AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032215

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032215.t1 MTR_2g099490 94.813 347 16 1 1 345 1 347 0.0 655
MS.gene032215.t1 MTR_2g099490 91.304 345 16 1 1 345 1 331 0.0 624
MS.gene032215.t1 MTR_8g019540 51.471 340 120 12 23 345 7 318 8.83e-85 259
MS.gene032215.t1 MTR_2g041090 55.147 136 49 4 121 253 19 145 7.93e-40 140
MS.gene032215.t1 MTR_2g030170 52.174 115 51 2 168 282 95 205 5.00e-33 122
MS.gene032215.t1 MTR_2g030170 52.174 115 51 2 168 282 128 238 2.20e-32 121
MS.gene032215.t1 MTR_8g037270 56.311 103 40 1 144 246 117 214 1.16e-30 118
MS.gene032215.t1 MTR_3g061510 49.624 133 52 3 114 246 112 229 1.58e-30 118
MS.gene032215.t1 MTR_3g061510 49.624 133 52 3 114 246 132 249 2.00e-30 119
MS.gene032215.t1 MTR_7g106450 51.000 100 40 3 147 246 136 226 4.86e-24 100
MS.gene032215.t1 MTR_1g056530 41.844 141 67 4 108 245 95 223 6.27e-24 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032215.t1 AT1G17590 38.028 355 163 13 10 345 12 328 1.44e-52 177
MS.gene032215.t1 AT1G17590 38.028 355 163 13 10 345 12 328 1.44e-52 177
MS.gene032215.t1 AT1G17590 38.028 355 163 13 10 345 12 328 1.44e-52 177
MS.gene032215.t1 AT1G17590 38.028 355 163 13 10 345 12 328 1.44e-52 177
MS.gene032215.t1 AT1G72830 36.826 334 169 11 2 318 4 312 1.64e-49 169
MS.gene032215.t1 AT1G72830 36.716 335 169 12 2 318 4 313 8.93e-48 164
MS.gene032215.t1 AT1G54160 38.281 256 107 8 22 250 11 242 1.04e-39 142
MS.gene032215.t1 AT3G14020 37.690 329 134 16 20 318 13 300 1.66e-39 142
MS.gene032215.t1 AT3G14020 37.690 329 134 16 20 318 13 300 1.66e-39 142
MS.gene032215.t1 AT1G72830 34.535 333 153 11 2 318 4 287 8.23e-38 137
MS.gene032215.t1 AT1G30500 51.938 129 58 2 130 254 40 168 1.05e-36 131
MS.gene032215.t1 AT1G30500 52.381 126 58 2 130 254 40 164 2.02e-36 130
MS.gene032215.t1 AT2G34720 51.613 124 51 3 123 246 44 158 6.68e-33 121
MS.gene032215.t1 AT2G34720 52.941 119 47 3 128 246 14 123 1.62e-31 117
MS.gene032215.t1 AT2G34720 52.941 119 47 3 128 246 14 123 1.62e-31 117
MS.gene032215.t1 AT3G20910 45.926 135 59 4 117 251 111 231 1.48e-29 115
MS.gene032215.t1 AT5G12840 47.015 134 55 4 116 248 116 234 6.59e-29 113
MS.gene032215.t1 AT5G12840 47.015 134 55 4 116 248 116 234 6.59e-29 113
MS.gene032215.t1 AT5G12840 47.015 134 55 4 116 248 116 234 6.59e-29 113
MS.gene032215.t1 AT5G12840 47.015 134 55 4 116 248 115 233 6.80e-29 113
MS.gene032215.t1 AT5G12840 47.015 134 55 4 116 248 115 233 6.80e-29 113
MS.gene032215.t1 AT5G06510 48.214 112 46 4 149 259 9 109 5.40e-24 97.1
MS.gene032215.t1 AT5G06510 48.214 112 46 4 149 259 9 109 5.40e-24 97.1
MS.gene032215.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.38e-23 98.6
MS.gene032215.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.38e-23 98.6
MS.gene032215.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.38e-23 98.6
MS.gene032215.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.38e-23 98.6
MS.gene032215.t1 AT5G06510 47.788 113 47 4 149 260 55 156 2.42e-23 96.7
