AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032932

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032932.t1 MTR_5g041380 85.901 383 38 2 1 379 1 371 0.0 642
MS.gene032932.t1 MTR_5g041400 84.433 379 43 2 1 379 1 363 0.0 630
MS.gene032932.t1 MTR_5g041530 64.736 397 106 11 1 379 1 381 3.25e-151 432
MS.gene032932.t1 MTR_5g041420 62.468 389 69 10 1 379 1 322 5.72e-130 375
MS.gene032932.t1 MTR_4g082060 38.776 490 154 20 4 378 6 464 7.27e-75 239
MS.gene032932.t1 MTR_7g010950 33.803 497 192 17 2 377 4 484 1.14e-64 214
MS.gene032932.t1 MTR_6g012450 33.137 510 183 20 4 377 6 493 3.68e-54 186
MS.gene032932.t1 MTR_3g435480 36.508 378 128 14 101 377 97 463 4.77e-52 180
MS.gene032932.t1 MTR_8g044220 29.806 463 203 19 4 379 12 439 1.31e-40 149
MS.gene032932.t1 MTR_6g027460 58.586 99 35 2 96 188 51 149 4.89e-32 124
MS.gene032932.t1 MTR_7g086780 29.700 367 154 12 103 379 70 422 2.73e-30 120
MS.gene032932.t1 MTR_2g016030 70.769 65 19 0 124 188 36 100 8.67e-30 112
MS.gene032932.t1 MTR_6g027450 54.167 96 38 2 99 188 47 142 1.02e-27 111
MS.gene032932.t1 MTR_8g079060 63.492 63 23 0 135 197 29 91 5.32e-23 96.7
MS.gene032932.t1 MTR_4g022370 51.429 70 34 0 119 188 66 135 1.16e-21 95.5
MS.gene032932.t1 MTR_8g027295 56.250 64 28 0 125 188 14 77 2.98e-21 92.8
MS.gene032932.t1 MTR_2g093220 57.353 68 29 0 121 188 26 93 5.04e-21 92.4
MS.gene032932.t1 MTR_3g091820 49.398 83 38 1 109 191 21 99 6.38e-21 92.4
MS.gene032932.t1 MTR_2g096740 67.308 52 17 0 137 188 79 130 7.01e-21 92.4
MS.gene032932.t1 MTR_8g479350 69.231 52 16 0 137 188 70 121 7.36e-21 92.8
MS.gene032932.t1 MTR_2g096740 67.308 52 17 0 137 188 64 115 7.40e-21 92.0
MS.gene032932.t1 MTR_4g089095 64.151 53 19 0 137 189 52 104 1.09e-20 91.7
MS.gene032932.t1 MTR_4g089095 64.151 53 19 0 137 189 18 70 1.29e-20 90.9
MS.gene032932.t1 MTR_4g088580 71.154 52 15 0 137 188 57 108 1.38e-20 92.8
MS.gene032932.t1 MTR_4g109980 64.151 53 19 0 133 185 38 90 1.83e-20 91.3
MS.gene032932.t1 MTR_1g077600 64.151 53 19 0 135 187 43 95 1.86e-20 90.5
MS.gene032932.t1 MTR_8g015840 67.308 52 17 0 135 186 20 71 1.87e-20 89.4
MS.gene032932.t1 MTR_4g461080 61.538 65 21 1 127 187 17 81 2.10e-20 90.5
MS.gene032932.t1 MTR_1g077600 64.151 53 19 0 135 187 43 95 2.24e-20 90.5
MS.gene032932.t1 MTR_7g024670 68.627 51 16 0 137 187 79 129 2.33e-20 91.7
MS.gene032932.t1 MTR_1g056810 52.941 85 37 3 125 208 1 83 2.95e-20 90.1
MS.gene032932.t1 MTR_7g059400 51.429 70 34 0 119 188 60 129 3.62e-20 90.9
MS.gene032932.t1 MTR_4g063780 43.269 104 58 1 119 221 59 162 4.15e-20 90.5
MS.gene032932.t1 MTR_2g013370 68.519 54 16 1 135 188 46 98 4.34e-20 89.4
MS.gene032932.t1 MTR_8g068210 45.882 85 38 1 104 188 9 85 4.40e-20 90.5
MS.gene032932.t1 MTR_3g090430 63.158 57 20 1 133 188 48 104 1.11e-19 89.4
