AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032113

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032113.t1 MTR_5g039760 44.221 199 84 4 6 202 9 182 4.59e-46 162
MS.gene032113.t1 MTR_0558s0020 34.601 263 140 6 11 247 40 296 1.10e-38 145
MS.gene032113.t1 MTR_6g051425 32.014 278 170 4 9 274 19 289 3.21e-38 145
MS.gene032113.t1 MTR_0402s0040 40.887 203 108 3 4 204 23 215 3.59e-38 149
MS.gene032113.t1 MTR_7g074790 45.029 171 74 3 6 175 9 160 1.32e-37 140
MS.gene032113.t1 MTR_6g090505 35.417 240 129 5 35 254 5 238 9.12e-34 133
MS.gene032113.t1 MTR_8g446020 38.202 178 108 1 72 247 79 256 5.53e-33 128
MS.gene032113.t1 MTR_7g061980 47.826 115 58 2 3 116 6 119 1.93e-28 110
MS.gene032113.t1 MTR_5g015480 47.541 122 62 1 7 126 56 177 3.13e-27 111
MS.gene032113.t1 MTR_4g102720 35.928 167 73 2 6 172 6 138 1.42e-26 108
MS.gene032113.t1 MTR_7g446520 30.085 236 150 2 2 237 83 303 7.38e-26 109
MS.gene032113.t1 MTR_1g083640 29.832 238 135 6 11 222 55 286 2.87e-25 106
MS.gene032113.t1 MTR_1g070355 28.163 245 165 6 11 248 78 318 3.97e-24 107
MS.gene032113.t1 MTR_5g076070 27.273 231 159 5 10 235 56 282 3.06e-21 94.7
MS.gene032113.t1 MTR_7g446510 30.636 173 120 0 83 255 157 329 3.97e-21 95.5
MS.gene032113.t1 MTR_1g057920 44.086 93 50 1 4 94 31 123 9.37e-21 89.0
MS.gene032113.t1 MTR_7g446480 30.636 173 120 0 83 255 142 314 3.90e-20 92.4
MS.gene032113.t1 MTR_2g070590 42.991 107 59 1 4 108 14 120 6.73e-20 86.3
MS.gene032113.t1 MTR_0337s0040 33.566 143 93 1 72 212 80 222 1.91e-19 88.2
MS.gene032113.t1 MTR_6g017200 44.898 98 52 1 4 99 55 152 2.30e-19 85.9
MS.gene032113.t1 MTR_7g113010 40.000 130 62 2 4 131 454 569 5.70e-19 91.7
MS.gene032113.t1 MTR_2g099430 48.235 85 44 0 32 116 28 112 1.40e-17 79.3
MS.gene032113.t1 MTR_4g035820 23.295 352 247 9 4 343 72 412 2.40e-17 86.7
MS.gene032113.t1 MTR_1g086270 34.286 105 62 2 301 398 1 105 8.89e-16 73.9
MS.gene032113.t1 MTR_6g022070 27.168 173 119 3 301 472 1 167 1.30e-15 79.3
MS.gene032113.t1 MTR_4g067290 38.835 103 61 2 6 107 55 156 2.07e-14 73.9
MS.gene032113.t1 MTR_4g067290 38.835 103 61 2 6 107 55 156 2.12e-14 73.6
MS.gene032113.t1 MTR_2g014100 27.778 198 123 4 11 188 48 245 2.38e-14 73.9
MS.gene032113.t1 MTR_4g067290 38.835 103 61 2 6 107 75 176 4.05e-14 73.2
MS.gene032113.t1 MTR_4g067290 38.835 103 61 2 6 107 73 174 4.11e-14 73.2
MS.gene032113.t1 MTR_4g067290 38.835 103 61 2 6 107 75 176 4.25e-14 73.2
MS.gene032113.t1 MTR_4g067290 38.835 103 61 2 6 107 115 216 6.16e-14 73.6
MS.gene032113.t1 MTR_6g022080 28.758 153 104 3 18 166 96 247 2.92e-13 70.9
MS.gene032113.t1 MTR_1g013060 26.570 207 143 5 6 205 23 227 3.98e-13 69.7
MS.gene032113.t1 MTR_2g098880 37.143 105 63 2 6 107 60 164 2.04e-12 68.2
MS.gene032113.t1 MTR_2g098880 37.143 105 63 2 6 107 72 176 2.41e-12 68.2
MS.gene032113.t1 MTR_7g028855 35.075 134 82 3 2 131 115 247 2.59e-12 70.1
MS.gene032113.t1 MTR_2g098920 36.893 103 63 2 6 107 79 180 4.53e-12 67.4
MS.gene032113.t1 MTR_1g055240 33.043 115 75 1 72 184 30 144 5.27e-12 64.7
MS.gene032113.t1 MTR_3g006370 37.500 104 63 2 6 107 49 152 2.20e-11 64.7
MS.gene032113.t1 MTR_1g008290 25.899 139 100 2 65 203 9 144 3.84e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032113.t1 AT4G38180 31.604 636 426 7 3 632 69 701 5.45e-111 353
MS.gene032113.t1 AT4G15090 29.968 624 424 7 3 622 47 661 9.48e-98 320
MS.gene032113.t1 AT4G15090 29.968 624 424 7 3 622 47 661 9.48e-98 320
MS.gene032113.t1 AT4G15090 29.968 624 424 7 3 622 47 661 9.48e-98 320
MS.gene032113.t1 AT4G15090 29.968 624 424 7 3 622 47 661 9.48e-98 320
MS.gene032113.t1 AT4G15090 29.968 624 424 7 3 622 47 661 9.48e-98 320
MS.gene032113.t1 AT3G22170 29.167 624 390 9 2 607 65 654 1.62e-88 295
MS.gene032113.t1 AT3G22170 29.167 624 390 9 2 607 65 654 1.62e-88 295
MS.gene032113.t1 AT1G76320 29.716 599 401 9 11 607 1 581 6.92e-82 273