MS.gene032215.t1 AT3G05690 37.500 168 86 6 85 245 44 199 6.95e-20 89.0
MS.gene032215.t1 AT3G05690 37.500 168 86 6 85 245 81 236 9.26e-20 89.0

Find 80 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTTGAATTCACATATA+TGG 0.180422 2.3:+4150139 None:intergenic
CACTTTGAAGCTCAAACTTT+TGG 0.192638 2.3:+4151827 None:intergenic
TTCAACCTGATCATGCATTT+TGG 0.229189 2.3:+4149784 None:intergenic
GCGAATCAAGCTTTGATTCT+TGG 0.254868 2.3:+4149873 None:intergenic
TTCATATGGCCAACAATATA+AGG 0.305273 2.3:-4150703 MS.gene032215:intron
TATAGCTGGTGGAAACATTT+TGG 0.306979 2.3:+4149840 None:intergenic
GAGAACCGAGGAGAACAATA+TGG 0.321909 2.3:-4150944 MS.gene032215:CDS
AAATTCTGACCAGCTTCTTC+AGG 0.334629 2.3:-4150222 MS.gene032215:CDS
CTCATCGAATCATCATCTAT+TGG 0.336945 2.3:-4150916 MS.gene032215:CDS
TGCTTTGAATTCACATATAT+GGG 0.338735 2.3:+4150140 None:intergenic
TTCCAGCATTGTTCTTCTAC+TGG 0.343547 2.3:-4150979 MS.gene032215:CDS
GGAGGATGGACGGTGAGATT+TGG 0.358180 2.3:+4150889 None:intergenic
AATTCCCTTGCCATCTGATA+TGG 0.360890 2.3:-4150171 MS.gene032215:CDS
GCTTCTTCAGGGCCAGCTAA+TGG 0.362306 2.3:-4150210 MS.gene032215:CDS
GGCCTACTTTCTGCTTCATA+TGG 0.362322 2.3:-4150717 MS.gene032215:CDS
CACCGTCCATCCTCCACTAA+TGG 0.364359 2.3:-4150881 MS.gene032215:CDS
TATACTTGTTTGAATTTGAA+TGG 0.374199 2.3:-4149821 MS.gene032215:CDS
TATCAGATGGCAAGGGAATT+CGG 0.376992 2.3:+4150174 None:intergenic
TACAAATTACCTTATATTGT+TGG 0.397767 2.3:+4150694 None:intergenic
TCATCGAATCATCATCTATT+GGG 0.410536 2.3:-4150915 MS.gene032215:CDS
CAGAAGCAGTTTCCATTAGC+TGG 0.410914 2.3:+4150198 None:intergenic
TGTCGGGACTCGTGAAGATA+TGG 0.413042 2.3:+4149956 None:intergenic
TATATCTGCTTACAAGTGAT+TGG 0.427209 2.3:+4150851 None:intergenic
AGCACATAACAAACTCATAA+AGG 0.430383 2.3:-4150072 MS.gene032215:CDS
TGAAATGTTTCAGCAGCAAC+AGG 0.453392 2.3:-4149714 MS.gene032215:CDS
ATGTCACATACGGATCTCAT+AGG 0.460134 2.3:-4149739 MS.gene032215:CDS
TTCCTTCCAGTCTGAGAAGA+AGG 0.473570 2.3:+4149665 None:intergenic
AATTCTGACCAGCTTCTTCA+GGG 0.474643 2.3:-4150221 MS.gene032215:CDS
AGCAACAGGAGTTAGAGTTC+AGG 0.485902 2.3:-4149700 MS.gene032215:CDS
CAAATTCAAACAAGTATAGC+TGG 0.488784 2.3:+4149826 None:intergenic
GAATTTGAATGGAAATATGT+CGG 0.489962 2.3:-4149810 MS.gene032215:CDS
GGAATCCAAAATGCATGATC+AGG 0.490416 2.3:-4149789 MS.gene032215:CDS
CTTTAATGCATGTACATGTC+GGG 0.500813 2.3:+4149940 None:intergenic
TCGACTGGCTCATCACAATC+AGG 0.502357 2.3:-4151726 MS.gene032215:intron
GAAAGTAGGCCATTGTAGCC+TGG 0.505229 2.3:+4150729 None:intergenic
AACCAGTAGAAGAACAATGC+TGG 0.515161 2.3:+4150977 None:intergenic
TATGAGGCGAAGACAGTGTC+GGG 0.515554 2.3:-4150105 MS.gene032215:CDS
GCACTCCACTATGCTGATCC+AGG 0.517924 2.3:-4150747 MS.gene032215:CDS
TCAGAAGATCTTCCCCATGA+TGG 0.517971 2.3:+4151876 None:intergenic
AGCAGTACCATGCTATTATG+AGG 0.519657 2.3:-4150121 MS.gene032215:CDS
TCTTTAATGCATGTACATGT+CGG 0.521057 2.3:+4149939 None:intergenic
AGAACCGAGGAGAACAATAT+GGG 0.525995 2.3:-4150943 MS.gene032215:CDS
AACTCAGTGCTTTACTGTTG+TGG 0.539967 2.3:+4151784 None:intergenic
