AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032707

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032707.t1 MTR_5g037080 97.638 254 5 1 1 254 62 314 0.0 505
MS.gene032707.t1 MTR_4g107230 62.963 162 42 6 1 147 31 189 1.49e-45 154
MS.gene032707.t1 MTR_2g100930 64.348 115 32 3 11 121 79 188 1.31e-42 148
MS.gene032707.t1 MTR_4g100630 62.162 111 37 2 21 126 74 184 5.56e-42 146
MS.gene032707.t1 MTR_6g092540 65.169 89 28 1 18 106 77 162 1.14e-37 134
MS.gene032707.t1 MTR_0036s0260 82.353 68 12 0 41 108 28 95 1.20e-35 126
MS.gene032707.t1 MTR_2g090305 80.882 68 13 0 41 108 28 95 7.77e-35 124
MS.gene032707.t1 MTR_3g462790 68.966 87 25 1 30 114 134 220 9.66e-35 127
MS.gene032707.t1 MTR_8g101650 50.833 120 48 3 18 130 85 200 2.51e-34 125
MS.gene032707.t1 MTR_4g111975 72.603 73 20 0 35 107 132 204 6.81e-34 124
MS.gene032707.t1 MTR_5g069710 70.513 78 23 0 35 112 126 203 4.25e-33 122
MS.gene032707.t1 MTR_7g067080 67.949 78 25 0 35 112 134 211 8.69e-32 119
MS.gene032707.t1 MTR_1g111830 68.421 76 24 0 32 107 115 190 1.89e-31 116
MS.gene032707.t1 MTR_5g081860 50.847 118 53 2 28 143 112 226 1.37e-30 115
MS.gene032707.t1 MTR_3g104370 58.442 77 29 1 35 108 85 161 1.24e-25 100
MS.gene032707.t1 MTR_8g063600 60.759 79 29 1 30 106 95 173 1.74e-25 102
MS.gene032707.t1 MTR_4g015130 56.923 65 28 0 51 115 3 67 5.90e-21 84.3
MS.gene032707.t1 MTR_5g088010 40.385 104 56 2 35 132 54 157 1.92e-20 87.4
MS.gene032707.t1 MTR_1g048660 57.812 64 27 0 42 105 13 76 2.58e-20 83.2
MS.gene032707.t1 MTR_5g027570 50.617 81 34 2 34 108 81 161 4.65e-20 86.3
MS.gene032707.t1 MTR_8g063870 56.061 66 27 1 43 106 94 159 1.22e-19 85.9
MS.gene032707.t1 MTR_5g088060 43.750 96 48 2 43 132 274 369 3.96e-19 86.3
MS.gene032707.t1 MTR_5g488160 43.750 96 48 2 43 132 274 369 3.96e-19 86.3
MS.gene032707.t1 MTR_1g033620 72.549 51 14 0 57 107 21 71 6.30e-19 83.6
MS.gene032707.t1 MTR_5g027550 66.667 54 18 0 35 88 71 124 7.83e-19 82.8
MS.gene032707.t1 MTR_5g075760 44.872 78 40 2 32 107 96 172 4.06e-13 67.0
MS.gene032707.t1 MTR_1g083180 48.571 70 32 2 43 109 63 131 7.09e-13 65.9
MS.gene032707.t1 MTR_5g075790 49.254 67 31 2 43 107 115 180 2.07e-12 65.1
MS.gene032707.t1 MTR_3g070110 45.833 72 32 1 43 107 97 168 7.14e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032707.t1 AT1G70000 49.219 256 75 9 1 254 59 261 5.06e-54 175
MS.gene032707.t1 AT1G70000 49.219 256 75 9 1 254 59 261 5.06e-54 175
MS.gene032707.t1 AT5G47390 70.968 93 27 0 18 110 73 165 1.60e-42 148
MS.gene032707.t1 AT1G74840 60.265 151 40 4 1 135 42 188 7.34e-38 133
MS.gene032707.t1 AT1G74840 60.265 151 40 4 1 135 42 188 1.91e-37 132
MS.gene032707.t1 AT1G19000 58.824 153 37 5 1 131 40 188 2.37e-37 133
MS.gene032707.t1 AT1G19000 58.824 153 37 5 1 131 40 188 2.37e-37 133
