AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032789

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032789.t1 MTR_5g038280 95.195 333 7 1 1 333 1 324 0.0 644
MS.gene032789.t1 MTR_8g469430 53.030 330 94 8 10 333 12 286 2.90e-97 289
MS.gene032789.t1 MTR_4g107650 47.578 351 150 10 1 333 1 335 1.89e-79 246
MS.gene032789.t1 MTR_6g007647 47.208 197 90 3 87 280 33 218 1.20e-42 148
MS.gene032789.t1 MTR_6g007647 47.208 197 90 3 87 280 67 252 2.13e-42 149
MS.gene032789.t1 MTR_6g011610 43.388 242 95 10 39 261 41 259 1.23e-39 141
MS.gene032789.t1 MTR_6g011610 47.980 198 76 7 74 261 60 240 1.46e-39 140
MS.gene032789.t1 MTR_8g089895 64.815 108 37 1 77 183 43 150 7.39e-39 140
MS.gene032789.t1 MTR_3g086790 60.526 114 41 1 70 183 47 156 7.02e-38 137
MS.gene032789.t1 MTR_3g080100 66.327 98 33 0 87 184 20 117 2.35e-37 133
MS.gene032789.t1 MTR_3g080100 66.327 98 33 0 87 184 59 156 3.74e-37 134
MS.gene032789.t1 MTR_8g468210 66.667 96 32 0 87 182 77 172 8.05e-37 134
MS.gene032789.t1 MTR_5g039000 60.169 118 46 1 66 182 55 172 1.44e-36 133
MS.gene032789.t1 MTR_7g010020 58.559 111 44 1 74 182 65 175 3.90e-32 121
MS.gene032789.t1 MTR_1g061660 53.571 112 41 2 82 193 54 154 1.05e-26 105
MS.gene032789.t1 MTR_7g103340 55.670 97 36 1 87 183 63 152 1.21e-26 105
MS.gene032789.t1 MTR_3g092150 54.945 91 41 0 89 179 40 130 1.04e-25 103
MS.gene032789.t1 MTR_8g026960 47.573 103 53 1 91 193 38 139 1.75e-24 100
MS.gene032789.t1 MTR_5g019680 60.563 71 28 0 87 157 53 123 1.06e-19 86.3
MS.gene032789.t1 MTR_4g100550 45.902 122 53 4 67 186 103 213 3.23e-19 86.7
MS.gene032789.t1 MTR_5g019650 60.563 71 28 0 87 157 53 123 3.94e-19 85.1
MS.gene032789.t1 MTR_5g013010 40.645 155 71 5 37 185 89 228 1.42e-18 85.1
MS.gene032789.t1 MTR_5g014890 45.794 107 49 2 75 181 113 210 1.47e-17 81.6
MS.gene032789.t1 MTR_4g126900 46.154 91 42 1 90 180 91 174 1.95e-17 80.1
MS.gene032789.t1 MTR_8g006705 48.515 101 44 2 87 186 146 239 3.08e-17 81.3
MS.gene032789.t1 MTR_2g061030 44.340 106 52 1 75 180 188 286 4.33e-17 81.3
MS.gene032789.t1 MTR_7g093430 47.872 94 42 1 87 180 160 246 5.15e-17 80.5
MS.gene032789.t1 MTR_1g054285 46.809 94 43 1 87 180 154 240 3.19e-16 78.2
MS.gene032789.t1 MTR_4g097600 43.925 107 52 2 74 180 115 213 1.00e-15 76.3
MS.gene032789.t1 MTR_1g017090 46.316 95 44 1 87 181 130 217 1.82e-15 75.9
MS.gene032789.t1 MTR_4g084750 37.879 132 61 3 87 217 65 176 2.65e-15 73.9