AGCATTGAGAAAACGGCCAC+CGG 0.542799 2.3:+4149904 None:intergenic
ACATCCCATATTGTTCTCCT+CGG 0.546379 2.3:+4150939 None:intergenic
CAAAAGTTTGAGCTTCAAAG+TGG 0.551748 2.3:-4151826 MS.gene032215:CDS
TTCCGCCATATCAGATGGCA+AGG 0.552797 2.3:+4150166 None:intergenic
TTAGCTGGCCCTGAAGAAGC+TGG 0.554264 2.3:+4150213 None:intergenic
GTAGAAGAACAATGCTGGAA+AGG 0.555226 2.3:+4150982 None:intergenic
TTAAAGAGAGCTAGAGGTGC+CGG 0.556911 2.3:-4149923 MS.gene032215:CDS
TCATCTTCAACTCAATCGAC+TGG 0.564458 2.3:-4151741 MS.gene032215:CDS
GATTGGCTGTGATCCATTAG+TGG 0.569834 2.3:+4150868 None:intergenic
ACCCTTCTTCTCAGACTGGA+AGG 0.577435 2.3:-4149667 MS.gene032215:CDS
CTCACATGCGGACGGATAGT+CGG 0.579968 2.3:+4149597 None:intergenic
TGTAGCCTGGATCAGCATAG+TGG 0.581932 2.3:+4150742 None:intergenic
ATGATGGACATCCGAAAACA+TGG 0.581989 2.3:+4151892 None:intergenic
GATGCTTCTGATGTCACATA+CGG 0.584773 2.3:-4149749 MS.gene032215:CDS
TATGCTGATCCAGGCTACAA+TGG 0.586886 2.3:-4150738 MS.gene032215:CDS
GGTTCTTCCGCCATATCAGA+TGG 0.587888 2.3:+4150161 None:intergenic
TCCCTTGCCATCTGATATGG+CGG 0.605293 2.3:-4150168 MS.gene032215:CDS
AGTTACCCTTCTTCTCAGAC+TGG 0.606122 2.3:-4149671 MS.gene032215:CDS
ATTCAAACAAGTATAGCTGG+TGG 0.610080 2.3:+4149829 None:intergenic
GGCCATATGAAGCAGAAAGT+AGG 0.615452 2.3:+4150715 None:intergenic
TACAAAGCTGACAAGAATGT+TGG 0.616527 2.3:-4149632 MS.gene032215:CDS
TTATGAGGCGAAGACAGTGT+CGG 0.619331 2.3:-4150106 MS.gene032215:CDS
ATCCATTAGTGGAGGATGGA+CGG 0.629213 2.3:+4150879 None:intergenic
TTACTGTTGTGGCATCCTTG+TGG 0.631295 2.3:+4151795 None:intergenic
ACAAGTCTCTCACATGCGGA+CGG 0.640777 2.3:+4149589 None:intergenic
GTCTTCGCCTCATAATAGCA+TGG 0.641090 2.3:+4150114 None:intergenic
TCCTTCCAGTCTGAGAAGAA+GGG 0.646710 2.3:+4149666 None:intergenic
CATGCATTAAAGAGAGCTAG+AGG 0.647089 2.3:-4149929 MS.gene032215:CDS
TTCTACAAGTCTCTCACATG+CGG 0.648923 2.3:+4149585 None:intergenic
TGTGATCCATTAGTGGAGGA+TGG 0.649182 2.3:+4150875 None:intergenic
ATGGACATCCGAAAACATGG+TGG 0.675905 2.3:+4151895 None:intergenic
TCCGCCATATCAGATGGCAA+GGG 0.679431 2.3:+4150167 None:intergenic
AAGAGAGCTAGAGGTGCCGG+TGG 0.680129 2.3:-4149920 MS.gene032215:CDS
TTCAACATTTAAGAGAACCG+AGG 0.691304 2.3:-4150956 MS.gene032215:CDS
TGGCTGTGATCCATTAGTGG+AGG 0.701564 2.3:+4150871 None:intergenic
AAAGTGGAAGCTCTACCACA+AGG 0.704797 2.3:-4151810 MS.gene032215:CDS
TGGACATCCGAAAACATGGT+GGG 0.707024 2.3:+4151896 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAAAACTTAAATTATA+TGG + chr2.3:4150223-4150242 None:intergenic 10.0%
!!! TTAAGTTTTTCTTATAAAAT+TGG - chr2.3:4150229-4150248 MS.gene032215:CDS 10.0%
!!! TTTTTTATCTCTTTTAATTA+CGG - chr2.3:4150870-4150889 MS.gene032215:CDS 10.0%
!!! AATTATTTTGATTTCTTATG+AGG - chr2.3:4151205-4151224 MS.gene032215:intron 15.0%
!!! TATTTACAAGTTTTTTTTGA+AGG + chr2.3:4150996-4151015 None:intergenic 15.0%
!!! TCTATTTCTTAAATTATTAC+TGG - chr2.3:4150044-4150063 MS.gene032215:intron 15.0%
!! AAAACTTGTAAATAGATGTT+AGG - chr2.3:4151002-4151021 MS.gene032215:intron 20.0%
!! TACAAATTACCTTATATTGT+TGG + chr2.3:4150843-4150862 None:intergenic 20.0%