MS.gene032707.t1 AT3G16350 64.151 106 35 1 19 121 112 217 2.66e-36 132
MS.gene032707.t1 AT5G58900 64.773 88 29 1 31 116 129 216 3.68e-34 124
MS.gene032707.t1 AT5G56840 60.204 98 34 3 20 115 73 167 6.36e-34 122
MS.gene032707.t1 AT1G49010 73.973 73 19 0 35 107 128 200 6.68e-34 124
MS.gene032707.t1 AT5G61620 58.333 96 38 2 19 112 84 179 8.23e-34 124
MS.gene032707.t1 AT2G38090 69.333 75 23 0 36 110 136 210 1.28e-32 121
MS.gene032707.t1 AT5G08520 69.863 73 22 0 35 107 112 184 1.53e-32 120
MS.gene032707.t1 AT5G04760 73.611 72 19 0 36 107 94 165 5.24e-32 117
MS.gene032707.t1 AT5G01200 64.474 76 27 0 35 110 140 215 3.62e-30 114
MS.gene032707.t1 AT3G11280 60.976 82 32 0 30 111 116 197 3.43e-29 111
MS.gene032707.t1 AT3G11280 60.976 82 32 0 30 111 116 197 3.43e-29 111
MS.gene032707.t1 AT5G05790 59.756 82 33 0 30 111 120 201 2.38e-28 109
MS.gene032707.t1 AT5G05790 59.756 82 33 0 30 111 120 201 2.38e-28 109
MS.gene032707.t1 AT5G23650 58.333 72 30 0 35 106 114 185 1.48e-22 95.1
MS.gene032707.t1 AT3G10580 57.143 70 28 1 37 106 91 158 3.05e-22 92.8
MS.gene032707.t1 AT4G09450 51.685 89 39 2 22 106 68 156 3.94e-22 90.9
MS.gene032707.t1 AT3G10580 57.143 70 28 1 37 106 91 158 5.62e-22 92.4
MS.gene032707.t1 AT3G10590 50.000 82 38 2 31 110 99 179 1.77e-19 84.0

Find 88 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATTGGTGTTGGCTTAAT+TGG 0.210658 5.1:-27746306 None:intergenic
ATCTCCGGCGGCGGCGGTTC+TGG 0.294270 5.1:-27745244 None:intergenic
GACGAATGGCTGCTACTTTC+CGG 0.303324 5.1:-27746450 None:intergenic
ATGACCTCGTTTGCGTTCTC+TGG 0.327299 5.1:-27744990 None:intergenic
GCATATCCATCTTCACATTA+TGG 0.334586 5.1:+27746204 MS.gene032707:CDS
GAACACAAGTTGTTCTTGTT+GGG 0.357954 5.1:+27745107 MS.gene032707:CDS
GATGGAACCTTCAACTATAA+TGG 0.360343 5.1:+27746125 MS.gene032707:CDS
CTCATTACCGGCAATGTTAC+CGG 0.380041 5.1:-27746264 None:intergenic
TCACATTATGGCGGCATCCC+TGG 0.390624 5.1:+27746216 MS.gene032707:CDS
GACTGGATCTCCGGCGGCGG+CGG 0.396452 5.1:-27745250 None:intergenic
GTTCTTGTTGGGGTTACAAC+AGG 0.399465 5.1:+27745118 MS.gene032707:CDS
AACAGGTTGGTAAAGGTGAT+TGG 0.399494 5.1:+27745135 MS.gene032707:CDS
ACTCTTTCAGCACTCATTAC+CGG 0.403021 5.1:-27746276 None:intergenic
GTCATTGAAGCTGCAACCTT+CGG 0.407621 5.1:+27746407 MS.gene032707:CDS
TCATCTTCCATTATAGTTGA+AGG 0.430302 5.1:-27746132 None:intergenic
TCTTGGTCCGGTAACATTGC+CGG 0.432456 5.1:+27746257 MS.gene032707:CDS
CACATTATGGCGGCATCCCT+GGG 0.451781 5.1:+27746217 MS.gene032707:CDS
ATGGCTGCAGGGAAGTATAA+TGG 0.461919 5.1:+27746504 MS.gene032707:CDS
AGAACACAAGTTGTTCTTGT+TGG 0.463466 5.1:+27745106 MS.gene032707:CDS
GATACATAACCGGCATCCGC+AGG 0.478265 5.1:-27744938 None:intergenic
CCCATCGGAAAAGGTGTCCC+AGG 0.480895 5.1:-27746234 None:intergenic