MS.gene032789.t1 MTR_5g013010 41.667 108 50 3 37 139 89 188 6.84e-14 70.1
MS.gene032789.t1 MTR_3g103590 48.611 72 37 0 87 158 145 216 4.33e-13 69.3
MS.gene032789.t1 MTR_2g038625 41.489 94 48 1 87 180 82 168 2.77e-12 65.5
MS.gene032789.t1 MTR_2g038580 41.489 94 48 1 87 180 82 168 2.77e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032789.t1 AT3G01470 42.735 234 97 9 51 273 30 237 3.25e-42 147
MS.gene032789.t1 AT4G40060 54.615 130 53 2 76 202 45 171 6.00e-41 145
MS.gene032789.t1 AT2G22430 68.041 97 31 0 87 183 62 158 5.89e-39 140
MS.gene032789.t1 AT5G65310 68.041 97 31 0 87 183 54 150 2.47e-38 138
MS.gene032789.t1 AT5G65310 68.041 97 31 0 87 183 72 168 2.71e-38 138
MS.gene032789.t1 AT1G69780 64.545 110 38 1 74 182 71 180 2.74e-38 138
MS.gene032789.t1 AT3G01220 41.071 224 113 6 50 261 46 262 3.88e-37 135
MS.gene032789.t1 AT5G15150 60.000 115 44 1 66 180 96 208 2.28e-36 133
MS.gene032789.t1 AT1G26960 59.091 110 44 1 74 182 57 166 1.19e-33 125
MS.gene032789.t1 AT1G27045 46.939 147 60 3 37 183 36 164 1.11e-32 121
MS.gene032789.t1 AT1G27045 60.825 97 38 0 87 183 25 121 1.11e-31 117
MS.gene032789.t1 AT1G27045 60.825 97 38 0 87 183 25 121 1.11e-31 117
MS.gene032789.t1 AT1G27045 60.825 97 38 0 87 183 17 113 1.49e-31 117
MS.gene032789.t1 AT1G27045 46.259 147 60 4 37 183 36 163 1.97e-30 116
MS.gene032789.t1 AT2G46680 49.541 109 55 0 83 191 28 136 1.42e-28 111
MS.gene032789.t1 AT3G61890 51.429 105 50 1 90 194 33 136 1.79e-27 108
MS.gene032789.t1 AT2G46680 53.763 93 43 0 83 175 28 120 8.50e-27 106
MS.gene032789.t1 AT5G03790 53.608 97 38 1 87 183 77 166 2.08e-25 102
MS.gene032789.t1 AT5G03790 54.839 93 35 1 87 179 77 162 2.27e-24 98.6
MS.gene032789.t1 AT2G36610 47.222 108 42 2 84 183 68 168 7.92e-24 97.1
MS.gene032789.t1 AT4G36740 48.182 110 50 1 87 196 55 157 1.54e-22 94.4
MS.gene032789.t1 AT4G36740 48.182 110 50 1 87 196 56 158 1.66e-22 94.4
MS.gene032789.t1 AT2G18550 45.378 119 58 1 87 205 61 172 2.04e-22 94.0
MS.gene032789.t1 AT5G53980 46.392 97 52 0 87 183 11 107 6.85e-21 88.2
MS.gene032789.t1 AT5G66700 58.333 72 30 0 87 158 71 142 4.59e-20 87.8
MS.gene032789.t1 AT5G06710 48.421 95 42 1 87 181 190 277 3.71e-17 81.6
MS.gene032789.t1 AT4G17460 45.217 115 49 4 75 186 125 228 9.86e-17 79.7
MS.gene032789.t1 AT2G01430 39.706 136 64 4 50 180 103 225 1.98e-16 78.6
MS.gene032789.t1 AT5G47370 39.695 131 64 3 57 186 107 223 2.67e-16 78.2