!! TATACTTGTTTGAATTTGAA+TGG - chr2.3:4151713-4151732 MS.gene032215:intron 20.0%
!! TATATATATCTATGTTCTAG+TGG - chr2.3:4150702-4150721 MS.gene032215:intron 20.0%
!!! TTCTCATTTTGTTATGTTAT+TGG - chr2.3:4149882-4149901 MS.gene032215:CDS 20.0%
! AAATTGGAATTTGTCTTAGA+TGG - chr2.3:4150245-4150264 MS.gene032215:CDS 25.0%
! AGTGCAAATACTAATCAAAT+GGG + chr2.3:4150510-4150529 None:intergenic 25.0%
! CTATATATTGGTAAAAGCTA+TGG - chr2.3:4150193-4150212 MS.gene032215:CDS 25.0%
! GAATTTGAATGGAAATATGT+CGG - chr2.3:4151724-4151743 MS.gene032215:intron 25.0%
! TAATATCAATATCAACATGC+TGG + chr2.3:4150920-4150939 None:intergenic 25.0%
! TGCTGAATTACTTAATTTAG+TGG - chr2.3:4150475-4150494 MS.gene032215:intron 25.0%
!! AGACACTTTTCTCAAAATTT+TGG - chr2.3:4150748-4150767 MS.gene032215:CDS 25.0%
!! GACACTTTTCTCAAAATTTT+GGG - chr2.3:4150749-4150768 MS.gene032215:CDS 25.0%
!! TCAATTTTCAGTCATAACTA+TGG + chr2.3:4151253-4151272 None:intergenic 25.0%
!! TGCTTTGAATTCACATATAT+GGG + chr2.3:4151397-4151416 None:intergenic 25.0%
AGCACATAACAAACTCATAA+AGG - chr2.3:4151462-4151481 MS.gene032215:intron 30.0%
ATGATTTATGAAAGCCGTAA+AGG + chr2.3:4151121-4151140 None:intergenic 30.0%
CAAATTCAAACAAGTATAGC+TGG + chr2.3:4151711-4151730 None:intergenic 30.0%
CAGTGCAAATACTAATCAAA+TGG + chr2.3:4150511-4150530 None:intergenic 30.0%
CCATTTAGAGGAACAAATTA+AGG + chr2.3:4151061-4151080 None:intergenic 30.0%
GTGCAAATACTAATCAAATG+GGG + chr2.3:4150509-4150528 None:intergenic 30.0%
TACTACTGCAGTTTCTTAAT+AGG + chr2.3:4149919-4149938 None:intergenic 30.0%
TATATCTATGTTCTAGTGGA+TGG - chr2.3:4150706-4150725 MS.gene032215:CDS 30.0%
TATATCTGCTTACAAGTGAT+TGG + chr2.3:4150686-4150705 None:intergenic 30.0%
TCATCGAATCATCATCTATT+GGG - chr2.3:4150619-4150638 MS.gene032215:intron 30.0%
TCTTTAATGCATGTACATGT+CGG + chr2.3:4151598-4151617 None:intergenic 30.0%
TGCAAATACTAATCAAATGG+GGG + chr2.3:4150508-4150527 None:intergenic 30.0%
TTCATATGGCCAACAATATA+AGG - chr2.3:4150831-4150850 MS.gene032215:intron 30.0%
TTGATGGGAAAATAAATGGT+TGG - chr2.3:4150379-4150398 MS.gene032215:intron 30.0%
! ATCGTTGATGGGAAAATAAA+TGG - chr2.3:4150375-4150394 MS.gene032215:intron 30.0%
! CTGCTTTGAATTCACATATA+TGG + chr2.3:4151398-4151417 None:intergenic 30.0%
! GAGTTTTCAATTTCAAGAAC+AGG - chr2.3:4149768-4149787 MS.gene032215:CDS 30.0%
!! ACACCTTTAATTTGAAGTGT+CGG + chr2.3:4149960-4149979 None:intergenic 30.0%
!! CCTTAATTTGTTCCTCTAAA+TGG - chr2.3:4151058-4151077 MS.gene032215:intron 30.0%
!! GCTTTTTAGCATTGAGAAAA+CGG + chr2.3:4151640-4151659 None:intergenic 30.0%
!! TATAAATTGACTCGCTTATG+AGG + chr2.3:4150333-4150352 None:intergenic 30.0%
!!! TTCTCAAAATTTTGGGTTTC+AGG - chr2.3:4150756-4150775 MS.gene032215:CDS 30.0%
AACAATATTGTTAGTGCGGT+TGG + chr2.3:4151552-4151571 None:intergenic 35.0%
ACACATACACAGCAGATAAT+GGG + chr2.3:4150417-4150436 None:intergenic 35.0%
AGTTATGAAGTAACGTGTCA+AGG + chr2.3:4150292-4150311 None:intergenic 35.0%
ATTATGTAAACCTACCTCCT+TGG - chr2.3:4150160-4150179 MS.gene032215:CDS 35.0%
ATTCAAACAAGTATAGCTGG+TGG + chr2.3:4151708-4151727 None:intergenic 35.0%