TGGTGTTGGCTTAATTGGCT+TGG 0.483452 5.1:-27746301 None:intergenic
GATGTCATCGGATACATAAC+CGG 0.484684 5.1:-27744948 None:intergenic
GGGTTACAACAGGTTGGTAA+AGG 0.495286 5.1:+27745128 MS.gene032707:CDS
AGATTAGCCATCTTAGAAGA+AGG 0.500963 5.1:-27746333 None:intergenic
CTAGAAACTTTGTGAAAACA+AGG 0.504909 5.1:+27745168 MS.gene032707:CDS
GATGACATCGTTCACGCCTC+TGG 0.506341 5.1:+27744962 MS.gene032707:CDS
AGCTTCAATGACAATGGAAA+AGG 0.506440 5.1:-27746396 None:intergenic
TTGTTGATGAAGGTGTTCCT+TGG 0.515417 5.1:+27745075 MS.gene032707:intron
TGTGAAGATGGATATGCGGC+GGG 0.518568 5.1:-27746198 None:intergenic
GGACCAAGACCCATCGGAAA+AGG 0.520312 5.1:-27746243 None:intergenic
GCTGCCTCCTTCTTCTAAGA+TGG 0.520621 5.1:+27746326 MS.gene032707:CDS
TAGCAGCCATTCGTCGTCAT+CGG 0.522877 5.1:+27746458 MS.gene032707:CDS
GGTGGTAATATCAAAGAGAC+TGG 0.526759 5.1:-27745267 None:intergenic
TTAGAAGAAGGAGGCAGCAT+TGG 0.530338 5.1:-27746321 None:intergenic
AACACAAGTTGTTCTTGTTG+GGG 0.535418 5.1:+27745108 MS.gene032707:CDS
GATTCCAACCCTGCGGATGC+CGG 0.536723 5.1:+27744929 MS.gene032707:CDS
GGGTTGGAATCTTGTGGAGG+TGG 0.537810 5.1:-27744917 None:intergenic
GAAGGAGGCAGCATTGGTGT+TGG 0.541098 5.1:-27746315 None:intergenic
AGAGACTGGATCTCCGGCGG+CGG 0.543258 5.1:-27745253 None:intergenic
TTGTGTTCTTCCTCTGTCCA+AGG 0.544021 5.1:-27745092 None:intergenic
ATAACCGGCATCCGCAGGGT+TGG 0.546589 5.1:-27744933 None:intergenic
GCAGGGAAGTATAATGGTGG+GGG 0.547007 5.1:+27746510 MS.gene032707:CDS
ATAACCAGAACCGCCGCCGC+CGG 0.548822 5.1:+27745240 MS.gene032707:CDS
TATCAAAGAGACTGGATCTC+CGG 0.548838 5.1:-27745259 None:intergenic
GTGGTTGTTCATATTGAGAG+AGG 0.549802 5.1:-27744898 None:intergenic
CTTTGATATTACCACCGATA+CGG 0.550867 5.1:+27745274 MS.gene032707:CDS
TTGTTCATATTGAGAGAGGT+TGG 0.557080 5.1:-27744894 None:intergenic
ATGCCGCCATAATGTGAAGA+TGG 0.557994 5.1:-27746210 None:intergenic
GCTGCAACCTTCGGTTCCAT+CGG 0.562274 5.1:+27746416 MS.gene032707:CDS
ATTATACTTCCCTGCAGCCA+TGG 0.565515 5.1:-27746502 None:intergenic
TCCGGTGAATGATCTTCCGA+TGG 0.572768 5.1:-27746432 None:intergenic
AAGAACAAATATTACCGTAT+CGG 0.577972 5.1:-27745288 None:intergenic
CTGCAGGGAAGTATAATGGT+GGG 0.579931 5.1:+27746508 MS.gene032707:CDS
TGCAGGGAAGTATAATGGTG+GGG 0.579949 5.1:+27746509 MS.gene032707:CDS
GGCGGCGGTTCTGGTTATGA+CGG 0.580334 5.1:-27745235 None:intergenic
TCCGCAGGGTTGGAATCTTG+TGG 0.584771 5.1:-27744923 None:intergenic
GCATGACTTGCAACCTGAGT+TGG 0.584887 5.1:-27745194 None:intergenic
TGAAGATGGATATGCGGCGG+GGG 0.585757 5.1:-27746196 None:intergenic
TGACAATGGAAAAGGCTCGA+TGG 0.586187 5.1:-27746388 None:intergenic
TGATCTTCCGATGGAACCGA+AGG 0.587262 5.1:-27746423 None:intergenic
AACAAATATTACCGTATCGG+TGG 0.590836 5.1:-27745285 None:intergenic
GAAAACAAGGACACCAACTC+AGG 0.592034 5.1:+27745181 MS.gene032707:CDS