MS.gene032113.t1 AT1G76320 28.363 684 455 12 11 677 1 666 2.19e-81 273
MS.gene032113.t1 AT1G76320 28.363 684 455 12 11 677 1 666 2.19e-81 273
MS.gene032113.t1 AT1G76320 28.363 684 455 12 11 677 1 666 2.98e-81 273
MS.gene032113.t1 AT2G27110 28.730 630 410 11 5 630 47 641 2.14e-75 259
MS.gene032113.t1 AT2G27110 28.730 630 410 11 5 630 47 641 2.14e-75 259
MS.gene032113.t1 AT3G06250 27.840 625 390 14 6 621 189 761 3.09e-75 258
MS.gene032113.t1 AT3G06250 37.864 103 50 2 6 107 27 116 8.57e-14 75.5
MS.gene032113.t1 AT3G06250 27.840 625 390 14 6 621 189 761 3.09e-75 258
MS.gene032113.t1 AT3G06250 37.864 103 50 2 6 107 27 116 8.57e-14 75.5
MS.gene032113.t1 AT3G06250 27.840 625 390 14 6 621 189 761 3.09e-75 258
MS.gene032113.t1 AT3G06250 37.864 103 50 2 6 107 27 116 8.57e-14 75.5
MS.gene032113.t1 AT5G18960 26.603 624 402 14 6 621 210 785 4.05e-69 241
MS.gene032113.t1 AT5G18960 37.864 103 50 2 6 107 42 131 2.48e-13 73.9
MS.gene032113.t1 AT5G18960 26.603 624 402 14 6 621 210 785 4.05e-69 241
MS.gene032113.t1 AT5G18960 37.864 103 50 2 6 107 42 131 2.48e-13 73.9
MS.gene032113.t1 AT2G27110 28.988 514 342 7 119 630 4 496 2.44e-67 235
MS.gene032113.t1 AT4G19990 26.290 601 385 8 10 593 24 583 3.95e-66 232
MS.gene032113.t1 AT4G19990 26.290 601 385 8 10 593 24 583 3.95e-66 232
MS.gene032113.t1 AT1G52520 27.241 569 385 10 7 563 85 636 4.17e-66 231
MS.gene032113.t1 AT4G19990 26.290 601 385 8 10 593 24 583 4.56e-66 232
MS.gene032113.t1 AT4G19990 26.290 601 385 8 10 593 38 597 5.96e-66 232
MS.gene032113.t1 AT4G19990 26.290 601 385 8 10 593 24 583 1.12e-65 231
MS.gene032113.t1 AT4G19990 26.290 601 385 8 10 593 24 583 1.12e-65 231
MS.gene032113.t1 AT4G38170 31.008 387 260 4 181 565 18 399 7.25e-63 219
MS.gene032113.t1 AT4G19990 26.372 565 358 8 46 593 3 526 3.89e-61 218
MS.gene032113.t1 AT2G32250 25.525 619 401 11 10 622 41 605 7.31e-60 215
MS.gene032113.t1 AT2G32250 25.687 619 400 11 10 622 41 605 1.49e-59 215
MS.gene032113.t1 AT2G32250 25.687 619 400 11 10 622 41 605 1.49e-59 215
MS.gene032113.t1 AT2G32250 25.687 619 400 11 10 622 41 605 1.49e-59 215
MS.gene032113.t1 AT2G32250 25.687 619 400 11 10 622 41 605 1.61e-59 215
MS.gene032113.t1 AT2G32250 25.687 619 400 11 10 622 41 605 1.61e-59 215
MS.gene032113.t1 AT2G32250 25.566 618 400 11 11 622 1 564 6.95e-59 212
MS.gene032113.t1 AT2G32250 25.566 618 400 11 11 622 1 564 8.68e-59 212
MS.gene032113.t1 AT1G80010 26.101 613 414 17 3 591 91 688 5.21e-58 209
MS.gene032113.t1 AT1G10240 26.795 571 395 13 4 562 45 604 1.24e-55 202
MS.gene032113.t1 AT1G10240 26.795 571 395 13 4 562 45 604 1.24e-55 202
MS.gene032113.t1 AT5G28530 24.000 625 411 16 7 597 55 649 7.11e-47 177
MS.gene032113.t1 AT5G28530 24.000 625 407 17 7 597 55 645 5.02e-46 174
MS.gene032113.t1 AT5G28530 24.000 625 407 17 7 597 55 645 5.02e-46 174
MS.gene032113.t1 AT5G28530 22.705 599 358 18 7 580 55 573 1.72e-30 127
MS.gene032113.t1 AT5G28530 22.705 599 354 19 7 580 55 569 1.36e-29 125
MS.gene032113.t1 AT3G59470 39.252 107 63 2 2 107 65 170 2.90e-14 73.6
MS.gene032113.t1 AT3G59470 39.252 107 63 2 2 107 65 170 2.90e-14 73.6
MS.gene032113.t1 AT3G59470 39.252 107 63 2 2 107 77 182 4.04e-14 73.6
MS.gene032113.t1 AT3G07500 37.500 104 64 1 5 107 31 134 7.52e-13 68.9
MS.gene032113.t1 AT2G43280 33.562 146 89 5 6 146 21 163 8.20e-12 65.5

Find 126 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAATCACAAACATATTT+TGG 0.063768 2.2:-14846905 MS.gene032113:CDS
TAGATACGACTTGGCAATTT+TGG 0.122360 2.2:-14847481 MS.gene032113:CDS
CGATGTGTACTAAACAATTT+AGG 0.280754 2.2:+14845787 None:intergenic
TTTGCTAGCAGCTAAGAATA+AGG 0.286355 2.2:+14846086 None:intergenic
TTTCGGTGATGTAGTTTCTT+TGG 0.287406 2.2:-14846850 MS.gene032113:CDS
GAAAGACAAAAGATCCTCTT+TGG 0.289157 2.2:-14847342 MS.gene032113:CDS
