AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032455

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032455.t1 MTR_8g051580 93.923 181 11 0 1 181 1 181 1.89e-122 343
MS.gene032455.t1 MTR_1g063160 54.857 175 79 0 1 175 1 175 1.56e-66 201
MS.gene032455.t1 MTR_8g046350 62.130 169 58 2 1 169 1 163 1.58e-66 201
MS.gene032455.t1 MTR_1g047550 54.857 175 79 0 1 175 1 175 6.83e-65 197
MS.gene032455.t1 MTR_8g079502 54.217 166 76 0 1 166 1 166 2.79e-60 186
MS.gene032455.t1 MTR_1g054265 54.167 168 77 0 1 168 1 168 8.98e-60 184
MS.gene032455.t1 MTR_4g131030 49.198 187 78 1 1 170 1 187 1.17e-57 180
MS.gene032455.t1 MTR_4g094632 36.970 165 90 3 1 164 1 152 3.52e-25 95.5
MS.gene032455.t1 MTR_3g080940 37.888 161 73 4 1 138 8 164 8.04e-24 93.6
MS.gene032455.t1 MTR_1g106070 44.554 101 54 1 2 100 9 109 3.67e-22 88.6
MS.gene032455.t1 MTR_3g052870 44.340 106 47 2 2 100 9 109 4.24e-21 86.7
MS.gene032455.t1 MTR_8g086290 40.000 100 58 1 2 101 12 109 5.37e-21 85.5
MS.gene032455.t1 MTR_1g105920 44.340 106 47 2 2 100 9 109 5.55e-21 86.7
MS.gene032455.t1 MTR_5g045560 42.056 107 60 1 1 107 8 112 6.70e-21 86.7
MS.gene032455.t1 MTR_3g052920 44.660 103 45 2 2 97 9 106 1.25e-20 85.9
MS.gene032455.t1 MTR_2g105290 41.414 99 56 1 1 99 11 107 5.16e-20 82.8
MS.gene032455.t1 MTR_3g030770 39.024 123 59 3 2 108 9 131 5.89e-20 84.0
MS.gene032455.t1 MTR_1g105910 43.396 106 48 2 2 100 9 109 7.69e-20 83.6
MS.gene032455.t1 MTR_2g030740 36.538 156 83 4 2 143 9 162 9.67e-20 83.6
MS.gene032455.t1 MTR_1g108580 39.683 126 71 2 2 125 9 131 9.95e-20 83.2
MS.gene032455.t1 MTR_1g108510 39.024 123 59 3 2 108 9 131 2.05e-19 82.4
MS.gene032455.t1 MTR_7g028448 47.297 74 39 0 17 90 27 100 3.07e-19 80.5
MS.gene032455.t1 MTR_4g036915 47.297 74 39 0 17 90 27 100 3.07e-19 80.5
MS.gene032455.t1 MTR_8g043650 42.574 101 56 1 2 100 9 109 7.18e-19 81.3
MS.gene032455.t1 MTR_1g012570 41.509 106 50 2 2 100 9 109 9.41e-19 80.9
MS.gene032455.t1 MTR_1g077360 48.101 79 41 0 2 80 9 87 1.79e-18 80.1
MS.gene032455.t1 MTR_5g053390 48.101 79 41 0 2 80 9 87 2.12e-18 79.7
MS.gene032455.t1 MTR_0121s0100 41.584 101 57 1 2 100 9 109 3.28e-18 77.0
MS.gene032455.t1 MTR_4g094638 40.187 107 61 1 1 104 1 107 4.41e-18 77.8
MS.gene032455.t1 MTR_2g093190 39.683 126 71 2 2 125 9 131 5.44e-18 78.6
MS.gene032455.t1 MTR_1g105905 42.727 110 58 2 18 125 8 114 6.21e-18 78.2
MS.gene032455.t1 MTR_1g075600 53.623 69 32 0 2 70 9 77 6.31e-18 78.6
MS.gene032455.t1 MTR_5g055100 40.351 114 63 1 2 110 9 122 1.30e-17 77.8
MS.gene032455.t1 MTR_2g085280 48.101 79 41 0 2 80 9 87 2.28e-17 77.0
MS.gene032455.t1 MTR_2g085250 48.101 79 41 0 2 80 9 87 2.36e-17 77.0
MS.gene032455.t1 MTR_3g030780 47.826 69 36 0 2 70 9 77 2.54e-17 74.7