TTGTTGGGGTTACAACAGGT+TGG 0.610057 5.1:+27745122 MS.gene032707:CDS
GGTTGCAGCTTCAATGACAA+TGG 0.610541 5.1:-27746402 None:intergenic
GCGTTCTCTGGATCGACCAG+AGG 0.617091 5.1:-27744978 None:intergenic
ATGAAGCCGATGACGACGAA+TGG 0.619919 5.1:-27746464 None:intergenic
TGAAGGTGTTCCTTGGACAG+AGG 0.622183 5.1:+27745082 MS.gene032707:intron
GGTTCTGGTTATGACGGCGG+AGG 0.625551 5.1:-27745229 None:intergenic
ATGTGAAGATGGATATGCGG+CGG 0.625832 5.1:-27746199 None:intergenic
ATACATAACCGGCATCCGCA+GGG 0.628032 5.1:-27744937 None:intergenic
AGAGGCGTGAACGATGTCAT+CGG 0.629917 5.1:-27744960 None:intergenic
TCCATCGGAAGATCATTCAC+CGG 0.635009 5.1:+27746431 MS.gene032707:CDS
GTTACCGGACCAAGACCCAT+CGG 0.636658 5.1:-27746249 None:intergenic
CCATCGGAAAAGGTGTCCCA+GGG 0.645708 5.1:-27746233 None:intergenic
GCTGCAGGGAAGTATAATGG+TGG 0.654189 5.1:+27746507 MS.gene032707:CDS
GGTAATGAGTGCTGAAAGAG+TGG 0.656043 5.1:+27746278 MS.gene032707:CDS
GCAGGGTTGGAATCTTGTGG+AGG 0.660646 5.1:-27744920 None:intergenic
CGATCCAGAGAACGCAAACG+AGG 0.663587 5.1:+27744986 MS.gene032707:CDS
GTTGGTAAAGGTGATTGGAG+AGG 0.666734 5.1:+27745140 MS.gene032707:CDS
TTAGCCATCTTAGAAGAAGG+AGG 0.666938 5.1:-27746330 None:intergenic
GGCGGTTCTGGTTATGACGG+CGG 0.676927 5.1:-27745232 None:intergenic
CAAAGAGACTGGATCTCCGG+CGG 0.680570 5.1:-27745256 None:intergenic
TATCCATCTTCACATTATGG+CGG 0.696782 5.1:+27746207 MS.gene032707:CDS
TCAATTTCAGCAAGAAACAG+TGG 0.701868 5.1:+27746155 MS.gene032707:CDS
ATAATGTGAAGATGGATATG+CGG 0.713042 5.1:-27746202 None:intergenic
TCCACAAGATTCCAACCCTG+CGG 0.713162 5.1:+27744922 MS.gene032707:CDS
GTGAAGATGGATATGCGGCG+GGG 0.716036 5.1:-27746197 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATACGAAATATATTTA+AGG - chr5.1:27745414-27745433 None:intergenic 10.0%
!! GAAATTGTTAAAAATATAAA+AGG - chr5.1:27745338-27745357 None:intergenic 10.0%
!! TAAAATCAGATTATTAGAAA+AGG - chr5.1:27745360-27745379 None:intergenic 15.0%
!! TAATATATCATGATTACAAT+TGG - chr5.1:27745667-27745686 None:intergenic 15.0%
! AAGAACAAATATTACCGTAT+CGG - chr5.1:27745291-27745310 None:intergenic 25.0%
! ATGAGCTTATGTTAATTGAT+AGG - chr5.1:27745925-27745944 None:intergenic 25.0%
! CACCTAAAATAAATAATCCA+AGG - chr5.1:27746106-27746125 None:intergenic 25.0%
!! CTATTTCGTTTTACTATAAG+AGG + chr5.1:27745889-27745908 MS.gene032707:intron 25.0%
!! TCATTAATGTAATTTTGCCT+TGG + chr5.1:27746086-27746105 MS.gene032707:intron 25.0%
!!! GGATTATTTATTTTAGGTGA+TGG + chr5.1:27746107-27746126 MS.gene032707:intron 25.0%
!!! GTTTTTTGTTTTGTTGATGA+AGG + chr5.1:27745065-27745084 MS.gene032707:intron 25.0%
!!! TGCCTTGGATTATTTATTTT+AGG + chr5.1:27746101-27746120 MS.gene032707:intron 25.0%
ATAATGTGAAGATGGATATG+CGG - chr5.1:27746205-27746224 None:intergenic 30.0%
ATGTCCGGAGATAATATATT+TGG - chr5.1:27745975-27745994 None:intergenic 30.0%