GGCTGATGCAAGAATGATAT+TGG 0.289765 2.2:-14846883 MS.gene032113:CDS
TTGATCTATTTGATAACAAT+TGG 0.292503 2.2:-14846428 MS.gene032113:CDS
TGCGAAACCGATTGTTGGTT+TGG 0.298872 2.2:-14847516 MS.gene032113:CDS
CATTCTCTATTTCTCGTAAC+CGG 0.307524 2.2:+14845647 None:intergenic
ATATTGGATTATGGATATTT+CGG 0.311385 2.2:-14846867 MS.gene032113:CDS
ACATGGCATTTGATGCAAAA+TGG 0.313268 2.2:-14846576 MS.gene032113:CDS
CTTTAGCCATTGCTTGGGCT+TGG 0.339011 2.2:+14846640 None:intergenic
ATCGAAATGGCAGAGGAATC+TGG 0.346573 2.2:-14847140 MS.gene032113:CDS
GCAAGAATGATATTGGATTA+TGG 0.358291 2.2:-14846876 MS.gene032113:CDS
GGTGTTATTATTGATCATAT+TGG 0.368709 2.2:-14845829 MS.gene032113:CDS
AAAGGATTGTGCCTCTATAA+TGG 0.369850 2.2:+14846769 None:intergenic
AGACGGAATGAAATTCTTAC+TGG 0.375408 2.2:+14847284 None:intergenic
GGCTTAATCTTAAACCCCTT+TGG 0.375508 2.2:+14845574 None:intergenic
TATGGTAAGAAATGTGGTTT+TGG 0.376185 2.2:-14847452 MS.gene032113:CDS
TCTACATAAGCTTGTAGAAA+AGG 0.378913 2.2:-14845704 MS.gene032113:CDS
TTGAACAAACGAAAGGTAGA+TGG 0.405470 2.2:-14847413 MS.gene032113:CDS
CTAGTGCTTTAGCCATTGCT+TGG 0.405838 2.2:+14846634 None:intergenic
TCTCATACAAGCCTTAAAAC+TGG 0.407408 2.2:+14846302 None:intergenic
TAGTGCTTTAGCCATTGCTT+GGG 0.410561 2.2:+14846635 None:intergenic
ATAGTAGTGCAGCCCCAAAA+AGG 0.420352 2.2:+14846751 None:intergenic
ATGCTCCACTATGATTAGAT+TGG 0.422356 2.2:-14845752 MS.gene032113:CDS
ACCTAGGTTGCTTGATGAAA+GGG 0.423781 2.2:-14846545 MS.gene032113:CDS
TATTGATCATATTGGAAATG+AGG 0.429689 2.2:-14845821 MS.gene032113:CDS
CATAACCCATGTCGAGTCTC+TGG 0.431181 2.2:+14846600 None:intergenic
TCAGGCTTTAACCATTATAG+AGG 0.434996 2.2:-14846780 MS.gene032113:CDS
CTACATAAGCTTGTAGAAAA+GGG 0.435662 2.2:-14845703 MS.gene032113:CDS
TGATCTATTTGATAACAATT+GGG 0.442178 2.2:-14846427 MS.gene032113:CDS
CATGATATAGAGAAAGTTGT+TGG 0.444784 2.2:+14845979 None:intergenic
AAGGGGTTTAAGATTAAGCC+TGG 0.452760 2.2:-14845571 MS.gene032113:CDS
TTTCAACCTACTGCCCTCCT+TGG 0.458502 2.2:+14847361 None:intergenic
GACAACGTAGCATGGTGTAT+GGG 0.459098 2.2:-14847013 MS.gene032113:CDS
CCTAGGGCTGATACGAGATC+AGG 0.459959 2.2:-14847311 MS.gene032113:CDS
CATGGCATTTGATGCAAAAT+GGG 0.461652 2.2:-14846575 MS.gene032113:CDS
TTTCTGTGTGTTGTAAAAGA+TGG 0.462097 2.2:+14847216 None:intergenic
AGACAACGTAGCATGGTGTA+TGG 0.465057 2.2:-14847014 MS.gene032113:CDS
TGTTATCACCATGTTTAAAG+TGG 0.466829 2.2:-14846028 MS.gene032113:CDS
CAACAACTTTCTCAAAGTGT+TGG 0.474048 2.2:+14846253 None:intergenic
CGGCAGATTTGTTTGTTCCA+AGG 0.478536 2.2:-14847378 MS.gene032113:CDS
GTGTTGTAAAAGATGGTTGT+GGG 0.481849 2.2:+14847223 None:intergenic
ATTTCATTCCGTCTAAGAGA+TGG 0.482262 2.2:-14847275 MS.gene032113:CDS
CTCTGCCATTTCGATCTCAT+AGG 0.485355 2.2:+14847148 None:intergenic
TTGGGCTTGGTCAGTGAACA+AGG 0.489076 2.2:+14846653 None:intergenic
GAATGTTGATATCTTATCCT+TGG 0.489846 2.2:+14846177 None:intergenic
TGGTCAGTGAACAAGGTTTG+AGG 0.493511 2.2:+14846660 None:intergenic
AATTGGGTGAAATCAATGTA+TGG 0.495445 2.2:-14846411 MS.gene032113:CDS
CTTAATGCGAAACCGATTGT+TGG 0.498271 2.2:-14847521 MS.gene032113:CDS
TGTGCGAGTTGCATTAGAAT+CGG 0.498318 2.2:+14846153 None:intergenic
TCAAATTCCAAACCAACAAT+CGG 0.498729 2.2:+14847509 None:intergenic
ATCAATAATAACACCATCAC+GGG 0.502589 2.2:+14845837 None:intergenic
GTAGATGGCTTGATTACATC+CGG 0.508373 2.2:-14847398 MS.gene032113:CDS
GAATATGAGTCACAACCAAA+GGG 0.510834 2.2:-14845589 MS.gene032113:CDS
ATGGTGATAACATATTCAAA+TGG 0.513168 2.2:+14846039 None:intergenic
ATCACCATGTTTAAAGTGGA+AGG 0.514926 2.2:-14846024 MS.gene032113:CDS
CTCGACATGGGTTATGTACA+TGG 0.515437 2.2:-14846593 MS.gene032113:CDS
CACCTAGGTTGCTTGATGAA+AGG 0.515698 2.2:-14846546 MS.gene032113:CDS
ATGATGGATTGTCCAACATC+TGG 0.516109 2.2:+14846958 None:intergenic
GTATGTAATACATGAGTTTG+AGG 0.516337 2.2:-14847249 MS.gene032113:CDS
CTCTCCTTCCACTTTAAACA+TGG 0.523398 2.2:+14846020 None:intergenic
