AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032176

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032176.t1 MTR_2g098920 95.367 259 12 0 28 286 6 264 0.0 509
MS.gene032176.t1 MTR_4g067290 65.289 242 82 2 28 269 3 242 2.97e-110 319
MS.gene032176.t1 MTR_4g067290 64.876 242 83 2 28 269 1 240 5.83e-110 318
MS.gene032176.t1 MTR_4g067290 65.164 244 81 3 28 269 3 244 4.63e-109 317
MS.gene032176.t1 MTR_4g067290 65.041 246 82 3 26 269 41 284 5.54e-109 318
MS.gene032176.t1 MTR_4g067290 68.349 218 68 1 52 269 6 222 3.76e-106 308
MS.gene032176.t1 MTR_4g067290 68.182 220 67 2 52 269 6 224 9.07e-105 305
MS.gene032176.t1 MTR_3g006370 37.559 213 120 4 73 277 17 224 7.16e-40 139
MS.gene032176.t1 MTR_2g098880 36.757 185 104 4 101 273 60 243 4.14e-34 124
MS.gene032176.t1 MTR_2g098880 36.757 185 104 4 101 273 72 255 6.26e-34 124
MS.gene032176.t1 MTR_5g015480 35.971 139 67 5 102 224 56 188 1.42e-17 80.9
MS.gene032176.t1 MTR_1g057920 45.679 81 43 1 102 181 34 114 5.38e-16 73.2
MS.gene032176.t1 MTR_0402s0040 38.318 107 60 3 102 204 26 130 1.22e-13 71.2
MS.gene032176.t1 MTR_2g070590 38.462 104 60 3 102 202 17 119 6.85e-13 64.7
MS.gene032176.t1 MTR_6g017200 40.741 81 47 1 102 181 58 138 6.61e-12 62.8
MS.gene032176.t1 MTR_6g051425 31.579 114 70 3 99 204 11 124 5.18e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032176.t1 AT3G59470 67.391 184 60 0 100 283 80 263 5.31e-94 278
MS.gene032176.t1 AT3G59470 67.391 184 60 0 100 283 68 251 5.88e-94 278
MS.gene032176.t1 AT3G59470 67.391 184 60 0 100 283 68 251 5.88e-94 278
MS.gene032176.t1 AT3G07500 42.487 193 100 4 93 274 17 209 8.45e-46 154
MS.gene032176.t1 AT2G43280 36.792 212 113 4 70 277 2 196 4.20e-36 128
MS.gene032176.t1 AT4G12850 34.973 183 106 3 99 277 8 181 3.70e-33 120
MS.gene032176.t1 AT2G43280 39.394 165 91 3 117 277 2 161 1.37e-31 115
MS.gene032176.t1 AT4G38180 56.481 108 43 2 101 204 72 179 1.92e-24 103
MS.gene032176.t1 AT4G12850 44.231 104 56 1 99 202 8 109 1.15e-23 94.4
MS.gene032176.t1 AT4G12850 44.231 104 56 1 99 202 8 109 1.45e-23 94.0
MS.gene032176.t1 AT3G06250 44.118 102 45 1 101 202 27 116 5.52e-21 93.2
MS.gene032176.t1 AT3G06250 41.509 106 50 1 101 206 189 282 1.13e-20 92.4
MS.gene032176.t1 AT3G06250 44.118 102 45 1 101 202 27 116 5.52e-21 93.2
MS.gene032176.t1 AT3G06250 41.509 106 50 1 101 206 189 282 1.13e-20 92.4
MS.gene032176.t1 AT3G06250 44.118 102 45 1 101 202 27 116 5.52e-21 93.2
MS.gene032176.t1 AT3G06250 41.509 106 50 1 101 206 189 282 1.13e-20 92.4
MS.gene032176.t1 AT5G18960 43.137 102 46 1 101 202 42 131 1.46e-20 92.0
MS.gene032176.t1 AT5G18960 40.367 109 53 1 98 206 207 303 2.27e-20 91.3
MS.gene032176.t1 AT5G18960 43.137 102 46 1 101 202 42 131 1.46e-20 92.0
MS.gene032176.t1 AT5G18960 40.367 109 53 1 98 206 207 303 2.27e-20 91.3
MS.gene032176.t1 AT1G52520 36.792 106 64 1 97 202 80 182 4.08e-16 79.0
MS.gene032176.t1 AT1G80010 36.792 106 64 1 102 207 95 197 8.33e-15 74.7
MS.gene032176.t1 AT4G19990 30.000 150 75 3 99 234 35 168 5.62e-14 72.4