MS.gene032932.t1 MTR_2g059540 67.347 49 16 0 135 183 31 79 1.30e-19 86.7
MS.gene032932.t1 MTR_2g014170 67.347 49 16 0 135 183 45 93 1.09e-18 86.3
MS.gene032932.t1 MTR_5g031440 60.000 55 22 0 131 185 39 93 1.10e-18 86.7
MS.gene032932.t1 MTR_2g014060 66.000 50 17 0 137 186 27 76 1.58e-18 85.9
MS.gene032932.t1 MTR_3g077750 64.706 51 18 0 135 185 61 111 4.70e-18 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032932.t1 AT5G62430 45.675 289 127 9 106 379 25 298 3.59e-63 204
MS.gene032932.t1 AT3G47500 34.091 484 173 18 2 379 5 448 1.27e-54 186
MS.gene032932.t1 AT5G39660 32.829 463 212 16 4 377 3 455 2.62e-49 172
MS.gene032932.t1 AT5G39660 32.829 463 212 16 4 377 3 455 2.62e-49 172
MS.gene032932.t1 AT5G39660 32.829 463 212 16 4 377 3 455 2.62e-49 172
MS.gene032932.t1 AT1G69570 33.810 420 210 20 2 379 4 397 1.56e-44 158
MS.gene032932.t1 AT1G26790 34.062 320 170 11 90 379 64 372 1.00e-40 147
MS.gene032932.t1 AT1G29160 71.212 66 19 0 123 188 50 115 5.04e-32 119
MS.gene032932.t1 AT2G34140 55.914 93 36 2 101 188 19 111 1.55e-30 115
MS.gene032932.t1 AT3G55370 35.075 134 76 1 57 190 9 131 4.88e-23 98.6
MS.gene032932.t1 AT3G55370 35.075 134 76 1 57 190 9 131 8.16e-23 98.6
MS.gene032932.t1 AT1G51700 51.190 84 31 1 111 194 19 92 4.83e-22 93.2
MS.gene032932.t1 AT5G60200 57.143 70 30 0 125 194 45 114 6.31e-22 94.4
MS.gene032932.t1 AT3G55370 47.126 87 45 1 105 190 90 176 9.20e-22 95.9
MS.gene032932.t1 AT3G50410 71.154 52 15 0 135 186 30 81 1.14e-21 93.6
MS.gene032932.t1 AT3G21270 72.549 51 14 0 137 187 31 81 2.95e-21 91.3
MS.gene032932.t1 AT3G61850 42.157 102 59 0 90 191 17 118 3.44e-21 92.8
MS.gene032932.t1 AT3G61850 42.157 102 59 0 90 191 17 118 3.44e-21 92.8
MS.gene032932.t1 AT3G61850 42.157 102 59 0 90 191 29 130 3.88e-21 92.8
MS.gene032932.t1 AT3G61850 42.157 102 59 0 90 191 17 118 4.98e-21 92.8
MS.gene032932.t1 AT3G61850 42.157 102 59 0 90 191 29 130 5.23e-21 93.2
MS.gene032932.t1 AT2G28510 67.241 58 19 0 137 194 50 107 7.38e-21 92.0
MS.gene032932.t1 AT5G02460 52.857 70 33 0 119 188 79 148 8.67e-21 93.6
MS.gene032932.t1 AT5G60850 66.071 56 19 0 135 190 51 106 1.06e-20 92.0
MS.gene032932.t1 AT5G65590 49.425 87 41 1 104 190 13 96 1.23e-20 92.0
MS.gene032932.t1 AT5G66940 70.588 51 15 0 137 187 34 84 1.76e-20 89.7
MS.gene032932.t1 AT1G07640 68.627 51 16 0 137 187 23 73 3.15e-20 90.1
MS.gene032932.t1 AT1G28310 66.667 54 18 0 135 188 27 80 3.23e-20 90.5
MS.gene032932.t1 AT2G28810 52.239 67 31 1 121 186 62 128 3.31e-20 90.9
MS.gene032932.t1 AT4G24060 63.158 57 21 0 132 188 50 106 3.44e-20 90.9
MS.gene032932.t1 AT3G45610 59.016 61 25 0 131 191 36 96 3.54e-20 89.4
MS.gene032932.t1 AT1G07640 68.627 51 16 0 137 187 79 129 3.85e-20 90.9