CAAAAGTTTGAGCTTCAAAG+TGG - chr2.3:4149708-4149727 MS.gene032215:CDS 35.0%
CAAGATATCTCATCGTTGAT+GGG - chr2.3:4150364-4150383 MS.gene032215:intron 35.0%
CTCATCGAATCATCATCTAT+TGG - chr2.3:4150618-4150637 MS.gene032215:intron 35.0%
CTTTAATGCATGTACATGTC+GGG + chr2.3:4151597-4151616 None:intergenic 35.0%
GATTAGTATTTGCACTGTGT+TGG - chr2.3:4150514-4150533 MS.gene032215:intron 35.0%
GCACAACAATATTGTTAGTG+CGG + chr2.3:4151556-4151575 None:intergenic 35.0%
TACAAAGCTGACAAGAATGT+TGG - chr2.3:4151902-4151921 MS.gene032215:CDS 35.0%
TACACATACACAGCAGATAA+TGG + chr2.3:4150418-4150437 None:intergenic 35.0%
TCGAATGTAACTATGTGAGT+CGG + chr2.3:4150019-4150038 None:intergenic 35.0%
TGATTGAACAGTCCATTTAG+AGG + chr2.3:4151073-4151092 None:intergenic 35.0%
TTCAACATTTAAGAGAACCG+AGG - chr2.3:4150578-4150597 MS.gene032215:intron 35.0%
! TATAGCTGGTGGAAACATTT+TGG + chr2.3:4151697-4151716 None:intergenic 35.0%
! TGCATATATTTGAGACCAGA+GGG - chr2.3:4151165-4151184 MS.gene032215:intron 35.0%
! TTCAACCTGATCATGCATTT+TGG + chr2.3:4151753-4151772 None:intergenic 35.0%
! TTGCATATATTTGAGACCAG+AGG - chr2.3:4151164-4151183 MS.gene032215:intron 35.0%
! TTTTGGACATGGATGTTTGT+TGG + chr2.3:4149693-4149712 None:intergenic 35.0%
!! CTGTTCTTCATAGCTTTTTC+TGG - chr2.3:4150097-4150116 MS.gene032215:CDS 35.0%
!! CTTCAAATTAAAGGTGTCTC+CGG - chr2.3:4149963-4149982 MS.gene032215:intron 35.0%
!! GAAACATTTTGGTCATGGTT+TGG + chr2.3:4151686-4151705 None:intergenic 35.0%
!!! CACTTTGAAGCTCAAACTTT+TGG + chr2.3:4149710-4149729 None:intergenic 35.0%
AAAACACCAGTTGACAAGCA+CGG + chr2.3:4149864-4149883 None:intergenic 40.0%
AAATTCTGACCAGCTTCTTC+AGG - chr2.3:4151312-4151331 MS.gene032215:intron 40.0%
AACCAGTAGAAGAACAATGC+TGG + chr2.3:4150560-4150579 None:intergenic 40.0%
AACTCAGTGCTTTACTGTTG+TGG + chr2.3:4149753-4149772 None:intergenic 40.0%
AATTCCCTTGCCATCTGATA+TGG - chr2.3:4151363-4151382 MS.gene032215:intron 40.0%
AATTCTGACCAGCTTCTTCA+GGG - chr2.3:4151313-4151332 MS.gene032215:intron 40.0%
ACATCCCATATTGTTCTCCT+CGG + chr2.3:4150598-4150617 None:intergenic 40.0%
AGAACCGAGGAGAACAATAT+GGG - chr2.3:4150591-4150610 MS.gene032215:intron 40.0%
AGACATCCATACACAGACAA+CGG + chr2.3:4150079-4150098 None:intergenic 40.0%
AGCAGTACCATGCTATTATG+AGG - chr2.3:4151413-4151432 MS.gene032215:intron 40.0%
ATGTCACATACGGATCTCAT+AGG - chr2.3:4151795-4151814 MS.gene032215:CDS 40.0%
CATGCATTAAAGAGAGCTAG+AGG - chr2.3:4151605-4151624 MS.gene032215:intron 40.0%
GATGCTTCTGATGTCACATA+CGG - chr2.3:4151785-4151804 MS.gene032215:CDS 40.0%
GCAAGATATCTCATCGTTGA+TGG - chr2.3:4150363-4150382 MS.gene032215:intron 40.0%
GGAATCCAAAATGCATGATC+AGG - chr2.3:4151745-4151764 MS.gene032215:CDS 40.0%
GTAGAAGAACAATGCTGGAA+AGG + chr2.3:4150555-4150574 None:intergenic 40.0%
TATATAGCCAGTGCTAACCA+AGG + chr2.3:4150180-4150199 None:intergenic 40.0%
TATCAGATGGCAAGGGAATT+CGG + chr2.3:4151363-4151382 None:intergenic 40.0%
TCAATATCAACATGCTGGAG+TGG + chr2.3:4150915-4150934 None:intergenic 40.0%
