AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032171

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032171.t1 MTR_2g098880 95.635 252 11 0 1 252 1 252 0.0 500
MS.gene032171.t1 MTR_2g098880 95.635 252 11 0 1 252 13 264 0.0 499
MS.gene032171.t1 MTR_3g006370 36.269 193 103 5 55 243 44 220 4.02e-35 125
MS.gene032171.t1 MTR_2g098920 37.634 186 101 5 60 243 79 251 5.85e-34 123
MS.gene032171.t1 MTR_4g067290 37.222 180 103 4 60 239 55 224 1.35e-31 116
MS.gene032171.t1 MTR_4g067290 37.222 180 103 4 60 239 75 244 1.44e-31 117
MS.gene032171.t1 MTR_4g067290 37.222 180 103 4 60 239 115 284 5.24e-31 116
MS.gene032171.t1 MTR_4g067290 37.158 183 97 6 60 239 55 222 1.05e-30 114
MS.gene032171.t1 MTR_4g067290 37.158 183 97 6 60 239 73 240 1.39e-30 114
MS.gene032171.t1 MTR_4g067290 37.158 183 97 6 60 239 75 242 1.39e-30 114
MS.gene032171.t1 MTR_7g028855 32.941 170 98 6 11 170 65 228 4.32e-12 65.9
MS.gene032171.t1 MTR_1g057920 33.884 121 71 4 32 151 11 123 1.78e-11 60.5
MS.gene032171.t1 MTR_1g070355 33.333 102 66 2 65 165 78 178 3.18e-11 63.2
MS.gene032171.t1 MTR_2g070590 34.286 105 66 2 61 164 17 119 6.88e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032171.t1 AT2G43280 43.077 195 89 6 54 243 15 192 7.73e-42 142
MS.gene032171.t1 AT3G07500 39.894 188 106 3 56 243 28 208 1.68e-39 136
MS.gene032171.t1 AT2G43280 44.509 173 74 6 76 243 2 157 1.63e-37 130
MS.gene032171.t1 AT3G59470 35.586 222 124 7 31 244 32 242 3.65e-35 126
MS.gene032171.t1 AT3G59470 35.586 222 124 7 31 244 32 242 3.65e-35 126
MS.gene032171.t1 AT3G59470 35.586 222 124 7 31 244 44 254 3.87e-35 126
MS.gene032171.t1 AT4G12850 34.783 184 104 4 60 243 10 177 4.71e-33 119
MS.gene032171.t1 AT4G38180 44.262 122 67 1 50 170 62 183 1.15e-23 100
MS.gene032171.t1 AT4G12850 39.048 105 59 1 60 164 10 109 4.98e-22 89.0
MS.gene032171.t1 AT4G12850 39.048 105 59 1 60 164 10 109 5.12e-22 89.0
MS.gene032171.t1 AT5G18960 42.453 106 46 1 59 164 41 131 5.04e-21 92.4
MS.gene032171.t1 AT5G18960 35.238 105 53 1 60 164 210 299 2.61e-15 75.5
MS.gene032171.t1 AT5G18960 42.453 106 46 1 59 164 41 131 5.04e-21 92.4
MS.gene032171.t1 AT5G18960 35.238 105 53 1 60 164 210 299 2.61e-15 75.5
MS.gene032171.t1 AT3G06250 41.509 106 47 1 59 164 26 116 2.83e-20 90.1
MS.gene032171.t1 AT3G06250 36.066 122 59 2 43 164 176 278 1.12e-16 79.7
MS.gene032171.t1 AT3G06250 41.509 106 47 1 59 164 26 116 2.83e-20 90.1
MS.gene032171.t1 AT3G06250 36.066 122 59 2 43 164 176 278 1.12e-16 79.7
MS.gene032171.t1 AT3G06250 41.509 106 47 1 59 164 26 116 2.83e-20 90.1
MS.gene032171.t1 AT3G06250 36.066 122 59 2 43 164 176 278 1.12e-16 79.7
