AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032930.t1 MTR_5g041350 87.535 353 43 1 1 352 1 353 0.0 610
MS.gene032930.t1 MTR_5g041350 85.171 263 38 1 91 352 2 264 3.73e-152 429
MS.gene032930.t1 MTR_5g041350 85.950 242 33 1 112 352 1 242 2.02e-140 398
MS.gene032930.t1 MTR_4g081710 58.857 350 128 5 2 349 4 339 2.61e-128 371
MS.gene032930.t1 MTR_2g086450 50.612 245 103 5 8 244 38 272 1.85e-69 222
MS.gene032930.t1 MTR_6g032990 48.696 230 111 3 8 233 35 261 1.22e-62 205
MS.gene032930.t1 MTR_6g444980 56.688 157 59 3 8 164 28 175 2.19e-49 170
MS.gene032930.t1 MTR_5g027440 44.865 185 94 4 5 186 15 194 1.48e-45 157
MS.gene032930.t1 MTR_6g444980 52.795 161 67 3 8 168 28 179 4.78e-45 157
MS.gene032930.t1 MTR_2g027800 42.797 236 114 7 8 230 32 259 5.10e-43 151
MS.gene032930.t1 MTR_7g088070 51.724 145 61 2 14 158 49 184 8.58e-43 150
MS.gene032930.t1 MTR_7g088070 51.724 145 57 3 14 158 49 180 6.70e-42 147
MS.gene032930.t1 MTR_2g027800 42.060 233 106 7 8 230 32 245 1.20e-41 147
MS.gene032930.t1 MTR_1g090670 50.704 142 67 1 8 149 37 175 8.15e-40 142
MS.gene032930.t1 MTR_2g027860 49.032 155 68 3 15 169 49 192 6.86e-39 141
MS.gene032930.t1 MTR_7g098250 44.218 147 66 2 11 155 258 390 1.28e-36 138
MS.gene032930.t1 MTR_7g115530 38.298 188 92 3 15 200 218 383 7.64e-36 135
MS.gene032930.t1 MTR_1g080330 44.375 160 71 5 11 168 171 314 2.74e-35 132
MS.gene032930.t1 MTR_1g053835 38.926 149 77 1 11 159 254 388 2.49e-32 125
MS.gene032930.t1 MTR_7g093030 36.306 157 87 3 14 170 211 354 5.58e-30 118
MS.gene032930.t1 MTR_1g080330 45.238 126 53 4 11 134 171 282 2.12e-27 109
MS.gene032930.t1 MTR_7g115530 44.355 124 53 2 14 135 217 326 3.96e-27 109
MS.gene032930.t1 MTR_7g068600 39.310 145 73 4 11 152 9 141 3.36e-24 99.8
MS.gene032930.t1 MTR_1g053800 38.926 149 72 2 8 153 170 302 1.15e-22 97.1
MS.gene032930.t1 MTR_7g115530 52.439 82 37 1 14 93 217 298 4.98e-22 95.1
MS.gene032930.t1 MTR_7g115530 60.345 58 23 0 14 71 217 274 7.20e-21 92.8
MS.gene032930.t1 MTR_7g115530 63.462 52 19 0 15 66 218 269 2.46e-19 87.0
MS.gene032930.t1 MTR_7g093030 55.172 58 26 0 14 71 211 268 3.13e-19 87.0
MS.gene032930.t1 MTR_1g053830 49.180 61 31 0 11 71 14 74 7.53e-19 80.9
MS.gene032930.t1 MTR_7g115530 60.377 53 21 0 15 67 218 270 1.80e-18 84.7
MS.gene032930.t1 MTR_3g450310 54.098 61 28 0 15 75 19 79 3.70e-17 79.7
MS.gene032930.t1 MTR_0223s0040 51.316 76 34 1 16 88 19 94 5.19e-17 80.5
MS.gene032930.t1 MTR_1g090670 44.660 103 54 1 47 149 1 100 7.31e-17 79.0
MS.gene032930.t1 MTR_1g112370 47.191 89 37 3 16 99 23 106 9.61e-17 80.5
MS.gene032930.t1 MTR_2g023580 53.333 60 28 0 16 75 56 115 1.75e-16 78.2
MS.gene032930.t1 MTR_7g069660 54.717 53 24 0 16 68 72 124 2.74e-16 79.3
MS.gene032930.t1 MTR_4g021790 60.000 55 21 1 15 69 168 221 3.60e-16 78.2
MS.gene032930.t1 MTR_2g016220 42.391 92 44 3 7 98 83 165 1.07e-15 77.8
MS.gene032930.t1 MTR_2g016220 42.391 92 44 3 7 98 83 165 1.12e-15 77.8
MS.gene032930.t1 MTR_5g029470 46.154 78 42 0 16 93 220 297 1.93e-15 77.4
MS.gene032930.t1 MTR_6g071625 43.182 88 49 1 16 102 203 290 2.86e-15 76.6
MS.gene032930.t1 MTR_2g016220 39.785 93 49 3 7 98 83 169 4.02e-15 76.3
MS.gene032930.t1 MTR_8g086410 54.717 53 24 0 16 68 112 164 4.33e-15 76.3
MS.gene032930.t1 MTR_8g086410 54.717 53 24 0 16 68 110 162 4.54e-15 76.3
MS.gene032930.t1 MTR_2g084230 54.717 53 24 0 16 68 98 150 6.79e-15 75.5
