AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032567

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032567.t1 MTR_7g024760 95.455 176 8 0 1 176 1 176 2.79e-126 352
MS.gene032567.t1 MTR_7g024765 83.815 173 28 0 1 173 1 173 5.98e-107 303
MS.gene032567.t1 MTR_7g024780 67.630 173 55 1 1 172 1 173 1.37e-83 244
MS.gene032567.t1 MTR_7g114410 59.524 168 66 2 7 172 7 174 2.00e-68 206
MS.gene032567.t1 MTR_7g114410 58.553 152 61 2 7 156 7 158 5.26e-59 181
MS.gene032567.t1 MTR_3g463610 51.190 168 77 2 7 169 6 173 7.83e-54 170
MS.gene032567.t1 MTR_1g103110 44.512 164 88 3 7 167 22 185 4.16e-41 137
MS.gene032567.t1 MTR_5g006470 42.771 166 92 2 7 169 20 185 6.58e-39 131
MS.gene032567.t1 MTR_5g006510 42.857 168 90 4 7 169 22 188 3.06e-38 129
MS.gene032567.t1 MTR_5g006460 42.105 171 90 6 7 169 21 190 5.87e-38 129
MS.gene032567.t1 MTR_1g103150 41.566 166 94 3 7 169 22 187 4.05e-37 127
MS.gene032567.t1 MTR_5g006450 41.071 168 94 3 7 169 20 187 1.10e-36 125
MS.gene032567.t1 MTR_5g006520 39.881 168 96 3 7 169 1738 1905 1.37e-31 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032567.t1 AT2G32020 40.462 173 99 3 1 171 12 182 1.35e-44 145
MS.gene032567.t1 AT2G32030 40.964 166 94 3 8 171 24 187 1.07e-42 141
MS.gene032567.t1 AT3G22560 43.976 166 89 3 8 169 7 172 1.18e-42 140

Find 45 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTATTGGGTTCAACTATT+GGG 0.213071 7.2:+74846849 MS.gene032567:CDS
GCAAAGTTTAAAGCAGATTT+AGG 0.222691 7.2:+74846823 MS.gene032567:CDS
ATGCTATTGGGTTCAACTAT+TGG 0.239859 7.2:+74846848 MS.gene032567:CDS
TCCTGATTTGGTAAGGTTGC+AGG 0.261225 7.2:+74846930 MS.gene032567:CDS
GCAGATTTAGGCTATGCTAT+TGG 0.284212 7.2:+74846835 MS.gene032567:CDS
ATTCCAAGACTTTCCTGATT+TGG 0.305046 7.2:+74846918 MS.gene032567:CDS
TGTCTTAATGATCACTCTAT+TGG 0.321892 7.2:+74846772 MS.gene032567:CDS
CAGATTTAGGCTATGCTATT+GGG 0.336174 7.2:+74846836 MS.gene032567:CDS
TTCTGAACAATCCCTCCCTT+TGG 0.370350 7.2:-74847008 None:intergenic
CAAAAGAATCTTCACTGCTT+TGG 0.377958 7.2:-74846887 None:intergenic
TTAAACTTTGCATCAGGATA+AGG 0.382959 7.2:-74846811 None:intergenic
CATTAAGACATATTGATTGT+TGG 0.387585 7.2:-74846759 None:intergenic
GCCTGCAACCTTACCAAATC+AGG 0.389988 7.2:-74846931 None:intergenic
TCAGCATCACTTTCTTTGAA+TGG 0.407648 7.2:-74846622 None:intergenic
