AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032216

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032216.t1 MTR_2g099500 99.600 250 1 0 1 250 1 250 0.0 514
MS.gene032216.t1 MTR_8g019520 80.081 246 48 1 6 250 10 255 1.42e-123 352
MS.gene032216.t1 MTR_8g019520 79.757 247 48 2 6 250 10 256 5.34e-122 348
MS.gene032216.t1 MTR_2g040990 72.177 248 58 5 6 250 13 252 1.43e-113 326
MS.gene032216.t1 MTR_4g134510 67.857 252 76 1 1 247 1 252 1.74e-111 321
MS.gene032216.t1 MTR_1g015185 68.775 253 70 3 6 250 11 262 2.56e-108 313
MS.gene032216.t1 MTR_4g015830 62.000 250 82 6 1 250 3 239 4.15e-92 271
MS.gene032216.t1 MTR_7g068590 58.607 244 91 4 1 244 3 236 2.92e-86 256
MS.gene032216.t1 MTR_1g015185 79.286 140 29 0 6 145 11 150 1.13e-70 216
MS.gene032216.t1 MTR_8g066480 51.471 136 46 5 116 250 29 145 2.23e-26 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032216.t1 AT3G42790 72.653 245 61 3 6 250 11 249 2.36e-118 338
MS.gene032216.t1 AT5G20510 69.200 250 71 2 6 250 11 259 1.32e-113 327
MS.gene032216.t1 AT1G14510 67.755 245 76 3 6 250 10 251 6.90e-106 306
MS.gene032216.t1 AT2G02470 68.340 259 69 6 1 250 1 255 6.53e-105 304
MS.gene032216.t1 AT2G02470 68.504 254 68 6 1 250 1 246 4.64e-103 299
MS.gene032216.t1 AT5G26210 70.732 246 70 2 6 250 10 254 1.91e-93 275
MS.gene032216.t1 AT5G20510 68.519 216 62 2 40 250 4 218 5.08e-92 270
MS.gene032216.t1 AT5G05610 60.331 242 84 5 6 247 9 238 3.48e-88 261
MS.gene032216.t1 AT5G05610 60.331 242 84 5 6 247 9 238 3.48e-88 261
MS.gene032216.t1 AT3G11200 61.570 242 84 5 6 247 11 243 9.41e-86 255
MS.gene032216.t1 AT5G20510 74.194 155 40 0 6 160 11 165 9.82e-76 228
MS.gene032216.t1 AT3G11200 61.538 208 71 5 40 247 32 230 4.66e-72 220
MS.gene032216.t1 AT2G02470 72.785 158 38 2 1 154 1 157 1.00e-66 207

Find 59 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTCTTCCTCCTCATCTTT+TGG 0.240666 2.3:+4144659 None:intergenic
GGTTGTCTGCTCTGAGCTTC+AGG 0.243347 2.3:+4144698 None:intergenic
TTCTACTTTGGAGCCAGATT+TGG 0.290182 2.3:-4146778 MS.gene032216:CDS
ACGGATTGCTTAATGAGAAT+TGG 0.301755 2.3:-4146945 MS.gene032216:CDS
AAGGAGAATTTGTGCTTATA+CGG 0.310260 2.3:-4146964 MS.gene032216:CDS
AACAGGGCGAAGCCACTTGC+GGG 0.339120 2.3:-4144616 MS.gene032216:CDS
TTTGGCTGTAATGCCGCTTT+TGG 0.349429 2.3:+4144677 None:intergenic
GTAACGGGGCTGATGAGTTC+TGG 0.350703 2.3:-4144580 MS.gene032216:CDS
TGCTGGGTATCAACTTTGCC+AGG 0.351612 2.3:-4146873 MS.gene032216:CDS
CTTTGATGATTCCAGTTCTT+CGG 0.353581 2.3:+4147777 None:intergenic
AAGAAATTATACCGACGGTT+AGG 0.356361 2.3:+4147753 None:intergenic
ATTATTTACCGCTTTAGAAC+TGG 0.360765 2.3:+4145952 None:intergenic
GGGCTTGTGGTGATAGTAAC+GGG 0.362851 2.3:-4144595 MS.gene032216:CDS
AGAGATTCCTGAGCCTGTGC+TGG 0.371017 2.3:-4146890 MS.gene032216:CDS
GATTTCAAGAGCCGAAGAAC+TGG 0.371977 2.3:-4147788 MS.gene032216:CDS
ACAGTATTTGAAGTTGTAAC+TGG 0.378246 2.3:-4146040 MS.gene032216:CDS
CTTTCCCTTGCATTCTACTT+TGG 0.386606 2.3:-4146790 MS.gene032216:CDS
CGGATTGCTTAATGAGAATT+GGG 0.394937 2.3:-4146944 MS.gene032216:CDS
TTTCTCTTGCATTCCATCCC+TGG 0.397443 2.3:+4146855 None:intergenic
AGAGCAGGATGATGATGAAC+AGG 0.397479 2.3:-4144633 MS.gene032216:CDS
AGCTTTATCAAATCCAAATC+TGG 0.410286 2.3:+4146765 None:intergenic