MS.gene032930.t1 MTR_2g084230 54.717 53 24 0 16 68 98 150 7.47e-15 74.7
MS.gene032930.t1 MTR_4g121020 59.322 59 23 1 11 69 211 268 8.41e-15 75.9
MS.gene032930.t1 MTR_4g121020 59.322 59 23 1 11 69 211 268 8.96e-15 75.9
MS.gene032930.t1 MTR_4g121020 59.322 59 23 1 11 69 107 164 9.31e-15 75.9
MS.gene032930.t1 MTR_4g021855 58.182 55 22 1 15 69 168 221 1.50e-14 73.6
MS.gene032930.t1 MTR_2g034960 55.714 70 24 2 11 74 207 275 1.54e-14 75.1
MS.gene032930.t1 MTR_5g017980 61.818 55 21 0 15 69 193 247 2.31e-14 73.6
MS.gene032930.t1 MTR_8g066310 46.479 71 38 0 16 86 174 244 2.69e-14 73.6
MS.gene032930.t1 MTR_8g086410 46.154 65 31 1 16 76 112 176 3.07e-14 73.9
MS.gene032930.t1 MTR_3g064840 54.386 57 26 0 16 72 275 331 5.66e-14 73.2
MS.gene032930.t1 MTR_1g021520 40.909 88 44 1 16 95 127 214 6.56e-14 71.6
MS.gene032930.t1 MTR_1g032570 55.932 59 25 1 11 69 127 184 7.15e-14 73.2
MS.gene032930.t1 MTR_1g032570 55.932 59 25 1 11 69 196 253 7.91e-14 72.8
MS.gene032930.t1 MTR_4g021760 57.407 54 22 1 15 68 169 221 8.51e-14 71.6
MS.gene032930.t1 MTR_4g086835 56.897 58 25 0 12 69 271 328 8.96e-14 72.4
MS.gene032930.t1 MTR_7g089010 58.182 55 22 1 15 69 91 144 1.22e-13 70.9
MS.gene032930.t1 MTR_4g064730 55.172 58 25 1 12 69 128 184 1.74e-13 70.9
MS.gene032930.t1 MTR_2g450070 50.847 59 28 1 11 69 209 266 3.44e-13 71.2
MS.gene032930.t1 MTR_2g450070 50.847 59 28 1 11 69 209 266 3.54e-13 70.9
MS.gene032930.t1 MTR_4g113140 55.172 58 26 0 12 69 232 289 4.25e-13 70.5
MS.gene032930.t1 MTR_4g021845 58.824 51 20 1 15 65 168 217 4.69e-13 68.2
MS.gene032930.t1 MTR_1g093080 61.818 55 21 0 15 69 211 265 5.14e-13 69.7
MS.gene032930.t1 MTR_5g017980 61.818 55 20 1 15 69 193 246 5.62e-13 69.3
MS.gene032930.t1 MTR_5g017980 61.818 55 20 1 15 69 192 245 5.74e-13 69.3
MS.gene032930.t1 MTR_4g086835 56.897 58 24 1 12 69 271 327 1.31e-12 68.9
MS.gene032930.t1 MTR_7g093010 32.847 137 79 3 15 150 213 337 1.88e-12 68.2
MS.gene032930.t1 MTR_4g131600 50.847 59 28 1 11 69 197 254 1.42e-11 65.9
MS.gene032930.t1 MTR_5g054300 56.364 55 24 0 15 69 198 252 1.81e-11 65.1
MS.gene032930.t1 MTR_7g026400 50.909 55 26 1 15 69 157 210 2.56e-11 65.1
MS.gene032930.t1 MTR_5g014040 45.455 66 35 1 17 82 313 377 6.70e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032930.t1 AT1G69580 50.000 266 116 5 6 262 25 282 1.94e-76 239
MS.gene032930.t1 AT1G69580 49.438 267 117 5 6 262 25 283 2.94e-75 236
MS.gene032930.t1 AT3G04030 53.648 233 98 4 8 235 38 265 3.50e-68 219
MS.gene032930.t1 AT5G18240 51.931 233 102 4 8 235 38 265 3.78e-67 217
MS.gene032930.t1 AT5G18240 51.931 233 102 4 8 235 38 265 3.78e-67 217
MS.gene032930.t1 AT3G04030 52.790 233 99 5 8 235 38 264 4.46e-65 211
MS.gene032930.t1 AT5G18240 51.073 233 102 5 8 235 38 263 8.72e-64 208
MS.gene032930.t1 AT3G04030 51.502 233 97 5 8 235 38 259 2.58e-61 201
MS.gene032930.t1 AT5G18240 49.785 233 101 5 8 235 38 259 2.26e-60 199
MS.gene032930.t1 AT5G18240 49.785 233 101 5 8 235 38 259 2.26e-60 199
MS.gene032930.t1 AT3G24120 46.018 226 114 4 8 226 34 258 3.30e-57 188
MS.gene032930.t1 AT4G13640 45.726 234 105 6 8 229 30 253 1.14e-56 186
MS.gene032930.t1 AT3G24120 45.415 229 114 5 8 226 34 261 5.62e-55 182
MS.gene032930.t1 AT4G13640 45.148 237 105 7 8 229 30 256 1.51e-54 181
MS.gene032930.t1 AT1G79430 57.325 157 58 3 8 164 27 174 2.03e-51 175
MS.gene032930.t1 AT5G45580 43.902 164 86 2 9 166 1 164 7.79e-42 147