MS.gene032455.t1 MTR_0121s0080 61.818 55 21 0 18 72 25 79 3.07e-17 76.6
MS.gene032455.t1 MTR_7g062350 56.667 60 26 0 13 72 32 91 7.41e-17 75.9
MS.gene032455.t1 MTR_4g127140 45.570 79 43 0 1 79 11 89 4.48e-16 72.8
MS.gene032455.t1 MTR_4g093970 37.681 138 70 3 1 130 27 156 1.44e-15 72.4
MS.gene032455.t1 MTR_3g067875 60.417 48 19 0 18 65 8 55 2.42e-15 67.8
MS.gene032455.t1 MTR_2g009890 49.275 69 35 0 1 69 1 69 7.80e-15 70.9
MS.gene032455.t1 MTR_1g108500 55.000 60 27 0 18 77 113 172 7.84e-15 71.2
MS.gene032455.t1 MTR_5g031000 47.826 69 36 0 1 69 1 69 1.07e-14 70.1
MS.gene032455.t1 MTR_5g066180 43.478 69 39 0 1 69 1 69 1.21e-14 69.7
MS.gene032455.t1 MTR_5g031000 47.826 69 36 0 1 69 1 69 1.31e-14 69.3
MS.gene032455.t1 MTR_3g113030 35.252 139 83 3 1 138 1 133 1.58e-14 69.3
MS.gene032455.t1 MTR_1g101970 40.000 75 45 0 1 75 1 75 1.75e-14 68.6
MS.gene032455.t1 MTR_1g053070 43.038 79 45 0 1 79 1 79 1.93e-14 69.3
MS.gene032455.t1 MTR_8g033270 38.947 95 51 1 1 88 1 95 2.06e-14 69.3
MS.gene032455.t1 MTR_0003s0590 47.826 69 36 0 1 69 1 69 2.52e-14 69.3
MS.gene032455.t1 MTR_5g046790 43.662 71 37 1 1 71 1 68 3.01e-14 68.2
MS.gene032455.t1 MTR_5g066960 53.333 60 28 0 1 60 1 60 8.44e-14 63.5
MS.gene032455.t1 MTR_7g075870 43.478 69 39 0 1 69 1 69 8.55e-14 67.0
MS.gene032455.t1 MTR_6g464720 32.353 136 88 3 1 134 1 134 1.07e-13 67.4
MS.gene032455.t1 MTR_8g066260 45.070 71 36 1 1 71 1 68 1.45e-13 67.0
MS.gene032455.t1 MTR_3g452380 46.377 69 37 0 1 69 16 84 1.49e-13 67.0
MS.gene032455.t1 MTR_2g017865 32.143 112 73 1 1 112 16 124 1.71e-13 67.0
MS.gene032455.t1 MTR_8g033220 34.615 104 66 2 1 104 1 102 1.91e-13 66.2
MS.gene032455.t1 MTR_4g109830 45.070 71 36 1 1 71 1 68 2.26e-13 66.2
MS.gene032455.t1 MTR_4g084740 28.205 195 92 5 1 157 1 185 2.31e-13 67.4
MS.gene032455.t1 MTR_8g033220 34.615 104 66 2 1 104 1 102 2.46e-13 66.2
MS.gene032455.t1 MTR_2g461710 49.123 57 29 0 1 57 1 57 2.59e-13 62.0
MS.gene032455.t1 MTR_7g016600 49.180 61 31 0 1 61 1 61 2.84e-13 66.2
MS.gene032455.t1 MTR_3g005530 42.029 69 40 0 1 69 1 69 3.24e-13 65.9
MS.gene032455.t1 MTR_5g021270 45.161 62 34 0 1 62 1 62 5.14e-13 65.1
MS.gene032455.t1 MTR_5g021270 45.161 62 34 0 1 62 1 62 5.24e-13 65.1
MS.gene032455.t1 MTR_4g102530 43.478 69 39 0 1 69 1 69 6.39e-13 64.7
MS.gene032455.t1 MTR_3g067910 49.057 53 27 0 18 70 6 58 9.33e-13 62.0
MS.gene032455.t1 MTR_7g016630 40.000 70 41 1 1 69 1 70 1.19e-12 64.7
MS.gene032455.t1 MTR_7g075870 44.286 70 38 1 1 69 1 70 1.27e-12 63.9
MS.gene032455.t1 MTR_5g046870 49.123 57 29 0 1 57 1 57 1.30e-12 61.6
MS.gene032455.t1 MTR_8g097090 49.180 61 31 0 1 61 1 61 1.32e-12 64.3
MS.gene032455.t1 MTR_8g087860 33.036 112 72 1 1 112 17 125 1.39e-12 64.3