AGCACAGGCTCAGGAATCTC+TGG 0.425752 2.3:+4146892 None:intergenic
AAAAGATGAGGAGGAAGAAC+TGG 0.426868 2.3:-4144657 MS.gene032216:CDS
TCTTGTTACTGCTGCATGAT+GGG 0.433135 2.3:+4144468 None:intergenic
TCTGGAGGAACTTCTTCAAC+AGG 0.445499 2.3:+4146910 None:intergenic
AGAAATTATACCGACGGTTA+GGG 0.452362 2.3:+4147754 None:intergenic
GGAGGAAGAACTGGAAGAGC+AGG 0.470600 2.3:-4144648 MS.gene032216:CDS
CTCTTGTTACTGCTGCATGA+TGG 0.480424 2.3:+4144467 None:intergenic
TTCTACAGACAATGTGATCC+TGG 0.488698 2.3:-4147567 MS.gene032216:intron
TTGATACCCAGCACAGGCTC+AGG 0.497706 2.3:+4146883 None:intergenic
ATCACTGTGAACAGCAACTA+AGG 0.498800 2.3:+4146822 None:intergenic
TTGCTGTTCACAGTGATACA+TGG 0.507689 2.3:-4146816 MS.gene032216:CDS
ATCTGCGAGAAGTGGTTCCA+TGG 0.517485 2.3:-4144545 MS.gene032216:CDS
GGTATCAACTTTGCCAGGGA+TGG 0.520071 2.3:-4146868 MS.gene032216:CDS
GAGATTCCTGAGCCTGTGCT+GGG 0.523852 2.3:-4146889 MS.gene032216:CDS
ATGGAATGCAAGAGAAAGAC+TGG 0.533268 2.3:-4146849 MS.gene032216:CDS
GAAGCCACTTGCGGGGCTTG+TGG 0.543776 2.3:-4144608 MS.gene032216:CDS
TTGATATGCTCAGCACGAGC+AGG 0.552258 2.3:+4144503 None:intergenic
GAATCGAATTGAACAATGGA+AGG 0.552287 2.3:-4147842 None:intergenic
TTGGATTTGATAAAGCTGAC+AGG 0.552742 2.3:-4146759 MS.gene032216:intron
GGGGCTTGTGGTGATAGTAA+CGG 0.558893 2.3:-4144596 MS.gene032216:CDS
GTTACAACTTCAAATACTGT+TGG 0.565683 2.3:+4146043 None:intergenic
GCTGCGACATCTGCGAGAAG+TGG 0.566172 2.3:-4144553 MS.gene032216:CDS
GAACAGGGCGAAGCCACTTG+CGG 0.571946 2.3:-4144617 MS.gene032216:CDS
TGGCTCCAAAGTAGAATGCA+AGG 0.572860 2.3:+4146785 None:intergenic
ATCTAAGTCCAGTTCTAAAG+CGG 0.587642 2.3:-4145960 MS.gene032216:intron
ATCACCACAAGCCCCGCAAG+TGG 0.598002 2.3:+4144604 None:intergenic
TCAGAGCAGACAACCAAAAG+CGG 0.606960 2.3:-4144690 MS.gene032216:CDS
GCTGGGTATCAACTTTGCCA+GGG 0.620748 2.3:-4146872 MS.gene032216:CDS
GCAAAGTTGATACCCAGCAC+AGG 0.629760 2.3:+4146877 None:intergenic
ATCATCAAAGCCCTAACCGT+CGG 0.634254 2.3:-4147764 MS.gene032216:intron
GGCATTACAGCCAAAAGATG+AGG 0.636479 2.3:-4144669 MS.gene032216:CDS
GGCTCCAAAGTAGAATGCAA+GGG 0.639786 2.3:+4146786 None:intergenic
ACAGGGCGAAGCCACTTGCG+GGG 0.643480 2.3:-4144615 MS.gene032216:CDS
ACAGGCTCAGGAATCTCTGG+AGG 0.651339 2.3:+4146895 None:intergenic
ATTACAGCCAAAAGATGAGG+AGG 0.661732 2.3:-4144666 MS.gene032216:CDS
GAGCAGGATGATGATGAACA+GGG 0.682274 2.3:-4144632 MS.gene032216:CDS
GGCTTGTGGTGATAGTAACG+GGG 0.707309 2.3:-4144594 MS.gene032216:CDS
TCTTCAGTCTCAAACAACAG+TGG 0.724521 2.3:-4145989 MS.gene032216:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAGAAAATGATATTATT+AGG + chr2.3:4147178-4147197 None:intergenic 10.0%
!!! AAATAAATACAACTATTTTA+AGG + chr2.3:4145262-4145281 None:intergenic 10.0%
!!! AATAAATACAACTATTTTAA+GGG + chr2.3:4145261-4145280 None:intergenic 10.0%
!! AAATAAATAAGTTAACAATG+TGG - chr2.3:4146838-4146857 MS.gene032216:CDS 15.0%
!! ATTTGTGAATAAAAAAGTAA+AGG - chr2.3:4146711-4146730 MS.gene032216:intron 15.0%
!! GTATATAAACATAACTTATT+TGG + chr2.3:4147446-4147465 None:intergenic 15.0%
!! TAAATAAAATTCAAAAACCT+TGG + chr2.3:4144643-4144662 None:intergenic 15.0%
!!! AAAATGCCTTAATTTTAAAA+AGG + chr2.3:4146660-4146679 None:intergenic 15.0%
!!! AAATGCCTTAATTTTAAAAA+GGG + chr2.3:4146659-4146678 None:intergenic 15.0%
!!! TTCAATCTTTTTCAAAATTT+TGG - chr2.3:4144555-4144574 MS.gene032216:CDS 15.0%