MS.gene032176.t1 AT4G19990 30.000 150 75 3 99 234 21 154 5.85e-14 72.4
MS.gene032176.t1 AT4G19990 30.000 150 75 3 99 234 21 154 5.85e-14 72.4
MS.gene032176.t1 AT4G19990 30.000 150 75 3 99 234 21 154 6.18e-14 72.4
MS.gene032176.t1 AT4G19990 30.000 150 75 3 99 234 21 154 6.54e-14 72.4
MS.gene032176.t1 AT4G19990 30.000 150 75 3 99 234 21 154 6.54e-14 72.4
MS.gene032176.t1 AT2G32250 31.915 141 88 3 68 204 2 138 1.28e-13 71.2
MS.gene032176.t1 AT2G32250 31.915 141 88 3 68 204 2 138 1.31e-13 71.2
MS.gene032176.t1 AT2G32250 31.915 141 88 3 68 204 2 138 1.31e-13 71.2
MS.gene032176.t1 AT2G32250 31.915 141 88 3 68 204 2 138 1.31e-13 71.2
MS.gene032176.t1 AT2G32250 31.915 141 88 3 68 204 2 138 1.34e-13 71.2
MS.gene032176.t1 AT2G32250 31.915 141 88 3 68 204 2 138 1.34e-13 71.2
MS.gene032176.t1 AT5G28530 31.707 123 78 4 85 202 38 159 1.80e-12 67.8
MS.gene032176.t1 AT5G28530 31.707 123 78 4 85 202 38 159 1.80e-12 67.8
MS.gene032176.t1 AT5G28530 31.707 123 78 4 85 202 38 159 1.82e-12 67.8
MS.gene032176.t1 AT5G28530 31.707 123 78 4 85 202 38 159 2.11e-12 67.8
MS.gene032176.t1 AT5G28530 31.707 123 78 4 85 202 38 159 2.14e-12 67.4
MS.gene032176.t1 AT1G10240 31.579 152 79 7 65 202 14 154 1.93e-11 64.7
MS.gene032176.t1 AT1G10240 31.579 152 79 7 65 202 14 154 1.93e-11 64.7
MS.gene032176.t1 AT3G22170 30.137 146 85 4 77 207 46 189 2.18e-11 64.7
MS.gene032176.t1 AT3G22170 30.137 146 85 4 77 207 46 189 2.18e-11 64.7
MS.gene032176.t1 AT2G32250 35.000 100 59 2 107 204 2 97 3.18e-11 64.3
MS.gene032176.t1 AT2G32250 35.000 100 59 2 107 204 2 97 3.30e-11 63.9

Find 64 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTAATGCCTCACCATTAC+TGG 0.248927 2.3:-4533643 None:intergenic
AGGCGTGATGGATCTGTTAT+TGG 0.276667 2.3:+4533951 MS.gene032176:CDS
GTAATAACCATCTGGAATTC+TGG 0.278538 2.3:+4532958 MS.gene032176:CDS
TGGTTGCAGGGCAATGATTA+TGG 0.280378 2.3:+4534058 MS.gene032176:CDS
CAGAAACAAGGGTTGGTTGC+AGG 0.300475 2.3:+4534045 MS.gene032176:CDS
CAGGAACTATTGAGGTGATT+TGG 0.320322 2.3:-4533791 None:intergenic
TCTTTCTTCTATGTTTGAGC+TGG 0.327573 2.3:+4532894 None:intergenic
AGCGAGCTGAAACCTATAAA+AGG 0.338658 2.3:+4534947 MS.gene032176:CDS
GTTCTCATAACCGATGTTAC+AGG 0.348049 2.3:-4533810 None:intergenic
AAGTCCCTTCTGCCTCTTCC+AGG 0.350515 2.3:-4534149 None:intergenic
AATATGACTCAAGATTCTTC+TGG 0.372511 2.3:+4533762 MS.gene032176:CDS
TTTCGATCCAACTGATGAAC+AGG 0.398044 2.3:+4533728 MS.gene032176:CDS
GCATTAGCAAATGCTACTAT+TGG 0.399597 2.3:+4533657 MS.gene032176:CDS
GGAAATTAAATTCTGGTCTA+TGG 0.402271 2.3:+4534084 MS.gene032176:CDS
AGTAGAGAGTGTGAAGGAGA+TGG 0.412908 2.3:+4535038 MS.gene032176:CDS
ATGGTGAGGAAATTAAATTC+TGG 0.421065 2.3:+4534077 MS.gene032176:CDS
ATGGTTATTACTGAGTTATA+AGG 0.427405 2.3:-4532946 None:intergenic
ACAAGAGGGTCAGTTTCAAT+TGG 0.447254 2.3:+4535071 MS.gene032176:CDS
GGCGTGATGGATCTGTTATT+GGG 0.455689 2.3:+4533952 MS.gene032176:CDS
CTTTCTTCTATGTTTGAGCT+GGG 0.461929 2.3:+4532895 None:intergenic
CTTCGTTTATGAGCAATATC+CGG 0.471781 2.3:+4534169 MS.gene032176:CDS