MS.gene032932.t1 AT1G28310 66.667 54 18 0 135 188 51 104 4.24e-20 90.5
MS.gene032932.t1 AT1G07640 68.627 51 16 0 137 187 87 137 4.49e-20 90.5
MS.gene032932.t1 AT2G37590 58.621 58 24 0 131 188 85 142 4.52e-20 90.5
MS.gene032932.t1 AT2G28810 52.239 67 31 1 121 186 79 145 4.63e-20 90.5
MS.gene032932.t1 AT1G47655 69.388 49 15 0 135 183 29 77 1.27e-19 86.7
MS.gene032932.t1 AT4G00940 64.286 56 20 0 137 192 70 125 4.00e-19 87.0
MS.gene032932.t1 AT4G00940 64.286 56 20 0 137 192 70 125 4.00e-19 87.0
MS.gene032932.t1 AT4G00940 64.286 56 20 0 137 192 70 125 4.00e-19 87.0
MS.gene032932.t1 AT4G38000 56.250 64 27 1 130 192 36 99 4.55e-19 86.3
MS.gene032932.t1 AT1G64620 51.471 68 29 1 116 183 34 97 4.99e-19 87.8
MS.gene032932.t1 AT1G21340 49.398 83 39 1 118 200 21 100 5.26e-19 86.3
MS.gene032932.t1 AT3G52440 61.404 57 21 1 133 188 22 78 7.65e-19 85.5
MS.gene032932.t1 AT3G52440 61.404 57 21 1 133 188 41 97 1.00e-18 85.5
MS.gene032932.t1 AT5G62940 67.347 49 16 0 137 185 75 123 5.22e-18 85.1
MS.gene032932.t1 AT2G46590 59.615 52 21 0 131 182 64 115 2.17e-17 83.2
MS.gene032932.t1 AT2G46590 59.615 52 21 0 131 182 76 127 2.37e-17 83.2
MS.gene032932.t1 AT4G21050 50.725 69 32 1 120 188 11 77 3.23e-17 80.1
MS.gene032932.t1 AT4G21040 45.679 81 40 2 137 216 27 104 2.93e-15 75.1
MS.gene032932.t1 AT4G21080 55.769 52 23 0 137 188 27 78 1.35e-14 73.6

Find 71 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCTTCCGAAAAGCTTTA+TGG 0.176107 5.4:-28168204 None:intergenic
TTTCAATGGATTTGCATCTA+TGG 0.224783 5.4:+28169610 MS.gene032932:CDS
GTTCCAAGTAAACCTACTTT+AGG 0.234419 5.4:+28169437 MS.gene032932:CDS
AAACCCTTTGCATCTTCATT+AGG 0.263138 5.4:-28169548 None:intergenic
TCTTCAATTCTCAATTTCTT+AGG 0.264262 5.4:-28169524 None:intergenic
GCAAAAGCTGCCAGAGATAT+TGG 0.290195 5.4:+28169075 MS.gene032932:CDS
ACCACCATAGTATGCTATAT+TGG 0.313954 5.4:-28169366 None:intergenic
AACCCTTTGCATCTTCATTA+GGG 0.318049 5.4:-28169547 None:intergenic
CTTGTCAAGAAAATCAATAT+TGG 0.352841 5.4:+28169248 MS.gene032932:CDS
TGCCAGAGATATTGGACTTC+CGG 0.368326 5.4:+28169083 MS.gene032932:CDS
TTCCAAGTAAACCTACTTTA+GGG 0.369270 5.4:+28169438 MS.gene032932:CDS
ATGAGTAGTGTCTTGACCTT+TGG 0.369998 5.4:+28169194 MS.gene032932:CDS
TTCCCTAAAGTAGGTTTACT+TGG 0.378626 5.4:-28169440 None:intergenic
AGTGAAGATGAAGACATAAT+AGG 0.382456 5.4:-28169227 None:intergenic
ACATGATTCAAAGAGATATA+AGG 0.401349 5.4:+28168361 MS.gene032932:CDS
CAACCCAATATAGCATACTA+TGG 0.408858 5.4:+28169362 MS.gene032932:CDS
CCACCATAGTATGCTATATT+GGG 0.415830 5.4:-28169365 None:intergenic
ATCAATTTCAGGAACAATCA+AGG 0.419402 5.4:+28168775 MS.gene032932:intron
CCTTGATAAAGCTGCAGGAT+TGG 0.440224 5.4:-28169681 None:intergenic
GACACAGGATAACACATTGC+TGG 0.441391 5.4:-28169335 None:intergenic
TTCAATGGATTTGCATCTAT+GGG 0.443267 5.4:+28169611 MS.gene032932:CDS
