AlfalfaGEDB | MS.gene032886

M. sativa cultivar XinJiangDaYe / MS.gene032886

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032886.t1	XP_003613753.1	93.3	401	16	2	1	400	1	391	5.80E-213	750

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032886.t1	Q9ZVH0	57.7	326	117	7	2	313	3	321	3.7e-91	336.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032886.t1	G7KDY3	93.3	401	16	2	1	400	1	391	4.2e-213	750.0

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene032886.t1	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032886.t1	MTR_5g040420	92.020	401	21	2	1	400	1	391	0.0	739
MS.gene032886.t1	MTR_8g467490	62.791	430	95	15	1	400	1	395	1.75e-171	484
MS.gene032886.t1	MTR_6g011860	46.189	433	158	16	1	400	1	391	1.05e-113	338
MS.gene032886.t1	MTR_5g090970	53.390	236	96	5	6	234	10	238	5.25e-79	246
MS.gene032886.t1	MTR_5g090970	52.966	236	96	6	6	234	10	237	1.39e-76	240
MS.gene032886.t1	MTR_2g068880	64.740	173	53	4	6	172	16	186	1.81e-74	234
MS.gene032886.t1	MTR_2g068920	62.857	175	51	4	11	175	18	188	1.03e-72	229
MS.gene032886.t1	MTR_4g089135	50.279	179	72	2	14	176	15	192	1.67e-60	199
MS.gene032886.t1	MTR_2g080010	51.786	168	68	2	16	175	17	179	1.44e-59	197
MS.gene032886.t1	MTR_4g134460	54.938	162	62	3	12	173	52	202	1.84e-59	199

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032886.t1	AT1G26870	55.828	326	123	7	2	313	3	321	7.48e-110	329
MS.gene032886.t1	AT5G39820	68.342	199	49	3	2	186	8	206	9.71e-99	298
MS.gene032886.t1	AT2G43000	64.118	170	52	2	6	175	10	170	1.08e-75	236
MS.gene032886.t1	AT3G12910	46.094	256	128	5	6	254	13	265	2.09e-66	213
MS.gene032886.t1	AT2G02450	55.625	160	60	3	13	172	50	198	8.01e-60	200
MS.gene032886.t1	AT3G04070	53.672	177	71	4	16	184	12	185	6.79e-59	195
MS.gene032886.t1	AT3G15510	52.096	167	67	2	18	176	21	182	7.32e-58	193
MS.gene032886.t1	AT1G61110	54.268	164	70	2	16	175	18	180	1.01e-56	189
MS.gene032886.t1	AT3G04070	49.223	193	71	4	16	184	12	201	1.96e-56	189
MS.gene032886.t1	AT1G01720	52.121	165	72	2	16	180	9	166	1.12e-55	185