AAACCGCTGACTCATTCAAA+TGG 0.528647 2.2:-14846722 MS.gene032113:CDS
AGGTGAAGTTTGAAGCAGCT+TGG 0.529588 2.2:-14846470 MS.gene032113:CDS
TGATATGCGTGGTAAAATGA+TGG 0.529588 2.2:+14846942 None:intergenic
TTTGTTCCAAGGAGGGCAGT+AGG 0.531121 2.2:-14847367 MS.gene032113:CDS
TTTCATTCCGTCTAAGAGAT+GGG 0.532627 2.2:-14847274 MS.gene032113:CDS
AGCATGGTGTATGGGGAAGT+TGG 0.534278 2.2:-14847005 MS.gene032113:CDS
TTTGATCTTTGACGTGGTGT+AGG 0.536468 2.2:+14845472 None:intergenic
TAATCTCGGATTTACACGAT+TGG 0.536812 2.2:-14847063 MS.gene032113:CDS
CATACTTCCCATCTCTTAGA+CGG 0.539409 2.2:+14847267 None:intergenic
CTTGGAACAAACAAATCTGC+CGG 0.541110 2.2:+14847379 None:intergenic
AGCTGCCAATCTAATCATAG+TGG 0.546809 2.2:+14845747 None:intergenic
ATTCTCTATTTCTCGTAACC+GGG 0.548252 2.2:+14845648 None:intergenic
TGAAGCAGCTTGGAGCAAGT+TGG 0.550033 2.2:-14846460 MS.gene032113:CDS
AGAATATGAGTCACAACCAA+AGG 0.553725 2.2:-14845590 MS.gene032113:CDS
TCAAGCCTATGAGATCGAAA+TGG 0.558373 2.2:-14847153 MS.gene032113:CDS
TTTGTATGACTTGTGTAAGC+TGG 0.560502 2.2:-14845680 MS.gene032113:CDS
ACACTTTGAGAAAGTTGTTG+AGG 0.561537 2.2:-14846250 MS.gene032113:CDS
CCTGATCTCGTATCAGCCCT+AGG 0.564208 2.2:+14847311 None:intergenic
AGATTTGTTTGTTCCAAGGA+GGG 0.566275 2.2:-14847374 MS.gene032113:CDS
CCCCTTTCATCAAGCAACCT+AGG 0.566470 2.2:+14846544 None:intergenic
TGTGTTGTAAAAGATGGTTG+TGG 0.567063 2.2:+14847222 None:intergenic
ATGAACAAAGCTATCACACT+TGG 0.569240 2.2:-14846360 MS.gene032113:CDS
GTTGAATATGGTAAGAAATG+TGG 0.571393 2.2:-14847458 MS.gene032113:CDS
CAGATTTGTTTGTTCCAAGG+AGG 0.577739 2.2:-14847375 MS.gene032113:CDS
TCATGCATTAAAAGTCATTG+AGG 0.578104 2.2:-14845917 MS.gene032113:CDS
TTGATAGCGTAGATACGACT+TGG 0.578993 2.2:-14847490 MS.gene032113:CDS
TGTTTAAAGTGGAAGGAGAG+TGG 0.579070 2.2:-14846017 MS.gene032113:CDS
CACAGTCCATTTGATATGCG+TGG 0.579117 2.2:+14846931 None:intergenic
AGCAAGAAGACAACGTAGCA+TGG 0.582684 2.2:-14847021 MS.gene032113:CDS
TTTAAGGCTTGTATGAGACC+TGG 0.585363 2.2:-14846297 MS.gene032113:CDS
GCAGGAGGTAGAACTAATCT+CGG 0.585710 2.2:-14847077 MS.gene032113:CDS
CATGTATGAATACGATCAAG+AGG 0.589868 2.2:-14846490 MS.gene032113:CDS
CAACTTATGAGCACACAGGC+AGG 0.592345 2.2:-14847095 MS.gene032113:CDS
AAGCCTGAAAATAATGCAAG+TGG 0.596112 2.2:+14846795 None:intergenic
TGACTTGTGTAAGCTGGTGA+TGG 0.596476 2.2:-14845674 MS.gene032113:CDS
CACTGACCAAGCCCAAGCAA+TGG 0.599270 2.2:-14846646 MS.gene032113:CDS
ACAACGTAGCATGGTGTATG+GGG 0.603463 2.2:-14847012 MS.gene032113:CDS
GATCAATAATAACACCATCA+CGG 0.609274 2.2:+14845836 None:intergenic
TACACCTTTGATCTTTGACG+TGG 0.609768 2.2:+14845466 None:intergenic
ATAGAGAATGCTGATGATGA+AGG 0.609789 2.2:-14845634 MS.gene032113:CDS
TAACCATTTGAATGAGTCAG+CGG 0.612529 2.2:+14846719 None:intergenic
GAAACATTTCTTGAAGCACA+TGG 0.613854 2.2:-14846693 MS.gene032113:CDS
AAGTGGTCGATTAGCACGGT+TGG 0.614021 2.2:+14846812 None:intergenic
CTATGAGATCGAAATGGCAG+AGG 0.614810 2.2:-14847147 MS.gene032113:CDS
CCTAGGTTGCTTGATGAAAG+GGG 0.618212 2.2:-14846544 MS.gene032113:CDS
ATGCAAGTGGTCGATTAGCA+CGG 0.619115 2.2:+14846808 None:intergenic
GTAAGCTGGTGATGGAGGCC+CGG 0.626121 2.2:-14845666 MS.gene032113:CDS
TACACCACGTCAAAGATCAA+AGG 0.627890 2.2:-14845470 MS.gene032113:CDS
CTAGGGCTGATACGAGATCA+GGG 0.628499 2.2:-14847310 MS.gene032113:CDS
CTTGTGTAAGCTGGTGATGG+AGG 0.636254 2.2:-14845671 MS.gene032113:CDS
GTGATGCCAGAGACTCGACA+TGG 0.645053 2.2:-14846606 MS.gene032113:CDS
CAAAATGGGATCAAACACCT+AGG 0.665706 2.2:-14846561 MS.gene032113:CDS
TGATTACTTGAACAAACGAA+AGG 0.666107 2.2:-14847420 MS.gene032113:CDS
CTAGGTTGCTTGATGAAAGG+GGG 0.671384 2.2:-14846543 MS.gene032113:CDS
AATTGAATAACACTGATACC+AGG 0.672015 2.2:+14846279 None:intergenic
ATTTCAACTTATGAGCACAC+AGG 0.673642 2.2:-14847099 MS.gene032113:CDS
AATGGCTAAAGCACTAGTTG+AGG 0.676750 2.2:-14846628 MS.gene032113:CDS