CTAGAAACTTTGTGAAAACA+AGG + chr5.1:27745168-27745187 MS.gene032707:CDS 30.0%
TCATCTTCCATTATAGTTGA+AGG - chr5.1:27746135-27746154 None:intergenic 30.0%
TGTCCGGAGATAATATATTT+GGG - chr5.1:27745974-27745993 None:intergenic 30.0%
!! CAAACAAAAGTTGATGTATC+TGG - chr5.1:27745024-27745043 None:intergenic 30.0%
AACAAATATTACCGTATCGG+TGG - chr5.1:27745288-27745307 None:intergenic 35.0%
AACACAAGTTGTTCTTGTTG+GGG + chr5.1:27745108-27745127 MS.gene032707:CDS 35.0%
AGAACACAAGTTGTTCTTGT+TGG + chr5.1:27745106-27745125 MS.gene032707:CDS 35.0%
AGCTTCAATGACAATGGAAA+AGG - chr5.1:27746399-27746418 None:intergenic 35.0%
CGGAGATAATATATTTGGGA+CGG - chr5.1:27745970-27745989 None:intergenic 35.0%
CGTCCCAAATATATTATCTC+CGG + chr5.1:27745968-27745987 MS.gene032707:intron 35.0%
CGTTCGCAAAAATATATGTC+CGG - chr5.1:27745990-27746009 None:intergenic 35.0%
CTTTGATATTACCACCGATA+CGG + chr5.1:27745274-27745293 MS.gene032707:CDS 35.0%
GAACACAAGTTGTTCTTGTT+GGG + chr5.1:27745107-27745126 MS.gene032707:CDS 35.0%
GATGGAACCTTCAACTATAA+TGG + chr5.1:27746125-27746144 MS.gene032707:CDS 35.0%
GCATATCCATCTTCACATTA+TGG + chr5.1:27746204-27746223 MS.gene032707:CDS 35.0%
TATCCATCTTCACATTATGG+CGG + chr5.1:27746207-27746226 MS.gene032707:CDS 35.0%
TCAATTTCAGCAAGAAACAG+TGG + chr5.1:27746155-27746174 MS.gene032707:CDS 35.0%
TTGTTCATATTGAGAGAGGT+TGG - chr5.1:27744897-27744916 None:intergenic 35.0%
! AGATAATATATTTGGGACGG+AGG - chr5.1:27745967-27745986 None:intergenic 35.0%
! AGATTAGCCATCTTAGAAGA+AGG - chr5.1:27746336-27746355 None:intergenic 35.0%
! GATAATATATTTGGGACGGA+GGG - chr5.1:27745966-27745985 None:intergenic 35.0%
!!! ATCATCGCCTTTTAAAACCA+TGG + chr5.1:27746485-27746504 MS.gene032707:CDS 35.0%
ACTCTTTCAGCACTCATTAC+CGG - chr5.1:27746279-27746298 None:intergenic 40.0%
GATGTCATCGGATACATAAC+CGG - chr5.1:27744951-27744970 None:intergenic 40.0%
GGTGGTAATATCAAAGAGAC+TGG - chr5.1:27745270-27745289 None:intergenic 40.0%
GTGGTTGTTCATATTGAGAG+AGG - chr5.1:27744901-27744920 None:intergenic 40.0%
TATCAAAGAGACTGGATCTC+CGG - chr5.1:27745262-27745281 None:intergenic 40.0%
TTAGCCATCTTAGAAGAAGG+AGG - chr5.1:27746333-27746352 None:intergenic 40.0%
!! AACAGGTTGGTAAAGGTGAT+TGG + chr5.1:27745135-27745154 MS.gene032707:CDS 40.0%
!! AGCATTGGTGTTGGCTTAAT+TGG - chr5.1:27746309-27746328 None:intergenic 40.0%
!! TTGTTGATGAAGGTGTTCCT+TGG + chr5.1:27745075-27745094 MS.gene032707:intron 40.0%
!!! CTTTTAAAACCATGGCTGCA+GGG + chr5.1:27746493-27746512 MS.gene032707:CDS 40.0%
ATGCCGCCATAATGTGAAGA+TGG - chr5.1:27746213-27746232 None:intergenic 45.0%
ATGTGAAGATGGATATGCGG+CGG - chr5.1:27746202-27746221 None:intergenic 45.0%
ATTATACTTCCCTGCAGCCA+TGG - chr5.1:27746505-27746524 None:intergenic 45.0%
CTCATTACCGGCAATGTTAC+CGG - chr5.1:27746267-27746286 None:intergenic 45.0%