MS.gene032930.t1 AT5G45580 43.902 164 86 2 9 166 17 180 1.01e-41 147
MS.gene032930.t1 AT3G12730 49.351 154 70 3 8 159 16 163 1.05e-40 143
MS.gene032930.t1 AT2G01060 49.351 154 66 4 14 167 14 155 1.32e-40 144
MS.gene032930.t1 AT3G04030 48.454 194 90 4 47 235 1 189 1.40e-40 145
MS.gene032930.t1 AT3G04450 43.243 148 75 2 11 158 194 332 9.24e-36 134
MS.gene032930.t1 AT3G04450 43.243 148 75 2 11 158 194 332 9.24e-36 134
MS.gene032930.t1 AT5G29000 44.000 150 65 3 14 158 187 322 1.47e-35 133
MS.gene032930.t1 AT5G29000 44.000 150 65 3 14 158 187 322 1.47e-35 133
MS.gene032930.t1 AT3G04450 43.243 148 75 2 11 158 234 372 1.48e-35 134
MS.gene032930.t1 AT5G29000 44.000 150 65 3 14 158 230 365 2.54e-35 133
MS.gene032930.t1 AT4G28610 42.568 148 74 2 14 161 224 360 8.20e-35 132
MS.gene032930.t1 AT3G04030 45.876 194 89 5 47 235 1 183 4.97e-34 127
MS.gene032930.t1 AT5G18240 44.845 194 91 5 47 235 1 183 5.94e-34 127
MS.gene032930.t1 AT5G06800 41.667 144 76 2 15 158 192 327 7.28e-32 123
MS.gene032930.t1 AT5G06800 40.972 144 72 2 15 158 192 322 3.98e-31 121
MS.gene032930.t1 AT3G13040 43.357 143 74 2 11 153 237 372 2.48e-30 120
MS.gene032930.t1 AT3G13040 43.357 143 74 2 11 153 237 372 2.48e-30 120
MS.gene032930.t1 AT3G13040 43.357 143 74 2 11 153 237 372 2.48e-30 120
MS.gene032930.t1 AT2G20400 42.484 153 67 3 15 162 231 367 7.90e-30 118
MS.gene032930.t1 AT2G20400 41.830 153 67 3 15 162 231 366 2.31e-29 117
MS.gene032930.t1 AT1G79430 53.390 118 46 3 47 164 1 109 4.00e-28 111
MS.gene032930.t1 AT3G04450 57.377 61 26 0 11 71 234 294 5.53e-21 92.4
MS.gene032930.t1 AT2G01060 45.763 118 52 4 50 167 1 106 7.07e-21 90.5
MS.gene032930.t1 AT5G29000 56.897 58 25 0 14 71 230 287 1.71e-20 90.9
MS.gene032930.t1 AT2G06020 55.738 61 27 0 11 71 82 142 1.81e-18 85.1
MS.gene032930.t1 AT5G06800 56.140 57 25 0 15 71 192 248 3.02e-18 84.0
MS.gene032930.t1 AT1G14600 60.377 53 21 0 16 68 24 76 8.96e-18 82.4
MS.gene032930.t1 AT3G12730 43.478 115 57 3 47 159 1 109 1.22e-17 80.1
MS.gene032930.t1 AT2G02060 54.098 61 28 0 16 76 30 90 5.42e-17 80.1
MS.gene032930.t1 AT2G02060 54.098 61 28 0 16 76 30 90 5.42e-17 80.1
MS.gene032930.t1 AT1G32240 36.066 122 73 2 16 132 214 335 3.00e-16 79.7
MS.gene032930.t1 AT1G32240 32.374 139 88 3 16 149 214 351 3.43e-16 79.3
MS.gene032930.t1 AT4G04605 54.717 53 24 0 16 68 15 67 1.27e-15 75.5
MS.gene032930.t1 AT2G38300 54.717 53 24 0 16 68 55 107 2.28e-15 76.6
MS.gene032930.t1 AT2G40260 52.542 59 28 0 16 74 83 141 6.18e-15 75.9
MS.gene032930.t1 AT2G42660 50.000 64 29 1 16 79 51 111 7.47e-15 73.9
MS.gene032930.t1 AT4G17695 46.835 79 36 1 16 88 165 243 1.14e-14 74.3
MS.gene032930.t1 AT5G16560 54.386 57 26 0 16 72 220 276 1.90e-14 74.3
MS.gene032930.t1 AT5G59570 55.172 58 25 1 12 69 138 194 2.23e-14 73.2
MS.gene032930.t1 AT5G59570 55.172 58 25 1 12 69 138 194 2.23e-14 73.2
MS.gene032930.t1 AT2G40970 56.897 58 24 1 12 69 102 158 3.45e-14 72.0
MS.gene032930.t1 AT5G05090 58.182 55 22 1 15 69 81 134 3.68e-14 72.0
MS.gene032930.t1 AT3G10760 58.182 55 22 1 15 69 105 158 4.91e-14 72.8
MS.gene032930.t1 AT5G42630 53.571 56 26 0 16 71 106 161 5.14e-14 70.9
MS.gene032930.t1 AT3G16857 58.182 55 22 1 15 69 167 220 6.45e-14 73.2
MS.gene032930.t1 AT3G16857 58.182 55 22 1 15 69 237 290 7.23e-14 73.2
MS.gene032930.t1 AT3G16857 58.182 55 22 1 15 69 237 290 7.35e-14 73.2
MS.gene032930.t1 AT5G42630 53.571 56 26 0 16 71 106 161 7.47e-14 71.2