GCTCTAGTTTGTAACAAAGA+AGG 0.478666 2.3:+4533978 MS.gene032176:CDS
ATAACTCAGTAATAACCATC+TGG 0.481933 2.3:+4532950 MS.gene032176:CDS
AAATGCATGTGCTTCAGCTT+CGG 0.486804 2.3:-4533868 None:intergenic
GGTGCAGTGGAGATTGAGAT+TGG 0.489609 2.3:+4533615 MS.gene032176:intron
AATTCATATGCTACACGTGT+TGG 0.492545 2.3:+4533894 MS.gene032176:CDS
TGATGGATCTGTTATTGGGC+GGG 0.492905 2.3:+4533956 MS.gene032176:CDS
TCTTGATCCTGTTCATCAGT+TGG 0.497177 2.3:-4533735 None:intergenic
AGAAACAAGGGTTGGTTGCA+GGG 0.508414 2.3:+4534046 MS.gene032176:CDS
CTGCCTCTTCCAGGTGTCAG+AGG 0.510409 2.3:-4534140 None:intergenic
CTCAATAGTTCCTGTAACAT+CGG 0.518345 2.3:+4533800 MS.gene032176:CDS
TACAAACTCCTGGCCGATGT+AGG 0.525221 2.3:-4533844 None:intergenic
ACAAGCGGGAAAAGATTGTA+AGG 0.526019 2.3:+4534015 MS.gene032176:CDS
CACACACATCCTCTGACACC+TGG 0.532917 2.3:+4534131 MS.gene032176:CDS
CTCTCAAGATCAAGGCGTGA+TGG 0.535255 2.3:+4533939 MS.gene032176:CDS
ACAAACTCCTGGCCGATGTA+GGG 0.537780 2.3:-4533843 None:intergenic
TTTCAGAATGCCTGACAAGC+GGG 0.539722 2.3:+4534001 MS.gene032176:CDS
CATCCTCTGACACCTGGAAG+AGG 0.555269 2.3:+4534137 MS.gene032176:CDS
GACTCAAGATTCTTCTGGAG+TGG 0.558618 2.3:+4533767 MS.gene032176:CDS
TAAGTAAGCTCTCAAGATCA+AGG 0.559347 2.3:+4533931 MS.gene032176:CDS
TAAGGCAAAGGGCAGAAACA+AGG 0.561852 2.3:+4534033 MS.gene032176:CDS
TCGAGAACTAACTCAACAGT+TGG 0.562574 2.3:+4534912 MS.gene032176:CDS
TGACACCTGGAAGAGGCAGA+AGG 0.566441 2.3:+4534144 MS.gene032176:CDS
TGATGAACCCTACATCGGCC+AGG 0.568038 2.3:+4533836 MS.gene032176:CDS
GGGAAAAGATTGTAAGGCAA+AGG 0.569600 2.3:+4534021 MS.gene032176:CDS
GGAAAAGATTGTAAGGCAAA+GGG 0.570713 2.3:+4534022 MS.gene032176:CDS
CAGCTTCGGATACAAACTCC+TGG 0.571675 2.3:-4533854 None:intergenic
CGATGTTACAGGAACTATTG+AGG 0.587098 2.3:-4533799 None:intergenic
GACACCTGGAAGAGGCAGAA+GGG 0.590498 2.3:+4534145 MS.gene032176:CDS
GATGGAAGCTAAAGAACAAG+AGG 0.602786 2.3:+4535056 MS.gene032176:CDS
TGTACTCATATAACAAGTCA+AGG 0.602934 2.3:-4534972 None:intergenic
GTGATGGATCTGTTATTGGG+CGG 0.611640 2.3:+4533955 MS.gene032176:CDS
CAAAGGGCAGAAACAAGGGT+TGG 0.628129 2.3:+4534038 MS.gene032176:CDS
AGCAAATGCTACTATTGGAG+AGG 0.632541 2.3:+4533662 MS.gene032176:CDS
GTTTCAGAATGCCTGACAAG+CGG 0.633841 2.3:+4534000 MS.gene032176:CDS
GAGATTGGTGACTCCAGTAA+TGG 0.635105 2.3:+4533630 MS.gene032176:CDS
ACAATTGATGAACCCTACAT+CGG 0.643960 2.3:+4533831 MS.gene032176:CDS
TGGTGACTCCAGTAATGGTG+AGG 0.657202 2.3:+4533635 MS.gene032176:CDS
AAGGCAAAGGGCAGAAACAA+GGG 0.671184 2.3:+4534034 MS.gene032176:CDS
ACGGCATCCAGAATTCCAGA+TGG 0.673974 2.3:-4532965 None:intergenic
GCAGGGCAATGATTATGGTG+AGG 0.678028 2.3:+4534063 MS.gene032176:CDS
GCACATAGTAGAGAGTGTGA+AGG 0.682029 2.3:+4535032 MS.gene032176:CDS
ATGGAAGCTAAAGAACAAGA+GGG 0.719285 2.3:+4535057 MS.gene032176:CDS
CTTGTTATATGAGTACATCG+AGG 0.731335 2.3:+4534978 MS.gene032176:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTAATAGATTAATAAATTT+TGG - chr2.3:4533006-4533025 None:intergenic 10.0%
!!! TTTTTTCTTATAAGAAATTA+GGG + chr2.3:4533282-4533301 MS.gene032176:intron 10.0%