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Find 83 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACTAAGGATTTCTTT+AGG	0.389485	5.4:-30101995	None:intergenic
AAACAGTGCAAGGCCTAATA+GGG	0.452535	5.4:+30101896	MS.gene032886:CDS
AAACCTATCTACTCCTTAGA+AGG	0.422338	5.4:+30101957	MS.gene032886:CDS
AAAGAGCTTTGTCAAACTCT+TGG	0.374630	5.4:+30102743	MS.gene032886:CDS
AAGAGCTTTGTCAAACTCTT+GGG	0.395123	5.4:+30102744	MS.gene032886:CDS
AAGTACTTCTTTGGTGAAAG+TGG	0.447802	5.4:-30102092	None:intergenic
AAGTTATCTTACCAGGGTTT+AGG	0.305652	5.4:+30101238	MS.gene032886:CDS
AATGACTACTCATTACCATT+TGG	0.401669	5.4:+30103198	MS.gene032886:CDS
AATGCACTTGGAACCTTCTA+AGG	0.302687	5.4:-30101970	None:intergenic
AATTCTTGTTCATCAATACG+AGG	0.699073	5.4:-30103282	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGACTACTCATTACCATT+TGG	+	chr5.4:30103198-30103217	MS.gene032886:CDS	30.0%
AATTCTTGTTCATCAATACG+AGG	-	chr5.4:30103285-30103304	None:intergenic	30.0%
ACACTGTTTAGCATAGTTTA+TGG	-	chr5.4:30101772-30101791	None:intergenic	30.0%
ACTACAAATTTCATGAATGG+TGG	+	chr5.4:30103159-30103178	MS.gene032886:CDS	30.0%
CATTACGATCCTTGATATTA+TGG	+	chr5.4:30102194-30102213	MS.gene032886:intron	30.0%
CATTGTCAACATATCATAGT+TGG	+	chr5.4:30102462-30102481	MS.gene032886:intron	30.0%
CTACAAATTTCATGAATGGT+GGG	+	chr5.4:30103160-30103179	MS.gene032886:CDS	30.0%
TAAAGAGAAAGGTTCAACAA+AGG	+	chr5.4:30101298-30101317	MS.gene032886:CDS	30.0%
TAAAGTTTACTCACATTAGC+AGG	-	chr5.4:30102131-30102150	None:intergenic	30.0%
TAACATCTACATCAAAGTCT+TGG	-	chr5.4:30102906-30102925	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	30101204	30103413	30101204	ID=MS.gene032886
chr5.4	mRNA	30101204	30103413	30101204	ID=MS.gene032886.t1;Parent=MS.gene032886
chr5.4	exon	30101204	30101384	30101204	ID=MS.gene032886.t1.exon1;Parent=MS.gene032886.t1
chr5.4	CDS	30101204	30101384	30101204	ID=cds.MS.gene032886.t1;Parent=MS.gene032886.t1
chr5.4	exon	30101835	30102136	30101835	ID=MS.gene032886.t1.exon2;Parent=MS.gene032886.t1
chr5.4	CDS	30101835	30102136	30101835	ID=cds.MS.gene032886.t1;Parent=MS.gene032886.t1
chr5.4	exon	30102694	30103413	30102694	ID=MS.gene032886.t1.exon3;Parent=MS.gene032886.t1
chr5.4	CDS	30102694	30103413	30102694	ID=cds.MS.gene032886.t1;Parent=MS.gene032886.t1