TCATCTTCAACTCAATCGAC+TGG - chr2.3:4149793-4149812 MS.gene032215:CDS 40.0%
TGAAATGTTTCAGCAGCAAC+AGG - chr2.3:4151820-4151839 MS.gene032215:CDS 40.0%
TGTCGATGTCTAGTCCTTTA+CGG - chr2.3:4151104-4151123 MS.gene032215:intron 40.0%
TTCCAGCATTGTTCTTCTAC+TGG - chr2.3:4150555-4150574 MS.gene032215:intron 40.0%
TTTGCACTGTGTTGGATACT+AGG - chr2.3:4150522-4150541 MS.gene032215:intron 40.0%
! ATGTTTTCGGATGTCCATCA+TGG - chr2.3:4149644-4149663 MS.gene032215:CDS 40.0%
! GAAGCTCAAACTTTTGGACA+TGG + chr2.3:4149704-4149723 None:intergenic 40.0%
! GCACCGACACTTCAAATTAA+AGG - chr2.3:4149954-4149973 MS.gene032215:CDS 40.0%
! GCGAATCAAGCTTTGATTCT+TGG + chr2.3:4151664-4151683 None:intergenic 40.0%
! TCAACATCCCACCATGTTTT+CGG - chr2.3:4149631-4149650 MS.gene032215:CDS 40.0%
! TGACACTTGTTGCTAGTTAC+AGG - chr2.3:4151270-4151289 MS.gene032215:intron 40.0%
! TGTTCTAGTGGATGGAAGTA+TGG - chr2.3:4150714-4150733 MS.gene032215:CDS 40.0%
! TGTTTTCGGATGTCCATCAT+GGG - chr2.3:4149645-4149664 MS.gene032215:CDS 40.0%
!! ATGATGGACATCCGAAAACA+TGG + chr2.3:4149645-4149664 None:intergenic 40.0%
!! GGTTAGCACTGGCTATATAT+TGG - chr2.3:4150181-4150200 MS.gene032215:CDS 40.0%
!! TGGTGGAAACATTTTGGTCA+TGG + chr2.3:4151691-4151710 None:intergenic 40.0%
AAAGTGGAAGCTCTACCACA+AGG - chr2.3:4149724-4149743 MS.gene032215:CDS 45.0%
AGCAACAGGAGTTAGAGTTC+AGG - chr2.3:4151834-4151853 MS.gene032215:CDS 45.0%
AGTTACCCTTCTTCTCAGAC+TGG - chr2.3:4151863-4151882 MS.gene032215:CDS 45.0%
ATACTAATCAAATGGGGGCG+TGG + chr2.3:4150503-4150522 None:intergenic 45.0%
ATCCATTAGTGGAGGATGGA+CGG + chr2.3:4150658-4150677 None:intergenic 45.0%
ATGGACATCCGAAAACATGG+TGG + chr2.3:4149642-4149661 None:intergenic 45.0%
CAACATGCTGGAGTGGAATA+CGG + chr2.3:4150908-4150927 None:intergenic 45.0%
CAATGTCCGTTGTCTGTGTA+TGG - chr2.3:4150070-4150089 MS.gene032215:CDS 45.0%
CAGAAGCAGTTTCCATTAGC+TGG + chr2.3:4151339-4151358 None:intergenic 45.0%
CATACACAGACAACGGACAT+TGG + chr2.3:4150072-4150091 None:intergenic 45.0%
GAGAACCGAGGAGAACAATA+TGG - chr2.3:4150590-4150609 MS.gene032215:intron 45.0%
GGCCATATGAAGCAGAAAGT+AGG + chr2.3:4150822-4150841 None:intergenic 45.0%
GGCCTACTTTCTGCTTCATA+TGG - chr2.3:4150817-4150836 MS.gene032215:intron 45.0%
GTCTTCGCCTCATAATAGCA+TGG + chr2.3:4151423-4151442 None:intergenic 45.0%
TATGCTGATCCAGGCTACAA+TGG - chr2.3:4150796-4150815 MS.gene032215:intron 45.0%
TCAGAAGATCTTCCCCATGA+TGG + chr2.3:4149661-4149680 None:intergenic 45.0%
TCCTTCCAGTCTGAGAAGAA+GGG + chr2.3:4151871-4151890 None:intergenic 45.0%
TGGACATCCGAAAACATGGT+GGG + chr2.3:4149641-4149660 None:intergenic 45.0%
TGTGATCCATTAGTGGAGGA+TGG + chr2.3:4150662-4150681 None:intergenic 45.0%
TTACTGTTGTGGCATCCTTG+TGG + chr2.3:4149742-4149761 None:intergenic 45.0%
TTAGGAGTAGATGTTGCAGC+TGG - chr2.3:4151020-4151039 MS.gene032215:intron 45.0%
TTATGAGGCGAAGACAGTGT+CGG - chr2.3:4151428-4151447 MS.gene032215:intron 45.0%
TTCCTTCCAGTCTGAGAAGA+AGG + chr2.3:4151872-4151891 None:intergenic 45.0%
! GATTGGCTGTGATCCATTAG+TGG + chr2.3:4150669-4150688 None:intergenic 45.0%
! GTTTTCGGATGTCCATCATG+GGG - chr2.3:4149646-4149665 MS.gene032215:CDS 45.0%