!!! TTTTTTTCTTATAAGAAATT+AGG + chr2.3:4533281-4533300 MS.gene032176:intron 10.0%
!! GGGAAATTATTATTAATAAT+CGG + chr2.3:4533308-4533327 MS.gene032176:intron 15.0%
!!! TCTTATATCAAATTTTTTGT+TGG + chr2.3:4533594-4533613 MS.gene032176:intron 15.0%
!!! TCTTTTTCTTTTTTTCATTT+AGG + chr2.3:4533101-4533120 MS.gene032176:intron 15.0%
!!! TTTTAAATAAAGATTTGTAC+AGG - chr2.3:4534281-4534300 None:intergenic 15.0%
!!! TTTTTTTCATTTAGGTTTTT+GGG + chr2.3:4533109-4533128 MS.gene032176:intron 15.0%
!! AAAATATGAAAATAACCATC+TGG + chr2.3:4533041-4533060 MS.gene032176:intron 20.0%
!! AAGTGAAAAGATTAAATGAA+AGG + chr2.3:4534339-4534358 MS.gene032176:intron 20.0%
!! GAAAAAAATTGACAATCTTT+AGG - chr2.3:4533083-4533102 None:intergenic 20.0%
!! TTGGATTTGATAAAACTTAA+CGG - chr2.3:4532987-4533006 None:intergenic 20.0%
!!! AAATATTTAGTGTCATTCAT+AGG + chr2.3:4533361-4533380 MS.gene032176:intron 20.0%
!!! AATATTTAGTGTCATTCATA+GGG + chr2.3:4533362-4533381 MS.gene032176:intron 20.0%
!!! AATTCCAAAATTATCCTTTT+TGG - chr2.3:4533467-4533486 None:intergenic 20.0%
!!! AGTATTTAGTTTAAACCATA+GGG + chr2.3:4533438-4533457 MS.gene032176:intron 20.0%
!!! CTTTTTTTCATTTAGGTTTT+TGG + chr2.3:4533108-4533127 MS.gene032176:intron 20.0%
!!! TAGTATTTAGTTTAAACCAT+AGG + chr2.3:4533437-4533456 MS.gene032176:intron 20.0%
! AAAAAACTATGCCCATTTAA+GGG - chr2.3:4534391-4534410 None:intergenic 25.0%
! AAAAACAAATATGTAAGACG+TGG - chr2.3:4534862-4534881 None:intergenic 25.0%
! AAAACAAATATGTAAGACGT+GGG - chr2.3:4534861-4534880 None:intergenic 25.0%
! AAAATAGGTAAAATTACTCC+CGG + chr2.3:4534641-4534660 MS.gene032176:intron 25.0%
! AAAGAGTGAAAAAAGAGAAA+GGG - chr2.3:4534258-4534277 None:intergenic 25.0%
! AATCCATACACATGAAAAAT+AGG + chr2.3:4534626-4534645 MS.gene032176:intron 25.0%
! AATCTTTAGGTAAAGCTTAA+TGG - chr2.3:4533070-4533089 None:intergenic 25.0%
! AATTCATGTTACATGTCTTT+GGG + chr2.3:4534813-4534832 MS.gene032176:intron 25.0%
! ACCTCGAAGATAAATTAAAA+TGG - chr2.3:4534598-4534617 None:intergenic 25.0%
! ATGCTAACTTAACTAGTAAT+CGG + chr2.3:4533248-4533267 MS.gene032176:intron 25.0%
! ATGGTTATTACTGAGTTATA+AGG - chr2.3:4532949-4532968 None:intergenic 25.0%
! CTGAAACCATTAGAATTTAA+AGG + chr2.3:4534443-4534462 MS.gene032176:intron 25.0%
! GATATAAACATTGAAGTCTT+TGG - chr2.3:4534560-4534579 None:intergenic 25.0%
! GATGGAAATAAAAAACCAAA+AGG + chr2.3:4534365-4534384 MS.gene032176:intron 25.0%
! TAAAAAACTATGCCCATTTA+AGG - chr2.3:4534392-4534411 None:intergenic 25.0%
! TGAGATCCTTTAAATTCTAA+TGG - chr2.3:4534452-4534471 None:intergenic 25.0%
! TTTACAATGAAAAATCATCG+AGG + chr2.3:4534415-4534434 MS.gene032176:intron 25.0%
!! ATTTTGCCAGGTAAATATTT+TGG + chr2.3:4534752-4534771 MS.gene032176:intron 25.0%
!! GTTTCCAAAAAGGATAATTT+TGG + chr2.3:4533460-4533479 MS.gene032176:intron 25.0%
!!! TCCATTTTAATTTATCTTCG+AGG + chr2.3:4534594-4534613 MS.gene032176:intron 25.0%
!!! TTACCTATTTTTCATGTGTA+TGG - chr2.3:4534632-4534651 None:intergenic 25.0%
!!! TTTAAGTTGACTGATCTTTA+TGG + chr2.3:4533147-4533166 MS.gene032176:intron 25.0%