AGACATATTGATTGTTGGAA+TGG 0.412290 7.2:-74846754 None:intergenic
GAAAGGGAATTTATAACAAA+GGG 0.412673 7.2:+74847028 MS.gene032567:CDS
TCTGCTTTAAACTTTGCATC+AGG 0.422143 7.2:-74846817 None:intergenic
CTGCTTTGGTGGCTACACCT+TGG 0.450078 7.2:-74846873 None:intergenic
TGCTGATGATGTGTTGTTGT+GGG 0.452299 7.2:+74846639 MS.gene032567:CDS
CATCACTTTCTTTGAATGGC+CGG 0.455673 7.2:-74846618 None:intergenic
AAGACTTTCCTGATTTGGTA+AGG 0.487902 7.2:+74846923 MS.gene032567:CDS
TTACCAAATCAGGAAAGTCT+TGG 0.502664 7.2:-74846921 None:intergenic
CGATGAAATTCCTCATGCTA+AGG 0.513932 7.2:+74847089 MS.gene032567:CDS
GGGTTCAACTATTGGGGCCA+AGG 0.514130 7.2:+74846856 MS.gene032567:CDS
GATTGGAGTTATGTAAATCA+AGG 0.516459 7.2:+74846692 MS.gene032567:CDS
AAGAATCTTCACTGCTTTGG+TGG 0.526653 7.2:-74846884 None:intergenic
AGAAAGGGAATTTATAACAA+AGG 0.541022 7.2:+74847027 MS.gene032567:CDS
ATGCTGATGATGTGTTGTTG+TGG 0.542577 7.2:+74846638 MS.gene032567:CDS
GATGAAATTCCTCATGCTAA+GGG 0.544681 7.2:+74847090 MS.gene032567:CDS
TGGAAAAGGTTGGTTTCCAA+AGG 0.552140 7.2:+74846992 MS.gene032567:CDS
AGGGAGGGATTGTTCAGAAA+GGG 0.555894 7.2:+74847012 MS.gene032567:CDS
AGTCATGCAATATATGCGAT+TGG 0.560410 7.2:+74846675 MS.gene032567:CDS
GGAAAAGGTTGGTTTCCAAA+GGG 0.561564 7.2:+74846993 MS.gene032567:CDS
GCTATTGGGTTCAACTATTG+GGG 0.562007 7.2:+74846850 MS.gene032567:CDS
AAAGGTTGGTTTCCAAAGGG+AGG 0.574627 7.2:+74846996 MS.gene032567:CDS
TAATACTCGTCCAGAGAACA+TGG 0.576553 7.2:+74846954 MS.gene032567:CDS
ATGAATCAAGGATCACTCTC+CGG 0.579292 7.2:+74846599 MS.gene032567:CDS
TGGGAATAGATCATGAATCA+AGG 0.581097 7.2:+74846587 MS.gene032567:CDS
AAGGGAGGGATTGTTCAGAA+AGG 0.588499 7.2:+74847011 MS.gene032567:CDS
ATTGGAGTTATGTAAATCAA+GGG 0.589655 7.2:+74846693 MS.gene032567:CDS
AAGGTTGGTTTCCAAAGGGA+GGG 0.591625 7.2:+74846997 MS.gene032567:CDS
ACTAATTAACCCTTAGCATG+AGG 0.612515 7.2:-74847099 None:intergenic
TTATAACAAAGGGAATGTAG+AGG 0.632962 7.2:+74847038 MS.gene032567:CDS
CTTAGCATGAGGAATTTCAT+CGG 0.640114 7.2:-74847088 None:intergenic
ACTTTGCATCAGGATAAGGA+AGG 0.674910 7.2:-74846807 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAAGGGAATTTATAACAA+AGG + chr7.2:74847027-74847046 MS.gene032567:CDS 25.0%
! ATTGGAGTTATGTAAATCAA+GGG + chr7.2:74846693-74846712 MS.gene032567:CDS 25.0%
! GAAAGGGAATTTATAACAAA+GGG + chr7.2:74847028-74847047 MS.gene032567:CDS 25.0%