!!! TTTATTGTCATTTTGAATTT+CGG - chr2.3:4147193-4147212 MS.gene032216:intron 15.0%
!!! TTTATTTTCCATCAATTTTA+GGG - chr2.3:4144811-4144830 MS.gene032216:intron 15.0%
!!! TTTTATTTTCCATCAATTTT+AGG - chr2.3:4144810-4144829 MS.gene032216:intron 15.0%
!! AAGAAAAATCTTATTCAACA+AGG + chr2.3:4145699-4145718 None:intergenic 20.0%
!! AAGTATACATATCATTAACA+GGG + chr2.3:4147280-4147299 None:intergenic 20.0%
!! AGAAAAATCTTATTCAACAA+GGG + chr2.3:4145698-4145717 None:intergenic 20.0%
!! AGATAAAAATAGTTATGCTT+CGG + chr2.3:4145561-4145580 None:intergenic 20.0%
!! TATCTAATTGATATCTTGTT+TGG - chr2.3:4147498-4147517 MS.gene032216:intron 20.0%
!! TCTATTGTTTATCCTATAAT+GGG - chr2.3:4146551-4146570 MS.gene032216:intron 20.0%
!! TGATAATTCATTGTTCTTTA+TGG - chr2.3:4144895-4144914 MS.gene032216:intron 20.0%
!! TTAACAATTAGATAAGAACA+CGG + chr2.3:4147548-4147567 None:intergenic 20.0%
!!! AAATTAAGGCATTTTTCAAA+TGG - chr2.3:4146665-4146684 MS.gene032216:intron 20.0%
!!! AGAATCCCTTTTTAAAATTA+AGG - chr2.3:4146651-4146670 MS.gene032216:intron 20.0%
!!! ATAAAAGAGCATTCAAATAT+CGG + chr2.3:4144796-4144815 None:intergenic 20.0%
!!! ATCATCAAAAATTTGTTCAA+CGG + chr2.3:4144479-4144498 None:intergenic 20.0%
!!! ATCTAATTGATATCTTGTTT+GGG - chr2.3:4147499-4147518 MS.gene032216:intron 20.0%
!!! CGCTATTATTATTTTAAACA+GGG + chr2.3:4146471-4146490 None:intergenic 20.0%
!!! TCGCTATTATTATTTTAAAC+AGG + chr2.3:4146472-4146491 None:intergenic 20.0%
!!! TTATTGTCATTTTGAATTTC+GGG - chr2.3:4147194-4147213 MS.gene032216:intron 20.0%
!!! TTTCATTAAATTTTCTACCA+AGG - chr2.3:4144623-4144642 MS.gene032216:CDS 20.0%
!!! TTTTCAAAATTTTGGCAAAA+GGG - chr2.3:4144563-4144582 MS.gene032216:CDS 20.0%
!!! TTTTTCAAAATTTTGGCAAA+AGG - chr2.3:4144562-4144581 MS.gene032216:CDS 20.0%
!!! TTTTTTTGCAATAAACAACA+AGG + chr2.3:4146816-4146835 None:intergenic 20.0%
! AAACATAACTTATTTGGAAG+TGG + chr2.3:4147440-4147459 None:intergenic 25.0%
! ACATTGAAATTAAACTAACC+AGG + chr2.3:4144738-4144757 None:intergenic 25.0%
! ACTTTCAATGCAAAATTATG+TGG - chr2.3:4147464-4147483 MS.gene032216:intron 25.0%
! ATTGACTGATAAATAAAAGG+CGG + chr2.3:4144963-4144982 None:intergenic 25.0%
! CAAGTATACATATCATTAAC+AGG + chr2.3:4147281-4147300 None:intergenic 25.0%
! CTGTTAATGATATGTATACT+TGG - chr2.3:4147279-4147298 MS.gene032216:intron 25.0%
! GAATTTGGACTGAATATTTA+TGG - chr2.3:4147093-4147112 MS.gene032216:intron 25.0%
! GATAAAAATAGTTATGCTTC+GGG + chr2.3:4145560-4145579 None:intergenic 25.0%
! GCAAATAAAATTGCAAATCA+AGG + chr2.3:4145772-4145791 None:intergenic 25.0%
! GTCTATTGTTTATCCTATAA+TGG - chr2.3:4146550-4146569 MS.gene032216:intron 25.0%
! GTGAAGTATGATGAATATAT+TGG - chr2.3:4147029-4147048 MS.gene032216:intron 25.0%
! TAAAGAAAAAACCACTTGAT+GGG + chr2.3:4146062-4146081 None:intergenic 25.0%
! TAATGATATGTATACTTGGT+TGG - chr2.3:4147283-4147302 MS.gene032216:intron 25.0%
! TAATTGAACCCTAAAATTGA+TGG + chr2.3:4144822-4144841 None:intergenic 25.0%
! TATCTCTCATCATTATTAAG+CGG + chr2.3:4147391-4147410 None:intergenic 25.0%
! TATTCTTGCACTAAAATGTT+TGG - chr2.3:4144661-4144680 MS.gene032216:CDS 25.0%
! TTGTCTCCTTCTTTATTAAT+CGG - chr2.3:4146896-4146915 MS.gene032216:CDS 25.0%
!! AAAGGAACAACTAGTTTAAT+TGG - chr2.3:4146729-4146748 MS.gene032216:intron 25.0%