AAACAAATATGTAAGACGTG+GGG - chr2.3:4534860-4534879 None:intergenic 30.0%
AAATAGGTAAAATTACTCCC+GGG + chr2.3:4534642-4534661 MS.gene032176:intron 30.0%
AAGAGTGAAAAAAGAGAAAG+GGG - chr2.3:4534257-4534276 None:intergenic 30.0%
AATATGACTCAAGATTCTTC+TGG + chr2.3:4533762-4533781 MS.gene032176:CDS 30.0%
AGATTAAATGAAAGGAGAGA+TGG + chr2.3:4534347-4534366 MS.gene032176:intron 30.0%
ATAACTCAGTAATAACCATC+TGG + chr2.3:4532950-4532969 MS.gene032176:CDS 30.0%
ATGGTGAGGAAATTAAATTC+TGG + chr2.3:4534077-4534096 MS.gene032176:CDS 30.0%
CAATTCATGTTACATGTCTT+TGG + chr2.3:4534812-4534831 MS.gene032176:intron 30.0%
GAAAGAGTGAAAAAAGAGAA+AGG - chr2.3:4534259-4534278 None:intergenic 30.0%
GGAAATTAAATTCTGGTCTA+TGG + chr2.3:4534084-4534103 MS.gene032176:CDS 30.0%
TCTTATAAGAAATTAGGGCA+GGG + chr2.3:4533287-4533306 MS.gene032176:intron 30.0%
TGAAACATAGTAAAACAGTG+TGG + chr2.3:4534304-4534323 MS.gene032176:intron 30.0%
TGTACTCATATAACAAGTCA+AGG - chr2.3:4534975-4534994 None:intergenic 30.0%
TTCTTATAAGAAATTAGGGC+AGG + chr2.3:4533286-4533305 MS.gene032176:intron 30.0%
! GATTTTGTGCAAAGTCTTTT+TGG + chr2.3:4533218-4533237 MS.gene032176:intron 30.0%
! TTTTTATGCAATTTCCAGAG+TGG + chr2.3:4534877-4534896 MS.gene032176:intron 30.0%
!! ATTAGCAACTTTGACTTGTA+TGG + chr2.3:4533393-4533412 MS.gene032176:intron 30.0%
!! TTTCTAGAACTGCAAATAAC+TGG - chr2.3:4534209-4534228 None:intergenic 30.0%
!!! ATCGGTTTTATTTGTTGAAG+TGG + chr2.3:4533326-4533345 MS.gene032176:intron 30.0%
!!! GTTTTATTTGTTGAAGTGGA+GGG + chr2.3:4533330-4533349 MS.gene032176:intron 30.0%
AAAACCAAAAGGCCCTTAAA+TGG + chr2.3:4534376-4534395 MS.gene032176:intron 35.0%
AAACCAAAAGGCCCTTAAAT+GGG + chr2.3:4534377-4534396 MS.gene032176:intron 35.0%
AAACCATAGGGTTTCCAAAA+AGG + chr2.3:4533450-4533469 MS.gene032176:intron 35.0%
AACAAATATGTAAGACGTGG+GGG - chr2.3:4534859-4534878 None:intergenic 35.0%
AATAGGTAAAATTACTCCCG+GGG + chr2.3:4534643-4534662 MS.gene032176:intron 35.0%
AATTCATATGCTACACGTGT+TGG + chr2.3:4533894-4533913 MS.gene032176:CDS 35.0%
ACAATTGATGAACCCTACAT+CGG + chr2.3:4533831-4533850 MS.gene032176:CDS 35.0%
ATGCACTTAACTTAGAGTGT+TGG + chr2.3:4534670-4534689 MS.gene032176:intron 35.0%
ATGGAAGCTAAAGAACAAGA+GGG + chr2.3:4535057-4535076 MS.gene032176:CDS 35.0%
CAAGTCAAAGTTGCTAATAC+AGG - chr2.3:4533391-4533410 None:intergenic 35.0%
CTCAATAGTTCCTGTAACAT+CGG + chr2.3:4533800-4533819 MS.gene032176:CDS 35.0%
CTGTTGCCAAAATATTTACC+TGG - chr2.3:4534761-4534780 None:intergenic 35.0%
CTTATAAGAAATTAGGGCAG+GGG + chr2.3:4533288-4533307 MS.gene032176:intron 35.0%
CTTCGTTTATGAGCAATATC+CGG + chr2.3:4534169-4534188 MS.gene032176:CDS 35.0%
GCATTAGCAAATGCTACTAT+TGG + chr2.3:4533657-4533676 MS.gene032176:CDS 35.0%
GGAAAAGATTGTAAGGCAAA+GGG + chr2.3:4534022-4534041 MS.gene032176:CDS 35.0%
TAAGTAAGCTCTCAAGATCA+AGG + chr2.3:4533931-4533950 MS.gene032176:CDS 35.0%
TTTGCACAAAATCAAAGCGA+AGG - chr2.3:4533211-4533230 None:intergenic 35.0%
! AAATCGATACTTCGAGTTTC+TGG + chr2.3:4534725-4534744 MS.gene032176:intron 35.0%