MS.gene032789.t1 AT2G44910 47.000 100 45 2 87 185 163 255 4.06e-16 78.2
MS.gene032789.t1 AT3G60390 47.000 100 45 2 87 185 162 254 7.07e-16 77.4
MS.gene032789.t1 AT2G22800 45.263 95 45 1 87 181 113 200 9.17e-16 76.6
MS.gene032789.t1 AT4G37790 44.681 94 45 1 87 180 126 212 1.88e-15 75.9
MS.gene032789.t1 AT4G16780 46.000 100 46 2 87 185 129 221 2.57e-15 75.5
MS.gene032789.t1 AT5G06710 58.491 53 22 0 87 139 190 242 1.49e-12 67.4
MS.gene032789.t1 AT1G70920 43.299 97 48 1 84 180 66 155 1.80e-12 65.9
MS.gene032789.t1 AT1G70920 43.299 97 48 1 84 180 36 125 2.37e-12 65.1
MS.gene032789.t1 AT2G01430 41.053 95 45 3 50 139 103 191 3.76e-12 65.1

Find 51 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAAAATTCTTGTAATTT+TGG 0.015177 5.2:-28495603 MS.gene032789:CDS
TCAATTACAAATTCAGAAAA+TGG 0.161025 5.2:-28495894 MS.gene032789:CDS
CTGAAGCCCTTGAATCTCTT+TGG 0.177337 5.2:-28497273 MS.gene032789:CDS
TGAACCTTGCCCTTCTGTTT+TGG 0.204900 5.2:+28496550 None:intergenic
TTCTCTTTCTCTTTATCTCT+TGG 0.249115 5.2:+28495987 None:intergenic
GCAAGTTGAACTTTCCTATC+AGG 0.270065 5.2:+28496615 None:intergenic
TAGCAAACATCTTCAACTTT+TGG 0.273698 5.2:+28495636 None:intergenic
TGAAGCCCTTGAATCTCTTT+GGG 0.300479 5.2:-28497272 MS.gene032789:CDS
TGAGGTGAATTGATTGCTTC+TGG 0.314138 5.2:+28495945 None:intergenic
CAAAGTGTCACTCCCTAATA+TGG 0.317058 5.2:-28495868 MS.gene032789:CDS
AACCAAGGCAAGTTGCTATA+TGG 0.341312 5.2:-28496575 MS.gene032789:CDS
GTGGAAATTTAAATTCAATA+AGG 0.341810 5.2:+28495546 None:intergenic
TCTCTTTCTCTTTATCTCTT+GGG 0.342274 5.2:+28495988 None:intergenic
CAAAAGGTTTGATCTTCAAC+AGG 0.362492 5.2:+28495576 None:intergenic
TTTCTCAAGTTGTTGAAAGA+AGG 0.381022 5.2:+28496761 None:intergenic
GATAGCCCACATTGCAATGA+TGG 0.423802 5.2:-28495789 MS.gene032789:CDS
ATTGGATCTTTGTTGTTATG+AGG 0.430991 5.2:+28495927 None:intergenic
TTCTCAAGTTGTTGAAAGAA+GGG 0.453337 5.2:+28496762 None:intergenic
GTAATTGAGATTATATCCAT+TGG 0.466626 5.2:+28495909 None:intergenic
CATATTAGGGAGTGACACTT+TGG 0.480614 5.2:+28495869 None:intergenic
GTTCAACTTGCAAAAGAGCT+TGG 0.481261 5.2:-28496603 MS.gene032789:CDS
AACCATATAGCAACTTGCCT+TGG 0.481745 5.2:+28496573 None:intergenic
TGTGAGAAATCTGAATGATC+AGG 0.485138 5.2:+28495714 None:intergenic
CAATTACAAATTCAGAAAAT+GGG 0.485544 5.2:-28495893 MS.gene032789:CDS
GAATGACAAGTTAAAAGAAG+AGG 0.495747 5.2:-28496442 MS.gene032789:intron
TATAAGGCTGTTCTTATGGC+AGG 0.496797 5.2:-28497361 None:intergenic