MS.gene032171.t1 AT2G27110 32.044 181 85 5 41 200 28 191 5.34e-15 74.7
MS.gene032171.t1 AT2G27110 32.044 181 85 5 41 200 28 191 5.34e-15 74.7
MS.gene032171.t1 AT3G22170 31.707 123 73 1 59 170 68 190 1.43e-14 73.6
MS.gene032171.t1 AT3G22170 31.707 123 73 1 59 170 68 190 1.43e-14 73.6
MS.gene032171.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.56e-13 70.5
MS.gene032171.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.58e-13 70.5
MS.gene032171.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.58e-13 70.5
MS.gene032171.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.63e-13 70.5
MS.gene032171.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.63e-13 70.5
MS.gene032171.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.63e-13 70.5
MS.gene032171.t1 AT4G19990 31.579 114 67 1 64 166 24 137 7.96e-13 68.2
MS.gene032171.t1 AT4G19990 31.579 114 67 1 64 166 24 137 7.96e-13 68.2
MS.gene032171.t1 AT4G19990 31.579 114 67 1 64 166 38 151 8.00e-13 68.2
MS.gene032171.t1 AT4G19990 31.579 114 67 1 64 166 24 137 8.03e-13 68.2
MS.gene032171.t1 AT4G19990 31.579 114 67 1 64 166 24 137 8.72e-13 68.2
MS.gene032171.t1 AT4G19990 31.579 114 67 1 64 166 24 137 8.72e-13 68.2
MS.gene032171.t1 AT2G32250 34.000 100 61 1 65 164 1 95 1.25e-12 67.8
MS.gene032171.t1 AT2G32250 34.000 100 61 1 65 164 1 95 1.27e-12 67.8
MS.gene032171.t1 AT1G76320 30.159 126 82 2 65 190 1 120 2.49e-12 66.6
MS.gene032171.t1 AT1G76320 30.159 126 82 2 65 190 1 120 2.49e-12 66.6
MS.gene032171.t1 AT1G76320 32.673 101 63 1 65 165 1 96 2.51e-12 66.6
MS.gene032171.t1 AT1G76320 30.159 126 82 2 65 190 1 120 2.56e-12 66.6
MS.gene032171.t1 AT1G80010 33.654 104 63 1 61 164 95 192 4.55e-12 65.9
MS.gene032171.t1 AT4G15090 31.776 107 71 1 59 165 49 153 4.84e-12 65.9
MS.gene032171.t1 AT4G15090 31.776 107 71 1 59 165 49 153 4.84e-12 65.9
MS.gene032171.t1 AT4G15090 31.776 107 71 1 59 165 49 153 4.84e-12 65.9
MS.gene032171.t1 AT4G15090 31.776 107 71 1 59 165 49 153 4.84e-12 65.9
MS.gene032171.t1 AT4G15090 31.776 107 71 1 59 165 49 153 4.84e-12 65.9
MS.gene032171.t1 AT1G52520 32.692 104 64 1 61 164 85 182 9.14e-12 65.1

Find 62 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGATAAGGTTGGCTTTAC+GGG 0.249268 2.3:-4576402 MS.gene032171:CDS
TGTATGTTCCTTCACAAATT+TGG 0.258446 2.3:+4576358 None:intergenic
CCATGATAAGGTTGGCTTTA+CGG 0.274945 2.3:-4576403 MS.gene032171:CDS
TCTCGGGTGGCTGGTGGAGG+AGG 0.303392 2.3:+4576437 None:intergenic
CCTCAATCTCTCTGATATTT+CGG 0.319830 2.3:+4575320 None:intergenic
AAATACAATTCTGAACGATT+TGG 0.340360 2.3:-4575386 MS.gene032171:CDS