MS.gene032930.t1 AT3G46640 56.364 55 23 1 15 69 144 197 7.79e-14 72.0
MS.gene032930.t1 AT3G46640 56.364 55 23 1 15 69 144 197 7.79e-14 72.0
MS.gene032930.t1 AT3G46640 56.364 55 23 1 15 69 144 197 8.38e-14 72.0
MS.gene032930.t1 AT1G25550 56.897 58 25 0 12 69 206 263 2.36e-13 70.9
MS.gene032930.t1 AT1G49560 60.000 55 22 0 15 69 192 246 2.82e-13 70.5
MS.gene032930.t1 AT3G25790 50.794 63 31 0 20 82 201 263 4.35e-13 70.1
MS.gene032930.t1 AT1G68670 58.621 58 24 0 12 69 212 269 4.65e-13 69.7
MS.gene032930.t1 AT4G04580 47.059 68 35 1 1 68 1 67 8.08e-13 66.2
MS.gene032930.t1 AT2G03500 53.448 58 27 0 12 69 230 287 1.90e-12 68.6
MS.gene032930.t1 AT4G37180 54.717 53 24 0 17 69 212 264 2.02e-12 67.8
MS.gene032930.t1 AT4G37180 54.717 53 24 0 17 69 219 271 2.35e-12 67.8
MS.gene032930.t1 AT2G01760 52.727 55 25 1 15 69 135 188 6.51e-12 66.2
MS.gene032930.t1 AT2G01760 52.727 55 25 1 15 69 200 253 9.94e-12 65.9
MS.gene032930.t1 AT1G67710 47.059 68 31 1 17 79 121 188 1.11e-11 66.2
MS.gene032930.t1 AT5G58080 52.542 59 27 1 11 69 190 247 1.28e-11 66.2
MS.gene032930.t1 AT2G01760 52.727 55 25 1 15 69 228 281 1.32e-11 65.9
MS.gene032930.t1 AT1G67710 47.059 68 31 1 17 79 195 262 1.51e-11 65.9
MS.gene032930.t1 AT1G67710 47.059 68 31 1 17 79 130 197 1.57e-11 65.9
MS.gene032930.t1 AT1G13300 47.761 67 35 0 15 81 181 247 1.66e-11 65.1
MS.gene032930.t1 AT3G62670 48.387 62 28 1 11 68 207 268 3.19e-11 64.7
MS.gene032930.t1 AT5G44190 51.852 54 25 1 15 68 147 199 3.26e-11 64.3
MS.gene032930.t1 AT5G45580 33.636 110 67 2 63 166 16 125 3.40e-11 62.8
MS.gene032930.t1 AT5G44190 51.852 54 25 1 15 68 147 199 4.50e-11 63.9
MS.gene032930.t1 AT1G49560 50.000 64 30 1 15 78 192 253 6.92e-11 62.4
MS.gene032930.t1 AT1G25550 53.704 54 25 0 12 65 206 259 6.99e-11 62.8

Find 61 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACTCAGTGATTTGATTC+TGG 0.268350 5.4:+28119296 None:intergenic
TGGAACTTCTTTGTGTTCAT+TGG 0.299674 5.4:-28118613 MS.gene032930:CDS
ATCACTCTCATAATACTCTT+TGG 0.312893 5.4:+28119822 None:intergenic
TTCACTTGAATTGCCACTAA+TGG 0.314380 5.4:-28118502 MS.gene032930:CDS
CAGAGTAGGTTTCTAGTTGT+TGG 0.316123 5.4:+28119641 None:intergenic
ATGTTGAGAATTCTTCATTA+AGG 0.317661 5.4:-28118356 MS.gene032930:CDS
AAAATGGGAATACCTGTAAA+TGG 0.318364 5.4:+28119763 None:intergenic
TAATTGCATCAATGAATCTT+TGG 0.326033 5.4:+28119990 None:intergenic
AAACATTCAATAGAAGAAAT+AGG 0.327972 5.4:-28118549 MS.gene032930:CDS
AGTCCTTGTCATTCTGATAT+TGG 0.344487 5.4:+28118274 None:intergenic
ATGAATCTTTGGTGAAGTTC+AGG 0.353886 5.4:+28120001 None:intergenic
CCATCTCAAGAGCCATTTAC+AGG 0.357774 5.4:-28119775 MS.gene032930:intron
AATGAAGAAACTAAATTTGA+TGG 0.358160 5.4:-28118384 MS.gene032930:CDS
GGATACAATTCTTCACCAAT+TGG 0.366004 5.4:-28118768 MS.gene032930:CDS
ATGCAAAACCAAGGCTCAAA+TGG 0.392729 5.4:-28120027 MS.gene032930:CDS
GCATTGTTCATCACAGCTCT+TGG 0.396519 5.4:+28119342 None:intergenic
AAGGCGCAGGAAGCCCTCTC+TGG 0.407977 5.4:-28118789 MS.gene032930:CDS
TTGTTCTTTGTTGTCAGAGT+AGG 0.427163 5.4:+28119627 None:intergenic
GCAGCAGAGAATTCAGTCTT+GGG 0.443647 5.4:-28119321 MS.gene032930:CDS
GATTCTGTTAGATCTGAGAT+TGG 0.449789 5.4:+28118678 None:intergenic
ATGAGAGTGATGGAAGTTCC+AGG 0.452437 5.4:-28119810 MS.gene032930:CDS
TCTGAGTGCATGGCCATTAG+TGG 0.458598 5.4:+28118489 None:intergenic
AAACTATGAGGAGAGGAAAC+AGG 0.459208 5.4:-28118640 MS.gene032930:CDS