TGGACAAGAGATGCCCGTGA+TGG 0.687751 2.2:-14845850 MS.gene032113:CDS
CTTATGAGCACACAGGCAGG+AGG 0.695932 2.2:-14847092 MS.gene032113:CDS
AGTCTCGACATAAACTACCA+AGG 0.709308 2.2:-14846194 MS.gene032113:CDS
AATATGAGTCACAACCAAAG+GGG 0.711774 2.2:-14845588 MS.gene032113:CDS
TGATGCCAGAGACTCGACAT+GGG 0.714570 2.2:-14846605 MS.gene032113:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTGGATTATGGATATTT+CGG - chr2.2:14846125-14846144 MS.gene032113:CDS 20.0%
!! ATCAATACATCTTAAAAAGA+TGG - chr2.2:14847122-14847141 MS.gene032113:CDS 20.0%
!!! AACAAATCACAAACATATTT+TGG - chr2.2:14846087-14846106 MS.gene032113:CDS 20.0%
!!! ACAAATCACAAACATATTTT+GGG - chr2.2:14846088-14846107 MS.gene032113:CDS 20.0%
!!! CTTTTTAAGATGTATTGATT+GGG + chr2.2:14847121-14847140 None:intergenic 20.0%
!!! TCTTTTTAAGATGTATTGAT+TGG + chr2.2:14847122-14847141 None:intergenic 20.0%
!!! TGATCTATTTGATAACAATT+GGG - chr2.2:14846565-14846584 MS.gene032113:CDS 20.0%
!!! TGTAAAAAATTTGAGACTTT+TGG - chr2.2:14847040-14847059 MS.gene032113:CDS 20.0%
!!! TTGATCTATTTGATAACAAT+TGG - chr2.2:14846564-14846583 MS.gene032113:CDS 20.0%
!!! TTTTTAAGATGTATTGATTG+GGG + chr2.2:14847120-14847139 None:intergenic 20.0%
! ATGGTGATAACATATTCAAA+TGG + chr2.2:14846956-14846975 None:intergenic 25.0%
! TATTGATCATATTGGAAATG+AGG - chr2.2:14847171-14847190 MS.gene032113:CDS 25.0%
!! ATATTCTTTTGTTACTGTTG+GGG + chr2.2:14847389-14847408 None:intergenic 25.0%
!! CATATTCTTTTGTTACTGTT+GGG + chr2.2:14847390-14847409 None:intergenic 25.0%
!! GAGTTTAAATTCCAGTTTTA+AGG - chr2.2:14846679-14846698 MS.gene032113:CDS 25.0%
!! TCATATTCTTTTGTTACTGT+TGG + chr2.2:14847391-14847410 None:intergenic 25.0%
!!! GGTGTTATTATTGATCATAT+TGG - chr2.2:14847163-14847182 MS.gene032113:CDS 25.0%
AATTGAATAACACTGATACC+AGG + chr2.2:14846716-14846735 None:intergenic 30.0%
AATTGGGTGAAATCAATGTA+TGG - chr2.2:14846581-14846600 MS.gene032113:CDS 30.0%
ATCAATAATAACACCATCAC+GGG + chr2.2:14847158-14847177 None:intergenic 30.0%
CATGATATAGAGAAAGTTGT+TGG + chr2.2:14847016-14847035 None:intergenic 30.0%
CGATGTGTACTAAACAATTT+AGG + chr2.2:14847208-14847227 None:intergenic 30.0%
CTACATAAGCTTGTAGAAAA+GGG - chr2.2:14847289-14847308 MS.gene032113:CDS 30.0%
GAATGTTGATATCTTATCCT+TGG + chr2.2:14846818-14846837 None:intergenic 30.0%
GATCAATAATAACACCATCA+CGG + chr2.2:14847159-14847178 None:intergenic 30.0%
GCAAGAATGATATTGGATTA+TGG - chr2.2:14846116-14846135 MS.gene032113:CDS 30.0%
GTATGGTATCAAGCAAAAAT+GGG - chr2.2:14846598-14846617 MS.gene032113:CDS 30.0%
GTATGTAATACATGAGTTTG+AGG - chr2.2:14845743-14845762 MS.gene032113:CDS 30.0%
GTTGAATATGGTAAGAAATG+TGG - chr2.2:14845534-14845553 MS.gene032113:CDS 30.0%
TCAAATTCCAAACCAACAAT+CGG + chr2.2:14845486-14845505 None:intergenic 30.0%
TCATGCATTAAAAGTCATTG+AGG - chr2.2:14847075-14847094 MS.gene032113:CDS 30.0%
TCTACATAAGCTTGTAGAAA+AGG - chr2.2:14847288-14847307 MS.gene032113:CDS 30.0%
TGATTACTTGAACAAACGAA+AGG - chr2.2:14845572-14845591 MS.gene032113:CDS 30.0%
TGTATGGTATCAAGCAAAAA+TGG - chr2.2:14846597-14846616 MS.gene032113:CDS 30.0%
TGTTATCACCATGTTTAAAG+TGG - chr2.2:14846964-14846983 MS.gene032113:CDS 30.0%
TTTCTGTGTGTTGTAAAAGA+TGG + chr2.2:14845779-14845798 None:intergenic 30.0%
! ATTTTACCACGCATATCAAA+TGG - chr2.2:14846055-14846074 MS.gene032113:CDS 30.0%
! TATGGTAAGAAATGTGGTTT+TGG - chr2.2:14845540-14845559 MS.gene032113:CDS 30.0%
AAAGGATTGTGCCTCTATAA+TGG + chr2.2:14846226-14846245 None:intergenic 35.0%
AAGCCTGAAAATAATGCAAG+TGG + chr2.2:14846200-14846219 None:intergenic 35.0%
AATATGAGTCACAACCAAAG+GGG - chr2.2:14847404-14847423 MS.gene032113:CDS 35.0%
ACATGGCATTTGATGCAAAA+TGG - chr2.2:14846416-14846435 MS.gene032113:CDS 35.0%
AGAATATGAGTCACAACCAA+AGG - chr2.2:14847402-14847421 MS.gene032113:CDS 35.0%
AGACGGAATGAAATTCTTAC+TGG + chr2.2:14845711-14845730 None:intergenic 35.0%