Gene Sequence

>MS.gene032886

ATGGAAGAAAGAAATGATGGTGAAAAACTTGATGAAGTTATCTTACCAGGGTTTAGGTTTCATCCAACTGATGAAGAGCTTGTTGGGTTTTACCTAAAGAGAAAGGTTCAACAAAGGCCTCTGTCTATTGAGCTCATTAAGCAGCTTGATATCTATAAATATGATCCTTGGGATCTTCCAAGTAAGACAAGACACTTATATATCTATGTTGATTCTTCACATTTATAACCCTTTATAAGCATTTTTTTTCAAACTGAACTTAGCCTCTTTAGGTAAACAACTTAATTAAGTGCTTATAGTATACACACTTACCATATAAATACCTATATATAAGATATTTCTATTACAAAAGTCAAACTGGTTCGATATAGGCTTGCATACTAAAGAAGGTTGATAATAGTTAAGGTGCTAACCTAGTTGGGATGTTCGCACATTACATTAATGTTTTGCATGTCTAACTTTTATCAACAAAAGAAGTCATAAGTTGTTTACATAAGTTATCCTAGAGAGCTTATAGAAATAAACGAAAAAAAGTTTAAGACTTGTCATAAGCAATTTCCACTTTCCATAAACTATGCTAAACAGTGTCACAAGTACTAATATATATGTTTGTATGATTGTATGTGTGTAGAGTTTGCAAGTACAGGAGAGAAAGAGTGGTATTTCTACTGTCCAAGAGACAGAAAATACAGAAACAGTGCAAGGCCTAATAGGGTAACTGGAGCTGGATTCTGGAAAGCTACAGGGACAGATAAACCTATCTACTCCTTAGAAGGTTCCAAGTGCATTGGCCTAAAGAAATCCTTAGTCTTTTACAAAGGAAGAGCTGCTAAAGGAATCAAAACTGACTGGATGATGCATGAGTTCAGGCTACCTTCTCTCATAGATCCACTTTCACCAAAGAAGTACTTGGAGAAAACCATTCCTGCTAATGTGAGTAAACTTTATCATTATCTTAGGAAATTTAAGTTATCATTTTCAATGCAATCACATTACGATCCTTGATATTATGGAGAATCGCAGTCAAATGTAACTCGTGCGATGTCAACATAGGTTGCATGAACTCCCTCTAATTCTAAGTATATTTTTATGAAAATGATTAGTCTATGTTGAAATATGTAGTTAAAAATTGTGTGCCTAAAATATCTATATTAATAATAAATGTTGTATTTGTATTCTAAAGTGAGAAATAATTTGAGACAAATATTTTAAATGAAATGGTTGCAATGAGATGGAGGGAGTATCAAAAACTTGTTGACATTGTCAACATATCATAGTTGGTCATATGTGTGACTTTAAAAATAGTTATGAATAAAGTCAAACATTTTTAATGATTTTAGCGGTTATAATTTATTGACCGATACAACATTCTGTACGGTGTGATGTATATGAATTTTTTTTTTTTTAAAAAACTTAGGTTTTAATTGTTAGTGTTTGTAAATATCATTTATGGAATTATGGACATCTTTAACTTGTCTTTTAACATGCAGGAATCATGGGCTATCTGTAGAATATTCAAGAAAACAAATTCTACACCTCAAAGAGCTTTGTCAAACTCTTGGGTTTCTTCCCTATCTGAAACAAGAACCTCTAATATGCTAACCAAAAATCAAGAAATCACCCAATTTTGTTCAAACAACATGTCATTGACAAAGAGTACATCCTTAACCAACCACTTCTACACTAACAACAATGATAACCAAGACTTTGATGTAGATGTTACCCCTTATAAATCCACTATTAGTCCATTACTACACAAAGCCTTTCATCACTTGCCCATTTCAAATGAAGATCTTAGTACTAACTTGATATTCTCATCATCATCTCCACTTGAAACATCTAACACTAATAGTGATATTATTTCCAAATCTACAATGGATGTTTCATCAATGTTGTTGAATATGTCATCATCCTCAATGCTTGGTGACTTTAACAAGACATGTGATGAGGTTACAACTACAAATTTCATGAATGGTGGGTTGCAAGAGCAATTTAATGACTACTCATTACCATTTGGTAATTACCAATATGATAATAATGCATTGGTTAAGGTACCTTATAATGTTAATGTGAATGTGCCTCGTATTGATGAACAAGAATTGATGAATTTCAATATTGGTGATGCATGGAAGTCAAATTTGCTTTGGGATACTTCATTTTGTCCTATTGATGTTCCCTCTAGTTATTCAACAACCAAGTGTTACACTTAA

Protein sequence

>MS.gene032886.t1

MEERNDGEKLDEVILPGFRFHPTDEELVGFYLKRKVQQRPLSIELIKQLDIYKYDPWDLPKFASTGEKEWYFYCPRDRKYRNSARPNRVTGAGFWKATGTDKPIYSLEGSKCIGLKKSLVFYKGRAAKGIKTDWMMHEFRLPSLIDPLSPKKYLEKTIPANESWAICRIFKKTNSTPQRALSNSWVSSLSETRTSNMLTKNQEITQFCSNNMSLTKSTSLTNHFYTNNNDNQDFDVDVTPYKSTISPLLHKAFHHLPISNEDLSTNLIFSSSSPLETSNTNSDIISKSTMDVSSMLLNMSSSSMLGDFNKTCDEVTTTNFMNGGLQEQFNDYSLPFGNYQYDNNALVKVPYNVNVNVPRIDEQELMNFNIGDAWKSNLLWDTSFCPIDVPSSYSTTKCYT