MS.gene032455.t1 MTR_3g084980 50.820 61 30 0 1 61 1 61 1.57e-12 63.9
MS.gene032455.t1 MTR_3g102570 33.333 144 75 6 1 134 1 133 1.81e-12 64.7
MS.gene032455.t1 MTR_6g015975 49.206 63 31 1 1 62 1 63 2.22e-12 63.9
MS.gene032455.t1 MTR_4g093030 38.053 113 64 3 1 111 1 109 2.60e-12 62.0
MS.gene032455.t1 MTR_4g036050 50.909 55 27 0 1 55 1 55 6.58e-12 61.6
MS.gene032455.t1 MTR_4g036050 50.909 55 27 0 1 55 1 55 6.76e-12 61.6
MS.gene032455.t1 MTR_4g036050 50.909 55 27 0 1 55 1 55 7.19e-12 61.6
MS.gene032455.t1 MTR_5g032520 41.538 65 38 0 1 65 1 65 7.32e-12 62.0
MS.gene032455.t1 MTR_4g036050 50.909 55 27 0 1 55 1 55 7.41e-12 61.2
MS.gene032455.t1 MTR_4g109810 45.161 62 34 0 1 62 1 62 7.48e-12 62.4
MS.gene032455.t1 MTR_5g032150 41.538 65 38 0 1 65 1 65 1.00e-11 61.6
MS.gene032455.t1 MTR_7g075850 51.724 58 28 0 1 58 1 58 1.03e-11 62.0
MS.gene032455.t1 MTR_1g041615 64.286 42 15 0 17 58 21 62 2.24e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032455.t1 AT2G34440 39.429 175 90 4 1 162 1 172 1.50e-28 104
MS.gene032455.t1 AT2G24840 43.802 121 66 2 1 120 62 181 2.44e-27 103
MS.gene032455.t1 AT3G66656 44.715 123 66 1 1 121 1 123 5.52e-27 100
MS.gene032455.t1 AT5G60440 44.000 125 53 3 2 117 7 123 1.10e-23 95.1
MS.gene032455.t1 AT1G01530 45.133 113 59 3 1 112 6 116 2.29e-20 85.5
MS.gene032455.t1 AT4G36590 41.732 127 64 4 2 120 7 131 4.13e-20 84.7
MS.gene032455.t1 AT1G47760 52.941 68 32 0 1 68 1 68 2.16e-19 81.3
MS.gene032455.t1 AT1G65360 39.655 116 68 2 1 114 6 121 2.71e-17 76.6
MS.gene032455.t1 AT3G57230 49.275 69 35 0 1 69 1 69 9.72e-17 73.6
MS.gene032455.t1 AT2G22630 50.725 69 34 0 1 69 1 69 1.80e-16 74.7
MS.gene032455.t1 AT2G22630 50.725 69 34 0 1 69 1 69 1.80e-16 74.7
MS.gene032455.t1 AT3G57230 49.275 69 35 0 1 69 1 69 2.59e-16 74.3
MS.gene032455.t1 AT3G57230 49.275 69 35 0 1 69 1 69 2.59e-16 74.3
MS.gene032455.t1 AT1G29962 38.732 142 74 4 2 138 8 141 2.90e-16 73.2
MS.gene032455.t1 AT4G37940 50.725 69 34 0 1 69 1 69 5.47e-16 73.2
MS.gene032455.t1 AT3G61120 42.308 78 45 0 1 78 1 78 7.13e-16 73.2
MS.gene032455.t1 AT4G37940 50.725 69 34 0 1 69 1 69 8.15e-16 72.4
MS.gene032455.t1 AT4G37940 50.725 69 34 0 1 69 1 69 1.40e-15 71.2
MS.gene032455.t1 AT1G28460 44.444 72 40 0 2 73 8 79 2.83e-15 70.5
MS.gene032455.t1 AT3G04100 44.118 68 38 0 2 69 14 81 4.41e-15 70.5
MS.gene032455.t1 AT1G46408 49.367 79 34 3 1 73 1 79 9.67e-15 70.5
MS.gene032455.t1 AT2G45660 42.857 70 40 0 1 70 1 70 1.69e-14 67.8
MS.gene032455.t1 AT2G45650 44.928 69 38 0 1 69 1 69 2.76e-14 68.9
MS.gene032455.t1 AT5G13790 38.889 108 55 2 1 106 1 99 3.35e-14 68.9
MS.gene032455.t1 AT1G69120 47.887 71 34 1 1 71 1 68 3.39e-14 68.6