TCAATAAGGCCAGAAACAAA+AGG 0.504416 5.2:+28495560 None:intergenic
AAAACAAGCTTGAACCTGAT+AGG 0.509669 5.2:-28496629 MS.gene032789:CDS
AAGAGCTTGGTTTGCAACCA+AGG 0.513381 5.2:-28496590 MS.gene032789:CDS
TGTGAACCCAAAGAGATTCA+AGG 0.525903 5.2:+28497266 None:intergenic
ACCATCAACAAGGAAAGAAA+AGG 0.538035 5.2:-28496701 MS.gene032789:CDS
AGATTTCTCACAAGATGAAG+AGG 0.546898 5.2:-28495703 MS.gene032789:CDS
TGTTCCCATCATTGCAATGT+GGG 0.548653 5.2:+28495784 None:intergenic
TCATAACAACAAAGATCCAA+TGG 0.563990 5.2:-28495925 MS.gene032789:CDS
GGATTCAGGTTCAAATTCAG+TGG 0.566281 5.2:-28496832 MS.gene032789:intron
TCAAGGGCTTCAGAAGTACA+AGG 0.569376 5.2:+28497283 None:intergenic
GTGAACCCAAAGAGATTCAA+GGG 0.581702 5.2:+28497267 None:intergenic
CTATATGGTTCCAAAACAGA+AGG 0.583686 5.2:-28496560 MS.gene032789:CDS
ATAGCCCACATTGCAATGAT+GGG 0.583931 5.2:-28495788 MS.gene032789:CDS
CTTGAGAAAGAAGAGAATTG+TGG 0.599550 5.2:-28496747 MS.gene032789:CDS
AAAGCATGTGAAGAATCTGT+AGG 0.619583 5.2:+28495741 None:intergenic
ATCAACAAGGAAAGAAAAGG+AGG 0.621166 5.2:-28496698 MS.gene032789:CDS
ATCACCTTGAAAAGAAGCAG+AGG 0.635119 5.2:+28497243 None:intergenic
TTGTTCCCATCATTGCAATG+TGG 0.645404 5.2:+28495783 None:intergenic
AAGGCTGTTCTTATGGCAGG+TGG 0.647915 5.2:-28497358 None:intergenic
GGTTCCAAAACAGAAGGGCA+AGG 0.651221 5.2:-28496554 MS.gene032789:CDS
TATATGGTTCCAAAACAGAA+GGG 0.680215 5.2:-28496559 MS.gene032789:CDS
AAAAGGTTTGATCTTCAACA+GGG 0.685076 5.2:+28495577 None:intergenic
GAAGCATGCTACCATCAACA+AGG 0.695864 5.2:-28496711 MS.gene032789:CDS
CAAGGGCTTCAGAAGTACAA+GGG 0.720149 5.2:+28497284 None:intergenic
TGGTGGTGGAAGCAACAGAG+TGG 0.722441 5.2:-28496796 MS.gene032789:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTTTGTTACAAT+AGG - chr5.2:28496870-28496889 MS.gene032789:intron 10.0%
!!! TAAAAAAGACTATTTTTTTA+TGG + chr5.2:28495966-28495985 None:intergenic 10.0%
!! TGAAAATTTACATTAGAATT+TGG + chr5.2:28496604-28496623 None:intergenic 15.0%
!!! ATGAAAATTCTTGTAATTTT+GGG - chr5.2:28497304-28497323 MS.gene032789:CDS 15.0%
!!! TGAAAAAGACTAATTTTATA+TGG + chr5.2:28496001-28496020 None:intergenic 15.0%
!! AATGAGTTAATGATTGAATA+TGG - chr5.2:28496830-28496849 MS.gene032789:intron 20.0%
!! AATGTGTATATTTGAATTGA+CGG - chr5.2:28495760-28495779 MS.gene032789:CDS 20.0%
!! ATGAGTTAATGATTGAATAT+GGG - chr5.2:28496831-28496850 MS.gene032789:intron 20.0%
!! CAAACTTTATTAAAGTAAAG+AGG + chr5.2:28496796-28496815 None:intergenic 20.0%