ATGAACAAAGCTATCACACT+TGG - chr2.2:14846632-14846651 MS.gene032113:CDS 35.0%
ATGCTCCACTATGATTAGAT+TGG - chr2.2:14847240-14847259 MS.gene032113:CDS 35.0%
ATTTCAACTTATGAGCACAC+AGG - chr2.2:14845893-14845912 MS.gene032113:CDS 35.0%
ATTTCATTCCGTCTAAGAGA+TGG - chr2.2:14845717-14845736 MS.gene032113:CDS 35.0%
CAACAACTTTCTCAAAGTGT+TGG + chr2.2:14846742-14846761 None:intergenic 35.0%
CATGGCATTTGATGCAAAAT+GGG - chr2.2:14846417-14846436 MS.gene032113:CDS 35.0%
CATGTATGAATACGATCAAG+AGG - chr2.2:14846502-14846521 MS.gene032113:CDS 35.0%
CTCTTTGGTTAAAAAGCCTA+GGG - chr2.2:14845665-14845684 MS.gene032113:CDS 35.0%
GAAACATTTCTTGAAGCACA+TGG - chr2.2:14846299-14846318 MS.gene032113:CDS 35.0%
GAAAGACAAAAGATCCTCTT+TGG - chr2.2:14845650-14845669 MS.gene032113:CDS 35.0%
GAATATGAGTCACAACCAAA+GGG - chr2.2:14847403-14847422 MS.gene032113:CDS 35.0%
GTGTTGTAAAAGATGGTTGT+GGG + chr2.2:14845772-14845791 None:intergenic 35.0%
TAACCATTTGAATGAGTCAG+CGG + chr2.2:14846276-14846295 None:intergenic 35.0%
TAATCTCGGATTTACACGAT+TGG - chr2.2:14845929-14845948 MS.gene032113:CDS 35.0%
TCTCATACAAGCCTTAAAAC+TGG + chr2.2:14846693-14846712 None:intergenic 35.0%
TGATATGCGTGGTAAAATGA+TGG + chr2.2:14846053-14846072 None:intergenic 35.0%
TGTGTTGTAAAAGATGGTTG+TGG + chr2.2:14845773-14845792 None:intergenic 35.0%
TTGAACAAACGAAAGGTAGA+TGG - chr2.2:14845579-14845598 MS.gene032113:CDS 35.0%
TTTCATTCCGTCTAAGAGAT+GGG - chr2.2:14845718-14845737 MS.gene032113:CDS 35.0%
TTTCGGTGATGTAGTTTCTT+TGG - chr2.2:14846142-14846161 MS.gene032113:CDS 35.0%
TTTGCTAGCAGCTAAGAATA+AGG + chr2.2:14846909-14846928 None:intergenic 35.0%
! ACACTTTGAGAAAGTTGTTG+AGG - chr2.2:14846742-14846761 MS.gene032113:CDS 35.0%
! AGATTTGTTTGTTCCAAGGA+GGG - chr2.2:14845618-14845637 MS.gene032113:CDS 35.0%
! ATAGAGAATGCTGATGATGA+AGG - chr2.2:14847358-14847377 MS.gene032113:CDS 35.0%
! ATCACCATGTTTAAAGTGGA+AGG - chr2.2:14846968-14846987 MS.gene032113:CDS 35.0%
! ATTCTCTATTTCTCGTAACC+GGG + chr2.2:14847347-14847366 None:intergenic 35.0%
! CATTCTCTATTTCTCGTAAC+CGG + chr2.2:14847348-14847367 None:intergenic 35.0%
! GTATTTTCAACACCAGATGT+TGG - chr2.2:14846022-14846041 MS.gene032113:CDS 35.0%
! TCAGGCTTTAACCATTATAG+AGG - chr2.2:14846212-14846231 MS.gene032113:CDS 35.0%
! TTTGTATGACTTGTGTAAGC+TGG - chr2.2:14847312-14847331 MS.gene032113:CDS 35.0%
!! AGATACGACTTGGCAATTTT+GGG - chr2.2:14845512-14845531 MS.gene032113:CDS 35.0%
!! ATAGAGGCACAATCCTTTTT+GGG - chr2.2:14846228-14846247 MS.gene032113:CDS 35.0%
!! TAGATACGACTTGGCAATTT+TGG - chr2.2:14845511-14845530 MS.gene032113:CDS 35.0%
!! TATAGAGGCACAATCCTTTT+TGG - chr2.2:14846227-14846246 MS.gene032113:CDS 35.0%
!!! TGGCAATTTTGGGTTGAATA+TGG - chr2.2:14845522-14845541 MS.gene032113:CDS 35.0%
ACCTAGGTTGCTTGATGAAA+GGG - chr2.2:14846447-14846466 MS.gene032113:CDS 40.0%
AGCTGCCAATCTAATCATAG+TGG + chr2.2:14847248-14847267 None:intergenic 40.0%
AGTCTCGACATAAACTACCA+AGG - chr2.2:14846798-14846817 MS.gene032113:CDS 40.0%
CAAAATGGGATCAAACACCT+AGG - chr2.2:14846431-14846450 MS.gene032113:CDS 40.0%
CATACTTCCCATCTCTTAGA+CGG + chr2.2:14845728-14845747 None:intergenic 40.0%
CCTCTTTGGTTAAAAAGCCT+AGG - chr2.2:14845664-14845683 MS.gene032113:CDS 40.0%
CTCTCCTTCCACTTTAAACA+TGG + chr2.2:14846975-14846994 None:intergenic 40.0%
CTTAATGCGAAACCGATTGT+TGG - chr2.2:14845471-14845490 MS.gene032113:CDS 40.0%
CTTGGAACAAACAAATCTGC+CGG + chr2.2:14845616-14845635 None:intergenic 40.0%
GGCTGATGCAAGAATGATAT+TGG - chr2.2:14846109-14846128 MS.gene032113:CDS 40.0%
GGCTTAATCTTAAACCCCTT+TGG + chr2.2:14847421-14847440 None:intergenic 40.0%
GTAGATGGCTTGATTACATC+CGG - chr2.2:14845594-14845613 MS.gene032113:CDS 40.0%
TACACCACGTCAAAGATCAA+AGG - chr2.2:14847522-14847541 MS.gene032113:CDS 40.0%
TACACCTTTGATCTTTGACG+TGG + chr2.2:14847529-14847548 None:intergenic 40.0%