TTGCATCTATGGGTGGTGAT+AGG 0.444216 5.4:+28169621 MS.gene032932:CDS
CTTGAATGTTTCCCTAAAGT+AGG 0.465584 5.4:-28169449 None:intergenic
TTTGAAGAATTAACTTCAAG+TGG 0.465849 5.4:-28168335 None:intergenic
CTCCGGAAGTCCAATATCTC+TGG 0.468706 5.4:-28169085 None:intergenic
GCAGAAGTGACGGGGCTGGT+TGG 0.486295 5.4:-28169054 None:intergenic
GAAGTTTCTGTTTCTGATGA+GGG 0.487694 5.4:-28168903 None:intergenic
TAACACATTGCTGGGTTCCA+TGG 0.491823 5.4:-28169326 None:intergenic
CTCAATAAGGCTTGAAGATG+TGG 0.492620 5.4:-28168868 None:intergenic
GTGGTTGTTTAGTCCCATCA+TGG 0.499829 5.4:+28169384 MS.gene032932:CDS
TTCCCAATCAAATCCTCTCA+TGG 0.512251 5.4:-28169715 None:intergenic
CATACTTCTAGTGGTTCCTC+CGG 0.515524 5.4:-28169102 None:intergenic
AGAAGTTTCTGTTTCTGATG+AGG 0.515525 5.4:-28168904 None:intergenic
CCAATCCTGCAGCTTTATCA+AGG 0.521791 5.4:+28169681 MS.gene032932:CDS
TTCATAATCAAGTACTGAGG+TGG 0.527250 5.4:-28168805 None:intergenic
AGAAGTATGCTTGTAGGTGC+AGG 0.531689 5.4:+28169116 MS.gene032932:CDS
TTAGGGAAACATTCAAGAGA+TGG 0.534355 5.4:+28169455 MS.gene032932:CDS
ACCACTAGAAGTATGCTTGT+AGG 0.541265 5.4:+28169110 MS.gene032932:CDS
TAGGTTGTACGCTCCATGAT+GGG 0.544678 5.4:-28169397 None:intergenic
TGAGACCTTGATAAAGCTGC+AGG 0.554542 5.4:-28169686 None:intergenic
CATAGTATGCTATATTGGGT+TGG 0.557895 5.4:-28169361 None:intergenic
ACACAGGATAACACATTGCT+GGG 0.569497 5.4:-28169334 None:intergenic
GAGAAATCTTGATGAGCAAG+AGG 0.571155 5.4:-28168275 None:intergenic
TCATAATCAAGTACTGAGGT+GGG 0.572134 5.4:-28168804 None:intergenic
GACCCTAATGAAGATGCAAA+GGG 0.573223 5.4:+28169545 MS.gene032932:CDS
CTAGGAAACAAGAGTGGTGG+AGG 0.573390 5.4:+28169584 MS.gene032932:CDS
TTTGTTAGGAGCTTTGTCCT+GGG 0.573883 5.4:-28168967 None:intergenic
ATAGGTTGTACGCTCCATGA+TGG 0.581466 5.4:-28169398 None:intergenic
ATAAGGCTTGAAGATGTGGT+AGG 0.588303 5.4:-28168864 None:intergenic
GGGTTCCATGGAAACTGTTG+TGG 0.589831 5.4:-28169314 None:intergenic
AGACCCTAATGAAGATGCAA+AGG 0.590368 5.4:+28169544 MS.gene032932:CDS
GGTCTCATGTGTTCCATGAG+AGG 0.592053 5.4:+28169702 MS.gene032932:CDS
ACACAAGATTGAGTTGCGAT+AGG 0.596525 5.4:-28169416 None:intergenic
AATAGGAGAATCTGATCCAA+AGG 0.598047 5.4:-28169210 None:intergenic
TTGACGATTATGTGAGACAT+CGG 0.603312 5.4:-28169171 None:intergenic
AGGAGTGTTTAAATCCTCTG+TGG 0.606748 5.4:-28168844 None:intergenic
ATTGGCTTTCAATACTGATG+AGG 0.608635 5.4:-28169663 None:intergenic
AAACTCTGCGGAATAATCAT+CGG 0.614237 5.4:-28168246 None:intergenic
TGAACAAAGTGATATGTCCC+AGG 0.620522 5.4:+28168950 MS.gene032932:CDS
AATGGATTTGCATCTATGGG+TGG 0.631829 5.4:+28169614 MS.gene032932:CDS
ACCTACAAGCATACTTCTAG+TGG 0.641003 5.4:-28169111 None:intergenic
CCCAATATAGCATACTATGG+TGG 0.653975 5.4:+28169365 MS.gene032932:CDS
ACACTAGGAAACAAGAGTGG+TGG 0.656394 5.4:+28169581 MS.gene032932:CDS