!! CAATTACAAATTCAGAAAAT+GGG - chr5.2:28497013-28497032 MS.gene032789:intron 20.0%
!! CAATTCAAATATACACATTA+TGG + chr5.2:28495759-28495778 None:intergenic 20.0%
!! CATATAGTTGAATTATAACA+TGG - chr5.2:28496034-28496053 MS.gene032789:intron 20.0%
!! TCAATTACAAATTCAGAAAA+TGG - chr5.2:28497012-28497031 MS.gene032789:intron 20.0%
!!! AAATTACAAGAATTTTCATG+TGG + chr5.2:28497303-28497322 None:intergenic 20.0%
!!! AATTACAAGAATTTTCATGT+GGG + chr5.2:28497302-28497321 None:intergenic 20.0%
!!! CATGAAAATTCTTGTAATTT+TGG - chr5.2:28497303-28497322 MS.gene032789:CDS 20.0%
!!! TACATTTTAGTACCATATTA+GGG + chr5.2:28497053-28497072 None:intergenic 20.0%
!!! TTACATTTTAGTACCATATT+AGG + chr5.2:28497054-28497073 None:intergenic 20.0%
! AAACAGCTTGAAAAAGATTA+TGG - chr5.2:28496384-28496403 MS.gene032789:intron 25.0%
! CAATAGAAAGTTTGTATTGA+CGG + chr5.2:28495837-28495856 None:intergenic 25.0%
! GTAATTGAGATTATATCCAT+TGG + chr5.2:28497000-28497019 None:intergenic 25.0%
! TCAATCATTAACTCATTAGT+GGG + chr5.2:28496827-28496846 None:intergenic 25.0%
! TGAGTTAATGATTGAATATG+GGG - chr5.2:28496832-28496851 MS.gene032789:intron 25.0%
! TTCAATCATTAACTCATTAG+TGG + chr5.2:28496828-28496847 None:intergenic 25.0%
! TTTCTTAAGATCATGAAAGT+GGG - chr5.2:28495917-28495936 MS.gene032789:CDS 25.0%
!! ATTTCTTGAAAAGAGTTTTG+AGG - chr5.2:28496251-28496270 MS.gene032789:intron 25.0%
AAAAGAAGTGAGAAGAATTG+AGG + chr5.2:28495697-28495716 None:intergenic 30.0%
AAAAGGTTTGATCTTCAACA+GGG + chr5.2:28497332-28497351 None:intergenic 30.0%
CGTCAATACAAACTTTCTAT+TGG - chr5.2:28495835-28495854 MS.gene032789:CDS 30.0%
GAATGACAAGTTAAAAGAAG+AGG - chr5.2:28496464-28496483 MS.gene032789:CDS 30.0%
GTCAACACATCAATAAATGT+TGG - chr5.2:28496562-28496581 MS.gene032789:CDS 30.0%
GTTTCTTAAGATCATGAAAG+TGG - chr5.2:28495916-28495935 MS.gene032789:CDS 30.0%
TATATGGTTCCAAAACAGAA+GGG - chr5.2:28496347-28496366 MS.gene032789:intron 30.0%
TCATAACAACAAAGATCCAA+TGG - chr5.2:28496981-28497000 MS.gene032789:intron 30.0%
TCTCTTTCTCTTTATCTCTT+GGG + chr5.2:28496921-28496940 None:intergenic 30.0%
TTCTCTTTCTCTTTATCTCT+TGG + chr5.2:28496922-28496941 None:intergenic 30.0%
! TAGCAAACATCTTCAACTTT+TGG + chr5.2:28497273-28497292 None:intergenic 30.0%
! TTCTCAAGTTGTTGAAAGAA+GGG + chr5.2:28496147-28496166 None:intergenic 30.0%
! TTTCTCAAGTTGTTGAAAGA+AGG + chr5.2:28496148-28496167 None:intergenic 30.0%
!! AAGATCAAACCTTTTGTTTC+TGG - chr5.2:28497337-28497356 MS.gene032789:CDS 30.0%