!! AATCATCTGTGTATTTGTTT+TGG - chr2.3:4146116-4146135 MS.gene032216:intron 25.0%
!! ACTAAAGAATCGTTTCATTT+TGG + chr2.3:4146770-4146789 None:intergenic 25.0%
!!! AATAATTTGCTTATCCAAAC+AGG - chr2.3:4145235-4145254 MS.gene032216:intron 25.0%
!!! ATACCATTTTTTCCTGAAAA+AGG - chr2.3:4145857-4145876 MS.gene032216:intron 25.0%
!!! CTTTTATTTATCAGTCAATG+CGG - chr2.3:4144964-4144983 MS.gene032216:intron 25.0%
!!! TAAGCTATTTCTAAGGAAAA+TGG + chr2.3:4145161-4145180 None:intergenic 25.0%
!!! TACCATTTTTTCCTGAAAAA+GGG - chr2.3:4145858-4145877 MS.gene032216:intron 25.0%
AAGGAGAATTTGTGCTTATA+CGG - chr2.3:4145320-4145339 MS.gene032216:intron 30.0%
AAGGTATGCAGAAATAAAAG+AGG - chr2.3:4147140-4147159 MS.gene032216:intron 30.0%
ACTCGTGAAAAAGTTATATC+AGG - chr2.3:4145588-4145607 MS.gene032216:intron 30.0%
AGGGTTCAATTATTTGCAAT+TGG - chr2.3:4144830-4144849 MS.gene032216:intron 30.0%
AGTTTCCACTAACTATGATA+TGG - chr2.3:4147363-4147382 MS.gene032216:intron 30.0%
ATATCAGGATGAGTATGTTA+AGG - chr2.3:4145603-4145622 MS.gene032216:intron 30.0%
CAAAATTTGTAGATCAAGCA+AGG + chr2.3:4144599-4144618 None:intergenic 30.0%
CTAAAGAAAAAACCACTTGA+TGG + chr2.3:4146063-4146082 None:intergenic 30.0%
CTGTTGTATTCTTTCAGAAA+AGG - chr2.3:4145301-4145320 MS.gene032216:intron 30.0%
GTTACAACTTCAAATACTGT+TGG + chr2.3:4146244-4146263 None:intergenic 30.0%
TAAGAACTTTACCTTAAGCT+TGG - chr2.3:4146962-4146981 MS.gene032216:CDS 30.0%
TAGACTCCGATTAATAAAGA+AGG + chr2.3:4146905-4146924 None:intergenic 30.0%
TATACATATCATTAACAGGG+TGG + chr2.3:4147277-4147296 None:intergenic 30.0%
TATGTATACTTGGTTGGAAA+TGG - chr2.3:4147289-4147308 MS.gene032216:intron 30.0%
TCAATGCAAAATTATGTGGA+AGG - chr2.3:4147468-4147487 MS.gene032216:intron 30.0%
TGAACAAGAAATTATACCGA+CGG + chr2.3:4144539-4144558 None:intergenic 30.0%
TTCAAGTGTAGACTGTATAT+AGG - chr2.3:4146627-4146646 MS.gene032216:intron 30.0%
TTGCTTATAAGTCATTGTTG+AGG - chr2.3:4145788-4145807 MS.gene032216:intron 30.0%
TTGTCGCATTTCTATGATTT+AGG - chr2.3:4145009-4145028 MS.gene032216:intron 30.0%
! AATCGGAGTCTAAAATACTT+TGG - chr2.3:4146913-4146932 MS.gene032216:CDS 30.0%
! ACAGTATTTGAAGTTGTAAC+TGG - chr2.3:4146244-4146263 MS.gene032216:intron 30.0%
! AGCTTTATCAAATCCAAATC+TGG + chr2.3:4145522-4145541 None:intergenic 30.0%
! CTTGCTTGATCTACAAATTT+TGG - chr2.3:4144597-4144616 MS.gene032216:CDS 30.0%
! TGCGACAAATTTTGCATTAT+AGG + chr2.3:4144997-4145016 None:intergenic 30.0%
! TGTACTTGTATGATGGAATT+TGG - chr2.3:4147078-4147097 MS.gene032216:intron 30.0%
! TTAATTGGTTTCTTTGAGCA+AGG - chr2.3:4146744-4146763 MS.gene032216:intron 30.0%
!! ATAAGTTTTGTGAGACTGTT+TGG - chr2.3:4145070-4145089 MS.gene032216:intron 30.0%
!! ATTATTTACCGCTTTAGAAC+TGG + chr2.3:4146335-4146354 None:intergenic 30.0%
!! CGCATTGACTGATAAATAAA+AGG + chr2.3:4144966-4144985 None:intergenic 30.0%
!! GCTTACATAAGCTATTTCTA+AGG + chr2.3:4145168-4145187 None:intergenic 30.0%
!! TAAGTTTTGTGAGACTGTTT+GGG - chr2.3:4145071-4145090 MS.gene032216:intron 30.0%
!! TTGGTGTTTCGTTACATTTT+GGG - chr2.3:4146932-4146951 MS.gene032216:CDS 30.0%
!!! ACCATTTTTTCCTGAAAAAG+GGG - chr2.3:4145859-4145878 MS.gene032216:intron 30.0%
!!! TGTTTTAAGATAGTGCAAGA+AGG - chr2.3:4145993-4146012 MS.gene032216:CDS 30.0%
!!! TTTGGTGTTTCGTTACATTT+TGG - chr2.3:4146931-4146950 MS.gene032216:CDS 30.0%