! CAAGTCAAGGTTCCTTTTAT+AGG - chr2.3:4534962-4534981 None:intergenic 35.0%
! CTTGTTATATGAGTACATCG+AGG + chr2.3:4534978-4534997 MS.gene032176:CDS 35.0%
! GCTCTAGTTTGTAACAAAGA+AGG + chr2.3:4533978-4533997 MS.gene032176:CDS 35.0%
! GTAATAACCATCTGGAATTC+TGG + chr2.3:4532958-4532977 MS.gene032176:CDS 35.0%
!! AGAAACTCGAAGTATCGATT+TGG - chr2.3:4534726-4534745 None:intergenic 35.0%
!! GAAACTCGAAGTATCGATTT+GGG - chr2.3:4534725-4534744 None:intergenic 35.0%
!! TATCCTTTTTGGAAACCCTA+TGG - chr2.3:4533456-4533475 None:intergenic 35.0%
!! TGCAGTTCTAGAAAAGTGTA+TGG + chr2.3:4534215-4534234 MS.gene032176:intron 35.0%
!!! CAAATTTTTTGTTGGTGCAG+TGG + chr2.3:4533602-4533621 MS.gene032176:intron 35.0%
!!! GGTTTTATTTGTTGAAGTGG+AGG + chr2.3:4533329-4533348 MS.gene032176:intron 35.0%
AAAGCTTAATGGCATCCAGA+TGG - chr2.3:4533059-4533078 None:intergenic 40.0%
AAATGCATGTGCTTCAGCTT+CGG - chr2.3:4533871-4533890 None:intergenic 40.0%
ACAAATATGTAAGACGTGGG+GGG - chr2.3:4534858-4534877 None:intergenic 40.0%
ACAAGAGGGTCAGTTTCAAT+TGG + chr2.3:4535071-4535090 MS.gene032176:CDS 40.0%
ACAAGCGGGAAAAGATTGTA+AGG + chr2.3:4534015-4534034 MS.gene032176:CDS 40.0%
ACTGGTAGAACATGACTAAC+CGG - chr2.3:4534191-4534210 None:intergenic 40.0%
AGCAAATGCTACTATTGGAG+AGG + chr2.3:4533662-4533681 MS.gene032176:CDS 40.0%
AGCGAGCTGAAACCTATAAA+AGG + chr2.3:4534947-4534966 MS.gene032176:CDS 40.0%
ATAGGTAAAATTACTCCCGG+GGG + chr2.3:4534644-4534663 MS.gene032176:intron 40.0%
CAGGAACTATTGAGGTGATT+TGG - chr2.3:4533794-4533813 None:intergenic 40.0%
CGATGTTACAGGAACTATTG+AGG - chr2.3:4533802-4533821 None:intergenic 40.0%
GATGGAAGCTAAAGAACAAG+AGG + chr2.3:4535056-4535075 MS.gene032176:CDS 40.0%
GGGAAAAGATTGTAAGGCAA+AGG + chr2.3:4534021-4534040 MS.gene032176:CDS 40.0%
GTTCTCATAACCGATGTTAC+AGG - chr2.3:4533813-4533832 None:intergenic 40.0%
TCGAGAACTAACTCAACAGT+TGG + chr2.3:4534912-4534931 MS.gene032176:CDS 40.0%
TCTTGATCCTGTTCATCAGT+TGG - chr2.3:4533738-4533757 None:intergenic 40.0%
TTTCGATCCAACTGATGAAC+AGG + chr2.3:4533728-4533747 MS.gene032176:CDS 40.0%
! AGAAAAGTGTATGGTCTGCT+TGG + chr2.3:4534224-4534243 MS.gene032176:intron 40.0%
!!! GTTTCTGGAGTAATTTTGCC+AGG + chr2.3:4534740-4534759 MS.gene032176:intron 40.0%
AAATATGTAAGACGTGGGGG+GGG - chr2.3:4534856-4534875 None:intergenic 45.0%
AAGGCAAAGGGCAGAAACAA+GGG + chr2.3:4534034-4534053 MS.gene032176:CDS 45.0%
AAGTTAAGTGCATAGACCCC+CGG - chr2.3:4534663-4534682 None:intergenic 45.0%
CAAATATGTAAGACGTGGGG+GGG - chr2.3:4534857-4534876 None:intergenic 45.0%
GTTTCAGAATGCCTGACAAG+CGG + chr2.3:4534000-4534019 MS.gene032176:CDS 45.0%
TAAGGCAAAGGGCAGAAACA+AGG + chr2.3:4534033-4534052 MS.gene032176:CDS 45.0%
TGCTAATGCCTCACCATTAC+TGG - chr2.3:4533646-4533665 None:intergenic 45.0%
TGGTTGCAGGGCAATGATTA+TGG + chr2.3:4534058-4534077 MS.gene032176:CDS 45.0%
TTTCAGAATGCCTGACAAGC+GGG + chr2.3:4534001-4534020 MS.gene032176:CDS 45.0%
! ACAATCTTTTCCCGCTTGTC+AGG - chr2.3:4534014-4534033 None:intergenic 45.0%
! AGTAGAGAGTGTGAAGGAGA+TGG + chr2.3:4535038-4535057 MS.gene032176:CDS 45.0%