!! CATTAAGACATATTGATTGT+TGG - chr7.2:74846762-74846781 None:intergenic 25.0%
GATTGGAGTTATGTAAATCA+AGG + chr7.2:74846692-74846711 MS.gene032567:CDS 30.0%
TGTCTTAATGATCACTCTAT+TGG + chr7.2:74846772-74846791 MS.gene032567:CDS 30.0%
TTAAACTTTGCATCAGGATA+AGG - chr7.2:74846814-74846833 None:intergenic 30.0%
TTATAACAAAGGGAATGTAG+AGG + chr7.2:74847038-74847057 MS.gene032567:CDS 30.0%
TTCTCAAAAACTGTTGGAAA+AGG + chr7.2:74846978-74846997 MS.gene032567:CDS 30.0%
! AGACATATTGATTGTTGGAA+TGG - chr7.2:74846757-74846776 None:intergenic 30.0%
! GCAAAGTTTAAAGCAGATTT+AGG + chr7.2:74846823-74846842 MS.gene032567:CDS 30.0%
AGTCATGCAATATATGCGAT+TGG + chr7.2:74846675-74846694 MS.gene032567:CDS 35.0%
ATGCTATTGGGTTCAACTAT+TGG + chr7.2:74846848-74846867 MS.gene032567:CDS 35.0%
ATTCCAAGACTTTCCTGATT+TGG + chr7.2:74846918-74846937 MS.gene032567:CDS 35.0%
CAAAAACTGTTGGAAAAGGT+TGG + chr7.2:74846982-74847001 MS.gene032567:CDS 35.0%
CAAAAGAATCTTCACTGCTT+TGG - chr7.2:74846890-74846909 None:intergenic 35.0%
CAGATTTAGGCTATGCTATT+GGG + chr7.2:74846836-74846855 MS.gene032567:CDS 35.0%
CTTAGCATGAGGAATTTCAT+CGG - chr7.2:74847091-74847110 None:intergenic 35.0%
GATGAAATTCCTCATGCTAA+GGG + chr7.2:74847090-74847109 MS.gene032567:CDS 35.0%
TCAGCATCACTTTCTTTGAA+TGG - chr7.2:74846625-74846644 None:intergenic 35.0%
TGCTATTGGGTTCAACTATT+GGG + chr7.2:74846849-74846868 MS.gene032567:CDS 35.0%
TGGGAATAGATCATGAATCA+AGG + chr7.2:74846587-74846606 MS.gene032567:CDS 35.0%
TTACCAAATCAGGAAAGTCT+TGG - chr7.2:74846924-74846943 None:intergenic 35.0%
! TCTGCTTTAAACTTTGCATC+AGG - chr7.2:74846820-74846839 None:intergenic 35.0%
!! AAGACTTTCCTGATTTGGTA+AGG + chr7.2:74846923-74846942 MS.gene032567:CDS 35.0%
AAGAATCTTCACTGCTTTGG+TGG - chr7.2:74846887-74846906 None:intergenic 40.0%
ACTTTGCATCAGGATAAGGA+AGG - chr7.2:74846810-74846829 None:intergenic 40.0%
ATGAATCAAGGATCACTCTC+CGG + chr7.2:74846599-74846618 MS.gene032567:CDS 40.0%
CATCACTTTCTTTGAATGGC+CGG - chr7.2:74846621-74846640 None:intergenic 40.0%
CATGGCTTCTCAAAAACTGT+TGG + chr7.2:74846972-74846991 MS.gene032567:CDS 40.0%
CGATGAAATTCCTCATGCTA+AGG + chr7.2:74847089-74847108 MS.gene032567:CDS 40.0%
GCAGATTTAGGCTATGCTAT+TGG + chr7.2:74846835-74846854 MS.gene032567:CDS 40.0%
GCTATTGGGTTCAACTATTG+GGG + chr7.2:74846850-74846869 MS.gene032567:CDS 40.0%