AAGAAATTATACCGACGGTT+AGG + chr2.3:4144534-4144553 None:intergenic 35.0%
AATGGCAAATTAGGACAAGA+AGG + chr2.3:4146180-4146199 None:intergenic 35.0%
AATTGAAGATTCCCATCAAG+TGG - chr2.3:4146048-4146067 MS.gene032216:CDS 35.0%
AGAAATTATACCGACGGTTA+GGG + chr2.3:4144533-4144552 None:intergenic 35.0%
AGTATGTTAAGGCAGTAGTT+TGG - chr2.3:4145614-4145633 MS.gene032216:intron 35.0%
AGTTAGTGGAAACTTGTGAA+GGG + chr2.3:4147357-4147376 None:intergenic 35.0%
ATAGTGCAAGAAGGAAAAGT+TGG - chr2.3:4146002-4146021 MS.gene032216:CDS 35.0%
CAATGCATGAAAGATAACCA+TGG + chr2.3:4146531-4146550 None:intergenic 35.0%
CATGGACAAAAGACAATACA+TGG + chr2.3:4146513-4146532 None:intergenic 35.0%
CGGATTGCTTAATGAGAATT+GGG - chr2.3:4145340-4145359 MS.gene032216:intron 35.0%
GCTAGAAAGCAGTTATAAAG+TGG + chr2.3:4145744-4145763 None:intergenic 35.0%
GCTTATTTGAGCTTATCTAC+TGG - chr2.3:4145046-4145065 MS.gene032216:intron 35.0%
GTCTACACTTGAAAAACCAT+AGG + chr2.3:4146620-4146639 None:intergenic 35.0%
TAGTTAGTGGAAACTTGTGA+AGG + chr2.3:4147358-4147377 None:intergenic 35.0%
TCAAATAAGCCAATCCAAAC+AGG + chr2.3:4145036-4145055 None:intergenic 35.0%
TGAATCGCAGTGTAAAACTT+AGG - chr2.3:4146410-4146429 MS.gene032216:intron 35.0%
TGCAATAAACAACAAGGTCT+TGG + chr2.3:4146810-4146829 None:intergenic 35.0%
TGCATCACAAATGGCAAATT+AGG + chr2.3:4146189-4146208 None:intergenic 35.0%
TTCAAGTGTAGACTGTTCAT+AGG - chr2.3:4146439-4146458 MS.gene032216:intron 35.0%
TTGCAGAATAACCAAGCTTA+AGG + chr2.3:4146976-4146995 None:intergenic 35.0%
TTGGATTTGATAAAGCTGAC+AGG - chr2.3:4145525-4145544 MS.gene032216:intron 35.0%
TTGTTTAGTCATCGTTGTGT+TGG - chr2.3:4145660-4145679 MS.gene032216:intron 35.0%
! ACGGATTGCTTAATGAGAAT+TGG - chr2.3:4145339-4145358 MS.gene032216:intron 35.0%
! CAAGCTTTGTACTTGTATGA+TGG - chr2.3:4147071-4147090 MS.gene032216:intron 35.0%
! CACTTGAATTGCTACTTGTA+AGG - chr2.3:4147121-4147140 MS.gene032216:intron 35.0%
! CATGTATTGTCTTTTGTCCA+TGG - chr2.3:4146511-4146530 MS.gene032216:intron 35.0%
! CTTTGATGATTCCAGTTCTT+CGG + chr2.3:4144510-4144529 None:intergenic 35.0%
! TAATTTGCCATTTGTGATGC+AGG - chr2.3:4146188-4146207 MS.gene032216:intron 35.0%
! TCTATGATTTAGGACCTGTT+TGG - chr2.3:4145019-4145038 MS.gene032216:intron 35.0%
! TGATCTTCACACATTTTCCA+TGG + chr2.3:4147759-4147778 None:intergenic 35.0%
! TGGTGTTTCGTTACATTTTG+GGG - chr2.3:4146933-4146952 MS.gene032216:CDS 35.0%
!! AACTATTTTAAGGGCCTGTT+TGG + chr2.3:4145252-4145271 None:intergenic 35.0%
!! ATCTAAGTCCAGTTCTAAAG+CGG - chr2.3:4146324-4146343 MS.gene032216:intron 35.0%
!! CTTGTAGCTTTGAATTCTGA+TGG - chr2.3:4144923-4144942 MS.gene032216:intron 35.0%
!! TGAATTTCGGGTTTAGAATG+TGG - chr2.3:4147206-4147225 MS.gene032216:intron 35.0%
!!! TCCCCTTTTTCAGGAAAAAA+TGG + chr2.3:4145863-4145882 None:intergenic 35.0%
AAAAGATGAGGAGGAAGAAC+TGG - chr2.3:4147627-4147646 MS.gene032216:intron 40.0%
AAACAACAAGGTCTTGGATG+AGG + chr2.3:4146804-4146823 None:intergenic 40.0%
AGCCACAATTGATGCTCTTT+CGG - chr2.3:4146084-4146103 MS.gene032216:intron 40.0%
ATCACTGTGAACAGCAACTA+AGG + chr2.3:4145465-4145484 None:intergenic 40.0%
ATGGAATGCAAGAGAAAGAC+TGG - chr2.3:4145435-4145454 MS.gene032216:intron 40.0%
ATTACAGCCAAAAGATGAGG+AGG - chr2.3:4147618-4147637 MS.gene032216:intron 40.0%