MS.gene032455.t1 AT4G24540 31.655 139 85 2 1 135 1 133 3.53e-14 68.2
MS.gene032455.t1 AT2G45660 42.857 70 40 0 1 70 1 70 3.77e-14 68.2
MS.gene032455.t1 AT3G57390 46.377 69 37 0 1 69 1 69 4.19e-14 68.2
MS.gene032455.t1 AT4G22950 52.727 55 26 0 8 62 8 62 5.90e-14 66.6
MS.gene032455.t1 AT3G57390 46.377 69 37 0 1 69 1 69 6.12e-14 68.2
MS.gene032455.t1 AT1G69120 47.887 71 34 1 1 71 1 68 6.91e-14 68.2
MS.gene032455.t1 AT5G15800 34.884 172 88 6 1 158 1 162 7.76e-14 67.8
MS.gene032455.t1 AT5G15800 34.884 172 88 6 1 158 1 162 8.23e-14 67.8
MS.gene032455.t1 AT1G71692 41.860 86 45 2 1 85 1 82 8.64e-14 67.0
MS.gene032455.t1 AT1G72350 50.725 69 32 1 2 70 43 109 1.00e-13 67.0
MS.gene032455.t1 AT5G60910 45.070 71 36 1 1 71 1 68 1.15e-13 67.4
MS.gene032455.t1 AT5G13790 38.532 109 57 3 1 106 1 102 1.29e-13 67.4
MS.gene032455.t1 AT2G03710 35.294 170 90 7 1 158 1 162 1.76e-13 65.9
MS.gene032455.t1 AT3G58780 30.000 160 103 5 1 160 16 166 1.81e-13 67.0
MS.gene032455.t1 AT2G22540 46.154 65 35 0 1 65 1 65 2.00e-13 66.6
MS.gene032455.t1 AT2G22540 46.154 65 35 0 1 65 1 65 2.00e-13 66.6
MS.gene032455.t1 AT3G30260 52.632 57 27 0 1 57 1 57 2.03e-13 66.6
MS.gene032455.t1 AT1G17310 57.407 54 21 1 17 70 63 114 2.85e-13 65.9
MS.gene032455.t1 AT3G58780 31.056 161 99 6 1 161 16 164 3.06e-13 66.2
MS.gene032455.t1 AT4G09960 42.029 69 40 0 1 69 2 70 3.23e-13 65.9
MS.gene032455.t1 AT1G22130 47.887 71 36 1 1 70 1 71 3.26e-13 67.0
MS.gene032455.t1 AT4G09960 42.029 69 40 0 1 69 1 69 3.50e-13 65.9
MS.gene032455.t1 AT1G17310 57.407 54 21 1 17 70 74 125 3.52e-13 65.9
MS.gene032455.t1 AT3G58780 31.056 161 99 6 1 161 22 170 3.72e-13 65.9
MS.gene032455.t1 AT2G14210 50.820 61 30 0 1 61 1 61 3.88e-13 65.9
MS.gene032455.t1 AT2G14210 50.820 61 30 0 1 61 1 61 3.91e-13 65.9
MS.gene032455.t1 AT2G03710 35.294 170 90 7 1 158 1 162 4.16e-13 65.9
MS.gene032455.t1 AT4G09960 42.029 69 40 0 1 69 1 69 4.28e-13 65.5
MS.gene032455.t1 AT4G37435 28.090 178 95 4 1 151 1 172 4.38e-13 64.7
MS.gene032455.t1 AT2G03710 35.294 170 90 7 1 158 1 162 4.42e-13 65.9
MS.gene032455.t1 AT4G09960 42.029 69 40 0 1 69 11 79 5.19e-13 65.5
MS.gene032455.t1 AT4G22950 44.928 69 38 0 1 69 1 69 5.25e-13 65.1
MS.gene032455.t1 AT4G22950 44.928 69 38 0 1 69 1 69 5.25e-13 65.1
MS.gene032455.t1 AT4G09960 42.029 69 40 0 1 69 27 95 5.86e-13 65.5
MS.gene032455.t1 AT4G09960 42.029 69 40 0 1 69 96 164 5.93e-13 66.2
MS.gene032455.t1 AT1G26310 46.774 62 33 0 1 62 1 62 6.55e-13 65.5
MS.gene032455.t1 AT3G02310 49.180 61 31 0 1 61 1 61 6.67e-13 64.3
MS.gene032455.t1 AT3G54340 46.774 62 33 0 1 62 1 62 6.73e-13 65.1
MS.gene032455.t1 AT1G77980 43.529 85 43 2 1 84 1 81 7.83e-13 65.9
MS.gene032455.t1 AT5G49490 45.333 75 37 1 3 73 46 120 8.67e-13 65.5