TCAGGAGTCCATTTGAGCCT+TGG 0.464509 5.4:+28120019 None:intergenic
TCATGCTCTAGAAATGCAAA+TGG 0.465824 5.4:-28119152 MS.gene032930:CDS
TGCAGCAGAGAATTCAGTCT+TGG 0.471008 5.4:-28119322 MS.gene032930:CDS
ATTGATGCAATTAATCAACT+TGG 0.481050 5.4:-28119980 MS.gene032930:CDS
GATACAATTCTTCACCAATT+GGG 0.481549 5.4:-28118767 MS.gene032930:CDS
ATCTTCTGAAATCTCAGAAA+GGG 0.484544 5.4:-28118577 MS.gene032930:CDS
AGATCTGAGATTGGAGAACT+TGG 0.485427 5.4:+28118687 None:intergenic
CATCTTCTGAAATCTCAGAA+AGG 0.495942 5.4:-28118578 MS.gene032930:CDS
TTTACAATCGGTACTAATGA+AGG 0.497419 5.4:-28118808 MS.gene032930:CDS
AATTCTTCATTAAGGAAAAG+AGG 0.500412 5.4:-28118348 MS.gene032930:CDS
ATTTAAGTAATGAAGTCAAT+GGG 0.515023 5.4:-28118416 MS.gene032930:CDS
AATTTAAGTAATGAAGTCAA+TGG 0.515931 5.4:-28118417 MS.gene032930:CDS
TCAAGGAAAATATTTACAAT+CGG 0.522599 5.4:-28118820 MS.gene032930:CDS
GAAACTTAATGAACAAATTG+AGG 0.524227 5.4:-28119119 MS.gene032930:intron
GATGCAATTAATCAACTTGG+AGG 0.524466 5.4:-28119977 MS.gene032930:CDS
AGATGGTACAAAGTAAGTCC+TGG 0.530608 5.4:+28119792 None:intergenic
TGATTTCTCTTCTGAGTGCA+TGG 0.534501 5.4:+28118479 None:intergenic
CGAAGCACAGACACAGACAT+TGG 0.544995 5.4:+28118447 None:intergenic
CCTGTAAATGGCTCTTGAGA+TGG 0.548306 5.4:+28119775 None:intergenic
AGGGTTAAGTTTAAACTATG+AGG 0.556660 5.4:-28118652 MS.gene032930:CDS
AAAAGATGAACTCTCTCAGT+TGG 0.560309 5.4:-28118733 MS.gene032930:CDS
AAAGAGTATTATGAGAGTGA+TGG 0.568205 5.4:-28119820 MS.gene032930:CDS
CAGCTTCGAATCGAAGCTCA+AGG 0.569311 5.4:-28118837 MS.gene032930:CDS
TAAGTTTAAACTATGAGGAG+AGG 0.577698 5.4:-28118647 MS.gene032930:CDS
ATCGGTACTAATGAAGGCGC+AGG 0.585210 5.4:-28118802 MS.gene032930:CDS
TGAAGAATTGTATCCAGAGA+GGG 0.597554 5.4:+28118776 None:intergenic
GGTTGTAGAAATATAGACTT+GGG 0.601442 5.4:-28119669 MS.gene032930:intron
GTGAAGAATTGTATCCAGAG+AGG 0.605279 5.4:+28118775 None:intergenic
TGGTTGTAGAAATATAGACT+TGG 0.620704 5.4:-28119670 MS.gene032930:intron
TCAGATCTAACAGAATCAAG+AGG 0.622910 5.4:-28118672 MS.gene032930:CDS
TACTCTGACAACAAAGAACA+AGG 0.633344 5.4:-28119625 MS.gene032930:intron
AAATGCAAATGGAAGTAGAG+AGG 0.633743 5.4:-28119141 MS.gene032930:CDS
CAGATCTAACAGAATCAAGA+GGG 0.636389 5.4:-28118671 MS.gene032930:CDS
GAGGAGAGGAAACAGGACAA+TGG 0.639420 5.4:-28118633 MS.gene032930:CDS
CAGCAGAGAATTCAGTCTTG+GGG 0.659289 5.4:-28119320 MS.gene032930:CDS
TCTTCATTAAGGAAAAGAGG+TGG 0.661910 5.4:-28118345 MS.gene032930:CDS
TAGCCAATATCAGAATGACA+AGG 0.715504 5.4:-28118277 MS.gene032930:CDS
TATCCACTGATGCAAAACCA+AGG 0.720964 5.4:-28120036 MS.gene032930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATGAAAAAAAATAGTA+AGG + chr5.4:28118363-28118382 None:intergenic 10.0%
!!! AAAATAATTTTAGATTGAAA+TGG - chr5.4:28119048-28119067 MS.gene032930:intron 10.0%
!! ATTTGAATACATAATGTATT+TGG + chr5.4:28119354-28119373 None:intergenic 15.0%
!!! GTAATGATATTGTAATTTTT+TGG - chr5.4:28118540-28118559 MS.gene032930:CDS 15.0%
!! AAACATTCAATAGAAGAAAT+AGG - chr5.4:28119730-28119749 MS.gene032930:intron 20.0%
!! AAATAGTATGATTGATCATT+TGG - chr5.4:28118874-28118893 MS.gene032930:intron 20.0%
!! AAATGATCAATCATACTATT+TGG + chr5.4:28118875-28118894 None:intergenic 20.0%
!! AATGAAGAAACTAAATTTGA+TGG - chr5.4:28119895-28119914 MS.gene032930:intron 20.0%
!! AATTTAAGTAATGAAGTCAA+TGG - chr5.4:28119862-28119881 MS.gene032930:intron 20.0%
!! ATTTAAGTAATGAAGTCAAT+GGG - chr5.4:28119863-28119882 MS.gene032930:intron 20.0%