CAATACATGGAGAGTTTGTC+AGG + chr2.3:4146500-4146519 None:intergenic 40.0%
CACAGACTAAACAATCTGCA+TGG + chr2.3:4146583-4146602 None:intergenic 40.0%
CTTTCCCTTGCATTCTACTT+TGG - chr2.3:4145494-4145513 MS.gene032216:intron 40.0%
TCTTCAGTCTCAAACAACAG+TGG - chr2.3:4146295-4146314 MS.gene032216:intron 40.0%
TCTTGTTACTGCTGCATGAT+GGG + chr2.3:4147819-4147838 None:intergenic 40.0%
TGATGTGACTAAGAGGTCAT+AGG + chr2.3:4145928-4145947 None:intergenic 40.0%
TGTTCTCATCTATCTGCCTA+TGG - chr2.3:4146601-4146620 MS.gene032216:intron 40.0%
TTCTACAGACAATGTGATCC+TGG - chr2.3:4144717-4144736 MS.gene032216:intron 40.0%
! AGTTCTTCCTCCTCATCTTT+TGG + chr2.3:4147628-4147647 None:intergenic 40.0%
! GATTTAGGACCTGTTTGGAT+TGG - chr2.3:4145024-4145043 MS.gene032216:intron 40.0%
! GCACAAGTTTTCAACATGAG+AGG - chr2.3:4147412-4147431 MS.gene032216:intron 40.0%
! TTGCTGTTCACAGTGATACA+TGG - chr2.3:4145468-4145487 MS.gene032216:intron 40.0%
!! GTAGCTTTGAATTCTGATGG+AGG - chr2.3:4144926-4144945 MS.gene032216:intron 40.0%
!! TTCTACTTTGGAGCCAGATT+TGG - chr2.3:4145506-4145525 MS.gene032216:intron 40.0%
ATCATCAAAGCCCTAACCGT+CGG - chr2.3:4144520-4144539 MS.gene032216:CDS 45.0%
CATGGCAGCATTTCTATAGC+AGG - chr2.3:4145950-4145969 MS.gene032216:intron 45.0%
CCATGTGTGATGTGACTAAG+AGG + chr2.3:4145935-4145954 None:intergenic 45.0%
CCTCTTAGTCACATCACACA+TGG - chr2.3:4145932-4145951 MS.gene032216:intron 45.0%
CCTCTTGCAGATGCAAAGTA+AGG + chr2.3:4145887-4145906 None:intergenic 45.0%
CCTTACTTTGCATCTGCAAG+AGG - chr2.3:4145884-4145903 MS.gene032216:intron 45.0%
CTCTTGTTACTGCTGCATGA+TGG + chr2.3:4147820-4147839 None:intergenic 45.0%
GAAGGAGACAAGCTGTAAAC+TGG + chr2.3:4146887-4146906 None:intergenic 45.0%
GACGTTTCCTGCATCACAAA+TGG + chr2.3:4146198-4146217 None:intergenic 45.0%
GATTTCAAGAGCCGAAGAAC+TGG - chr2.3:4144496-4144515 MS.gene032216:CDS 45.0%
GGCATTACAGCCAAAAGATG+AGG - chr2.3:4147615-4147634 MS.gene032216:intron 45.0%
GGCTCCAAAGTAGAATGCAA+GGG + chr2.3:4145501-4145520 None:intergenic 45.0%
GTTGAGGCAGTATTCCAACA+CGG - chr2.3:4145804-4145823 MS.gene032216:intron 45.0%
TCAGAGCAGACAACCAAAAG+CGG - chr2.3:4147594-4147613 MS.gene032216:intron 45.0%
TCTGGAGGAACTTCTTCAAC+AGG + chr2.3:4145377-4145396 None:intergenic 45.0%
TGGAAACTTGTGAAGGGAGA+TGG + chr2.3:4147351-4147370 None:intergenic 45.0%
TGGCTCCAAAGTAGAATGCA+AGG + chr2.3:4145502-4145521 None:intergenic 45.0%
TTTCTCTTGCATTCCATCCC+TGG + chr2.3:4145432-4145451 None:intergenic 45.0%
! AAGTAAGGCTCCCCTTTTTC+AGG + chr2.3:4145872-4145891 None:intergenic 45.0%
! AGAGCAGGATGATGATGAAC+AGG - chr2.3:4147651-4147670 MS.gene032216:intron 45.0%
! GAGCAGGATGATGATGAACA+GGG - chr2.3:4147652-4147671 MS.gene032216:intron 45.0%
!! CGGTGCCATATCATAGTTAG+TGG + chr2.3:4147371-4147390 None:intergenic 45.0%
!! GACCGAAAGAGCATCAATTG+TGG + chr2.3:4146089-4146108 None:intergenic 45.0%
!! GCATGGAGAGTACCCATTAT+AGG + chr2.3:4146566-4146585 None:intergenic 45.0%
!!! TTTGGCTGTAATGCCGCTTT+TGG + chr2.3:4147610-4147629 None:intergenic 45.0%
ATCTGCGAGAAGTGGTTCCA+TGG - chr2.3:4147739-4147758 MS.gene032216:intron 50.0%
GCAGTATTCCAACACGGAGA+CGG - chr2.3:4145810-4145829 MS.gene032216:intron 50.0%
GCTGGGTATCAACTTTGCCA+GGG - chr2.3:4145412-4145431 MS.gene032216:intron 50.0%
GGCTTGTGGTGATAGTAACG+GGG - chr2.3:4147690-4147709 MS.gene032216:intron 50.0%