GAAAACAAGGACACCAACTC+AGG + chr5.1:27745181-27745200 MS.gene032707:CDS 45.0%
GGTTGCAGCTTCAATGACAA+TGG - chr5.1:27746405-27746424 None:intergenic 45.0%
GTCATTGAAGCTGCAACCTT+CGG + chr5.1:27746407-27746426 MS.gene032707:CDS 45.0%
GTTCTTGTTGGGGTTACAAC+AGG + chr5.1:27745118-27745137 MS.gene032707:CDS 45.0%
TCCATCGGAAGATCATTCAC+CGG + chr5.1:27746431-27746450 MS.gene032707:CDS 45.0%
TGACAATGGAAAAGGCTCGA+TGG - chr5.1:27746391-27746410 None:intergenic 45.0%
TTAGAAGAAGGAGGCAGCAT+TGG - chr5.1:27746324-27746343 None:intergenic 45.0%
TTGTGTTCTTCCTCTGTCCA+AGG - chr5.1:27745095-27745114 None:intergenic 45.0%
TTGTTGGGGTTACAACAGGT+TGG + chr5.1:27745122-27745141 MS.gene032707:CDS 45.0%
! ATGGCTGCAGGGAAGTATAA+TGG + chr5.1:27746504-27746523 MS.gene032707:CDS 45.0%
! CTGCAGGGAAGTATAATGGT+GGG + chr5.1:27746508-27746527 MS.gene032707:CDS 45.0%
! GTTGGTAAAGGTGATTGGAG+AGG + chr5.1:27745140-27745159 MS.gene032707:CDS 45.0%
! TGCAGGGAAGTATAATGGTG+GGG + chr5.1:27746509-27746528 MS.gene032707:CDS 45.0%
!! GGGTTACAACAGGTTGGTAA+AGG + chr5.1:27745128-27745147 MS.gene032707:CDS 45.0%
!! GGTAATGAGTGCTGAAAGAG+TGG + chr5.1:27746278-27746297 MS.gene032707:CDS 45.0%
!! TGGTGTTGGCTTAATTGGCT+TGG - chr5.1:27746304-27746323 None:intergenic 45.0%
!!! CCTGCAGCCATGGTTTTAAA+AGG - chr5.1:27746495-27746514 None:intergenic 45.0%
!!! CCTTTTAAAACCATGGCTGC+AGG + chr5.1:27746492-27746511 MS.gene032707:CDS 45.0%
ATACATAACCGGCATCCGCA+GGG - chr5.1:27744940-27744959 None:intergenic 50.0%
ATGAAGCCGATGACGACGAA+TGG - chr5.1:27746467-27746486 None:intergenic 50.0%
ATGACCTCGTTTGCGTTCTC+TGG - chr5.1:27744993-27745012 None:intergenic 50.0%
TAGCAGCCATTCGTCGTCAT+CGG + chr5.1:27746458-27746477 MS.gene032707:CDS 50.0%
TCCACAAGATTCCAACCCTG+CGG + chr5.1:27744922-27744941 MS.gene032707:CDS 50.0%
TCCGGTGAATGATCTTCCGA+TGG - chr5.1:27746435-27746454 None:intergenic 50.0%
TCTTGGTCCGGTAACATTGC+CGG + chr5.1:27746257-27746276 MS.gene032707:CDS 50.0%
TGATCTTCCGATGGAACCGA+AGG - chr5.1:27746426-27746445 None:intergenic 50.0%
TGTGAAGATGGATATGCGGC+GGG - chr5.1:27746201-27746220 None:intergenic 50.0%
! ACACCTTTTCCGATGGGTCT+TGG + chr5.1:27746240-27746259 MS.gene032707:CDS 50.0%
! AGAGGCGTGAACGATGTCAT+CGG - chr5.1:27744963-27744982 None:intergenic 50.0%
! GAAGTATAATGGTGGGGGTG+GGG + chr5.1:27746515-27746534 MS.gene032707:CDS 50.0%
! GCAGGGAAGTATAATGGTGG+GGG + chr5.1:27746510-27746529 MS.gene032707:CDS 50.0%
! GCATGACTTGCAACCTGAGT+TGG - chr5.1:27745197-27745216 None:intergenic 50.0%
! GCTGCAGGGAAGTATAATGG+TGG + chr5.1:27746507-27746526 MS.gene032707:CDS 50.0%
! GCTGCCTCCTTCTTCTAAGA+TGG + chr5.1:27746326-27746345 MS.gene032707:CDS 50.0%
! GGAAGTATAATGGTGGGGGT+GGG + chr5.1:27746514-27746533 MS.gene032707:CDS 50.0%