MS.gene032455.t1 AT4G11880 51.852 54 26 0 8 61 8 61 1.26e-12 63.2
MS.gene032455.t1 AT4G11880 51.852 54 26 0 8 61 8 61 1.26e-12 63.2
MS.gene032455.t1 AT2G42830 43.478 69 39 0 1 69 16 84 1.34e-12 64.3
MS.gene032455.t1 AT2G42830 43.478 69 39 0 1 69 16 84 1.43e-12 64.3
MS.gene032455.t1 AT5G65070 33.929 112 70 2 1 110 1 110 2.35e-12 62.0
MS.gene032455.t1 AT5G51860 40.845 71 42 0 1 71 1 71 2.49e-12 63.2
MS.gene032455.t1 AT5G51860 40.845 71 42 0 1 71 1 71 2.58e-12 62.8
MS.gene032455.t1 AT1G24260 49.180 61 31 0 1 61 1 61 2.76e-12 63.5
MS.gene032455.t1 AT5G65070 39.024 82 48 1 1 82 1 80 2.83e-12 62.8
MS.gene032455.t1 AT1G24260 49.180 61 31 0 1 61 1 61 2.90e-12 63.5
MS.gene032455.t1 AT1G24260 49.180 61 31 0 1 61 1 61 3.19e-12 63.2
MS.gene032455.t1 AT3G02310 33.714 175 98 6 1 164 1 168 3.69e-12 63.2
MS.gene032455.t1 AT5G65070 41.026 78 43 2 1 76 1 77 4.17e-12 62.8
MS.gene032455.t1 AT4G11880 49.180 61 31 0 1 61 1 61 4.26e-12 62.8
MS.gene032455.t1 AT4G11880 49.180 61 31 0 1 61 1 61 4.26e-12 62.8
MS.gene032455.t1 AT5G51870 39.130 69 42 0 1 69 1 69 6.21e-12 62.0
MS.gene032455.t1 AT5G10140 49.123 57 29 0 1 57 1 57 6.27e-12 61.2
MS.gene032455.t1 AT5G10140 49.123 57 29 0 1 57 1 57 7.59e-12 61.2
MS.gene032455.t1 AT4G18960 44.118 68 38 0 2 69 18 85 8.20e-12 62.4
MS.gene032455.t1 AT3G58780 37.209 86 41 1 1 73 16 101 8.74e-12 62.0
MS.gene032455.t1 AT5G10140 48.276 58 30 0 1 58 1 58 8.79e-12 61.2
MS.gene032455.t1 AT1G28450 40.278 72 43 0 2 73 8 79 8.83e-12 61.2
MS.gene032455.t1 AT5G51870 31.613 155 84 4 1 146 24 165 9.18e-12 61.2
MS.gene032455.t1 AT5G65070 39.024 82 48 1 1 82 1 80 9.33e-12 62.0
MS.gene032455.t1 AT5G20240 44.444 63 35 0 1 63 1 63 9.65e-12 60.5
MS.gene032455.t1 AT5G10140 49.123 57 29 0 1 57 1 57 1.01e-11 61.2
MS.gene032455.t1 AT5G51870 31.613 155 84 4 1 146 1 142 1.13e-11 60.5
MS.gene032455.t1 AT5G51870 39.130 69 42 0 1 69 1 69 1.18e-11 61.2
MS.gene032455.t1 AT5G49420 44.000 75 38 1 3 73 8 82 1.20e-11 62.4
MS.gene032455.t1 AT1G60920 42.105 76 39 2 3 73 5 80 1.32e-11 60.8
MS.gene032455.t1 AT5G51870 39.130 69 42 0 1 69 11 79 1.54e-11 61.2
MS.gene032455.t1 AT2G22540 47.368 57 30 0 1 57 1 57 1.75e-11 61.2
MS.gene032455.t1 AT5G20240 44.444 63 35 0 1 63 1 63 1.89e-11 60.8
MS.gene032455.t1 AT5G38620 44.595 74 37 1 3 72 6 79 1.93e-11 62.0
MS.gene032455.t1 AT5G65070 36.264 91 56 1 1 91 1 89 2.14e-11 60.5
MS.gene032455.t1 AT5G23260 52.632 57 27 0 1 57 1 57 5.34e-11 60.1
MS.gene032455.t1 AT5G23260 52.632 57 27 0 1 57 17 73 5.38e-11 60.1
MS.gene032455.t1 AT5G23260 52.632 57 27 0 1 57 17 73 5.58e-11 60.1
MS.gene032455.t1 AT1G31140 29.808 104 66 3 2 100 4 105 6.84e-11 59.3
MS.gene032455.t1 AT5G23260 52.632 57 27 0 1 57 1 57 7.02e-11 59.7