AAATCTTGATGAGCAAGAGG+AGG 0.659290 5.4:-28168272 None:intergenic
ATATTGGGTTGGAATGACAC+AGG 0.664359 5.4:-28169350 None:intergenic
CAGAGATATTGGACTTCCGG+AGG 0.693631 5.4:+28169086 MS.gene032932:CDS
TTCTTCATAATCAAGTACTG+AGG 0.698198 5.4:-28168808 None:intergenic
GGGTGGTGATAGGAATCATG+TGG 0.704890 5.4:+28169631 MS.gene032932:CDS
TTAACACTAGGAAACAAGAG+TGG 0.767808 5.4:+28169578 MS.gene032932:CDS
AAGAGGAGGAGAAAACTCTG+CGG 0.769613 5.4:-28168258 None:intergenic
AGCCTCAAAACAAACCACAG+AGG 0.778500 5.4:+28168830 MS.gene032932:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAATTACTTATAGTT+AGG + chr5.4:28168420-28168439 MS.gene032932:intron 10.0%
!! AATTATTGTTTATCAATTTC+AGG + chr5.4:28168764-28168783 MS.gene032932:intron 15.0%
!! TGATAAACAATAATTAGATT+AGG - chr5.4:28168759-28168778 None:intergenic 15.0%
!! ATAGACTTCAAAATATGAAT+TGG - chr5.4:28168676-28168695 None:intergenic 20.0%
!!! AAGAAGAGATTTTATTCTTA+CGG - chr5.4:28169145-28169164 None:intergenic 20.0%
!!! TGAAAAATATACCAAGTTTT+TGG + chr5.4:28168595-28168614 MS.gene032932:intron 20.0%
!!! TTTTGGTGATAAAATTCTTA+TGG + chr5.4:28168549-28168568 MS.gene032932:intron 20.0%
! ACATGATTCAAAGAGATATA+AGG + chr5.4:28168361-28168380 MS.gene032932:CDS 25.0%
! CTTGTCAAGAAAATCAATAT+TGG + chr5.4:28169248-28169267 MS.gene032932:CDS 25.0%
! TCTTCAATTCTCAATTTCTT+AGG - chr5.4:28169527-28169546 None:intergenic 25.0%
! TTTGAAGAATTAACTTCAAG+TGG - chr5.4:28168338-28168357 None:intergenic 25.0%
!! ACTTTGTTCATCAATTTTAG+TGG - chr5.4:28168940-28168959 None:intergenic 25.0%
AGTGAAGATGAAGACATAAT+AGG - chr5.4:28169230-28169249 None:intergenic 30.0%
ATCAATTTCAGGAACAATCA+AGG + chr5.4:28168775-28168794 MS.gene032932:intron 30.0%
CATCATGAAATCCAAAAACT+TGG - chr5.4:28168609-28168628 None:intergenic 30.0%
TAACTTTGTGGTTCATATCT+TGG + chr5.4:28168728-28168747 MS.gene032932:intron 30.0%
TGAAGTCTATAATCCTAAAC+TGG + chr5.4:28168686-28168705 MS.gene032932:intron 30.0%
TTCCAAGTAAACCTACTTTA+GGG + chr5.4:28169438-28169457 MS.gene032932:CDS 30.0%
! GATTCAACCATAAAGCTTTT+CGG + chr5.4:28168197-28168216 MS.gene032932:CDS 30.0%
! TTCAATGGATTTGCATCTAT+GGG + chr5.4:28169611-28169630 MS.gene032932:CDS 30.0%
! TTCTTCATAATCAAGTACTG+AGG - chr5.4:28168811-28168830 None:intergenic 30.0%
! TTTCAATGGATTTGCATCTA+TGG + chr5.4:28169610-28169629 MS.gene032932:CDS 30.0%
!! AAGATGCAAAGGGTTTTAAT+TGG + chr5.4:28169555-28169574 MS.gene032932:CDS 30.0%
!!! GGTTTTAATTGGTTAACACT+AGG + chr5.4:28169566-28169585 MS.gene032932:CDS 30.0%
!!! GTGCACTTTTTTCATCTTTT+TGG + chr5.4:28168532-28168551 MS.gene032932:intron 30.0%
AAACCCTTTGCATCTTCATT+AGG - chr5.4:28169551-28169570 None:intergenic 35.0%
AAACTCTGCGGAATAATCAT+CGG - chr5.4:28168249-28168268 None:intergenic 35.0%