!! ATTGGATCTTTGTTGTTATG+AGG + chr5.2:28496982-28497001 None:intergenic 30.0%
!! TCAGTGGTTAATTTTGAGAA+TGG - chr5.2:28496090-28496109 MS.gene032789:intron 30.0%
!!! ATGTGTATGTGTGTTTTGAT+AGG + chr5.2:28496731-28496750 None:intergenic 30.0%
AAAACAAGCTTGAACCTGAT+AGG - chr5.2:28496277-28496296 MS.gene032789:intron 35.0%
AAAGCATGTGAAGAATCTGT+AGG + chr5.2:28497168-28497187 None:intergenic 35.0%
AATTGTCTGATCTCAATCCA+AGG - chr5.2:28496656-28496675 MS.gene032789:CDS 35.0%
ACCATCAACAAGGAAAGAAA+AGG - chr5.2:28496205-28496224 MS.gene032789:intron 35.0%
AGATTTCTCACAAGATGAAG+AGG - chr5.2:28497203-28497222 MS.gene032789:intron 35.0%
ATCAACAAGGAAAGAAAAGG+AGG - chr5.2:28496208-28496227 MS.gene032789:intron 35.0%
CAAAAGGTTTGATCTTCAAC+AGG + chr5.2:28497333-28497352 None:intergenic 35.0%
CTATATGGTTCCAAAACAGA+AGG - chr5.2:28496346-28496365 MS.gene032789:intron 35.0%
CTTGAGAAAGAAGAGAATTG+TGG - chr5.2:28496159-28496178 MS.gene032789:intron 35.0%
GTTAGTAAATCGATGATCCT+TGG + chr5.2:28496676-28496695 None:intergenic 35.0%
TCAATAAGGCCAGAAACAAA+AGG + chr5.2:28497349-28497368 None:intergenic 35.0%
TGTGAGAAATCTGAATGATC+AGG + chr5.2:28497195-28497214 None:intergenic 35.0%
TTGACACACACAAACGAAAT+TGG - chr5.2:28496503-28496522 MS.gene032789:CDS 35.0%
! ATGGTTTGTGTTTGATGACA+TGG - chr5.2:28496053-28496072 MS.gene032789:intron 35.0%
! TCCTTTTCTTTCCTTGTTGA+TGG + chr5.2:28496209-28496228 None:intergenic 35.0%
! TCTTCAACTTTTGGTAAGCA+AGG + chr5.2:28497264-28497283 None:intergenic 35.0%
!! GTGGTTAATTTTGAGAATGG+TGG - chr5.2:28496093-28496112 MS.gene032789:intron 35.0%
!! GTTAATTTTGAGAATGGTGG+TGG - chr5.2:28496096-28496115 MS.gene032789:intron 35.0%
AACCAAGGCAAGTTGCTATA+TGG - chr5.2:28496331-28496350 MS.gene032789:intron 40.0%
AACCATATAGCAACTTGCCT+TGG + chr5.2:28496336-28496355 None:intergenic 40.0%
AGAAGTGAGAAGAATTGAGG+AGG + chr5.2:28495694-28495713 None:intergenic 40.0%
ATAGCCCACATTGCAATGAT+GGG - chr5.2:28497118-28497137 MS.gene032789:intron 40.0%
ATCACCTTGAAAAGAAGCAG+AGG + chr5.2:28495666-28495685 None:intergenic 40.0%
CATATTAGGGAGTGACACTT+TGG + chr5.2:28497040-28497059 None:intergenic 40.0%
GTGAACCCAAAGAGATTCAA+GGG + chr5.2:28495642-28495661 None:intergenic 40.0%
GTTCAACTTGCAAAAGAGCT+TGG - chr5.2:28496303-28496322 MS.gene032789:intron 40.0%
TGAAGCCCTTGAATCTCTTT+GGG - chr5.2:28495634-28495653 MS.gene032789:CDS 40.0%
TGAGGTGAATTGATTGCTTC+TGG + chr5.2:28496964-28496983 None:intergenic 40.0%