!! TACAATATCATTACCAAAAT+GGG + chr5.4:28118534-28118553 None:intergenic 20.0%
!! TCAAGGAAAATATTTACAAT+CGG - chr5.4:28119459-28119478 MS.gene032930:intron 20.0%
!! TTACAATATCATTACCAAAA+TGG + chr5.4:28118535-28118554 None:intergenic 20.0%
!!! ACTATTTTTTGCCAAAATTA+CGG + chr5.4:28119286-28119305 None:intergenic 20.0%
!!! TAAGTACATTTTTTGATTGA+CGG - chr5.4:28119233-28119252 MS.gene032930:intron 20.0%
! AATTCTTCATTAAGGAAAAG+AGG - chr5.4:28119931-28119950 MS.gene032930:intron 25.0%
! ATGTTGAGAATTCTTCATTA+AGG - chr5.4:28119923-28119942 MS.gene032930:intron 25.0%
! ATTGATGCAATTAATCAACT+TGG - chr5.4:28118299-28118318 MS.gene032930:CDS 25.0%
! GAAACTTAATGAACAAATTG+AGG - chr5.4:28119160-28119179 MS.gene032930:CDS 25.0%
! TAATTGCATCAATGAATCTT+TGG + chr5.4:28118292-28118311 None:intergenic 25.0%
! TCAAATTGTGTATGTTAATG+TGG - chr5.4:28118423-28118442 MS.gene032930:CDS 25.0%
!!! GACTTTTCATGTTTTTTTAC+TGG - chr5.4:28118589-28118608 MS.gene032930:CDS 25.0%
AAAATGGGAATACCTGTAAA+TGG + chr5.4:28118519-28118538 None:intergenic 30.0%
AGGGTTAAGTTTAAACTATG+AGG - chr5.4:28119627-28119646 MS.gene032930:CDS 30.0%
ATCACTCTCATAATACTCTT+TGG + chr5.4:28118460-28118479 None:intergenic 30.0%
ATCATTTGGCATAACATTGA+TGG - chr5.4:28118888-28118907 MS.gene032930:intron 30.0%
ATTACTCAGTGATTTGATTC+TGG + chr5.4:28118986-28119005 None:intergenic 30.0%
GATACAATTCTTCACCAATT+GGG - chr5.4:28119512-28119531 MS.gene032930:intron 30.0%
GGTTGTAGAAATATAGACTT+GGG - chr5.4:28118610-28118629 MS.gene032930:CDS 30.0%
TAAGTTTAAACTATGAGGAG+AGG - chr5.4:28119632-28119651 MS.gene032930:CDS 30.0%
TATGCACTAAATGTTGCATA+AGG - chr5.4:28119373-28119392 MS.gene032930:intron 30.0%
TGGTTGTAGAAATATAGACT+TGG - chr5.4:28118609-28118628 MS.gene032930:CDS 30.0%
TTTACAATCGGTACTAATGA+AGG - chr5.4:28119471-28119490 MS.gene032930:intron 30.0%
! ACCGTTAATTTGAAATCAGA+CGG + chr5.4:28118810-28118829 None:intergenic 30.0%
! ATTTACAGGTATTCCCATTT+TGG - chr5.4:28118518-28118537 MS.gene032930:CDS 30.0%
!! AAAGAGTATTATGAGAGTGA+TGG - chr5.4:28118459-28118478 MS.gene032930:CDS 30.0%
!! ATCTTCTGAAATCTCAGAAA+GGG - chr5.4:28119702-28119721 MS.gene032930:intron 30.0%
!! TCAATTTGGATGTTGATTTC+AGG - chr5.4:28119405-28119424 MS.gene032930:intron 30.0%
!! TTTGTGAGTTTTATCCCAAT+TGG + chr5.4:28119529-28119548 None:intergenic 30.0%
!!! TTTTGTTTTTGCCAGAAACA+TGG - chr5.4:28119097-28119116 MS.gene032930:intron 30.0%
AAAAGATGAACTCTCTCAGT+TGG - chr5.4:28119546-28119565 MS.gene032930:intron 35.0%
AAATGCAAATGGAAGTAGAG+AGG - chr5.4:28119138-28119157 MS.gene032930:CDS 35.0%
ACCATCTCGTATCAAAATAG+TGG - chr5.4:28118737-28118756 MS.gene032930:CDS 35.0%
AGTCCTTGTCATTCTGATAT+TGG + chr5.4:28120008-28120027 None:intergenic 35.0%
ATGAATCTTTGGTGAAGTTC+AGG + chr5.4:28118281-28118300 None:intergenic 35.0%
ATGAGATATCTCCATGTTTC+TGG + chr5.4:28119111-28119130 None:intergenic 35.0%
CAGATCTAACAGAATCAAGA+GGG - chr5.4:28119608-28119627 MS.gene032930:intron 35.0%
GATGCAATTAATCAACTTGG+AGG - chr5.4:28118302-28118321 MS.gene032930:CDS 35.0%
GCCGTCTGATTTCAAATTAA+CGG - chr5.4:28118806-28118825 MS.gene032930:CDS 35.0%
GGATACAATTCTTCACCAAT+TGG - chr5.4:28119511-28119530 MS.gene032930:intron 35.0%
TACTCTGACAACAAAGAACA+AGG - chr5.4:28118654-28118673 MS.gene032930:CDS 35.0%
TAGCCAATATCAGAATGACA+AGG - chr5.4:28120002-28120021 MS.gene032930:CDS 35.0%
TCAGATCTAACAGAATCAAG+AGG - chr5.4:28119607-28119626 MS.gene032930:intron 35.0%