TTACTCGCGAACGGCGGTTA+TGG 0.347299 2.3:+4576546 None:intergenic
CGAGTAAATAACAAGCTAAT+TGG 0.351629 2.3:-4576530 MS.gene032171:CDS
TCATGGCTTGACAACCTTCT+CGG 0.378795 2.3:+4576420 None:intergenic
CCTTCTCGGGTGGCTGGTGG+AGG 0.389419 2.3:+4576434 None:intergenic
GAAGTTCCATGGCGAGGATC+CGG 0.395395 2.3:-4576314 MS.gene032171:CDS
CTCACTAACCAAGTGCTTCC+CGG 0.404686 2.3:+4576295 None:intergenic
CATGGCTTGACAACCTTCTC+GGG 0.412731 2.3:+4576421 None:intergenic
TGACAACCTTCTCGGGTGGC+TGG 0.424878 2.3:+4576428 None:intergenic
TCCTCGCCATGGAACTTCAC+TGG 0.443266 2.3:+4576319 None:intergenic
AGGATGATGGATCACCAATC+TGG 0.443741 2.3:-4576821 MS.gene032171:CDS
GACACATTAGAACCATTCAT+AGG 0.454194 2.3:-4576650 MS.gene032171:CDS
TGTCACAGGACGAGAAAGAT+AGG 0.454669 2.3:-4575489 MS.gene032171:intron
AATCAAATCCCTGGCCAGAT+TGG 0.455766 2.3:+4576807 None:intergenic
CGAACAACCTGTAGCAGATT+CGG 0.459103 2.3:-4576672 MS.gene032171:CDS
CCGAAATATCAGAGAGATTG+AGG 0.462386 2.3:-4575320 MS.gene032171:CDS
CTCGGGTGGCTGGTGGAGGA+GGG 0.466145 2.3:+4576438 None:intergenic
GATGGATCACCAATCTGGCC+AGG 0.492007 2.3:-4576816 MS.gene032171:CDS
CAACCTTCTCGGGTGGCTGG+TGG 0.497367 2.3:+4576431 None:intergenic
AGGTTGGCTTTACGGGATGA+AGG 0.498475 2.3:-4576395 MS.gene032171:CDS
AAGTTCCATGGCGAGGATCC+GGG 0.502060 2.3:-4576313 MS.gene032171:CDS
AAGGTTGTCAAGCCATGATA+AGG 0.504631 2.3:-4576415 MS.gene032171:CDS
ACGAAAGGCAAAAGTACAAA+AGG 0.509895 2.3:-4575435 MS.gene032171:CDS
CTTTACGGGATGAAGGGAAG+TGG 0.510166 2.3:-4576388 MS.gene032171:CDS
CCTCGCCATGGAACTTCACT+GGG 0.518653 2.3:+4576320 None:intergenic
AATGTGTCCGAATCTGCTAC+AGG 0.521451 2.3:+4576665 None:intergenic
AACAATTTAGAATTATGTAC+AGG 0.522470 2.3:-4576713 MS.gene032171:CDS
CGCTCGAGTTGCACAACGAA+AGG 0.523644 2.3:-4575450 MS.gene032171:CDS
CCGTAAAGCCAACCTTATCA+TGG 0.524530 2.3:+4576403 None:intergenic
GGCCAGGGATTTGATTCAGA+TGG 0.527040 2.3:-4576800 MS.gene032171:CDS
AATGGCCATGGTAGGAGGAT+AGG 0.534923 2.3:-4576587 MS.gene032171:CDS
GCGAGGATCCGGGAAGCACT+TGG 0.541373 2.3:-4576303 MS.gene032171:CDS
CCTCCACCAGCCACCCGAGA+AGG 0.543028 2.3:-4576434 MS.gene032171:CDS
TGCTTCCCGGATCCTCGCCA+TGG 0.557183 2.3:+4576308 None:intergenic
TTTACGGGATGAAGGGAAGT+GGG 0.561477 2.3:-4576387 MS.gene032171:CDS
AACCATCTGAATCAAATCCC+TGG 0.570761 2.3:+4576798 None:intergenic
GGTTGGCTTTACGGGATGAA+GGG 0.571058 2.3:-4576394 MS.gene032171:CDS
TAGGCATGCAGTTCAACTCA+AGG 0.572467 2.3:-4576631 MS.gene032171:CDS
TCTACAATGGCCATGGTAGG+AGG 0.572483 2.3:-4576592 MS.gene032171:CDS
GCTTGTTATTTACTCGCGAA+CGG 0.583871 2.3:+4576537 None:intergenic
GGAGGATAGGATTTACAGTA+CGG 0.583900 2.3:-4576574 MS.gene032171:CDS