TAGTGCTTTAGCCATTGCTT+GGG + chr2.2:14846360-14846379 None:intergenic 40.0%
TCAAGCCTATGAGATCGAAA+TGG - chr2.2:14845839-14845858 MS.gene032113:CDS 40.0%
TGTGCGAGTTGCATTAGAAT+CGG + chr2.2:14846842-14846861 None:intergenic 40.0%
TTTGATCTTTGACGTGGTGT+AGG + chr2.2:14847523-14847542 None:intergenic 40.0%
! AAGGGGTTTAAGATTAAGCC+TGG - chr2.2:14847421-14847440 MS.gene032113:CDS 40.0%
! AATGGCTAAAGCACTAGTTG+AGG - chr2.2:14846364-14846383 MS.gene032113:CDS 40.0%
! CAGATTTGTTTGTTCCAAGG+AGG - chr2.2:14845617-14845636 MS.gene032113:CDS 40.0%
! CCTAGGCTTTTTAACCAAAG+AGG + chr2.2:14845667-14845686 None:intergenic 40.0%
! CGACCACTTGCATTATTTTC+AGG - chr2.2:14846194-14846213 MS.gene032113:CDS 40.0%
! CGTCTACGATTTTGTAATCC+AGG + chr2.2:14847442-14847461 None:intergenic 40.0%
! TGTTTAAAGTGGAAGGAGAG+TGG - chr2.2:14846975-14846994 MS.gene032113:CDS 40.0%
! TTTAAGGCTTGTATGAGACC+TGG - chr2.2:14846695-14846714 MS.gene032113:CDS 40.0%
!! AAACCGCTGACTCATTCAAA+TGG - chr2.2:14846270-14846289 MS.gene032113:CDS 40.0%
!! ATGATGGATTGTCCAACATC+TGG + chr2.2:14846037-14846056 None:intergenic 40.0%
!! TAGAGGCACAATCCTTTTTG+GGG - chr2.2:14846229-14846248 MS.gene032113:CDS 40.0%
!! TTGATAGCGTAGATACGACT+TGG - chr2.2:14845502-14845521 MS.gene032113:CDS 40.0%
ACAACGTAGCATGGTGTATG+GGG - chr2.2:14845980-14845999 MS.gene032113:CDS 45.0%
AGACAACGTAGCATGGTGTA+TGG - chr2.2:14845978-14845997 MS.gene032113:CDS 45.0%
AGCAAGAAGACAACGTAGCA+TGG - chr2.2:14845971-14845990 MS.gene032113:CDS 45.0%
ATAGTAGTGCAGCCCCAAAA+AGG + chr2.2:14846244-14846263 None:intergenic 45.0%
ATCGAAATGGCAGAGGAATC+TGG - chr2.2:14845852-14845871 MS.gene032113:CDS 45.0%
ATGCAAGTGGTCGATTAGCA+CGG + chr2.2:14846187-14846206 None:intergenic 45.0%
CACAGTCCATTTGATATGCG+TGG + chr2.2:14846064-14846083 None:intergenic 45.0%
CACCTAGGTTGCTTGATGAA+AGG - chr2.2:14846446-14846465 MS.gene032113:CDS 45.0%
CCTAGGTTGCTTGATGAAAG+GGG - chr2.2:14846448-14846467 MS.gene032113:CDS 45.0%
CTAGGTTGCTTGATGAAAGG+GGG - chr2.2:14846449-14846468 MS.gene032113:CDS 45.0%
CTAGTGCTTTAGCCATTGCT+TGG + chr2.2:14846361-14846380 None:intergenic 45.0%
CTATGAGATCGAAATGGCAG+AGG - chr2.2:14845845-14845864 MS.gene032113:CDS 45.0%
GACAACGTAGCATGGTGTAT+GGG - chr2.2:14845979-14845998 MS.gene032113:CDS 45.0%
GCAGGAGGTAGAACTAATCT+CGG - chr2.2:14845915-14845934 MS.gene032113:CDS 45.0%
TGGTCAGTGAACAAGGTTTG+AGG + chr2.2:14846335-14846354 None:intergenic 45.0%
! AGGTGAAGTTTGAAGCAGCT+TGG - chr2.2:14846522-14846541 MS.gene032113:CDS 45.0%
! CGGCAGATTTGTTTGTTCCA+AGG - chr2.2:14845614-14845633 MS.gene032113:CDS 45.0%
! CTCGACATGGGTTATGTACA+TGG - chr2.2:14846399-14846418 MS.gene032113:CDS 45.0%
! CTCTGCCATTTCGATCTCAT+AGG + chr2.2:14845847-14845866 None:intergenic 45.0%
! TGACTTGTGTAAGCTGGTGA+TGG - chr2.2:14847318-14847337 MS.gene032113:CDS 45.0%
! TGCGAAACCGATTGTTGGTT+TGG - chr2.2:14845476-14845495 MS.gene032113:CDS 45.0%
AAGTGGTCGATTAGCACGGT+TGG + chr2.2:14846183-14846202 None:intergenic 50.0%
AGCATGGTGTATGGGGAAGT+TGG - chr2.2:14845987-14846006 MS.gene032113:CDS 50.0%
CAACTTATGAGCACACAGGC+AGG - chr2.2:14845897-14845916 MS.gene032113:CDS 50.0%
CATAACCCATGTCGAGTCTC+TGG + chr2.2:14846395-14846414 None:intergenic 50.0%
CCCCTTTCATCAAGCAACCT+AGG + chr2.2:14846451-14846470 None:intergenic 50.0%
CTAGGGCTGATACGAGATCA+GGG - chr2.2:14845682-14845701 MS.gene032113:CDS 50.0%
CTTTAGCCATTGCTTGGGCT+TGG + chr2.2:14846355-14846374 None:intergenic 50.0%
TGAAGCAGCTTGGAGCAAGT+TGG - chr2.2:14846532-14846551 MS.gene032113:CDS 50.0%
TGATGCCAGAGACTCGACAT+GGG - chr2.2:14846387-14846406 MS.gene032113:CDS 50.0%
TTTCAACCTACTGCCCTCCT+TGG + chr2.2:14845634-14845653 None:intergenic 50.0%
TTTGTTCCAAGGAGGGCAGT+AGG - chr2.2:14845625-14845644 MS.gene032113:CDS 50.0%
! TTGGGCTTGGTCAGTGAACA+AGG + chr2.2:14846342-14846361 None:intergenic 50.0%
!! CTTGTGTAAGCTGGTGATGG+AGG - chr2.2:14847321-14847340 MS.gene032113:CDS 50.0%