TAATACTCGTCCAGAGAACA+TGG + chr7.2:74846954-74846973 MS.gene032567:CDS 40.0%
! ATGCTGATGATGTGTTGTTG+TGG + chr7.2:74846638-74846657 MS.gene032567:CDS 40.0%
! TGCTGATGATGTGTTGTTGT+GGG + chr7.2:74846639-74846658 MS.gene032567:CDS 40.0%
!! GGAAAAGGTTGGTTTCCAAA+GGG + chr7.2:74846993-74847012 MS.gene032567:CDS 40.0%
!! TGGAAAAGGTTGGTTTCCAA+AGG + chr7.2:74846992-74847011 MS.gene032567:CDS 40.0%
!! TTTTGAGAAGCCATGTTCTC+TGG - chr7.2:74846967-74846986 None:intergenic 40.0%
AAGGGAGGGATTGTTCAGAA+AGG + chr7.2:74847011-74847030 MS.gene032567:CDS 45.0%
AGGGAGGGATTGTTCAGAAA+GGG + chr7.2:74847012-74847031 MS.gene032567:CDS 45.0%
TTCTGAACAATCCCTCCCTT+TGG - chr7.2:74847011-74847030 None:intergenic 45.0%
!! AAAGGTTGGTTTCCAAAGGG+AGG + chr7.2:74846996-74847015 MS.gene032567:CDS 45.0%
!! AAGGTTGGTTTCCAAAGGGA+GGG + chr7.2:74846997-74847016 MS.gene032567:CDS 45.0%
!! TCCTGATTTGGTAAGGTTGC+AGG + chr7.2:74846930-74846949 MS.gene032567:CDS 45.0%
GCCTGCAACCTTACCAAATC+AGG - chr7.2:74846934-74846953 None:intergenic 50.0%
GGGTTCAACTATTGGGGCCA+AGG + chr7.2:74846856-74846875 MS.gene032567:CDS 55.0%
! CTGCTTTGGTGGCTACACCT+TGG - chr7.2:74846876-74846895 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 74846586 74847116 74846586 ID=MS.gene032567
chr7.2 mRNA 74846586 74847116 74846586 ID=MS.gene032567.t1;Parent=MS.gene032567
chr7.2 exon 74846586 74847116 74846586 ID=MS.gene032567.t1.exon1;Parent=MS.gene032567.t1
chr7.2 CDS 74846586 74847116 74846586 ID=cds.MS.gene032567.t1;Parent=MS.gene032567.t1
Gene Sequence

>MS.gene032567

ATGGGAATAGATCATGAATCAAGGATCACTCTCCGGCCATTCAAAGAAAGTGATGCTGATGATGTGTTGTTGTGGGCAAGTGATGAAAGAGTCATGCAATATATGCGATTGGAGTTATGTAAATCAAGGGAACAAGCTTTGAATTTCATAAAAAAAGAGTGCATTTATCCATTCCAACAATCAATATGTCTTAATGATCACTCTATTGGAATTGTTTTTGTCCTTCCTTATCCTGATGCAAAGTTTAAAGCAGATTTAGGCTATGCTATTGGGTTCAACTATTGGGGCCAAGGTGTAGCCACCAAAGCAGTGAAGATTCTTTTGTCTCGTGTATTCCAAGACTTTCCTGATTTGGTAAGGTTGCAGGCTAATACTCGTCCAGAGAACATGGCTTCTCAAAAACTGTTGGAAAAGGTTGGTTTCCAAAGGGAGGGATTGTTCAGAAAGGGAATTTATAACAAAGGGAATGTAGAGGATTTCTGTATTTTTAGTTTCTTGTCTACCGATGAAATTCCTCATGCTAAGGGTTAA
Protein sequence

>MS.gene032567.t1

MGIDHESRITLRPFKESDADDVLLWASDERVMQYMRLELCKSREQALNFIKKECIYPFQQSICLNDHSIGIVFVLPYPDAKFKADLGYAIGFNYWGQGVATKAVKILLSRVFQDFPDLVRLQANTRPENMASQKLLEKVGFQREGLFRKGIYNKGNVEDFCIFSFLSTDEIPHAKG