! ATGCCCATTTAAGGGCCTTT+TGG - chr2.3:4534383-4534402 None:intergenic 45.0%
! GACTCAAGATTCTTCTGGAG+TGG + chr2.3:4533767-4533786 MS.gene032176:CDS 45.0%
! GAGATTGGTGACTCCAGTAA+TGG + chr2.3:4533630-4533649 MS.gene032176:CDS 45.0%
! GCACATAGTAGAGAGTGTGA+AGG + chr2.3:4535032-4535051 MS.gene032176:CDS 45.0%
! TGATGGATCTGTTATTGGGC+GGG + chr2.3:4533956-4533975 MS.gene032176:CDS 45.0%
!! AGAAACAAGGGTTGGTTGCA+GGG + chr2.3:4534046-4534065 MS.gene032176:CDS 45.0%
!! AGGCGTGATGGATCTGTTAT+TGG + chr2.3:4533951-4533970 MS.gene032176:CDS 45.0%
!! GGCGTGATGGATCTGTTATT+GGG + chr2.3:4533952-4533971 MS.gene032176:CDS 45.0%
!! GTGATGGATCTGTTATTGGG+CGG + chr2.3:4533955-4533974 MS.gene032176:CDS 45.0%
AATATGTAAGACGTGGGGGG+GGG - chr2.3:4534855-4534874 None:intergenic 50.0%
ACAAACTCCTGGCCGATGTA+GGG - chr2.3:4533846-4533865 None:intergenic 50.0%
ACGGCATCCAGAATTCCAGA+TGG - chr2.3:4532968-4532987 None:intergenic 50.0%
AGTTAAGTGCATAGACCCCC+GGG - chr2.3:4534662-4534681 None:intergenic 50.0%
CAAAGGGCAGAAACAAGGGT+TGG + chr2.3:4534038-4534057 MS.gene032176:CDS 50.0%
CAGCTTCGGATACAAACTCC+TGG - chr2.3:4533857-4533876 None:intergenic 50.0%
CCGAGTGATACTCTACAGAG+CGG - chr2.3:4533565-4533584 None:intergenic 50.0%
CTCTCAAGATCAAGGCGTGA+TGG + chr2.3:4533939-4533958 MS.gene032176:CDS 50.0%
GCAGGGCAATGATTATGGTG+AGG + chr2.3:4534063-4534082 MS.gene032176:CDS 50.0%
GGTGCAGTGGAGATTGAGAT+TGG + chr2.3:4533615-4533634 MS.gene032176:intron 50.0%
TACAAACTCCTGGCCGATGT+AGG - chr2.3:4533847-4533866 None:intergenic 50.0%
TGGTGACTCCAGTAATGGTG+AGG + chr2.3:4533635-4533654 MS.gene032176:CDS 50.0%
! CCGCTCTGTAGAGTATCACT+CGG + chr2.3:4533562-4533581 MS.gene032176:intron 50.0%
!! CAGAAACAAGGGTTGGTTGC+AGG + chr2.3:4534045-4534064 MS.gene032176:CDS 50.0%
AAGTCCCTTCTGCCTCTTCC+AGG - chr2.3:4534152-4534171 None:intergenic 55.0%
ATATGTAAGACGTGGGGGGG+GGG - chr2.3:4534854-4534873 None:intergenic 55.0%
CACACACATCCTCTGACACC+TGG + chr2.3:4534131-4534150 MS.gene032176:CDS 55.0%
CATCCTCTGACACCTGGAAG+AGG + chr2.3:4534137-4534156 MS.gene032176:CDS 55.0%
GAACTCTGTCGTGTCCACTC+TGG - chr2.3:4534894-4534913 None:intergenic 55.0%
GACACCTGGAAGAGGCAGAA+GGG + chr2.3:4534145-4534164 MS.gene032176:CDS 55.0%
TGACACCTGGAAGAGGCAGA+AGG + chr2.3:4534144-4534163 MS.gene032176:CDS 55.0%
TGATGAACCCTACATCGGCC+AGG + chr2.3:4533836-4533855 MS.gene032176:CDS 55.0%
CTGCCTCTTCCAGGTGTCAG+AGG - chr2.3:4534143-4534162 None:intergenic 60.0%
TATGTAAGACGTGGGGGGGG+GGG - chr2.3:4534853-4534872 None:intergenic 60.0%
ATGTAAGACGTGGGGGGGGG+GGG - chr2.3:4534852-4534871 None:intergenic 65.0%
GGGGGGGGGGGACTGAAAAG+GGG - chr2.3:4534840-4534859 None:intergenic 70.0%
GGGGGGGGGGGGACTGAAAA+GGG - chr2.3:4534841-4534860 None:intergenic 70.0%
TGGGGGGGGGGGGACTGAAA+AGG - chr2.3:4534842-4534861 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 4532904 4535104 4532904 ID=MS.gene032176
chr2.3 mRNA 4532904 4535104 4532904 ID=MS.gene032176.t1;Parent=MS.gene032176
chr2.3 exon 4532904 4532985 4532904 ID=MS.gene032176.t1.exon1;Parent=MS.gene032176.t1