AGAGAATTCTACAATGGCCA+TGG 0.595180 2.3:-4576599 MS.gene032171:CDS
TTGTCAAGCCATGATAAGGT+TGG 0.599156 2.3:-4576411 MS.gene032171:CDS
CCCAGTGAAGTTCCATGGCG+AGG 0.602502 2.3:-4576320 MS.gene032171:CDS
TAAATCCTATCCTCCTACCA+TGG 0.603101 2.3:+4576582 None:intergenic
TTGAACTGCATGCCTATGAA+TGG 0.604946 2.3:+4576638 None:intergenic
TGAGTCCCAGTGAAGTTCCA+TGG 0.607822 2.3:-4576325 MS.gene032171:CDS
ATGGATCACCAATCTGGCCA+GGG 0.613451 2.3:-4576815 MS.gene032171:CDS
AATTCTACAATGGCCATGGT+AGG 0.613869 2.3:-4576595 MS.gene032171:CDS
GGCTTGACAACCTTCTCGGG+TGG 0.617601 2.3:+4576424 None:intergenic
GGTTGTCACCAAATTTGTGA+AGG 0.624088 2.3:-4576366 MS.gene032171:CDS
CGGGAAGCACTTGGTTAGTG+AGG 0.627824 2.3:-4576294 MS.gene032171:intron
AGGCATGCAGTTCAACTCAA+GGG 0.634792 2.3:-4576630 MS.gene032171:CDS
TGTTATTTACTCGCGAACGG+CGG 0.635708 2.3:+4576540 None:intergenic
GCAACTCGAGCGATAGCTCA+CGG 0.642945 2.3:+4575461 None:intergenic
GAAGCAAGAGAATTCTACAA+TGG 0.657489 2.3:-4576605 MS.gene032171:CDS
CATGCAGTTCAACTCAAGGG+AGG 0.700261 2.3:-4576627 MS.gene032171:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GACTATATTATGTATATTCT+TGG + chr2.3:4576376-4576395 None:intergenic 20.0%
!! TATCAAAATACAGATTCTTA+TGG - chr2.3:4576113-4576132 MS.gene032171:intron 20.0%
!!! AACAATTTAGAATTATGTAC+AGG - chr2.3:4575396-4575415 MS.gene032171:CDS 20.0%
!!! TTTTAGAATATCTTATCTCT+GGG - chr2.3:4576139-4576158 MS.gene032171:intron 20.0%
! AAATACAATTCTGAACGATT+TGG - chr2.3:4576723-4576742 MS.gene032171:CDS 25.0%
! GAAGAAAAATCAGATTTAGA+TGG - chr2.3:4576811-4576830 MS.gene032171:CDS 25.0%
! TAAACTATAAAGTGCACTAT+TGG + chr2.3:4575850-4575869 None:intergenic 25.0%
!! AGGATTGTATTAACTCTTTT+AGG - chr2.3:4576452-4576471 MS.gene032171:CDS 25.0%
!! GGATTGTATTAACTCTTTTA+GGG - chr2.3:4576453-4576472 MS.gene032171:CDS 25.0%
!!! CCTGTTTGAGATTTTTTTTA+GGG + chr2.3:4576435-4576454 None:intergenic 25.0%
!!! CTGTTTGAGATTTTTTTTAG+GGG + chr2.3:4576434-4576453 None:intergenic 25.0%
!!! CTTTTAGAATATCTTATCTC+TGG - chr2.3:4576138-4576157 MS.gene032171:intron 25.0%
!!! TCCTGTTTGAGATTTTTTTT+AGG + chr2.3:4576436-4576455 None:intergenic 25.0%
!!! TGTTTGAGATTTTTTTTAGG+GGG + chr2.3:4576433-4576452 None:intergenic 25.0%
!!! TTCTAGAATACCTTATCTAT+GGG - chr2.3:4576042-4576061 MS.gene032171:intron 25.0%
AAAAAGGAACAAACAGAACA+TGG + chr2.3:4576572-4576591 None:intergenic 30.0%
AAAAATCAGATTTAGATGGC+AGG - chr2.3:4576815-4576834 MS.gene032171:CDS 30.0%
AAATCAACAAGACAAGTTTG+AGG + chr2.3:4576599-4576618 None:intergenic 30.0%
ATTAAAAGTCCCATCTCAAT+CGG - chr2.3:4576477-4576496 MS.gene032171:CDS 30.0%
CCCTAAAAAAAATCTCAAAC+AGG - chr2.3:4576432-4576451 MS.gene032171:CDS 30.0%