! TTTTCCGATGGGTCTTGGTC+CGG + chr5.1:27746245-27746264 MS.gene032707:CDS 50.0%
!! GACGAATGGCTGCTACTTTC+CGG - chr5.1:27746453-27746472 None:intergenic 50.0%
!! TGAAGGTGTTCCTTGGACAG+AGG + chr5.1:27745082-27745101 MS.gene032707:intron 50.0%
CAAAGAGACTGGATCTCCGG+CGG - chr5.1:27745259-27745278 None:intergenic 55.0%
CACATTATGGCGGCATCCCT+GGG + chr5.1:27746217-27746236 MS.gene032707:CDS 55.0%
CGATCCAGAGAACGCAAACG+AGG + chr5.1:27744986-27745005 MS.gene032707:CDS 55.0%
GATACATAACCGGCATCCGC+AGG - chr5.1:27744941-27744960 None:intergenic 55.0%
GATGACATCGTTCACGCCTC+TGG + chr5.1:27744962-27744981 MS.gene032707:CDS 55.0%
GCAGGGTTGGAATCTTGTGG+AGG - chr5.1:27744923-27744942 None:intergenic 55.0%
GCTGCAACCTTCGGTTCCAT+CGG + chr5.1:27746416-27746435 MS.gene032707:CDS 55.0%
GGACCAAGACCCATCGGAAA+AGG - chr5.1:27746246-27746265 None:intergenic 55.0%
GGGTTGGAATCTTGTGGAGG+TGG - chr5.1:27744920-27744939 None:intergenic 55.0%
GTGAAGATGGATATGCGGCG+GGG - chr5.1:27746200-27746219 None:intergenic 55.0%
GTTACCGGACCAAGACCCAT+CGG - chr5.1:27746252-27746271 None:intergenic 55.0%
TCACATTATGGCGGCATCCC+TGG + chr5.1:27746216-27746235 MS.gene032707:CDS 55.0%
TCCGCAGGGTTGGAATCTTG+TGG - chr5.1:27744926-27744945 None:intergenic 55.0%
TGAAGATGGATATGCGGCGG+GGG - chr5.1:27746199-27746218 None:intergenic 55.0%
! CCTGGGACACCTTTTCCGAT+GGG + chr5.1:27746234-27746253 MS.gene032707:CDS 55.0%
! GGGAAGTATAATGGTGGGGG+TGG + chr5.1:27746513-27746532 MS.gene032707:CDS 55.0%
!! CCATCGGAAAAGGTGTCCCA+GGG - chr5.1:27746236-27746255 None:intergenic 55.0%
!! GAAGGAGGCAGCATTGGTGT+TGG - chr5.1:27746318-27746337 None:intergenic 55.0%
ATAACCGGCATCCGCAGGGT+TGG - chr5.1:27744936-27744955 None:intergenic 60.0%
GATTCCAACCCTGCGGATGC+CGG + chr5.1:27744929-27744948 MS.gene032707:CDS 60.0%
GCGTTCTCTGGATCGACCAG+AGG - chr5.1:27744981-27745000 None:intergenic 60.0%
! CCCTGGGACACCTTTTCCGA+TGG + chr5.1:27746233-27746252 MS.gene032707:CDS 60.0%
! GGCGGCGGTTCTGGTTATGA+CGG - chr5.1:27745238-27745257 None:intergenic 60.0%
! GGCGGTTCTGGTTATGACGG+CGG - chr5.1:27745235-27745254 None:intergenic 60.0%
! GGTTCTGGTTATGACGGCGG+AGG - chr5.1:27745232-27745251 None:intergenic 60.0%
!! CCCATCGGAAAAGGTGTCCC+AGG - chr5.1:27746237-27746256 None:intergenic 60.0%
AGAGACTGGATCTCCGGCGG+CGG - chr5.1:27745256-27745275 None:intergenic 65.0%
ATAACCAGAACCGCCGCCGC+CGG + chr5.1:27745240-27745259 MS.gene032707:CDS 65.0%
GATGGATATGCGGCGGGGGT+TGG - chr5.1:27746195-27746214 None:intergenic 65.0%
GGATATGCGGCGGGGGTTGG+TGG - chr5.1:27746192-27746211 None:intergenic 70.0%
!! TATGCGGCGGGGGTTGGTGG+TGG - chr5.1:27746189-27746208 None:intergenic 70.0%
ATCTCCGGCGGCGGCGGTTC+TGG - chr5.1:27745247-27745266 None:intergenic 75.0%
GACTGGATCTCCGGCGGCGG+CGG - chr5.1:27745253-27745272 None:intergenic 75.0%