! TTAAAGAGAGCTAGAGGTGC+CGG - chr2.3:4151611-4151630 MS.gene032215:intron 45.0%
!!! ATAATTCATTAAATTTTAAA+AGG + chr2.3:4150959-4150978 None:intergenic 5.0%
ACCCTTCTTCTCAGACTGGA+AGG - chr2.3:4151867-4151886 MS.gene032215:CDS 50.0%
AGACACGTGTTAGTGTCCGA+AGG - chr2.3:4149990-4150009 MS.gene032215:intron 50.0%
AGCTGAACTAGTAGTCCCTC+TGG + chr2.3:4151183-4151202 None:intergenic 50.0%
ATAGCCAGTGCTAACCAAGG+AGG + chr2.3:4150177-4150196 None:intergenic 50.0%
CACTAACACGTGTCTCACAC+CGG + chr2.3:4149985-4150004 None:intergenic 50.0%
CTAGACATCGACAGAGAGCA+CGG + chr2.3:4151097-4151116 None:intergenic 50.0%
GAAAGTAGGCCATTGTAGCC+TGG + chr2.3:4150808-4150827 None:intergenic 50.0%
GGTTCTTCCGCCATATCAGA+TGG + chr2.3:4151376-4151395 None:intergenic 50.0%
GTGATTCCGTGCTTGTCAAC+TGG - chr2.3:4149855-4149874 MS.gene032215:CDS 50.0%
TATGAGGCGAAGACAGTGTC+GGG - chr2.3:4151429-4151448 MS.gene032215:intron 50.0%
TCCCTTGCCATCTGATATGG+CGG - chr2.3:4151366-4151385 MS.gene032215:intron 50.0%
TCCGCCATATCAGATGGCAA+GGG + chr2.3:4151370-4151389 None:intergenic 50.0%
TGGCTGTGATCCATTAGTGG+AGG + chr2.3:4150666-4150685 None:intergenic 50.0%
TGTAGCCTGGATCAGCATAG+TGG + chr2.3:4150795-4150814 None:intergenic 50.0%
TGTCGGGACTCGTGAAGATA+TGG + chr2.3:4151581-4151600 None:intergenic 50.0%
TTCCGCCATATCAGATGGCA+AGG + chr2.3:4151371-4151390 None:intergenic 50.0%
! AGCATTGAGAAAACGGCCAC+CGG + chr2.3:4151633-4151652 None:intergenic 50.0%
! CTATGTGAGTCGGTGTCCTT+CGG + chr2.3:4150009-4150028 None:intergenic 50.0%
! TCGACTGGCTCATCACAATC+AGG - chr2.3:4149808-4149827 MS.gene032215:CDS 50.0%
CACCGTCCATCCTCCACTAA+TGG - chr2.3:4150653-4150672 MS.gene032215:intron 55.0%
CCAGTGCTAACCAAGGAGGT+AGG + chr2.3:4150173-4150192 None:intergenic 55.0%
CCTACCTCCTTGGTTAGCAC+TGG - chr2.3:4150170-4150189 MS.gene032215:CDS 55.0%
GCACTCCACTATGCTGATCC+AGG - chr2.3:4150787-4150806 MS.gene032215:intron 55.0%
GCTTCTTCAGGGCCAGCTAA+TGG - chr2.3:4151324-4151343 MS.gene032215:intron 55.0%
GGAGGATGGACGGTGAGATT+TGG + chr2.3:4150648-4150667 None:intergenic 55.0%
TTAGCTGGCCCTGAAGAAGC+TGG + chr2.3:4151324-4151343 None:intergenic 55.0%
!! AAGAGAGCTAGAGGTGCCGG+TGG - chr2.3:4151614-4151633 MS.gene032215:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 4149598 4151958 4149598 ID=MS.gene032215
chr2.3 mRNA 4149598 4151958 4149598 ID=MS.gene032215.t1;Parent=MS.gene032215
chr2.3 exon 4151727 4151958 4151727 ID=MS.gene032215.t1.exon1;Parent=MS.gene032215.t1
chr2.3 CDS 4151727 4151958 4151727 ID=cds.MS.gene032215.t1;Parent=MS.gene032215.t1
chr2.3 exon 4150864 4151012 4150864 ID=MS.gene032215.t1.exon2;Parent=MS.gene032215.t1
chr2.3 CDS 4150864 4151012 4150864 ID=cds.MS.gene032215.t1;Parent=MS.gene032215.t1
chr2.3 exon 4150704 4150778 4150704 ID=MS.gene032215.t1.exon3;Parent=MS.gene032215.t1
chr2.3 CDS 4150704 4150778 4150704 ID=cds.MS.gene032215.t1;Parent=MS.gene032215.t1
chr2.3 exon 4150064 4150264 4150064 ID=MS.gene032215.t1.exon4;Parent=MS.gene032215.t1
chr2.3 CDS 4150064 4150264 4150064 ID=cds.MS.gene032215.t1;Parent=MS.gene032215.t1