CGAGTAAATAACAAGCTAAT+TGG - chr2.3:4575579-4575598 MS.gene032171:intron 30.0%
GAAAATTGTGAGTGAAAGTT+TGG + chr2.3:4576167-4576186 None:intergenic 30.0%
GTATAGTTCAGTTCTTTGTT+AGG - chr2.3:4576537-4576556 MS.gene032171:CDS 30.0%
TAGCAAAATACAGATTCTCA+TGG - chr2.3:4576016-4576035 MS.gene032171:intron 30.0%
TATGTATACCTGATCAAACT+TGG - chr2.3:4575881-4575900 MS.gene032171:intron 30.0%
TGTATGTTCCTTCACAAATT+TGG + chr2.3:4575754-4575773 None:intergenic 30.0%
! ACAAGTTTGAGGTAGAAAAA+AGG + chr2.3:4576588-4576607 None:intergenic 30.0%
! ATTCTTGGTGTAATATCGAT+AGG + chr2.3:4576361-4576380 None:intergenic 30.0%
! TAGCAAGAGAATTTAAGACA+TGG + chr2.3:4576313-4576332 None:intergenic 30.0%
!! CTTCTAGAATACCTTATCTA+TGG - chr2.3:4576041-4576060 MS.gene032171:intron 30.0%
CCTCAATCTCTCTGATATTT+CGG + chr2.3:4576792-4576811 None:intergenic 35.0%
GAAAAATGTGAGTGAAGGTT+TGG + chr2.3:4576070-4576089 None:intergenic 35.0%
GAAGCAAGAGAATTCTACAA+TGG - chr2.3:4575504-4575523 MS.gene032171:intron 35.0%
GACACATTAGAACCATTCAT+AGG - chr2.3:4575459-4575478 MS.gene032171:CDS 35.0%
TTACATGCAGATGTTGGATT+AGG - chr2.3:4576500-4576519 MS.gene032171:CDS 35.0%
TTCCACCTAAGATAGAATGA+GGG - chr2.3:4576335-4576354 MS.gene032171:CDS 35.0%
TTTCCACCTAAGATAGAATG+AGG - chr2.3:4576334-4576353 MS.gene032171:CDS 35.0%
! ACGAAAGGCAAAAGTACAAA+AGG - chr2.3:4576674-4576693 MS.gene032171:CDS 35.0%
! GATTTTGTCTGCTCAAAAGA+AGG - chr2.3:4575606-4575625 MS.gene032171:intron 35.0%
!! GTCAGTCTCACTTTTAAATC+TGG + chr2.3:4575907-4575926 None:intergenic 35.0%
!!! TTGAGATTTTTTTTAGGGGG+TGG + chr2.3:4576430-4576449 None:intergenic 35.0%
AAATCTGGCCAAGTTTGATC+AGG + chr2.3:4575892-4575911 None:intergenic 40.0%
AACCATCTGAATCAAATCCC+TGG + chr2.3:4575314-4575333 None:intergenic 40.0%
AAGGTTGTCAAGCCATGATA+AGG - chr2.3:4575694-4575713 MS.gene032171:intron 40.0%
AATCGGTTACATGCAGATGT+TGG - chr2.3:4576494-4576513 MS.gene032171:CDS 40.0%
AATTCTACAATGGCCATGGT+AGG - chr2.3:4575514-4575533 MS.gene032171:intron 40.0%
AGAGAATTCTACAATGGCCA+TGG - chr2.3:4575510-4575529 MS.gene032171:intron 40.0%
AGATTGACAACAGTATCAGC+AGG - chr2.3:4575962-4575981 MS.gene032171:intron 40.0%
ATAGGCCCTCATTCTATCTT+AGG + chr2.3:4576343-4576362 None:intergenic 40.0%
ATCAGTGAGGGACTTAGAAA+AGG + chr2.3:4576210-4576229 None:intergenic 40.0%
CCGAAATATCAGAGAGATTG+AGG - chr2.3:4576789-4576808 MS.gene032171:CDS 40.0%
GGAGGATAGGATTTACAGTA+CGG - chr2.3:4575535-4575554 MS.gene032171:intron 40.0%
GGTTGTCACCAAATTTGTGA+AGG - chr2.3:4575743-4575762 MS.gene032171:intron 40.0%
GTCAATCTAATATCAGCAGC+AGG + chr2.3:4575950-4575969 None:intergenic 40.0%