GGGCTTGTGGTGATAGTAAC+GGG - chr2.3:4147689-4147708 MS.gene032216:intron 50.0%
GGGGCTTGTGGTGATAGTAA+CGG - chr2.3:4147688-4147707 MS.gene032216:intron 50.0%
GGTATCAACTTTGCCAGGGA+TGG - chr2.3:4145416-4145435 MS.gene032216:intron 50.0%
TGCTGGGTATCAACTTTGCC+AGG - chr2.3:4145411-4145430 MS.gene032216:intron 50.0%
TTCAACATCCGTCTCCGTGT+TGG + chr2.3:4145821-4145840 None:intergenic 50.0%
TTGATATGCTCAGCACGAGC+AGG + chr2.3:4147784-4147803 None:intergenic 50.0%
!! GCAAAGTTGATACCCAGCAC+AGG + chr2.3:4145410-4145429 None:intergenic 50.0%
ACAGGCTCAGGAATCTCTGG+AGG + chr2.3:4145392-4145411 None:intergenic 55.0%
AGAGATTCCTGAGCCTGTGC+TGG - chr2.3:4145394-4145413 MS.gene032216:intron 55.0%
AGCACAGGCTCAGGAATCTC+TGG + chr2.3:4145395-4145414 None:intergenic 55.0%
GAGATTCCTGAGCCTGTGCT+GGG - chr2.3:4145395-4145414 MS.gene032216:intron 55.0%
GAGGTCATAGGCTCAAGTCG+TGG + chr2.3:4145916-4145935 None:intergenic 55.0%
GGAGGAAGAACTGGAAGAGC+AGG - chr2.3:4147636-4147655 MS.gene032216:intron 55.0%
TTGATACCCAGCACAGGCTC+AGG + chr2.3:4145404-4145423 None:intergenic 55.0%
! GGTTGTCTGCTCTGAGCTTC+AGG + chr2.3:4147589-4147608 None:intergenic 55.0%
! GTAACGGGGCTGATGAGTTC+TGG - chr2.3:4147704-4147723 MS.gene032216:intron 55.0%
AACAGGGCGAAGCCACTTGC+GGG - chr2.3:4147668-4147687 MS.gene032216:intron 60.0%
ATCACCACAAGCCCCGCAAG+TGG + chr2.3:4147683-4147702 None:intergenic 60.0%
GAACAGGGCGAAGCCACTTG+CGG - chr2.3:4147667-4147686 MS.gene032216:intron 60.0%
GCTGCGACATCTGCGAGAAG+TGG - chr2.3:4147731-4147750 MS.gene032216:intron 60.0%
ACAGGGCGAAGCCACTTGCG+GGG - chr2.3:4147669-4147688 MS.gene032216:intron 65.0%
GAAGCCACTTGCGGGGCTTG+TGG - chr2.3:4147676-4147695 MS.gene032216:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 4144457 4147849 4144457 ID=MS.gene032216
chr2.3 mRNA 4144457 4147849 4144457 ID=MS.gene032216.t1;Parent=MS.gene032216
chr2.3 exon 4147765 4147849 4147765 ID=MS.gene032216.t1.exon1;Parent=MS.gene032216.t1
chr2.3 CDS 4147765 4147849 4147765 ID=cds.MS.gene032216.t1;Parent=MS.gene032216.t1
chr2.3 exon 4147568 4147600 4147568 ID=MS.gene032216.t1.exon2;Parent=MS.gene032216.t1
chr2.3 CDS 4147568 4147600 4147568 ID=cds.MS.gene032216.t1;Parent=MS.gene032216.t1
chr2.3 exon 4146760 4146988 4146760 ID=MS.gene032216.t1.exon3;Parent=MS.gene032216.t1
chr2.3 CDS 4146760 4146988 4146760 ID=cds.MS.gene032216.t1;Parent=MS.gene032216.t1
chr2.3 exon 4145961 4146096 4145961 ID=MS.gene032216.t1.exon4;Parent=MS.gene032216.t1
chr2.3 CDS 4145961 4146096 4145961 ID=cds.MS.gene032216.t1;Parent=MS.gene032216.t1
chr2.3 exon 4144457 4144726 4144457 ID=MS.gene032216.t1.exon5;Parent=MS.gene032216.t1
chr2.3 CDS 4144457 4144726 4144457 ID=cds.MS.gene032216.t1;Parent=MS.gene032216.t1
Gene Sequence

>MS.gene032216

ATGGAAGGTCGAAGTCGCACCGTTGAACAAATTTTTGATGATTTCAAGAGCCGAAGAACTGGAATCATCAAAGCCCTAACCGTCGGTATAATTTCTTGTTCAATCTTTTTCAAAATTTTGGCAAAAGGGTTTTTCATTTCCTTGCTTGATCTACAAATTTTGGATTTTTCATTAAATTTTCTACCAAGGTTTTTGAATTTTATTTATTCTTGCACTAAAATGTTTGGTTTTATTGTCATGTTGTTGCAGATGTTGAAGATTTCTACAGACAATGTGATCCTGGTTAGTTTAATTTCAATGTTTTCTCTCAAAAAAATTATGAATTTCATTTTGTTGCCGATATTTGAATGCTCTTTTATTTTCCATCAATTTTAGGGTTCAATTATTTGCAATTGGTATCACTGATTTGTGTAATTTAGATGTGTTGCGTATGTTAATTGATAATTCATTGTTCTTTATGGCTTTACTTGTAGCTTTGAATTCTGATGGAGGAGATTTTTCAGCCGCCTTTTATTTATCAGTCAATGCGGCTATAAGCCTATAATGCAAAATTTGTCGCATTTCTATGATTTAGGACCTGTTTGGATTGGCTTATTTGAGCTTATCTACTGGCATAAGTTTTGTGAGACTGTTTGGGAGAAGTTTTGAAAACAGCTTATGACATCTTATAGCATATACAAAAACAATTTAACTTTATTTTACCATTTTCCTTAGAAATAGCTTATGTAAGCTTATACATAAGCGCTTATCATGATAAGTGTGTGCGCTATTAGCTGCAAATAATTTGCTTATCCAAACAGGCCCTTAAAATAGTTGTATTTATTTCATATACAAGTTTTCATATCTGTTGTATTCTTTCAGAAAAGGAGAATTTGTGCTTATACGGATTGCTTAATGAGAATTGGGAAGTGAATTTACCTGTTGAAGAAGTTCCTCCAGAGATTCCTGAGCCTGTGCTGGGTATCAACTTTGCCAGGGATGGAATGCAAGAGAAAGACTGGTTAGCCTTAGTTGCTGTTCACAGTGATACATGGTTACTTTCCCTTGCATTCTACTTTGGAGCCAGATTTGGATTTGATAAAGCTGACAGGTATATATTACCCGAAGCATAACTATTTTTATCTGATATTAACTCGTGAAAAAGTTATATCAGGATGAGTATGTTAAGGCAGTAGTTTGGATCATACTTTTCTTATCTTACATTTGTTTAGTCATCGTTGTGTTGGATTTTCGTTACACCCTTGTTGAATAAGATTTTTCTTGTTTAGCATGAAGCAATTGTCTCCACTTTATAACTGCTTTCTAGCATCAGCCTTGATTTGCAATTTTATTTGCTTATAAGTCATTGTTGAGGCAGTATTCCAACACGGAGACGGATGTTGAAGCATAGAAAAAAATGTATACCATTTTTTCCTGAAAAAGGGGAGCCTTACTTTGCATCTGCAAGAGGCTGATTCCACGACTTGAGCCTATGACCTCTTAGTCACATCACACATGGCAGCATTTCTATAGCAGGTTATCTGCTGAGTATCAACTTGTTTTAAGATAGTGCAAGAAGGAAAAGTTGGACTTGTATATTGATTATCATGTGAATTGAAGATTCCCATCAAGTGGTTTTTTCTTTAGCAGCCACAATTGATGCTCTTTCGGTCATAATGTAATCATCTGTGTATTTGTTTTGGATACTTGTTGTTAATGTCACATGCATTTTATCATGCATCCTTCTTGTCCTAATTTGCCATTTGTGATGCAGGAAACGTCTATTCAATTTGATCAATGAACTGCCAACAGTATTTGAAGTTGTAACTGGTGCTGCAAAGAAACAAGTCAAAGAGAAGTCTTCAGTCTCAAACAACAGTGGCAGCAAATCTAAGTCCAGTTCTAAAGCGGTAAATAATTCTTGACAAACATTCATCACAAATAGATACATCTGTTCATAAGTATTTGTCTATCTGAATCGCAGTGTAAAACTTAGGATGTTTTTCAAGTGTAGACTGTTCATAGGTAGAAACCCTGTTTAAAATAATAATAGCGATCTATCCTGACAAACTCTCCATGTATTGTCTTTTGTCCATGGTTATCTTTCATGCATTGTCTATTGTTTATCCTATAATGGGTACTCTCCATGCAGATTGTTTAGTCTGTGTTCTCATCTATCTGCCTATGGTTTTTCAAGTGTAGACTGTATATAGGTAGAATCCCTTTTTAAAATTAAGGCATTTTTCAAATGGTTATTATTATTTGTCTGTTAAGCATTTGTGAATAAAAAAGTAAAGGAACAACTAGTTTAATTGGTTTCTTTGAGCAAGGCCAAAATGAAACGATTCTTTAGTATAAAGTTCTTCCTCATCCAAGACCTTGTTGTTTATTGCAAAAAAAATAAATAAATAAGTTAACAATGTGGTAAACAATCTCTTGATCTTTGAACCAGTTTACAGCTTGTCTCCTTCTTTATTAATCGGAGTCTAAAATACTTTGGTGTTTCGTTACATTTTGGGGATAATGTAAGAACTTTACCTTAAGCTTGGTTATTCTGCAATTGTTTTGACATTGCTTATACATTCAAATGAAAGTGAAGTATGATGAATATATTGGTTGCACTGATTAATTGTTGCAAGCTTTGTACTTGTATGATGGAATTTGGACTGAATATTTATGGCATAACACTTGAATTGCTACTTGTAAGGTATGCAGAAATAAAAGAGGAGAGTAGAACATCCTAATAATATCATTTTCTTTATTGTCATTTTGAATTTCGGGTTTAGAATGTGGATAAGTATAGTATCACATTATTGTTTTCGAAGCATATGATTGAAGCCACCCTGTTAATGATATGTATACTTGGTTGGAAATGGATCATCTCGTGTTGAACTTTCATAATAAAATCGCGTCCATCTCCCTTCACAAGTTTCCACTAACTATGATATGGCACCGCTTAATAATGATGAGAGATATGCACAAGTTTTCAACATGAGAGGATCCACTTCCAAATAAGTTATGTTTATATACTTTCAATGCAAAATTATGTGGAAGGTTAAGAATATCTAATTGATATCTTGTTTGGGTTCTGTTCTAATTCTGAGATAGTCCGTGTTCTTATCTAATTGTTAACTGTTTGAGCAGCGAGCTCCTGAAGCTCAGAGCAGACAACCAAAAGCGGCATTACAGCCAAAAGATGAGGAGGAAGAACTGGAAGAGCAGGATGATGATGAACAGGGCGAAGCCACTTGCGGGGCTTGTGGTGATAGTAACGGGGCTGATGAGTTCTGGATTTGCTGCGACATCTGCGAGAAGTGGTTCCATGGAAAATGTGTGAAGATCACTCCTGCTCGTGCTGAGCATATCAAGCAATACAAGTGCCCATCATGCAGCAGTAACAAGAGAGCTCGCTAG
Protein sequence

>MS.gene032216.t1

MEGRSRTVEQIFDDFKSRRTGIIKALTVDVEDFYRQCDPEKENLCLYGLLNENWEVNLPVEEVPPEIPEPVLGINFARDGMQEKDWLALVAVHSDTWLLSLAFYFGARFGFDKADRKRLFNLINELPTVFEVVTGAAKKQVKEKSSVSNNSGSKSKSSSKARAPEAQSRQPKAALQPKDEEEELEEQDDDEQGEATCGACGDSNGADEFWICCDICEKWFHGKCVKITPARAEHIKQYKCPSCSSNKRAR