chr2.3 CDS 4532904 4532985 4532904 ID=cds.MS.gene032176.t1;Parent=MS.gene032176.t1
chr2.3 exon 4533622 4534190 4533622 ID=MS.gene032176.t1.exon2;Parent=MS.gene032176.t1
chr2.3 CDS 4533622 4534190 4533622 ID=cds.MS.gene032176.t1;Parent=MS.gene032176.t1
chr2.3 exon 4534895 4535104 4534895 ID=MS.gene032176.t1.exon3;Parent=MS.gene032176.t1
chr2.3 CDS 4534895 4535104 4534895 ID=cds.MS.gene032176.t1;Parent=MS.gene032176.t1
Gene Sequence

>MS.gene032176

ATGTTTGAGCTGGGAGAAATCATGAAGAACATATTTTTGAGACCTTATAACTCAGTAATAACCATCTGGAATTCTGGATGCCGTTAAGTTTTATCAAATCCAAAATTTATTAATCTATTAAGATTAAGTTTTTTTCAAAAATATGAAAATAACCATCTGGATGCCATTAAGCTTTACCTAAAGATTGTCAATTTTTTTCTTTTTCTTTTTTTCATTTAGGTTTTTGGGCTTTGTTATATTTGTTTTAAGTTGACTGATCTTTATGGCATGTTTGTTGTTGTACTTCAGAAATATGTGAATTTTTCCTTCGCTTTGATTTTGTGCAAAGTCTTTTTGGATTCAGCATGCTAACTTAACTAGTAATCGGTTTTTTTTTTTTTTTTTCTTATAAGAAATTAGGGCAGGGGAAATTATTATTAATAATCGGTTTTATTTGTTGAAGTGGAGGGTATTTGTCAAATATTTAGTGTCATTCATAGGGTTTCCTGTATTAGCAACTTTGACTTGTATGGTTTTCTCTCTCTGATTCATTGTAGTATTTAGTTTAAACCATAGGGTTTCCAAAAAGGATAATTTTGGAATTAATACGATATGTTTCTTCTTCAGTTCTTTCATAATTTTTATCATCACTGAAGCAGCATTTCATCTATTTTATACACCGCTCTGTAGAGTATCACTCGGCAATGAAAGTCTTATATCAAATTTTTTGTTGGTGCAGTGGAGATTGAGATTGGTGACTCCAGTAATGGTGAGGCATTAGCAAATGCTACTATTGGAGAGGTCAATAGTAGCATAGCTGACAAAGAAATAATTGAATGCAGTTCTTTCGATCCAACTGATGAACAGGATCAAGATGATAATATGACTCAAGATTCTTCTGGAGTGGACCAAATCACCTCAATAGTTCCTGTAACATCGGTTATGAGAACAATTGATGAACCCTACATCGGCCAGGAGTTTGTATCCGAAGCTGAAGCACATGCATTTTATAATTCATATGCTACACGTGTTGGATTCGTCATACGTGTAAGTAAGCTCTCAAGATCAAGGCGTGATGGATCTGTTATTGGGCGGGCTCTAGTTTGTAACAAAGAAGGTTTCAGAATGCCTGACAAGCGGGAAAAGATTGTAAGGCAAAGGGCAGAAACAAGGGTTGGTTGCAGGGCAATGATTATGGTGAGGAAATTAAATTCTGGTCTATGGTCTATAACAAAGTTTGTAAAAGAGCACACACATCCTCTGACACCTGGAAGAGGCAGAAGGGACTTCGTTTATGAGCAATATCCGGTTAGTCATGTTCTACCAGTTATTTGCAGTTCTAGAAAAGTGTATGGTCTGCTTGGTTTATGTCCCCTTTCTCTTTTTTCACTCTTTCCTGTACAAATCTTTATTTAAAAACATGAAACATAGTAAAACAGTGTGGCATTTTTATTAAAAGTGAAAAGATTAAATGAAAGGAGAGATGGAAATAAAAAACCAAAAGGCCCTTAAATGGGCATAGTTTTTTATTTTTTACAATGAAAAATCATCGAGGTACTTCTGAAACCATTAGAATTTAAAGGATCTCATCACTGATTTTTATTAAGATGTTCATCAATCATATTTGTAGTACTGTTTATAACTTACACTTGTTTATGCAAGAAGAGAAACTAACCAAAGACTTCAATGTTTATATCTATGCTTATTGATTTCCATTTTAATTTATCTTCGAGGTTCTGTATCAATCCATACACATGAAAAATAGGTAAAATTACTCCCGGGGGTCTATGCACTTAACTTAGAGTGTTGGATTAATTCTTTAACTCTCTTTTTTATCTTCCCAAATCGATACTTCGAGTTTCTGGAGTAATTTTGCCAGGTAAATATTTTGGCAACAGCAAATTGATACTTATGTACAAGTAACTTGAGCAATTCATGTTACATGTCTTTGGGACCCCTTTTCAGTCCCCCCCCCCCCACGTCTTACATATTTGTTTTTATGCAATTTCCAGAGTGGACACGACAGAGTTCGAGAACTAACTCAACAGTTGGCTATAGAGAAAAAGCGAGCTGAAACCTATAAAAGGAACCTTGACTTGTTATATGAGTACATCGAGGAGCATAATGAAGCTGTTTCAAAGAAGATACAGCACATAGTAGAGAGTGTGAAGGAGATGGAAGCTAAAGAACAAGAGGGTCAGTTTCAATTGGCTGTTATCTAG
Protein sequence

>MS.gene032176.t1

MFELGEIMKNIFLRPYNSVITIWNSGCLEIEIGDSSNGEALANATIGEVNSSIADKEIIECSSFDPTDEQDQDDNMTQDSSGVDQITSIVPVTSVMRTIDEPYIGQEFVSEAEAHAFYNSYATRVGFVIRVSKLSRSRRDGSVIGRALVCNKEGFRMPDKREKIVRQRAETRVGCRAMIMVRKLNSGLWSITKFVKEHTHPLTPGRGRRDFVYEQYPSGHDRVRELTQQLAIEKKRAETYKRNLDLLYEYIEEHNEAVSKKIQHIVESVKEMEAKEQEGQFQLAVI