TAAATCCTATCCTCCTACCA+TGG + chr2.3:4575530-4575549 None:intergenic 40.0%
TCAGTGAGGGACTTAGAAAA+GGG + chr2.3:4576209-4576228 None:intergenic 40.0%
TGATGCAAATGGAAGGGAAA+GGG + chr2.3:4576243-4576262 None:intergenic 40.0%
TGCATGTAACCGATTGAGAT+GGG + chr2.3:4576489-4576508 None:intergenic 40.0%
TTGAACTGCATGCCTATGAA+TGG + chr2.3:4575474-4575493 None:intergenic 40.0%
TTGTCAAGCCATGATAAGGT+TGG - chr2.3:4575698-4575717 MS.gene032171:intron 40.0%
! AGGTTTGGAACCCATAGATA+AGG + chr2.3:4576055-4576074 None:intergenic 40.0%
! GGATTTTGTTGGATCAGTGA+GGG + chr2.3:4576222-4576241 None:intergenic 40.0%
! TTCGAGTTCATCATGTAGAG+CGG + chr2.3:4576093-4576112 None:intergenic 40.0%
!! CATGATAAGGTTGGCTTTAC+GGG - chr2.3:4575707-4575726 MS.gene032171:intron 40.0%
!! CCATGATAAGGTTGGCTTTA+CGG - chr2.3:4575706-4575725 MS.gene032171:intron 40.0%
!! GCTTGTTATTTACTCGCGAA+CGG + chr2.3:4575575-4575594 None:intergenic 40.0%
!! GTCTTGTTGATTTGTGTCAC+AGG - chr2.3:4576606-4576625 MS.gene032171:CDS 40.0%
!!! AGATTTTTTTTAGGGGGTGG+AGG + chr2.3:4576427-4576446 None:intergenic 40.0%
AACGACGTGATGCAAATGGA+AGG + chr2.3:4576250-4576269 None:intergenic 45.0%
AATATCAGCAGCAGGAGAAG+AGG + chr2.3:4575942-4575961 None:intergenic 45.0%
AATCAAATCCCTGGCCAGAT+TGG + chr2.3:4575305-4575324 None:intergenic 45.0%
AATGTGTCCGAATCTGCTAC+AGG + chr2.3:4575447-4575466 None:intergenic 45.0%
ACGACGTGATGCAAATGGAA+GGG + chr2.3:4576249-4576268 None:intergenic 45.0%
AGCGGGAAAAATGTGAGTGA+AGG + chr2.3:4576075-4576094 None:intergenic 45.0%
AGGCATGCAGTTCAACTCAA+GGG - chr2.3:4575479-4575498 MS.gene032171:CDS 45.0%
ATGGCAGCATGAGACACATT+GGG + chr2.3:4576294-4576313 None:intergenic 45.0%
CCGTAAAGCCAACCTTATCA+TGG + chr2.3:4575709-4575728 None:intergenic 45.0%
CGAACAACCTGTAGCAGATT+CGG - chr2.3:4575437-4575456 MS.gene032171:CDS 45.0%
CTGCATGTAACCGATTGAGA+TGG + chr2.3:4576490-4576509 None:intergenic 45.0%
GTGATGCAAATGGAAGGGAA+AGG + chr2.3:4576244-4576263 None:intergenic 45.0%
TAGGCATGCAGTTCAACTCA+AGG - chr2.3:4575478-4575497 MS.gene032171:CDS 45.0%
TCATGGCTTGACAACCTTCT+CGG + chr2.3:4575692-4575711 None:intergenic 45.0%
TCCATTTGCATCACGTCGTT+AGG - chr2.3:4576250-4576269 MS.gene032171:intron 45.0%
TCCTAACGACGTGATGCAAA+TGG + chr2.3:4576254-4576273 None:intergenic 45.0%
TCGAGTTCATCATGTAGAGC+GGG + chr2.3:4576092-4576111 None:intergenic 45.0%
TGTCACAGGACGAGAAAGAT+AGG - chr2.3:4576620-4576639 MS.gene032171:CDS 45.0%
TTTACGGGATGAAGGGAAGT+GGG - chr2.3:4575722-4575741 MS.gene032171:intron 45.0%
! AAAAGGGTGTGTGGAAAGTG+AGG + chr2.3:4576193-4576212 None:intergenic 45.0%
! AGGACTAGAGGTAGCTTTTC+AGG + chr2.3:4576407-4576426 None:intergenic 45.0%