TCTTCATTAAGGAAAAGAGG+TGG - chr5.4:28119934-28119953 MS.gene032930:intron 35.0%
TGAAGAATTGTATCCAGAGA+GGG + chr5.4:28119506-28119525 None:intergenic 35.0%
TGGAACTTCTTTGTGTTCAT+TGG - chr5.4:28119666-28119685 MS.gene032930:intron 35.0%
TTCACTTGAATTGCCACTAA+TGG - chr5.4:28119777-28119796 MS.gene032930:CDS 35.0%
! AGAACAAGGTAATGACTTGT+AGG - chr5.4:28118668-28118687 MS.gene032930:CDS 35.0%
! GAACAAGGTAATGACTTGTA+GGG - chr5.4:28118669-28118688 MS.gene032930:CDS 35.0%
! GATTCTGTTAGATCTGAGAT+TGG + chr5.4:28119604-28119623 None:intergenic 35.0%
! TAAGGACGTGTTGATCAATT+TGG - chr5.4:28119391-28119410 MS.gene032930:intron 35.0%
! TGTATTTGGTGAAATCACAG+TGG + chr5.4:28119340-28119359 None:intergenic 35.0%
! TTGTTCTTTGTTGTCAGAGT+AGG + chr5.4:28118655-28118674 None:intergenic 35.0%
!! CATAACATTGATGGTCCTTT+TGG - chr5.4:28118897-28118916 MS.gene032930:intron 35.0%
!! CATCTTCTGAAATCTCAGAA+AGG - chr5.4:28119701-28119720 MS.gene032930:intron 35.0%
!! TCATGCTCTAGAAATGCAAA+TGG - chr5.4:28119127-28119146 MS.gene032930:CDS 35.0%
!! TTTTTGATTGACGGTGAGTT+TGG - chr5.4:28119242-28119261 MS.gene032930:intron 35.0%
!!! ACCACTATTTTGATACGAGA+TGG + chr5.4:28118741-28118760 None:intergenic 35.0%
AAACTATGAGGAGAGGAAAC+AGG - chr5.4:28119639-28119658 MS.gene032930:CDS 40.0%
AGATGGTACAAAGTAAGTCC+TGG + chr5.4:28118490-28118509 None:intergenic 40.0%
ATGCAAAACCAAGGCTCAAA+TGG - chr5.4:28118252-28118271 MS.gene032930:CDS 40.0%
GTGAAGAATTGTATCCAGAG+AGG + chr5.4:28119507-28119526 None:intergenic 40.0%
TATCCACTGATGCAAAACCA+AGG - chr5.4:28118243-28118262 MS.gene032930:CDS 40.0%
TGATTTCTCTTCTGAGTGCA+TGG + chr5.4:28119803-28119822 None:intergenic 40.0%
! AGATCTGAGATTGGAGAACT+TGG + chr5.4:28119595-28119614 None:intergenic 40.0%
! ATGGTGTGCTACCGTAATTT+TGG - chr5.4:28119272-28119291 MS.gene032930:intron 40.0%
!! CAGAGTAGGTTTCTAGTTGT+TGG + chr5.4:28118641-28118660 None:intergenic 40.0%
CAGCAGAGAATTCAGTCTTG+GGG - chr5.4:28118959-28118978 MS.gene032930:intron 45.0%
CCATCTCAAGAGCCATTTAC+AGG - chr5.4:28118504-28118523 MS.gene032930:CDS 45.0%
CCTGTAAATGGCTCTTGAGA+TGG + chr5.4:28118507-28118526 None:intergenic 45.0%
CGGTGAGTTTGGTAGAATCA+TGG - chr5.4:28119253-28119272 MS.gene032930:intron 45.0%
GCAGCAGAGAATTCAGTCTT+GGG - chr5.4:28118958-28118977 MS.gene032930:intron 45.0%
GCATTGTTCATCACAGCTCT+TGG + chr5.4:28118940-28118959 None:intergenic 45.0%
TCTGTGTACACTGCACCAAA+AGG + chr5.4:28118915-28118934 None:intergenic 45.0%
TGCAGCAGAGAATTCAGTCT+TGG - chr5.4:28118957-28118976 MS.gene032930:intron 45.0%
! ATGAGAGTGATGGAAGTTCC+AGG - chr5.4:28118469-28118488 MS.gene032930:CDS 45.0%
CAGCTTCGAATCGAAGCTCA+AGG - chr5.4:28119442-28119461 MS.gene032930:intron 50.0%
CGAAGCACAGACACAGACAT+TGG + chr5.4:28119835-28119854 None:intergenic 50.0%
GAGGAGAGGAAACAGGACAA+TGG - chr5.4:28119646-28119665 MS.gene032930:CDS 50.0%
TCAGGAGTCCATTTGAGCCT+TGG + chr5.4:28118263-28118282 None:intergenic 50.0%
TCTGAGTGCATGGCCATTAG+TGG + chr5.4:28119793-28119812 None:intergenic 50.0%
! ATCGGTACTAATGAAGGCGC+AGG - chr5.4:28119477-28119496 MS.gene032930:intron 50.0%
!!! GAGCCTTGGTTTTGCATCAG+TGG + chr5.4:28118249-28118268 None:intergenic 50.0%
AAGGCGCAGGAAGCCCTCTC+TGG - chr5.4:28119490-28119509 MS.gene032930:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 28118215 28120086 28118215 ID=MS.gene032930
chr5.4 mRNA 28118215 28120086 28118215 ID=MS.gene032930.t1;Parent=MS.gene032930