CACTGACCAAGCCCAAGCAA+TGG - chr2.2:14846346-14846365 MS.gene032113:CDS 55.0%
CCTAGGGCTGATACGAGATC+AGG - chr2.2:14845681-14845700 MS.gene032113:CDS 55.0%
CCTGATCTCGTATCAGCCCT+AGG + chr2.2:14845684-14845703 None:intergenic 55.0%
CTTATGAGCACACAGGCAGG+AGG - chr2.2:14845900-14845919 MS.gene032113:CDS 55.0%
GTGATGCCAGAGACTCGACA+TGG - chr2.2:14846386-14846405 MS.gene032113:CDS 55.0%
TGGACAAGAGATGCCCGTGA+TGG - chr2.2:14847142-14847161 MS.gene032113:CDS 55.0%
!! GTAAGCTGGTGATGGAGGCC+CGG - chr2.2:14847326-14847345 MS.gene032113:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 14845465 14847549 14845465 ID=MS.gene032113
chr2.2 mRNA 14845465 14847549 14845465 ID=MS.gene032113.t1;Parent=MS.gene032113
chr2.2 exon 14845465 14847549 14845465 ID=MS.gene032113.t1.exon1;Parent=MS.gene032113.t1
chr2.2 CDS 14845465 14847549 14845465 ID=cds.MS.gene032113.t1;Parent=MS.gene032113.t1
Gene Sequence

>MS.gene032113

ATGGCACTTAATGCGAAACCGATTGTTGGTTTGGAATTTGATAGCGTAGATACGACTTGGCAATTTTGGGTTGAATATGGTAAGAAATGTGGTTTTGGAGTTAGAAATGATTACTTGAACAAACGAAAGGTAGATGGCTTGATTACATCCGGCAGATTTGTTTGTTCCAAGGAGGGCAGTAGGTTGAAAGACAAAAGATCCTCTTTGGTTAAAAAGCCTAGGGCTGATACGAGATCAGGGTGTCCAGTAAGAATTTCATTCCGTCTAAGAGATGGGAAGTATGTAATACATGAGTTTGAGGAAACCCACAACCATCTTTTACAACACACAGAAACAACACACATGCTAGCATCACATCGTAAAATATCAGAAGTTCAAGCCTATGAGATCGAAATGGCAGAGGAATCTGGACTAATGCAAAAGTCTTCATTTCAACTTATGAGCACACAGGCAGGAGGTAGAACTAATCTCGGATTTACACGATTGGATGTCAAAAACTATCTTAAAGCAAGAAGACAACGTAGCATGGTGTATGGGGAAGTTGGTAGTCTTATGCAGTATTTTCAACACCAGATGTTGGACAATCCATCATTTTACCACGCATATCAAATGGACTGTGATGAACAAATCACAAACATATTTTGGGCTGATGCAAGAATGATATTGGATTATGGATATTTCGGTGATGTAGTTTCTTTGGATACAACATATTGCACCAACCGTGCTAATCGACCACTTGCATTATTTTCAGGCTTTAACCATTATAGAGGCACAATCCTTTTTGGGGCTGCACTACTATATGATGAAACCGCTGACTCATTCAAATGGTTATTTGAAACATTTCTTGAAGCACATGGAAATAAAAAGCCTCAAACCTTGTTCACTGACCAAGCCCAAGCAATGGCTAAAGCACTAGTTGAGGTGATGCCAGAGACTCGACATGGGTTATGTACATGGCATTTGATGCAAAATGGGATCAAACACCTAGGTTGCTTGATGAAAGGGGGAAGTGATTTACTATCTGACTTTAAGAAGTGCATGTATGAATACGATCAAGAGGTGAAGTTTGAAGCAGCTTGGAGCAAGTTGGTTGCAAAATTTGATCTATTTGATAACAATTGGGTGAAATCAATGTATGGTATCAAGCAAAAATGGGCATCATGTCACATGAACAAAGCTATCACACTTGGAATGCGAAGCACTCAACTTAGTGAGAGTTTAAATTCCAGTTTTAAGGCTTGTATGAGACCTGGTATCAGTGTTATTCAATTCTTCCAACACTTTGAGAAAGTTGTTGAGGAGAAACGATACAACGAGTTAGTATGTGAGTATGAGTCTCGACATAAACTACCAAGGATAAGATATCAACATTCTCCGATTCTAATGCAACTCGCACAAGTGTACACACATAAAATCTTTGATATGTTCAATGATGAGTTTTCCTTATTCTTAGCTGCTAGCAAAAAAAGTGAGAATCTGTCTCAACTTCCATTTGAATATGTTATCACCATGTTTAAAGTGGAAGGAGAGTGGACAGTACTATTTGATCCAACAACTTTCTCTATATCATGTACTTGTAAAAAATTTGAGACTTTTGGCATACTCTGTTGTCATGCATTAAAAGTCATTGAGGCAAATGATGTTAAACTGATCCCCAATCAATACATCTTAAAAAGATGGACAAGAGATGCCCGTGATGGTGTTATTATTGATCATATTGGAAATGAGGTTGTTGATGATCCTAAATTGTTTAGTACACATCGATATAAGAGATTATGCTCCACTATGATTAGATTGGCAGCTGACGTGTCAACATCTGAATATCTACATAAGCTTGTAGAAAAGGGTTTGTATGACTTGTGTAAGCTGGTGATGGAGGCCCGGTTACGAGAAATAGAGAATGCTGATGATGAAGGTGATGCCCCAACAGTAACAAAAGAATATGAGTCACAACCAAAGGGGTTTAAGATTAAGCCTGGATTACAAAATCGTAGACGACTAAAGAGTTGTGTCGAGCTTATGTCTAAGAAAAAAAGCAAAGCAACAATTGTAAAACCTACACCACGTCAAAGATCAAAGGTGTAA
Protein sequence

>MS.gene032113.t1

MALNAKPIVGLEFDSVDTTWQFWVEYGKKCGFGVRNDYLNKRKVDGLITSGRFVCSKEGSRLKDKRSSLVKKPRADTRSGCPVRISFRLRDGKYVIHEFEETHNHLLQHTETTHMLASHRKISEVQAYEIEMAEESGLMQKSSFQLMSTQAGGRTNLGFTRLDVKNYLKARRQRSMVYGEVGSLMQYFQHQMLDNPSFYHAYQMDCDEQITNIFWADARMILDYGYFGDVVSLDTTYCTNRANRPLALFSGFNHYRGTILFGAALLYDETADSFKWLFETFLEAHGNKKPQTLFTDQAQAMAKALVEVMPETRHGLCTWHLMQNGIKHLGCLMKGGSDLLSDFKKCMYEYDQEVKFEAAWSKLVAKFDLFDNNWVKSMYGIKQKWASCHMNKAITLGMRSTQLSESLNSSFKACMRPGISVIQFFQHFEKVVEEKRYNELVCEYESRHKLPRIRYQHSPILMQLAQVYTHKIFDMFNDEFSLFLAASKKSENLSQLPFEYVITMFKVEGEWTVLFDPTTFSISCTCKKFETFGILCCHALKVIEANDVKLIPNQYILKRWTRDARDGVIIDHIGNEVVDDPKLFSTHRYKRLCSTMIRLAADVSTSEYLHKLVEKGLYDLCKLVMEARLREIENADDEGDAPTVTKEYESQPKGFKIKPGLQNRRRLKSCVELMSKKKSKATIVKPTPRQRSKV