1.exon1;Parent=MS.gene031943.t1
chr3.4 CDS 70665984 70666382 70665984 ID=cds.MS.gene031943.t1;Parent=MS.gene031943.t1
chr3.4 exon 70665586 70665624 70665586 ID=MS.gene031943.t1.exon2;Parent=MS.gene031943.t1
chr3.4 CDS 70665586 70665624 70665586 ID=cds.MS.gene031943.t1;Parent=MS.gene031943.t1
Gene Sequence

>MS.gene031943

ATGAAAGGCATAAAGGGTTATGAGCCACGTTCAAGCTCATCTTGTGCAGCTTGCAAATTATTGAAAAGAAGATGCACACCAGATTGCTTATTTGCACCTTACTTCCATTCTAACGAGTGCAAGAAATTTGCTAAAGTTCACAAAGTATTTGGAGCAAGCAATGTTAGCAAGATACTCATTGAAGTACCAGAAAAACAAAGAGAAGACACAGTGAATTCTCTTGCTTATGAAGCAGAAGCAAGGCTTAAAGACCCAGTTTATGGATGCATTGGTTCCATAGCTATGTTGCAAAGGAAGATGATGGAACTTCAACATGATTTGGCCATTGCTAAGGAACGTCTTGCTCGCTGTCGTGCTGTTGCTGCAGCTGCTACAATCACCACCACAACAACTCCTTCTGTAATTGATAGTTTGCATTCTGATGTTACTTTGCCTCCATTTCCTGAGTTTTCCACTTCTAACGATCTCAATGATATCTTTTGTCATGGCTCTTCTTCGCAATTTTTGGGTCGAAATGAAACAATGGATGATTTCAATCAAATTCCTTATACATTTTGATCATATGGTCCCCCCTTTTTTGTGAATTAAAATTGTCATGTATGTGCTAATAAATAAGAACTTCATGATTAGCAATGAAAAAAAAACATGTATTATTGTGTGTTAATACATAATTTCCATGGTTCCGGTAATTGTTATAAGAGATAAGAACTGCTGGAAAATATTGTTTTTTTGGTCATTATGTTTCAATTTTGTAACAGCGATATGATTTTTCACTGTTGGTGTTTCCTGAATCCTGA

Protein sequence

>MS.gene031943.t1

MKGIKGYEPRSSSSCAACKLLKRRCTPDCLFAPYFHSNECKKFAKVHKVFGASNVSKILIEVPEKQREDTVNSLAYEAEARLKDPVYGCIGSIAMLQRKMMELQHDLAIAKERLARCRAVAAAATITTTTTPSRYDFSLLVFPES