chr5.4 exon 28119969 28120086 28119969 ID=MS.gene032930.t1.exon1;Parent=MS.gene032930.t1
chr5.4 CDS 28119969 28120086 28119969 ID=cds.MS.gene032930.t1;Parent=MS.gene032930.t1
chr5.4 exon 28119776 28119852 28119776 ID=MS.gene032930.t1.exon2;Parent=MS.gene032930.t1
chr5.4 CDS 28119776 28119852 28119776 ID=cds.MS.gene032930.t1;Parent=MS.gene032930.t1
chr5.4 exon 28119626 28119683 28119626 ID=MS.gene032930.t1.exon3;Parent=MS.gene032930.t1
chr5.4 CDS 28119626 28119683 28119626 ID=cds.MS.gene032930.t1;Parent=MS.gene032930.t1
chr5.4 exon 28119301 28119376 28119301 ID=MS.gene032930.t1.exon4;Parent=MS.gene032930.t1
chr5.4 CDS 28119301 28119376 28119301 ID=cds.MS.gene032930.t1;Parent=MS.gene032930.t1
chr5.4 exon 28119120 28119189 28119120 ID=MS.gene032930.t1.exon5;Parent=MS.gene032930.t1
chr5.4 CDS 28119120 28119189 28119120 ID=cds.MS.gene032930.t1;Parent=MS.gene032930.t1
chr5.4 exon 28118215 28118874 28118215 ID=MS.gene032930.t1.exon6;Parent=MS.gene032930.t1
chr5.4 CDS 28118215 28118874 28118215 ID=cds.MS.gene032930.t1;Parent=MS.gene032930.t1
Gene Sequence

>MS.gene032930

ATGCAGAATCAAAGTATGCATTTTGTTCTATCCACTGATGCAAAACCAAGGCTCAAATGGACTCCTGAACTTCACCAAAGATTCATTGATGCAATTAATCAACTTGGAGGTGCAGCTAGTAAGTACAAAACAAACTCAATAATGACCTTACTATTTTTTTTCATATTTTTATTACACTTTAGAGCATAAATGTGACTTAATGTATCATTCAAATTGTGTATGTTAATGTGGCAGAAGCAACACCAAAGAGTATTATGAGAGTGATGGAAGTTCCAGGACTTACTTTGTACCATCTCAAGAGCCATTTACAGGTATTCCCATTTTGGTAATGATATTGTAATTTTTTGGTTATTTTTGTTGACTGAAGAAGTTGTGACTTTTCATGTTTTTTTACTGGTTGTAGAAATATAGACTTGGGAAAAGCCAACAACTAGAAACCTACTCTGACAACAAAGAACAAGGTAATGACTTGTAGGGAAACATATTTACTTCAGTAACTGTGTCGCACAGTTTTCAATCTCGACCATCTCGTATCAAAATAGTGGTCGAAATTGTTTATAGACTAATTGATTTTGTTATTGTGTAATTTGAGCCGTCTGATTTCAAATTAACGGTTAAGATTGTTTACAACGTGTAAATGTGTGTGTGTCTGTTAATCCAAATAGTATGATTGATCATTTGGCATAACATTGATGGTCCTTTTGGTGCAGTGTACACAGAAACCAAGAGCTGTGATGAACAATGCAGCAGAGAATTCAGTCTTGGGGACCAGAATCAAATCACTGAGTAATAATTAGAGCTTTTATCAAATATTTGATTATGATTTTTTCGACAAAATAATTTTAGATTGAAATGGTTTTAGCTAGAGATTTTATTTTCAAATTTTGTTTTTGCCAGAAACATGGAGATATCTCATGCTCTAGAAATGCAAATGGAAGTAGAGAGGAAACTTAATGAACAAATTGAGGTAACATGAATAATGTGACATCATGATTAAAATTTGCTTCAATCATAATTCTAAGTACATTTTTTGATTGACGGTGAGTTTGGTAGAATCATGGTGTGCTACCGTAATTTTGGCAAAAAATAGTACACTTTTTAAGCTGCAACAAAATTACGTAGCCACTGTGATTTCACCAAATACATTATGTATTCAAATATGCACTAAATGTTGCATAAGGACGTGTTGATCAATTTGGATGTTGATTTCAGGTGCAGAAACATTTGCAGCTTCGAATCGAAGCTCAAGGAAAATATTTACAATCGGTACTAATGAAGGCGCAGGAAGCCCTCTCTGGATACAATTCTTCACCAATTGGGATAAAACTCACAAAAGATGAACTCTCTCAGTTGGTTACTATGATCAACAATGCATGTCCAAGTTCTCCAATCTCAGATCTAACAGAATCAAGAGGGTTAAGTTTAAACTATGAGGAGAGGAAACAGGACAATGGAACTTCTTTGTGTTCATTGGAAAGTTCTTTGACATCTTCTGAAATCTCAGAAAGGGAAGATAAACATTCAATAGAAGAAATAGGAGACTTCAAAAATTCTAGTGCTATTTCACTTGAATTGCCACTAATGGCCATGCACTCAGAAGAGAAATCATTGTCGTGTCCAATGTCTGTGTCTGTGCTTCGTAGCGATAATTTAAGTAATGAAGTCAATGGGAGAAAGAGAAATGAAGAAACTAAATTTGATGGTAGCTATGTTGAGAATTCTTCATTAAGGAAAAGAGGTGGCAACAAGTTGAAGAAAGCTAAGTTGTCAGAAAAGTTTGACTTGAATAGCCAATATCAGAATGACAAGGACTCAACAAGTTCAAAAACGTTACTAGACTTAAATTGCAGTTTAAACTTTTGTGAACCATGA
Protein sequence

>MS.gene032930.t1

MQNQSMHFVLSTDAKPRLKWTPELHQRFIDAINQLGGAAKATPKSIMRVMEVPGLTLYHLKSHLQKYRLGKSQQLETYSDNKEQVYTETKSCDEQCSREFSLGDQNQITENMEISHALEMQMEVERKLNEQIEVQKHLQLRIEAQGKYLQSVLMKAQEALSGYNSSPIGIKLTKDELSQLVTMINNACPSSPISDLTESRGLSLNYEERKQDNGTSLCSLESSLTSSEISEREDKHSIEEIGDFKNSSAISLELPLMAMHSEEKSLSCPMSVSVLRSDNLSNEVNGRKRNEETKFDGSYVENSSLRKRGGNKLKKAKLSEKFDLNSQYQNDKDSTSSKTLLDLNCSLNFCEP