! GGAAGGGAAAGGGATTTTGT+TGG + chr2.3:4576233-4576252 None:intergenic 45.0%
! GGGATTTTGTTGGATCAGTG+AGG + chr2.3:4576223-4576242 None:intergenic 45.0%
! TGTTATTTACTCGCGAACGG+CGG + chr2.3:4575572-4575591 None:intergenic 45.0%
AATGGCCATGGTAGGAGGAT+AGG - chr2.3:4575522-4575541 MS.gene032171:intron 50.0%
ATGGATCACCAATCTGGCCA+GGG - chr2.3:4575294-4575313 MS.gene032171:CDS 50.0%
CATGCAGTTCAACTCAAGGG+AGG - chr2.3:4575482-4575501 MS.gene032171:CDS 50.0%
CATGGCAGCATGAGACACAT+TGG + chr2.3:4576295-4576314 None:intergenic 50.0%
CATGGCTTGACAACCTTCTC+GGG + chr2.3:4575691-4575710 None:intergenic 50.0%
CTCACTAACCAAGTGCTTCC+CGG + chr2.3:4575817-4575836 None:intergenic 50.0%
CTTTACGGGATGAAGGGAAG+TGG - chr2.3:4575721-4575740 MS.gene032171:intron 50.0%
GGCCAGGGATTTGATTCAGA+TGG - chr2.3:4575309-4575328 MS.gene032171:CDS 50.0%
GGCCCTCATTCTATCTTAGG+TGG + chr2.3:4576340-4576359 None:intergenic 50.0%
TCTACAATGGCCATGGTAGG+AGG - chr2.3:4575517-4575536 MS.gene032171:intron 50.0%
TGAGTCCCAGTGAAGTTCCA+TGG - chr2.3:4575784-4575803 MS.gene032171:intron 50.0%
! GGGACTTAGAAAAGGGTGTG+TGG + chr2.3:4576202-4576221 None:intergenic 50.0%
!! AGGTTGGCTTTACGGGATGA+AGG - chr2.3:4575714-4575733 MS.gene032171:intron 50.0%
!! GGTTGGCTTTACGGGATGAA+GGG - chr2.3:4575715-4575734 MS.gene032171:intron 50.0%
AAGTTCCATGGCGAGGATCC+GGG - chr2.3:4575796-4575815 MS.gene032171:intron 55.0%
CCTCGCCATGGAACTTCACT+GGG + chr2.3:4575792-4575811 None:intergenic 55.0%
GAAGTTCCATGGCGAGGATC+CGG - chr2.3:4575795-4575814 MS.gene032171:intron 55.0%
GATGGATCACCAATCTGGCC+AGG - chr2.3:4575293-4575312 MS.gene032171:CDS 55.0%
GCAACTCGAGCGATAGCTCA+CGG + chr2.3:4576651-4576670 None:intergenic 55.0%
TCCTCGCCATGGAACTTCAC+TGG + chr2.3:4575793-4575812 None:intergenic 55.0%
TTACTCGCGAACGGCGGTTA+TGG + chr2.3:4575566-4575585 None:intergenic 55.0%
TTTAGGGGGTGGAGGACTAG+AGG + chr2.3:4576419-4576438 None:intergenic 55.0%
! CGCTCGAGTTGCACAACGAA+AGG - chr2.3:4576659-4576678 MS.gene032171:CDS 55.0%
!! CGGGAAGCACTTGGTTAGTG+AGG - chr2.3:4575815-4575834 MS.gene032171:intron 55.0%
CCCAGTGAAGTTCCATGGCG+AGG - chr2.3:4575789-4575808 MS.gene032171:intron 60.0%
GGCTTGACAACCTTCTCGGG+TGG + chr2.3:4575688-4575707 None:intergenic 60.0%
GGGTGTGTGGAAAGTGAGGC+TGG + chr2.3:4576189-4576208 None:intergenic 60.0%
TGACAACCTTCTCGGGTGGC+TGG + chr2.3:4575684-4575703 None:intergenic 60.0%
CAACCTTCTCGGGTGGCTGG+TGG + chr2.3:4575681-4575700 None:intergenic 65.0%
TGCTTCCCGGATCCTCGCCA+TGG + chr2.3:4575804-4575823 None:intergenic 65.0%
!! GCGAGGATCCGGGAAGCACT+TGG - chr2.3:4575806-4575825 MS.gene032171:intron 65.0%
CCTCCACCAGCCACCCGAGA+AGG - chr2.3:4575675-4575694 MS.gene032171:intron 70.0%