MS.gene032455.t1 AT5G04640 32.031 128 74 3 3 117 5 132 7.07e-11 60.1
MS.gene032455.t1 AT5G65080 42.857 70 39 1 1 69 8 77 7.17e-11 58.9
MS.gene032455.t1 AT5G62165 36.232 69 44 0 1 69 1 69 9.47e-11 57.8
MS.gene032455.t1 AT5G62165 36.232 69 44 0 1 69 1 69 9.47e-11 57.8
MS.gene032455.t1 AT5G62165 36.232 69 44 0 1 69 1 69 9.47e-11 57.8
MS.gene032455.t1 AT1G77950 36.471 85 49 2 1 84 1 81 9.60e-11 59.3
MS.gene032455.t1 AT1G77950 36.471 85 49 2 1 84 1 81 9.60e-11 59.3
MS.gene032455.t1 AT1G77950 36.471 85 49 2 1 84 1 81 9.60e-11 59.3
MS.gene032455.t1 AT1G77950 36.471 85 49 2 1 84 1 81 9.60e-11 59.3

Find 43 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCTCTCCTTCAAGAAATT+TGG 0.179978 8.3:-51387342 None:intergenic
CAGTCAGTTTGTATTCTTCT+AGG 0.201316 8.3:-51387493 None:intergenic
GGAATAAGCCTTACTCTTTC+GGG 0.222535 8.3:+51387294 MS.gene032455:CDS
AGATTCATATGGGAAATTTA+AGG 0.261798 8.3:+51387541 MS.gene032455:CDS
GGGAATAAGCCTTACTCTTT+CGG 0.264231 8.3:+51387293 MS.gene032455:CDS
GGCAAATGAACTTGCCATCT+TGG 0.348564 8.3:+51387235 MS.gene032455:CDS
ATATGGGAAATTTAAGGAAA+TGG 0.366214 8.3:+51387547 MS.gene032455:CDS
GCTATTGTTGTGTTCTCCCC+TGG 0.368488 8.3:+51387272 MS.gene032455:CDS
GAGGAAATGAAAGATTCATA+TGG 0.425146 8.3:+51387530 MS.gene032455:CDS
ACATTCTCTAAGCGTCGAAC+AGG 0.429261 8.3:+51387200 MS.gene032455:CDS
CTATTGTTGTGTTCTCCCCT+GGG 0.431123 8.3:+51387273 MS.gene032455:CDS
GCAAATGAACTTGCCATCTT+GGG 0.435150 8.3:+51387236 MS.gene032455:CDS
GTTACTTCTTGCAGAGAAAC+CGG 0.436314 8.3:+51387610 MS.gene032455:CDS
ATGAACTTGCCATCTTGGGC+GGG 0.444356 8.3:+51387240 MS.gene032455:CDS
GTTGCATCCAAATTTCTTGA+AGG 0.449324 8.3:+51387335 MS.gene032455:CDS
AATGTAACCTGCCTTATGAT+AGG 0.459002 8.3:-51387182 None:intergenic
AACAAAGTCTCAAGAAAGAA+AGG 0.467261 8.3:+51387647 MS.gene032455:CDS
AGCTTCTTATGCCATCATCT+AGG 0.477849 8.3:-51387367 None:intergenic
CTTGGGTGCCCGAAAGAGTA+AGG 0.491836 8.3:-51387302 None:intergenic
TAGGATCTCATCATGTGCCC+TGG 0.515236 8.3:-51387474 None:intergenic
AATGAACTTGCCATCTTGGG+CGG 0.518461 8.3:+51387239 MS.gene032455:CDS
TGTGCTAGCTGAACTAAAAG+AGG 0.527386 8.3:+51387439 MS.gene032455:CDS
AAAGAGTAAGGCTTATTCCC+AGG 0.528202 8.3:-51387290 None:intergenic
AGGAAATGAAAGATTCATAT+GGG 0.533599 8.3:+51387531 MS.gene032455:CDS
AAGAGGCTGAAAATGAAGCC+AGG 0.537040 8.3:+51387456 MS.gene032455:CDS
ATGTAACCTGCCTTATGATA+GGG 0.543825 8.3:-51387181 None:intergenic
GTCGCATAATTGCGATTGCG+AGG 0.546324 8.3:+51387150 MS.gene032455:CDS
GGGTGAGAGACCCTATCATA+AGG 0.546640 8.3:+51387171 MS.gene032455:CDS
GATGCAACATCATCAACACT+TGG 0.549337 8.3:-51387320 None:intergenic
TGAACTTGCCATCTTGGGCG+GGG 0.555567 8.3:+51387241 MS.gene032455:CDS
AATTTCTGCCCCGCCCAAGA+TGG 0.555860 8.3:-51387249 None:intergenic