!! GCGGGGGTTGGTGGTGGCAG+TGG - chr5.1:27746183-27746202 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 27744890 27746560 27744890 ID=MS.gene032707
chr5.1 mRNA 27744890 27746560 27744890 ID=MS.gene032707.t1;Parent=MS.gene032707
chr5.1 exon 27744890 27745007 27744890 ID=MS.gene032707.t1.exon1;Parent=MS.gene032707.t1
chr5.1 CDS 27744890 27745007 27744890 ID=cds.MS.gene032707.t1;Parent=MS.gene032707.t1
chr5.1 exon 27745087 27745295 27745087 ID=MS.gene032707.t1.exon2;Parent=MS.gene032707.t1
chr5.1 CDS 27745087 27745295 27745087 ID=cds.MS.gene032707.t1;Parent=MS.gene032707.t1
chr5.1 exon 27746123 27746560 27746123 ID=MS.gene032707.t1.exon3;Parent=MS.gene032707.t1
chr5.1 CDS 27746123 27746560 27746123 ID=cds.MS.gene032707.t1;Parent=MS.gene032707.t1
Gene Sequence

>MS.gene032707

ATGACCAACCTCTCTCAATATGAACAACCACCTCCACAAGATTCCAACCCTGCGGATGCCGGTTATGTATCCGATGACATCGTTCACGCCTCTGGTCGATCCAGAGAACGCAAACGAGGTCATTATTTAAACCAGATACATCAACTTTTGTTTGATATTGCTTATTTATATTAATGTTTTTTGTTTTGTTGATGAAGGTGTTCCTTGGACAGAGGAAGAACACAAGTTGTTCTTGTTGGGGTTACAACAGGTTGGTAAAGGTGATTGGAGAGGAATTTCTAGAAACTTTGTGAAAACAAGGACACCAACTCAGGTTGCAAGTCATGCTCAGAAGTATTTCCTCCGCCGTCATAACCAGAACCGCCGCCGCCGGAGATCCAGTCTCTTTGATATTACCACCGATACGGTAATATTTGTTCTTTGTTTTAATCTTTTTTAACAATTTCCTTTTATATTTTTAACAATTTCCTTTTCTAATAATCTGATTTTATTAGTCTAGTCAAAAATGAAAAAACTAATTTCCTTAAATATATTTCGTATAATTTTACTTTTCAAAGTAATAATGATGATGATGATAAATAATTTAATAATAATGTAATTTTTATACAAATATATAATGCTCATTTATTTTATTATATAAATATTAATGATAATCATGATAAATAATTAATAATAATGTAATTTTTATACATATATATAATCATAGTTTATTTTATTATATAGATATTAGATTTAATTATATATTATTAATGTAGAATATTTTTATATTATGAACCAATTGTAATCATGATATATTAATTTTTTATCTTTTTATATTAGTTATAATTAAATTTAAAATTGCATATATGTTTGACTGTGATTTAAAATTTCTTGACAACGATTTAATCACAAAATTTATTGACGATTTATCAATTGAATTTATAAACATGTCTAATATATTTTCAACACAAATAATAATGTGACATGAGTAAAAAATAACAATTACATATGTTTTTTTAACTATTTCGTTTTACTATAAGAGGATTCATTCTTCCTATCAATTAACATAAGCTCATTTGTACTTGTATTTTACTCCCTCCGTCCCAAATATATTATCTCCGGACATATATTTTTGCGAACGTGACAATTATTATGATAAAGAGATTGTACTGTTTTATTAAAATTATCAAGAGATTGTACTGTCGTTATTAAAATTATCATTAATGTAATTTTGCCTTGGATTATTTATTTTAGGTGATGGAACCTTCAACTATAATGGAAGATGATCAATTTCAGCAAGAAACAGTGGCGCCACTGCCACCACCAACCCCCGCCGCATATCCATCTTCACATTATGGCGGCATCCCTGGGACACCTTTTCCGATGGGTCTTGGTCCGGTAACATTGCCGGTAATGAGTGCTGAAAGAGTGGCCAAGCCAATTAAGCCAACACCAATGCTGCCTCCTTCTTCTAAGATGGCTAATCTGAACTTGAAAGACAAAGCTGCTTCTTCTTCTTCCATCGAGCCTTTTCCATTGTCATTGAAGCTGCAACCTTCGGTTCCATCGGAAGATCATTCACCGGAAAGTAGCAGCCATTCGTCGTCATCGGCTTCATCATCGCCTTTTAAAACCATGGCTGCAGGGAAGTATAATGGTGGGGGTGGGGATAGCATTATCAGTGTTGCTTGA
Protein sequence

>MS.gene032707.t1

MTNLSQYEQPPPQDSNPADAGYVSDDIVHASGRSRERKRGVPWTEEEHKLFLLGLQQVGKGDWRGISRNFVKTRTPTQVASHAQKYFLRRHNQNRRRRRSSLFDITTDTVMEPSTIMEDDQFQQETVAPLPPPTPAAYPSSHYGGIPGTPFPMGLGPVTLPVMSAERVAKPIKPTPMLPPSSKMANLNLKDKAASSSSIEPFPLSLKLQPSVPSEDHSPESSSHSSSSASSSPFKTMAAGKYNGGGGDSIISVA