chr2.3 exon 4149598 4149978 4149598 ID=MS.gene032215.t1.exon5;Parent=MS.gene032215.t1
chr2.3 CDS 4149598 4149978 4149598 ID=cds.MS.gene032215.t1;Parent=MS.gene032215.t1
Gene Sequence

>MS.gene032215

ATGAAGTGCTTATGTGAGAAAGACTCTGCTCATTCAACATCCCACCATGTTTTCGGATGTCCATCATGGGGAAGATCTTCTGAATCTGAAGTCCAACAAACATCCATGTCCAAAAGTTTGAGCTTCAAAGTGGAAGCTCTACCACAAGGATGCCACAACAGTAAAGCACTGAGTTTTCAATTTCAAGAACAGGATTCATCTTCAACTCAATCGACTGGCTCATCACAATCAGGTATTTGAATTGAAACACTATTTGTGTGATTCCGTGCTTGTCAACTGGTGTTTTCTCATTTTGTTATGTTATTGGTGTATATATCTCCTATTAAGAAACTGCAGTAGTACTTTGTATCTATGTAGCACCGACACTTCAAATTAAAGGTGTCTCCGGTGTGAGACACGTGTTAGTGTCCGAAGGACACCGACTCACATAGTTACATTCGATTACTTCTATTTCTTAAATTATTACTGGTGCCAATGTCCGTTGTCTGTGTATGGATGTCTGTTCTTCATAGCTTTTTCTGGTGATTGAAACAAAATGAAGTGATAGAAGTATATGATTATGATTATGTAAACCTACCTCCTTGGTTAGCACTGGCTATATATTGGTAAAAGCTATGGTCATCCATATAATTTAAGTTTTTCTTATAAAATTGGAATTTGTCTTAGATGGCTCAATCAAAATAACAAAATACCTTGACACGTTACTTCATAACTCATATTGAGAATTCAGCTCCTCATAAGCGAGTCAATTTATAGAATAATAAAGCAAGATATCTCATCGTTGATGGGAAAATAAATGGTTGGTATTCTAACACACCCATTATCTGCTGTGTATGTGTATGTGTATGTAATAACAACAAATTTTAATGTTTGAGATTGCTGAATTACTTAATTTAGTGGAGCCACGCCCCCATTTGATTAGTATTTGCACTGTGTTGGATACTAGGTCACATTCCTTTCCAGCATTGTTCTTCTACTGGTTCAACATTTAAGAGAACCGAGGAGAACAATATGGGATGTCTCATCGAATCATCATCTATTGGGAGTCCAAATCTCACCGTCCATCCTCCACTAATGGATCACAGCCAATCACTTGTAAGCAGATATATATATCTATGTTCTAGTGGATGGAAGTATGGAACATATATGCAGACACTTTTCTCAAAATTTTGGGTTTCAGGCGCATGTTGCACTCCACTATGCTGATCCAGGCTACAATGGCCTACTTTCTGCTTCATATGGCCAACAATATAAGGTAATTTGTATTCAAGATTTTTTATCTCTTTTAATTACGGTTTATTGTGTTACCGTATTCCACTCCAGCATGTTGATATTGATATTAATATTGTATGCAGTTTCCTTTTAAAATTTAATGAATTATGATTATGATCAAATCCTTCAAAAAAAACTTGTAAATAGATGTTAGGAGTAGATGTTGCAGCTGGTACATCTTGTTTATACCTTAATTTGTTCCTCTAAATGGACTGTTCAATCATCCGTGCTCTCTGTCGATGTCTAGTCCTTTACGGCTTTCATAAATCATGATTACATCAAGACTTCTGTTTTTTGCATATATTTGAGACCAGAGGGACTACTAGTTCAGCTTCAATTATTTTGATTTCTTATGAGGTGTATTAGTTCTCTAACTCGAACCATAGTTATGACTGAAAATTGACACTTGTTGCTAGTTACAGGCTTATAGCATCATGTCATCAAATTCTGACCAGCTTCTTCAGGGCCAGCTAATGGAAACTGCTTCTGTCCGAATTCCCTTGCCATCTGATATGGCGGAAGAACCCATATATGTGAATTCAAAGCAGTACCATGCTATTATGAGGCGAAGACAGTGTCGGGCAAAACTTGAAGCACATAACAAACTCATAAAGGATCGTAAAGTAAGCACACATGCATTTCTGTTTTGTCTCTTCTATAGCATAAAATTTCTCTTAAACCAACCGCACTAACAATATTGTTGTGCAGCCATATCTTCACGAGTCCCGACATGTACATGCATTAAAGAGAGCTAGAGGTGCCGGTGGCCGTTTTCTCAATGCTAAAAAGCTCCAAGAATCAAAGCTTGATTCGCCAAACCATGACCAAAATGTTTCCACCAGCTATACTTGTTTGAATTTGAATGGAAATATGTCGGAATCCAAAATGCATGATCAGGTTGAAAACTACAGAGATGATGCTTCTGATGTCACATACGGATCTCATAGGAATGAAATGTTTCAGCAGCAACAGGAGTTAGAGTTCAGGTTATGCAGTTACCCTTCTTCTCAGACTGGAAGGAACATGCATGATTACAAAGCTGACAAGAATGTTGGTGCGAATCAACGCCGACTATCCGTCCGCATGTGA
Protein sequence

>MS.gene032215.t1

MKCLCEKDSAHSTSHHVFGCPSWGRSSESEVQQTSMSKSLSFKVEALPQGCHNSKALSFQFQEQDSSSTQSTGSSQSGHIPFQHCSSTGSTFKRTEENNMGCLIESSSIGSPNLTVHPPLMDHSQSLAHVALHYADPGYNGLLSASYGQQYKAYSIMSSNSDQLLQGQLMETASVRIPLPSDMAEEPIYVNSKQYHAIMRRRQCRAKLEAHNKLIKDRKPYLHESRHVHALKRARGAGGRFLNAKKLQESKLDSPNHDQNVSTSYTCLNLNGNMSESKMHDQVENYRDDASDVTYGSHRNEMFQQQQELEFRLCSYPSSQTGRNMHDYKADKNVGANQRRLSVRM