TGTGAACCCAAAGAGATTCA+AGG + chr5.2:28495643-28495662 None:intergenic 40.0%
TGTTCCCATCATTGCAATGT+GGG + chr5.2:28497125-28497144 None:intergenic 40.0%
TTGTTCCCATCATTGCAATG+TGG + chr5.2:28497126-28497145 None:intergenic 40.0%
! CAAAGTGTCACTCCCTAATA+TGG - chr5.2:28497038-28497057 MS.gene032789:intron 40.0%
! GCAAGTTGAACTTTCCTATC+AGG + chr5.2:28496294-28496313 None:intergenic 40.0%
! GGATTCAGGTTCAAATTCAG+TGG - chr5.2:28496074-28496093 MS.gene032789:intron 40.0%
! GTGTTTGATGACATGGATTC+AGG - chr5.2:28496060-28496079 MS.gene032789:intron 40.0%
!! CACATCACTTTTAGCTGAGT+TGG + chr5.2:28497088-28497107 None:intergenic 40.0%
!!! TGTGTGTTTTGATAGGTGAC+CGG + chr5.2:28496724-28496743 None:intergenic 40.0%
CTGAAGCCCTTGAATCTCTT+TGG - chr5.2:28495633-28495652 MS.gene032789:CDS 45.0%
GAAGCATGCTACCATCAACA+AGG - chr5.2:28496195-28496214 MS.gene032789:intron 45.0%
GATAGCCCACATTGCAATGA+TGG - chr5.2:28497117-28497136 MS.gene032789:intron 45.0%
TTTGATAGGTGACCGGTGTT+CGG + chr5.2:28496717-28496736 None:intergenic 45.0%
! CAAGGGCTTCAGAAGTACAA+GGG + chr5.2:28495625-28495644 None:intergenic 45.0%
! CACACCTCTGCTTCTTTTCA+AGG - chr5.2:28495659-28495678 MS.gene032789:CDS 45.0%
! TCAAGGGCTTCAGAAGTACA+AGG + chr5.2:28495626-28495645 None:intergenic 45.0%
! TGAACCTTGCCCTTCTGTTT+TGG + chr5.2:28496359-28496378 None:intergenic 45.0%
!! AAGAGCTTGGTTTGCAACCA+AGG - chr5.2:28496316-28496335 MS.gene032789:intron 45.0%
GGTTCCAAAACAGAAGGGCA+AGG - chr5.2:28496352-28496371 MS.gene032789:intron 50.0%
! ATGGCAGGTGGCAAGCTTTT+TGG - chr5.2:28495560-28495579 MS.gene032789:CDS 50.0%
CATTCCGAGAGTCCGAACAC+CGG - chr5.2:28496702-28496721 MS.gene032789:CDS 55.0%
! GCAGGTGGCAAGCTTTTTGG+TGG - chr5.2:28495563-28495582 MS.gene032789:CDS 55.0%
!! TGGTGGTGGAAGCAACAGAG+TGG - chr5.2:28496110-28496129 MS.gene032789:intron 55.0%
GTGACCGGTGTTCGGACTCT+CGG + chr5.2:28496709-28496728 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 28495560 28497368 28495560 ID=MS.gene032789
chr5.2 mRNA 28495560 28497368 28495560 ID=MS.gene032789.t1;Parent=MS.gene032789
chr5.2 exon 28497248 28497368 28497248 ID=MS.gene032789.t1.exon1;Parent=MS.gene032789.t1
chr5.2 CDS 28497248 28497368 28497248 ID=cds.MS.gene032789.t1;Parent=MS.gene032789.t1
chr5.2 exon 28496443 28496846 28496443 ID=MS.gene032789.t1.exon2;Parent=MS.gene032789.t1
chr5.2 CDS 28496443 28496846 28496443 ID=cds.MS.gene032789.t1;Parent=MS.gene032789.t1