CCTTCTCGGGTGGCTGGTGG+AGG + chr2.3:4575678-4575697 None:intergenic 70.0%
CTCGGGTGGCTGGTGGAGGA+GGG + chr2.3:4575674-4575693 None:intergenic 70.0%
TCTCGGGTGGCTGGTGGAGG+AGG + chr2.3:4575675-4575694 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 4575291 4576840 4575291 ID=MS.gene032171
chr2.3 mRNA 4575291 4576840 4575291 ID=MS.gene032171.t1;Parent=MS.gene032171
chr2.3 exon 4576295 4576840 4576295 ID=MS.gene032171.t1.exon1;Parent=MS.gene032171.t1
chr2.3 CDS 4576295 4576840 4576295 ID=cds.MS.gene032171.t1;Parent=MS.gene032171.t1
chr2.3 exon 4575291 4575503 4575291 ID=MS.gene032171.t1.exon2;Parent=MS.gene032171.t1
chr2.3 CDS 4575291 4575503 4575291 ID=cds.MS.gene032171.t1;Parent=MS.gene032171.t1
Gene Sequence

>MS.gene032171

ATGATGGATCACCAATCTGGCCAGGGATTTGATTCAGATGGTTGTGACTTTGATGTGCTAGCAGAAATCAGTGAGAAACATGAGAAAGTAGAAGATGAGTTTACAAACAATTTAGAATTATGTACAGGCGAAGATGAAAAGTTTGTCGAACAACCTGTAGCAGATTCGGACACATTAGAACCATTCATAGGCATGCAGTTCAACTCAAGGGAGGAAGCAAGAGAATTCTACAATGGCCATGGTAGGAGGATAGGATTTACAGTACGGATACACCATAACCGCCGTTCGCGAGTAAATAACAAGCTAATTGGTCAAGATTTTGTCTGCTCAAAAGAAGGTTTTCGTGCAAAAAAGTATGTGCACAGAAAAGATAGAGTGCTCCCTCCTCCACCAGCCACCCGAGAAGGTTGTCAAGCCATGATAAGGTTGGCTTTACGGGATGAAGGGAAGTGGGTTGTCACCAAATTTGTGAAGGAACATACACATAAACTGATGAGTCCCAGTGAAGTTCCATGGCGAGGATCCGGGAAGCACTTGGTTAGTGAGGTACGTTTCTCCAATAGTGCACTTTATAGTTTAAACTAACAGTTTATGTATACCTGATCAAACTTGGCCAGATTTAAAAGTGAGACTGACTAATGCATTATACCTCTTCTCCTGCTGCTGATATTAGATTGACAACAGTATCAGCAGGATTAAACTACACTGAACATGATGAACTCGAATAGCAAAATACAGATTCTCATGGAGCTTCTAGAATACCTTATCTATGGGTTCCAAACCTTCACTCACATTTTTCCCGCTCTACATGATGAACTCGAATATCAAAATACAGATTCTTATGGAGCTTTTAGAATATCTTATCTCTGGGTTCCAAACTTTCACTCACAATTTTCCAGCCTCACTTTCCACACACCCTTTTCTAAGTCCCTCACTGATCCAACAAAATCCCTTTCCCTTCCATTTGCATCACGTCGTTAGGAAGTTAGTTATCTGAAGTCCCAATGTGTCTCATGCTGCCATGTCTTAAATTCTCTTGCTATTTTCCACCTAAGATAGAATGAGGGCCTATCGATATTACACCAAGAATATACATAATATAGTCAATTATAGCCTGAAAAGCTACCTCTAGTCCTCCACCCCCTAAAAAAAATCTCAAACAGGATTGTATTAACTCTTTTAGGGCATTAAAAGTCCCATCTCAATCGGTTACATGCAGATGTTGGATTAGGATATTTTGATTTGTGTATAGTTCAGTTCTTTGTTAGGATTGAATGTCCATGTTCTGTTTGTTCCTTTTTTCTACCTCAAACTTGTCTTGTTGATTTGTGTCACAGGACGAGAAAGATAGGAGAATCCGTGAGCTATCGCTCGAGTTGCACAACGAAAGGCAAAAGTACAAAAGGCGTTGTGCAGCATATGAAGAACAGTTAAATACAATTCTGAACGATTTGGAAAAGCACACAGAACATATCTCTAAAAAAGTTGCTGATGTAGTCCGAAATATCAGAGAGATTGAGGAAGAAAAATCAGATTTAGATGGCAGGTAA
Protein sequence

>MS.gene032171.t1

MMDHQSGQGFDSDGCDFDVLAEISEKHEKVEDEFTNNLELCTGEDEKFVEQPVADSDTLEPFIGMQFNSREEAREFYNGHGRRIGFTVRIHHNRRSRVNNKLIGQDFVCSKEGFRAKKYVHRKDRVLPPPPATREGCQAMIRLALRDEGKWVVTKFVKEHTHKLMSPSEVPWRGSGKHLVSEDEKDRRIRELSLELHNERQKYKRRCAAYEEQLNTILNDLEKHTEHISKKVADVVRNIREIEEEKSDLDGR