CTTGCAGAGAAACCGGTGGT+TGG 0.563786 8.3:+51387617 MS.gene032455:CDS
GGAGAGCTCAACCTAGATGA+TGG 0.566220 8.3:+51387356 MS.gene032455:CDS
GAGAGACCCTATCATAAGGC+AGG 0.574297 8.3:+51387175 MS.gene032455:CDS
GACTGAACTCACTCAACTTG+AGG 0.582374 8.3:+51387511 MS.gene032455:CDS
AAGAGTAAGGCTTATTCCCA+GGG 0.598555 8.3:-51387289 None:intergenic
AGAGGCTGAAAATGAAGCCA+GGG 0.612352 8.3:+51387457 MS.gene032455:CDS
TCGCATAATTGCGATTGCGA+GGG 0.643845 8.3:+51387151 MS.gene032455:CDS
TCGAACAGGATTATTCAAGA+AGG 0.647981 8.3:+51387214 MS.gene032455:CDS
ATGCAACATCATCAACACTT+GGG 0.663273 8.3:-51387319 None:intergenic
TTCTGAAGTTTCGAACAGAG+AGG 0.689485 8.3:+51387394 MS.gene032455:CDS
ACTTCTTGCAGAGAAACCGG+TGG 0.709944 8.3:+51387613 MS.gene032455:CDS
AGAGTAAGGCTTATTCCCAG+GGG 0.760036 8.3:-51387288 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGATTCATATGGGAAATTTA+AGG + chr8.3:51387541-51387560 MS.gene032455:CDS 25.0%
! AGGAAATGAAAGATTCATAT+GGG + chr8.3:51387531-51387550 MS.gene032455:CDS 25.0%
! ATATGGGAAATTTAAGGAAA+TGG + chr8.3:51387547-51387566 MS.gene032455:CDS 25.0%
AACAAAGTCTCAAGAAAGAA+AGG + chr8.3:51387647-51387666 MS.gene032455:CDS 30.0%
GAGGAAATGAAAGATTCATA+TGG + chr8.3:51387530-51387549 MS.gene032455:CDS 30.0%
AATGTAACCTGCCTTATGAT+AGG - chr8.3:51387185-51387204 None:intergenic 35.0%
ATGCAACATCATCAACACTT+GGG - chr8.3:51387322-51387341 None:intergenic 35.0%
ATGTAACCTGCCTTATGATA+GGG - chr8.3:51387184-51387203 None:intergenic 35.0%
CAGTCAGTTTGTATTCTTCT+AGG - chr8.3:51387496-51387515 None:intergenic 35.0%
GTTGCATCCAAATTTCTTGA+AGG + chr8.3:51387335-51387354 MS.gene032455:CDS 35.0%
TCGAACAGGATTATTCAAGA+AGG + chr8.3:51387214-51387233 MS.gene032455:CDS 35.0%
!!! TTGTTTTTTGTTCCAACCAC+CGG - chr8.3:51387632-51387651 None:intergenic 35.0%
AAAGAGTAAGGCTTATTCCC+AGG - chr8.3:51387293-51387312 None:intergenic 40.0%
AAGAGTAAGGCTTATTCCCA+GGG - chr8.3:51387292-51387311 None:intergenic 40.0%
AGCTTCTTATGCCATCATCT+AGG - chr8.3:51387370-51387389 None:intergenic 40.0%
GAGCTCTCCTTCAAGAAATT+TGG - chr8.3:51387345-51387364 None:intergenic 40.0%
GATGCAACATCATCAACACT+TGG - chr8.3:51387323-51387342 None:intergenic 40.0%
GCAAATGAACTTGCCATCTT+GGG + chr8.3:51387236-51387255 MS.gene032455:CDS 40.0%
GGAATAAGCCTTACTCTTTC+GGG + chr8.3:51387294-51387313 MS.gene032455:CDS 40.0%
GGGAATAAGCCTTACTCTTT+CGG + chr8.3:51387293-51387312 MS.gene032455:CDS 40.0%
GTTACTTCTTGCAGAGAAAC+CGG + chr8.3:51387610-51387629 MS.gene032455:CDS 40.0%
TGTGCTAGCTGAACTAAAAG+AGG + chr8.3:51387439-51387458 MS.gene032455:CDS 40.0%
!! TTCTGAAGTTTCGAACAGAG+AGG + chr8.3:51387394-51387413 MS.gene032455:CDS 40.0%
AAGAGGCTGAAAATGAAGCC+AGG + chr8.3:51387456-51387475 MS.gene032455:CDS 45.0%