chr5.2 exon 28495560 28496036 28495560 ID=MS.gene032789.t1.exon3;Parent=MS.gene032789.t1
chr5.2 CDS 28495560 28496036 28495560 ID=cds.MS.gene032789.t1;Parent=MS.gene032789.t1
Gene Sequence

>MS.gene032789

ATGGCAGGTGGCAAGCTTTTTGGTGGTTCTAATATGTCACTTTTGCTTCAAAATGAAAGACTCCCTTGTACTTCTGAAGCCCTTGAATCTCTTTGGGTTCACACCTCTGCTTCTTTTCAAGGTGATCTTCACCTCCTCAATTCTTCTCACTTCTTTTGTTACATAATTGTCATCAGTTTTTGTAGTTGTTTCTTCACCATAATGTGTATATTTGAATTGACGGTGAGTTAGACAGAATCACTGATACACATAATCACTGATTCTGTTTAACTTACCGTCAATACAAACTTTCTATTGGTCTTAAAATCTCACAATTTTAAGTTTTATGAATATCATAGAGCATAACACACACATATGTTTCTTAAGATCATGAAAGTGGGTTTCTTTTTTCATTGTGAAGAAACCATAAAAAAATAGTCTTTTTTATTTTTGAAGAAACCATATAAAATTAGTCTTTTTCATTATTTTTCTATGCATATAGTTGAATTATAACATGGTTTGTGTTTGATGACATGGATTCAGGTTCAAATTCAGTGGTTAATTTTGAGAATGGTGGTGGAAGCAACAGAGTGGTAACAGATAGACCCTTCTTTCAACAACTTGAGAAAGAAGAGAATTGTGGTGATGAAGATTATGAAGCATGCTACCATCAACAAGGAAAGAAAAGGAGGCTTTCAAGTGAACAAGTTCAATTTCTTGAAAAGAGTTTTGAGGTAGAAAACAAGCTTGAACCTGATAGGAAAGTTCAACTTGCAAAAGAGCTTGGTTTGCAACCAAGGCAAGTTGCTATATGGTTCCAAAACAGAAGGGCAAGGTTCAAAACTAAACAGCTTGAAAAAGATTATGGCACATTGAAAGCTAGCTTTGATAGTCTCAAAGATGATTATGATAATCTTCTTCAAGAGAATGACAAGTTAAAAGAAGAGGTAAAAAAAGTAAAAAGTTGACACACACAAACGAAATTGGACACTATGTTGTCATTGATCGCACGCATTTCACACAGTCAACACATCAATAAATGTTGGATCGAGATCAGACAATCCAAATTCTAATGTAAATTTTCATCTTAAATCTAAAGTATCAAACTTTAAATTTGAATTGTCTGATCTCAATCCAAGGATCATCGATTTACTAACAGCGTGCATTCCGAGAGTCCGAACACCGGTCACCTATCAAAACACACATACACATAAAAACATACTAATTCAGCTTTTCTACTTTATGCTGCTATGACCTCTTTACTTTAATAAAGTTTGATATTTCTCCCACTAATGAGTTAATGATTGAATATGGGGTTAATTTTATTTTTTATTTTTTATTTTGTTACAATAGGTTAATTCTCTCAAGAACAAATTGATCCCAAGAGATAAAGAGAAAGAGAATTCAGATGAAAAATCATCACCAGAAGCAATCAATTCACCTCATAACAACAAAGATCCAATGGATATAATCTCAATTACAAATTCAGAAAATGGGTCCAAAGTGTCACTCCCTAATATGGTACTAAAATGTAAGCAAGAAGATGCCAACTCAGCTAAAAGTGATGTGCTTGATTCTGATAGCCCACATTGCAATGATGGGAACAATCTTTCTTCTTTCATAGAGCCTACAGATTCTTCACATGCTTTTGAACCTGATCATTCAGATTTCTCACAAGATGAAGAGGATAATGATAACTTGAGTCATAATCTTTTGACTCTTCCTTGCTTACCAAAAGTTGAAGATGTTTGCTATGATGACCCACATGAAAATTCTTGTAATTTTGGGTTCCCTGTTGAAGATCAAACCTTTTGTTTCTGGCCTTATTGA
Protein sequence

>MS.gene032789.t1

MAGGKLFGGSNMSLLLQNERLPCTSEALESLWVHTSASFQGSNSVVNFENGGGSNRVVTDRPFFQQLEKEENCGDEDYEACYHQQGKKRRLSSEQVQFLEKSFEVENKLEPDRKVQLAKELGLQPRQVAIWFQNRRARFKTKQLEKDYGTLKASFDSLKDDYDNLLQENDKLKEEVNSLKNKLIPRDKEKENSDEKSSPEAINSPHNNKDPMDIISITNSENGSKVSLPNMVLKCKQEDANSAKSDVLDSDSPHCNDGNNLSSFIEPTDSSHAFEPDHSDFSQDEEDNDNLSHNLLTLPCLPKVEDVCYDDPHENSCNFGFPVEDQTFCFWPY