AACCCTTTGCATCTTCATTA+GGG - chr5.4:28169550-28169569 None:intergenic 35.0%
AATAGGAGAATCTGATCCAA+AGG - chr5.4:28169213-28169232 None:intergenic 35.0%
ACCACCATAGTATGCTATAT+TGG - chr5.4:28169369-28169388 None:intergenic 35.0%
AGTTGCAATCAAACCAGTTT+AGG - chr5.4:28168702-28168721 None:intergenic 35.0%
CAACCCAATATAGCATACTA+TGG + chr5.4:28169362-28169381 MS.gene032932:CDS 35.0%
CATAGTATGCTATATTGGGT+TGG - chr5.4:28169364-28169383 None:intergenic 35.0%
CCACCATAGTATGCTATATT+GGG - chr5.4:28169368-28169387 None:intergenic 35.0%
CTTGAATGTTTCCCTAAAGT+AGG - chr5.4:28169452-28169471 None:intergenic 35.0%
GCAACTTCAACTTAACTTTG+TGG + chr5.4:28168716-28168735 MS.gene032932:intron 35.0%
GTTCCAAGTAAACCTACTTT+AGG + chr5.4:28169437-28169456 MS.gene032932:CDS 35.0%
TTAACACTAGGAAACAAGAG+TGG + chr5.4:28169578-28169597 MS.gene032932:CDS 35.0%
TTAGGGAAACATTCAAGAGA+TGG + chr5.4:28169455-28169474 MS.gene032932:CDS 35.0%
TTCCCTAAAGTAGGTTTACT+TGG - chr5.4:28169443-28169462 None:intergenic 35.0%
TTGACGATTATGTGAGACAT+CGG - chr5.4:28169174-28169193 None:intergenic 35.0%
! GGACATGGAACAATTTTGTT+AGG - chr5.4:28168984-28169003 None:intergenic 35.0%
! TTTTTCCACAACAGTTTCCA+TGG + chr5.4:28169309-28169328 MS.gene032932:CDS 35.0%
!! AGAAGTTTCTGTTTCTGATG+AGG - chr5.4:28168907-28168926 None:intergenic 35.0%
!! ATTGGCTTTCAATACTGATG+AGG - chr5.4:28169666-28169685 None:intergenic 35.0%
!! GAAGTTTCTGTTTCTGATGA+GGG - chr5.4:28168906-28168925 None:intergenic 35.0%
!! TCATAATCAAGTACTGAGGT+GGG - chr5.4:28168807-28168826 None:intergenic 35.0%
!! TTCATAATCAAGTACTGAGG+TGG - chr5.4:28168808-28168827 None:intergenic 35.0%
AAATCTTGATGAGCAAGAGG+AGG - chr5.4:28168275-28168294 None:intergenic 40.0%
ACACAGGATAACACATTGCT+GGG - chr5.4:28169337-28169356 None:intergenic 40.0%
ACCTACAAGCATACTTCTAG+TGG - chr5.4:28169114-28169133 None:intergenic 40.0%
AGACCCTAATGAAGATGCAA+AGG + chr5.4:28169544-28169563 MS.gene032932:CDS 40.0%
ATAAGGCTTGAAGATGTGGT+AGG - chr5.4:28168867-28168886 None:intergenic 40.0%
ATGAGTAGTGTCTTGACCTT+TGG + chr5.4:28169194-28169213 MS.gene032932:CDS 40.0%
CCCAATATAGCATACTATGG+TGG + chr5.4:28169365-28169384 MS.gene032932:CDS 40.0%
CTCAATAAGGCTTGAAGATG+TGG - chr5.4:28168871-28168890 None:intergenic 40.0%
GACCCTAATGAAGATGCAAA+GGG + chr5.4:28169545-28169564 MS.gene032932:CDS 40.0%
GAGAAATCTTGATGAGCAAG+AGG - chr5.4:28168278-28168297 None:intergenic 40.0%
GGTTCTTCCGAAAAGCTTTA+TGG - chr5.4:28168207-28168226 None:intergenic 40.0%
TGAACAAAGTGATATGTCCC+AGG + chr5.4:28168950-28168969 MS.gene032932:CDS 40.0%
! AATGGATTTGCATCTATGGG+TGG + chr5.4:28169614-28169633 MS.gene032932:CDS 40.0%
! ACACAAGATTGAGTTGCGAT+AGG - chr5.4:28169419-28169438 None:intergenic 40.0%
! ACCACTAGAAGTATGCTTGT+AGG + chr5.4:28169110-28169129 MS.gene032932:CDS 40.0%