AATGAACTTGCCATCTTGGG+CGG + chr8.3:51387239-51387258 MS.gene032455:CDS 45.0%
AGAGGCTGAAAATGAAGCCA+GGG + chr8.3:51387457-51387476 MS.gene032455:CDS 45.0%
AGAGTAAGGCTTATTCCCAG+GGG - chr8.3:51387291-51387310 None:intergenic 45.0%
CTATTGTTGTGTTCTCCCCT+GGG + chr8.3:51387273-51387292 MS.gene032455:CDS 45.0%
GACTGAACTCACTCAACTTG+AGG + chr8.3:51387511-51387530 MS.gene032455:CDS 45.0%
GGCAAATGAACTTGCCATCT+TGG + chr8.3:51387235-51387254 MS.gene032455:CDS 45.0%
TCGCATAATTGCGATTGCGA+GGG + chr8.3:51387151-51387170 MS.gene032455:CDS 45.0%
!! ACATTCTCTAAGCGTCGAAC+AGG + chr8.3:51387200-51387219 MS.gene032455:CDS 45.0%
ACTTCTTGCAGAGAAACCGG+TGG + chr8.3:51387613-51387632 MS.gene032455:CDS 50.0%
ATGAACTTGCCATCTTGGGC+GGG + chr8.3:51387240-51387259 MS.gene032455:CDS 50.0%
GAGAGACCCTATCATAAGGC+AGG + chr8.3:51387175-51387194 MS.gene032455:CDS 50.0%
GCTATTGTTGTGTTCTCCCC+TGG + chr8.3:51387272-51387291 MS.gene032455:CDS 50.0%
GGAGAGCTCAACCTAGATGA+TGG + chr8.3:51387356-51387375 MS.gene032455:CDS 50.0%
GGGTGAGAGACCCTATCATA+AGG + chr8.3:51387171-51387190 MS.gene032455:CDS 50.0%
GTCGCATAATTGCGATTGCG+AGG + chr8.3:51387150-51387169 MS.gene032455:CDS 50.0%
TAGGATCTCATCATGTGCCC+TGG - chr8.3:51387477-51387496 None:intergenic 50.0%
AATTTCTGCCCCGCCCAAGA+TGG - chr8.3:51387252-51387271 None:intergenic 55.0%
CTTGCAGAGAAACCGGTGGT+TGG + chr8.3:51387617-51387636 MS.gene032455:CDS 55.0%
CTTGGGTGCCCGAAAGAGTA+AGG - chr8.3:51387305-51387324 None:intergenic 55.0%
TGAACTTGCCATCTTGGGCG+GGG + chr8.3:51387241-51387260 MS.gene032455:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 51387143 51387688 51387143 ID=MS.gene032455
chr8.3 mRNA 51387143 51387688 51387143 ID=MS.gene032455.t1;Parent=MS.gene032455
chr8.3 exon 51387143 51387688 51387143 ID=MS.gene032455.t1.exon1;Parent=MS.gene032455.t1
chr8.3 CDS 51387143 51387688 51387143 ID=cds.MS.gene032455.t1;Parent=MS.gene032455.t1
Gene Sequence

>MS.gene032455

ATGGGTCGTCGCATAATTGCGATTGCGAGGGTGAGAGACCCTATCATAAGGCAGGTTACATTCTCTAAGCGTCGAACAGGATTATTCAAGAAGGCAAATGAACTTGCCATCTTGGGCGGGGCAGAAATTGCTATTGTTGTGTTCTCCCCTGGGAATAAGCCTTACTCTTTCGGGCACCCAAGTGTTGATGATGTTGCATCCAAATTTCTTGAAGGAGAGCTCAACCTAGATGATGGCATAAGAAGCTCATCTTCTGAAGTTTCGAACAGAGAGGATCTGAATCAACAACTTGAAGATGTGCTAGCTGAACTAAAAGAGGCTGAAAATGAAGCCAGGGCACATGATGAGATCCTAGAAGAATACAAACTGACTGAACTCACTCAACTTGAGGAAATGAAAGATTCATATGGGAAATTTAAGGAAATGGTGAGATCACGTCTTCGTGATTTAGAAACAACTGAATCTATGTTACTTCTTGCAGAGAAACCGGTGGTTGGAACAAAAAACAAAGTCTCAAGAAAGAAAGGAAAACAACCAATGCTTTAG
Protein sequence

>MS.gene032455.t1

MGRRIIAIARVRDPIIRQVTFSKRRTGLFKKANELAILGGAEIAIVVFSPGNKPYSFGHPSVDDVASKFLEGELNLDDGIRSSSSEVSNREDLNQQLEDVLAELKEAENEARAHDEILEEYKLTELTQLEEMKDSYGKFKEMVRSRLRDLETTESMLLLAEKPVVGTKNKVSRKKGKQPML