! GGTGCTGTGTGTTTGAAAAA+TGG - chr5.4:28168228-28168247 None:intergenic 40.0%
! TTTGTTAGGAGCTTTGTCCT+GGG - chr5.4:28168970-28168989 None:intergenic 40.0%
!! AGGAGTGTTTAAATCCTCTG+TGG - chr5.4:28168847-28168866 None:intergenic 40.0%
!! ATATTGGGTTGGAATGACAC+AGG - chr5.4:28169353-28169372 None:intergenic 40.0%
!! TTTTGTTAGGAGCTTTGTCC+TGG - chr5.4:28168971-28168990 None:intergenic 40.0%
!!! ATCCTCTGTGGTTTGTTTTG+AGG - chr5.4:28168835-28168854 None:intergenic 40.0%
!!! GTGTTTTCGGGTTCTCAATA+AGG - chr5.4:28168884-28168903 None:intergenic 40.0%
!!! GTTTCTGATGAGGGTGTTTT+CGG - chr5.4:28168897-28168916 None:intergenic 40.0%
!!! TTTCTGATGAGGGTGTTTTC+GGG - chr5.4:28168896-28168915 None:intergenic 40.0%
AAGAGGAGGAGAAAACTCTG+CGG - chr5.4:28168261-28168280 None:intergenic 45.0%
ACACTAGGAAACAAGAGTGG+TGG + chr5.4:28169581-28169600 MS.gene032932:CDS 45.0%
AGCCTCAAAACAAACCACAG+AGG + chr5.4:28168830-28168849 MS.gene032932:CDS 45.0%
ATAGGTTGTACGCTCCATGA+TGG - chr5.4:28169401-28169420 None:intergenic 45.0%
CATACTTCTAGTGGTTCCTC+CGG - chr5.4:28169105-28169124 None:intergenic 45.0%
CCTTGATAAAGCTGCAGGAT+TGG - chr5.4:28169684-28169703 None:intergenic 45.0%
GACACAGGATAACACATTGC+TGG - chr5.4:28169338-28169357 None:intergenic 45.0%
GCAAAAGCTGCCAGAGATAT+TGG + chr5.4:28169075-28169094 MS.gene032932:CDS 45.0%
GTGGTTGTTTAGTCCCATCA+TGG + chr5.4:28169384-28169403 MS.gene032932:CDS 45.0%
TAACACATTGCTGGGTTCCA+TGG - chr5.4:28169329-28169348 None:intergenic 45.0%
TAGGTTGTACGCTCCATGAT+GGG - chr5.4:28169400-28169419 None:intergenic 45.0%
TGAGACCTTGATAAAGCTGC+AGG - chr5.4:28169689-28169708 None:intergenic 45.0%
TGCCAGAGATATTGGACTTC+CGG + chr5.4:28169083-28169102 MS.gene032932:CDS 45.0%
TTGCATCTATGGGTGGTGAT+AGG + chr5.4:28169621-28169640 MS.gene032932:CDS 45.0%
! ACGCTTTTGCATCTTGGACA+TGG - chr5.4:28168999-28169018 None:intergenic 45.0%
! AGAAGTATGCTTGTAGGTGC+AGG + chr5.4:28169116-28169135 MS.gene032932:CDS 45.0%
! CCAATCCTGCAGCTTTATCA+AGG + chr5.4:28169681-28169700 MS.gene032932:CDS 45.0%
! GTGTCTACGCTTTTGCATCT+TGG - chr5.4:28169005-28169024 None:intergenic 45.0%
!! AGTGGTGGAGGACTTTTCAA+TGG + chr5.4:28169596-28169615 MS.gene032932:CDS 45.0%
CAGAGATATTGGACTTCCGG+AGG + chr5.4:28169086-28169105 MS.gene032932:CDS 50.0%
CTAGGAAACAAGAGTGGTGG+AGG + chr5.4:28169584-28169603 MS.gene032932:CDS 50.0%
CTCCGGAAGTCCAATATCTC+TGG - chr5.4:28169088-28169107 None:intergenic 50.0%
GGGTGGTGATAGGAATCATG+TGG + chr5.4:28169631-28169650 MS.gene032932:CDS 50.0%
GGGTTCCATGGAAACTGTTG+TGG - chr5.4:28169317-28169336 None:intergenic 50.0%
GGTCTCATGTGTTCCATGAG+AGG + chr5.4:28169702-28169721 MS.gene032932:CDS 50.0%
! CAGCTTTTGCAGAAGTGACG+GGG - chr5.4:28169065-28169084 None:intergenic 50.0%
! GCAGCTTTTGCAGAAGTGAC+GGG - chr5.4:28169066-28169085 None:intergenic 50.0%
! GGCAGCTTTTGCAGAAGTGA+CGG - chr5.4:28169067-28169086 None:intergenic 50.0%
!! TTTTGCAGAAGTGACGGGGC+TGG - chr5.4:28169061-28169080 None:intergenic 55.0%
! GCAGAAGTGACGGGGCTGGT+TGG - chr5.4:28169057-28169076 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 28168188 28169730 28168188 ID=MS.gene032932
chr5.4 mRNA 28168188 28169730 28168188 ID=MS.gene032932.t1;Parent=MS.gene032932
chr5.4 exon 28168188 28168382 28168188 ID=MS.gene032932.t1.exon1;Parent=MS.gene032932.t1
chr5.4 CDS 28168188 28168382 28168188 ID=cds.MS.gene032932.t1;Parent=MS.gene032932.t1
chr5.4 exon 28168786 28169730 28168786 ID=MS.gene032932.t1.exon2;Parent=MS.gene032932.t1
chr5.4 CDS 28168786 28169730 28168786 ID=cds.MS.gene032932.t1;Parent=MS.gene032932.t1
Gene Sequence

>MS.gene032932

ATGACTAATGATTCAACCATAAAGCTTTTCGGAAGAACCATTTTTCAAACACACAGCACCGATGATTATTCCGCAGAGTTTTCTCCTCCTCTTGCTCATCAAGATTTCTCTGATCATAGTCTTCATTCATCTTTATCTTCATCTTCTCCACTTGAAGTTAATTCTTCAAACGAACATGATTCAAAGAGATATAAGGTATGTTTCATGCTAATTTTTCATCTTTTTTTGTTTTAAAATTAATTACTTATAGTTAGGTGTATGCAAATGTGTATTTAAACTAATTTTCATTGCAATCTTGTAGCTATTTTGACTACTTTTTGCTTGAAAATATGCTTCATGAAATTGTGCACTTTTTTCATCTTTTTGGTGATAAAATTCTTATGGCTGTTTTGACAACTTTTTTTGCTTGAAAAATATACCAAGTTTTTGGATTTCATGATGACTGATGCTTGATGAAATTGTGCTTTTAAAGTTTTCAAGTTTTTCCAATTCATATTTTGAAGTCTATAATCCTAAACTGGTTTGATTGCAACTTCAACTTAACTTTGTGGTTCATATCTTGGTCTTACCTAATCTAATTATTGTTTATCAATTTCAGGAACAATCAAGGAAAGAACCCACCTCAGTACTTGATTATGAAGAAGCCTCAAAACAAACCACAGAGGATTTAAACACTCCTACCACATCTTCAAGCCTTATTGAGAACCCGAAAACACCCTCATCAGAAACAGAAACTTCTCAACAGAATTCCACTAAAATTGATGAACAAAGTGATATGTCCCAGGACAAAGCTCCTAACAAAATTGTTCCATGTCCAAGATGCAAAAGCGTAGACACAAAATTTTGTTACTACAATAATTACAACGCCAACCAGCCCCGTCACTTCTGCAAAAGCTGCCAGAGATATTGGACTTCCGGAGGAACCACTAGAAGTATGCTTGTAGGTGCAGGTCGCCGTAAGAATAAAATCTCTTCTTTTTCTTCCGATGTCTCACATAATCGTCAAATGAGTAGTGTCTTGACCTTTGGATCAGATTCTCCTATTATGTCTTCATCTTCACTTGTCAAGAAAATCAATATTGGATCAAATGATGAAACATCTGATAAGAGTAATCAGTGTTTTTTTCCACAACAGTTTCCATGGAACCCAGCAATGTGTTATCCTGTGTCATTCCAACCCAATATAGCATACTATGGTGGTTGTTTAGTCCCATCATGGAGCGTACAACCTATCGCAACTCAATCTTGTGTTCCAAGTAAACCTACTTTAGGGAAACATTCAAGAGATGGCTTAGAAAATAACAACAAAGAAAGTGATAATAATAGTGTGTTGATTCCTAAGAAATTGAGAATTGAAGACCCTAATGAAGATGCAAAGGGTTTTAATTGGTTAACACTAGGAAACAAGAGTGGTGGAGGACTTTTCAATGGATTTGCATCTATGGGTGGTGATAGGAATCATGTGGTTGAAGCAGCCTCATCAGTATTGAAAGCCAATCCTGCAGCTTTATCAAGGTCTCATGTGTTCCATGAGAGGATTTGA
Protein sequence

>MS.gene032932.t1

MTNDSTIKLFGRTIFQTHSTDDYSAEFSPPLAHQDFSDHSLHSSLSSSSPLEVNSSNEHDSKRYKEQSRKEPTSVLDYEEASKQTTEDLNTPTTSSSLIENPKTPSSETETSQQNSTKIDEQSDMSQDKAPNKIVPCPRCKSVDTKFCYYNNYNANQPRHFCKSCQRYWTSGGTTRSMLVGAGRRKNKISSFSSDVSHNRQMSSVLTFGSDSPIMSSSSLVKKINIGSNDETSDKSNQCFFPQQFPWNPAMCYPVSFQPNIAYYGGCLVPSWSVQPIATQSCVPSKPTLGKHSRDGLENNNKESDNNSVLIPKKLRIEDPNEDAKGFNWLTLGNKSGGGLFNGFASMGGDRNHVVEAASSVLKANPAALSRSHVFHERI